MD03G1260100.v1.1

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005688
Physical Location & Seq
Reverse (-)
34697890 .. 34705131
7242 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1260100.v1.1.491

Sequence Viewer

Length: 5385 bp
ATGGCTACCGCGTCGCAGGCAGACTCTAGACGCAAGTATTCTTGGTGGTGGGACAGCCACATAAGCCCGAAGAATTCAAGATGGCTTCAGGAAAATCTTACAGATATGGATGCCAAAGTCAAACACATGATCAAGCTCATTGAATTGGATGCCGATTCCTTTGCTAGGAGGGCAGAGATGTACTATAAACAGCGCCCAGAATTAATGAAATTGGTAGAAGAGTTCTATCGAGCATACCGTGCATTAGCTGAGAGATATGATCATGCAACTGGGGCGCTGCGCCAGGCTCATCGAACCATGGCAGAAGCATTTCCCAACCAAGTTCCCTTTGCAATGGGAGATGATTCACCTGCAGGTTCCTCTGCTAGTGAGGCTGATCCCCGTACACCTGAGATGCCACATCCAATGCGGGCATTCTTAGACCTTGATGAAATGCCAAAAGATGCTCCGGGAATCTCCTCATCCCATTTCCTTGGTGTAAAAAAGAATGGAGCTTATACCGATGAATCTGACTCTGGAACAAGCAGAATGGGCTTGAAACAGCTGAATGATCTATTTGGGTCTGGAGAAGGAAGGGCGAAAAAAGGTCTCAATTTTCCTGATGAAGAAGAAAAAGACCGCAGCATGCAGGACAATGAGACCCATAACGTCAAGGCTCGATCATTGTCCGAGTCTGATCGACTGGGTAAAGCAGAGACAGAAATTTCAAACTTGAAGGTGGCCCTTGCTAAATTAGAAGCCGAAAAGGAAGCTGGCCTACTTCAGTACCAACAGTGTTTAGAGAGGTTAAATAATCTGGAGTCTGAAGTCGCTTGTGCACATGAGGATTCCAGGGGACTGAACAAGCGAGCCAGTAAAGCTGAAGCTGAAGTTCAAGCTTCGAAGGAAGCACTTGCTAAATTAGAGTCTGAAAGGGATGCTAGCCTTCTGCAGTATCAGCAGTGTTTGGAGAAAATCACTAATCTGGAGGACAGTATCTCTTGTGCCCAAAAGGATGCTGCAGAGCTCAATGATCGCGCGAGTAAAGTTGAAACTGAAGCTGGAACTCTAAAGCAAGATCTTGCAAAGGTCGTAGATGAGAAAGAAGCTGCGCTTGCACAATATCAACAATGTCTAGAAATGATATCAAATCTGGAGGAAAAAATACTGCATATTGAGGAGGATACAAGAAGAATTAATGAGCGAGCTGTTAAAGCCGAAGGTGAAGTTGAAAACTTGAAGCAAGCAATTGCCAAGTTAAATGAAGAGAAGGAAGCTGCTGCTCTCCAGTACCAGCAGTGCTTGGAAACCATATCTACTCTGGAGTATAAGATAGCTAGTGCCCAAGAGGAGGCCCGACGGCTACACTCTGAAATAGATGATGGGATTGCAAAGTTAAAGGGTTCTGAAGAAAAGTGTATTCTGCTGGCACAATCAAATCAGACGCTCCAATCTGAGTTGGAGTCTTTGGTGCAGAAAATGGAATCTCAGGGTGAAGAACTGACTGAGAAGCAGAAGGAGTTGGGCAGACTCTGGACTTCCATACGAGAAGAGCGTTTGCGATTCATGGAGGCTGAAACTGCTTTCCAGACACTGCAGCATTTGCATTCTCAATCCCAGGAGGAACTTAGATCTATGTATTCTGAACTTCACAATGGAGCTTTAATTATGAAGGACATGGAAACGCGTAATCAGGTTTTGGAGGACGAAGTCCAGAATTCGAAGGAGGAAAACAAGAGGCTGAGTGAGTTCAATCTGTCTGCATCCATGTCGATAAAAAATCTGCAAGATGAGATCTTAATCTTGAGGGAGACGATCAGAAAACTTGAAGAGGAACTCGTACTCCGAGTAGACCAAAGAAACGCTCTCCAGCAAGAGATATACTGCCTGAAAGAGGAACTCAATGGCTTGAACAAGAAACACCAAGTTATGCTAGAGCAGGTAGAGTCAGTTGGCTTGAATCCAGAGTGCCTTGCGTCATCTGTGAAGGAACTGCAGGGTGAAAAATCACAGCTAGAACAGATGTGTGAGGCTGATAGAAGTGAGAAAGCGGCACTGTTAGAAAAGTTGGAAATCATGCAGAAACTTATGGAGAAAAATGTTCTTTTGGAGAATTCCCTTTCTGATTTGAACCTTGAGTTAGAAGGTGTTAGAGGGAAGGTAAAGGAGTTGGAAGAATCATGTCAATCTCATCTAGAAGAAAAAGGTACAATTGCTGCTGAAAATGCCGCCCTACTTTCCCAGTTAAAAATTATGACCGAGAACTTGAAGAAATCATCGGAGAATAACAAAATTTTGGAGAATTCCCTTTGTGATGCAAATGCTGAACTTGAAGGCTTGAGGGTAAAATCAAAGAGCTTAGAAGAGTCCTGTCTATTGCTTGATAACGAGAAGTCTGGTCTGACCAAGGAGAGAGAAAATGTAGTTTCTGAGTTGGGCGCTACTCAGCGAAGACTGGCAGGTCTGGAAAAAGGATATACAGAAGTAGAAGAGAAACTCTCTGCTCTTGAGAAAGAGAGAGAATCCGCACTTCGTAAAGTGGAAGAGCTACATGTTTTCTTAGATTCTGAGAAACAAAAACATGCCAGTTTTGTTCAGTTGAGTGAAACCCAGATGGCTGGTATGGGATTGCAGATCTCTCGTCTCCAAGCAGAAGGCATGTGCATGAAGAAAGTATATGAAGTGGAACAAGACAAAGCTGTGAACGCGCAGATTGAAATCTTTGTCTTGCAGAAATGCATAAAAGATCTGGAAGAAAATATCTTGTCCCTCATGGTTGAGCGTCAAAAGCTGTTGGAGGCATCCAAAATGTCAGAGAAACAGATTTCTGACCTAGAGCATGGAAATCTTGAGCAACAGGTGGAGATTAAATCTTTCCTTTTGCAAACGAAAGTACTGAGAATGGGACTGTATCAGGTGTTGAAGACTCTTGACGTGGAGGCAAACCTCAGTTGTGCAGGAGAGGTTGAGCAAGACGAAACACTTCTTAACAACATTCTTGTCAAACTTCAAGAGACGCAAAATTCTCTCTCTGAAACTTGTGATCAAAATCAGCAGTTGGTTATTGAGAAGTCGGTTCTCATAGAAATGATTGACCAACTAAAACTAGAGGCAGCAAATCTTATGAGAGAAAGAAACACCCTGGACGGAGAGTTCAAGAATCAGTCTGAGAAGCTCATGGTGTTACAGAGTGGGGCCCAAAGACTTGAGGAGAAGAATGAAGAATTGAAGTTGAAAGTAGTGGAGAGAGACCACAGAGAGCAAGTGTTGAGGACTGAAATAGATGATCTGCATGAGCAGTTCTTGGACTTGCAAAGTGCATACAACGATGTATTGGAAGAGAATGGCAAGATGCTTGTAGAGAAAGGAGCTTTGACAAAGATGGTATCGAATTTGTGGGAAGAGAACCGTGACCTCGAAGAGGAGAAATGTGTCATGTTTGGTGAAACGATATATCACAATAACTTTTCACTTGTTCTCAAAGATTTTATTTCAAGAAAGCTGCAGGAACTGGAAGAGCTCACTGACTATTTAGATAAACTTCATCTTGGAAAGAATGACCTGGAAGATAAGGTAAGGATGTTGGAGGGGAAGTTAGAAGATACACGGATGGACAACGTACAGCTCAAGGAGTCATTGAATAAGTCCGAGAATGAGCTGGAGTTAGTTAAATCTGTTAATGATCAATTGAATGGTGAAATCGCAAATGCAAAGGATGCGTCGTACCATAAAGAAAATGAGCTTTTGGAAGTACATCAGGCCATCAGTGTGCTACAGAATGAGAGGCAAGAGTTGCATGCGTTAGTGGAGGACATGAATGGTAAGTATGGTGAGGCCGAGGTGGTACTACAAGATCAAGAGAAGCAAATTCTCAAACTCTCTGCAGACAATGAACTTCATATCAAAGAGACTGGCAACCTTTGTGTAGTGAATCAGGAGTTGGAGTCAGAAGTGCAGAAAATGCAGCAAAAAGCTGAAAAAACTAAAATCAAAGAGGAAGGTTTGATCAATGAACTGCAAAAGGAAAGACAAGAGATAGAAATGTGGTTGTTTCAGGCTGCCACATTATTTGGCGAACTGCAGGGTTCCAACATTCGTGAAACATTGTTTGAAGGAAAGATTCGCGAGCTCATTGAAGCATGTCAGATCCTCGAAGACAGAAGCAATTCCAGAGGCATCGAGAACAAAATTTTGAAAGAAAGAGTTTGCGCATTGGAAGACGAAAATGGAGGGCTACAAGCTCAGTTGGCTGCATATATTCCAGCTGTCATCTCTTTGAAGGAGTGTATAACATCGCTTGAGAAGCATATACTCGCAGACACCGGTTCTCATAAACTTGACACTGAAGAATCAAAGGATGCCCTTCTGCATGCTGAAAGCCCTCAAATGGATGGAGATCAAACAGCCACAGTATCACATGGTGTTTTGGACTTGCAGGATTTACAAAGAAGACTTGAAGCTATTGAAAAGGCAGTGGTGGAAAAGGAAGGTCATGTTTCTGTAAATCAGGTTCGTGACACTATGAAGAAGCGTGAAATATCTGGATCAGGGAATGAAGTTCTGACAAAAGACATCGTTCTTGATCAGATATCGGAGTGTTCATCCTATGGGATAAGCAGAAGAGAAACCATAGAGCCTGATGCTCAGATGCTTGAGTTGTGGGAGACCAGTGACCAGGATGGCAGCATTGATTTGACAGTTGGCAACTCCCAGAAGGCAGCCGCTGTACCAACTGACCACAGTCAGATGGAGGCAGTAAAGGAGCACAATAAAAGACATCCCTCTTCTGAATCATTGGTTGAGAAAGAATTGGGTATTAACAAATTAGAACTCTCTAGGAGATTTACGCAGCCCCGTCAAGAAGGGAACAAGAGAAGCATCCTAGAAAGACTTGATTCCGATGTGCAAAAGTTGACAAACCTTCAAATTACTGTGGAAGACCTGAAGACAAAGGTGGAGATTACTGAGAAGAGCAAAAAGGGCAATAATATGGAACTTGATAGCGTAAAGGGGCAGCTGGAAGAAGCCAAAGAGGCTATTACGAAGTTGTTTGATGCCAATCAAATGTTGATGAAGAGCGTTGAAGATGATCCCCCGTCCTCTGCCGGATCTTCTGGGGAAGTACCAGATGAAAGTGGGAGCGTGAGAAGGAGGAGACTTTCTGAACAAGCAAAAAGAGGGTCGGAAAACATTGGGCGGTTGCAGTTACAGGTTCAGAAACTGCAGTTTCTTCTTCTGAAAATTGATGGCGAAAAAGACAGCAAAGGATCAGCTAGAATCATCGAGCGGAAGAAAAGCGTTCTTCTTCGCGACTACCTCTATGGCGTAGGAAAACCCGTCAACCAAGGGAAACGGAAGAAATCGCCCTTCTGTGCATGCATACAGCCCCCAACCAAGGGAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIP1

Protein Analysis

1795

Amino Acids

203.62

Weight (kDa)

4.98

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 14 - 87 2.9e-36 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 358
AasI GACNNNNNNGTC 1 cut(s) 2439
Acc16I TGCGCA 1 cut(s) 4189
Acc36I ACCTGC 4 cut(s) 344, 358, 1909, 2429
AccBSI CCGCTC 1 cut(s) 5266
AccI GTMKAC 1 cut(s) 1830
AccII CGCG 7 cut(s) 11, 1017, 1019, 1666, 2687, 4104, 5289
AciI CCGC 9 cut(s) 9, 409, 619, 2030, 2208, 2505, 4701, 5176, 5266
AclWI GGATC 6 cut(s) 371, 4120, 4531, 5063, 5095, 5254
AcuI CTGAAG 9 cut(s) 71, 746, 825, 882, 888, 1056, 1407, 4344, 4943
AdeI CACNNNGTG 1 cut(s) 4400
AfiI CCNNNNNNNGG 7 cut(s) 165, 1330, 1873, 3400, 4366, 5374, 5375
AflIII ACRYGT 2 cut(s) 1664, 2530
AgeI ACCGGT 1 cut(s) 4301
AjiI CACGTC 1 cut(s) 2914
AjnI CCWGG 6 cut(s) 282, 830, 1596, 3120, 3540, 4653
AjuI GAANNNNNNNTTGG 2 cut(s) 3902, 3934
AloI GAACNNNNNNTCC 4 cut(s) 1806, 1838, 3116, 3148
Alw21I GWGCWC 5 cut(s) 820, 1008, 3501, 4110, 4746
Alw44I GTGCAC 1 cut(s) 816
AlwI GGATC 6 cut(s) 371, 4120, 4531, 5063, 5095, 5254
AlwNI CAGNNNCTG 5 cut(s) 1512, 1573, 3490, 4703, 5200
AoxI GGCC 6 cut(s) 720, 754, 1332, 3174, 3738, 3813
ApaI GGGCCC 1 cut(s) 3178
ApaLI GTGCAC 1 cut(s) 816
ArsI GACNNNNNNTTYG 4 cut(s) 3683, 3715, 5144, 5176
AseI ATTAAT 2 cut(s) 203, 1176
AsiGI ACCGGT 1 cut(s) 4301
Asp700I GAANNNNTTC 3 cut(s) 309, 2961, 4144
AspLEI GCGC 8 cut(s) 195, 277, 282, 1019, 1093, 2420, 2689, 4190
AspS9I GGNCC 4 cut(s) 721, 1333, 3174, 3175
AsuC2I CCSGG 1 cut(s) 450
AsuHPI GGTGA 7 cut(s) 339, 1214, 1484, 1991, 3434, 3686, 3821
AsuII TTCGAA 2 cut(s) 881, 1700
AsuNHI GCTAGC 1 cut(s) 920
BaeGI GKGCMC 4 cut(s) 820, 988, 1324, 3178
BaeI ACNNNNGTAYC 2 cut(s) 749, 782
BanII GRGCYC 4 cut(s) 1008, 3178, 3501, 4110
BarI GAAGNNNNNNTAC 2 cut(s) 741, 773
BbsI GAAGAC 7 cut(s) 2437, 2909, 4140, 4203, 4435, 4923, 4931
Bbv12I GWGCWC 5 cut(s) 820, 1008, 3501, 4110, 4746
BceAI ACGGC 1 cut(s) 1355
BcgI CGANNNNNNTGC 6 cut(s) 143, 177, 1731, 1765, 2600, 2634
BciT130I CCWGG 6 cut(s) 284, 832, 1598, 3122, 3542, 4655
BciVI GTATCC 1 cut(s) 1156
BclI TGATCA 6 cut(s) 129, 259, 3022, 3661, 3984, 4561
BcnI CCSGG 1 cut(s) 450
BfoI RGCGCY 3 cut(s) 196, 278, 2421
BfuAI ACCTGC 4 cut(s) 344, 358, 1909, 2429
BfuI GTATCC 1 cut(s) 1156
BglI GCCNNNNNGGC 1 cut(s) 5012
BglII AGATCT 5 cut(s) 1057, 1610, 1773, 2613, 2725
BmcAI AGTACT 1 cut(s) 2874
Bme1390I CCNGG 7 cut(s) 284, 450, 832, 1598, 3122, 3542, 4655
BmgBI CACGTC 1 cut(s) 2914
BmgT120I GGNCC 4 cut(s) 721, 1333, 3174, 3175
BmiI GGNNCC 4 cut(s) 358, 3175, 3176, 4066
BmrFI CCNGG 7 cut(s) 284, 450, 832, 1598, 3122, 3542, 4655
BmrI ACTGGG 3 cut(s) 279, 692, 2215
BmtI GCTAGC 1 cut(s) 924
BmuI ACTGGG 3 cut(s) 279, 692, 2215
BoxI GACNNNNGTC 1 cut(s) 4719
BpiI GAAGAC 7 cut(s) 2437, 2909, 4140, 4203, 4435, 4923, 4931
BplI GAGNNNNNCTC 2 cut(s) 2462, 2494
BpmI CTGGAG 8 cut(s) 585, 818, 986, 1154, 1250, 1322, 1832, 3659
Bpu14I TTCGAA 2 cut(s) 881, 1700
BpuEI CTTGAG 9 cut(s) 1804, 2135, 2338, 2507, 2849, 3206, 3590, 4298, 4652
BpuMI CCSGG 1 cut(s) 450
BsaBI GATNNNNATC 5 cut(s) 1778, 2696, 3027, 4374, 5037
BsaI GGTCTC 4 cut(s) 593, 632, 3222, 4637
BsaJI CCNNGG 9 cut(s) 297, 472, 831, 1596, 2385, 3120, 3816, 5323, 5373
BsaWI WCCGGW 1 cut(s) 4301
BsaXI ACNNNNNCTCC 6 cut(s) 1806, 1836, 3557, 3587, 3908, 3938
Bsc4I CCNNNNNNNGG 7 cut(s) 165, 1330, 1873, 3400, 4366, 5374, 5375
Bse118I RCCGGY 1 cut(s) 4301
Bse3DI GCAATG 1 cut(s) 339
Bse8I GATNNNNATC 5 cut(s) 1778, 2696, 3027, 4374, 5037
BseBI CCWGG 6 cut(s) 284, 832, 1598, 3122, 3542, 4655
BseDI CCNNGG 9 cut(s) 297, 472, 831, 1596, 2385, 3120, 3816, 5323, 5373
BseJI GATNNNNATC 5 cut(s) 1778, 2696, 3027, 4374, 5037
BseLI CCNNNNNNNGG 7 cut(s) 165, 1330, 1873, 3400, 4366, 5374, 5375
BseMI GCAATG 1 cut(s) 339
BseRI GAGGAG 6 cut(s) 448, 1172, 1343, 3203, 3416, 5146
BseSI GKGCMC 4 cut(s) 820, 988, 1324, 3178
BsgI GTGCAG 3 cut(s) 1472, 2955, 3953
Bsh1236I CGCG 7 cut(s) 11, 1017, 1019, 1666, 2687, 4104, 5289
BshFI GGCC 6 cut(s) 722, 756, 1334, 3176, 3740, 3815
BshTI ACCGGT 1 cut(s) 4301
BsiHKAI GWGCWC 5 cut(s) 820, 1008, 3501, 4110, 4746
BsiSI CCGG 3 cut(s) 449, 4302, 5085
BslFI GGGAC 4 cut(s) 65, 849, 2731, 2898
BslI CCNNNNNNNGG 7 cut(s) 165, 1330, 1873, 3400, 4366, 5374, 5375
BsmBI CGTCTC 3 cut(s) 1784, 2627, 2987
BsmFI GGGAC 4 cut(s) 65, 849, 2731, 2898
BsmI GAATGC 2 cut(s) 413, 1585
BsnI GGCC 6 cut(s) 722, 756, 1334, 3176, 3740, 3815
Bso31I GGTCTC 4 cut(s) 593, 632, 3222, 4637
Bsp119I TTCGAA 2 cut(s) 881, 1700
Bsp120I GGGCCC 1 cut(s) 3174
Bsp1286I GDGCHC 8 cut(s) 820, 988, 1008, 1324, 3178, 3501, 4110, 4746
Bsp19I CCATGG 1 cut(s) 297
Bsp68I TCGCGA 2 cut(s) 4104, 5289
BspACI CCGC 9 cut(s) 9, 409, 619, 2030, 2208, 2505, 4701, 5176, 5266
BspANI GGCC 6 cut(s) 722, 756, 1334, 3176, 3740, 3815
BspFNI CGCG 7 cut(s) 11, 1017, 1019, 1666, 2687, 4104, 5289
BspLI GGNNCC 4 cut(s) 358, 3175, 3176, 4066
BspMAI CTGCAG 9 cut(s) 355, 933, 1003, 1578, 1977, 3486, 3865, 4062, 5205
BspMI ACCTGC 4 cut(s) 344, 358, 1909, 2429
BspOI GCTAGC 1 cut(s) 924
BspPI GGATC 6 cut(s) 371, 4120, 4531, 5063, 5095, 5254
BspQI GCTCTTC 5 cut(s) 1524, 2517, 3489, 4943, 5048
BspT104I TTCGAA 2 cut(s) 881, 1700
BspTNI GGTCTC 4 cut(s) 593, 632, 3222, 4637
BsrBI CCGCTC 1 cut(s) 5266
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 4301
BssAI RCCGGY 1 cut(s) 4301
BssECI CCNNGG 9 cut(s) 297, 472, 831, 1596, 2385, 3120, 3816, 5323, 5373
BssT1I CCWWGG 5 cut(s) 297, 472, 2385, 5323, 5373
Bst2UI CCWGG 6 cut(s) 284, 832, 1598, 3122, 3542, 4655
BstAPI GCANNNNNTGC 5 cut(s) 239, 1582, 3695, 3772, 3940
BstBI TTCGAA 2 cut(s) 881, 1700
BstDSI CCRYGG 1 cut(s) 297
BstENI CCTNNNNNAGG 3 cut(s) 163, 1871, 3398
BstFNI CGCG 7 cut(s) 11, 1017, 1019, 1666, 2687, 4104, 5289
BstH2I RGCGCY 3 cut(s) 196, 278, 2421
BstHHI GCGC 8 cut(s) 195, 277, 282, 1019, 1093, 2420, 2689, 4190
BstNI CCWGG 6 cut(s) 284, 832, 1598, 3122, 3542, 4655
BstNSI RCATGY 8 cut(s) 628, 2534, 2564, 2641, 3779, 4122, 4352, 5358
BstPAI GACNNNNGTC 1 cut(s) 4719
BstSCI CCNGG 7 cut(s) 282, 448, 830, 1596, 3120, 3540, 4653
BstSLI GKGCMC 4 cut(s) 820, 988, 1324, 3178
BstUI CGCG 7 cut(s) 11, 1017, 1019, 1666, 2687, 4104, 5289
BstV2I GAAGAC 7 cut(s) 2437, 2909, 4140, 4203, 4435, 4923, 4931
BstX2I RGATCY 7 cut(s) 1057, 1610, 1773, 2613, 2725, 4125, 5087
BstXI CCANNNNNNTGG 2 cut(s) 473, 2597
BstYI RGATCY 7 cut(s) 1057, 1610, 1773, 2613, 2725, 4125, 5087
BsuI GTATCC 1 cut(s) 1156
BsuRI GGCC 6 cut(s) 722, 756, 1334, 3176, 3740, 3815
BtgI CCRYGG 1 cut(s) 297
BtgZI GCGATG 1 cut(s) 4257
BtrI CACGTC 1 cut(s) 2914
BtsI GCAGTG 4 cut(s) 947, 1283, 1571, 4458
BtuMI TCGCGA 2 cut(s) 4104, 5289
BveI ACCTGC 4 cut(s) 344, 358, 1909, 2429
CaiI CAGNNNCTG 5 cut(s) 1512, 1573, 3490, 4703, 5200
CfoI GCGC 8 cut(s) 195, 277, 282, 1019, 1093, 2420, 2689, 4190
Cfr10I RCCGGY 1 cut(s) 4301
Cfr13I GGNCC 4 cut(s) 721, 1333, 3174, 3175
CseI GACGC 6 cut(s) 39, 1432, 1944, 2750, 3004, 3687
CspAI ACCGGT 1 cut(s) 4301
CspCI CAANNNNNGTGG 2 cut(s) 4030, 4065
DraIII CACNNNGTG 1 cut(s) 4400
DrdI GACNNNNNNGTC 1 cut(s) 2439
DseDI GACNNNNNNGTC 1 cut(s) 2439
Ecl136II GAGCTC 3 cut(s) 1006, 3499, 4108
Eco130I CCWWGG 5 cut(s) 297, 472, 2385, 5323, 5373
Eco24I GRGCYC 4 cut(s) 1008, 3178, 3501, 4110
Eco31I GGTCTC 4 cut(s) 593, 632, 3222, 4637
Eco32I GATATC 2 cut(s) 1125, 4569
Eco53kI GAGCTC 3 cut(s) 1006, 3499, 4108
Eco57I CTGAAG 9 cut(s) 71, 746, 825, 882, 888, 1056, 1407, 4344, 4943
EcoICRI GAGCTC 3 cut(s) 1006, 3499, 4108
EcoNI CCTNNNNNAGG 3 cut(s) 163, 1871, 3398
EcoO109I RGGNCCY 1 cut(s) 3174
EcoRI GAATTC 4 cut(s) 73, 1696, 2092, 2281
EcoRII CCWGG 6 cut(s) 282, 830, 1596, 3120, 3540, 4653
EcoRV GATATC 2 cut(s) 1125, 4569
EcoT14I CCWWGG 5 cut(s) 297, 472, 2385, 5323, 5373
EcoT22I ATGCAT 2 cut(s) 2720, 5360
EcoT38I GRGCYC 4 cut(s) 1008, 3178, 3501, 4110
ErhI CCWWGG 5 cut(s) 297, 472, 2385, 5323, 5373
Esp3I CGTCTC 3 cut(s) 1784, 2627, 2987
FalI AAGNNNNNCTT 2 cut(s) 1077, 1109
FaqI GGGAC 4 cut(s) 65, 849, 2731, 2898
FauI CCCGC 1 cut(s) 402
FbaI TGATCA 6 cut(s) 129, 259, 3022, 3661, 3984, 4561
FblI GTMKAC 1 cut(s) 1830
FriOI GRGCYC 4 cut(s) 1008, 3178, 3501, 4110
FspI TGCGCA 1 cut(s) 4189
GlaI GCGC 8 cut(s) 194, 276, 281, 1018, 1092, 2419, 2688, 4189
GsuI CTGGAG 8 cut(s) 585, 818, 986, 1154, 1250, 1322, 1832, 3659
HaeII RGCGCY 3 cut(s) 196, 278, 2421
HaeIII GGCC 6 cut(s) 722, 756, 1334, 3176, 3740, 3815
HapII CCGG 3 cut(s) 449, 4302, 5085
HgaI GACGC 6 cut(s) 39, 1432, 1944, 2750, 3004, 3687
HhaI GCGC 8 cut(s) 195, 277, 282, 1019, 1093, 2420, 2689, 4190
Hin6I GCGC 8 cut(s) 193, 275, 280, 1017, 1091, 2418, 2687, 4188
HinP1I GCGC 8 cut(s) 193, 275, 280, 1017, 1091, 2418, 2687, 4188
HincII GTYRAC 2 cut(s) 4893, 5320
HindII GTYRAC 2 cut(s) 4893, 5320
HindIII AAGCTT 1 cut(s) 876
HpaII CCGG 3 cut(s) 449, 4302, 5085
HphI GGTGA 7 cut(s) 339, 1214, 1484, 1991, 3434, 3686, 3821
Hpy166II GTNNAC 6 cut(s) 386, 818, 1831, 2683, 4893, 5320
Hpy8I GTNNAC 6 cut(s) 386, 818, 1831, 2683, 4893, 5320
Hpy99I CGWCG 3 cut(s) 16, 1341, 3703
HpyCH4IV ACGT 3 cut(s) 648, 2913, 3597
HpySE526I ACGT 3 cut(s) 648, 2913, 3597
HspAI GCGC 8 cut(s) 193, 275, 280, 1017, 1091, 2418, 2687, 4188
Ksp22I TGATCA 6 cut(s) 129, 259, 3022, 3661, 3984, 4561
LguI GCTCTTC 5 cut(s) 1524, 2517, 3489, 4943, 5048
LmnI GCTCC 7 cut(s) 451, 491, 1431, 1637, 3347, 4741, 5118
MaeII ACGT 3 cut(s) 648, 2913, 3597
MaeIII GTNAC 5 cut(s) 3162, 3389, 4493, 4649, 5184
MbiI CCGCTC 1 cut(s) 5266
MfeI CAATTG 3 cut(s) 1227, 2190, 3665
MflI RGATCY 7 cut(s) 1057, 1610, 1773, 2613, 2725, 4125, 5087
MhlI GDGCHC 8 cut(s) 820, 988, 1008, 1324, 3178, 3501, 4110, 4746
MluI ACGCGT 1 cut(s) 1664
MmeI TCCRAC 8 cut(s) 1419, 2028, 2130, 2754, 3543, 3900, 4092, 5142
Mph1103I ATGCAT 2 cut(s) 2720, 5360
MroXI GAANNNNTTC 3 cut(s) 309, 2961, 4144
MslI CAYNNNNRTG 3 cut(s) 2642, 3065, 3746
MspA1I CMGCKG 4 cut(s) 544, 4244, 4703, 4996
MspI CCGG 3 cut(s) 449, 4302, 5085
MspR9I CCNGG 7 cut(s) 284, 450, 832, 1598, 3122, 3542, 4655
MteI GCGCNGCGC 1 cut(s) 278
MunI CAATTG 3 cut(s) 1227, 2190, 3665
Mva1269I GAATGC 2 cut(s) 413, 1585
MvaI CCWGG 6 cut(s) 284, 832, 1598, 3122, 3542, 4655
MvnI CGCG 7 cut(s) 11, 1017, 1019, 1666, 2687, 4104, 5289
NciI CCSGG 1 cut(s) 450
NcoI CCATGG 1 cut(s) 297
NheI GCTAGC 1 cut(s) 920
NlaIV GGNNCC 4 cut(s) 358, 3175, 3176, 4066
NmeAIII GCCGAG 1 cut(s) 3841
NmuCI GTSAC 3 cut(s) 3389, 4493, 4649
NruI TCGCGA 2 cut(s) 4104, 5289
NsbI TGCGCA 1 cut(s) 4189
NsiI ATGCAT 2 cut(s) 2720, 5360
NspI RCATGY 8 cut(s) 628, 2534, 2564, 2641, 3779, 4122, 4352, 5358
NspV TTCGAA 2 cut(s) 881, 1700
PaeI GCATGC 4 cut(s) 628, 3779, 4352, 5358
PaqCI CACCTGC 1 cut(s) 358
PciI ACATGT 1 cut(s) 2530
PciSI GCTCTTC 5 cut(s) 1524, 2517, 3489, 4943, 5048
PcsI WCGNNNNNNNCGW 2 cut(s) 235, 1531
PctI GAATGC 2 cut(s) 413, 1585
PdmI GAANNNNTTC 3 cut(s) 309, 2961, 4144
PflFI GACNNNGTC 1 cut(s) 1688
PfoI TCCNGGA 1 cut(s) 448
PinAI ACCGGT 1 cut(s) 4301
PscI ACATGT 1 cut(s) 2530
PshAI GACNNNNGTC 1 cut(s) 4719
PshBI ATTAAT 2 cut(s) 203, 1176
Psp124BI GAGCTC 3 cut(s) 1008, 3501, 4110
Psp6I CCWGG 6 cut(s) 282, 830, 1596, 3120, 3540, 4653
PspGI CCWGG 6 cut(s) 282, 830, 1596, 3120, 3540, 4653
PspN4I GGNNCC 4 cut(s) 358, 3175, 3176, 4066
PspOMI GGGCCC 1 cut(s) 3174
PspPI GGNCC 4 cut(s) 721, 1333, 3174, 3175
PsrI GAACNNNNNNTAC 2 cut(s) 4473, 4505
PstI CTGCAG 9 cut(s) 355, 933, 1003, 1578, 1977, 3486, 3865, 4062, 5205
PstNI CAGNNNCTG 5 cut(s) 1512, 1573, 3490, 4703, 5200
PsuI RGATCY 7 cut(s) 1057, 1610, 1773, 2613, 2725, 4125, 5087
PsyI GACNNNGTC 1 cut(s) 1688
PvuII CAGCTG 3 cut(s) 544, 4244, 4996
RruI TCGCGA 2 cut(s) 4104, 5289
RseI CAYNNNNRTG 3 cut(s) 2642, 3065, 3746
SacI GAGCTC 3 cut(s) 1008, 3501, 4110
SapI GCTCTTC 5 cut(s) 1524, 2517, 3489, 4943, 5048
Sau96I GGNCC 4 cut(s) 721, 1333, 3174, 3175
SbfI CCTGCAGG 1 cut(s) 355
ScaI AGTACT 1 cut(s) 2874
ScrFI CCNGG 7 cut(s) 284, 450, 832, 1598, 3122, 3542, 4655
SdaI CCTGCAGG 1 cut(s) 355
SduI GDGCHC 8 cut(s) 820, 988, 1008, 1324, 3178, 3501, 4110, 4746
SfuI TTCGAA 2 cut(s) 881, 1700
SmiMI CAYNNNNRTG 3 cut(s) 2642, 3065, 3746
SmlI CTYRAG 9 cut(s) 1783, 2114, 2317, 2486, 2828, 3185, 3605, 4277, 4631
SmoI CTYRAG 9 cut(s) 1783, 2114, 2317, 2486, 2828, 3185, 3605, 4277, 4631
SphI GCATGC 4 cut(s) 628, 3779, 4352, 5358
Sse8387I CCTGCAGG 1 cut(s) 355
SsiI CCGC 9 cut(s) 9, 409, 619, 2030, 2208, 2505, 4701, 5176, 5266
SstI GAGCTC 3 cut(s) 1008, 3501, 4110
StyD4I CCNGG 7 cut(s) 282, 448, 830, 1596, 3120, 3540, 4653
StyI CCWWGG 5 cut(s) 297, 472, 2385, 5323, 5373
TaiI ACGT 3 cut(s) 651, 2916, 3600
TaqII GACCGA 1 cut(s) 2252
TatI WGTACW 3 cut(s) 180, 2872, 3730
TauI GCSGC 3 cut(s) 2033, 2210, 4703
TseFI GTSAC 3 cut(s) 3389, 4493, 4649
Tsp45I GTSAC 3 cut(s) 3389, 4493, 4649
TspGWI ACGGA 3 cut(s) 3141, 3601, 5347
Tth111I GACNNNGTC 1 cut(s) 1688
VneI GTGCAC 1 cut(s) 816
VspI ATTAAT 2 cut(s) 203, 1176
XagI CCTNNNNNAGG 3 cut(s) 163, 1871, 3398
XbaI TCTAGA 3 cut(s) 26, 1114, 2173
XceI RCATGY 8 cut(s) 628, 2534, 2564, 2641, 3779, 4122, 4352, 5358
XcmI CCANNNNNNNNNTGG 1 cut(s) 1297
XmiI GTMKAC 1 cut(s) 1830
XmnI GAANNNNTTC 3 cut(s) 309, 2961, 4144
ZrmI AGTACT 1 cut(s) 2874
Zsp2I ATGCAT 2 cut(s) 2720, 5360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.