Rw6G026970

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003313
Physical Location & Seq
Reverse (-)
50685269 .. 50689657
4389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G026970.1

Sequence Viewer

Length: 2913 bp
ATGGCAGCAAAGGGGAAGTCATCAGTACAAGTGCAGAGGAACTTTGAGAGGTTTATAGCCATTCTAACATCTGCAGCATGTGAATGGTTTTTGATATTTCTTCTGTTTGTTGATGCACTGTTATCCTATCTGCTGACTAAGTTTGCGGTCTATTGCGACTTGCAAAAGCCCTGCATCTTGTGCTCAAGGTTTGAACATATTACTGGTAATGAAAGTTCTCAACTTTATGAGGATCTACTTTGCAGCAACCACATATCGAAGCTCACATCTTGGATAGCTAGTCAAATTTATGGCAAGGATGATGGCAATGGAATGCATGAAGATTATCTCTTTCCTTTCACCACAAGGGACAACTCCAACCGAGAAATGCATATGTTTTCAGTTGGTAATTCGGTATCAGATGTTGAAAGTTATGGTTCTCGGAGGCGTGTGCCAAGCAGAAGTTGTGTTCACAGTCCTGTTCACAGTTCTGTGGGTGTAGGGCAATGGTCTTGTTGCTGTAGTCTTTGGGGACATAGAGGAAAGTTTAAAGGACTAAAACTCAAATCATCTGGGACAAAACATGCCAAGCCTAATATTCCTTTGCCACGTTTGCCAAACAGTAGCCGTTCAAATCGAAGCCGGGATAGTTCAAAGAACATCAGGGATAAGTCTCCGGCGTCAGTCACTAGTCGTCGTTCTGCAAAAGTGGGAGTTGATCCCTTGTTTCATGTAGGATACAGTGAATTGAAGTTCTTTTCTGATACTGACTCAGAGTTTCTGTTTTCTGATGGGGATGATGGTAGCGGTTTAACCAGTGTAAATCATGAGCCTAAGATGGAAGGTTTAGTACGTGCTTCACGATATCGGCCTAAAGCACCACCAAATGGTTTGAGGTCAGGAAGGCCTAGCAAGCGTCCCCCCAAACCCAAGCGGTCACTTTCAGAACCATGGCCTGTTTTTAGGAAAGCCTATGATGTAAAATCCTTGGGTTCTTATAACGAATTTGAGACTGGCCTCAGTGAGCTTAACTGTCAACAATCCAATAGGAAGTCCAACCCTTCTGCATTACCTGAGCTCATTTCACTAGATGACATCCTTTTGTCACCCAATGTTCTAGAAAAGGCAGATATTAATGGGACAGGGGACTTTGGATATGCATCTATCAATAAGAATGTTGAACTTCTGAAGTCTGTATGTGCAACAGACGGAGCACCTGTTAAAACCGATCCAGCTCTTATAAAACCAAACCATAGTAAGGTAGATGCTGTGTCTGAGTCAGAGGCAAGTGTTAGGGAAAGAGATTCATCTGGTACTACTACAGAAGCTCCTCTTATGAAACCAAACCCTAGTAATGTAAATGCTGTGTCCAAGTCACAGGGAAGTGTTCGAGAAAGAGATTCATCTGGTATTACTACTGAAGCTTTTATAAAACCAGACCATAATAAGGTAAATGCTGTGTCACAGTCAGAGGGAAATGTTAAGGAAAGAACTTCATCCGGTATTACTACAGAAGCTCTTATACAACCAGAACATGGTAAGGTAAATCCTCTGTCTCAGTCAGACAGAAATGTTAAGGAAAGAGATTCTTCTGGTGTTTCTAAAGAACAGCCAGCAGTGAAGGAACCCGATAGAGTTGATGAGGAAATGGAAGTGCTAGCTTCACAGGACTCTTCTGCTCGGGAGACTAACTCATCAGCAAAGAATACAAGTTCAACAGTGGATGGTTGTGTGGGTGAAAAACAGACAACTGATGCCTCCAGCTCTAATGCAAAGCCGATGCTTCACACGTCAGCATCAGTAGAATCTGGTCTTGAATCACTGGATGGAGGCTATGTCAGTGAAATTGAAGGTGAAAGTATAGTTGATCAGTTAAAACGACAGATTGATTATGACAGGAAATGCATAAAGGAGTTGTATAAGGAATTGGAAGAAGAAAGGAGTGCCGCTGCCATTGCTGCAAATCAGGCAATGGCCATGATCACAAAGCTCCAAGAAGAGAAAGCTGCATTCCATATGGAGGCCCTTCACTGCTTAAGAATGATGGAAGAGCAGGCTGAGTTTGACGTAGAGGCACTGGAGAAAGCCAATGATCTCCTGGCTGAGAAGGAGAAAGAGATACAGGACTTGGAGGCAGAGCTAGAATTTTCTAAGTTAGACTTCCAAGATGAATCGATAATGGAGAATCCACTTGACAAAAGTTGTGATTTGATGGGGGGGATTGACCCGGTGGAGAATACTGTTATGCCCTGTGTAAAAAACGATTTCAATGTTACTTGCAACTCAATGGCTACTGAGGTATCCATAGGCAGTAACCAACCTCCTTTTTCTGAAACTACTCTTTTAGAGTTTGAAGATGAAAAATTGTACATTACAAATTGTTTGAAGAGCTTGGAGAGGAAGCTTTCTGAACTTTCCAGTAGAGGGGCTTCCTCCAAAATGCTTAATGGTGGGCATTCCCAAAAGCATATGGATGATGGACAAAATCAAGCAGAGACTCCTAGAAAAGAGAGGAGTCTACTAAATGATGGACAAAATCAAGAAGAGACTCCTAGGAAAGGGAGGAGTCTATTAAATGGTCAGCTTGAAGAGAATGTTCTGCATGGGCAAAAAGATTTACATATGTCTAATGGTAGCTCTGCTGCTCAAGATGGGCCAACTGGTTGTGGTGGTGGTGATGAATTAGAATGTGAAGACAATAATGCTGATTCCCGTGGGGAAAAAGATATCATGGATCCTGGAGAAATGGATTTGGTTATTCTGGAAAATGAAATCTCAGACCTTAATGATAGGTTGGAGGCACTTGAGACTGATCATGATTTTCTTGAGCACATGCTCTACTCTCTTCAAAATGGAGCTGAAGGACTACAATTTGTTCGAGAGATAGCTCATCAGCTGCAAGAGTTGCGGAAGATAGGGACGGGTTTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

970

Amino Acids

107.02

Weight (kDa)

5.16

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 617 - 707 2.8e-28 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 978, 1220, 1409
AccB7I CCANNNNNTGG 2 cut(s) 866, 1514
AccI GTMKAC 1 cut(s) 2497
AciI CCGC 5 cut(s) 146, 786, 913, 1926, 2887
AclWI GGATC 5 cut(s) 240, 692, 1202, 2708, 2721
AcoI YGGCCR 1 cut(s) 1953
AcsI RAATTY 3 cut(s) 285, 983, 2123
AcuI CTGAAG 3 cut(s) 1187, 1419, 2859
AcyI GRCGYC 1 cut(s) 659
AfaI GTAC 4 cut(s) 27, 831, 1294, 2348
AfiI CCNNNNNNNGG 7 cut(s) 866, 1237, 1426, 1514, 1999, 2403, 2537
AflII CTTAAG 1 cut(s) 2014
AflIII ACRYGT 1 cut(s) 1767
AhlI ACTAGT 1 cut(s) 668
AjiI CACGTC 1 cut(s) 1770
AjnI CCWGG 2 cut(s) 2076, 2716
Alw21I GWGCWC 4 cut(s) 185, 1059, 1195, 2811
Alw26I GTCTC 7 cut(s) 657, 983, 1539, 1658, 2468, 2519, 2780
AlwI GGATC 5 cut(s) 240, 692, 1202, 2708, 2721
Ama87I CYCGRG 1 cut(s) 1659
AoxI GGCC 7 cut(s) 848, 884, 932, 994, 1953, 2001, 2633
ApeKI GCWGC 8 cut(s) 5, 74, 243, 1928, 1937, 1985, 2621, 2875
ApoI RAATTY 3 cut(s) 285, 983, 2123
ArsI GACNNNNNNTTYG 1 cut(s) 34
AseI ATTAAT 1 cut(s) 1113
AspA2I CCTAGG 1 cut(s) 2531
AspS9I GGNCC 2 cut(s) 2002, 2633
AsuC2I CCSGG 2 cut(s) 623, 2207
AsuHPI GGTGA 5 cut(s) 331, 1077, 1727, 1844, 2666
AsuNHI GCTAGC 1 cut(s) 1636
AvaI CYCGRG 1 cut(s) 1659
AvrII CCTAGG 1 cut(s) 2531
BaeI ACNNNNGTAYC 2 cut(s) 2262, 2295
BalI TGGCCA 1 cut(s) 1955
BamHI GGATCC 1 cut(s) 2713
BanII GRGCYC 1 cut(s) 1059
BarI GAAGNNNNNNTAC 6 cut(s) 813, 845, 1485, 1517, 2392, 2424
BbsI GAAGAC 1 cut(s) 2679
Bbv12I GWGCWC 4 cut(s) 185, 1059, 1195, 2811
BbvI GCAGC 8 cut(s) 17, 86, 255, 1915, 1924, 1972, 2608, 2862
BceAI ACGGC 1 cut(s) 591
BciT130I CCWGG 2 cut(s) 2078, 2718
BciVI GTATCC 2 cut(s) 710, 2290
BclI TGATCA 3 cut(s) 1846, 1959, 2791
BcnI CCSGG 2 cut(s) 623, 2207
BcoDI GTCTC 7 cut(s) 657, 983, 1539, 1658, 2468, 2519, 2780
BcuI ACTAGT 1 cut(s) 668
BfmI CTRYAG 4 cut(s) 72, 499, 1299, 1488
BfrI CTTAAG 1 cut(s) 2014
BfuI GTATCC 2 cut(s) 710, 2290
BisI GCNGC 9 cut(s) 6, 75, 244, 1926, 1929, 1938, 1986, 2622, 2876
BlnI CCTAGG 1 cut(s) 2531
BlsI GCNGC 9 cut(s) 7, 76, 245, 1927, 1930, 1939, 1987, 2623, 2877
Bme1390I CCNGG 4 cut(s) 623, 2078, 2207, 2718
BmeT110I CYCGRG 1 cut(s) 1659
BmgBI CACGTC 1 cut(s) 1770
BmgT120I GGNCC 2 cut(s) 2002, 2633
BmiI GGNNCC 2 cut(s) 1605, 2715
BmrFI CCNGG 4 cut(s) 623, 2078, 2207, 2718
BmsI GCATC 7 cut(s) 103, 183, 1148, 1234, 1723, 1749, 1784
BmtI GCTAGC 1 cut(s) 1640
BpiI GAAGAC 1 cut(s) 2679
BplI GAGNNNNNCTC 2 cut(s) 1655, 1687
BpmI CTGGAG 3 cut(s) 1723, 2078, 2739
Bpu10I CCTNAGC 1 cut(s) 1053
BpuEI CTTGAG 4 cut(s) 169, 2610, 2804, 2825
BpuMI CCSGG 2 cut(s) 623, 2207
Bsa29I ATCGAT 1 cut(s) 2153
BsaAI YACGTR 1 cut(s) 833
BsaBI GATNNNNATC 1 cut(s) 1138
BsaHI GRCGYC 1 cut(s) 659
BsaJI CCNNGG 4 cut(s) 929, 966, 2531, 2692
BsaWI WCCGGW 1 cut(s) 1478
BsaXI ACNNNNNCTCC 6 cut(s) 1182, 1212, 1291, 1321, 1800, 1830
Bsc4I CCNNNNNNNGG 7 cut(s) 866, 1237, 1426, 1514, 1999, 2403, 2537
Bse1I ACTGG 7 cut(s) 208, 795, 997, 1806, 2061, 2397, 2644
Bse3DI GCAATG 4 cut(s) 313, 491, 1932, 1956
Bse8I GATNNNNATC 1 cut(s) 1138
BseBI CCWGG 2 cut(s) 2078, 2718
BseCI ATCGAT 1 cut(s) 2153
BseDI CCNNGG 4 cut(s) 929, 966, 2531, 2692
BseGI GGATG 7 cut(s) 304, 781, 1074, 1475, 1708, 1810, 2458
BseJI GATNNNNATC 1 cut(s) 1138
BseLI CCNNNNNNNGG 7 cut(s) 866, 1237, 1426, 1514, 1999, 2403, 2537
BseMI GCAATG 4 cut(s) 313, 491, 1932, 1956
BseMII CTCAG 9 cut(s) 765, 1012, 1044, 1245, 1550, 2028, 2073, 2265, 2769
BseNI ACTGG 7 cut(s) 208, 795, 997, 1806, 2061, 2397, 2644
BseRI GAGGAG 3 cut(s) 1299, 2506, 2557
BseXI GCAGC 8 cut(s) 17, 86, 255, 1915, 1924, 1972, 2608, 2862
BsgI GTGCAG 1 cut(s) 53
BshFI GGCC 7 cut(s) 850, 886, 934, 996, 1955, 2003, 2635
BshVI ATCGAT 1 cut(s) 2153
BsiHKAI GWGCWC 4 cut(s) 185, 1059, 1195, 2811
BsiHKCI CYCGRG 1 cut(s) 1659
BsiSI CCGG 4 cut(s) 622, 656, 1479, 2207
BslFI GGGAC 6 cut(s) 362, 525, 568, 882, 1132, 1139
BslI CCNNNNNNNGG 7 cut(s) 866, 1237, 1426, 1514, 1999, 2403, 2537
BsmAI GTCTC 7 cut(s) 657, 983, 1539, 1658, 2468, 2519, 2780
BsmFI GGGAC 6 cut(s) 362, 525, 568, 882, 1132, 1139
BsmI GAATGC 3 cut(s) 318, 1988, 2434
BsnI GGCC 7 cut(s) 850, 886, 934, 996, 1955, 2003, 2635
BsoBI CYCGRG 1 cut(s) 1659
Bsp1286I GDGCHC 4 cut(s) 185, 1059, 1195, 2811
Bsp1407I TGTACA 1 cut(s) 2346
Bsp143I GATC 8 cut(s) 232, 697, 1207, 1846, 1959, 2071, 2713, 2791
Bsp19I CCATGG 1 cut(s) 929
BspACI CCGC 5 cut(s) 146, 786, 913, 1926, 2887
BspANI GGCC 7 cut(s) 850, 886, 934, 996, 1955, 2003, 2635
BspCNI CTCAG 9 cut(s) 764, 1011, 1045, 1246, 1549, 2029, 2074, 2266, 2768
BspDI ATCGAT 1 cut(s) 2153
BspHI TCATGA 2 cut(s) 805, 2794
BspLI GGNNCC 2 cut(s) 1605, 2715
BspMAI CTGCAG 1 cut(s) 76
BspOI GCTAGC 1 cut(s) 1640
BspPI GGATC 5 cut(s) 240, 692, 1202, 2708, 2721
BspQI GCTCTTC 2 cut(s) 2022, 2360
BspTI CTTAAG 1 cut(s) 2014
BsrDI GCAATG 4 cut(s) 313, 491, 1932, 1956
BsrGI TGTACA 1 cut(s) 2346
BsrI ACTGG 7 cut(s) 208, 795, 997, 1806, 2061, 2397, 2644
BssECI CCNNGG 4 cut(s) 929, 966, 2531, 2692
BssMI GATC 8 cut(s) 232, 697, 1207, 1846, 1959, 2071, 2713, 2791
BssNI GRCGYC 1 cut(s) 659
BssT1I CCWWGG 3 cut(s) 929, 966, 2531
Bst2UI CCWGG 2 cut(s) 2078, 2718
Bst4CI ACNGT 9 cut(s) 120, 455, 467, 602, 722, 1013, 1446, 1699, 2221
Bst6I CTCTTC 7 cut(s) 1657, 1971, 2022, 2360, 2517, 2562, 2829
BstACI GRCGYC 1 cut(s) 659
BstAFI CTTAAG 1 cut(s) 2014
BstAPI GCANNNNNTGC 2 cut(s) 180, 2884
BstAUI TGTACA 1 cut(s) 2346
BstBAI YACGTR 1 cut(s) 833
BstC8I GCNNGC 4 cut(s) 893, 1593, 1638, 2034
BstDSI CCRYGG 2 cut(s) 929, 2692
BstENI CCTNNNNNAGG 1 cut(s) 2535
BstF5I GGATG 7 cut(s) 304, 781, 1074, 1475, 1708, 1810, 2458
BstKTI GATC 8 cut(s) 235, 700, 1210, 1849, 1962, 2074, 2716, 2794
BstMAI GTCTC 7 cut(s) 657, 983, 1539, 1658, 2468, 2519, 2780
BstMBI GATC 8 cut(s) 232, 697, 1207, 1846, 1959, 2071, 2713, 2791
BstMWI GCNNNNNNNGC 7 cut(s) 180, 592, 892, 1934, 1937, 1946, 2884
BstNI CCWGG 2 cut(s) 2078, 2718
BstNSI RCATGY 3 cut(s) 81, 566, 2815
BstSCI CCNGG 4 cut(s) 621, 2076, 2205, 2716
BstSFI CTRYAG 4 cut(s) 72, 499, 1299, 1488
BstV1I GCAGC 8 cut(s) 17, 86, 255, 1915, 1924, 1972, 2608, 2862
BstV2I GAAGAC 1 cut(s) 2679
BstX2I RGATCY 2 cut(s) 232, 2713
BstYI RGATCY 2 cut(s) 232, 2713
Bsu15I ATCGAT 1 cut(s) 2153
BsuI GTATCC 2 cut(s) 710, 2290
BsuRI GGCC 7 cut(s) 850, 886, 934, 996, 1955, 2003, 2635
BsuTUI ATCGAT 1 cut(s) 2153
BtgI CCRYGG 2 cut(s) 929, 2692
BtrI CACGTC 1 cut(s) 1770
BtsCI GGATG 7 cut(s) 304, 781, 1074, 1475, 1708, 1810, 2458
BtsI GCAGTG 2 cut(s) 1602, 2008
Cac8I GCNNGC 4 cut(s) 893, 1593, 1638, 2034
CciI TCATGA 2 cut(s) 805, 2794
Cfr13I GGNCC 2 cut(s) 2002, 2633
ClaI ATCGAT 1 cut(s) 2153
CseI GACGC 2 cut(s) 648, 884
Csp6I GTAC 4 cut(s) 26, 830, 1293, 2347
CviQI GTAC 4 cut(s) 26, 830, 1293, 2347
DpnI GATC 8 cut(s) 234, 699, 1209, 1848, 1961, 2073, 2715, 2793
DpnII GATC 8 cut(s) 232, 697, 1207, 1846, 1959, 2071, 2713, 2791
DraI TTTAAA 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 1953
Eam1104I CTCTTC 7 cut(s) 1657, 1971, 2022, 2360, 2517, 2562, 2829
EarI CTCTTC 7 cut(s) 1657, 1971, 2022, 2360, 2517, 2562, 2829
Ecl136II GAGCTC 1 cut(s) 1057
Eco130I CCWWGG 3 cut(s) 929, 966, 2531
Eco147I AGGCCT 1 cut(s) 886
Eco24I GRGCYC 1 cut(s) 1059
Eco32I GATATC 2 cut(s) 845, 2707
Eco53kI GAGCTC 1 cut(s) 1057
Eco57I CTGAAG 3 cut(s) 1187, 1419, 2859
Eco88I CYCGRG 1 cut(s) 1659
EcoICRI GAGCTC 1 cut(s) 1057
EcoNI CCTNNNNNAGG 1 cut(s) 2535
EcoO109I RGGNCCY 1 cut(s) 2002
EcoRII CCWGG 2 cut(s) 2076, 2716
EcoRV GATATC 2 cut(s) 845, 2707
EcoT14I CCWWGG 3 cut(s) 929, 966, 2531
EcoT22I ATGCAT 4 cut(s) 318, 372, 1141, 1886
EcoT38I GRGCYC 1 cut(s) 1059
ErhI CCWWGG 3 cut(s) 929, 966, 2531
FalI AAGNNNNNCTT 6 cut(s) 1296, 1328, 1552, 1584, 2123, 2155
FaqI GGGAC 6 cut(s) 362, 525, 568, 882, 1132, 1139
FauNDI CATATG 4 cut(s) 372, 1995, 2448, 2601
FbaI TGATCA 3 cut(s) 1846, 1959, 2791
FblI GTMKAC 1 cut(s) 2497
Fnu4HI GCNGC 9 cut(s) 6, 75, 244, 1926, 1929, 1938, 1986, 2622, 2876
FokI GGATG 7 cut(s) 311, 788, 1061, 1462, 1715, 1817, 2465
FriOI GRGCYC 1 cut(s) 1059
Fsp4HI GCNGC 9 cut(s) 6, 75, 244, 1926, 1929, 1938, 1986, 2622, 2876
GluI GCNGC 9 cut(s) 6, 75, 244, 1926, 1929, 1938, 1986, 2622, 2876
GsuI CTGGAG 3 cut(s) 1723, 2078, 2739
HaeIII GGCC 7 cut(s) 850, 886, 934, 996, 1955, 2003, 2635
HapII CCGG 4 cut(s) 622, 656, 1479, 2207
HgaI GACGC 2 cut(s) 648, 884
Hin1I GRCGYC 1 cut(s) 659
HincII GTYRAC 1 cut(s) 1016
HindII GTYRAC 1 cut(s) 1016
HindIII AAGCTT 2 cut(s) 1401, 2381
HpaII CCGG 4 cut(s) 622, 656, 1479, 2207
HphI GGTGA 5 cut(s) 331, 1077, 1727, 1844, 2666
Hpy166II GTNNAC 4 cut(s) 451, 463, 1016, 2498
Hpy8I GTNNAC 4 cut(s) 451, 463, 1016, 2498
Hpy99I CGWCG 1 cut(s) 678
HpyAV CCTTC 8 cut(s) 815, 876, 1050, 1594, 1823, 2015, 2080, 2834
HpyCH4III ACNGT 9 cut(s) 120, 455, 467, 602, 722, 1013, 1446, 1699, 2221
HpyCH4IV ACGT 4 cut(s) 589, 832, 1769, 2046
HpyF10VI GCNNNNNNNGC 7 cut(s) 180, 592, 892, 1934, 1937, 1946, 2884
HpySE526I ACGT 4 cut(s) 589, 832, 1769, 2046
Hsp92I GRCGYC 1 cut(s) 659
Ksp22I TGATCA 3 cut(s) 1846, 1959, 2791
Kzo9I GATC 8 cut(s) 232, 697, 1207, 1846, 1959, 2071, 2713, 2791
LguI GCTCTTC 2 cut(s) 2022, 2360
LmnI GCTCC 4 cut(s) 1190, 1312, 1974, 2834
Lsp1109I GCAGC 8 cut(s) 17, 86, 255, 1915, 1924, 1972, 2608, 2862
LweI GCATC 7 cut(s) 103, 183, 1148, 1234, 1723, 1749, 1784
MaeII ACGT 4 cut(s) 589, 832, 1769, 2046
MaeIII GTNAC 7 cut(s) 664, 915, 1083, 1353, 1440, 2251, 2291
MalI GATC 8 cut(s) 234, 699, 1209, 1848, 1961, 2073, 2715, 2793
MboI GATC 8 cut(s) 232, 697, 1207, 1846, 1959, 2071, 2713, 2791
MflI RGATCY 2 cut(s) 232, 2713
MhlI GDGCHC 4 cut(s) 185, 1059, 1195, 2811
MlsI TGGCCA 1 cut(s) 1955
MluNI TGGCCA 1 cut(s) 1955
MlyI GAGTC 7 cut(s) 743, 1265, 1643, 2470, 2503, 2521, 2554
MmeI TCCRAC 3 cut(s) 381, 1059, 2754
Mox20I TGGCCA 1 cut(s) 1955
Mph1103I ATGCAT 4 cut(s) 318, 372, 1141, 1886
MscI TGGCCA 1 cut(s) 1955
MslI CAYNNNNRTG 2 cut(s) 82, 2451
Msp20I TGGCCA 1 cut(s) 1955
MspA1I CMGCKG 2 cut(s) 1928, 2875
MspCI CTTAAG 1 cut(s) 2014
MspI CCGG 4 cut(s) 622, 656, 1479, 2207
MspR9I CCNGG 4 cut(s) 623, 2078, 2207, 2718
Mva1269I GAATGC 3 cut(s) 318, 1988, 2434
MvaI CCWGG 2 cut(s) 2078, 2718
MwoI GCNNNNNNNGC 7 cut(s) 180, 592, 892, 1934, 1937, 1946, 2884
NciI CCSGG 2 cut(s) 623, 2207
NcoI CCATGG 1 cut(s) 929
NdeI CATATG 4 cut(s) 372, 1995, 2448, 2601
NdeII GATC 8 cut(s) 232, 697, 1207, 1846, 1959, 2071, 2713, 2791
NheI GCTAGC 1 cut(s) 1636
NlaIV GGNNCC 2 cut(s) 1605, 2715
NmuCI GTSAC 5 cut(s) 664, 915, 1083, 1353, 1440
NsiI ATGCAT 4 cut(s) 318, 372, 1141, 1886
NspI RCATGY 3 cut(s) 81, 566, 2815
PagI TCATGA 2 cut(s) 805, 2794
PceI AGGCCT 1 cut(s) 886
PciSI GCTCTTC 2 cut(s) 2022, 2360
PcsI WCGNNNNNNNCGW 1 cut(s) 838
PctI GAATGC 3 cut(s) 318, 1988, 2434
PfeI GAWTC 8 cut(s) 1283, 1379, 1565, 1784, 1796, 2150, 2164, 2687
PflMI CCANNNNNTGG 2 cut(s) 866, 1514
PfoI TCCNGGA 1 cut(s) 2716
PkrI GCNGC 9 cut(s) 7, 76, 245, 1927, 1930, 1939, 1987, 2623, 2877
PleI GAGTC 7 cut(s) 743, 1264, 1643, 2470, 2502, 2521, 2553
PpsI GAGTC 7 cut(s) 743, 1264, 1643, 2470, 2502, 2521, 2553
Ppu21I YACGTR 1 cut(s) 833
PshBI ATTAAT 1 cut(s) 1113
PsiI TTATAA 3 cut(s) 978, 1220, 1409
Psp124BI GAGCTC 1 cut(s) 1059
Psp6I CCWGG 2 cut(s) 2076, 2716
PspGI CCWGG 2 cut(s) 2076, 2716
PspN4I GGNNCC 2 cut(s) 1605, 2715
PspPI GGNCC 2 cut(s) 2002, 2633
PsrI GAACNNNNNNTAC 2 cut(s) 199, 231
PstI CTGCAG 1 cut(s) 76
PsuI RGATCY 2 cut(s) 232, 2713
PvuII CAGCTG 1 cut(s) 2875
RsaI GTAC 4 cut(s) 27, 831, 1294, 2348
RsaNI GTAC 4 cut(s) 26, 830, 1293, 2347
RseI CAYNNNNRTG 2 cut(s) 82, 2451
SacI GAGCTC 1 cut(s) 1059
SapI GCTCTTC 2 cut(s) 2022, 2360
SatI GCNGC 9 cut(s) 6, 75, 244, 1926, 1929, 1938, 1986, 2622, 2876
Sau3AI GATC 8 cut(s) 232, 697, 1207, 1846, 1959, 2071, 2713, 2791
Sau96I GGNCC 2 cut(s) 2002, 2633
SchI GAGTC 7 cut(s) 743, 1265, 1643, 2470, 2503, 2521, 2554
ScrFI CCNGG 4 cut(s) 623, 2078, 2207, 2718
SduI GDGCHC 4 cut(s) 185, 1059, 1195, 2811
SfaNI GCATC 7 cut(s) 103, 183, 1148, 1234, 1723, 1749, 1784
SfcI CTRYAG 4 cut(s) 72, 499, 1299, 1488
SmiMI CAYNNNNRTG 2 cut(s) 82, 2451
SmlI CTYRAG 5 cut(s) 184, 2014, 2625, 2783, 2804
SmoI CTYRAG 5 cut(s) 184, 2014, 2625, 2783, 2804
SpeI ACTAGT 1 cut(s) 668
SseBI AGGCCT 1 cut(s) 886
SsiI CCGC 5 cut(s) 146, 786, 913, 1926, 2887
SspI AATATT 1 cut(s) 577
SstI GAGCTC 1 cut(s) 1059
StuI AGGCCT 1 cut(s) 886
StyD4I CCNGG 4 cut(s) 621, 2076, 2205, 2716
StyI CCWWGG 3 cut(s) 929, 966, 2531
TaaI ACNGT 9 cut(s) 120, 455, 467, 602, 722, 1013, 1446, 1699, 2221
TaiI ACGT 4 cut(s) 592, 835, 1772, 2049
TaqI TCGA 5 cut(s) 257, 616, 1369, 2153, 2857
TatI WGTACW 2 cut(s) 25, 2346
TauI GCSGC 1 cut(s) 1928
TfiI GAWTC 8 cut(s) 1283, 1379, 1565, 1784, 1796, 2150, 2164, 2687
TseFI GTSAC 5 cut(s) 664, 915, 1083, 1353, 1440
TseI GCWGC 8 cut(s) 5, 74, 243, 1928, 1937, 1985, 2621, 2875
Tsp45I GTSAC 5 cut(s) 664, 915, 1083, 1353, 1440
TspGWI ACGGA 1 cut(s) 1203
Van91I CCANNNNNTGG 2 cut(s) 866, 1514
Vha464I CTTAAG 1 cut(s) 2014
VspI ATTAAT 1 cut(s) 1113
XagI CCTNNNNNAGG 1 cut(s) 2535
XapI RAATTY 3 cut(s) 285, 983, 2123
XbaI TCTAGA 1 cut(s) 1096
XceI RCATGY 3 cut(s) 81, 566, 2815
XcmI CCANNNNNNNNNTGG 1 cut(s) 2074
XmaJI CCTAGG 1 cut(s) 2531
XmiI GTMKAC 1 cut(s) 2497
Zsp2I ATGCAT 4 cut(s) 318, 372, 1141, 1886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.