FvH4_2g23102

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005688
Physical Location & Seq
Reverse (-)
18953355 .. 18960976
7622 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23102.t2

Sequence Viewer

Length: 5388 bp
ATGGCGACTGTGCCCCAAGCAGATTCTAGACGCATGTATTCTTGGTGGTGGGACAGCCACATAAGCCCAAAAAACTCAAGATGGCTCAAGGAAAATCTTACAGATATGGATGCCAAAGTCAAACACATGATCAAACTGATTGAAGAAGATGCAGATTCCTTTGCTAGGAGGGCAGAAATGTATTACAAGAAGCGGCCAGAACTCATGAAATTGGTTGAAGAGTTCTACCGAGCATACCGTGCGTTAGCTGAAAGATATGACCATGCAACTGGGGCGCTGCGCCAGGCTCATCGAACGATGGCAGAGGCGTTTCCTAACCAAGTCCCGTTCGTAGATGACTCACCTGCAGGTTCCTCTGCTAGTGAGACTGATCCCCGAACACCTGAGATGCCAGCTCCTATACGAGCATTATTTGACTTTGATGAATTACAAAAAGATGCTCTGGGACTGTCCTCCTCAACCCATTTCCATGCTCTCAAAAGGAATGGAGCTTTTAGTGAAGAATCTGACTCTGGAACCAGCCGAATAGGTTTGAAACAGCTGAATGATCTCTTTGGATCGGGAGAAGGAAGGGCGAAAAGGGGCCTCAACTTCCTTGATGCTGAAGCAAAAGAACACAGCATGCAGAACAATGGCCATGATCTCAAGACTCGAGCCTTGTTGGAAAATGATCGAGTGGGCAAAGCAGAGACAGAAATATCAAACTTAAAGAAAGCTCTTGCTAAACTAGAAGCTGAAAAGGAAGCTGGCTTACTTCAGTACCAAGAGTGTTTAGAGAGATTATCTAATTTGGAGTCTGAAGTCTCTCGTGCACAAGAGGATTCCAGGGGACTCAATGAACGAGCCAGTGAAGCTGAAGCTGAAGTTCAAACCACAAAGGAAGCACTTAACAAATTAGAGGCTGAAAGGGAAGCTAGTCTTCTTCAGTATCAAGAGTGCTTAGACAAAATCTCTAACCTGGAAAACATCATCTCTTGTGCCCAAAAGGATGCTGGAGAGCTCAATGATCGAGCTAGCAAAGCTGAATTTGCATCTGAATCACTACAGAAAGATCTTGAAAGGGTGGCATCTGAGAAAGAAGCTGCACTTGTACAATACAAACAATGTCTGGAGAAGATATCGAATCTGGAGGAGAAACTACTGGATGTTGAGGAGGAGGCCAAGAGGGCTAATGAGCGTGCTGTAATAGCTGAATGTGAAGTTGAATCCTTGAAGCAAGCAGTTGCCAATTTAACTGAAGAGAAGGAAGCTGCTGCTCTCCAGTACAAGCAATGCCTGGAAACGATATCTAATCTTGAGCATAAGATCTCTCGTGCCGAGGAGGAAGCGCTACGGCTACACTCTCAGATAGATGATGGGATTGCGAAGTTAAAGGATTCGGAAGAAAAGTGTCTTCTGCTGGTAAACTCAAATCAGAATCTGCAGTCGGAGTTGGAGTCTGCAGTGAAGCAAATGCAATCTCAAGGTGAAGAACTTACAGAGAAGCAGAAAGAGTTGGGTAGGCTTTGGGCTTGCATACAAGAAGAGCGGTTACGATTTTTGGAGGCTGAAACTGCTTTCCAAACACTGCAGCATTTACATTCCCAATCCCAGGAGGAACTTAGATCTCTCGTAGCTGAGCTCCAGAATAGAAATCTGATTCTAAAGGACATGGAAGCACGAAGTCAGAGTTTGGATAATGAAGTCCAGAAGGTGAAGGAGGAAAACAAGAGCCTAAGTGAGATCAATTTGTCTTCATCTATTTCAATAAAAGATCTGCAAGATGAGATCTTAATCTTGAGGGAAACGATTAAGAAACTTGAAGAGGAAGTTGAACTTCGAGTGGACCAAAGAAATGCTCTTCAGCAAGAGATATACTGTCTGAAAGAGGAACTCAGTGACTTGAACAAGAAACACCAAGCTATGCTAGAGCAGGTGGACTCAGTTGGCATGGATCCAGTGTGCATTGGGTCATCTGTGAAGGAGATGCAAGATGAAAACTTACAGCTCAAACAAACGTGTGAGGCAGAGAAAAGTGAGAAAGTGGCACTGTTGGAAAAGTTGGAAATCATGCAGAAACTTCAGGAGAAAAATGTTCTTTTGGAGAACTCTCTTTCTGATTTGAATGTTGAATTAGAAGGGGTTAGAGGGAAGGTAAAGGATTTAGAACAATCATGTCAGTCTCTTCTGGCAGAAAAAGGTACTCTTCTTGCTGAAAATGGCACCCTGATTTACCAGTTGCAGATTGTTACTGAGAATTTAGATAAATCATTAGAGAAGAACAACTTTTTGGAGAATTCTCTCTTTGATGCCAATGCTGAACTGGAAGGCTTGAGTGTAAAATCAAAGAGCTTAGAAGAATCATGCCTATTGCTTGGTAATGAGAAGACTGGTCTGATCACTGAGAGAGAGAGTTTAATTTTGAAGTTGGGCAGTACTCGTTCAAGACTTGAAGATCTGGAGAAAGGATACGCAGAGATAGAAGAGAAGCTCTCTGTTCTGAAGAAGGAAAGAGATTCCGCACTTTGTAAAGTGGAAGAACTAAATGTATGCTTAGATTCTGAGAAGCAAAATCATGCCAGTTCTGTTGAGTTAAGGGAAACCCAGTTGGCTGATATGGAACTGAAGATCTCTGGTCTTGAAGCAGAGGGTATATGTAGGAAGAAAGAATTTGAAGAGGAACAAGACAAATCTGTGACTGCACAGATTGAAATCTTTGTCCTGCAGAAATGTGTAGAAGATCTGGAAGAAAAGAACTTATCCCTCATGATTGAGCGTCAAAAGCTTTTGGGGGCATCCACAATGTCAGAGAAGCTGATTTCTGTCCTTGAGCGTGGCAAACTTGAACAACAGAGGGAGATTAAGTCATTGTTTGTGCAACTTAAAGCACTGAGAATGGGGCTGTATCAGGTGTTGAAGACTGTTGACATTGATGCAAACCTGGGGTGTGCAGAAAAGGATGACCAAGACCAATCACTCCTTAACCATATACTTGTCAAACTTCAGGACAAACAAAATTCTTTCGCTGAAAGTTGTGATGAAAACCAGCAGTTGCTTATTGAGAACTCTGTTCTCGTAGCAATGCTTGCCCAACTGAAACTAGAGGCGGACTGTTTTATGAGGGAAAGAGACACTCTTGACCATGAGTTCCGGACCCAGTCTGAAAAGTTCTTGGTGTTGCAGAGTGGCGCCCAAAGACTTCACGACATGAATGAAGAATTGAATTTGAAAGTAGTGGAGGGAGAACACAGAGAGGGAGTATTGAGGACCGAAATAGATAATCTGCACGAGCAGTTGTTGGACTTGCAAAGCGTTTACAGAAGTCTACAAAAAGAAAATTGCCAGGTGGTTGAATATAAAGGATCCTTGAAAAAGACGGTCTTAAATTTGGAGGAAGAGACACGTAACCTAGAGGAGGATAAATGTGTTATGTTTGCTGAAACAATATATTATAGTAACCTCTCCCTGGTTTTCGATGATATTATTTCCCAAAAGCAACTGGAACTGGAAGAGCTCAGTCACAATTATGATGAGCTTCATCTTGGCAATAATGACCTGAAAGCGAAGGTAAGAATATTGGAGGGGCAGTTGGAAGTTATACAGATGGAAAACTTACATCTCAAGGAGTCATTAAGTAAGTCAGAGGATGAGCTGAAATTGGTTAAATCTGTGAATGATCAATTGAATGGTGATATTGCAAATGCAAAGGATGGTTTGTCTCAAAAGGAGATTGAGCTTTTGGTAGCCGGGCAGATCATCAATGAGCTACATAATGAGAAACAAGAGCTGTATGTACTGGTGGAGGATTTGACTGCTAAAAGTGATGATGCGAAGATGGTGCTGGAAGATCAAGAGAAGAAAATTCTCAAGCTACATGAAGACAGTGACCTTCATAGTAAAGAGATTGGATGCCTCCGTGAAGTGAATCAGAAGTTGGAGGTGGAACTGTCAAAATCGCATGAAGAAGCTGAAAAGGCAAAAATTGAGGAGGAAAGGTTGATCAGTGAACTAAAAGCGGGAAGAGAAGAGATTGAAATGTGGGTGGCTCAGGCTGCCACCCTCTTCAGAGAACTGCAGATCTCCTCCATTCGTGAAACGTTATTTGAAGGAAAGATCCGTGAGCTCATTGAAGCCTATCAGATCCTTGAAGAGAAAAGCATTTCCAAGGCCCTGGAGAATGAACAAATGAAAGAAAGAGTTGGCACATTGGAGCATGAAAATGGAGAGCTACAAGCTCAGTTGGCTGCCTATATCCCAGCTGTCATCTCTCTGAAGGAGTGTACAACAGCCCTTGAGAATCATTCGCTTATTACCACCACATCTCATAAACTTGACATTGGAGCCTTGGAGGATGCCCTTATGCAAGCTGAGAGGTCTCAAACAGATGGACATCAAATTGACACAGTATCAGATGGCATTTCAGAATTACAGGATTTACAAAGAAGAATCAAAGCTATAGAAAAGGCCATGGTAGAAAAGGAAAGCCATCTTGTTGCCAATGAGGAAGCTAAACGATTTGGGGACGGCAAGAAGCCCGAAATCTCTGAATCAGGGAATGAAGTTCTGACAAAAGACATCATTCTGGATCAGATATCAGAGTGTTCATCCTATGGGGTAAGCAGGAGAGAAACCGCAGAGCCTGATCCTCAGATTCTGGAGTTATGGAAGACCACTGACCAGGATGGCAGCATTGATTTGATGGTTGGCAAGGCCCAGAAGGCAACTACTGTACCGACTGACCACAGTCAGACTGAAGCAATAAAGAAACACAAGAACAAATATCCCTCATCAGAATCATTGGTTGAGAAAGAATATAGCATTGACAAATTAGAGATCTCGAAGAGATTCTCTGAACCCCGTCAAGAAGGCAACAAGAGAAAGATTCTGGAAAGACTTGATTCTGATGTGCAAAAGTTGACAAACCTTCAGATTACCGTGGAAGACCTGAAGAAGAAGGTGGAAATTACTGAGAGGACCAAAAAGGGAAAAGGTATTGAATTTGGTACTGTTAGAGAGCAGCTGGATGAAGCTGAAGAAGCCATCACTAGGTTGTTTGATGCCAATAACAAATTGATGAAGAGTGTGGAAGATGATTTTGTGTCTCCTCCTAATGGAGATTCTGGGATTGTCCCGGATCACAGTGGGAGTGTCAGCAGAAAGAGACTTTCGGAACAAGCAAAAAGAGGGTCAGAGAAAATCGGTCGACTGCAGTTGGAGGTACAGAAACTGCAATTTCTTCTACTGAAGCTCGACGGTGAAAAGGAAAGCAAAGGGTCAACAAGGATCAAGGAGAGGAAGACAAGAGTTCTTCTGCGGGACTATCTCTATTCTGGCAGAACAACCGCCACAACCCCAAAGCGTAAGAAGGCTCCTTTTTGTGCATGCATGCCCCCAACAAAGGGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIP1

Protein Analysis

1796

Amino Acids

204.02

Weight (kDa)

4.97

Isoelectric Point (pI)

51.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 14 - 87 1.7e-37 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 352, 1903
Acc36I ACCTGC 3 cut(s) 338, 352, 1903
AccB1I GGYRCC 2 cut(s) 2201, 3167
AccBSI CCGCTC 1 cut(s) 1528
AccI GTMKAC 2 cut(s) 3304, 5182
AccIII TCCGGA 1 cut(s) 3129
AciI CCGC 8 cut(s) 193, 1528, 2499, 3086, 3995, 4611, 5293, 5322
AclI AACGTT 1 cut(s) 4076
AcoI YGGCCR 2 cut(s) 194, 634
AcsI RAATTY 9 cut(s) 1025, 2236, 2275, 2648, 2995, 3202, 3364, 3840, 4976
AcyI GRCGYC 1 cut(s) 3168
AfeI AGCGCT 1 cut(s) 1329
AfiI CCNNNNNNNGG 8 cut(s) 165, 1591, 3394, 3445, 5025, 5090, 5377, 5378
AflIII ACRYGT 2 cut(s) 1997, 3380
AjuI GAANNNNNNNTTGG 4 cut(s) 2252, 2284, 3896, 3928
Alw21I GWGCWC 5 cut(s) 814, 1002, 1623, 3495, 4104
Alw44I GTGCAC 1 cut(s) 810
AlwNI CAGNNNCTG 4 cut(s) 1420, 4619, 4633, 5206
Ama87I CYCGRG 1 cut(s) 651
Aor13HI TCCGGA 1 cut(s) 3129
Aor51HI AGCGCT 1 cut(s) 1329
AoxI GGCC 7 cut(s) 194, 583, 634, 1158, 4146, 4443, 4689
ApaLI GTGCAC 1 cut(s) 810
ApeKI GCWGC 9 cut(s) 277, 1082, 1250, 1253, 1570, 4031, 4223, 4665, 4996
ApoI RAATTY 9 cut(s) 1025, 2236, 2275, 2648, 2995, 3202, 3364, 3840, 4976
ArsI GACNNNNNNTTYG 2 cut(s) 5150, 5182
Asp700I GAANNNNTTC 1 cut(s) 4823
AspLEI GCGC 4 cut(s) 277, 282, 1330, 3170
AspS9I GGNCC 7 cut(s) 583, 1825, 3132, 3246, 4147, 4690, 4953
AsuC2I CCSGG 2 cut(s) 3727, 5111
AsuHPI GGTGA 5 cut(s) 333, 1478, 1705, 3680, 5246
AsuNHI GCTAGC 1 cut(s) 1013
AvaI CYCGRG 1 cut(s) 651
AvaII GGWCC 4 cut(s) 1825, 3132, 3246, 4953
BaeGI GKGCMC 3 cut(s) 15, 814, 982
BaeI ACNNNNGTAYC 4 cut(s) 743, 776, 4692, 4725
BalI TGGCCA 1 cut(s) 636
BamHI GGATCC 2 cut(s) 1933, 3341
BanI GGYRCC 2 cut(s) 2201, 3167
BanII GRGCYC 4 cut(s) 1002, 1623, 3495, 4104
BarI GAAGNNNNNNTAC 4 cut(s) 735, 767, 1515, 1547
BauI CACGAG 3 cut(s) 807, 1311, 3266
BbsI GAAGAC 9 cut(s) 911, 1385, 1725, 2372, 2903, 3864, 4652, 4926, 5282
Bbv12I GWGCWC 5 cut(s) 814, 1002, 1623, 3495, 4104
BbvI GCAGC 9 cut(s) 264, 1069, 1237, 1240, 1582, 4018, 4210, 4677, 5008
BceAI ACGGC 2 cut(s) 1349, 4519
BcgI CGANNNNNNTGC 2 cut(s) 3799, 3833
BciVI GTATCC 1 cut(s) 2441
BclI TGATCA 4 cut(s) 129, 2376, 3655, 3978
BcnI CCSGG 2 cut(s) 3727, 5111
BfmI CTRYAG 9 cut(s) 345, 1043, 1421, 1440, 1568, 2702, 4052, 4434, 5186
BfoI RGCGCY 3 cut(s) 278, 1331, 3171
BfuAI ACCTGC 3 cut(s) 338, 352, 1903
BfuI GTATCC 1 cut(s) 2441
BglI GCCNNNNNGGC 2 cut(s) 1166, 4697
BlpI GCTNAGC 1 cut(s) 1617
BmcAI AGTACT 1 cut(s) 2416
Bme18I GGWCC 4 cut(s) 1825, 3132, 3246, 4953
BmeT110I CYCGRG 1 cut(s) 651
BmgT120I GGNCC 7 cut(s) 583, 1825, 3132, 3246, 4147, 4690, 4953
BmrI ACTGGG 3 cut(s) 279, 2578, 3130
BmtI GCTAGC 1 cut(s) 1017
BmuI ACTGGG 3 cut(s) 279, 2578, 3130
BoxI GACNNNNGTC 1 cut(s) 4722
BpiI GAAGAC 9 cut(s) 911, 1385, 1725, 2372, 2903, 3864, 4652, 4926, 5282
BplI GAGNNNNNCTC 2 cut(s) 2456, 2488
BpmI CTGGAG 8 cut(s) 1014, 1130, 1148, 1244, 1607, 2459, 4172, 4655
Bpu10I CCTNAGC 1 cut(s) 4026
Bpu1102I GCTNAGC 1 cut(s) 1617
BpuMI CCSGG 2 cut(s) 3727, 5111
BsaAI YACGTR 1 cut(s) 3383
BsaBI GATNNNNATC 3 cut(s) 1772, 2690, 4368
BsaHI GRCGYC 1 cut(s) 3168
BsaI GGTCTC 1 cut(s) 4359
BsaWI WCCGGW 1 cut(s) 3129
BsaXI ACNNNNNCTCC 8 cut(s) 1421, 1451, 2828, 2858, 3551, 3581, 3959, 3989
Bsc4I CCNNNNNNNGG 8 cut(s) 165, 1591, 3394, 3445, 5025, 5090, 5377, 5378
Bse3DI GCAATG 2 cut(s) 1277, 3066
Bse8I GATNNNNATC 3 cut(s) 1772, 2690, 4368
BseAI TCCGGA 1 cut(s) 3129
BseJI GATNNNNATC 3 cut(s) 1772, 2690, 4368
BseLI CCNNNNNNNGG 8 cut(s) 165, 1591, 3394, 3445, 5025, 5090, 5377, 5378
BseMI GCAATG 2 cut(s) 1277, 3066
BseRI GAGGAG 9 cut(s) 445, 1145, 1166, 1169, 1334, 3407, 3980, 4051, 5073
BseSI GKGCMC 3 cut(s) 15, 814, 982
BseXI GCAGC 9 cut(s) 264, 1069, 1237, 1240, 1582, 4018, 4210, 4677, 5008
BseYI CCCAGC 1 cut(s) 4234
BsgI GTGCAG 4 cut(s) 1068, 2664, 2949, 3248
Bsh1285I CGRYCG 1 cut(s) 5182
BshFI GGCC 7 cut(s) 196, 585, 636, 1160, 4148, 4445, 4691
BshNI GGYRCC 2 cut(s) 2201, 3167
BsiEI CGRYCG 1 cut(s) 5182
BsiHKAI GWGCWC 5 cut(s) 814, 1002, 1623, 3495, 4104
BsiHKCI CYCGRG 1 cut(s) 651
BsiSI CCGG 3 cut(s) 3130, 3726, 5111
BslFI GGGAC 7 cut(s) 65, 308, 459, 843, 4514, 5093, 5309
BslI CCNNNNNNNGG 8 cut(s) 165, 1591, 3394, 3445, 5025, 5090, 5377, 5378
BsmFI GGGAC 7 cut(s) 65, 308, 459, 843, 4514, 5093, 5309
BsnI GGCC 7 cut(s) 196, 585, 636, 1160, 4148, 4445, 4691
Bso31I GGTCTC 1 cut(s) 4359
BsoBI CYCGRG 1 cut(s) 651
Bsp1286I GDGCHC 7 cut(s) 15, 814, 982, 1002, 1623, 3495, 4104
Bsp13I TCCGGA 1 cut(s) 3129
Bsp1407I TGTACA 2 cut(s) 1090, 4259
Bsp1720I GCTNAGC 1 cut(s) 1617
Bsp19I CCATGG 1 cut(s) 4446
BspACI CCGC 8 cut(s) 193, 1528, 2499, 3086, 3995, 4611, 5293, 5322
BspANI GGCC 7 cut(s) 196, 585, 636, 1160, 4148, 4445, 4691
BspEI TCCGGA 1 cut(s) 3129
BspHI TCATGA 2 cut(s) 204, 2745
BspMAI CTGCAG 7 cut(s) 349, 1425, 1444, 1572, 2706, 4056, 5190
BspMI ACCTGC 3 cut(s) 338, 352, 1903
BspOI GCTAGC 1 cut(s) 1017
BspQI GCTCTTC 3 cut(s) 1518, 1845, 3483
BspT107I GGYRCC 2 cut(s) 2201, 3167
BspTNI GGTCTC 1 cut(s) 4359
BsrBI CCGCTC 1 cut(s) 1528
BsrDI GCAATG 2 cut(s) 1277, 3066
BsrGI TGTACA 2 cut(s) 1090, 4259
BssNI GRCGYC 1 cut(s) 3168
BssSI CACGAG 3 cut(s) 807, 1311, 3266
BssT1I CCWWGG 3 cut(s) 4143, 4323, 4446
Bst2BI CACGAG 3 cut(s) 807, 1311, 3266
BstACI GRCGYC 1 cut(s) 3168
BstAPI GCANNNNNTGC 2 cut(s) 239, 3065
BstAUI TGTACA 2 cut(s) 1090, 4259
BstBAI YACGTR 1 cut(s) 3383
BstDSI CCRYGG 2 cut(s) 4446, 4914
BstENI CCTNNNNNAGG 2 cut(s) 163, 3392
BstH2I RGCGCY 3 cut(s) 278, 1331, 3171
BstHHI GCGC 4 cut(s) 277, 282, 1330, 3170
BstMCI CGRYCG 1 cut(s) 5182
BstNSI RCATGY 4 cut(s) 37, 625, 5364, 5368
BstPAI GACNNNNGTC 1 cut(s) 4722
BstSFI CTRYAG 9 cut(s) 345, 1043, 1421, 1440, 1568, 2702, 4052, 4434, 5186
BstSLI GKGCMC 3 cut(s) 15, 814, 982
BstV1I GCAGC 9 cut(s) 264, 1069, 1237, 1240, 1582, 4018, 4210, 4677, 5008
BstV2I GAAGAC 9 cut(s) 911, 1385, 1725, 2372, 2903, 3864, 4652, 4926, 5282
BstXI CCANNNNNNTGG 2 cut(s) 269, 4150
BsuI GTATCC 1 cut(s) 2441
BsuRI GGCC 7 cut(s) 196, 585, 636, 1160, 4148, 4445, 4691
BtgI CCRYGG 2 cut(s) 4446, 4914
BtsI GCAGTG 2 cut(s) 1449, 1565
BveI ACCTGC 3 cut(s) 338, 352, 1903
CaiI CAGNNNCTG 4 cut(s) 1420, 4619, 4633, 5206
CciI TCATGA 2 cut(s) 204, 2745
CfoI GCGC 4 cut(s) 277, 282, 1330, 3170
Cfr13I GGNCC 7 cut(s) 583, 1825, 3132, 3246, 4147, 4690, 4953
CseI GACGC 2 cut(s) 39, 2744
DinI GGCGCC 1 cut(s) 3169
EaeI YGGCCR 2 cut(s) 194, 634
EciI GGCGGA 1 cut(s) 3101
Ecl136II GAGCTC 4 cut(s) 1000, 1621, 3493, 4102
Eco130I CCWWGG 3 cut(s) 4143, 4323, 4446
Eco24I GRGCYC 4 cut(s) 1002, 1623, 3495, 4104
Eco31I GGTCTC 1 cut(s) 4359
Eco32I GATATC 3 cut(s) 1119, 1287, 4572
Eco47I GGWCC 4 cut(s) 1825, 3132, 3246, 4953
Eco47III AGCGCT 1 cut(s) 1329
Eco53kI GAGCTC 4 cut(s) 1000, 1621, 3493, 4102
Eco88I CYCGRG 1 cut(s) 651
EcoICRI GAGCTC 4 cut(s) 1000, 1621, 3493, 4102
EcoNI CCTNNNNNAGG 2 cut(s) 163, 3392
EcoO109I RGGNCCY 2 cut(s) 583, 4147
EcoRI GAATTC 1 cut(s) 2275
EcoRV GATATC 3 cut(s) 1119, 1287, 4572
EcoT14I CCWWGG 3 cut(s) 4143, 4323, 4446
EcoT22I ATGCAT 1 cut(s) 5366
EcoT38I GRGCYC 4 cut(s) 1002, 1623, 3495, 4104
EgeI GGCGCC 1 cut(s) 3169
EheI GGCGCC 1 cut(s) 3169
ErhI CCWWGG 3 cut(s) 4143, 4323, 4446
FaqI GGGAC 7 cut(s) 65, 308, 459, 843, 4514, 5093, 5309
FauI CCCGC 2 cut(s) 3988, 5286
FbaI TGATCA 4 cut(s) 129, 2376, 3655, 3978
FblI GTMKAC 2 cut(s) 3304, 5182
FriOI GRGCYC 4 cut(s) 1002, 1623, 3495, 4104
GlaI GCGC 4 cut(s) 276, 281, 1329, 3169
GsaI CCCAGC 1 cut(s) 4238
GsuI CTGGAG 8 cut(s) 1014, 1130, 1148, 1244, 1607, 2459, 4172, 4655
HaeII RGCGCY 3 cut(s) 278, 1331, 3171
HaeIII GGCC 7 cut(s) 196, 585, 636, 1160, 4148, 4445, 4691
HapII CCGG 3 cut(s) 3130, 3726, 5111
HgaI GACGC 2 cut(s) 39, 2744
HhaI GCGC 4 cut(s) 277, 282, 1330, 3170
Hin1I GRCGYC 1 cut(s) 3168
Hin6I GCGC 4 cut(s) 275, 280, 1328, 3168
HinP1I GCGC 4 cut(s) 275, 280, 1328, 3168
HincII GTYRAC 4 cut(s) 2905, 4896, 5183, 5256
HindII GTYRAC 4 cut(s) 2905, 4896, 5183, 5256
HindIII AAGCTT 1 cut(s) 2762
HpaII CCGG 3 cut(s) 3130, 3726, 5111
HphI GGTGA 5 cut(s) 333, 1478, 1705, 3680, 5246
Hpy99I CGWCG 1 cut(s) 5234
HpyCH4IV ACGT 3 cut(s) 1997, 3382, 4076
HpySE526I ACGT 3 cut(s) 1997, 3382, 4076
Hsp92I GRCGYC 1 cut(s) 3168
HspAI GCGC 4 cut(s) 275, 280, 1328, 3168
KasI GGCGCC 1 cut(s) 3167
Kpn2I TCCGGA 1 cut(s) 3129
Ksp22I TGATCA 4 cut(s) 129, 2376, 3655, 3978
LguI GCTCTTC 3 cut(s) 1518, 1845, 3483
LmnI GCTCC 6 cut(s) 400, 488, 1626, 4189, 4319, 5353
Lsp1109I GCAGC 9 cut(s) 264, 1069, 1237, 1240, 1582, 4018, 4210, 4677, 5008
MaeII ACGT 3 cut(s) 1997, 3382, 4076
MaeIII GTNAC 8 cut(s) 1530, 1877, 2227, 2674, 3383, 3434, 3497, 3863
MbiI CCGCTC 1 cut(s) 1528
MfeI CAATTG 1 cut(s) 3659
MhlI GDGCHC 7 cut(s) 15, 814, 982, 1002, 1623, 3495, 4104
MlsI TGGCCA 1 cut(s) 636
MluNI TGGCCA 1 cut(s) 636
Mly113I GGCGCC 1 cut(s) 3168
MlyI GAGTC 8 cut(s) 332, 503, 643, 803, 825, 1445, 1913, 3614
MmeI TCCRAC 9 cut(s) 642, 1407, 1413, 2013, 2022, 3258, 3549, 3894, 5172
Mox20I TGGCCA 1 cut(s) 636
Mph1103I ATGCAT 1 cut(s) 5366
MroI TCCGGA 1 cut(s) 3129
MroXI GAANNNNTTC 1 cut(s) 4823
MscI TGGCCA 1 cut(s) 636
MslI CAYNNNNRTG 3 cut(s) 468, 3504, 4197
Msp20I TGGCCA 1 cut(s) 636
MspA1I CMGCKG 3 cut(s) 541, 4238, 4999
MspI CCGG 3 cut(s) 3130, 3726, 5111
MteI GCGCNGCGC 1 cut(s) 278
MunI CAATTG 1 cut(s) 3659
NarI GGCGCC 1 cut(s) 3168
NciI CCSGG 2 cut(s) 3727, 5111
NcoI CCATGG 1 cut(s) 4446
NheI GCTAGC 1 cut(s) 1013
NmeAIII GCCGAG 1 cut(s) 1342
NmuCI GTSAC 4 cut(s) 1877, 2674, 3497, 3863
NsiI ATGCAT 1 cut(s) 5366
NspI RCATGY 4 cut(s) 37, 625, 5364, 5368
PaeI GCATGC 3 cut(s) 625, 5364, 5368
PaeR7I CTCGAG 1 cut(s) 651
PagI TCATGA 2 cut(s) 204, 2745
PaqCI CACCTGC 2 cut(s) 352, 1903
PciSI GCTCTTC 3 cut(s) 1518, 1845, 3483
PdmI GAANNNNTTC 1 cut(s) 4823
PflFI GACNNNGTC 1 cut(s) 3136
PfoI TCCNGGA 1 cut(s) 5109
PleI GAGTC 8 cut(s) 332, 503, 643, 802, 825, 1444, 1913, 3613
PluTI GGCGCC 1 cut(s) 3171
PpsI GAGTC 8 cut(s) 332, 503, 643, 802, 825, 1444, 1913, 3613
Ppu21I YACGTR 1 cut(s) 3383
PshAI GACNNNNGTC 1 cut(s) 4722
Psp124BI GAGCTC 4 cut(s) 1002, 1623, 3495, 4104
Psp1406I AACGTT 1 cut(s) 4076
PspFI CCCAGC 1 cut(s) 4234
PspPI GGNCC 7 cut(s) 583, 1825, 3132, 3246, 4147, 4690, 4953
PspXI VCTCGAGB 1 cut(s) 651
PstI CTGCAG 7 cut(s) 349, 1425, 1444, 1572, 2706, 4056, 5190
PstNI CAGNNNCTG 4 cut(s) 1420, 4619, 4633, 5206
PsyI GACNNNGTC 1 cut(s) 3136
PvuII CAGCTG 3 cut(s) 541, 4238, 4999
RseI CAYNNNNRTG 3 cut(s) 468, 3504, 4197
SacI GAGCTC 4 cut(s) 1002, 1623, 3495, 4104
SalI GTCGAC 1 cut(s) 5181
SapI GCTCTTC 3 cut(s) 1518, 1845, 3483
Sau96I GGNCC 7 cut(s) 583, 1825, 3132, 3246, 4147, 4690, 4953
SbfI CCTGCAGG 1 cut(s) 349
ScaI AGTACT 1 cut(s) 2416
SchI GAGTC 8 cut(s) 332, 503, 643, 803, 825, 1445, 1913, 3614
SdaI CCTGCAGG 1 cut(s) 349
SduI GDGCHC 7 cut(s) 15, 814, 982, 1002, 1623, 3495, 4104
SfcI CTRYAG 9 cut(s) 345, 1043, 1421, 1440, 1568, 2702, 4052, 4434, 5186
SfoI GGCGCC 1 cut(s) 3169
Sfr274I CTCGAG 1 cut(s) 651
SinI GGWCC 4 cut(s) 1825, 3132, 3246, 4953
SlaI CTCGAG 1 cut(s) 651
SmiMI CAYNNNNRTG 3 cut(s) 468, 3504, 4197
SphI GCATGC 3 cut(s) 625, 5364, 5368
Sse8387I CCTGCAGG 1 cut(s) 349
SsiI CCGC 8 cut(s) 193, 1528, 2499, 3086, 3995, 4611, 5293, 5322
SspDI GGCGCC 1 cut(s) 3167
SspI AATATT 1 cut(s) 3555
SstI GAGCTC 4 cut(s) 1002, 1623, 3495, 4104
StyI CCWWGG 3 cut(s) 4143, 4323, 4446
TaiI ACGT 3 cut(s) 2000, 3385, 4079
TaqII GACCGA 2 cut(s) 3263, 5168
TatI WGTACW 5 cut(s) 1090, 1263, 2414, 3772, 4259
TauI GCSGC 1 cut(s) 196
TseFI GTSAC 4 cut(s) 1877, 2674, 3497, 3863
TseI GCWGC 9 cut(s) 277, 1082, 1250, 1253, 1570, 4031, 4223, 4665, 4996
Tsp45I GTSAC 4 cut(s) 1877, 2674, 3497, 3863
TspGWI ACGGA 2 cut(s) 3884, 4085
Tth111I GACNNNGTC 1 cut(s) 3136
VneI GTGCAC 1 cut(s) 810
VpaK11BI GGWCC 4 cut(s) 1825, 3132, 3246, 4953
XagI CCTNNNNNAGG 2 cut(s) 163, 3392
XapI RAATTY 9 cut(s) 1025, 2236, 2275, 2648, 2995, 3202, 3364, 3840, 4976
XbaI TCTAGA 1 cut(s) 26
XceI RCATGY 4 cut(s) 37, 625, 5364, 5368
XcmI CCANNNNNNNNNTGG 2 cut(s) 989, 2299
XhoI CTCGAG 1 cut(s) 651
XmiI GTMKAC 2 cut(s) 3304, 5182
XmnI GAANNNNTTC 1 cut(s) 4823
ZrmI AGTACT 1 cut(s) 2416
Zsp2I ATGCAT 1 cut(s) 5366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.