Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0002616

Physical Location & Seq

Genomic Coordinates Reverse (-)

20002392 .. 20006975

Gene Structure 4584 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g24530.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1791 bp

ATGGGACCTGTTAAGGAAGAGCCGTCGTCATGCAATGCTTTGCAAGTTATTGACTTAGTTTCTGCAGTAAAGGAGTTACACGGGCTTAGCTCTCAGGAACTGGGTAAATTATTGAAGGACTCTGATAATTTTACTATACAGTATGTTACTGAAAAGGAATCACTCCTGAAGATTGACGTTGAAAAGCTTGCCAGTTTTCTCCCTCTGCACCTTATTGCAGTGCTTATGTCATCTGAACAAGATGAAGCCCTATTCAGATACTTATTATGTGGCATTAGGCTCTTGCACTCCTTGTGCGACCTAGCACCTAGAAATCCCAAACTTGAACAGATTTTGCTCGATGATGTAAAAGTGTCAGAACAGCTGCTTGACCTGGTGTTCTATATCCTCATTGTTTTTAGTGGTTATGAACAGAAAAGTAATAATTTTGGTATGGCACCGCTGATGCATTCTGCACTAGTGGCGTGCACTTTACATCTACTTACTGGATGCATATCTTCACAGTGGCAAGATCTTGTTCAAGTATTGCTCGCACATCCTAAGGTTGACATATTTATGGAGGCAGCTTTTGGAGCAGTTTACGCTTCCATTAAGTTTCTCAACCTCATGCTTTCATCTGAACATGAGCAAATAGTTCACTCTCTCTGCCAGCAATGTGAGGCCTCTTTACAGTTTCTCCAATTGCTGTGCCAGCAAAAATTGTTTCGGGAACGACTACTCAGGAATAAGGAACTATGTGGAAAAGGTGGTGTTCTGGTTCTGGCTCAATGCATATTGAAGCTGAACATTGCACCTCATTTAGCATCTGCTAGAATTGTGGCTGCCGTATCTAGACTGAAAGCAAAAATTCTCTCAATTCTGTTAAATCTCTGTGAAGCAGAAAGCATATCTTACCTGGACGAAGTTGCCAGCTCCCCAGGAAGCCTGGATTTGGCAAAGTCTGTTGCATTGGAGATTATTGATTTATTGAAGTGTGCTCTGGGTAAAGATCCCAAATGTTTAGCTGCTGCGGAGGAAACAGATATAGGAGAAGACTTTACCCCCCAAGCTACTGAGGAAATTGACCTAGAGGCATCTGATGCTGATGCTGCAGCAGAAGCAACATCTGTGAGGGCGAAAACAGTTCCGGCGAGTGGTGAAACTTTGAACACTACTTGTTCAGATCCACCTACTGTGTCATCACCCCAAACCTCTATGGAGGTGGCTCTGATGGATGCTATAGAGATGCCCTCCCTTGCTCCAGAGGTGGTTGATGCCAGGAATCTACTAGGTAATTTGGAACAGATCGACCTAGCGGACGAAGAATGCATGCCTCAGATTTCCAAAGCCATAGCGACCCTGTGTTCTTGCGCGAACGAGTCAGAGATGAAGCATCTAGAGGACCTTAAGTTTCGACTTGAACAGATTCCTGATCTTCAAGAAACCTTGCACCAAAAGGAACAAGTCACAAGCGAGTGCCTTCAAAAACAGACAGCCTTAGAGGAGAAGTTTCGGAGCGAAAAGGAACAACTCAACTCTCAGCACAGTTGCATTCTTACTGGAATTGATGCTGAGATCAGATCCTTAGAAAAACAGCGAGCAGATATACAAGCTAAAATCGAGGCTGCTAAACAGAAGAAGACTCAGCATGTACAAGCTGTCAAAACCAAAATTCCCGAGCTCCAGAAACTCAAAGAAACGACCCTTGCTGCTGCTCAGGCAACCAAGATGGCTAAAGATGACGCTGAAGCCAGCCGGACCGACCTAAAGCGTACCTTGCTTGACATCCATCTCCTGGGCAGACTTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006355 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060194 GO:0060195 GO:0060255 GO:0065007 GO:0070013 GO:0080090 GO:0097159 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

597

Amino Acids

65.58

Weight (kDa)

5.02

Isoelectric Point (pI)

47.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nodulin_N	PF25246	12 - 56	4.6e-13	Nodulin N-terminal domain
Nodulin_N	PF25246	98 - 138	7.7e-11	Nodulin N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002616)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03090 AT4G03090 AT4G03090 AT4G03090 AT4G03090
fragaria_vesca	FvH4_2g24490 FvH4_2g24490 FvH4_2g24490 FvH4_2g24490 FvH4_2g24530 FvH4_2g24530 FvH4_5g24052
malus_domestica	MD02G1277900.v1.1
prunus_persica	Prupe.8G250800_v2.0.a1 Prupe.8G267900_v2.0.a1 Prupe.8G267900_v2.0.a1 Prupe.8G267900_v2.0.a1 Prupe.8G267900_v2.0.a1 Prupe.8G267900_v2.0.a1 Prupe.8G267900_v2.0.a1 Prupe.8G267900_v2.0.a1 Prupe.8G267900_v2.0.a1
pyrus_communis	pycom02g23790 pycom07g03300
rosa_chinensis	RchiOBHm_Chr6g0292351
rosa_laevigata	RLG00000012038
rosa_multiflora	Rmu_co8039390.1_g000001 Rmu_sc0001354.1_g000010 Rmu_sc0011092.1_g000002
rosa_roxburghii	Rroxscaffold_7G00175160
rosa_rugosa	Rorug06G0225700 Rorug06G0225700 Rorug06G0225700 Rorug06G0225800 Rorug06G0225800
rosa_samantha	Rh6AG339000 Rh6BG346400 Rh6CG353300 Rh6DG339200
rosa_wichuraiana	Rw6G029510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	436
AccB7I	CCANNNNNTGG	1 cut(s)	1774
AccII	CGCG	1 cut(s)	1352
AciI	CCGC	3 cut(s)	440, 1010, 1295
AclWI	GGATC	3 cut(s)	983, 1157, 1554
AcsI	RAATTY	2 cut(s)	846, 1650
AcuI	CTGAAG	2 cut(s)	188, 1746
AfaI	GTAC	2 cut(s)	1632, 1753
AfiI	CCNNNNNNNGG	3 cut(s)	931, 1133, 1774
AflII	CTTAAG	1 cut(s)	1385
AhlI	ACTAGT	1 cut(s)	457
AjnI	CCWGG	6 cut(s)	372, 894, 916, 924, 1256, 1773
Alw21I	GWGCWC	3 cut(s)	470, 979, 1662
Alw44I	GTGCAC	1 cut(s)	466
AlwI	GGATC	3 cut(s)	983, 1157, 1554
AlwNI	CAGNNNCTG	1 cut(s)	100
Ama87I	CYCGRG	1 cut(s)	1655
AoxI	GGCC	1 cut(s)	660
ApaLI	GTGCAC	1 cut(s)	466
ApoI	RAATTY	2 cut(s)	846, 1650
Asp700I	GAANNNNTTC	1 cut(s)	1404
AspLEI	GCGC	1 cut(s)	1352
AspS9I	GGNCC	3 cut(s)	5, 1381, 1737
AsuHPI	GGTGA	2 cut(s)	1148, 1173
AvaI	CYCGRG	1 cut(s)	1655
AvaII	GGWCC	3 cut(s)	5, 1381, 1737
AxyI	CCTNAGG	1 cut(s)	540
BaeGI	GKGCMC	1 cut(s)	470
BanI	GGYRCC	1 cut(s)	436
BanII	GRGCYC	1 cut(s)	1662
BbsI	GAAGAC	2 cut(s)	1038, 1625
Bbv12I	GWGCWC	3 cut(s)	470, 979, 1662
BccI	CCATC	3 cut(s)	1204, 1702, 1776
BceAI	ACGGC	2 cut(s)	7, 809
BciT130I	CCWGG	6 cut(s)	374, 896, 918, 926, 1258, 1775
BcuI	ACTAGT	1 cut(s)	457
BfaI	CTAG	9 cut(s)	302, 309, 458, 810, 831, 1067, 1268, 1292, 1376
BfmI	CTRYAG	3 cut(s)	63, 1089, 1218
BfrI	CTTAAG	1 cut(s)	1385
BglII	AGATCT	1 cut(s)	511
BlpI	GCTNAGC	1 cut(s)	86
Bme1390I	CCNGG	6 cut(s)	374, 896, 918, 926, 1258, 1775
Bme18I	GGWCC	3 cut(s)	5, 1381, 1737
BmeT110I	CYCGRG	1 cut(s)	1655
BmgT120I	GGNCC	3 cut(s)	5, 1381, 1737
BmiI	GGNNCC	2 cut(s)	6, 438
BmrFI	CCNGG	6 cut(s)	374, 896, 918, 926, 1258, 1775
BmrI	ACTGGG	1 cut(s)	110
BmuI	ACTGGG	1 cut(s)	110
BpiI	GAAGAC	2 cut(s)	1038, 1625
BplI	GAGNNNNNCTC	2 cut(s)	1214, 1246
BpmI	CTGGAG	2 cut(s)	1224, 1646
Bpu10I	CCTNAGC	1 cut(s)	1695
Bpu1102I	GCTNAGC	1 cut(s)	86
BsaBI	GATNNNNATC	1 cut(s)	493
BsaJI	CCNNGG	2 cut(s)	916, 1774
BsaXI	ACNNNNNCTCC	2 cut(s)	660, 690
Bsc4I	CCNNNNNNNGG	3 cut(s)	931, 1133, 1774
Bse1I	ACTGG	4 cut(s)	105, 192, 490, 1543
Bse21I	CCTNAGG	1 cut(s)	540
Bse3DI	GCAATG	3 cut(s)	40, 659, 786
Bse8I	GATNNNNATC	1 cut(s)	493
BseBI	CCWGG	6 cut(s)	374, 896, 918, 926, 1258, 1775
BseDI	CCNNGG	2 cut(s)	916, 1774
BseGI	GGATG	4 cut(s)	494, 535, 1219, 1764
BseJI	GATNNNNATC	1 cut(s)	493
BseLI	CCNNNNNNNGG	3 cut(s)	931, 1133, 1774
BseMI	GCAATG	3 cut(s)	40, 659, 786
BseMII	CTCAG	8 cut(s)	107, 733, 1044, 1328, 1532, 1542, 1637, 1709
BseNI	ACTGG	4 cut(s)	105, 192, 490, 1543
BseRI	GAGGAG	1 cut(s)	1496
BseSI	GKGCMC	1 cut(s)	470
BsgI	GTGCAG	2 cut(s)	191, 438
Bsh1236I	CGCG	1 cut(s)	1352
BshFI	GGCC	1 cut(s)	662
BshNI	GGYRCC	1 cut(s)	436
BsiHKAI	GWGCWC	3 cut(s)	470, 979, 1662
BsiHKCI	CYCGRG	1 cut(s)	1655
BsiSI	CCGG	2 cut(s)	1127, 1735
BslFI	GGGAC	1 cut(s)	18
BslI	CCNNNNNNNGG	3 cut(s)	931, 1133, 1774
BsmFI	GGGAC	1 cut(s)	18
BsmI	GAATGC	3 cut(s)	448, 1310, 1530
BsnI	GGCC	1 cut(s)	662
BsoBI	CYCGRG	1 cut(s)	1655
Bsp1286I	GDGCHC	3 cut(s)	470, 979, 1662
Bsp1407I	TGTACA	1 cut(s)	1630
Bsp143I	GATC	7 cut(s)	511, 988, 1162, 1284, 1411, 1554, 1559
Bsp1720I	GCTNAGC	1 cut(s)	86
BspACI	CCGC	3 cut(s)	440, 1010, 1295
BspANI	GGCC	1 cut(s)	662
BspCNI	CTCAG	8 cut(s)	106, 732, 1045, 1327, 1531, 1543, 1636, 1708
BspFNI	CGCG	1 cut(s)	1352
BspLI	GGNNCC	2 cut(s)	6, 438
BspMAI	CTGCAG	2 cut(s)	67, 1093
BspPI	GGATC	3 cut(s)	983, 1157, 1554
BspQI	GCTCTTC	1 cut(s)	12
BspT107I	GGYRCC	1 cut(s)	436
BspTI	CTTAAG	1 cut(s)	1385
BsrDI	GCAATG	3 cut(s)	40, 659, 786
BsrGI	TGTACA	1 cut(s)	1630
BsrI	ACTGG	4 cut(s)	105, 192, 490, 1543
BssECI	CCNNGG	2 cut(s)	916, 1774
BssMI	GATC	7 cut(s)	511, 988, 1162, 1284, 1411, 1554, 1559
Bst2UI	CCWGG	6 cut(s)	374, 896, 918, 926, 1258, 1775
Bst4CI	ACNGT	6 cut(s)	141, 504, 672, 1123, 1174, 1526
Bst6I	CTCTTC	1 cut(s)	12
BstAFI	CTTAAG	1 cut(s)	1385
BstAPI	GCANNNNNTGC	1 cut(s)	1079
BstAUI	TGTACA	1 cut(s)	1630
BstC8I	GCNNGC	9 cut(s)	189, 466, 531, 650, 692, 910, 1310, 1578, 1732
BstF5I	GGATG	4 cut(s)	494, 535, 1219, 1764
BstFNI	CGCG	1 cut(s)	1352
BstHHI	GCGC	1 cut(s)	1352
BstKTI	GATC	7 cut(s)	514, 991, 1165, 1287, 1414, 1557, 1562
BstMBI	GATC	7 cut(s)	511, 988, 1162, 1284, 1411, 1554, 1559
BstMWI	GCNNNNNNNGC	9 cut(s)	461, 572, 581, 691, 1079, 1088, 1097, 1697, 1756
BstNI	CCWGG	6 cut(s)	374, 896, 918, 926, 1258, 1775
BstNSI	RCATGY	2 cut(s)	1312, 1631
BstSCI	CCNGG	6 cut(s)	372, 894, 916, 924, 1256, 1773
BstSFI	CTRYAG	3 cut(s)	63, 1089, 1218
BstSLI	GKGCMC	1 cut(s)	470
BstUI	CGCG	1 cut(s)	1352
BstV2I	GAAGAC	2 cut(s)	1038, 1625
BstX2I	RGATCY	4 cut(s)	511, 988, 1162, 1559
BstYI	RGATCY	4 cut(s)	511, 988, 1162, 1559
Bsu36I	CCTNAGG	1 cut(s)	540
BsuRI	GGCC	1 cut(s)	662
BtsCI	GGATG	4 cut(s)	494, 535, 1219, 1764
BtsI	GCAGTG	1 cut(s)	225
BtsIMutI	CAGTG	2 cut(s)	225, 509
Cac8I	GCNNGC	9 cut(s)	189, 466, 531, 650, 692, 910, 1310, 1578, 1732
CaiI	CAGNNNCTG	1 cut(s)	100
CfoI	GCGC	1 cut(s)	1352
Cfr13I	GGNCC	3 cut(s)	5, 1381, 1737
CpoI	CGGWCCG	1 cut(s)	1737
CseI	GACGC	1 cut(s)	1730
CsiI	ACCWGGT	1 cut(s)	372
Csp6I	GTAC	2 cut(s)	1631, 1752
CspI	CGGWCCG	1 cut(s)	1737
CviAII	CATG	5 cut(s)	30, 607, 623, 1309, 1628
CviQI	GTAC	2 cut(s)	1631, 1752
DpnI	GATC	7 cut(s)	513, 990, 1164, 1286, 1413, 1556, 1561
DpnII	GATC	7 cut(s)	511, 988, 1162, 1284, 1411, 1554, 1559
Eam1104I	CTCTTC	1 cut(s)	12
EarI	CTCTTC	1 cut(s)	12
Ecl136II	GAGCTC	1 cut(s)	1660
Eco147I	AGGCCT	1 cut(s)	662
Eco24I	GRGCYC	1 cut(s)	1662
Eco47I	GGWCC	3 cut(s)	5, 1381, 1737
Eco53kI	GAGCTC	1 cut(s)	1660
Eco57I	CTGAAG	2 cut(s)	188, 1746
Eco81I	CCTNAGG	1 cut(s)	540
Eco88I	CYCGRG	1 cut(s)	1655
EcoICRI	GAGCTC	1 cut(s)	1660
EcoO109I	RGGNCCY	2 cut(s)	5, 1381
EcoRII	CCWGG	6 cut(s)	372, 894, 916, 924, 1256, 1773
EcoT22I	ATGCAT	4 cut(s)	450, 494, 773, 1310
EcoT38I	GRGCYC	1 cut(s)	1662
FaeI	CATG	5 cut(s)	33, 610, 626, 1312, 1631
FalI	AAGNNNNNCTT	4 cut(s)	874, 906, 1739, 1771
FaqI	GGGAC	1 cut(s)	18
FatI	CATG	5 cut(s)	29, 606, 622, 1308, 1627
FokI	GGATG	4 cut(s)	501, 522, 1226, 1751
FriOI	GRGCYC	1 cut(s)	1662
FspBI	CTAG	9 cut(s)	302, 309, 458, 810, 831, 1067, 1268, 1292, 1376
GlaI	GCGC	1 cut(s)	1351
GsuI	CTGGAG	2 cut(s)	1224, 1646
HaeIII	GGCC	1 cut(s)	662
HapII	CCGG	2 cut(s)	1127, 1735
HgaI	GACGC	1 cut(s)	1730
HhaI	GCGC	1 cut(s)	1352
Hin1II	CATG	5 cut(s)	33, 610, 626, 1312, 1631
Hin6I	GCGC	1 cut(s)	1350
HinP1I	GCGC	1 cut(s)	1350
HincII	GTYRAC	1 cut(s)	547
HindII	GTYRAC	1 cut(s)	547
HindIII	AAGCTT	1 cut(s)	185
HinfI	GANTC	6 cut(s)	119, 158, 1261, 1358, 1405, 1621
HpaII	CCGG	2 cut(s)	1127, 1735
HphI	GGTGA	2 cut(s)	1148, 1173
Hpy166II	GTNNAC	4 cut(s)	468, 547, 580, 637
Hpy8I	GTNNAC	4 cut(s)	468, 547, 580, 637
Hpy99I	CGWCG	1 cut(s)	28
HpyAV	CCTTC	2 cut(s)	109, 1469
HpyCH4III	ACNGT	6 cut(s)	141, 504, 672, 1123, 1174, 1526
HpyCH4IV	ACGT	1 cut(s)	177
HpyF10VI	GCNNNNNNNGC	9 cut(s)	461, 572, 581, 691, 1079, 1088, 1097, 1697, 1756
HpySE526I	ACGT	1 cut(s)	177
Hsp92II	CATG	5 cut(s)	33, 610, 626, 1312, 1631
HspAI	GCGC	1 cut(s)	1350
Kzo9I	GATC	7 cut(s)	511, 988, 1162, 1284, 1411, 1554, 1559
LguI	GCTCTTC	1 cut(s)	12
LmnI	GCTCC	5 cut(s)	572, 917, 1243, 1494, 1665
MabI	ACCWGGT	1 cut(s)	372
MaeI	CTAG	9 cut(s)	302, 309, 458, 810, 831, 1067, 1268, 1292, 1376
MaeII	ACGT	1 cut(s)	177
MaeIII	GTNAC	3 cut(s)	75, 145, 1444
MalI	GATC	7 cut(s)	513, 990, 1164, 1286, 1413, 1556, 1561
MboI	GATC	7 cut(s)	511, 988, 1162, 1284, 1411, 1554, 1559
MboII	GAAGA	8 cut(s)	29, 181, 489, 1043, 1313, 1406, 1627, 1630
MfeI	CAATTG	1 cut(s)	680
MflI	RGATCY	4 cut(s)	511, 988, 1162, 1559
MhlI	GDGCHC	3 cut(s)	470, 979, 1662
MlyI	GAGTC	3 cut(s)	113, 1367, 1615
Mph1103I	ATGCAT	4 cut(s)	450, 494, 773, 1310
MroXI	GAANNNNTTC	1 cut(s)	1404
MseI	TTAA	4 cut(s)	12, 591, 863, 1386
MslI	CAYNNNNRTG	1 cut(s)	554
MspA1I	CMGCKG	2 cut(s)	364, 442
MspCI	CTTAAG	1 cut(s)	1385
MspI	CCGG	2 cut(s)	1127, 1735
MspR9I	CCNGG	6 cut(s)	374, 896, 918, 926, 1258, 1775
MunI	CAATTG	1 cut(s)	680
Mva1269I	GAATGC	3 cut(s)	448, 1310, 1530
MvaI	CCWGG	6 cut(s)	374, 896, 918, 926, 1258, 1775
MvnI	CGCG	1 cut(s)	1352
MwoI	GCNNNNNNNGC	9 cut(s)	461, 572, 581, 691, 1079, 1088, 1097, 1697, 1756
NdeII	GATC	7 cut(s)	511, 988, 1162, 1284, 1411, 1554, 1559
NlaIII	CATG	5 cut(s)	33, 610, 626, 1312, 1631
NlaIV	GGNNCC	2 cut(s)	6, 438
NmuCI	GTSAC	1 cut(s)	1444
NsiI	ATGCAT	4 cut(s)	450, 494, 773, 1310
NspI	RCATGY	2 cut(s)	1312, 1631
PaeI	GCATGC	1 cut(s)	1312
PceI	AGGCCT	1 cut(s)	662
PciSI	GCTCTTC	1 cut(s)	12
PctI	GAATGC	3 cut(s)	448, 1310, 1530
PdmI	GAANNNNTTC	1 cut(s)	1404
PfeI	GAWTC	3 cut(s)	158, 1261, 1405
PflMI	CCANNNNNTGG	1 cut(s)	1774
PleI	GAGTC	3 cut(s)	113, 1366, 1615
PpsI	GAGTC	3 cut(s)	113, 1366, 1615
PpuMI	RGGWCCY	2 cut(s)	5, 1381
Psp124BI	GAGCTC	1 cut(s)	1662
Psp5II	RGGWCCY	2 cut(s)	5, 1381
Psp6I	CCWGG	6 cut(s)	372, 894, 916, 924, 1256, 1773
PspGI	CCWGG	6 cut(s)	372, 894, 916, 924, 1256, 1773
PspN4I	GGNNCC	2 cut(s)	6, 438
PspPI	GGNCC	3 cut(s)	5, 1381, 1737
PspPPI	RGGWCCY	2 cut(s)	5, 1381
PstI	CTGCAG	2 cut(s)	67, 1093
PstNI	CAGNNNCTG	1 cut(s)	100
PsuI	RGATCY	4 cut(s)	511, 988, 1162, 1559
PvuII	CAGCTG	1 cut(s)	364
RsaI	GTAC	2 cut(s)	1632, 1753
RsaNI	GTAC	2 cut(s)	1631, 1752
RseI	CAYNNNNRTG	1 cut(s)	554
Rsr2I	CGGWCCG	1 cut(s)	1737
RsrII	CGGWCCG	1 cut(s)	1737
SacI	GAGCTC	1 cut(s)	1662
SapI	GCTCTTC	1 cut(s)	12
SaqAI	TTAA	4 cut(s)	12, 591, 863, 1386
Sau3AI	GATC	7 cut(s)	511, 988, 1162, 1284, 1411, 1554, 1559
Sau96I	GGNCC	3 cut(s)	5, 1381, 1737
SchI	GAGTC	3 cut(s)	113, 1367, 1615
ScrFI	CCNGG	6 cut(s)	374, 896, 918, 926, 1258, 1775
SduI	GDGCHC	3 cut(s)	470, 979, 1662
SexAI	ACCWGGT	1 cut(s)	372
SfcI	CTRYAG	3 cut(s)	63, 1089, 1218
SinI	GGWCC	3 cut(s)	5, 1381, 1737
SmiMI	CAYNNNNRTG	1 cut(s)	554
SmlI	CTYRAG	1 cut(s)	1385
SmoI	CTYRAG	1 cut(s)	1385
SpeI	ACTAGT	1 cut(s)	457
SphI	GCATGC	1 cut(s)	1312
SseBI	AGGCCT	1 cut(s)	662
SsiI	CCGC	3 cut(s)	440, 1010, 1295
SspMI	CTAG	9 cut(s)	302, 309, 458, 810, 831, 1067, 1268, 1292, 1376
SstI	GAGCTC	1 cut(s)	1662
StuI	AGGCCT	1 cut(s)	662
StyD4I	CCNGG	6 cut(s)	372, 894, 916, 924, 1256, 1773
TaaI	ACNGT	6 cut(s)	141, 504, 672, 1123, 1174, 1526
TaiI	ACGT	1 cut(s)	180
TaqI	TCGA	4 cut(s)	339, 1287, 1393, 1599
TaqII	GACCGA	1 cut(s)	1754
TatI	WGTACW	1 cut(s)	1630
TfiI	GAWTC	3 cut(s)	158, 1261, 1405
Tru1I	TTAA	4 cut(s)	12, 591, 863, 1386
Tru9I	TTAA	4 cut(s)	12, 591, 863, 1386
TscAI	CASTG	2 cut(s)	225, 509
TseFI	GTSAC	1 cut(s)	1444
Tsp45I	GTSAC	1 cut(s)	1444
TspDTI	ATGAA	4 cut(s)	258, 423, 603, 1382
TspRI	CASTG	2 cut(s)	225, 509
Van91I	CCANNNNNTGG	1 cut(s)	1774
Vha464I	CTTAAG	1 cut(s)	1385
VneI	GTGCAC	1 cut(s)	466
VpaK11BI	GGWCC	3 cut(s)	5, 1381, 1737
XapI	RAATTY	2 cut(s)	846, 1650
XbaI	TCTAGA	2 cut(s)	830, 1375
XceI	RCATGY	2 cut(s)	1312, 1631
XmnI	GAANNNNTTC	1 cut(s)	1404
XspI	CTAG	9 cut(s)	302, 309, 458, 810, 831, 1067, 1268, 1292, 1376
Zsp2I	ATGCAT	4 cut(s)	450, 494, 773, 1310

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.