Rh6BG337200
MADS Family

MADS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0011525
Physical Location & Seq
Forward (+)
57023808 .. 57024622
815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG337200.1

Sequence Viewer

Length: 699 bp
ATGGTGACGAAATCCCAGACCACCAAATCCGGAGAAAGGATCAAAGAGATCAGAAGTCTGAGATCTTTGGAAAAACCAATTCAGAAGAGAAAGTCCACTCTGAAGAAGAGCGCGAGCCAGCTCGAGATTCTCTGCGGTGCGCAGGTTTGCTGCATTTTGGTGGGGCCAACTGGGAATGTTGAGGTCTGGCCGGAAAACCCAGCCGACGCCGTATCCATTATCACCAAGTACAAACAATGCAAAAAGAAAACAAAGGTTTGTAGGGAGGTCAACCGATCCAAGACCGTGGAGCAGTTTGAAGAAAATAAGGGTTTGGCTATGGGTGATGATGACATTGATCAATCTCTGGAGAACTTTGAGGAAGACAAGGGGTTTGATATTGATCAATTTCTGAATAACACTATGGAGAACTTTGAAGATCAAAACAAGTGTATCGATATGGGTGATGATATTGATCAATTTCTGAATAACACTATGGAGAACTTTGAAGATCAAAACAAGTGTATCGATATGGGTGATGATATTGATCAATTTCTCATGAATAACACAATGGAAAATGTTGAACCAAACAACGGTTTTGATACCGATCATGATTTTGATCAATTTCTGAATGATTTTTTTGGTGATACTAATGATCTTAAAGTGGGCTTTCCAGAGTGGTTCAACCAGCAATGCTCACATGCCAACACCAGGGTTTGA

Protein Analysis

232

Amino Acids

26.66

Weight (kDa)

4.5

Isoelectric Point (pI)

24.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 28 - 63 7.4e-10 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 141
Acc36I ACCTGC 1 cut(s) 133
AccII CGCG 1 cut(s) 113
AccIII TCCGGA 1 cut(s) 29
AciI CCGC 1 cut(s) 135
AclWI GGATC 2 cut(s) 47, 270
AcoI YGGCCR 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 122
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 3 cut(s) 36, 572, 690
AgsI TTSAA 5 cut(s) 299, 416, 488, 563, 664
AjnI CCWGG 1 cut(s) 689
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
AlwI GGATC 2 cut(s) 47, 270
Ama87I CYCGRG 1 cut(s) 122
Aor13HI TCCGGA 1 cut(s) 29
AoxI GGCC 2 cut(s) 164, 188
ApeKI GCWGC 1 cut(s) 150
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
AspLEI GCGC 2 cut(s) 113, 142
AspS9I GGNCC 1 cut(s) 164
AsuHPI GGTGA 6 cut(s) 16, 214, 335, 455, 527, 635
AvaI CYCGRG 1 cut(s) 122
BaeI ACNNNNGTAYC 4 cut(s) 415, 448, 487, 520
BbsI GAAGAC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 194
BciT130I CCWGG 1 cut(s) 691
BciVI GTATCC 1 cut(s) 223
BclI TGATCA 5 cut(s) 337, 382, 454, 526, 598
BfuAI ACCTGC 1 cut(s) 133
BfuI GTATCC 1 cut(s) 223
BglII AGATCT 1 cut(s) 62
BisI GCNGC 1 cut(s) 151
BlsI GCNGC 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 691
BmeT110I CYCGRG 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 1 cut(s) 165
BmrFI CCNGG 1 cut(s) 691
BmrI ACTGGG 1 cut(s) 180
BmuI ACTGGG 1 cut(s) 180
BpiI GAAGAC 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 368
Bsa29I ATCGAT 2 cut(s) 435, 507
BsaBI GATNNNNATC 5 cut(s) 381, 453, 525, 585, 597
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 285, 690
BsaWI WCCGGW 1 cut(s) 29
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 572, 690
Bse1I ACTGG 1 cut(s) 175
Bse3DI GCAATG 1 cut(s) 677
Bse8I GATNNNNATC 5 cut(s) 381, 453, 525, 585, 597
BseAI TCCGGA 1 cut(s) 29
BseBI CCWGG 1 cut(s) 691
BseCI ATCGAT 2 cut(s) 435, 507
BseDI CCNNGG 2 cut(s) 285, 690
BseJI GATNNNNATC 5 cut(s) 381, 453, 525, 585, 597
BseLI CCNNNNNNNGG 3 cut(s) 36, 572, 690
BseMI GCAATG 1 cut(s) 677
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 1 cut(s) 137
BseYI CCCAGC 1 cut(s) 199
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 2 cut(s) 166, 190
BshVI ATCGAT 2 cut(s) 435, 507
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 2 cut(s) 30, 191
BslI CCNNNNNNNGG 3 cut(s) 36, 572, 690
BsnI GGCC 2 cut(s) 166, 190
BsoBI CYCGRG 1 cut(s) 122
Bsp13I TCCGGA 1 cut(s) 29
BspACI CCGC 1 cut(s) 135
BspANI GGCC 2 cut(s) 166, 190
BspCNI CTCAG 1 cut(s) 51
BspDI ATCGAT 2 cut(s) 435, 507
BspEI TCCGGA 1 cut(s) 29
BspFNI CGCG 1 cut(s) 113
BspHI TCATGA 2 cut(s) 537, 589
BspLI GGNNCC 1 cut(s) 165
BspMI ACCTGC 1 cut(s) 133
BspPI GGATC 2 cut(s) 47, 270
BspQI GCTCTTC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 677
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 285, 690
BssNI GRCGYC 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 691
Bst4CI ACNGT 2 cut(s) 286, 575
Bst6I CTCTTC 2 cut(s) 80, 101
BstACI GRCGYC 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 115, 119
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 1 cut(s) 285
BstFNI CGCG 1 cut(s) 113
BstHHI GCGC 2 cut(s) 113, 142
BstNI CCWGG 1 cut(s) 691
BstNSI RCATGY 1 cut(s) 683
BstSCI CCNGG 1 cut(s) 689
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 1 cut(s) 137
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 62
BstXI CCANNNNNNTGG 1 cut(s) 286
BstYI RGATCY 1 cut(s) 62
Bsu15I ATCGAT 2 cut(s) 435, 507
BsuI GTATCC 1 cut(s) 223
BsuRI GGCC 2 cut(s) 166, 190
BsuTUI ATCGAT 2 cut(s) 435, 507
BtgI CCRYGG 1 cut(s) 285
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 2 cut(s) 115, 119
CciI TCATGA 2 cut(s) 537, 589
CfoI GCGC 2 cut(s) 113, 142
Cfr13I GGNCC 1 cut(s) 164
ClaI ATCGAT 2 cut(s) 435, 507
CseI GACGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 229
CviAII CATG 3 cut(s) 538, 590, 680
CviJI RGCY 7 cut(s) 117, 121, 166, 190, 203, 317, 648
CviKI_1 RGCY 7 cut(s) 117, 121, 166, 190, 203, 317, 648
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 1 cut(s) 59
EaeI YGGCCR 1 cut(s) 188
Eam1104I CTCTTC 2 cut(s) 80, 101
EarI CTCTTC 2 cut(s) 80, 101
Eco57I CTGAAG 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 689
FaeI CATG 3 cut(s) 541, 593, 683
FaiI YATR 8 cut(s) 320, 404, 440, 476, 512, 539, 591, 681
FatI CATG 3 cut(s) 537, 589, 679
FbaI TGATCA 5 cut(s) 337, 382, 454, 526, 598
Fnu4HI GCNGC 1 cut(s) 151
Fsp4HI GCNGC 1 cut(s) 151
FspI TGCGCA 1 cut(s) 141
GlaI GCGC 2 cut(s) 112, 141
GluI GCNGC 1 cut(s) 151
GsaI CCCAGC 1 cut(s) 203
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 2 cut(s) 166, 190
HapII CCGG 2 cut(s) 30, 191
HgaI GACGC 1 cut(s) 215
HhaI GCGC 2 cut(s) 113, 142
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 3 cut(s) 541, 593, 683
Hin6I GCGC 2 cut(s) 111, 140
HinP1I GCGC 2 cut(s) 111, 140
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 1 cut(s) 127
HpaII CCGG 2 cut(s) 30, 191
HphI GGTGA 6 cut(s) 16, 214, 335, 455, 527, 635
Hpy166II GTNNAC 2 cut(s) 96, 271
Hpy188I TCNGA 7 cut(s) 53, 60, 84, 102, 393, 465, 609
Hpy188III TCNNGA 6 cut(s) 30, 124, 347, 538, 590, 653
Hpy8I GTNNAC 2 cut(s) 96, 271
Hpy99I CGWCG 1 cut(s) 209
HpyCH4III ACNGT 2 cut(s) 286, 575
HpyCH4V TGCA 2 cut(s) 153, 240
HpyF3I CTNAG 1 cut(s) 59
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 3 cut(s) 541, 593, 683
HspAI GCGC 2 cut(s) 111, 140
Kpn2I TCCGGA 1 cut(s) 29
Ksp22I TGATCA 5 cut(s) 337, 382, 454, 526, 598
LguI GCTCTTC 1 cut(s) 101
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 1 cut(s) 137
MaeIII GTNAC 1 cut(s) 4
MboII GAAGA 7 cut(s) 97, 115, 118, 311, 374, 428, 500
MflI RGATCY 1 cut(s) 62
MluCI AATT 5 cut(s) 78, 386, 458, 530, 602
MnlI CCTC 3 cut(s) 175, 259, 352
MroI TCCGGA 1 cut(s) 29
MseI TTAA 1 cut(s) 639
MslI CAYNNNNRTG 1 cut(s) 158
MspI CCGG 2 cut(s) 30, 191
MspR9I CCNGG 1 cut(s) 691
MvaI CCWGG 1 cut(s) 691
MvnI CGCG 1 cut(s) 113
NlaIII CATG 3 cut(s) 541, 593, 683
NlaIV GGNNCC 1 cut(s) 165
NmuCI GTSAC 1 cut(s) 4
NsbI TGCGCA 1 cut(s) 141
NspI RCATGY 1 cut(s) 683
PaeR7I CTCGAG 1 cut(s) 122
PagI TCATGA 2 cut(s) 537, 589
PciSI GCTCTTC 1 cut(s) 101
PfeI GAWTC 1 cut(s) 127
PkrI GCNGC 1 cut(s) 152
Psp6I CCWGG 1 cut(s) 689
PspFI CCCAGC 1 cut(s) 199
PspGI CCWGG 1 cut(s) 689
PspN4I GGNNCC 1 cut(s) 165
PspPI GGNCC 1 cut(s) 164
PsuI RGATCY 1 cut(s) 62
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 158
SapI GCTCTTC 1 cut(s) 101
SaqAI TTAA 1 cut(s) 639
SatI GCNGC 1 cut(s) 151
Sau96I GGNCC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 691
SetI ASST 5 cut(s) 123, 147, 186, 258, 270
Sfr274I CTCGAG 1 cut(s) 122
SlaI CTCGAG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 5 cut(s) 78, 386, 458, 530, 602
SsiI CCGC 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 689
TaaI ACNGT 2 cut(s) 286, 575
TaqI TCGA 3 cut(s) 123, 435, 507
TasI AATT 5 cut(s) 78, 386, 458, 530, 602
TatI WGTACW 1 cut(s) 228
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 1 cut(s) 639
Tru9I TTAA 1 cut(s) 639
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 1 cut(s) 150
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 554
XceI RCATGY 1 cut(s) 683
XhoI CTCGAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.