Rroxscaffold_7G00177160

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005688
Physical Location & Seq
Forward (+)
16693590 .. 16700738
7149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00177160.1

Sequence Viewer

Length: 5481 bp
ATGGGGTGTGACCTTGTGAGCACAAATTCATCTGAGAATTACAGATTTGGTGGGTTGAAGCCTGTCCCGGTTGAGTTTACTCGTCGGTTTTGGGTTACTGCAACACCAGTATCGACGGTGGTGGTGATATTGGCCGGAGTTATGGCGACTGTGTCCCAAGCAGATTCTAGACGCATGTATTCTTGGTGGTGGGACAGCCACATAAGCCCAAAGAACTCGAGATGGCTGAAGGAAAATCTTACAGATATGGATGCCAAAGTCAAACACATGATCAAACTCATTGAAGAAGATGCAGATTCCTTTGCTAGAAGGGCAGAAATGTATTACAAAAAACGCCCAGAACTAATGAAATTGGTTGAAGAGTTCTACCGAGCATACCGTGCGTTAGCTGAAAGATATGATCATGCAACTGGGGCTCTGCGCCAGGCTCATCGAACCATGGCAGAGGCATTTCCCAACCAAGTTCCGTTTGTAGATGACTCGCCTGCAGGTTCTTCTGCTAGTGAGACTGATCCCCGAACACCTGAGATGCCAGCTCCTATACGAGCATTATTTGACCTTGATGAATTGCAAAAAGATGCTCTGGGACTCTCCTCCTCAACCCATTTCCATACTCTCAAAAGGAATGGAGCTTTTAGTGAAGAATCTGACTCTGGAACCAGCCGAATTGGTTTGAAACAGCTGAATGATCTCCTTGGATCGGGAGAAGGAAGGGCGAAAAAGGGCCTCAACTTCCTTGATGCTGACGCAAAAGAACACAGCATGCAGAGCAATGGCCATGATCTTAAAAACCGAGCCTTGTTGGGGGATGATCAAGTGGGCAAAGCAGAGACAGAAATATCAAACTTAAAGAAAGCTCTTGCTAAACTAGAAGCTGAAAAGGAAGCTGGCTTACTTCAGTACCAAGAGTGTTTAGAGAGAGTATCTAATTTGGAGTCTGAAGTCTCTCGTGCACAAGAGGATTCCAGGGGACTTAATGAACGAGCCAGTAAAGCTGAAGCTGAAGTTCAAACTGCAAAGGAAGCACTTAACAAATTAGAGGCTGAAAGGGAAGCTAGTCTTCTTCAGTATCAGGAGTGCTTGGACAAAATCTCTAATCTGGAAAGCATCATCTCTTGTGCCCAAAAGGATGCTGGAGAGCTAAATGATCGAGCTAGTAGAGCTGAATTTGCATCTGAATCTCTACAGAAAGATCTTGAAAGGGTGGCATCTGAGAAAGAAGCTGCACTTGCACAATACGAACGGTGTCTGGAGAATATATCAAATCTGGAGGACAAACTACTGCATGTTGAGGAGGATGCCAAGAGGATTAACGAGCGTGCTTTTAAAGCTGAACTCGAAGTTGAATCCTTGAAGCAAGCAATTGCCAATTTAACTGAAGAGAAGGAAGCTGCTGCTCTCCAGTACAAGCAATGCCTGGAAATGATATCTAATCTTGAGCATAAGATCTCTCATGCGGAGGAAGAGGCACTACGGCTACATTCTCAGATAGACGACGGGATTGCGAAGTTAAAGGATTCGGAAGAAAAGTGTCTTCTGCTGGTAAACTCAAATCAGAATCTGCAGTCGGAGTGGGAGTCTGTGGTGAAGCAAATGCAATCTCAAGGTGAAGAGCTTACAGAGAAGCAGAAGGAGTTGGGTAGACTTTGGGCTTGCATACAAGAAGAGCGGTTGCGATTTATGGAGGCTGAAACTGCTTTCCAGACACTGCAGCATTTGCATTCCCAGTCCCAGGAGGAACTTAGATCTCTTGTAGCTGAGCTCCAGAATAGAGATTTGATTCTGAAGGACATGGAAGCACGAAGTCAGAGTTTGGATGATCAAGTCCAGAAGGTGAAGGAGGAAAACAAGAGCCTAAGTGAGATCAATTTGTCTTCATCTGTTTCAATAAAAGATCTGCAAGGTGAGATCTTAATCTTGAGGGAGACGATTAAGAAACTTGAAGATGAAGTTGAACTTCGAGTGGACCAAAGAAATGCTCTTCAGCAAGAGATATACTGTCTGAAAGAGGAACTCAGTGACCTGAACAAGAAACACCAAGCTATGCTAGAGCAGGTGGAGTCAGTTGGCTTGGATCCAGTGTGCATCAGGTCATCTGTGAAGGAGTTGCAAGATGAGAACTTACAGCTCAAACAAACATGTGAGGCTGATAAAAGTGAGAAAGTGGCACTGTTGGAAAAGTTGGAAATCATGCAGAAACTTCGGGAGAAAAATGTTCTTTTGGAGAATTCCCTTTCTGATTTGAATGTTGAGTTAGAAGGGGTCAGAGGGAAGGTAAAGGAGTTGGAACAATCATGTCAGTCTCTTCTGGCAGAAAAAGGTACTCTTCTTGCTGAAAATGGCACTCTGATTTCCCAGTTGCAGATTATGACTGAGAATTTAGAAAAATCATCAGAGAAGAACAAATTTTTGGAGAATTCTCTCTTTGATGCGAATGCTGAACTGGAAGGCTTGAGTGTAAAATCAAAGAGCTTAGAAGAATCATGCCTACTGCTTGGTAATGATAAGACTGGTCTGATCACTGAGAGAGAGAGTTTAATTTTCGAGTTGGGCAGTACTCGGCAAAGACTGGAAGATCTGGAAAAAGGATATGCAGAGATAGAAGAGAAACTCTCTGTTCTGGAGAAGGAAAGAGATTCTGCACTTTGTAAAGTGGAAGAGCTAAATGTATGCTTAGATTCTGAGAAGCAAAATCATGCCAGTTCTGTTGAGTTGAGGGAAACCCAGTTGGCTGAAATGGAACTGAAGATCTCTCATCTCCAAGCAGAAGGGATATGCAGGAAGAAAGAATTTGAAGAGGAACAAGACAAATCTGTTACTGCACAGATAGAAATCTTTGTCCTGCAGAAATGTGTAGAAGATTTGGAAGAGAAGAACTTATCCCTCATGATTGAGCGTCAAAAGCTTTTGGGGGCATCCGCAATGTCAGAGAAGCTGATTTCTGTCCTAGAGCATGGCAAACTTGAACAACAGAAGGAAATCAAGTCATTGTTTGTGCAAATTAAAGCACTGAGAATGGGGCTGTATCAGGTGTTGAAGAATGTTGACATTGATGCAAACCTTGGGTGTGCACAAAAGGATGAGCAAGACCAAGCACTCCTTAATCATATACTTATCAAACTTCAAGACAAACAAAATTCTTTCTCTGAAAGTTGTGATGAAAACCAGCGGTTGCTTATTGAGAACTCTGTTCTCATAGCAATGCTTGCCCAAATGAAACTAGAGGCGGACTGTTTTATGAGGGAAAGAAACACTCTTGACCATGAGTTCAGGACCCAGTCTGAGAAGTTCTTGGTGTTGCAGAGTGGCGCCCAAAGGCTTCGTGACATGAATGCAGAATTGAATTTGAAGGTAGTGGAGGGAGAACACAGAGAGGGGGTTTTGAGGACTGAAATAGATAATCTGCACGAACAGTTGCTGGACTTGCAAAGCATTTACAAGAGTCTACAAAAAGAAAATTCCCAGATGGTTGAAGATAAAGGATCCTTGAAAAGGACGGTCTTGAATTTGGAGGAAGAGGCGCGTAACCTAGAAGAGGATAAATGCGTCATGTTTGCTGAAACAATATATTATAGTAACCTCTCCCTTGTTTTCGATGATATTATTTACCAAAAGCAACTGGAACTGGAAGAGCTGAGTCACAATTATGATGAACTTCATCTTGGCAATAATGACCTGAAAGAGAAGGAGTCATTGAATAAGTCTGAGGATGAGCTGAACTTGGTTAAATCTGTGAATGATCAATTGAATGGTGATATTGCAAATGCAAAGGATGGTCTGTCTCAAAAGGAAAATGAGCTTTTGGAAGCCGGGCAGATCATAAATGAACTACAGAATGAGAAAAAAGAGCTGTATATACTGGTGGAGGATTTGACTAGTAAAAGTGATAATGCGAAGGTGGTGCTGGAAGATCAAGAGAAGCAAATTTTCAAACTACATGATGACAATGACCGTCATAGTAAAGAGATTGGATGCCTCCGTGAAGTGAATCAGAAGTTGGAGGTGGAACTATCAAAATTGCATGAAGAAGCTGAAAAAGTCAAAATTGAGGAGGAAAGGTTGGTCAGTGAACTAAAAAGGGGAAGAGAGGAGATAGAAATGTGGGTGACTCACGCAGCCACCCTCTTTGGAGAACTGCAGATCTCCTCCATTCGTGAAACATTGTTTGAAGGAAAGATCCGCGAGCTCATTGAAGCCTGTCAGATCCTTGAAAACAAAAGTGTTTCCAAAGGTCTGGAGAGTGAACAGATGAAAGAAAGAGTCGGCACATTGGAACATGAAAATGGAGAGCTACAAGCTCAGTTGGCTGCCTATATTCCAGCTGTCATCTCTCTGAAGGAGTGTACAACAGCCCTTGAGAATCATTCACTTATTAACACCAGATGTCATGAACTTGACACTAAAGCCTCAGAGGATGCCCTTCTGCAAGCTGAGAGCTCTCAAACGGATGGACATCAAATTGACAGAGTGTCAGATGGCATTTCAGGACTGCAGGATTTACAAAGAAGAATCAAAGCTATTGAAAAGGCTATGGTAGAAAAGGAAAGCCATCTTACTGCCAATCAGGAAGGTAAACAATTTGGGGACAGGTTCGGTACTGAGAAGCCTGAAATCTCTGAAGCGGGGAACGAAGTTCTGACAAAGGACATCATTCTCGATCAGATATCAGAGTGTTCATCCTATGGGGTAAGCAGGAGAGAAACCACAGAGCCTGATGCTCAGATGATGGAGTTATGGGAGACCACTGAACAGGATGGCAGCATTGATTTGATGGTTGGCAAGGCCCAGAAGGCGACTACTGTACCGACTGACCACAGTCAGACTGAAGCTATAAAGAAACATAAGAATAAATATCCCTCTTCAGAATCATTGGTTGAGAAAGAATATAGCGTTGACAAATTAGAGATCTCTAAGAGATTCTCCGAGCCCCGTCAAGAAGGCAACAAGAGAAGGATTCTAGAAAGACTTGATTCTGATGTGCAAAAGTTGACAAACCTTCAAATTACCGTGGAAGACCTGAAGAAGAAGGTGGACATTACTGAGAAGACCAAAAAGGGCAAAGGTATTGAATTTGGTACTGTAAAAGAGCAGCTGGATGAAGCCGAAGAAGCCATCACTAGGTTGTTTGATGCCAATAACAAATTGATGAAGAGTGTCGAAGATGAATTTGTGTCTCCTGATGGAGCTTCTGAGATAGTCCCAGATCACAGTGGGAGTGTCAGCAGAAGGAGGCTGTCCGAACAAGCAAAAAGAGGGTCAGAGAAAATCGGTCGATTGCAGTTGGAGGTACAGAAACTGCAATTTCTTCTACTGAAGCTGGATGGTGAAAAGGAAAGCAAAGGGTCAACCAGGATCAAGGAGAGGAAAACAAGAGTTCTTCTGCGGGACTATCTCTATTCTGGCACTAGAATGAGCAACACAACCCCAAAGCGTAAGAAAGCTCCTTTTTGTGCATGCATGCCCCCAACTAAGGGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIP1

Protein Analysis

1826

Amino Acids

207.61

Weight (kDa)

4.99

Isoelectric Point (pI)

52.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 61 - 134 1.7e-37 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2044
Acc36I ACCTGC 2 cut(s) 479, 2044
AccB1I GGYRCC 1 cut(s) 3308
AccBSI CCGCTC 1 cut(s) 1669
AccI GTMKAC 2 cut(s) 1642, 3445
AccII CGCG 2 cut(s) 3523, 4182
AciI CCGC 8 cut(s) 1457, 1669, 2919, 3169, 3227, 4180, 4622, 5383
AclWI GGATC 9 cut(s) 506, 706, 2069, 2082, 3477, 3490, 4171, 4198, 5360
AcoI YGGCCR 2 cut(s) 132, 775
AcyI GRCGYC 1 cut(s) 3309
AfaI GTAC 9 cut(s) 902, 1406, 2323, 2557, 4345, 4597, 4803, 5077, 5289
AfiI CCNNNNNNNGG 8 cut(s) 700, 804, 1732, 3492, 3535, 5118, 5470, 5471
AflIII ACRYGT 1 cut(s) 2138
AhdI GACNNNNNGTC 1 cut(s) 4468
AhlI ACTAGT 1 cut(s) 3875
AjnI CCWGG 5 cut(s) 423, 965, 1416, 1731, 5348
AjuI GAANNNNNNNTTGG 6 cut(s) 2393, 2425, 3645, 3677, 3980, 4012
Alw21I GWGCWC 6 cut(s) 23, 955, 1764, 3073, 4188, 4439
Alw26I GTCTC 8 cut(s) 500, 824, 949, 1919, 2307, 3786, 4733, 5178
Alw44I GTGCAC 2 cut(s) 951, 3069
AlwI GGATC 9 cut(s) 506, 706, 2069, 2082, 3477, 3490, 4171, 4198, 5360
AlwNI CAGNNNCTG 5 cut(s) 1561, 1708, 3418, 4712, 5296
Ama87I CYCGRG 1 cut(s) 217
AoxI GGCC 4 cut(s) 132, 724, 775, 4782
ApaLI GTGCAC 2 cut(s) 951, 3069
ApeKI GCWGC 8 cut(s) 1223, 1391, 1394, 1711, 4115, 4307, 4758, 5089
ArsI GACNNNNNNTTYG 2 cut(s) 5240, 5272
AspLEI GCGC 3 cut(s) 423, 3311, 3523
AspS9I GGNCC 4 cut(s) 724, 1966, 3273, 4783
AsuC2I CCSGG 2 cut(s) 68, 3811
AsuHPI GGTGA 8 cut(s) 136, 1597, 1619, 1846, 1916, 3764, 4117, 5336
AvaI CYCGRG 1 cut(s) 217
AvaII GGWCC 2 cut(s) 1966, 3273
BaeGI GKGCMC 3 cut(s) 955, 1123, 3073
BaeI ACNNNNGTAYC 6 cut(s) 93, 126, 884, 917, 4785, 4818
BalI TGGCCA 1 cut(s) 777
BamHI GGATCC 2 cut(s) 2074, 3482
BanI GGYRCC 1 cut(s) 3308
BanII GRGCYC 5 cut(s) 418, 1764, 4188, 4439, 4929
BarI GAAGNNNNNNTAC 4 cut(s) 876, 908, 1455, 1487
BauI CACGAG 1 cut(s) 948
BbsI GAAGAC 5 cut(s) 1052, 1526, 1866, 5019, 5051
Bbv12I GWGCWC 6 cut(s) 23, 955, 1764, 3073, 4188, 4439
BbvI GCAGC 8 cut(s) 1210, 1378, 1381, 1723, 4127, 4294, 4770, 5101
BceAI ACGGC 1 cut(s) 1490
BcgI CGANNNNNNTGC 6 cut(s) 1484, 1518, 2183, 2217, 3883, 3917
BciT130I CCWGG 5 cut(s) 425, 967, 1418, 1733, 5350
BclI TGATCA 6 cut(s) 270, 400, 811, 1819, 2517, 3739
BcnI CCSGG 2 cut(s) 68, 3811
BcoDI GTCTC 8 cut(s) 500, 824, 949, 1919, 2307, 3786, 4733, 5178
BcuI ACTAGT 1 cut(s) 3875
BfmI CTRYAG 8 cut(s) 486, 1184, 1562, 1709, 2843, 3830, 4136, 4490
BfoI RGCGCY 1 cut(s) 3312
BfuAI ACCTGC 2 cut(s) 479, 2044
BglI GCCNNNNNGGC 1 cut(s) 4790
BglII AGATCT 9 cut(s) 1192, 1446, 1745, 1894, 1908, 2575, 2748, 4140, 4905
BisI GCNGC 8 cut(s) 1224, 1392, 1395, 1712, 4116, 4308, 4759, 5090
BlpI GCTNAGC 1 cut(s) 1758
BlsI GCNGC 8 cut(s) 1225, 1393, 1396, 1713, 4117, 4309, 4760, 5091
BmcAI AGTACT 1 cut(s) 2557
Bme1390I CCNGG 7 cut(s) 68, 425, 967, 1418, 1733, 3811, 5350
Bme18I GGWCC 2 cut(s) 1966, 3273
BmeRI GACNNNNNGTC 1 cut(s) 4468
BmeT110I CYCGRG 1 cut(s) 217
BmgT120I GGNCC 4 cut(s) 724, 1966, 3273, 4783
BmiI GGNNCC 5 cut(s) 658, 2076, 3275, 3310, 3484
BmrFI CCNGG 7 cut(s) 68, 425, 967, 1418, 1733, 3811, 5350
BmrI ACTGGG 5 cut(s) 420, 1720, 2350, 2719, 3271
BmuI ACTGGG 5 cut(s) 420, 1720, 2350, 2719, 3271
BoxI GACNNNNGTC 1 cut(s) 4815
BpiI GAAGAC 5 cut(s) 1052, 1526, 1866, 5019, 5051
BplI GAGNNNNNCTC 2 cut(s) 2597, 2629
BpmI CTGGAG 7 cut(s) 1155, 1271, 1289, 1385, 1748, 2642, 4256
Bpu1102I GCTNAGC 1 cut(s) 1758
BpuEI CTTGAG 5 cut(s) 1457, 1587, 1939, 2473, 4376
BpuMI CCSGG 2 cut(s) 68, 3811
BsaBI GATNNNNATC 3 cut(s) 1913, 2831, 4452
BsaHI GRCGYC 1 cut(s) 3309
BsaI GGTCTC 1 cut(s) 4733
BsaJI CCNNGG 6 cut(s) 438, 694, 966, 1731, 3061, 5007
BsaXI ACNNNNNCTCC 4 cut(s) 1562, 1592, 4043, 4073
Bsc4I CCNNNNNNNGG 8 cut(s) 700, 804, 1732, 3492, 3535, 5118, 5470, 5471
Bse3DI GCAATG 4 cut(s) 778, 1418, 2928, 3207
Bse8I GATNNNNATC 3 cut(s) 1913, 2831, 4452
BseBI CCWGG 5 cut(s) 425, 967, 1418, 1733, 5350
BseDI CCNNGG 6 cut(s) 438, 694, 966, 1731, 3061, 5007
BseJI GATNNNNATC 3 cut(s) 1913, 2831, 4452
BseLI CCNNNNNNNGG 8 cut(s) 700, 804, 1732, 3492, 3535, 5118, 5470, 5471
BseMI GCAATG 4 cut(s) 778, 1418, 2928, 3207
BseRI GAGGAG 6 cut(s) 583, 586, 1307, 4064, 4103, 4135
BseSI GKGCMC 3 cut(s) 955, 1123, 3073
BseXI GCAGC 8 cut(s) 1210, 1378, 1381, 1723, 4127, 4294, 4770, 5101
BsgI GTGCAG 4 cut(s) 1209, 2625, 2805, 3389
Bsh1236I CGCG 2 cut(s) 3523, 4182
Bsh1285I CGRYCG 1 cut(s) 5272
BshFI GGCC 4 cut(s) 134, 726, 777, 4784
BshNI GGYRCC 1 cut(s) 3308
BsiEI CGRYCG 1 cut(s) 5272
BsiHKAI GWGCWC 6 cut(s) 23, 955, 1764, 3073, 4188, 4439
BsiHKCI CYCGRG 1 cut(s) 217
BsiSI CCGG 3 cut(s) 68, 135, 3810
BslFI GGGAC 9 cut(s) 50, 139, 206, 600, 984, 1714, 4598, 5183, 5399
BslI CCNNNNNNNGG 8 cut(s) 700, 804, 1732, 3492, 3535, 5118, 5470, 5471
BsmAI GTCTC 8 cut(s) 500, 824, 949, 1919, 2307, 3786, 4733, 5178
BsmBI CGTCTC 1 cut(s) 1919
BsmFI GGGAC 9 cut(s) 50, 139, 206, 600, 984, 1714, 4598, 5183, 5399
BsmI GAATGC 3 cut(s) 1720, 2440, 3337
BsnI GGCC 4 cut(s) 134, 726, 777, 4784
Bso31I GGTCTC 1 cut(s) 4733
BsoBI CYCGRG 1 cut(s) 217
Bsp1286I GDGCHC 9 cut(s) 23, 418, 955, 1123, 1764, 3073, 4188, 4439, 4929
Bsp1407I TGTACA 1 cut(s) 4343
Bsp1720I GCTNAGC 1 cut(s) 1758
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 8 cut(s) 1457, 1669, 2919, 3169, 3227, 4180, 4622, 5383
BspANI GGCC 4 cut(s) 134, 726, 777, 4784
BspFNI CGCG 2 cut(s) 3523, 4182
BspHI TCATGA 2 cut(s) 2886, 4387
BspLI GGNNCC 5 cut(s) 658, 2076, 3275, 3310, 3484
BspMAI CTGCAG 6 cut(s) 490, 1566, 1713, 2847, 4140, 4494
BspMI ACCTGC 2 cut(s) 479, 2044
BspPI GGATC 9 cut(s) 506, 706, 2069, 2082, 3477, 3490, 4171, 4198, 5360
BspQI GCTCTTC 5 cut(s) 1605, 1659, 1986, 2652, 3624
BspT107I GGYRCC 1 cut(s) 3308
BspTNI GGTCTC 1 cut(s) 4733
BsrBI CCGCTC 1 cut(s) 1669
BsrDI GCAATG 4 cut(s) 778, 1418, 2928, 3207
BsrGI TGTACA 1 cut(s) 4343
BssECI CCNNGG 6 cut(s) 438, 694, 966, 1731, 3061, 5007
BssNI GRCGYC 1 cut(s) 3309
BssSI CACGAG 1 cut(s) 948
BssT1I CCWWGG 3 cut(s) 438, 694, 3061
Bst2BI CACGAG 1 cut(s) 948
Bst2UI CCWGG 5 cut(s) 425, 967, 1418, 1733, 5350
BstACI GRCGYC 1 cut(s) 3309
BstAPI GCANNNNNTGC 3 cut(s) 380, 1717, 3206
BstAUI TGTACA 1 cut(s) 4343
BstDSI CCRYGG 2 cut(s) 438, 5007
BstENI CCTNNNNNAGG 2 cut(s) 3490, 3533
BstFNI CGCG 2 cut(s) 3523, 4182
BstH2I RGCGCY 1 cut(s) 3312
BstHHI GCGC 3 cut(s) 423, 3311, 3523
BstMAI GTCTC 8 cut(s) 500, 824, 949, 1919, 2307, 3786, 4733, 5178
BstMCI CGRYCG 1 cut(s) 5272
BstNI CCWGG 5 cut(s) 425, 967, 1418, 1733, 5350
BstNSI RCATGY 6 cut(s) 178, 766, 1289, 2142, 5457, 5461
BstPAI GACNNNNGTC 1 cut(s) 4815
BstSCI CCNGG 7 cut(s) 66, 423, 965, 1416, 1731, 3809, 5348
BstSFI CTRYAG 8 cut(s) 486, 1184, 1562, 1709, 2843, 3830, 4136, 4490
BstSLI GKGCMC 3 cut(s) 955, 1123, 3073
BstUI CGCG 2 cut(s) 3523, 4182
BstV1I GCAGC 8 cut(s) 1210, 1378, 1381, 1723, 4127, 4294, 4770, 5101
BstV2I GAAGAC 5 cut(s) 1052, 1526, 1866, 5019, 5051
BstXI CCANNNNNNTGG 1 cut(s) 4234
BsuRI GGCC 4 cut(s) 134, 726, 777, 4784
BtgI CCRYGG 2 cut(s) 438, 5007
BtsI GCAGTG 1 cut(s) 1706
BtsIMutI CAGTG 9 cut(s) 1706, 2023, 2085, 2168, 2520, 3008, 4072, 4743, 5215
BveI ACCTGC 2 cut(s) 479, 2044
CaiI CAGNNNCTG 5 cut(s) 1561, 1708, 3418, 4712, 5296
CciI TCATGA 2 cut(s) 2886, 4387
CfoI GCGC 3 cut(s) 423, 3311, 3523
Cfr13I GGNCC 4 cut(s) 724, 1966, 3273, 4783
CseI GACGC 4 cut(s) 180, 755, 2885, 3535
Csp6I GTAC 9 cut(s) 901, 1405, 2322, 2556, 4344, 4596, 4802, 5076, 5288
CspCI CAANNNNNGTGG 2 cut(s) 4108, 4143
CviQI GTAC 9 cut(s) 901, 1405, 2322, 2556, 4344, 4596, 4802, 5076, 5288
DinI GGCGCC 1 cut(s) 3310
DraI TTTAAA 1 cut(s) 1327
DriI GACNNNNNGTC 1 cut(s) 4468
EaeI YGGCCR 2 cut(s) 132, 775
Eam1105I GACNNNNNGTC 1 cut(s) 4468
EciI GGCGGA 1 cut(s) 3242
Ecl136II GAGCTC 3 cut(s) 1762, 4186, 4437
Eco130I CCWWGG 3 cut(s) 438, 694, 3061
Eco24I GRGCYC 5 cut(s) 418, 1764, 4188, 4439, 4929
Eco31I GGTCTC 1 cut(s) 4733
Eco32I GATATC 2 cut(s) 1428, 4665
Eco47I GGWCC 2 cut(s) 1966, 3273
Eco53kI GAGCTC 3 cut(s) 1762, 4186, 4437
Eco88I CYCGRG 1 cut(s) 217
EcoICRI GAGCTC 3 cut(s) 1762, 4186, 4437
EcoNI CCTNNNNNAGG 2 cut(s) 3490, 3533
EcoO109I RGGNCCY 2 cut(s) 724, 3273
EcoRI GAATTC 2 cut(s) 2227, 2416
EcoRII CCWGG 5 cut(s) 423, 965, 1416, 1731, 5348
EcoRV GATATC 2 cut(s) 1428, 4665
EcoT14I CCWWGG 3 cut(s) 438, 694, 3061
EcoT22I ATGCAT 1 cut(s) 5459
EcoT38I GRGCYC 5 cut(s) 418, 1764, 4188, 4439, 4929
EgeI GGCGCC 1 cut(s) 3310
EheI GGCGCC 1 cut(s) 3310
ErhI CCWWGG 3 cut(s) 438, 694, 3061
Esp3I CGTCTC 1 cut(s) 1919
FaqI GGGAC 9 cut(s) 50, 139, 206, 600, 984, 1714, 4598, 5183, 5399
FauI CCCGC 2 cut(s) 4615, 5376
FbaI TGATCA 6 cut(s) 270, 400, 811, 1819, 2517, 3739
FblI GTMKAC 2 cut(s) 1642, 3445
Fnu4HI GCNGC 8 cut(s) 1224, 1392, 1395, 1712, 4116, 4308, 4759, 5090
FriOI GRGCYC 5 cut(s) 418, 1764, 4188, 4439, 4929
Fsp4HI GCNGC 8 cut(s) 1224, 1392, 1395, 1712, 4116, 4308, 4759, 5090
GlaI GCGC 3 cut(s) 422, 3310, 3522
GluI GCNGC 8 cut(s) 1224, 1392, 1395, 1712, 4116, 4308, 4759, 5090
GsuI CTGGAG 7 cut(s) 1155, 1271, 1289, 1385, 1748, 2642, 4256
HaeII RGCGCY 1 cut(s) 3312
HaeIII GGCC 4 cut(s) 134, 726, 777, 4784
HapII CCGG 3 cut(s) 68, 135, 3810
HgaI GACGC 4 cut(s) 180, 755, 2885, 3535
HhaI GCGC 3 cut(s) 423, 3311, 3523
Hin1I GRCGYC 1 cut(s) 3309
Hin6I GCGC 3 cut(s) 421, 3309, 3521
HinP1I GCGC 3 cut(s) 421, 3309, 3521
HincII GTYRAC 4 cut(s) 3046, 4894, 4989, 5346
HindII GTYRAC 4 cut(s) 3046, 4894, 4989, 5346
HindIII AAGCTT 1 cut(s) 2903
HpaII CCGG 3 cut(s) 68, 135, 3810
HphI GGTGA 8 cut(s) 136, 1597, 1619, 1846, 1916, 3764, 4117, 5336
Hpy99I CGWCG 3 cut(s) 87, 118, 1499
Hsp92I GRCGYC 1 cut(s) 3309
HspAI GCGC 3 cut(s) 421, 3309, 3521
KasI GGCGCC 1 cut(s) 3308
Ksp22I TGATCA 6 cut(s) 270, 400, 811, 1819, 2517, 3739
LguI GCTCTTC 5 cut(s) 1605, 1659, 1986, 2652, 3624
LmnI GCTCC 5 cut(s) 541, 629, 1767, 5183, 5446
Lsp1109I GCAGC 8 cut(s) 1210, 1378, 1381, 1723, 4127, 4294, 4770, 5101
MaeIII GTNAC 9 cut(s) 8, 94, 2018, 2815, 3323, 3524, 3575, 3638, 4105
MbiI CCGCTC 1 cut(s) 1669
MfeI CAATTG 2 cut(s) 1362, 3743
MhlI GDGCHC 9 cut(s) 23, 418, 955, 1123, 1764, 3073, 4188, 4439, 4929
MlsI TGGCCA 1 cut(s) 777
MluNI TGGCCA 1 cut(s) 777
Mly113I GGCGCC 1 cut(s) 3309
MmeI TCCRAC 6 cut(s) 1548, 2154, 2163, 2265, 3978, 5262
Mox20I TGGCCA 1 cut(s) 777
Mph1103I ATGCAT 1 cut(s) 5459
MscI TGGCCA 1 cut(s) 777
MslI CAYNNNNRTG 4 cut(s) 3200, 3645, 4281, 5408
Msp20I TGGCCA 1 cut(s) 777
MspA1I CMGCKG 4 cut(s) 682, 3169, 4322, 5092
MspI CCGG 3 cut(s) 68, 135, 3810
MspR9I CCNGG 7 cut(s) 68, 425, 967, 1418, 1733, 3811, 5350
MunI CAATTG 2 cut(s) 1362, 3743
Mva1269I GAATGC 3 cut(s) 1720, 2440, 3337
MvaI CCWGG 5 cut(s) 425, 967, 1418, 1733, 5350
MvnI CGCG 2 cut(s) 3523, 4182
NarI GGCGCC 1 cut(s) 3309
NciI CCSGG 2 cut(s) 68, 3811
NcoI CCATGG 1 cut(s) 438
NlaIV GGNNCC 5 cut(s) 658, 2076, 3275, 3310, 3484
NmeAIII GCCGAG 1 cut(s) 2539
NmuCI GTSAC 5 cut(s) 8, 2018, 3323, 3638, 4105
NsiI ATGCAT 1 cut(s) 5459
NspI RCATGY 6 cut(s) 178, 766, 1289, 2142, 5457, 5461
PaeI GCATGC 3 cut(s) 766, 5457, 5461
PaeR7I CTCGAG 1 cut(s) 217
PagI TCATGA 2 cut(s) 2886, 4387
PaqCI CACCTGC 1 cut(s) 2044
PciI ACATGT 1 cut(s) 2138
PciSI GCTCTTC 5 cut(s) 1605, 1659, 1986, 2652, 3624
PctI GAATGC 3 cut(s) 1720, 2440, 3337
PflFI GACNNNGTC 2 cut(s) 151, 3277
PkrI GCNGC 8 cut(s) 1225, 1393, 1396, 1713, 4117, 4309, 4760, 5091
PluTI GGCGCC 1 cut(s) 3312
PpuMI RGGWCCY 1 cut(s) 3273
PscI ACATGT 1 cut(s) 2138
PshAI GACNNNNGTC 1 cut(s) 4815
Psp124BI GAGCTC 3 cut(s) 1764, 4188, 4439
Psp5II RGGWCCY 1 cut(s) 3273
Psp6I CCWGG 5 cut(s) 423, 965, 1416, 1731, 5348
PspGI CCWGG 5 cut(s) 423, 965, 1416, 1731, 5348
PspN4I GGNNCC 5 cut(s) 658, 2076, 3275, 3310, 3484
PspPI GGNCC 4 cut(s) 724, 1966, 3273, 4783
PspPPI RGGWCCY 1 cut(s) 3273
PstI CTGCAG 6 cut(s) 490, 1566, 1713, 2847, 4140, 4494
PstNI CAGNNNCTG 5 cut(s) 1561, 1708, 3418, 4712, 5296
PsyI GACNNNGTC 2 cut(s) 151, 3277
PvuII CAGCTG 3 cut(s) 682, 4322, 5092
RsaI GTAC 9 cut(s) 902, 1406, 2323, 2557, 4345, 4597, 4803, 5077, 5289
RsaNI GTAC 9 cut(s) 901, 1405, 2322, 2556, 4344, 4596, 4802, 5076, 5288
RseI CAYNNNNRTG 4 cut(s) 3200, 3645, 4281, 5408
SacI GAGCTC 3 cut(s) 1764, 4188, 4439
SapI GCTCTTC 5 cut(s) 1605, 1659, 1986, 2652, 3624
SatI GCNGC 8 cut(s) 1224, 1392, 1395, 1712, 4116, 4308, 4759, 5090
Sau96I GGNCC 4 cut(s) 724, 1966, 3273, 4783
SbfI CCTGCAGG 1 cut(s) 490
ScaI AGTACT 1 cut(s) 2557
ScrFI CCNGG 7 cut(s) 68, 425, 967, 1418, 1733, 3811, 5350
SdaI CCTGCAGG 1 cut(s) 490
SduI GDGCHC 9 cut(s) 23, 418, 955, 1123, 1764, 3073, 4188, 4439, 4929
SfcI CTRYAG 8 cut(s) 486, 1184, 1562, 1709, 2843, 3830, 4136, 4490
SfoI GGCGCC 1 cut(s) 3310
Sfr274I CTCGAG 1 cut(s) 217
SinI GGWCC 2 cut(s) 1966, 3273
SlaI CTCGAG 1 cut(s) 217
SmiMI CAYNNNNRTG 4 cut(s) 3200, 3645, 4281, 5408
SmlI CTYRAG 6 cut(s) 217, 1436, 1602, 1918, 2452, 4355
SmoI CTYRAG 6 cut(s) 217, 1436, 1602, 1918, 2452, 4355
SpeI ACTAGT 1 cut(s) 3875
SphI GCATGC 3 cut(s) 766, 5457, 5461
Sse8387I CCTGCAGG 1 cut(s) 490
SsiI CCGC 8 cut(s) 1457, 1669, 2919, 3169, 3227, 4180, 4622, 5383
SspDI GGCGCC 1 cut(s) 3308
SstI GAGCTC 3 cut(s) 1764, 4188, 4439
StyD4I CCNGG 7 cut(s) 66, 423, 965, 1416, 1731, 3809, 5348
StyI CCWWGG 3 cut(s) 438, 694, 3061
TaqII GACCGA 1 cut(s) 5258
TatI WGTACW 3 cut(s) 1404, 2555, 4343
TscAI CASTG 9 cut(s) 1713, 2023, 2085, 2175, 2527, 3015, 4072, 4750, 5215
TseFI GTSAC 5 cut(s) 8, 2018, 3323, 3638, 4105
TseI GCWGC 8 cut(s) 1223, 1391, 1394, 1711, 4115, 4307, 4758, 5089
Tsp45I GTSAC 5 cut(s) 8, 2018, 3323, 3638, 4105
TspGWI ACGGA 3 cut(s) 456, 3968, 4460
TspRI CASTG 9 cut(s) 1713, 2023, 2085, 2175, 2527, 3015, 4072, 4750, 5215
Tth111I GACNNNGTC 2 cut(s) 151, 3277
VneI GTGCAC 2 cut(s) 951, 3069
VpaK11BI GGWCC 2 cut(s) 1966, 3273
XagI CCTNNNNNAGG 2 cut(s) 3490, 3533
XbaI TCTAGA 2 cut(s) 167, 4957
XceI RCATGY 6 cut(s) 178, 766, 1289, 2142, 5457, 5461
XcmI CCANNNNNNNNNTGG 1 cut(s) 1130
XhoI CTCGAG 1 cut(s) 217
XmiI GTMKAC 2 cut(s) 1642, 3445
ZrmI AGTACT 1 cut(s) 2557
Zsp2I ATGCAT 1 cut(s) 5459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.