FvH4_2g26150

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005900
Physical Location & Seq
Forward (+)
21023305 .. 21026419
3115 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26150.t3

Sequence Viewer

Length: 1413 bp
ATGAAGAGGCTAGTAAGTAGGCATCCAACTGTGGTTCAACCTTTTCTTCTTGTTTTTTGTTATTTGCAGTTGGCGCTGGTTTTGGTTCTGGGATATAGTAGTGCATTACAGTATGAAGGAGAATGTGACAACCAGAAGGCATTGAATCCCAGGCCTCACAGTGTGTCGCTTTTGGAGTTTGGGGCAGTTGGAGATGGGAAAACATTGAACACAGTTGCTTTCCAGAATGCCGTTTTTTATGTCAAGTCATTTGCTGACAAGGGTGGTGCTCAGCTTTATGTTCCGCCTGGCCGGTGGCTCACTGGGAGCTTTTACCTCACTAGCCACCTCACGCTCTTCATTGAGACCGGTGCGACTATTCTTGGATCCCAGGACTACACTCACTGGGGAATTGAGGAACCCCTTCCTTCTTATGGTCGCGGGATTGATTTGCCAGGTTGGAGATATCGCAGCTTGATCAACGGAGATGATCTACACGACGTGGTGATAACAGGTGGTAATGGAACCATAGATGGACAGGGTTCTGTGTGGTGGGCGCAATTCAACTCTCATTCCTTGAATTACAGCAGACCACATCTCGTGGAATTCACCAGATGTGATAATGTCGTTATATCGAATCTGACTTTCCTGAACTCCCCTGCTTGGAACATTCATCCTACATATTGCAGGTATGTGAAAGTCCAAAACATAACTCTTCATGCTTATCCTGCGTCTCCATATACTAGTGGTATAGTCCCAGATTCGTCTATGCATGTTTGCATTGAGAACTGCAACATTAGCATGGGTCATGATGCGATTGTTCTCAAGAGCGGTTGGGATGAGTATGGTATCGCCTATGGCAAACCGACCAAAAGTGTACACATTCGTGGCGTCAACCTTCAATCTTCTTTAGGCTCCGGCCTAGCATTTGGGAGTGAGATGTCTGGAGGCATATTAAGCGTTCTAGTAGAACGACTCCATCTTTCTGACTCATTCATTGGCATTGCACTCAAGACAACTAGAGGAAGAGGAGGATATATGAAAGACATAGTCATATCTAATGTAGAAATGGAAAACATTCACACTGCAATACAAGTTACTGGTCAGAGTGGCACTCATCCTGATGATAAATTTGACCCTGATCAACTTCCATTTGTTAAAGGAATCACCTTTGACAACATGGTTGGCAGTAATATTACTTTTGCTGGGAATTTTTCTGGAATCAACAAATCGCCTTTCACATCTATTTGTCTCTCAAACGTGTCATTCTCCATGACTGGAAATTCTTCTAGCTCATGGTTCTGTCGCGATGTGAAGGGGTTCTCCCAGAAAGTCTCTCCGGAACCTTGTTCAGACCTCAAGGACTTGTCTTTTAATGCTTCCTCATCTTGCTTCTTTTCCTTACAAACTTATAATTATGGTACAGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

471

Amino Acids

51.53

Weight (kDa)

6.19

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 133 - 413 8.5e-30 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1392
Acc36I ACCTGC 1 cut(s) 657
AccBSI CCGCTC 1 cut(s) 810
AccII CGCG 2 cut(s) 420, 1287
AccIII TCCGGA 1 cut(s) 1318
AciI CCGC 3 cut(s) 284, 420, 810
AclWI GGATC 2 cut(s) 360, 373
AcoI YGGCCR 1 cut(s) 289
AcsI RAATTY 4 cut(s) 584, 1109, 1189, 1261
AcyI GRCGYC 1 cut(s) 870
AdeI CACNNNGTG 2 cut(s) 163, 481
AfaI GTAC 2 cut(s) 858, 1402
AfiI CCNNNNNNNGG 2 cut(s) 413, 642
AflIII ACRYGT 1 cut(s) 1239
AgeI ACCGGT 1 cut(s) 347
AgsI TTSAA 6 cut(s) 38, 145, 208, 544, 559, 881
AhlI ACTAGT 1 cut(s) 722
AjiI CACGTC 1 cut(s) 481
AjnI CCWGG 4 cut(s) 149, 286, 369, 433
AleI CACNNNNGTG 1 cut(s) 864
AluBI AGCT 4 cut(s) 274, 309, 453, 1272
AluI AGCT 4 cut(s) 274, 309, 453, 1272
Alw21I GWGCWC 1 cut(s) 271
Alw26I GTCTC 4 cut(s) 338, 717, 1235, 1318
AlwI GGATC 2 cut(s) 360, 373
Aor13HI TCCGGA 1 cut(s) 1318
AoxI GGCC 3 cut(s) 152, 289, 898
ApeKI GCWGC 1 cut(s) 450
ApoI RAATTY 4 cut(s) 584, 1109, 1189, 1261
AsiGI ACCGGT 1 cut(s) 347
Asp700I GAANNNNTTC 4 cut(s) 402, 1056, 1264, 1298
AspLEI GCGC 2 cut(s) 76, 538
AsuHPI GGTGA 3 cut(s) 496, 580, 1138
BamHI GGATCC 1 cut(s) 365
BauI CACGAG 1 cut(s) 578
Bbv12I GWGCWC 1 cut(s) 271
BbvI GCAGC 1 cut(s) 462
BccI CCATC 3 cut(s) 188, 506, 966
BceAI ACGGC 1 cut(s) 215
BciT130I CCWGG 4 cut(s) 151, 288, 371, 435
BclI TGATCA 2 cut(s) 456, 1120
BcoDI GTCTC 4 cut(s) 338, 717, 1235, 1318
BcuI ACTAGT 1 cut(s) 722
BfaI CTAG 7 cut(s) 11, 321, 723, 902, 944, 999, 1269
BfoI RGCGCY 1 cut(s) 77
BfuAI ACCTGC 1 cut(s) 657
BisI GCNGC 1 cut(s) 451
BlpI GCTNAGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 452
Bme1390I CCNGG 4 cut(s) 151, 288, 371, 435
BmgBI CACGTC 1 cut(s) 481
BmiI GGNNCC 5 cut(s) 367, 399, 505, 895, 1323
BmrFI CCNGG 4 cut(s) 151, 288, 371, 435
BmrI ACTGGG 2 cut(s) 312, 394
BmsI GCATC 2 cut(s) 31, 781
BmuI ACTGGG 2 cut(s) 312, 394
BplI GAGNNNNNCTC 2 cut(s) 1078, 1110
BpmI CTGGAG 1 cut(s) 945
Bpu1102I GCTNAGC 1 cut(s) 270
BpuEI CTTGAG 3 cut(s) 788, 974, 1322
BsaHI GRCGYC 1 cut(s) 870
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 2 cut(s) 149, 369
BsaWI WCCGGW 2 cut(s) 347, 1318
Bsc4I CCNNNNNNNGG 2 cut(s) 413, 642
Bse118I RCCGGY 2 cut(s) 291, 347
Bse1I ACTGG 4 cut(s) 307, 389, 1084, 1261
Bse3DI GCAATG 1 cut(s) 981
BseAI TCCGGA 1 cut(s) 1318
BseBI CCWGG 4 cut(s) 151, 288, 371, 435
BseDI CCNNGG 2 cut(s) 149, 369
BseGI GGATG 4 cut(s) 22, 652, 823, 1096
BseLI CCNNNNNNNGG 2 cut(s) 413, 642
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 4 cut(s) 307, 389, 1084, 1261
BseRI GAGGAG 1 cut(s) 1023
BseXI GCAGC 1 cut(s) 462
BseYI CCCAGC 1 cut(s) 1184
Bsh1236I CGCG 2 cut(s) 420, 1287
BshFI GGCC 3 cut(s) 154, 291, 900
BshTI ACCGGT 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 271
BsiSI CCGG 4 cut(s) 292, 348, 897, 1319
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 2 cut(s) 413, 642
BsmAI GTCTC 4 cut(s) 338, 717, 1235, 1318
BsmBI CGTCTC 1 cut(s) 717
BsmFI GGGAC 1 cut(s) 719
BsmI GAATGC 1 cut(s) 232
BsnI GGCC 3 cut(s) 154, 291, 900
Bso31I GGTCTC 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 271
Bsp13I TCCGGA 1 cut(s) 1318
Bsp1407I TGTACA 1 cut(s) 856
Bsp143I GATC 4 cut(s) 365, 456, 469, 1120
Bsp1720I GCTNAGC 1 cut(s) 270
Bsp68I TCGCGA 1 cut(s) 1287
BspACI CCGC 3 cut(s) 284, 420, 810
BspANI GGCC 3 cut(s) 154, 291, 900
BspCNI CTCAG 1 cut(s) 283
BspEI TCCGGA 1 cut(s) 1318
BspFNI CGCG 2 cut(s) 420, 1287
BspHI TCATGA 1 cut(s) 787
BspLI GGNNCC 5 cut(s) 367, 399, 505, 895, 1323
BspMI ACCTGC 1 cut(s) 657
BspPI GGATC 2 cut(s) 360, 373
BspQI GCTCTTC 1 cut(s) 341
BspTNI GGTCTC 1 cut(s) 338
BsrBI CCGCTC 1 cut(s) 810
BsrDI GCAATG 1 cut(s) 981
BsrFI RCCGGY 2 cut(s) 291, 347
BsrGI TGTACA 1 cut(s) 856
BsrI ACTGG 4 cut(s) 307, 389, 1084, 1261
BssAI RCCGGY 2 cut(s) 291, 347
BssECI CCNNGG 2 cut(s) 149, 369
BssMI GATC 4 cut(s) 365, 456, 469, 1120
BssNI GRCGYC 1 cut(s) 870
BssSI CACGAG 1 cut(s) 578
Bst2BI CACGAG 1 cut(s) 578
Bst2UI CCWGG 4 cut(s) 151, 288, 371, 435
Bst4CI ACNGT 4 cut(s) 31, 111, 161, 214
Bst6I CTCTTC 3 cut(s) 341, 699, 1000
BstACI GRCGYC 1 cut(s) 870
BstAUI TGTACA 1 cut(s) 856
BstDEI CTNAG 1 cut(s) 270
BstF5I GGATG 4 cut(s) 22, 652, 823, 1096
BstFNI CGCG 2 cut(s) 420, 1287
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 2 cut(s) 76, 538
BstKTI GATC 4 cut(s) 368, 459, 472, 1123
BstMAI GTCTC 4 cut(s) 338, 717, 1235, 1318
BstMBI GATC 4 cut(s) 365, 456, 469, 1120
BstMWI GCNNNNNNNGC 4 cut(s) 73, 707, 777, 936
BstNI CCWGG 4 cut(s) 151, 288, 371, 435
BstNSI RCATGY 1 cut(s) 755
BstSCI CCNGG 4 cut(s) 149, 286, 369, 433
BstUI CGCG 2 cut(s) 420, 1287
BstV1I GCAGC 1 cut(s) 462
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
BsuRI GGCC 3 cut(s) 154, 291, 900
BtgZI GCGATG 1 cut(s) 1302
BtrI CACGTC 1 cut(s) 481
BtsCI GGATG 4 cut(s) 22, 652, 823, 1096
BtsI GCAGTG 1 cut(s) 1062
BtsIMutI CAGTG 4 cut(s) 166, 300, 382, 1062
BtuMI TCGCGA 1 cut(s) 1287
BveI ACCTGC 1 cut(s) 657
CciI TCATGA 1 cut(s) 787
CfoI GCGC 2 cut(s) 76, 538
Cfr10I RCCGGY 2 cut(s) 291, 347
CseI GACGC 2 cut(s) 699, 859
Csp6I GTAC 2 cut(s) 857, 1401
CspAI ACCGGT 1 cut(s) 347
CviAII CATG 7 cut(s) 698, 752, 781, 788, 1159, 1252, 1275
CviQI GTAC 2 cut(s) 857, 1401
DdeI CTNAG 1 cut(s) 270
DpnI GATC 4 cut(s) 367, 458, 471, 1122
DpnII GATC 4 cut(s) 365, 456, 469, 1120
DraIII CACNNNGTG 2 cut(s) 163, 481
EaeI YGGCCR 1 cut(s) 289
Eam1104I CTCTTC 3 cut(s) 341, 699, 1000
EarI CTCTTC 3 cut(s) 341, 699, 1000
EciI GGCGGA 1 cut(s) 273
Eco147I AGGCCT 1 cut(s) 154
Eco31I GGTCTC 1 cut(s) 338
Eco32I GATATC 1 cut(s) 446
EcoRI GAATTC 1 cut(s) 584
EcoRII CCWGG 4 cut(s) 149, 286, 369, 433
EcoRV GATATC 1 cut(s) 446
EcoT22I ATGCAT 1 cut(s) 753
Esp3I CGTCTC 1 cut(s) 717
FaeI CATG 7 cut(s) 701, 755, 784, 791, 1162, 1255, 1278
FaqI GGGAC 1 cut(s) 719
FatI CATG 7 cut(s) 697, 751, 780, 787, 1158, 1251, 1274
FauI CCCGC 1 cut(s) 413
FbaI TGATCA 2 cut(s) 456, 1120
Fnu4HI GCNGC 1 cut(s) 451
FokI GGATG 4 cut(s) 9, 639, 830, 1083
Fsp4HI GCNGC 1 cut(s) 451
FspBI CTAG 7 cut(s) 11, 321, 723, 902, 944, 999, 1269
GlaI GCGC 2 cut(s) 75, 537
GluI GCNGC 1 cut(s) 451
GsaI CCCAGC 1 cut(s) 1188
GsuI CTGGAG 1 cut(s) 945
HaeII RGCGCY 1 cut(s) 77
HaeIII GGCC 3 cut(s) 154, 291, 900
HapII CCGG 4 cut(s) 292, 348, 897, 1319
HgaI GACGC 2 cut(s) 699, 859
HhaI GCGC 2 cut(s) 76, 538
Hin1I GRCGYC 1 cut(s) 870
Hin1II CATG 7 cut(s) 701, 755, 784, 791, 1162, 1255, 1278
Hin6I GCGC 2 cut(s) 74, 536
HinP1I GCGC 2 cut(s) 74, 536
HincII GTYRAC 1 cut(s) 874
HindII GTYRAC 1 cut(s) 874
HinfI GANTC 7 cut(s) 145, 616, 740, 954, 968, 1143, 1200
HpaII CCGG 4 cut(s) 292, 348, 897, 1319
HphI GGTGA 3 cut(s) 496, 580, 1138
Hpy166II GTNNAC 3 cut(s) 857, 859, 874
Hpy188I TCNGA 4 cut(s) 621, 967, 1086, 1333
Hpy8I GTNNAC 3 cut(s) 857, 859, 874
Hpy99I CGWCG 1 cut(s) 482
HpyAV CCTTC 6 cut(s) 110, 130, 413, 417, 887, 1288
HpyCH4III ACNGT 4 cut(s) 31, 111, 161, 214
HpyCH4IV ACGT 2 cut(s) 480, 1239
HpyCH4V TGCA 8 cut(s) 67, 104, 666, 751, 759, 771, 986, 1067
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 707, 777, 936
HpyF3I CTNAG 1 cut(s) 270
HpySE526I ACGT 2 cut(s) 480, 1239
Hsp92I GRCGYC 1 cut(s) 870
Hsp92II CATG 7 cut(s) 701, 755, 784, 791, 1162, 1255, 1278
HspAI GCGC 2 cut(s) 74, 536
Kpn2I TCCGGA 1 cut(s) 1318
Ksp22I TGATCA 2 cut(s) 456, 1120
Kzo9I GATC 4 cut(s) 365, 456, 469, 1120
LguI GCTCTTC 1 cut(s) 341
LmnI GCTCC 2 cut(s) 306, 899
Lsp1109I GCAGC 1 cut(s) 462
LweI GCATC 2 cut(s) 31, 781
MaeI CTAG 7 cut(s) 11, 321, 723, 902, 944, 999, 1269
MaeII ACGT 2 cut(s) 480, 1239
MaeIII GTNAC 2 cut(s) 125, 1075
MalI GATC 4 cut(s) 367, 458, 471, 1122
MbiI CCGCTC 1 cut(s) 810
MboI GATC 4 cut(s) 365, 456, 469, 1120
MboII GAAGA 7 cut(s) 16, 38, 328, 686, 876, 1017, 1257
MflI RGATCY 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 271
MluCI AATT 8 cut(s) 390, 539, 559, 584, 1109, 1189, 1261, 1393
MlyI GAGTC 2 cut(s) 948, 962
MmeI TCCRAC 3 cut(s) 50, 169, 419
Mph1103I ATGCAT 1 cut(s) 753
MroI TCCGGA 1 cut(s) 1318
MroXI GAANNNNTTC 4 cut(s) 402, 1056, 1264, 1298
MseI TTAA 3 cut(s) 935, 1137, 1353
MslI CAYNNNNRTG 3 cut(s) 779, 864, 1101
MspI CCGG 4 cut(s) 292, 348, 897, 1319
MspR9I CCNGG 4 cut(s) 151, 288, 371, 435
Mva1269I GAATGC 1 cut(s) 232
MvaI CCWGG 4 cut(s) 151, 288, 371, 435
MvnI CGCG 2 cut(s) 420, 1287
MwoI GCNNNNNNNGC 4 cut(s) 73, 707, 777, 936
NdeII GATC 4 cut(s) 365, 456, 469, 1120
NlaIII CATG 7 cut(s) 701, 755, 784, 791, 1162, 1255, 1278
NlaIV GGNNCC 5 cut(s) 367, 399, 505, 895, 1323
NmuCI GTSAC 1 cut(s) 125
NruI TCGCGA 1 cut(s) 1287
NsiI ATGCAT 1 cut(s) 753
NspI RCATGY 1 cut(s) 755
OliI CACNNNNGTG 1 cut(s) 864
PagI TCATGA 1 cut(s) 787
PceI AGGCCT 1 cut(s) 154
PciSI GCTCTTC 1 cut(s) 341
PctI GAATGC 1 cut(s) 232
PdmI GAANNNNTTC 4 cut(s) 402, 1056, 1264, 1298
PfeI GAWTC 5 cut(s) 145, 616, 740, 1143, 1200
PflFI GACNNNGTC 1 cut(s) 1028
PinAI ACCGGT 1 cut(s) 347
PkrI GCNGC 1 cut(s) 452
PleI GAGTC 2 cut(s) 948, 962
PpsI GAGTC 2 cut(s) 948, 962
PsiI TTATAA 1 cut(s) 1392
Psp6I CCWGG 4 cut(s) 149, 286, 369, 433
PspFI CCCAGC 1 cut(s) 1184
PspGI CCWGG 4 cut(s) 149, 286, 369, 433
PspN4I GGNNCC 5 cut(s) 367, 399, 505, 895, 1323
PsuI RGATCY 1 cut(s) 365
PsyI GACNNNGTC 1 cut(s) 1028
RruI TCGCGA 1 cut(s) 1287
RsaI GTAC 2 cut(s) 858, 1402
RsaNI GTAC 2 cut(s) 857, 1401
RseI CAYNNNNRTG 3 cut(s) 779, 864, 1101
SapI GCTCTTC 1 cut(s) 341
SaqAI TTAA 3 cut(s) 935, 1137, 1353
SatI GCNGC 1 cut(s) 451
Sau3AI GATC 4 cut(s) 365, 456, 469, 1120
SchI GAGTC 2 cut(s) 948, 962
ScrFI CCNGG 4 cut(s) 151, 288, 371, 435
SduI GDGCHC 1 cut(s) 271
SfaNI GCATC 2 cut(s) 31, 781
SmiMI CAYNNNNRTG 3 cut(s) 779, 864, 1101
SmlI CTYRAG 3 cut(s) 803, 989, 1337
SmoI CTYRAG 3 cut(s) 803, 989, 1337
SpeI ACTAGT 1 cut(s) 722
Sse9I AATT 8 cut(s) 390, 539, 559, 584, 1109, 1189, 1261, 1393
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 3 cut(s) 284, 420, 810
SspI AATATT 1 cut(s) 1174
SspMI CTAG 7 cut(s) 11, 321, 723, 902, 944, 999, 1269
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 4 cut(s) 149, 286, 369, 433
TaaI ACNGT 4 cut(s) 31, 111, 161, 214
TaiI ACGT 2 cut(s) 483, 1242
TaqI TCGA 1 cut(s) 614
TasI AATT 8 cut(s) 390, 539, 559, 584, 1109, 1189, 1261, 1393
TatI WGTACW 1 cut(s) 856
TfiI GAWTC 5 cut(s) 145, 616, 740, 1143, 1200
Tru1I TTAA 3 cut(s) 935, 1137, 1353
Tru9I TTAA 3 cut(s) 935, 1137, 1353
TscAI CASTG 4 cut(s) 166, 307, 389, 1069
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 1 cut(s) 450
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 7 cut(s) 17, 129, 328, 641, 686, 964, 1034
TspGWI ACGGA 1 cut(s) 477
TspRI CASTG 4 cut(s) 166, 307, 389, 1069
Tth111I GACNNNGTC 1 cut(s) 1028
XapI RAATTY 4 cut(s) 584, 1109, 1189, 1261
XceI RCATGY 1 cut(s) 755
XmnI GAANNNNTTC 4 cut(s) 402, 1056, 1264, 1298
XspI CTAG 7 cut(s) 11, 321, 723, 902, 944, 999, 1269
Zsp2I ATGCAT 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.