RLG00000012128
MADS Family

MADS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011525
Physical Location & Seq
Reverse (-)
15541411 .. 15542241
831 bp
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UTR
Exon/CDS
Intron
RLM00000012128

Sequence Viewer

Length: 831 bp
ATGGTGACGAAATCCCAGACCACCAAATCCGGAGAAAAGATCAAAGAGATCAGAAGTCTGAGATCTTTGGAAAAACCAATTCAGAAGAGAAAGTCCACTCTGAAGAAGAGCGCGAGCCAGCTCGAGATTCTCTGCGGTGCTCAGGTTTGTTGCATTTTGGTGGGGCCAACTGGGAATGTTGAGGTCTGGCCGGAAAACCCAGCCGACGCCGTATCCATTATCACCAAGTACAAACAATGCAAAAAGAAAACAAAGGTTTGTAGGGAGGTCAACCGATCCAAGACCGTGGAGCAGTTTGAAGAAAACAAGGGTTTGGCTATGGGTGATGATGACATTGATCAATCCTTGAATACCCTGGAGAACTTTGAGGAAGACAAGGGGTTTGATATTGATCAATTTCTGAATAACACTATGGAGAACTTTGAAGATCAAAACAAGGGTTTCGATATGGGTGATGATATTGATCAATTTCTCATGAATAACACTATGGAAAATGTTGAACCAAACAACGGTTTGGATACCGATCATGATTTTGATCAATTTCTGAATGATTTTTTTGGTGATACTAGTGATCTTAAAGTGGGCTTTCCAGAGTGTTCAACCAGCAATGCTCACATGCCAACACCAGGGTTTGAGGATCATCAGATTGATAATGTATGTGGAGTGTGGCTGTGGGAGACATTATGGTCCACGCCACAATACTCAGGGTTCGACGGAGCATACTACTTGCCGAAATTTGAGAATGATGACAGTAATATTAGTCTTCTTTGTCAAGAGCAGCCAAAATTGATGGGTTCCAAAAAAACTTTTCGAGATATTGGAAGCAATTAA

Protein Analysis

277

Amino Acids

31.31

Weight (kDa)

4.59

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 28 - 63 9.7e-10 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 685
AccII CGCG 1 cut(s) 113
AccIII TCCGGA 1 cut(s) 29
AciI CCGC 1 cut(s) 135
AclWI GGATC 2 cut(s) 270, 645
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 1 cut(s) 734
AcuI CTGAAG 1 cut(s) 122
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 2 cut(s) 509, 626
AgsI TTSAA 5 cut(s) 299, 349, 425, 500, 600
AhlI ACTAGT 1 cut(s) 566
AjnI CCWGG 2 cut(s) 354, 625
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
Alw21I GWGCWC 1 cut(s) 142
Alw26I GTCTC 1 cut(s) 671
AlwI GGATC 2 cut(s) 270, 645
Ama87I CYCGRG 1 cut(s) 122
Aor13HI TCCGGA 1 cut(s) 29
AoxI GGCC 2 cut(s) 164, 188
ApeKI GCWGC 1 cut(s) 778
ApoI RAATTY 1 cut(s) 734
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 2 cut(s) 164, 687
AsuHPI GGTGA 5 cut(s) 16, 214, 335, 464, 572
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 1 cut(s) 687
BbsI GAAGAC 2 cut(s) 378, 755
Bbv12I GWGCWC 1 cut(s) 142
BbvI GCAGC 1 cut(s) 790
BccI CCATC 1 cut(s) 784
BceAI ACGGC 1 cut(s) 194
BciT130I CCWGG 2 cut(s) 356, 627
BciVI GTATCC 2 cut(s) 223, 511
BclI TGATCA 4 cut(s) 337, 391, 463, 535
BcoDI GTCTC 1 cut(s) 671
BcuI ACTAGT 1 cut(s) 566
BfaI CTAG 1 cut(s) 567
BfuI GTATCC 2 cut(s) 223, 511
BglII AGATCT 1 cut(s) 62
BisI GCNGC 1 cut(s) 779
BlsI GCNGC 1 cut(s) 780
Bme1390I CCNGG 2 cut(s) 356, 627
Bme18I GGWCC 1 cut(s) 687
BmeT110I CYCGRG 1 cut(s) 122
BmgT120I GGNCC 2 cut(s) 164, 687
BmiI GGNNCC 2 cut(s) 165, 796
BmrFI CCNGG 2 cut(s) 356, 627
BmrI ACTGGG 1 cut(s) 180
BmuI ACTGGG 1 cut(s) 180
BpiI GAAGAC 2 cut(s) 378, 755
BpmI CTGGAG 1 cut(s) 377
Bpu10I CCTNAGC 1 cut(s) 141
BsaBI GATNNNNATC 4 cut(s) 390, 462, 522, 534
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 3 cut(s) 285, 354, 626
BsaWI WCCGGW 1 cut(s) 29
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 626
Bse1I ACTGG 1 cut(s) 175
Bse3DI GCAATG 1 cut(s) 613
Bse8I GATNNNNATC 4 cut(s) 390, 462, 522, 534
BseAI TCCGGA 1 cut(s) 29
BseBI CCWGG 2 cut(s) 356, 627
BseDI CCNNGG 3 cut(s) 285, 354, 626
BseJI GATNNNNATC 4 cut(s) 390, 462, 522, 534
BseLI CCNNNNNNNGG 2 cut(s) 509, 626
BseMI GCAATG 1 cut(s) 613
BseMII CTCAG 3 cut(s) 50, 155, 717
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 1 cut(s) 790
BseYI CCCAGC 1 cut(s) 199
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 2 cut(s) 166, 190
BsiHKAI GWGCWC 1 cut(s) 142
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 2 cut(s) 30, 191
BslI CCNNNNNNNGG 2 cut(s) 509, 626
BsmAI GTCTC 1 cut(s) 671
BsnI GGCC 2 cut(s) 166, 190
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 142
Bsp13I TCCGGA 1 cut(s) 29
BspACI CCGC 1 cut(s) 135
BspANI GGCC 2 cut(s) 166, 190
BspCNI CTCAG 3 cut(s) 51, 154, 716
BspEI TCCGGA 1 cut(s) 29
BspFNI CGCG 1 cut(s) 113
BspHI TCATGA 2 cut(s) 474, 526
BspLI GGNNCC 2 cut(s) 165, 796
BspPI GGATC 2 cut(s) 270, 645
BspQI GCTCTTC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 613
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 3 cut(s) 285, 354, 626
BssNI GRCGYC 1 cut(s) 207
Bst2UI CCWGG 2 cut(s) 356, 627
Bst4CI ACNGT 3 cut(s) 286, 512, 752
Bst6I CTCTTC 2 cut(s) 80, 101
BstACI GRCGYC 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 115, 119
BstDEI CTNAG 3 cut(s) 59, 141, 703
BstDSI CCRYGG 1 cut(s) 285
BstFNI CGCG 1 cut(s) 113
BstHHI GCGC 1 cut(s) 113
BstMAI GTCTC 1 cut(s) 671
BstNI CCWGG 2 cut(s) 356, 627
BstNSI RCATGY 1 cut(s) 619
BstSCI CCNGG 2 cut(s) 354, 625
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 1 cut(s) 790
BstV2I GAAGAC 2 cut(s) 378, 755
BstX2I RGATCY 1 cut(s) 62
BstXI CCANNNNNNTGG 1 cut(s) 286
BstYI RGATCY 1 cut(s) 62
BsuI GTATCC 2 cut(s) 223, 511
BsuRI GGCC 2 cut(s) 166, 190
BtgI CCRYGG 1 cut(s) 285
Cac8I GCNNGC 2 cut(s) 115, 119
CciI TCATGA 2 cut(s) 474, 526
CfoI GCGC 1 cut(s) 113
Cfr13I GGNCC 2 cut(s) 164, 687
CseI GACGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 229
CviAII CATG 3 cut(s) 475, 527, 616
CviJI RGCY 9 cut(s) 117, 121, 166, 190, 203, 317, 585, 670, 781
CviKI_1 RGCY 9 cut(s) 117, 121, 166, 190, 203, 317, 585, 670, 781
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 3 cut(s) 59, 141, 703
DrdI GACNNNNNNGTC 1 cut(s) 685
DseDI GACNNNNNNGTC 1 cut(s) 685
EaeI YGGCCR 1 cut(s) 188
Eam1104I CTCTTC 2 cut(s) 80, 101
EarI CTCTTC 2 cut(s) 80, 101
Eco47I GGWCC 1 cut(s) 687
Eco57I CTGAAG 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 122
EcoRII CCWGG 2 cut(s) 354, 625
FaeI CATG 3 cut(s) 478, 530, 619
FatI CATG 3 cut(s) 474, 526, 615
FbaI TGATCA 4 cut(s) 337, 391, 463, 535
Fnu4HI GCNGC 1 cut(s) 779
Fsp4HI GCNGC 1 cut(s) 779
FspBI CTAG 1 cut(s) 567
GlaI GCGC 1 cut(s) 112
GluI GCNGC 1 cut(s) 779
GsaI CCCAGC 1 cut(s) 203
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 2 cut(s) 166, 190
HapII CCGG 2 cut(s) 30, 191
HgaI GACGC 1 cut(s) 215
HhaI GCGC 1 cut(s) 113
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 3 cut(s) 478, 530, 619
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 1 cut(s) 127
HpaII CCGG 2 cut(s) 30, 191
HphI GGTGA 5 cut(s) 16, 214, 335, 464, 572
Hpy166II GTNNAC 3 cut(s) 96, 271, 690
Hpy188I TCNGA 7 cut(s) 53, 60, 84, 102, 402, 546, 645
Hpy188III TCNNGA 7 cut(s) 30, 124, 475, 527, 590, 773, 812
Hpy8I GTNNAC 3 cut(s) 96, 271, 690
Hpy99I CGWCG 2 cut(s) 209, 716
HpyCH4III ACNGT 3 cut(s) 286, 512, 752
HpyCH4V TGCA 2 cut(s) 153, 240
HpyF3I CTNAG 3 cut(s) 59, 141, 703
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 3 cut(s) 478, 530, 619
HspAI GCGC 1 cut(s) 111
Kpn2I TCCGGA 1 cut(s) 29
Ksp22I TGATCA 4 cut(s) 337, 391, 463, 535
LguI GCTCTTC 1 cut(s) 101
LmnI GCTCC 2 cut(s) 289, 716
Lsp1109I GCAGC 1 cut(s) 790
MaeI CTAG 1 cut(s) 567
MaeIII GTNAC 1 cut(s) 4
MboII GAAGA 7 cut(s) 97, 115, 118, 311, 383, 437, 755
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 7 cut(s) 78, 395, 467, 539, 734, 785, 826
MnlI CCTC 4 cut(s) 175, 259, 361, 628
MroI TCCGGA 1 cut(s) 29
MseI TTAA 2 cut(s) 576, 829
MslI CAYNNNNRTG 1 cut(s) 158
MspI CCGG 2 cut(s) 30, 191
MspR9I CCNGG 2 cut(s) 356, 627
MvaI CCWGG 2 cut(s) 356, 627
MvnI CGCG 1 cut(s) 113
NlaIII CATG 3 cut(s) 478, 530, 619
NlaIV GGNNCC 2 cut(s) 165, 796
NmuCI GTSAC 1 cut(s) 4
NspI RCATGY 1 cut(s) 619
PaeR7I CTCGAG 1 cut(s) 122
PagI TCATGA 2 cut(s) 474, 526
PciSI GCTCTTC 1 cut(s) 101
PfeI GAWTC 1 cut(s) 127
PkrI GCNGC 1 cut(s) 780
Psp6I CCWGG 2 cut(s) 354, 625
PspFI CCCAGC 1 cut(s) 199
PspGI CCWGG 2 cut(s) 354, 625
PspN4I GGNNCC 2 cut(s) 165, 796
PspPI GGNCC 2 cut(s) 164, 687
PsuI RGATCY 1 cut(s) 62
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 158
SapI GCTCTTC 1 cut(s) 101
SaqAI TTAA 2 cut(s) 576, 829
SatI GCNGC 1 cut(s) 779
Sau96I GGNCC 2 cut(s) 164, 687
ScrFI CCNGG 2 cut(s) 356, 627
SduI GDGCHC 1 cut(s) 142
SetI ASST 5 cut(s) 123, 147, 186, 258, 270
Sfr274I CTCGAG 1 cut(s) 122
SinI GGWCC 1 cut(s) 687
SlaI CTCGAG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SpeI ACTAGT 1 cut(s) 566
Sse9I AATT 7 cut(s) 78, 395, 467, 539, 734, 785, 826
SsiI CCGC 1 cut(s) 135
SspI AATATT 1 cut(s) 757
SspMI CTAG 1 cut(s) 567
StyD4I CCNGG 2 cut(s) 354, 625
TaaI ACNGT 3 cut(s) 286, 512, 752
TaqI TCGA 4 cut(s) 123, 444, 711, 811
TasI AATT 7 cut(s) 78, 395, 467, 539, 734, 785, 826
TatI WGTACW 1 cut(s) 228
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 2 cut(s) 576, 829
Tru9I TTAA 2 cut(s) 576, 829
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 1 cut(s) 778
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 491
TspGWI ACGGA 1 cut(s) 729
VpaK11BI GGWCC 1 cut(s) 687
XapI RAATTY 1 cut(s) 734
XceI RCATGY 1 cut(s) 619
XhoI CTCGAG 1 cut(s) 122
XspI CTAG 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.