MD05G1203200.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003313
Physical Location & Seq
Reverse (-)
33343344 .. 33347931
4588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1203200.v1.1.491

Sequence Viewer

Length: 3006 bp
ATGGCAGCAAAGGGAAATCCATCTGTACATGTGCAGAAGAACTTAGAGGGGTTTGTAACCATTCTAACATCTGCAGCATGTGAATGGGTATTGATCTTTCTTCTGCTTGTTGACGGACTATTATCCTATCTGCTGACTAAATTTGCAGACTATTGCAATTTGCAAAAGCCTTGCATCTTTTGCTCCAGGTTTGAGCATGTTATAGGTAGTGAAAAACCTCAACTTTATCAGGATCTACTTTGCAGCAACCACGTATCTGAGTTCTCATCATGGTTCTCTTGTCAAATTTATGGTAAGCACGATCGCAGTGGAATGTGTGAAGATTGCCTCAAGTCATACACCAAAAAGGGCAAGTCCAACCCCGAAAAACACATGCTTTCGGGTGGTAAATCGGATTCTGATATTGGAAGTTCCAGTTCAAGGAGGTCCATGCAGAACAGAAAGTTTGTCCATAGTTCTGTGGGTGCAAGGACATGCTCTTGTTGCAGTAGGCCTTGTAGACCTAGAGAAAATGCTCGTGGACTACAACGAAAATCATCTGCTTCCAAACCTAATATCCCTTTGCCACGTTTTTCAAGCCGTAGTCGTATACCTCGTAGGGATGGTTCAAAGAAGACCAGGGATAAAACTTCTGGATCAGTAACTACTTGTCGTCCTGGAAAACCTGGCTGGGATCCCTTGCCACATAGAGGACACAGAGAGTTAAGGATTAGTTCTGATACTGACTCCACTCTTCGTCCTGGAAATTTTAGCTGGGATCCCTTACCACATGTAGGATACACTAAGTTGAGGATTAGTTCTGATACTGACTCCACTCGTCGTGCTGGAAAACTTGGCAGGGATCCCTTGTCACATGTAGGATACACTGAGTTGAAGATTAGTTCTGATACCGATTCTGAGATTCCATTTTCTGATGAAGACGGTGGTAGAAGCATAATTATTGGAAATGAGGAGCATCAGAAGGAAGCTTTAGTAGATCAATATGCTTCAGAAAAACCATTCAAAGCACAGCCAAATGGTTTGAATCCAGCAAAGCAGAGCAACATTACCACCAACCATAAGCCTTCACTTTCTGTTCCAAGTGTGCAGCCAGATGGTAGCAAGAGGTCTGATATAAAATTCTTAGGTTCGAATGTTGCTTCAGAGAATGGCTTGGATGAACTTAACTGGCAGCAAGCGAGTCAGAATTCCATCCCTTATGCATTCCCTGAGCTTATTTCACTGGATGATATCCCTCCATCATTTAATGTTGTTGAAAAGGGTTTTACTGGGACAAGTGACTTTGGAGATGCATCCATCAGTAAAAACGATGAGCTTTTGAGGTCTATAAGTGCAACAGATGGAGCACCTATTAAAACTGATCTTATCATCAACGATCCAGATCTTACTAAGCCAAATCATACTAATGGAAATGCTGTGTCTGAGTCAGCAGGCATTGGTAAGGAAGGAGATTCTATTAAAGAACAACCTCCAGTGAAGGTACTTGATAGAGTTGATGGGGAAGTGAAACCAGCGCACTCACAAAGTTCTTCTGCTCAGGAGACAAATTTATCATCAAATAATACAATTACAGGGGTACAAGGTGTTGGTGATGAAAAACCGGTAGCTGATGCTTCCAATTCTTCTGCTCAGGAGAGTAATTTATCATCACATAAAACAAGTTCAGCGGTACTTGGTGTTGGTGATAAAACACTGGTAACTGATGCTTCCAAATCTTCTGCTCAGGAGAGTAATTTATCATCAAATAATACAATTTCAGCGGTATGTGGTGCTGGTGATGAAAAGCCGGTACCAGATGCTTCCAACTCTTCTGCTCACAACTGTAGTTTATCATCAAATAATACAATTTCAGGGGTACGTGGTGGTGGTGATGAAAAGCAGGTAACTGATGCTTCCAATTCTAATCCAAATCTGGGGCTTAAAACATCATGGTCAGTAGAATCTCTGGATGGAGGATATGTCAGTGAAATTGAAGGTGAAAGTATTGTTGATCGGTTAAAACGACAGGTTGATTATTATAGAAAAAGCTTAAAGGAGTTGTTTAAGGAACTAGACGAAGAAAGGAATGCCGCTGAAATTGCTGCAAATCAGGCAATGGCCATGATCACAAAGCTACAAGAAGAGAAAGCTGCAATCCATATGGAGGCCCTTCAGTACTTAAGAATGATGGAAGAGCAGGCTGAGTTTGATGTAGATGCACTTGAAAAGGCCAATGATCTCCTAGCTGAAAAGGAGAAACATATACAGGACTTGGAAGCAGAGCTAGAATTTTCTAACTTCGATATCCCAGATGAATCAACAATGGAGAGTATGCCAATGCCGGAATTGCCAGCATTCCCAGTATTGCAGGGAAAAAGTTGTAATTTGAAAGGTGAGATTGACACAGTGGAGAGTACTGTTGTTCCCGGTGTAAAAAGTGATGTCAAAGTTACTGGTAACTCAACAGTTACTGAGGTACCTAAAGCTAGCAATGAACGCCTTTTTTCTGAGACAACACTTTTAGAATTTGAAGATGAAAAGTTGTACATTTCAAATTGTTTGAAGAGCTTGGAGAGGAAGCTTTCCGAGATTTCCTGTAATGGGGCTTCTACCAGCATGCCTAACGGTGGGCATTCTAGAAAGCATACATATGATGGACCAGATCAAGTAGAGACGCCTAGAAAATTTAGGCTTCTATTAAATGGTCAGATTGAAGAGGAAGTTGTGCTGACACCGAATGATTTACACATATCTAACGGAAGTGCTGCTCCTCAAGAGGGACCAATTCATTCCGATAGCTCAGACTGTGAAGAGGAAAAAGATTCAGATTTGGTTGTTCTGGAAAACGAAATCTCAGACCTTAATGACAGGTTGGAGGCACTCGAGACTGATCATGATTTTCTTGAGCACATGCTTTACTCTCTTCAAAATGGACAGGAAGGGCTGCAGTTTATTCAAGAGATAGCTCAGCAGCTGCGAGAACTGCGAAAAATTGGTGCAGCTTTCAGAAATCAGTCTGTTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

1002

Amino Acids

109.4

Weight (kDa)

5.16

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 665 - 755 2.1e-28 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1870
Acc65I GGTACC 2 cut(s) 1789, 2455
AccB1I GGYRCC 2 cut(s) 1789, 2455
AccI GTMKAC 2 cut(s) 499, 589
AciI CCGC 3 cut(s) 1667, 1760, 2070
AclWI GGATC 9 cut(s) 240, 643, 668, 681, 752, 765, 836, 849, 1370
AcoI YGGCCR 1 cut(s) 2097
AcsI RAATTY 9 cut(s) 140, 285, 745, 1118, 1186, 1546, 2267, 2504, 2663
AcuI CTGAAG 3 cut(s) 972, 1125, 2135
AcyI GRCGYC 1 cut(s) 2654
AfiI CCNNNNNNNGG 8 cut(s) 420, 689, 773, 1913, 2143, 2580, 2606, 2756
AflII CTTAAG 1 cut(s) 2158
AflIII ACRYGT 3 cut(s) 28, 769, 853
AgeI ACCGGT 1 cut(s) 1600
AjnI CCWGG 5 cut(s) 185, 617, 655, 664, 739
Alw21I GWGCWC 2 cut(s) 1348, 2889
Alw26I GTCTC 4 cut(s) 1535, 2483, 2645, 2858
AlwI GGATC 9 cut(s) 240, 643, 668, 681, 752, 765, 836, 849, 1370
AlwNI CAGNNNCTG 2 cut(s) 2450, 2953
Ama87I CYCGRG 1 cut(s) 2861
AoxI GGCC 4 cut(s) 491, 2097, 2145, 2208
ApoI RAATTY 9 cut(s) 140, 285, 745, 1118, 1186, 1546, 2267, 2504, 2663
AsiGI ACCGGT 1 cut(s) 1600
Asp718I GGTACC 2 cut(s) 1789, 2455
AspLEI GCGC 1 cut(s) 1516
AspS9I GGNCC 4 cut(s) 426, 2146, 2636, 2759
AsuC2I CCSGG 1 cut(s) 2406
AsuHPI GGTGA 6 cut(s) 1601, 1694, 1787, 1880, 1988, 2384
AsuII TTCGAA 1 cut(s) 1130
AsuNHI GCTAGC 1 cut(s) 2465
AvaI CYCGRG 1 cut(s) 2861
AvaII GGWCC 3 cut(s) 426, 2636, 2759
BaeI ACNNNNGTAYC 2 cut(s) 2439, 2472
BalI TGGCCA 1 cut(s) 2099
BamHI GGATCC 3 cut(s) 673, 757, 841
BanI GGYRCC 2 cut(s) 1789, 2455
BarI GAAGNNNNNNTAC 2 cut(s) 2569, 2601
BauI CACGAG 1 cut(s) 516
BbsI GAAGAC 2 cut(s) 620, 924
Bbv12I GWGCWC 2 cut(s) 1348, 2889
BceAI ACGGC 1 cut(s) 564
BciT130I CCWGG 5 cut(s) 187, 619, 657, 666, 741
BciVI GTATCC 2 cut(s) 770, 854
BclI TGATCA 2 cut(s) 2103, 2869
BcnI CCSGG 1 cut(s) 2406
BcoDI GTCTC 4 cut(s) 1535, 2483, 2645, 2858
BfaI CTAG 7 cut(s) 504, 2051, 2222, 2264, 2466, 2616, 2658
BfmI CTRYAG 3 cut(s) 72, 1822, 2924
BfrI CTTAAG 1 cut(s) 2158
BfuAI ACCTGC 1 cut(s) 1870
BfuI GTATCC 2 cut(s) 770, 854
BglII AGATCT 1 cut(s) 1381
BlpI GCTNAGC 1 cut(s) 2946
BmcAI AGTACT 2 cut(s) 2156, 2395
Bme1390I CCNGG 6 cut(s) 187, 619, 657, 666, 741, 2406
Bme18I GGWCC 3 cut(s) 426, 2636, 2759
BmeT110I CYCGRG 1 cut(s) 2861
BmgT120I GGNCC 4 cut(s) 426, 2146, 2636, 2759
BmiI GGNNCC 6 cut(s) 675, 759, 843, 1791, 2457, 2760
BmrFI CCNGG 6 cut(s) 187, 619, 657, 666, 741, 2406
BmrI ACTGGG 2 cut(s) 1278, 2333
BmsI GCATC 9 cut(s) 183, 964, 1279, 1301, 1600, 1693, 1786, 1879, 2185
BmtI GCTAGC 1 cut(s) 2469
BmuI ACTGGG 2 cut(s) 1278, 2333
BpiI GAAGAC 2 cut(s) 620, 924
BpmI CTGGAG 2 cut(s) 169, 1455
Bpu10I CCTNAGC 4 cut(s) 1209, 1536, 1629, 1722
Bpu1102I GCTNAGC 1 cut(s) 2946
Bpu14I TTCGAA 1 cut(s) 1130
BpuEI CTTGAG 3 cut(s) 314, 2736, 2903
BpuMI CCSGG 1 cut(s) 2406
BsaAI YACGTR 2 cut(s) 253, 1859
BsaBI GATNNNNATC 1 cut(s) 1380
BsaHI GRCGYC 1 cut(s) 2654
BsaJI CCNNGG 1 cut(s) 618
BsaWI WCCGGW 1 cut(s) 1600
BsaXI ACNNNNNCTCC 2 cut(s) 1944, 1974
Bsc4I CCNNNNNNNGG 8 cut(s) 420, 689, 773, 1913, 2143, 2580, 2606, 2756
Bse118I RCCGGY 2 cut(s) 1600, 1786
Bse1I ACTGG 8 cut(s) 414, 1172, 1227, 1273, 1472, 1698, 2339, 2437
Bse3DI GCAATG 2 cut(s) 2100, 2476
Bse8I GATNNNNATC 1 cut(s) 1380
BseBI CCWGG 5 cut(s) 187, 619, 657, 666, 741
BseDI CCNNGG 1 cut(s) 618
BseGI GGATG 6 cut(s) 607, 1162, 1191, 1231, 1292, 1954
BseJI GATNNNNATC 1 cut(s) 1380
BseLI CCNNNNNNNGG 8 cut(s) 420, 689, 773, 1913, 2143, 2580, 2606, 2756
BseMI GCAATG 2 cut(s) 2100, 2476
BseNI ACTGG 8 cut(s) 414, 1172, 1227, 1273, 1472, 1698, 2339, 2437
BseRI GAGGAG 2 cut(s) 965, 2739
BseYI CCCAGC 2 cut(s) 669, 753
BsgI GTGCAG 3 cut(s) 53, 1106, 2997
Bsh1285I CGRYCG 1 cut(s) 304
BshFI GGCC 4 cut(s) 493, 2099, 2147, 2210
BshNI GGYRCC 2 cut(s) 1789, 2455
BshTI ACCGGT 1 cut(s) 1600
BsiEI CGRYCG 1 cut(s) 304
BsiHKAI GWGCWC 2 cut(s) 1348, 2889
BsiHKCI CYCGRG 1 cut(s) 2861
BsiSI CCGG 4 cut(s) 1601, 1787, 2321, 2406
BslFI GGGAC 2 cut(s) 1285, 2772
BslI CCNNNNNNNGG 8 cut(s) 420, 689, 773, 1913, 2143, 2580, 2606, 2756
BsmAI GTCTC 4 cut(s) 1535, 2483, 2645, 2858
BsmBI CGTCTC 1 cut(s) 2645
BsmFI GGGAC 2 cut(s) 1285, 2772
BsmI GAATGC 4 cut(s) 1202, 2071, 2333, 2611
BsnI GGCC 4 cut(s) 493, 2099, 2147, 2210
BsoBI CYCGRG 1 cut(s) 2861
Bsp119I TTCGAA 1 cut(s) 1130
Bsp1286I GDGCHC 2 cut(s) 1348, 2889
Bsp1407I TGTACA 2 cut(s) 25, 2523
Bsp1720I GCTNAGC 1 cut(s) 2946
BspACI CCGC 3 cut(s) 1667, 1760, 2070
BspANI GGCC 4 cut(s) 493, 2099, 2147, 2210
BspHI TCATGA 1 cut(s) 2872
BspLI GGNNCC 6 cut(s) 675, 759, 843, 1791, 2457, 2760
BspMAI CTGCAG 2 cut(s) 76, 2928
BspMI ACCTGC 1 cut(s) 1870
BspOI GCTAGC 1 cut(s) 2469
BspPI GGATC 9 cut(s) 240, 643, 668, 681, 752, 765, 836, 849, 1370
BspQI GCTCTTC 2 cut(s) 2166, 2537
BspT104I TTCGAA 1 cut(s) 1130
BspT107I GGYRCC 2 cut(s) 1789, 2455
BspTI CTTAAG 1 cut(s) 2158
BsrDI GCAATG 2 cut(s) 2100, 2476
BsrFI RCCGGY 2 cut(s) 1600, 1786
BsrGI TGTACA 2 cut(s) 25, 2523
BsrI ACTGG 8 cut(s) 414, 1172, 1227, 1273, 1472, 1698, 2339, 2437
BssAI RCCGGY 2 cut(s) 1600, 1786
BssECI CCNNGG 1 cut(s) 618
BssNAI GTATAC 1 cut(s) 590
BssNI GRCGYC 1 cut(s) 2654
BssSI CACGAG 1 cut(s) 516
Bst1107I GTATAC 1 cut(s) 590
Bst2BI CACGAG 1 cut(s) 516
Bst2UI CCWGG 5 cut(s) 187, 619, 657, 666, 741
Bst4CI ACNGT 7 cut(s) 923, 1823, 2386, 2398, 2446, 2606, 2786
Bst6I CTCTTC 8 cut(s) 738, 1813, 2115, 2166, 2537, 2688, 2784, 2907
BstACI GRCGYC 1 cut(s) 2654
BstAFI CTTAAG 1 cut(s) 2158
BstAPI GCANNNNNTGC 1 cut(s) 180
BstAUI TGTACA 2 cut(s) 25, 2523
BstBAI YACGTR 2 cut(s) 253, 1859
BstBI TTCGAA 1 cut(s) 1130
BstC8I GCNNGC 6 cut(s) 1176, 1432, 2178, 2331, 2467, 2597
BstF5I GGATG 6 cut(s) 607, 1162, 1191, 1231, 1292, 1954
BstHHI GCGC 1 cut(s) 1516
BstMAI GTCTC 4 cut(s) 1535, 2483, 2645, 2858
BstMCI CGRYCG 1 cut(s) 304
BstMWI GCNNNNNNNGC 7 cut(s) 180, 483, 2078, 2090, 2326, 2475, 2962
BstNI CCWGG 5 cut(s) 187, 619, 657, 666, 741
BstNSI RCATGY 9 cut(s) 32, 81, 200, 376, 477, 773, 857, 2599, 2893
BstSCI CCNGG 6 cut(s) 185, 617, 655, 664, 739, 2404
BstSFI CTRYAG 3 cut(s) 72, 1822, 2924
BstV2I GAAGAC 2 cut(s) 620, 924
BstX2I RGATCY 5 cut(s) 232, 673, 757, 841, 1381
BstYI RGATCY 5 cut(s) 232, 673, 757, 841, 1381
BstZ17I GTATAC 1 cut(s) 590
BsuI GTATCC 2 cut(s) 770, 854
BsuRI GGCC 4 cut(s) 493, 2099, 2147, 2210
BtsCI GGATG 6 cut(s) 607, 1162, 1191, 1231, 1292, 1954
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 7 cut(s) 313, 864, 1220, 1479, 1691, 1969, 2391
BveI ACCTGC 1 cut(s) 1870
Cac8I GCNNGC 6 cut(s) 1176, 1432, 2178, 2331, 2467, 2597
CaiI CAGNNNCTG 2 cut(s) 2450, 2953
CciI TCATGA 1 cut(s) 2872
CfoI GCGC 1 cut(s) 1516
Cfr10I RCCGGY 2 cut(s) 1600, 1786
Cfr13I GGNCC 4 cut(s) 426, 2146, 2636, 2759
CseI GACGC 1 cut(s) 2662
CspAI ACCGGT 1 cut(s) 1600
EaeI YGGCCR 1 cut(s) 2097
Eam1104I CTCTTC 8 cut(s) 738, 1813, 2115, 2166, 2537, 2688, 2784, 2907
EarI CTCTTC 8 cut(s) 738, 1813, 2115, 2166, 2537, 2688, 2784, 2907
Eco147I AGGCCT 1 cut(s) 493
Eco32I GATATC 2 cut(s) 1231, 2284
Eco47I GGWCC 3 cut(s) 426, 2636, 2759
Eco57I CTGAAG 3 cut(s) 972, 1125, 2135
Eco88I CYCGRG 1 cut(s) 2861
EcoO109I RGGNCCY 1 cut(s) 2146
EcoRI GAATTC 1 cut(s) 1186
EcoRII CCWGG 5 cut(s) 185, 617, 655, 664, 739
EcoRV GATATC 2 cut(s) 1231, 2284
EcoT22I ATGCAT 2 cut(s) 1204, 1294
Esp3I CGTCTC 1 cut(s) 2645
FaqI GGGAC 2 cut(s) 1285, 2772
FauNDI CATATG 2 cut(s) 2139, 2629
FbaI TGATCA 2 cut(s) 2103, 2869
FblI GTMKAC 2 cut(s) 499, 589
FokI GGATG 6 cut(s) 614, 1169, 1178, 1238, 1279, 1961
FspBI CTAG 7 cut(s) 504, 2051, 2222, 2264, 2466, 2616, 2658
GlaI GCGC 1 cut(s) 1515
GsaI CCCAGC 2 cut(s) 673, 757
GsuI CTGGAG 2 cut(s) 169, 1455
HaeIII GGCC 4 cut(s) 493, 2099, 2147, 2210
HapII CCGG 4 cut(s) 1601, 1787, 2321, 2406
HgaI GACGC 1 cut(s) 2662
HhaI GCGC 1 cut(s) 1516
Hin1I GRCGYC 1 cut(s) 2654
Hin6I GCGC 1 cut(s) 1514
HinP1I GCGC 1 cut(s) 1514
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 3 cut(s) 966, 2026, 2558
HpaII CCGG 4 cut(s) 1601, 1787, 2321, 2406
HphI GGTGA 6 cut(s) 1601, 1694, 1787, 1880, 1988, 2384
Hpy166II GTNNAC 4 cut(s) 112, 500, 521, 590
Hpy8I GTNNAC 4 cut(s) 112, 500, 521, 590
Hpy99I CGWCG 1 cut(s) 822
HpyAV CCTTC 7 cut(s) 955, 1074, 1439, 1471, 1967, 2159, 2912
HpyCH4III ACNGT 7 cut(s) 923, 1823, 2386, 2398, 2446, 2606, 2786
HpyCH4IV ACGT 3 cut(s) 252, 568, 1858
HpyF10VI GCNNNNNNNGC 7 cut(s) 180, 483, 2078, 2090, 2326, 2475, 2962
HpySE526I ACGT 3 cut(s) 252, 568, 1858
Hsp92I GRCGYC 1 cut(s) 2654
HspAI GCGC 1 cut(s) 1514
KpnI GGTACC 2 cut(s) 1793, 2459
Ksp22I TGATCA 2 cut(s) 2103, 2869
LguI GCTCTTC 2 cut(s) 2166, 2537
LmnI GCTCC 4 cut(s) 188, 952, 1343, 2752
LweI GCATC 9 cut(s) 183, 964, 1279, 1301, 1600, 1693, 1786, 1879, 2185
MaeI CTAG 7 cut(s) 504, 2051, 2222, 2264, 2466, 2616, 2658
MaeII ACGT 3 cut(s) 252, 568, 1858
MaeIII GTNAC 9 cut(s) 55, 640, 849, 1277, 1696, 1882, 2428, 2435, 2446
MflI RGATCY 5 cut(s) 232, 673, 757, 841, 1381
MhlI GDGCHC 2 cut(s) 1348, 2889
MlsI TGGCCA 1 cut(s) 2099
MluNI TGGCCA 1 cut(s) 2099
MlyI GAGTC 4 cut(s) 719, 803, 1189, 1433
MmeI TCCRAC 3 cut(s) 381, 1827, 2832
Mox20I TGGCCA 1 cut(s) 2099
Mph1103I ATGCAT 2 cut(s) 1204, 1294
MscI TGGCCA 1 cut(s) 2099
MslI CAYNNNNRTG 3 cut(s) 82, 1404, 2628
Msp20I TGGCCA 1 cut(s) 2099
MspA1I CMGCKG 4 cut(s) 1667, 1760, 2072, 2953
MspCI CTTAAG 1 cut(s) 2158
MspI CCGG 4 cut(s) 1601, 1787, 2321, 2406
MspR9I CCNGG 6 cut(s) 187, 619, 657, 666, 741, 2406
Mva1269I GAATGC 4 cut(s) 1202, 2071, 2333, 2611
MvaI CCWGG 5 cut(s) 187, 619, 657, 666, 741
MwoI GCNNNNNNNGC 7 cut(s) 180, 483, 2078, 2090, 2326, 2475, 2962
NciI CCSGG 1 cut(s) 2406
NdeI CATATG 2 cut(s) 2139, 2629
NheI GCTAGC 1 cut(s) 2465
NlaIV GGNNCC 6 cut(s) 675, 759, 843, 1791, 2457, 2760
NmuCI GTSAC 2 cut(s) 849, 1277
NsiI ATGCAT 2 cut(s) 1204, 1294
NspI RCATGY 9 cut(s) 32, 81, 200, 376, 477, 773, 857, 2599, 2893
NspV TTCGAA 1 cut(s) 1130
PaeI GCATGC 1 cut(s) 2599
PaeR7I CTCGAG 1 cut(s) 2861
PagI TCATGA 1 cut(s) 2872
PceI AGGCCT 1 cut(s) 493
PciI ACATGT 3 cut(s) 28, 769, 853
PciSI GCTCTTC 2 cut(s) 2166, 2537
PcsI WCGNNNNNNNCGW 2 cut(s) 592, 1999
PctI GAATGC 4 cut(s) 1202, 2071, 2333, 2611
PfeI GAWTC 8 cut(s) 395, 893, 901, 1024, 1451, 1940, 2294, 2801
PfoI TCCNGGA 2 cut(s) 655, 739
PinAI ACCGGT 1 cut(s) 1600
Ple19I CGATCG 1 cut(s) 304
PleI GAGTC 4 cut(s) 719, 803, 1188, 1432
PpsI GAGTC 4 cut(s) 719, 803, 1188, 1432
Ppu21I YACGTR 2 cut(s) 253, 1859
PscI ACATGT 3 cut(s) 28, 769, 853
Psp6I CCWGG 5 cut(s) 185, 617, 655, 664, 739
PspFI CCCAGC 2 cut(s) 669, 753
PspGI CCWGG 5 cut(s) 185, 617, 655, 664, 739
PspN4I GGNNCC 6 cut(s) 675, 759, 843, 1791, 2457, 2760
PspPI GGNCC 4 cut(s) 426, 2146, 2636, 2759
PsrI GAACNNNNNNTAC 2 cut(s) 589, 621
PstI CTGCAG 2 cut(s) 76, 2928
PstNI CAGNNNCTG 2 cut(s) 2450, 2953
PsuI RGATCY 5 cut(s) 232, 673, 757, 841, 1381
PvuI CGATCG 1 cut(s) 304
PvuII CAGCTG 1 cut(s) 2953
RseI CAYNNNNRTG 3 cut(s) 82, 1404, 2628
SapI GCTCTTC 2 cut(s) 2166, 2537
Sau96I GGNCC 4 cut(s) 426, 2146, 2636, 2759
ScaI AGTACT 2 cut(s) 2156, 2395
SchI GAGTC 4 cut(s) 719, 803, 1189, 1433
ScrFI CCNGG 6 cut(s) 187, 619, 657, 666, 741, 2406
SduI GDGCHC 2 cut(s) 1348, 2889
SfaNI GCATC 9 cut(s) 183, 964, 1279, 1301, 1600, 1693, 1786, 1879, 2185
SfcI CTRYAG 3 cut(s) 72, 1822, 2924
Sfr274I CTCGAG 1 cut(s) 2861
SfuI TTCGAA 1 cut(s) 1130
SinI GGWCC 3 cut(s) 426, 2636, 2759
SlaI CTCGAG 1 cut(s) 2861
SmiMI CAYNNNNRTG 3 cut(s) 82, 1404, 2628
SmlI CTYRAG 5 cut(s) 329, 2158, 2751, 2861, 2882
SmoI CTYRAG 5 cut(s) 329, 2158, 2751, 2861, 2882
SphI GCATGC 1 cut(s) 2599
SseBI AGGCCT 1 cut(s) 493
SsiI CCGC 3 cut(s) 1667, 1760, 2070
SspMI CTAG 7 cut(s) 504, 2051, 2222, 2264, 2466, 2616, 2658
StuI AGGCCT 1 cut(s) 493
StyD4I CCNGG 6 cut(s) 185, 617, 655, 664, 739, 2404
TaaI ACNGT 7 cut(s) 923, 1823, 2386, 2398, 2446, 2606, 2786
TaiI ACGT 3 cut(s) 255, 571, 1861
TaqI TCGA 3 cut(s) 1130, 2280, 2862
TatI WGTACW 4 cut(s) 25, 2154, 2393, 2523
TauI GCSGC 1 cut(s) 2072
TfiI GAWTC 8 cut(s) 395, 893, 901, 1024, 1451, 1940, 2294, 2801
TscAI CASTG 7 cut(s) 313, 871, 1227, 1479, 1698, 1969, 2391
TseFI GTSAC 2 cut(s) 849, 1277
Tsp45I GTSAC 2 cut(s) 849, 1277
TspDTI ATGAA 9 cut(s) 930, 1173, 1608, 1794, 1887, 2307, 2487, 2529, 2756
TspGWI ACGGA 2 cut(s) 129, 2751
TspRI CASTG 7 cut(s) 313, 871, 1227, 1479, 1698, 1969, 2391
Vha464I CTTAAG 1 cut(s) 2158
VpaK11BI GGWCC 3 cut(s) 426, 2636, 2759
XapI RAATTY 9 cut(s) 140, 285, 745, 1118, 1186, 1546, 2267, 2504, 2663
XbaI TCTAGA 1 cut(s) 2615
XceI RCATGY 9 cut(s) 32, 81, 200, 376, 477, 773, 857, 2599, 2893
XhoI CTCGAG 1 cut(s) 2861
XmiI GTMKAC 2 cut(s) 499, 589
XspI CTAG 7 cut(s) 504, 2051, 2222, 2264, 2466, 2616, 2658
ZrmI AGTACT 2 cut(s) 2156, 2395
Zsp2I ATGCAT 2 cut(s) 1204, 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.