Rh6DG322300

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005688
Physical Location & Seq
Reverse (-)
52762289 .. 52768650
6362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG322300.1

Sequence Viewer

Length: 5397 bp
ATGGCGACTGTGTCCCAAGCAGATTCTAGACGCATGTATTCTTGGTGGTGGGACAGCCACATAAGCCCAAAGAACTCGAGATGGCTGAAGGAAAATCTTACAGATATGGATGCCAAAGTCAAACACATGATCAAACTTATTGAAGAAGATGCAGATTCCTTTGCTAGAAGGGCAGAAATGTATTACAAAAAACGCCCAGAACTAATGAAATTGGTTGAAGAGTTCTACCGAGCATACCGTGCGTTAGCTGAACGATATGATCATGCAACTGGGGCACTGCGCCAGGCTCATCGAACCATGGCAGAGGCATTTCCCAACCAAGTTCCGTTTGTAGATGACTCGCCTGCAGGTTCTTCTGCTAGTGAGACTGATCCCCGAACACCTGAGATGCCAGCTCCTATACGAGCATTATTTGACCTTGATGAATTGCAAAAAGATGCTCTGGGACTCTCCTCCTCAACCCATTTCCATGCTCTCAAAAGGAATGGAGCTTTTAGTGAAGAATCTGACTCTGGAACCAGCCGAATTGGTTTGAAACAGCTGAATGATCTCTTTGGATCGGGAGAAGGAAGGGCGAAAAAGGGCCTCAACTTCCTTGATGCTGACACAAAAGAACACAGCATGCAGAACAATGGCCATGATCTTAAAAATCGAGCCTTGTTGGGGGATGATCGAGTGGGCAAAGCAGAGACAGAAATATCAAACTTAAAGAAAACTCTTGCTAAACTAGAAGCTGAAAAGGAAGCTGGCTTACTTCAGTACCAAGAGTGTTTAGAGAGAGTATCTAATTTGGAGTCTGAAGTCTCTCGTGCACAAGAGGATTCCAGGGGACTTAATGAACGAGCCAGTAAAGCTGAAGCTGAAGTTCAAACTACAAAGGAAGCACTTAACAAATTAGAGGCTGAAAGGAAAGCTAGTCTTCTTCAGTATCAGGAGTGCTTGGACAAAATCTCTAATCTGGAAAGCATCATCTCTTGTGCCCAAAAGGATGCTGGAGAGCTAAATGATCGAGCTAGTAGAGCTGAATTTGCATCTGAATCTCTACAGAAAGATCTTGAAAGGGTGGCATCTGAGAAAGAAGCTGCACTTGCACAATACGAACAGTGTCTGGAGAATATATCAAATCTGGAGGACAAACTACTGCATGTTGAGGAGGATGCCAAGAAGATTAACGAGCGTGCTGTTAAAGCTGAACTCGAAGTTGAATCCTTGAAGCAAGCAATTGCCAATTTAACTGAAGAGAAGGAAGCTGCTGCTCTCCAGTACAAGCAATGCCTGGAAACGATATCTAATCTTGAGCATAAGATCTCTCATGTGGAGGAGGAGGCACTACGGCTACACTCTCAGATAGACGACGGGATTGTGAAGTTCAAGGATTCGGAAGAAAAGTGTCTTCTGCTGGTAAACTCAAATCAAAATCTGCAGTTGGAGTTGGAGTCTGTGGTGAAGCAAATGCAATCTCAAGGTGAAGAGCTTACAGAGAAGCAGAAGGAGTTGGGTAGACTTTGGGCTTGCATACAAGAAGAGCGGTTGCGATTTATGGAGGCTGAAACTGCTTTCCAGACACTGCAGCATTTGCATTCCCAGTCCCAGGAGGAACTTAGATCTCTTGTAGCTGAGCTCCAGAATAGAGATTTGATTCTGAAGGACATGGAAGCACGAAGTCAGAGATTGGATGATGAAGTCCAGAAGGTGAAGGAGGAAAACAAGAGCCTAAGTGAGATCAATTTGTCTTCATCTGTTTCAATAAAAGATCTGCAAGGTGAGATCTTAATCTTGAGGGAGACGATTAAGAAACTTGAAGAGGAAGTTGAACTTCGAGTGGACCAAAGAAATGCTCTTCAGCAAGAGATATACTGTCTGAAAGAGGAACTCAGTGACCTGAACAAGAAACACCAAGCTATGCTAGAGCAGGTGGAGTCAGTTGGCTTGGATCCAGTGTGCATTGGGTCATCTGTGAAGGAGTTGCAAGATGAGAACTTACAGCTCAAACAAATATGTGAGGCTGATAAAAGTGAGAAAGTGGCACTGTTGGAAAAGTTGGAAATCATGCAGAAACTTCGGGAGAAAAATGTTCTTTTGGATAATTCCCTTTCTGATTTGAATGTTGAGTTAGAAGGGGTTAGAGGGAAGGTAAAGGAGTTGGAACAATCATGTCAGTCTCTTCTGGCAGAAAAAGGTACTCTTCTTGCTGAAAATGGCACTCTGATTTCCCAGTTGCAGTTTATGACTGAGAATTTAGAAAAATCATCAGAGAAGAACAAATTTTTGGAGAATTCTCTCTTTGATGCGAATGCTGAACTGGAAGGCTTGAGTGTAAAATCAAAGAGCTTAGAAGAATCATGCCTACTGCTTGGTAATGATAAGACTGGTCTGATCACTGAGAGAGAGAGTTTAATTTTGGAGTTGGGCAGTACTCGGCAAAGACTGGAAGATCTGGAAAAAGGATATGCAGAGATAGAAGAGAAACTCTCTGTTCTGGAGAAGGAAAGAGATTCTGCACTTTGTAAAGTGGAAGAGCTAAATGTATGCTTAGATTCTGAGAAGCAAAATCATGCCAGTTCTGTTGAGTTAAGGGAAACCCAGTTGGCTGAAATGGAACTGAAGATCTCTCATCTCCAAGCAGAAGGGATTTGCAGGAAGAAAGAATTTGAAGAGGAACAAGACAAATCTGTTACTGCACAGATAGAAATCTTTGTCCTGCAGAAATGTGTAGAAGATCTGGAAGAGAAGAACTTATCCCTCATGATTGAGCATCAAAAGCTTTTGGGGGCATCCGCAATGTCAGAGAAGCTGATTTCTGTCCTAGAGCATGGCAAACTTGAACAACAGAAGGAAATTAAGTCATTGTTTGTGCAAATTAAAGCACTGAGAATGGGGCTGTATCAGGTGTTGAAGAATGTTGACATTGATGCAAACCTTGGGTGTGCAGAAAAGGATGAGCAAGACCAAGCACTCCTTAATCATATACTTGTCAAACTTCAAGACAAACAAAATTCCTTCTCTGAAAGTTGTGATGAAAACCAGCAGTTGCTTATTGAGAACTCTGTTCTCATAGCAATGCTTGCCCAAATGAAACTAGAGGCGGACTGTTTTATGAGGGAAAGAAACACTCTTGACCATGAGTTCAGGACCCAGTCTGAGAAGTTCTTGGTGTTGCAGAGTGGCGCCCAAAGACTTCGTGACATGAATGCAGAATTGAATTTGAAGGTAGTGGAGGGAGAACACAGAGAGGGAGTTTTGAGGACTGAAATAGATAATCTGCACGAGCAGTTGTTGGACTTGCAAAGCATTTACAAGAGTCTACAAAAAGAAAATTCCCAGATGGTTGAAGATAAAGGATCCTTGAAAAGGACGATCTTGAATTTGGAGGAAGAGGCGCGTAACCTAGAAGAGGATAAATGCGTCATGTTTGCTGAAACAATATATTATAGTAACCTCTCCCTTGTTTTCGATGATATCATTTCCCAAAAGCAACTGGAACTGGAAGAGCTCAGTCACAATTATGATGAACTTCATCTTGGCAATAATGACCTGAAAGAGAAGGTGAGAGTATTGGAGGGGCAGTTAGAAGTTATACAGATGGAAAACTTACATCTTAAGGAGTCATTAAATAAGTCGGAGGATGAGCTGAAATTGGTTAAATCTGTGAATGATCAATTGAATGGTGATATTGCAAATGCAAAGGATGGTTTGTCTCAAAAGGAAAATGAGCTTTTGGAAGCCGGGCAGATCATAAATGAACTACAGAATGAGAAAAAAGAGCTGTATATACTGGTGGAGGATTTGACTAGTAAAAGTGATGATGCGAAGGTGGTGCTGGAAGATCAAGAGAAGCAAATTTTCAAACTACATGACGACAATGACCGTCATAGTAAAGAGATTGGATGCCTCCGTGAAGTGAATCAGAAGTTGGAGGTGGAACTATCGAAATTGCATGAAGAAGCTGAAAAAGCCAAAATTGAGGAGGAAAGGTTGATCAGTGAACTAAAAAGGGGAAGAGAAGAGATAGAAATGTGGGTGACTCACGCAGCCACCCTCTTTGGAGAACTGCAGATCTCCTCCATTCGTGAAACATTGTTTGAAGGAAAGATCCGCGAGCTCATTGAAGCCTGTCAGATCCTTGAAGACAAAAGCGTTTCCAAAGGTCTGGAGAGTGAACAGATGAAAGAAAGAGTCGGCACATTGGAACATGAAAATGGAGAGCTACAAGCTCAGTTGGCTGCCTATGTTCCAGCTGTCATCTCTCTGAAGGAGTGTACAACAGCCCTTGAGAATCATTCACTTATTAACACCAGATGTCATAAACTTGACACTGAAGCCTCAGAGGATGCCCTTCTGCAAGCTGAGAGCTCTCAAACGGATGGACATCAAATTGACACAGTGCCAGATGGCATTTCAGGACTGCAGGATTTACAAAGAAGAATCAAAGCTATTGAAAAGGCTATGGTAGAAAAGGAAAGCCATCTTACTGCCAATCAGGAAGGTAAACAATTTGGGGACAGGTTCGGTAGTGAGAAGCCTGAAATCTCTGAAGTGGGGAACGAAGTTCTGACGAAGGACATCATTCTCGATCAGATATCAGAGTGTTCATCCTATGGGGTAAGCAGGAGAGAAACCACAGAGCCTGATGCTCAGATGATGGAGTTATGGGAGACCACTGACCGGGATGGCAGCATTGATTTGATGGTTGGCAAGGCCCAGAAGGCAACTACTGTACCGACTGACCACAGTCAGACTGAAGCTATAAAGAAACACAAGAATAAATATCCCTCTTCAGAATCATTGGTTGAGAAGGAATATAGCGTTGACAAATTAGAGATCTCTAAGAGATTCTCCGAGCCCCGTCAAGAAGGCAACAAGAGAAGGATTCTAGAAAGACTTGATTCTGATGTTCAAAAGTTGACAAACCTTCAAATTACTGTGGAAGACCTGAAGAAGAAGGTGGACATTACTGAGAAGACCAAAAAGGGCAAAGGTATTGAATTTGGTACTGTAAAAGAGCAGCTGGATGAAGCCGAAGAAGCCATCACTAGATTGTTTGATGCCAATAACAAATTGATGAAGAGTGTCGAAGATGAATTTGTGTCTCCTGATGGAGCTTCTGAGATAGTCCCAGATCACAGTGGGACTGTCAGCAGAAGGAGACTGTCCGAACAAGCAAAAAGAGGGTCAGAGAAAATCGGTCGATTGCAGTTGGAGGTACAGAAACTGCAATTTCTTCTACTGAAGCTGGATGGTGAAAAGGAAAGCAAAGGGTCAACCAGGATCAAGGAGAGGAAAACAAGAGTTCTTCTGCGAGACTATCTCTATTCTGGGACTAGAATGAGCAACACAACCCCAAAGCGTAAGAAAGCTCCTTTTTGTGCATGCATGCCCCCAACTAAGGGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIP1

Protein Analysis

1798

Amino Acids

204.66

Weight (kDa)

4.97

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 14 - 87 1.7e-37 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1903
Acc36I ACCTGC 2 cut(s) 338, 1903
AccB1I GGYRCC 1 cut(s) 3167
AccBSI CCGCTC 1 cut(s) 1528
AccI GTMKAC 2 cut(s) 1501, 3304
AccII CGCG 2 cut(s) 3382, 4098
AciI CCGC 4 cut(s) 1528, 2778, 3086, 4096
AclWI GGATC 9 cut(s) 365, 565, 1928, 1941, 3336, 3349, 4087, 4114, 5276
AcoI YGGCCR 1 cut(s) 634
AcyI GRCGYC 1 cut(s) 3168
AfaI GTAC 8 cut(s) 761, 1265, 2182, 2416, 4261, 4719, 4993, 5205
AfiI CCNNNNNNNGG 7 cut(s) 663, 1591, 3351, 3394, 4665, 5386, 5387
AflII CTTAAG 1 cut(s) 3599
AhlI ACTAGT 1 cut(s) 3791
AjnI CCWGG 5 cut(s) 282, 824, 1275, 1590, 5264
AjuI GAANNNNNNNTTGG 6 cut(s) 2252, 2284, 3504, 3536, 3896, 3928
Alw21I GWGCWC 5 cut(s) 814, 1623, 3495, 4104, 4355
Alw44I GTGCAC 1 cut(s) 810
AlwI GGATC 9 cut(s) 365, 565, 1928, 1941, 3336, 3349, 4087, 4114, 5276
AlwNI CAGNNNCTG 4 cut(s) 1108, 1567, 4628, 5212
Ama87I CYCGRG 1 cut(s) 76
AoxI GGCC 3 cut(s) 583, 634, 4698
ApaLI GTGCAC 1 cut(s) 810
ApeKI GCWGC 8 cut(s) 1082, 1250, 1253, 1570, 4031, 4223, 4674, 5005
ArsI GACNNNNNNTTYG 2 cut(s) 5156, 5188
AspLEI GCGC 3 cut(s) 282, 3170, 3382
AspS9I GGNCC 4 cut(s) 583, 1825, 3132, 4699
AsuC2I CCSGG 2 cut(s) 3727, 4667
AsuHPI GGTGA 8 cut(s) 1456, 1478, 1705, 1775, 3559, 3680, 4033, 5252
AvaI CYCGRG 1 cut(s) 76
AvaII GGWCC 2 cut(s) 1825, 3132
BaeGI GKGCMC 3 cut(s) 277, 814, 982
BaeI ACNNNNGTAYC 4 cut(s) 743, 776, 4701, 4734
BalI TGGCCA 1 cut(s) 636
BamHI GGATCC 2 cut(s) 1933, 3341
BanI GGYRCC 1 cut(s) 3167
BanII GRGCYC 5 cut(s) 1623, 3495, 4104, 4355, 4845
BarI GAAGNNNNNNTAC 2 cut(s) 735, 767
BauI CACGAG 2 cut(s) 807, 3266
BbsI GAAGAC 6 cut(s) 911, 1385, 1725, 4134, 4935, 4967
Bbv12I GWGCWC 5 cut(s) 814, 1623, 3495, 4104, 4355
BbvI GCAGC 8 cut(s) 1069, 1237, 1240, 1582, 4043, 4210, 4686, 5017
BceAI ACGGC 1 cut(s) 1349
BcgI CGANNNNNNTGC 4 cut(s) 2042, 2076, 3799, 3833
BciT130I CCWGG 5 cut(s) 284, 826, 1277, 1592, 5266
BclI TGATCA 5 cut(s) 129, 259, 2376, 3655, 3978
BcnI CCSGG 2 cut(s) 3727, 4667
BcuI ACTAGT 1 cut(s) 3791
BfmI CTRYAG 8 cut(s) 345, 1043, 1421, 1568, 2702, 3746, 4052, 4406
BfoI RGCGCY 1 cut(s) 3171
BfrI CTTAAG 1 cut(s) 3599
BfuAI ACCTGC 2 cut(s) 338, 1903
BglI GCCNNNNNGGC 1 cut(s) 4706
BisI GCNGC 8 cut(s) 1083, 1251, 1254, 1571, 4032, 4224, 4675, 5006
BlpI GCTNAGC 1 cut(s) 1617
BlsI GCNGC 8 cut(s) 1084, 1252, 1255, 1572, 4033, 4225, 4676, 5007
BmcAI AGTACT 1 cut(s) 2416
Bme1390I CCNGG 7 cut(s) 284, 826, 1277, 1592, 3727, 4667, 5266
Bme18I GGWCC 2 cut(s) 1825, 3132
BmeT110I CYCGRG 1 cut(s) 76
BmgT120I GGNCC 4 cut(s) 583, 1825, 3132, 4699
BmiI GGNNCC 5 cut(s) 517, 1935, 3134, 3169, 3343
BmrFI CCNGG 7 cut(s) 284, 826, 1277, 1592, 3727, 4667, 5266
BmrI ACTGGG 5 cut(s) 279, 1579, 2209, 2578, 3130
BmuI ACTGGG 5 cut(s) 279, 1579, 2209, 2578, 3130
BoxI GACNNNNGTC 1 cut(s) 4731
BpiI GAAGAC 6 cut(s) 911, 1385, 1725, 4134, 4935, 4967
BplI GAGNNNNNCTC 4 cut(s) 2456, 2488, 5292, 5324
BpmI CTGGAG 7 cut(s) 1014, 1130, 1148, 1244, 1607, 2501, 4172
Bpu1102I GCTNAGC 1 cut(s) 1617
BpuEI CTTGAG 5 cut(s) 1316, 1446, 1798, 2332, 4292
BpuMI CCSGG 2 cut(s) 3727, 4667
BsaBI GATNNNNATC 3 cut(s) 1772, 2690, 4368
BsaHI GRCGYC 1 cut(s) 3168
BsaI GGTCTC 1 cut(s) 4649
BsaJI CCNNGG 4 cut(s) 297, 825, 1590, 2920
BsaXI ACNNNNNCTCC 6 cut(s) 1421, 1451, 3551, 3581, 3959, 3989
Bsc4I CCNNNNNNNGG 7 cut(s) 663, 1591, 3351, 3394, 4665, 5386, 5387
Bse3DI GCAATG 3 cut(s) 1277, 2787, 3066
Bse8I GATNNNNATC 3 cut(s) 1772, 2690, 4368
BseBI CCWGG 5 cut(s) 284, 826, 1277, 1592, 5266
BseDI CCNNGG 4 cut(s) 297, 825, 1590, 2920
BseJI GATNNNNATC 3 cut(s) 1772, 2690, 4368
BseLI CCNNNNNNNGG 7 cut(s) 663, 1591, 3351, 3394, 4665, 5386, 5387
BseMI GCAATG 3 cut(s) 1277, 2787, 3066
BseRI GAGGAG 7 cut(s) 442, 445, 1166, 1334, 1337, 3980, 4051
BseSI GKGCMC 3 cut(s) 277, 814, 982
BseXI GCAGC 8 cut(s) 1069, 1237, 1240, 1582, 4043, 4210, 4686, 5017
BsgI GTGCAG 5 cut(s) 1068, 2484, 2664, 2949, 3248
Bsh1236I CGCG 2 cut(s) 3382, 4098
Bsh1285I CGRYCG 1 cut(s) 5188
BshFI GGCC 3 cut(s) 585, 636, 4700
BshNI GGYRCC 1 cut(s) 3167
BsiEI CGRYCG 1 cut(s) 5188
BsiHKAI GWGCWC 5 cut(s) 814, 1623, 3495, 4104, 4355
BsiHKCI CYCGRG 1 cut(s) 76
BsiSI CCGG 2 cut(s) 3726, 4666
BslFI GGGAC 8 cut(s) 65, 459, 843, 1573, 4514, 5099, 5143, 5332
BslI CCNNNNNNNGG 7 cut(s) 663, 1591, 3351, 3394, 4665, 5386, 5387
BsmBI CGTCTC 1 cut(s) 1778
BsmFI GGGAC 8 cut(s) 65, 459, 843, 1573, 4514, 5099, 5143, 5332
BsmI GAATGC 3 cut(s) 1579, 2299, 3196
BsnI GGCC 3 cut(s) 585, 636, 4700
Bso31I GGTCTC 1 cut(s) 4649
BsoBI CYCGRG 1 cut(s) 76
Bsp1286I GDGCHC 8 cut(s) 277, 814, 982, 1623, 3495, 4104, 4355, 4845
Bsp1407I TGTACA 1 cut(s) 4259
Bsp1720I GCTNAGC 1 cut(s) 1617
Bsp19I CCATGG 1 cut(s) 297
BspACI CCGC 4 cut(s) 1528, 2778, 3086, 4096
BspANI GGCC 3 cut(s) 585, 636, 4700
BspFNI CGCG 2 cut(s) 3382, 4098
BspHI TCATGA 1 cut(s) 2745
BspLI GGNNCC 5 cut(s) 517, 1935, 3134, 3169, 3343
BspMAI CTGCAG 6 cut(s) 349, 1425, 1572, 2706, 4056, 4410
BspMI ACCTGC 2 cut(s) 338, 1903
BspPI GGATC 9 cut(s) 365, 565, 1928, 1941, 3336, 3349, 4087, 4114, 5276
BspQI GCTCTTC 5 cut(s) 1464, 1518, 1845, 2511, 3483
BspT107I GGYRCC 1 cut(s) 3167
BspTI CTTAAG 1 cut(s) 3599
BspTNI GGTCTC 1 cut(s) 4649
BsrBI CCGCTC 1 cut(s) 1528
BsrDI GCAATG 3 cut(s) 1277, 2787, 3066
BsrGI TGTACA 1 cut(s) 4259
BssECI CCNNGG 4 cut(s) 297, 825, 1590, 2920
BssNI GRCGYC 1 cut(s) 3168
BssSI CACGAG 2 cut(s) 807, 3266
BssT1I CCWWGG 2 cut(s) 297, 2920
Bst2BI CACGAG 2 cut(s) 807, 3266
Bst2UI CCWGG 5 cut(s) 284, 826, 1277, 1592, 5266
BstACI GRCGYC 1 cut(s) 3168
BstAFI CTTAAG 1 cut(s) 3599
BstAPI GCANNNNNTGC 3 cut(s) 239, 1576, 3065
BstAUI TGTACA 1 cut(s) 4259
BstDSI CCRYGG 1 cut(s) 297
BstENI CCTNNNNNAGG 2 cut(s) 3349, 3392
BstFNI CGCG 2 cut(s) 3382, 4098
BstH2I RGCGCY 1 cut(s) 3171
BstHHI GCGC 3 cut(s) 282, 3170, 3382
BstMCI CGRYCG 1 cut(s) 5188
BstNI CCWGG 5 cut(s) 284, 826, 1277, 1592, 5266
BstNSI RCATGY 5 cut(s) 37, 625, 1148, 5373, 5377
BstPAI GACNNNNGTC 1 cut(s) 4731
BstSCI CCNGG 7 cut(s) 282, 824, 1275, 1590, 3725, 4665, 5264
BstSFI CTRYAG 8 cut(s) 345, 1043, 1421, 1568, 2702, 3746, 4052, 4406
BstSLI GKGCMC 3 cut(s) 277, 814, 982
BstUI CGCG 2 cut(s) 3382, 4098
BstV1I GCAGC 8 cut(s) 1069, 1237, 1240, 1582, 4043, 4210, 4686, 5017
BstV2I GAAGAC 6 cut(s) 911, 1385, 1725, 4134, 4935, 4967
BstXI CCANNNNNNTGG 1 cut(s) 4150
BsuRI GGCC 3 cut(s) 585, 636, 4700
BtgI CCRYGG 1 cut(s) 297
BtsI GCAGTG 2 cut(s) 275, 1565
BveI ACCTGC 2 cut(s) 338, 1903
CaiI CAGNNNCTG 4 cut(s) 1108, 1567, 4628, 5212
CciI TCATGA 1 cut(s) 2745
CfoI GCGC 3 cut(s) 282, 3170, 3382
Cfr13I GGNCC 4 cut(s) 583, 1825, 3132, 4699
CseI GACGC 2 cut(s) 39, 3394
Csp6I GTAC 8 cut(s) 760, 1264, 2181, 2415, 4260, 4718, 4992, 5204
CspCI CAANNNNNGTGG 2 cut(s) 4024, 4059
CviQI GTAC 8 cut(s) 760, 1264, 2181, 2415, 4260, 4718, 4992, 5204
DinI GGCGCC 1 cut(s) 3169
EaeI YGGCCR 1 cut(s) 634
EciI GGCGGA 1 cut(s) 3101
Ecl136II GAGCTC 4 cut(s) 1621, 3493, 4102, 4353
Eco130I CCWWGG 2 cut(s) 297, 2920
Eco24I GRGCYC 5 cut(s) 1623, 3495, 4104, 4355, 4845
Eco31I GGTCTC 1 cut(s) 4649
Eco32I GATATC 3 cut(s) 1287, 3460, 4581
Eco47I GGWCC 2 cut(s) 1825, 3132
Eco53kI GAGCTC 4 cut(s) 1621, 3493, 4102, 4353
Eco88I CYCGRG 1 cut(s) 76
EcoICRI GAGCTC 4 cut(s) 1621, 3493, 4102, 4353
EcoNI CCTNNNNNAGG 2 cut(s) 3349, 3392
EcoO109I RGGNCCY 2 cut(s) 583, 3132
EcoRI GAATTC 1 cut(s) 2275
EcoRII CCWGG 5 cut(s) 282, 824, 1275, 1590, 5264
EcoRV GATATC 3 cut(s) 1287, 3460, 4581
EcoT14I CCWWGG 2 cut(s) 297, 2920
EcoT22I ATGCAT 1 cut(s) 5375
EcoT38I GRGCYC 5 cut(s) 1623, 3495, 4104, 4355, 4845
EgeI GGCGCC 1 cut(s) 3169
EheI GGCGCC 1 cut(s) 3169
ErhI CCWWGG 2 cut(s) 297, 2920
Esp3I CGTCTC 1 cut(s) 1778
FaqI GGGAC 8 cut(s) 65, 459, 843, 1573, 4514, 5099, 5143, 5332
FbaI TGATCA 5 cut(s) 129, 259, 2376, 3655, 3978
FblI GTMKAC 2 cut(s) 1501, 3304
Fnu4HI GCNGC 8 cut(s) 1083, 1251, 1254, 1571, 4032, 4224, 4675, 5006
FriOI GRGCYC 5 cut(s) 1623, 3495, 4104, 4355, 4845
Fsp4HI GCNGC 8 cut(s) 1083, 1251, 1254, 1571, 4032, 4224, 4675, 5006
GlaI GCGC 3 cut(s) 281, 3169, 3381
GluI GCNGC 8 cut(s) 1083, 1251, 1254, 1571, 4032, 4224, 4675, 5006
GsuI CTGGAG 7 cut(s) 1014, 1130, 1148, 1244, 1607, 2501, 4172
HaeII RGCGCY 1 cut(s) 3171
HaeIII GGCC 3 cut(s) 585, 636, 4700
HapII CCGG 2 cut(s) 3726, 4666
HgaI GACGC 2 cut(s) 39, 3394
HhaI GCGC 3 cut(s) 282, 3170, 3382
Hin1I GRCGYC 1 cut(s) 3168
Hin6I GCGC 3 cut(s) 280, 3168, 3380
HinP1I GCGC 3 cut(s) 280, 3168, 3380
HincII GTYRAC 4 cut(s) 2905, 4810, 4905, 5262
HindII GTYRAC 4 cut(s) 2905, 4810, 4905, 5262
HindIII AAGCTT 1 cut(s) 2762
HpaII CCGG 2 cut(s) 3726, 4666
HphI GGTGA 8 cut(s) 1456, 1478, 1705, 1775, 3559, 3680, 4033, 5252
Hpy99I CGWCG 1 cut(s) 1358
Hsp92I GRCGYC 1 cut(s) 3168
HspAI GCGC 3 cut(s) 280, 3168, 3380
KasI GGCGCC 1 cut(s) 3167
Ksp22I TGATCA 5 cut(s) 129, 259, 2376, 3655, 3978
LguI GCTCTTC 5 cut(s) 1464, 1518, 1845, 2511, 3483
LmnI GCTCC 5 cut(s) 400, 488, 1626, 5099, 5362
Lsp1109I GCAGC 8 cut(s) 1069, 1237, 1240, 1582, 4043, 4210, 4686, 5017
MaeIII GTNAC 7 cut(s) 1877, 2674, 3182, 3383, 3434, 3497, 4021
MbiI CCGCTC 1 cut(s) 1528
MfeI CAATTG 2 cut(s) 1221, 3659
MhlI GDGCHC 8 cut(s) 277, 814, 982, 1623, 3495, 4104, 4355, 4845
MlsI TGGCCA 1 cut(s) 636
MluNI TGGCCA 1 cut(s) 636
Mly113I GGCGCC 1 cut(s) 3168
MmeI TCCRAC 9 cut(s) 1407, 1413, 2013, 2022, 2124, 3258, 3600, 3894, 5178
Mox20I TGGCCA 1 cut(s) 636
Mph1103I ATGCAT 1 cut(s) 5375
MscI TGGCCA 1 cut(s) 636
MslI CAYNNNNRTG 4 cut(s) 468, 3059, 3504, 4197
Msp20I TGGCCA 1 cut(s) 636
MspA1I CMGCKG 3 cut(s) 541, 4238, 5008
MspCI CTTAAG 1 cut(s) 3599
MspI CCGG 2 cut(s) 3726, 4666
MspR9I CCNGG 7 cut(s) 284, 826, 1277, 1592, 3727, 4667, 5266
MunI CAATTG 2 cut(s) 1221, 3659
Mva1269I GAATGC 3 cut(s) 1579, 2299, 3196
MvaI CCWGG 5 cut(s) 284, 826, 1277, 1592, 5266
MvnI CGCG 2 cut(s) 3382, 4098
NarI GGCGCC 1 cut(s) 3168
NciI CCSGG 2 cut(s) 3727, 4667
NcoI CCATGG 1 cut(s) 297
NlaIV GGNNCC 5 cut(s) 517, 1935, 3134, 3169, 3343
NmeAIII GCCGAG 1 cut(s) 2398
NmuCI GTSAC 4 cut(s) 1877, 3182, 3497, 4021
NsiI ATGCAT 1 cut(s) 5375
NspI RCATGY 5 cut(s) 37, 625, 1148, 5373, 5377
PaeI GCATGC 3 cut(s) 625, 5373, 5377
PaeR7I CTCGAG 1 cut(s) 76
PagI TCATGA 1 cut(s) 2745
PaqCI CACCTGC 1 cut(s) 1903
PciSI GCTCTTC 5 cut(s) 1464, 1518, 1845, 2511, 3483
PctI GAATGC 3 cut(s) 1579, 2299, 3196
PflFI GACNNNGTC 2 cut(s) 10, 3136
PkrI GCNGC 8 cut(s) 1084, 1252, 1255, 1572, 4033, 4225, 4676, 5007
PluTI GGCGCC 1 cut(s) 3171
PpuMI RGGWCCY 1 cut(s) 3132
PshAI GACNNNNGTC 1 cut(s) 4731
Psp124BI GAGCTC 4 cut(s) 1623, 3495, 4104, 4355
Psp5II RGGWCCY 1 cut(s) 3132
Psp6I CCWGG 5 cut(s) 282, 824, 1275, 1590, 5264
PspGI CCWGG 5 cut(s) 282, 824, 1275, 1590, 5264
PspN4I GGNNCC 5 cut(s) 517, 1935, 3134, 3169, 3343
PspPI GGNCC 4 cut(s) 583, 1825, 3132, 4699
PspPPI RGGWCCY 1 cut(s) 3132
PstI CTGCAG 6 cut(s) 349, 1425, 1572, 2706, 4056, 4410
PstNI CAGNNNCTG 4 cut(s) 1108, 1567, 4628, 5212
PsyI GACNNNGTC 2 cut(s) 10, 3136
PvuII CAGCTG 3 cut(s) 541, 4238, 5008
RsaI GTAC 8 cut(s) 761, 1265, 2182, 2416, 4261, 4719, 4993, 5205
RsaNI GTAC 8 cut(s) 760, 1264, 2181, 2415, 4260, 4718, 4992, 5204
RseI CAYNNNNRTG 4 cut(s) 468, 3059, 3504, 4197
SacI GAGCTC 4 cut(s) 1623, 3495, 4104, 4355
SapI GCTCTTC 5 cut(s) 1464, 1518, 1845, 2511, 3483
SatI GCNGC 8 cut(s) 1083, 1251, 1254, 1571, 4032, 4224, 4675, 5006
Sau96I GGNCC 4 cut(s) 583, 1825, 3132, 4699
SbfI CCTGCAGG 1 cut(s) 349
ScaI AGTACT 1 cut(s) 2416
ScrFI CCNGG 7 cut(s) 284, 826, 1277, 1592, 3727, 4667, 5266
SdaI CCTGCAGG 1 cut(s) 349
SduI GDGCHC 8 cut(s) 277, 814, 982, 1623, 3495, 4104, 4355, 4845
SfcI CTRYAG 8 cut(s) 345, 1043, 1421, 1568, 2702, 3746, 4052, 4406
SfoI GGCGCC 1 cut(s) 3169
Sfr274I CTCGAG 1 cut(s) 76
SinI GGWCC 2 cut(s) 1825, 3132
SlaI CTCGAG 1 cut(s) 76
SmiMI CAYNNNNRTG 4 cut(s) 468, 3059, 3504, 4197
SmlI CTYRAG 7 cut(s) 76, 1295, 1461, 1777, 2311, 3599, 4271
SmoI CTYRAG 7 cut(s) 76, 1295, 1461, 1777, 2311, 3599, 4271
SpeI ACTAGT 1 cut(s) 3791
SphI GCATGC 3 cut(s) 625, 5373, 5377
Sse8387I CCTGCAGG 1 cut(s) 349
SsiI CCGC 4 cut(s) 1528, 2778, 3086, 4096
SspDI GGCGCC 1 cut(s) 3167
SstI GAGCTC 4 cut(s) 1623, 3495, 4104, 4355
StyD4I CCNGG 7 cut(s) 282, 824, 1275, 1590, 3725, 4665, 5264
StyI CCWWGG 2 cut(s) 297, 2920
TaqII GACCGA 1 cut(s) 5174
TatI WGTACW 3 cut(s) 1263, 2414, 4259
TseFI GTSAC 4 cut(s) 1877, 3182, 3497, 4021
TseI GCWGC 8 cut(s) 1082, 1250, 1253, 1570, 4031, 4223, 4674, 5005
Tsp45I GTSAC 4 cut(s) 1877, 3182, 3497, 4021
TspGWI ACGGA 3 cut(s) 315, 3884, 4376
Tth111I GACNNNGTC 2 cut(s) 10, 3136
Vha464I CTTAAG 1 cut(s) 3599
VneI GTGCAC 1 cut(s) 810
VpaK11BI GGWCC 2 cut(s) 1825, 3132
XagI CCTNNNNNAGG 2 cut(s) 3349, 3392
XbaI TCTAGA 2 cut(s) 26, 4873
XceI RCATGY 5 cut(s) 37, 625, 1148, 5373, 5377
XcmI CCANNNNNNNNNTGG 1 cut(s) 989
XhoI CTCGAG 1 cut(s) 76
XmiI GTMKAC 2 cut(s) 1501, 3304
ZrmI AGTACT 1 cut(s) 2416
Zsp2I ATGCAT 1 cut(s) 5375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.