MD11G1280400.v1.1

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005688
Physical Location & Seq
Reverse (-)
39810187 .. 39817586
7400 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1280400.v1.1.491

Sequence Viewer

Length: 5376 bp
ATGACTACCGCGTCGCAGGCAGACTCTAGACGCAAGTATTCTTGGTGGTGGGATAGCCACATAAGCCCAAAGAATTCGAAATGGCTTCAGGAAAATCTTACAGATATGGATGTCAAAGTCAAACACATGATCAAGCTCATTGAATTGGATGCTGATTCCTTTGCTAGGAGGGCCGAGATGTACTATAAACAGCGCCCAGAATTAATGAAATTGGTTGAAGAGTTCTATCGAGCATACCGTGCGTTAGCTGAAAGATATGATCATGCAACTGGGGCGCTGCGCCAGGCTCATCGAACCATGGCAGAAGCATTTCCCAACCAAGTTCCCTTTGCAATGGGAGATGATTCACCGGCAGGTTCCTGTGCTAGTGAGGCTGATCCCCATACCCCTGAGATGCCACCTCCAATGAGGGCATTCTTAGACCTTGAGGAATTGCAAAAAGATGCTCTGGGAATCTCCTCTTCCCATTTCCTCGGTGTCAAAAGGAATGGAGCTTATACTGATGAATCTGACTCTGCAACAAGCAGAAAGGGCTTGAAACAGCTGAATGATCTATTTGGGTCTGGAGAAGGAAGGGCGAAAAAAGGCCTCAATTTTCATGATGCAGAAGAAAAGGACCGCAGCATGCAGAACAATGGGACCCATGACATCAAGGCTCGATCATTGTCCGAGTCTGATCGACTGGGTAAAGCGGAGACAGAAATTTCAAACTTGAAGGTGGCCCTTGCTAAATTAGAAGCCGAAAAGGAAGCTGGCCTACTTCAGTACCAACAGTGTTTAGAGAGGTTAAATAATCTGGAGTCTGAAGCCTCTCGTGCACATGGGGATTCCAGGGGACTGAACGAACGAGCCAGGAAAGCTGAAGCTGAAGTTCAAGCTTTGAAGGAAGCACTTGTCAAATTGGAGTCTGAAAGGGATGCTAGTCTTCTGCAGTATCAGCAGTGTTTGGAGAAAATCACTGATCTGGAGAACAGTATCTCTCGTGCCCAAAAGGATGCTGGAGAGCTCAATGATCGCGCGAGTAAAGCTGAAACTGAAGCTGGAGCTCTAAAGCAAGATCTTGCAAAGGTCGTAGCAGAGAAAGAAGCTGCACTTGCACAATATCAACAATGTCTAGAAATGATACCAAATCTGGAGGAAAAAATACTGCATATTGAGGAGGATACTAGAAGAATTTGTGAACGAGCTGTCAAAGCCGAAGGTGAAGTTGAAACCTTGAAGCAAGCAATTGCCAAGTTAAATGAAGAGAAGGAAGCTGCTGCTCTCCAGTACCAGCAGTGCTTGGAAACCATATCTACACTAGAGCATAAGATAGCTAGTGCCCAAGAGGAGGCCCAACGGCTACACTCTGAAATAGCTGATGGGAATGCAAAGTTAAAGGGTTCCGAAGAAACGTGTATTCTGCTGGCACAATCTAATCAGACGCTCCAGTCTGAGTTGGAGTCTTTGGTGCAGAAAATGGAATCTCAGGGTGAAGAACTTACAGAGAAGCAGAAGGAGTTGGGCAGACTCTGGACTTGCATACAAGAAGAGCGTTTGCGATTCATGGAGGCAGAAACTGCTTTCCAGACACTGCAGCATTTGCATTCTCAATCCCAGGAGGAACTTAGATCTATGTATTCTGAGCTTCAGAATGGAGCTTTAATTATGAAGGACATGGAAACACGCAATCTAGTTTTGGAGGACGAAGTCCAGAAGGCGAAGGAGGAAAACAAGAGCCTGAGTGGGCTCAATCTGTCTTCATCCATGTCAATAAAAAATCTGCAAGATGAAATCTTAATCTTGAGGGAGACAATTAGGAAACTCGAAGAGGAAGTTGGACTTAGAGTAGACCAAAGAAACGCTCTCCAGCAAGAGATTTATTGCCTGAAAGAGGAACTCAATGATCTGAACAAGAAACACCAAGCTATGCTAGAGCAGGTCGAGTCAGTCGGCTTGGGTCCAGAGTGCCTCGTGTCATCTGTGAAAGAACTGCAGGATGAAAAATCACAGCTAGAACAGATGTGTGAGGCTGAGAGAAGTGTGAAAGCGGCACTGCTAGAAAAGTTGGAAATCATGCAGAAACTTGTGGAGAAAAATGTTCTTTTGGAGAATTCCCTTTCTGATTTGAACGTTGAGTTAGAAGGGGTTAGAGGGAAGGTAAGGGAGTTAGAAGAATCATGTCAATCTCATCTGGAAGAAAAAGGCACAATTGCTGCTGAAAATGCCGCCCTACTTTCTCAGTTACAGATTATGACTGAGAATTTGAAGAAATCTTCTGAGAATAACAACCTTTTGGAGAATTCCCTTTGCGATGCAAATGCTGAACTTGAAGGCTTGAGGGTAAAATCAAAGAGCTTAGAAGAGTGCTGTTTATTGCTTATTAACGAGAAGTCTGGTCTGATCATGGAGAGAGAAAATGTAGTTTCTGAGTTGGACGCTACTCGGCAAAGACTGGAAGGTCTGGGAAAAGGATATGCAGAAATAGAAGAGAAACTCTCTTCTCTGGAGAAGGAAAGAGAATTTGCACGTCGTAAAGTGGAAGAGCTACATGTTTTCTTAGATTCTGAGAAGCAAAAGCATGCTAGTTTTGTTCAGGTAAGTGAAACCCAGATGGCCGTTATGGGGTTGCAGATCTCTCATCTCCAAGCAGAAGGCATGTGCAGGAAGAAAGAATATGAAGTGGAACAAGACAAAGCTGTGAATGCGCAGATTGAAATCTTTATCTTGCAGAAATGCATAGAAGATCTGGAAGAAAATATCTTGTCCCTCATGGTTGAGCGTCAAAAGCTGTTGGAGGCATCCAAAATGTCAGAGAAGCGGATTTCTGACCTAGAGCATGGAAATCTTGAGCAGCAGATGGAGATTAAATCCTTTCTTTTGCAAACGAAAGTACTGAGAATGGGGCTGTATCAGGTGTTGAAGACTGTTGACGTGGATGCAAACCTCGATTGTGCAGGAGAGGTTGAGAAAGATGAAACACTTTTTAACCACATTCTTGTCAAACTTCAAGAGACGCAAAATTCTCTGTCTGAAACTTGTGATCAAAATCAACAGTTGGTTATTGAGAAGTCAGTTCTCATAGAAATGATTGACCAACTAAAACTAGAGGCGGCAAATCTTATGAGAGAAAGAAACACCCTGGACCGAGAGTTCAAGAACCAGTCTGAGAAGCTCGTGGTGTTGCAGAGTGGGGCCCAAAGACTTGAGGAGAAGAATGAAGAATTGAAGTTGAAAGTAGTGGAAGGCGATCGCAGAGAGGAAGTATTGAGGACTGAAATAGATGATCTGCACGAGCAGTTCTTGGACTTGCAAAGTGCACACAACAATCTATTGGAAGAGAATGGCAAGATGCTTGTAGAGAAAGGAGCTTTGGCAAGGATGGTATCGAATTTGTGGGAAGAGAATCGTGGCCTCGAAGAGGAGAAGTCTGTCATGTTTGGTGAAACGATATATCACAATAACTTCTCACTTGTTCTCAAAGATTTTATTTCCAGAAAGCTGCTGGAACTGGAAGAGCTCACTGACTATTTAGATAAACTTCATCTTGGAAAGAATGACCTGGAAGATAAGGTAAGAATATTGGAGGGGAAGTTGGAAGTTACATGGATGGACAACATACAGCTCAAGGAGTCATTAAATAAGTCCGAGAATGAGCTGGAGCTAGTTAAATATGTCAATGATCAATTGAATGGTGAAATTGAAAATGCAAAGGATGCAGTGTCTCATAAAGAAAATGAGCTTTTGGAAGTACATCAGGCCGTCAATGCGCTACAGAATGAGAAGCAAGAATTGCATGCGTTGGTGGAGGATCTGAGTGGTAATTATGATGAGGCTAAGGTGGTACTAGAACATCAAGAGAAGCAGATTTTTAAACTCTCTGCAGATAATGAACATCAAACCAAAGATACTTGCAGCCTTCGCGAAGTGAATCAAGAGTTGGAGTCTGAACTGCTGAAAATGCATGGAGAAGCTGAAAAAACCAAAACTAAAGAGGAAAGTTTGATCAATGAACTGCAAAAGGAAAGGCAAGAGATAGAAATGTGGTTGTTTCAGGCTGTCACATTCTTTGGCGAACTACAGACCTCCACCATTCGTGAAACATTGTTTGAAGGAAAAATCCGCGAGCTCATTGAAGCATGTCAGATACTCGAAGACAGAAGCAATTCCAATGGCATCGAGAACAAAATCATGAAAGAAAGAGTCAGGGCATTGGAAGACGAAAATGGAGGGCTACAAGCTCAGTTGGCTGCATATATTCCAGCTGTCATGTCTTTGAAGGAGTGCATAACATCACTTGAGAAGCATATGCTTGCAGACACCGGATCTCATAAACTTGACACTGCAGAATCAGAGGATGCCCTTCTGCACGCTGAAAGATCTCAAACGGATGGAGATCAAATTGCCACAGTATCAGATGGTGTTTTGGACTTGCAGAATTTACAGAGAAGGATTGAAGCTATTGAAAGGGCAGTGGTGGAGAAAGAAAATCATGTTTCCACAAATCGGGTTCGGAAGAAGTGTGAAATATCTGGATCAGGGAATGAAGTTCTGACAAAAGACATTGTTCTTGATCACAGATCGGAGTGTTCATCCTATGAGGTAAGCAGGAGAGAAACCACAGAGCCTGATGCTCAGATGCTTGAGTTGTGGGAGACCTCTGACCAGGATGACAGCATTGATTTGATGGTTGGCAAATCCCAGAAGGGGGCCGCGGTACCTACTGACCACAGTCAGATGGAGGCAGTGAAGGAGCACAAGAAAAAACACCCCTCTTCTGAATCATTGGTTGAGAAAGAATTGGGCATTGACAAATTAGAGCTTTCTAGGAGATTTACTCAGCCCCGTCAAGAAGGGAACAAGAGAAGGATCCTAGAAAGACTCGACTCCGATGTGCAAAAGTTGACAAACCTTCAGATAACCGTGGAAGATCTTAAGACAAAGGTGGAGATTACTGAACAGAGCAAAAATGGCAAAGATATGGAACTTGATAGCGTAAAGGGGCAGCTGGAAGAAGCTGAAGAGGCCATTACAAAGTTGTTTGACGCCAATCAAAAGTTGATGAAGAGCGTTGAAGATGCTCCTCCGTCCTCGGATGGAGCTTCTGGGGAAGTTCCAGATGAAAGTGGGAGCGTGAGAAGGAGGAGACTTTCAGAACAAGCAAAAAGAGGGTCGGAAAAAATTGGGCGGTTGCAGTTGCAGGTGCAGAAACTGCAGTTTCTTCTTCTGAAAATCGATGGCAAAACTGACAGCAAAGGATCAGCTAGAATAATCGAGCGGAAGAAAAGCGTTCTTCTACGTGACTACCTCTATGGCGTGAGAAAACCCGTCAACCAAGGGAAACGGAAGAAAGCGCCCTTCTGCGCATGCATGCAGCCCCCAACCAAGGGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIP1

Protein Analysis

1792

Amino Acids

203.8

Weight (kDa)

5.02

Isoelectric Point (pI)

52.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 14 - 87 2.6e-36 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 5170
AasI GACNNNNNNGTC 3 cut(s) 10, 1429, 2439
Acc16I TGCGCA 2 cut(s) 2688, 5344
Acc36I ACCTGC 3 cut(s) 344, 1909, 5170
Acc65I GGTACC 1 cut(s) 4696
AccB1I GGYRCC 1 cut(s) 4696
AccBSI CCGCTC 1 cut(s) 5257
AccI GTMKAC 1 cut(s) 1830
AccII CGCG 6 cut(s) 11, 1017, 1019, 3903, 4104, 4694
AclI AACGTT 1 cut(s) 2112
AclWI GGATC 7 cut(s) 371, 3798, 4312, 4522, 4843, 4856, 5245
AcoI YGGCCR 1 cut(s) 2595
AcuI CTGAAG 9 cut(s) 71, 746, 825, 882, 888, 1056, 1613, 4877, 5019
AcyI GRCGYC 1 cut(s) 5025
AfaI GTAC 7 cut(s) 182, 767, 1271, 2874, 3732, 3825, 4698
AflII CTTAAG 1 cut(s) 4913
AflIII ACRYGT 2 cut(s) 1394, 2530
AjiI CACGTC 2 cut(s) 2510, 2914
AjnI CCWGG 7 cut(s) 282, 830, 851, 1596, 3120, 3540, 4644
AjuI GAANNNNNNNTTGG 6 cut(s) 1800, 1832, 3335, 3367, 3902, 3934
AloI GAACNNNNNNTCC 2 cut(s) 3116, 3148
Alw21I GWGCWC 7 cut(s) 820, 1008, 1048, 3301, 3501, 4110, 4737
Alw26I GTCTC 6 cut(s) 689, 1784, 2987, 3708, 4628, 5119
Alw44I GTGCAC 2 cut(s) 816, 3297
AlwI GGATC 7 cut(s) 371, 3798, 4312, 4522, 4843, 4856, 5245
AlwNI CAGNNNCTG 5 cut(s) 1512, 1559, 1573, 4607, 5191
ApaI GGGCCC 1 cut(s) 3178
ApaLI GTGCAC 2 cut(s) 816, 3297
ArsI GACNNNNNNTTYG 4 cut(s) 2227, 2259, 5135, 5167
AseI ATTAAT 1 cut(s) 203
AsiSI GCGATCGC 1 cut(s) 3232
Asp700I GAANNNNTTC 2 cut(s) 309, 4144
Asp718I GGTACC 1 cut(s) 4696
AspLEI GCGC 8 cut(s) 195, 277, 282, 1019, 2689, 3751, 5335, 5345
AsuHPI GGTGA 5 cut(s) 339, 1214, 1484, 3434, 3686
AsuII TTCGAA 1 cut(s) 77
AvaII GGWCC 4 cut(s) 616, 639, 1940, 3124
BaeGI GKGCMC 5 cut(s) 820, 988, 1324, 3178, 3301
BaeI ACNNNNGTAYC 2 cut(s) 749, 782
BamHI GGATCC 1 cut(s) 4848
BanI GGYRCC 1 cut(s) 4696
BanII GRGCYC 6 cut(s) 1008, 1048, 1731, 3178, 3501, 4110
BarI GAAGNNNNNNTAC 2 cut(s) 741, 773
BauI CACGAG 5 cut(s) 813, 981, 1952, 3155, 3272
BbsI GAAGAC 5 cut(s) 917, 1731, 2909, 4140, 4203
Bbv12I GWGCWC 7 cut(s) 820, 1008, 1048, 3301, 3501, 4110, 4737
BceAI ACGGC 3 cut(s) 1355, 2582, 3725
BcgI CGANNNNNNTGC 2 cut(s) 2282, 2316
BciT130I CCWGG 7 cut(s) 284, 832, 853, 1598, 3122, 3542, 4646
BciVI GTATCC 1 cut(s) 1156
BclI TGATCA 7 cut(s) 129, 259, 2382, 3022, 3661, 3984, 4552
BcoDI GTCTC 6 cut(s) 689, 1784, 2987, 3708, 4628, 5119
BfmI CTRYAG 8 cut(s) 929, 1574, 1973, 3752, 3861, 4058, 4323, 5192
BfoI RGCGCY 3 cut(s) 196, 278, 5336
BfrI CTTAAG 1 cut(s) 4913
BfuAI ACCTGC 3 cut(s) 344, 1909, 5170
BfuI GTATCC 1 cut(s) 1156
BglII AGATCT 6 cut(s) 1057, 1610, 2613, 2725, 4358, 4909
BmcAI AGTACT 1 cut(s) 2874
Bme1390I CCNGG 7 cut(s) 284, 832, 853, 1598, 3122, 3542, 4646
Bme18I GGWCC 4 cut(s) 616, 639, 1940, 3124
BmgBI CACGTC 2 cut(s) 2510, 2914
BmrFI CCNGG 7 cut(s) 284, 832, 853, 1598, 3122, 3542, 4646
BmrI ACTGGG 2 cut(s) 279, 692
BmuI ACTGGG 2 cut(s) 279, 692
BoxI GACNNNNGTC 1 cut(s) 4710
BpiI GAAGAC 5 cut(s) 917, 1731, 2909, 4140, 4203
BplI GAGNNNNNCTC 4 cut(s) 2462, 2494, 4801, 4833
Bpu10I CCTNAGC 1 cut(s) 3816
Bpu14I TTCGAA 1 cut(s) 77
BpuEI CTTGAG 8 cut(s) 446, 1804, 2338, 2849, 3206, 3590, 4298, 4643
Bsa29I ATCGAT 1 cut(s) 5214
BsaAI YACGTR 1 cut(s) 5279
BsaBI GATNNNNATC 3 cut(s) 2696, 3027, 4374
BsaHI GRCGYC 1 cut(s) 5025
BsaI GGTCTC 1 cut(s) 4628
BsaWI WCCGGW 1 cut(s) 4301
BsaXI ACNNNNNCTCC 2 cut(s) 3557, 3587
Bse118I RCCGGY 1 cut(s) 349
Bse1I ACTGG 7 cut(s) 274, 687, 1267, 1429, 2439, 3142, 3495
Bse3DI GCAATG 1 cut(s) 339
Bse8I GATNNNNATC 3 cut(s) 2696, 3027, 4374
BseBI CCWGG 7 cut(s) 284, 832, 853, 1598, 3122, 3542, 4646
BseCI ATCGAT 1 cut(s) 5214
BseJI GATNNNNATC 3 cut(s) 2696, 3027, 4374
BseMI GCAATG 1 cut(s) 339
BseNI ACTGG 7 cut(s) 274, 687, 1267, 1429, 2439, 3142, 3495
BseRI GAGGAG 7 cut(s) 448, 1172, 1343, 3203, 3416, 5052, 5137
BseSI GKGCMC 5 cut(s) 820, 988, 1324, 3178, 3301
BsgI GTGCAG 7 cut(s) 1074, 1472, 2662, 2955, 3254, 4331, 5204
Bsh1236I CGCG 6 cut(s) 11, 1017, 1019, 3903, 4104, 4694
Bsh1285I CGRYCG 1 cut(s) 3232
BshNI GGYRCC 1 cut(s) 4696
BshVI ATCGAT 1 cut(s) 5214
BsiEI CGRYCG 1 cut(s) 3232
BsiHKAI GWGCWC 7 cut(s) 820, 1008, 1048, 3301, 3501, 4110, 4737
BsiSI CCGG 2 cut(s) 350, 4302
BslFI GGGAC 3 cut(s) 652, 849, 2731
BsmAI GTCTC 6 cut(s) 689, 1784, 2987, 3708, 4628, 5119
BsmBI CGTCTC 1 cut(s) 2987
BsmFI GGGAC 3 cut(s) 652, 849, 2731
BsmI GAATGC 4 cut(s) 413, 1372, 1585, 2689
Bso31I GGTCTC 1 cut(s) 4628
Bsp119I TTCGAA 1 cut(s) 77
Bsp120I GGGCCC 1 cut(s) 3174
Bsp19I CCATGG 1 cut(s) 297
Bsp68I TCGCGA 1 cut(s) 3903
BspDI ATCGAT 1 cut(s) 5214
BspFNI CGCG 6 cut(s) 11, 1017, 1019, 3903, 4104, 4694
BspHI TCATGA 2 cut(s) 598, 4170
BspMAI CTGCAG 6 cut(s) 933, 1578, 1977, 3865, 4327, 5196
BspMI ACCTGC 3 cut(s) 344, 1909, 5170
BspPI GGATC 7 cut(s) 371, 3798, 4312, 4522, 4843, 4856, 5245
BspQI GCTCTTC 4 cut(s) 1524, 2517, 3489, 5039
BspT104I TTCGAA 1 cut(s) 77
BspT107I GGYRCC 1 cut(s) 4696
BspTI CTTAAG 1 cut(s) 4913
BspTNI GGTCTC 1 cut(s) 4628
BsrBI CCGCTC 1 cut(s) 5257
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 349
BsrI ACTGG 7 cut(s) 274, 687, 1267, 1429, 2439, 3142, 3495
BssAI RCCGGY 1 cut(s) 349
BssNI GRCGYC 1 cut(s) 5025
BssSI CACGAG 5 cut(s) 813, 981, 1952, 3155, 3272
BssT1I CCWWGG 3 cut(s) 297, 5314, 5364
Bst2BI CACGAG 5 cut(s) 813, 981, 1952, 3155, 3272
Bst2UI CCWGG 7 cut(s) 284, 832, 853, 1598, 3122, 3542, 4646
Bst4CI ACNGT 8 cut(s) 239, 774, 974, 2908, 3036, 4390, 4712, 4903
BstACI GRCGYC 1 cut(s) 5025
BstAFI CTTAAG 1 cut(s) 4913
BstAPI GCANNNNNTGC 6 cut(s) 239, 1559, 1582, 3695, 3772, 5191
BstBAI YACGTR 1 cut(s) 5279
BstBI TTCGAA 1 cut(s) 77
BstDSI CCRYGG 3 cut(s) 297, 4692, 4902
BstENI CCTNNNNNAGG 3 cut(s) 163, 1871, 3398
BstFNI CGCG 6 cut(s) 11, 1017, 1019, 3903, 4104, 4694
BstH2I RGCGCY 3 cut(s) 196, 278, 5336
BstHHI GCGC 8 cut(s) 195, 277, 282, 1019, 2689, 3751, 5335, 5345
BstMAI GTCTC 6 cut(s) 689, 1784, 2987, 3708, 4628, 5119
BstMCI CGRYCG 1 cut(s) 3232
BstNI CCWGG 7 cut(s) 284, 832, 853, 1598, 3122, 3542, 4646
BstNSI RCATGY 8 cut(s) 628, 2534, 2564, 2641, 3779, 4122, 5349, 5353
BstPAI GACNNNNGTC 1 cut(s) 4710
BstSCI CCNGG 7 cut(s) 282, 830, 851, 1596, 3120, 3540, 4644
BstSFI CTRYAG 8 cut(s) 929, 1574, 1973, 3752, 3861, 4058, 4323, 5192
BstSLI GKGCMC 5 cut(s) 820, 988, 1324, 3178, 3301
BstUI CGCG 6 cut(s) 11, 1017, 1019, 3903, 4104, 4694
BstV2I GAAGAC 5 cut(s) 917, 1731, 2909, 4140, 4203
BstX2I RGATCY 9 cut(s) 1057, 1610, 2613, 2725, 3790, 4304, 4358, 4848, 4909
BstYI RGATCY 9 cut(s) 1057, 1610, 2613, 2725, 3790, 4304, 4358, 4848, 4909
Bsu15I ATCGAT 1 cut(s) 5214
BsuI GTATCC 1 cut(s) 1156
BsuTUI ATCGAT 1 cut(s) 5214
BtgI CCRYGG 3 cut(s) 297, 4692, 4902
BtgZI GCGATG 1 cut(s) 2307
BtrI CACGTC 2 cut(s) 2510, 2914
BtsI GCAGTG 8 cut(s) 947, 1283, 1571, 2033, 3705, 4320, 4458, 4731
BtuMI TCGCGA 1 cut(s) 3903
BveI ACCTGC 3 cut(s) 344, 1909, 5170
CaiI CAGNNNCTG 5 cut(s) 1512, 1559, 1573, 4607, 5191
CciI TCATGA 2 cut(s) 598, 4170
CfoI GCGC 8 cut(s) 195, 277, 282, 1019, 2689, 3751, 5335, 5345
Cfr10I RCCGGY 1 cut(s) 349
Cfr42I CCGCGG 1 cut(s) 4695
ClaI ATCGAT 1 cut(s) 5214
CseI GACGC 6 cut(s) 39, 1432, 2426, 2750, 3004, 5033
Csp6I GTAC 7 cut(s) 181, 766, 1270, 2873, 3731, 3824, 4697
CviQI GTAC 7 cut(s) 181, 766, 1270, 2873, 3731, 3824, 4697
DraI TTTAAA 1 cut(s) 3853
DrdI GACNNNNNNGTC 3 cut(s) 10, 1429, 2439
DseDI GACNNNNNNGTC 3 cut(s) 10, 1429, 2439
EaeI YGGCCR 1 cut(s) 2595
Ecl136II GAGCTC 4 cut(s) 1006, 1046, 3499, 4108
Eco130I CCWWGG 3 cut(s) 297, 5314, 5364
Eco147I AGGCCT 1 cut(s) 588
Eco24I GRGCYC 6 cut(s) 1008, 1048, 1731, 3178, 3501, 4110
Eco31I GGTCTC 1 cut(s) 4628
Eco47I GGWCC 4 cut(s) 616, 639, 1940, 3124
Eco53kI GAGCTC 4 cut(s) 1006, 1046, 3499, 4108
Eco57I CTGAAG 9 cut(s) 71, 746, 825, 882, 888, 1056, 1613, 4877, 5019
EcoICRI GAGCTC 4 cut(s) 1006, 1046, 3499, 4108
EcoNI CCTNNNNNAGG 3 cut(s) 163, 1871, 3398
EcoO109I RGGNCCY 2 cut(s) 639, 3174
EcoRI GAATTC 3 cut(s) 73, 2092, 2281
EcoRII CCWGG 7 cut(s) 282, 830, 851, 1596, 3120, 3540, 4644
EcoT14I CCWWGG 3 cut(s) 297, 5314, 5364
EcoT22I ATGCAT 3 cut(s) 2720, 3945, 5351
EcoT38I GRGCYC 6 cut(s) 1008, 1048, 1731, 3178, 3501, 4110
ErhI CCWWGG 3 cut(s) 297, 5314, 5364
Esp3I CGTCTC 1 cut(s) 2987
FalI AAGNNNNNCTT 4 cut(s) 1077, 1109, 1806, 1838
FaqI GGGAC 3 cut(s) 652, 849, 2731
FauNDI CATATG 1 cut(s) 4287
FbaI TGATCA 7 cut(s) 129, 259, 2382, 3022, 3661, 3984, 4552
FblI GTMKAC 1 cut(s) 1830
FriOI GRGCYC 6 cut(s) 1008, 1048, 1731, 3178, 3501, 4110
FspI TGCGCA 2 cut(s) 2688, 5344
GlaI GCGC 8 cut(s) 194, 276, 281, 1018, 2688, 3750, 5334, 5344
HaeII RGCGCY 3 cut(s) 196, 278, 5336
HapII CCGG 2 cut(s) 350, 4302
HgaI GACGC 6 cut(s) 39, 1432, 2426, 2750, 3004, 5033
HhaI GCGC 8 cut(s) 195, 277, 282, 1019, 2689, 3751, 5335, 5345
Hin1I GRCGYC 1 cut(s) 5025
Hin6I GCGC 8 cut(s) 193, 275, 280, 1017, 2687, 3749, 5333, 5343
HinP1I GCGC 8 cut(s) 193, 275, 280, 1017, 2687, 3749, 5333, 5343
HincII GTYRAC 3 cut(s) 2911, 4884, 5311
HindII GTYRAC 3 cut(s) 2911, 4884, 5311
HindIII AAGCTT 1 cut(s) 876
HpaII CCGG 2 cut(s) 350, 4302
HphI GGTGA 5 cut(s) 339, 1214, 1484, 3434, 3686
Hpy166II GTNNAC 7 cut(s) 818, 1181, 1831, 2911, 3299, 4884, 5311
Hpy8I GTNNAC 7 cut(s) 818, 1181, 1831, 2911, 3299, 4884, 5311
Hpy99I CGWCG 2 cut(s) 16, 2514
HpyCH4III ACNGT 8 cut(s) 239, 774, 974, 2908, 3036, 4390, 4712, 4903
HpyCH4IV ACGT 5 cut(s) 1394, 2112, 2509, 2913, 5278
HpySE526I ACGT 5 cut(s) 1394, 2112, 2509, 2913, 5278
Hsp92I GRCGYC 1 cut(s) 5025
HspAI GCGC 8 cut(s) 193, 275, 280, 1017, 2687, 3749, 5333, 5343
KflI GGGWCCC 1 cut(s) 639
KpnI GGTACC 1 cut(s) 4700
Ksp22I TGATCA 7 cut(s) 129, 259, 2382, 3022, 3661, 3984, 4552
KspI CCGCGG 1 cut(s) 4695
LguI GCTCTTC 4 cut(s) 1524, 2517, 3489, 5039
MaeII ACGT 5 cut(s) 1394, 2112, 2509, 2913, 5278
MaeIII GTNAC 4 cut(s) 2223, 3580, 4039, 5279
MbiI CCGCTC 1 cut(s) 5257
MfeI CAATTG 3 cut(s) 1227, 2190, 3665
MflI RGATCY 9 cut(s) 1057, 1610, 2613, 2725, 3790, 4304, 4358, 4848, 4909
MmeI TCCRAC 8 cut(s) 1419, 1798, 2028, 2394, 2754, 3555, 3900, 5133
Mph1103I ATGCAT 3 cut(s) 2720, 3945, 5351
MroXI GAANNNNTTC 2 cut(s) 309, 4144
MslI CAYNNNNRTG 1 cut(s) 3065
MspA1I CMGCKG 4 cut(s) 544, 4244, 4694, 4987
MspCI CTTAAG 1 cut(s) 4913
MspI CCGG 2 cut(s) 350, 4302
MspR9I CCNGG 7 cut(s) 284, 832, 853, 1598, 3122, 3542, 4646
MteI GCGCNGCGC 1 cut(s) 278
MunI CAATTG 3 cut(s) 1227, 2190, 3665
Mva1269I GAATGC 4 cut(s) 413, 1372, 1585, 2689
MvaI CCWGG 7 cut(s) 284, 832, 853, 1598, 3122, 3542, 4646
MvnI CGCG 6 cut(s) 11, 1017, 1019, 3903, 4104, 4694
NcoI CCATGG 1 cut(s) 297
NdeI CATATG 1 cut(s) 4287
NmeAIII GCCGAG 2 cut(s) 199, 2404
NmuCI GTSAC 2 cut(s) 4039, 5279
NruI TCGCGA 1 cut(s) 3903
NsbI TGCGCA 2 cut(s) 2688, 5344
NsiI ATGCAT 3 cut(s) 2720, 3945, 5351
NspI RCATGY 8 cut(s) 628, 2534, 2564, 2641, 3779, 4122, 5349, 5353
NspV TTCGAA 1 cut(s) 77
PaeI GCATGC 5 cut(s) 628, 2564, 3779, 5349, 5353
PagI TCATGA 2 cut(s) 598, 4170
PaqCI CACCTGC 1 cut(s) 5170
PceI AGGCCT 1 cut(s) 588
PciI ACATGT 1 cut(s) 2530
PciSI GCTCTTC 4 cut(s) 1524, 2517, 3489, 5039
PcsI WCGNNNNNNNCGW 1 cut(s) 235
PctI GAATGC 4 cut(s) 413, 1372, 1585, 2689
PdmI GAANNNNTTC 2 cut(s) 309, 4144
PflFI GACNNNGTC 1 cut(s) 1688
Ple19I CGATCG 1 cut(s) 3232
Ppu21I YACGTR 1 cut(s) 5279
PpuMI RGGWCCY 1 cut(s) 639
PscI ACATGT 1 cut(s) 2530
PshAI GACNNNNGTC 1 cut(s) 4710
PshBI ATTAAT 1 cut(s) 203
Psp124BI GAGCTC 4 cut(s) 1008, 1048, 3501, 4110
Psp1406I AACGTT 1 cut(s) 2112
Psp5II RGGWCCY 1 cut(s) 639
Psp6I CCWGG 7 cut(s) 282, 830, 851, 1596, 3120, 3540, 4644
PspGI CCWGG 7 cut(s) 282, 830, 851, 1596, 3120, 3540, 4644
PspOMI GGGCCC 1 cut(s) 3174
PspPPI RGGWCCY 1 cut(s) 639
PstI CTGCAG 6 cut(s) 933, 1578, 1977, 3865, 4327, 5196
PstNI CAGNNNCTG 5 cut(s) 1512, 1559, 1573, 4607, 5191
PsuI RGATCY 9 cut(s) 1057, 1610, 2613, 2725, 3790, 4304, 4358, 4848, 4909
PsyI GACNNNGTC 1 cut(s) 1688
PvuI CGATCG 1 cut(s) 3232
PvuII CAGCTG 3 cut(s) 544, 4244, 4987
RgaI GCGATCGC 1 cut(s) 3232
RruI TCGCGA 1 cut(s) 3903
RsaI GTAC 7 cut(s) 182, 767, 1271, 2874, 3732, 3825, 4698
RsaNI GTAC 7 cut(s) 181, 766, 1270, 2873, 3731, 3824, 4697
RseI CAYNNNNRTG 1 cut(s) 3065
SacI GAGCTC 4 cut(s) 1008, 1048, 3501, 4110
SacII CCGCGG 1 cut(s) 4695
SapI GCTCTTC 4 cut(s) 1524, 2517, 3489, 5039
ScaI AGTACT 1 cut(s) 2874
ScrFI CCNGG 7 cut(s) 284, 832, 853, 1598, 3122, 3542, 4646
SfaAI GCGATCGC 1 cut(s) 3232
SfcI CTRYAG 8 cut(s) 929, 1574, 1973, 3752, 3861, 4058, 4323, 5192
Sfr303I CCGCGG 1 cut(s) 4695
SfuI TTCGAA 1 cut(s) 77
SgfI GCGATCGC 1 cut(s) 3232
SgrBI CCGCGG 1 cut(s) 4695
SinI GGWCC 4 cut(s) 616, 639, 1940, 3124
SmiMI CAYNNNNRTG 1 cut(s) 3065
SmlI CTYRAG 9 cut(s) 425, 1783, 2317, 2828, 3185, 3605, 4277, 4622, 4913
SmoI CTYRAG 9 cut(s) 425, 1783, 2317, 2828, 3185, 3605, 4277, 4622, 4913
SphI GCATGC 5 cut(s) 628, 2564, 3779, 5349, 5353
SseBI AGGCCT 1 cut(s) 588
SspI AATATT 1 cut(s) 3561
SstI GAGCTC 4 cut(s) 1008, 1048, 3501, 4110
StuI AGGCCT 1 cut(s) 588
StyD4I CCNGG 7 cut(s) 282, 830, 851, 1596, 3120, 3540, 4644
StyI CCWWGG 3 cut(s) 297, 5314, 5364
TaaI ACNGT 8 cut(s) 239, 774, 974, 2908, 3036, 4390, 4712, 4903
TaiI ACGT 5 cut(s) 1397, 2115, 2512, 2916, 5281
TaqII GACCGA 1 cut(s) 3141
TatI WGTACW 3 cut(s) 180, 2872, 3730
TauI GCSGC 4 cut(s) 2033, 2210, 3095, 4694
TseFI GTSAC 2 cut(s) 4039, 5279
Tsp45I GTSAC 2 cut(s) 4039, 5279
TspGWI ACGGA 3 cut(s) 4382, 5055, 5338
Tth111I GACNNNGTC 1 cut(s) 1688
Vha464I CTTAAG 1 cut(s) 4913
VneI GTGCAC 2 cut(s) 816, 3297
VpaK11BI GGWCC 4 cut(s) 616, 639, 1940, 3124
VspI ATTAAT 1 cut(s) 203
XagI CCTNNNNNAGG 3 cut(s) 163, 1871, 3398
XbaI TCTAGA 2 cut(s) 26, 1114
XceI RCATGY 8 cut(s) 628, 2534, 2564, 2641, 3779, 4122, 5349, 5353
XcmI CCANNNNNNNNNTGG 2 cut(s) 995, 3481
XmiI GTMKAC 1 cut(s) 1830
XmnI GAANNNNTTC 2 cut(s) 309, 4144
ZrmI AGTACT 1 cut(s) 2874
Zsp2I ATGCAT 3 cut(s) 2720, 3945, 5351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.