FvH4_2g23771
MADS Family

MADS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011525
Physical Location & Seq
Forward (+)
19443039 .. 19444118
1080 bp
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UTR
Exon/CDS
Intron
FvH4_2g23771.t1

Sequence Viewer

Length: 1080 bp
ATGGGGAAGAAATCACAAAACACCGAATCCGGAGGAAAAGGCAAAGAACTCAAAACTCTGAAATCTCTGGAAAAACCATTCAAGAAGAGGAAGTCCACTCTGAAGAAGAGTGCCAGCGAACTTGAGATTCTCTGCCGTGCGCAGGTTTGCTGCGTTATGGAGGAGCCAACTGGGGATGTTGTGGTCTGGCCGGAAAACTACGCCGACGCCGTATCCATCATCACCAAGTACAAACAATCCAAAAAGAAAGCAAAGCTCAACCGATCCACGACTAATTTGGAGCAGCTTGAAGAAGAAAACAAGGGTTTGGCTATGGGGGATGAGGCCATTGATCAATCCTTGAATGCTTTGGAGAACCTTGAGGGGTTTGATATTGATCAATTTCTGAATAACACTATGCTCTTTGAAGATGAGAACAAGGGTTTTGATAATATTGATCAGTTTCTCATGAGTAACATTGTGGAAAATGTTGTACCAAACGAGGATACGGGTGGTACCTTTGAAATAGCTGAACCTCTAAATTATGAAGAACACAAAGATTTTGATCCGAAGGGTTTTAGGTTTGGCGATGAGGGATTAGATAGCTTGTCCGGAGAATCATGGAAGCATATTGCAGACTTCTCGGGATCAAATATTGACACAGTAGATCAAGGAATCACGTCATCAGTTGAGGGTGATGATCATGATCAACAAAACTTTGAATACCATGTTCTGAATAATAGGTCGAATTCTTTGGAGATTGAGGATAATAATGTTCTTAGCGATCATGATATTGATCAAGTTTTGAATGGCTTTTTGGGTACAGTGGGATTTCCGGAGTGTTCAAGGAGCAATGCTCACATTTCGACCGCACCAGATTTTGAGCATCATCAGATTGATAATGTACGTGGAATGTGGCTGTCGGACACATTACGGCCCTCGCCACAATACTCGGGGTTGGAGGGAGCATTCTACTTGCCTAACCACTCGACGAAATTTGAAAATTACAACAGCAATATTAGTCCCTTTTTGTCAAGCCTGGACGAGCAGCCAAAGTTGATGGGTTCCAACAAATCAATAGGAGATATTGGAAGAAATTAA

Protein Analysis

360

Amino Acids

40.17

Weight (kDa)

4.64

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 27 - 63 8e-10 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 141
Acc36I ACCTGC 1 cut(s) 133
Acc65I GGTACC 1 cut(s) 494
AccB1I GGYRCC 1 cut(s) 494
AccIII TCCGGA 3 cut(s) 29, 590, 814
AciI CCGC 1 cut(s) 849
AclWI GGATC 3 cut(s) 258, 539, 634
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 2 cut(s) 727, 974
AcuI CTGAAG 1 cut(s) 122
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 5 cut(s) 230, 474, 496, 802, 885
AfiI CCNNNNNNNGG 1 cut(s) 142
AgsI TTSAA 9 cut(s) 82, 290, 343, 407, 503, 701, 787, 825, 980
AjiI CACGTC 1 cut(s) 660
AjnI CCWGG 1 cut(s) 1017
AjuI GAANNNNNNNTTGG 2 cut(s) 779, 811
AluBI AGCT 4 cut(s) 256, 286, 509, 585
AluI AGCT 4 cut(s) 256, 286, 509, 585
AlwI GGATC 3 cut(s) 258, 539, 634
Ama87I CYCGRG 2 cut(s) 622, 931
Aor13HI TCCGGA 3 cut(s) 29, 590, 814
AoxI GGCC 3 cut(s) 188, 324, 914
ApeKI GCWGC 3 cut(s) 150, 283, 1027
ApoI RAATTY 2 cut(s) 727, 974
Asp718I GGTACC 1 cut(s) 494
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 1 cut(s) 915
AsuHPI GGTGA 2 cut(s) 214, 686
AvaI CYCGRG 2 cut(s) 622, 931
BanI GGYRCC 1 cut(s) 494
BbvI GCAGC 3 cut(s) 137, 295, 1039
BccI CCATC 2 cut(s) 224, 1033
BceAI ACGGC 3 cut(s) 120, 194, 929
BcgI CGANNNNNNTGC 2 cut(s) 603, 637
BciT130I CCWGG 1 cut(s) 1019
BciVI GTATCC 2 cut(s) 223, 478
BclI TGATCA 6 cut(s) 331, 376, 436, 679, 685, 775
BfuAI ACCTGC 1 cut(s) 133
BfuI GTATCC 2 cut(s) 223, 478
BisI GCNGC 3 cut(s) 151, 284, 1028
BlsI GCNGC 3 cut(s) 152, 285, 1029
Bme1390I CCNGG 1 cut(s) 1019
BmeT110I CYCGRG 2 cut(s) 622, 931
BmgBI CACGTC 1 cut(s) 660
BmgT120I GGNCC 1 cut(s) 915
BmiI GGNNCC 3 cut(s) 165, 496, 1045
BmrFI CCNGG 1 cut(s) 1019
BmrI ACTGGG 1 cut(s) 180
BmsI GCATC 1 cut(s) 874
BmuI ACTGGG 1 cut(s) 180
BplI GAGNNNNNCTC 2 cut(s) 820, 852
BpuEI CTTGAG 2 cut(s) 143, 380
BsaAI YACGTR 1 cut(s) 887
BsaBI GATNNNNATC 4 cut(s) 375, 543, 684, 774
BsaHI GRCGYC 1 cut(s) 207
BsaWI WCCGGW 3 cut(s) 29, 590, 814
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 175
Bse3DI GCAATG 1 cut(s) 838
Bse8I GATNNNNATC 4 cut(s) 375, 543, 684, 774
BseAI TCCGGA 3 cut(s) 29, 590, 814
BseBI CCWGG 1 cut(s) 1019
BseGI GGATG 2 cut(s) 181, 325
BseJI GATNNNNATC 4 cut(s) 375, 543, 684, 774
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMI GCAATG 1 cut(s) 838
BseNI ACTGG 1 cut(s) 175
BseRI GAGGAG 1 cut(s) 176
BseXI GCAGC 3 cut(s) 137, 295, 1039
Bsh1285I CGRYCG 1 cut(s) 849
BshFI GGCC 3 cut(s) 190, 326, 916
BshNI GGYRCC 1 cut(s) 494
BsiEI CGRYCG 1 cut(s) 849
BsiHKCI CYCGRG 2 cut(s) 622, 931
BsiSI CCGG 4 cut(s) 30, 191, 591, 815
BslFI GGGAC 1 cut(s) 987
BslI CCNNNNNNNGG 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 987
BsmI GAATGC 2 cut(s) 349, 947
BsnI GGCC 3 cut(s) 190, 326, 916
BsoBI CYCGRG 2 cut(s) 622, 931
Bsp13I TCCGGA 3 cut(s) 29, 590, 814
BspACI CCGC 1 cut(s) 849
BspANI GGCC 3 cut(s) 190, 326, 916
BspEI TCCGGA 3 cut(s) 29, 590, 814
BspHI TCATGA 3 cut(s) 447, 682, 766
BspLI GGNNCC 3 cut(s) 165, 496, 1045
BspMI ACCTGC 1 cut(s) 133
BspPI GGATC 3 cut(s) 258, 539, 634
BspT107I GGYRCC 1 cut(s) 494
BsrDI GCAATG 1 cut(s) 838
BsrI ACTGG 1 cut(s) 175
BssNI GRCGYC 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 1019
Bst4CI ACNGT 2 cut(s) 643, 805
Bst6I CTCTTC 2 cut(s) 80, 101
BstACI GRCGYC 1 cut(s) 207
BstBAI YACGTR 1 cut(s) 887
BstC8I GCNNGC 1 cut(s) 115
BstDEI CTNAG 1 cut(s) 758
BstF5I GGATG 2 cut(s) 181, 325
BstHHI GCGC 1 cut(s) 142
BstMCI CGRYCG 1 cut(s) 849
BstNI CCWGG 1 cut(s) 1019
BstSCI CCNGG 1 cut(s) 1017
BstV1I GCAGC 3 cut(s) 137, 295, 1039
BsuI GTATCC 2 cut(s) 223, 478
BsuRI GGCC 3 cut(s) 190, 326, 916
BtgZI GCGATG 1 cut(s) 582
BtrI CACGTC 1 cut(s) 660
BtsCI GGATG 2 cut(s) 181, 325
BtsIMutI CAGTG 1 cut(s) 810
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 1 cut(s) 115
CciI TCATGA 3 cut(s) 447, 682, 766
CfoI GCGC 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 915
CseI GACGC 1 cut(s) 215
Csp6I GTAC 5 cut(s) 229, 473, 495, 801, 884
CviAII CATG 5 cut(s) 448, 600, 683, 707, 767
CviQI GTAC 5 cut(s) 229, 473, 495, 801, 884
DdeI CTNAG 1 cut(s) 758
EaeI YGGCCR 1 cut(s) 188
Eam1104I CTCTTC 2 cut(s) 80, 101
EarI CTCTTC 2 cut(s) 80, 101
Eco57I CTGAAG 1 cut(s) 122
Eco88I CYCGRG 2 cut(s) 622, 931
EcoRI GAATTC 1 cut(s) 727
EcoRII CCWGG 1 cut(s) 1017
FaeI CATG 5 cut(s) 451, 603, 686, 710, 770
FaqI GGGAC 1 cut(s) 987
FatI CATG 5 cut(s) 447, 599, 682, 706, 766
FbaI TGATCA 6 cut(s) 331, 376, 436, 679, 685, 775
Fnu4HI GCNGC 3 cut(s) 151, 284, 1028
FokI GGATG 2 cut(s) 188, 332
Fsp4HI GCNGC 3 cut(s) 151, 284, 1028
FspI TGCGCA 1 cut(s) 141
GlaI GCGC 1 cut(s) 141
GluI GCNGC 3 cut(s) 151, 284, 1028
HaeIII GGCC 3 cut(s) 190, 326, 916
HapII CCGG 4 cut(s) 30, 191, 591, 815
HgaI GACGC 1 cut(s) 215
HhaI GCGC 1 cut(s) 142
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 5 cut(s) 451, 603, 686, 710, 770
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HinfI GANTC 4 cut(s) 26, 127, 596, 654
HpaII CCGG 4 cut(s) 30, 191, 591, 815
HphI GGTGA 2 cut(s) 214, 686
Hpy166II GTNNAC 1 cut(s) 96
Hpy188I TCNGA 7 cut(s) 60, 102, 387, 549, 714, 873, 904
Hpy188III TCNNGA 9 cut(s) 30, 68, 82, 448, 591, 624, 683, 767, 815
Hpy8I GTNNAC 1 cut(s) 96
Hpy99I CGWCG 2 cut(s) 209, 973
HpyAV CCTTC 1 cut(s) 544
HpyCH4III ACNGT 2 cut(s) 643, 805
HpyCH4IV ACGT 2 cut(s) 659, 886
HpyCH4V TGCA 1 cut(s) 614
HpyF3I CTNAG 1 cut(s) 758
HpySE526I ACGT 2 cut(s) 659, 886
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 5 cut(s) 451, 603, 686, 710, 770
HspAI GCGC 1 cut(s) 140
Kpn2I TCCGGA 3 cut(s) 29, 590, 814
KpnI GGTACC 1 cut(s) 498
Ksp22I TGATCA 6 cut(s) 331, 376, 436, 679, 685, 775
LmnI GCTCC 4 cut(s) 163, 280, 828, 944
Lsp1109I GCAGC 3 cut(s) 137, 295, 1039
LweI GCATC 1 cut(s) 874
MaeII ACGT 2 cut(s) 659, 886
MaeIII GTNAC 1 cut(s) 452
MboII GAAGA 8 cut(s) 19, 97, 115, 118, 302, 305, 419, 539
MluCI AATT 7 cut(s) 274, 380, 520, 727, 974, 982, 1075
MmeI TCCRAC 3 cut(s) 882, 918, 1071
MroI TCCGGA 3 cut(s) 29, 590, 814
MseI TTAA 1 cut(s) 1078
MspI CCGG 4 cut(s) 30, 191, 591, 815
MspR9I CCNGG 1 cut(s) 1019
Mva1269I GAATGC 2 cut(s) 349, 947
MvaI CCWGG 1 cut(s) 1019
NlaIII CATG 5 cut(s) 451, 603, 686, 710, 770
NlaIV GGNNCC 3 cut(s) 165, 496, 1045
NsbI TGCGCA 1 cut(s) 141
PagI TCATGA 3 cut(s) 447, 682, 766
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 2 cut(s) 349, 947
PfeI GAWTC 4 cut(s) 26, 127, 596, 654
PkrI GCNGC 3 cut(s) 152, 285, 1029
Ppu21I YACGTR 1 cut(s) 887
Psp6I CCWGG 1 cut(s) 1017
PspGI CCWGG 1 cut(s) 1017
PspN4I GGNNCC 3 cut(s) 165, 496, 1045
PspPI GGNCC 1 cut(s) 915
RsaI GTAC 5 cut(s) 230, 474, 496, 802, 885
RsaNI GTAC 5 cut(s) 229, 473, 495, 801, 884
SaqAI TTAA 1 cut(s) 1078
SatI GCNGC 3 cut(s) 151, 284, 1028
Sau96I GGNCC 1 cut(s) 915
ScrFI CCNGG 1 cut(s) 1019
SfaNI GCATC 1 cut(s) 874
SmlI CTYRAG 2 cut(s) 122, 359
SmoI CTYRAG 2 cut(s) 122, 359
Sse9I AATT 7 cut(s) 274, 380, 520, 727, 974, 982, 1075
SsiI CCGC 1 cut(s) 849
SspI AATATT 3 cut(s) 433, 634, 997
StyD4I CCNGG 1 cut(s) 1017
TaaI ACNGT 2 cut(s) 643, 805
TaiI ACGT 2 cut(s) 662, 889
TaqI TCGA 3 cut(s) 725, 845, 968
TasI AATT 7 cut(s) 274, 380, 520, 727, 974, 982, 1075
TatI WGTACW 1 cut(s) 228
TfiI GAWTC 4 cut(s) 26, 127, 596, 654
Tru1I TTAA 1 cut(s) 1078
Tru9I TTAA 1 cut(s) 1078
TscAI CASTG 1 cut(s) 810
TseI GCWGC 3 cut(s) 150, 283, 1027
TspDTI ATGAA 1 cut(s) 540
TspRI CASTG 1 cut(s) 810
XapI RAATTY 2 cut(s) 727, 974
XcmI CCANNNNNNNNNTGG 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.