FvH4_2g31102
MADS Family

MADS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011525
Physical Location & Seq
Forward (+)
23938208 .. 23938790
583 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31102.t1

Sequence Viewer

Length: 486 bp
ATGGCTCCCAAGAATTCCAACAATTCATTTGTGAAAAAGCTGCTTGCTGATAACAATGAGTATCTGAAAGCAGCCACCAAGGCTACAAAGTGTCATAAGCTCAAAAACAAGGTCTGGGAGCTTTCAGTCTTGTGCGATGTTATTGTCTCCTTGATTGTTATTGGACCTGATGGAAAAGTATCGAAGGAAAACCTAGACGAGGTGAAATCAATTCTAAGGGAGTACTGGCTAAAAAAGCGACTCGACCGCTCATCGGATCATCTCTTGGAAGTTGTTCTTGAAGAAATCAAGAAGAGGACTGTGGATATCGACCGCAACTGGGTGCCGGCGGAGGAGTTACGGGATATGTGGAATAGTTTGGAGTCCCAGCTAGAGGCAGTGATTGAGAAGACTAGTGGAGTGGTGAGAGATAACGAGACTAGTATTGGTGAAATGAGAAAATCGATGATGCAGATTGATCAATCATTCTCAGATACATCTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

162

Amino Acids

18.5

Weight (kDa)

6.18

Isoelectric Point (pI)

34.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 33 - 60 3.1e-07 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 322
AccBSI CCGCTC 1 cut(s) 249
AciI CCGC 3 cut(s) 247, 313, 329
AclWI GGATC 1 cut(s) 264
AcsI RAATTY 1 cut(s) 13
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 4 cut(s) 199, 253, 319, 373
AgsI TTSAA 1 cut(s) 281
AhlI ACTAGT 2 cut(s) 392, 419
AluBI AGCT 4 cut(s) 40, 100, 121, 370
AluI AGCT 4 cut(s) 40, 100, 121, 370
Alw26I GTCTC 2 cut(s) 151, 410
AlwI GGATC 1 cut(s) 264
ApeKI GCWGC 2 cut(s) 40, 71
ApoI RAATTY 1 cut(s) 13
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
Asp700I GAANNNNTTC 1 cut(s) 273
AspS9I GGNCC 1 cut(s) 164
AsuHPI GGTGA 3 cut(s) 214, 415, 440
AvaII GGWCC 1 cut(s) 164
BanI GGYRCC 1 cut(s) 322
BbsI GAAGAC 1 cut(s) 395
BbvI GCAGC 2 cut(s) 27, 83
BccI CCATC 1 cut(s) 164
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 2 cut(s) 151, 410
BcuI ACTAGT 2 cut(s) 392, 419
BfaI CTAG 4 cut(s) 194, 371, 393, 420
BglI GCCNNNNNGGC 1 cut(s) 80
BisI GCNGC 2 cut(s) 41, 72
BlsI GCNGC 2 cut(s) 42, 73
BmcAI AGTACT 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 164
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 2 cut(s) 6, 324
BmrI ACTGGG 1 cut(s) 328
BmsI GCATC 1 cut(s) 438
BmuI ACTGGG 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 395
Bsa29I ATCGAT 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 78
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 253, 319, 373
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 2 cut(s) 230, 323
BseCI ATCGAT 1 cut(s) 443
BseDI CCNNGG 1 cut(s) 78
BseLI CCNNNNNNNGG 4 cut(s) 199, 253, 319, 373
BseMII CTCAG 1 cut(s) 483
BseNI ACTGG 2 cut(s) 230, 323
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 2 cut(s) 27, 83
BseYI CCCAGC 1 cut(s) 366
Bsh1285I CGRYCG 2 cut(s) 247, 313
BshNI GGYRCC 1 cut(s) 322
BshVI ATCGAT 1 cut(s) 443
BsiEI CGRYCG 2 cut(s) 247, 313
BsiSI CCGG 1 cut(s) 326
BslFI GGGAC 1 cut(s) 349
BslI CCNNNNNNNGG 4 cut(s) 199, 253, 319, 373
BsmAI GTCTC 2 cut(s) 151, 410
BsmFI GGGAC 1 cut(s) 349
Bsp143I GATC 2 cut(s) 256, 457
BspACI CCGC 3 cut(s) 247, 313, 329
BspCNI CTCAG 1 cut(s) 482
BspDI ATCGAT 1 cut(s) 443
BspLI GGNNCC 2 cut(s) 6, 324
BspPI GGATC 1 cut(s) 264
BspT107I GGYRCC 1 cut(s) 322
BsrBI CCGCTC 1 cut(s) 249
BsrFI RCCGGY 1 cut(s) 325
BsrI ACTGG 2 cut(s) 230, 323
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 2 cut(s) 256, 457
BssT1I CCWWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 301
Bst6I CTCTTC 1 cut(s) 287
BstC8I GCNNGC 2 cut(s) 45, 327
BstDEI CTNAG 2 cut(s) 215, 469
BstENI CCTNNNNNAGG 1 cut(s) 197
BstKTI GATC 2 cut(s) 259, 460
BstMAI GTCTC 2 cut(s) 151, 410
BstMBI GATC 2 cut(s) 256, 457
BstMCI CGRYCG 2 cut(s) 247, 313
BstMWI GCNNNNNNNGC 2 cut(s) 80, 235
BstV1I GCAGC 2 cut(s) 27, 83
BstV2I GAAGAC 1 cut(s) 395
Bsu15I ATCGAT 1 cut(s) 443
BsuTUI ATCGAT 1 cut(s) 443
BtgZI GCGATG 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 1 cut(s) 384
Cac8I GCNNGC 2 cut(s) 45, 327
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 1 cut(s) 164
ClaI ATCGAT 1 cut(s) 443
Csp6I GTAC 1 cut(s) 223
CviJI RGCY 8 cut(s) 5, 40, 74, 83, 100, 121, 229, 370
CviKI_1 RGCY 8 cut(s) 5, 40, 74, 83, 100, 121, 229, 370
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 2 cut(s) 215, 469
DpnI GATC 2 cut(s) 258, 459
DpnII GATC 2 cut(s) 256, 457
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
EciI GGCGGA 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 78
Eco32I GATATC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 164
EcoNI CCTNNNNNAGG 1 cut(s) 197
EcoRI GAATTC 1 cut(s) 13
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 78
FaiI YATR 3 cut(s) 96, 347, 482
FalI AAGNNNNNCTT 2 cut(s) 261, 293
FaqI GGGAC 1 cut(s) 349
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 2 cut(s) 41, 72
Fsp4HI GCNGC 2 cut(s) 41, 72
FspBI CTAG 4 cut(s) 194, 371, 393, 420
GluI GCNGC 2 cut(s) 41, 72
GsaI CCCAGC 1 cut(s) 370
HapII CCGG 1 cut(s) 326
HinfI GANTC 2 cut(s) 240, 362
HpaII CCGG 1 cut(s) 326
HphI GGTGA 3 cut(s) 214, 415, 440
Hpy188I TCNGA 3 cut(s) 66, 256, 472
Hpy188III TCNNGA 2 cut(s) 278, 289
HpyAV CCTTC 1 cut(s) 178
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4V TGCA 1 cut(s) 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 235
HpyF3I CTNAG 2 cut(s) 215, 469
KroI GCCGGC 1 cut(s) 325
KroNI GCCGGC 1 cut(s) 327
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 2 cut(s) 256, 457
LmnI GCTCC 2 cut(s) 10, 118
LpnPI CCDG 6 cut(s) 100, 180, 211, 304, 339, 380
Lsp1109I GCAGC 2 cut(s) 27, 83
LweI GCATC 1 cut(s) 438
MaeI CTAG 4 cut(s) 194, 371, 393, 420
MaeIII GTNAC 1 cut(s) 336
MalI GATC 2 cut(s) 258, 459
MbiI CCGCTC 1 cut(s) 249
MboI GATC 2 cut(s) 256, 457
MboII GAAGA 3 cut(s) 293, 304, 400
MluCI AATT 3 cut(s) 13, 22, 210
MlyI GAGTC 2 cut(s) 234, 371
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 4 cut(s) 193, 288, 325, 367
MroNI GCCGGC 1 cut(s) 325
MroXI GAANNNNTTC 1 cut(s) 273
MspI CCGG 1 cut(s) 326
MwoI GCNNNNNNNGC 2 cut(s) 80, 235
NaeI GCCGGC 1 cut(s) 327
NdeII GATC 2 cut(s) 256, 457
NgoMIV GCCGGC 1 cut(s) 325
NlaIV GGNNCC 2 cut(s) 6, 324
PdiI GCCGGC 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 273
PkrI GCNGC 2 cut(s) 42, 73
PleI GAGTC 2 cut(s) 234, 370
PpsI GAGTC 2 cut(s) 234, 370
PspFI CCCAGC 1 cut(s) 366
PspN4I GGNNCC 2 cut(s) 6, 324
PspPI GGNCC 1 cut(s) 164
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SatI GCNGC 2 cut(s) 41, 72
Sau3AI GATC 2 cut(s) 256, 457
Sau96I GGNCC 1 cut(s) 164
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 2 cut(s) 234, 371
SetI ASST 8 cut(s) 42, 102, 114, 123, 169, 195, 204, 372
SfaNI GCATC 1 cut(s) 438
SinI GGWCC 1 cut(s) 164
SpeI ACTAGT 2 cut(s) 392, 419
Sse9I AATT 3 cut(s) 13, 22, 210
SsiI CCGC 3 cut(s) 247, 313, 329
SspMI CTAG 4 cut(s) 194, 371, 393, 420
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 301
TaqI TCGA 4 cut(s) 182, 243, 309, 443
TasI AATT 3 cut(s) 13, 22, 210
TatI WGTACW 1 cut(s) 222
TscAI CASTG 1 cut(s) 384
TseI GCWGC 2 cut(s) 40, 71
TspDTI ATGAA 1 cut(s) 15
TspRI CASTG 1 cut(s) 384
VpaK11BI GGWCC 1 cut(s) 164
XagI CCTNNNNNAGG 1 cut(s) 197
XapI RAATTY 1 cut(s) 13
XmnI GAANNNNTTC 1 cut(s) 273
XspI CTAG 4 cut(s) 194, 371, 393, 420
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.