RchiOBHm_Chr6g0294531

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005900
Physical Location & Seq
Forward (+)
56798106 .. 56800225
2120 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26434

Sequence Viewer

Length: 1458 bp
ATGAAGAGGCTAAGGACGTTTGAGTTTTGTGTGCTGATTGATTGGATATGGTGTGCAACAGGTAGGCATCCAACTGTGGTTCAACCTTTTCTTGTTGTTTTTTCTTATTTTCAGTTTGCGCTGGTTTTGGTTCTGGGATTTACTAGTGCATTACAGTACGATGGAGAATGTGAAAACAAGAAGGCATTGAATCCCAGGCCACACAGTGTGTCGCTTTTGGAGTTTGGGGCAGTCGGAGATGGGAAAACATTGAACACAGTAGCTTTCCAAAATGCCGTTTTCTATGTCAAGTCATTTGCTGACAAGGGTGGTGCTCAGCTCTATGTTCCGCCTGGCCGGTGGCTCACTGGGAGCTTTAACCTCACCAGCCACCTCACGCTCTTCCTTGAGACCGGTGCAACTATTCTTGGATCCCAGGACTATACTCACTGGGAAATTGAGGAACCCCTTCCTTCTTATGGTCGCGGGATTGATTTTCCAGGTTGGAGATATCGTAGCTTGATCAACGGTGATGATCTACACGATGTGGTGATAACAGGTGGTAATGGAACCATAGATGGACAGGGTTCTGTGTGGTGGGAGCAATTCAGCTCTCATTCGTTGAATTACAGCAGACCACATCTTGTGGAATTCACCAGATGTGATCATATCGTTATATCGAATCTGACTTTCTTGAACTCCCCGGCTTGGAACATTCATCCTACATATTGCAGGTATGTGAAAGTCCAAAACTTAACTGTTCATGCTTCTCCTGCATCTCCATATACTAGCGGAATAGTCCCAGATTCTTCTGAGCATGTTTGCATTGAGAACTGCAACATTAGTATGGGTCATGATGCAATTGTTCTTAAGAGCGGTTGGGATGAGTATGGAATCGCCTATGGCAAACCGACCAAAAGTGTACACATCCGGGCCGTCAACCTTCAATCTTCTTTAGGCTCCGGCCTGGCATTTGGGAGTGAGATGTCTGGAGGCATTTTAAACGTACTAGTAGAGCGGCTTCATCTGTCTAACTCATTCGTTGGCATTGAACTCAAGACAACCAGAGGAAGAGGAGGATATATGAAAGACATTGTTATATCGAATGTAGAAATGCACAACATTCACACAGCAATCCAGGTCACTGGTCAGAGTGGCTCTCATCCTGATGATAAGTTTGACCCTGATCAAGTTCCATTTGTTAAAGGAATCACCTTTGACAACATGGTCGGCAGTAATATTACATTTGCTGGGAATTTTTCTGGAATCTACGAATCACCTTTCACATCTATTTGTCTCTCAAATGTGTCATTCTCCATGAATTGGGATTCTTCTAGCTCATGGTTCTGTTCCGATGTGAAGGGGTTTTCCCAGAAGGTTTCTCCAGAACCATGTTCAGACCTCAAGGAATCATCTTTTAATGCTTCCTCATCTTGCTTCTTTTCCTTATACCCTTACAATTATGCTACAGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

485

Amino Acids

53.68

Weight (kDa)

5.76

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 166 - 447 1.3e-31 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1205
Acc36I ACCTGC 1 cut(s) 702
AccB7I CCANNNNNTGG 1 cut(s) 1302
AccBSI CCGCTC 2 cut(s) 855, 997
AccII CGCG 1 cut(s) 465
AciI CCGC 5 cut(s) 329, 465, 771, 855, 997
AclWI GGATC 2 cut(s) 405, 418
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 2 cut(s) 629, 1234
AdeI CACNNNGTG 3 cut(s) 206, 208, 526
AfaI GTAC 3 cut(s) 158, 903, 987
AfiI CCNNNNNNNGG 3 cut(s) 458, 687, 1302
AflII CTTAAG 1 cut(s) 848
AgeI ACCGGT 1 cut(s) 392
AgsI TTSAA 7 cut(s) 83, 190, 253, 604, 676, 926, 1031
AhlI ACTAGT 2 cut(s) 143, 988
AjnI CCWGG 6 cut(s) 194, 331, 414, 478, 945, 1116
AluBI AGCT 6 cut(s) 263, 319, 354, 498, 591, 1317
AluI AGCT 6 cut(s) 263, 319, 354, 498, 591, 1317
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 2 cut(s) 383, 1280
AlwI GGATC 2 cut(s) 405, 418
AoxI GGCC 4 cut(s) 197, 334, 912, 943
ApoI RAATTY 2 cut(s) 629, 1234
AsiGI ACCGGT 1 cut(s) 392
Asp700I GAANNNNTTC 1 cut(s) 447
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 1 cut(s) 912
AsuC2I CCSGG 2 cut(s) 683, 911
AsuHPI GGTGA 6 cut(s) 355, 521, 541, 625, 1183, 1248
BamHI GGATCC 1 cut(s) 410
BarI GAAGNNNNNNTAC 2 cut(s) 984, 1016
Bbv12I GWGCWC 1 cut(s) 316
BccI CCATC 3 cut(s) 155, 233, 551
BceAI ACGGC 2 cut(s) 260, 899
BciT130I CCWGG 6 cut(s) 196, 333, 416, 480, 947, 1118
BclI TGATCA 3 cut(s) 501, 643, 1165
BcnI CCSGG 2 cut(s) 683, 911
BcoDI GTCTC 2 cut(s) 383, 1280
BcuI ACTAGT 2 cut(s) 143, 988
BfaI CTAG 4 cut(s) 144, 768, 989, 1314
BfmI CTRYAG 1 cut(s) 1446
BfrI CTTAAG 1 cut(s) 848
BfuAI ACCTGC 1 cut(s) 702
BisI GCNGC 1 cut(s) 998
BlpI GCTNAGC 1 cut(s) 315
BlsI GCNGC 1 cut(s) 999
Bme1390I CCNGG 8 cut(s) 196, 333, 416, 480, 683, 911, 947, 1118
BmgT120I GGNCC 1 cut(s) 912
BmiI GGNNCC 4 cut(s) 412, 444, 550, 940
BmrFI CCNGG 8 cut(s) 196, 333, 416, 480, 683, 911, 947, 1118
BmrI ACTGGG 2 cut(s) 357, 439
BmsI GCATC 3 cut(s) 76, 764, 826
BmuI ACTGGG 2 cut(s) 357, 439
BplI GAGNNNNNCTC 2 cut(s) 1123, 1155
BpmI CTGGAG 2 cut(s) 990, 1347
Bpu10I CCTNAGC 1 cut(s) 11
Bpu1102I GCTNAGC 1 cut(s) 315
BpuEI CTTGAG 3 cut(s) 407, 1019, 1367
BpuMI CCSGG 2 cut(s) 683, 911
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 3 cut(s) 194, 414, 681
BsaWI WCCGGW 1 cut(s) 392
BsaXI ACNNNNNCTCC 2 cut(s) 478, 508
Bsc4I CCNNNNNNNGG 3 cut(s) 458, 687, 1302
Bse118I RCCGGY 2 cut(s) 336, 392
Bse1I ACTGG 3 cut(s) 352, 434, 1129
BseBI CCWGG 6 cut(s) 196, 333, 416, 480, 947, 1118
BseDI CCNNGG 3 cut(s) 194, 414, 681
BseGI GGATG 5 cut(s) 67, 697, 868, 906, 1141
BseLI CCNNNNNNNGG 3 cut(s) 458, 687, 1302
BseMII CTCAG 2 cut(s) 329, 783
BseNI ACTGG 3 cut(s) 352, 434, 1129
BseRI GAGGAG 1 cut(s) 1068
BseYI CCCAGC 1 cut(s) 1229
Bsh1236I CGCG 1 cut(s) 465
BshFI GGCC 4 cut(s) 199, 336, 914, 945
BshTI ACCGGT 1 cut(s) 392
BsiHKAI GWGCWC 1 cut(s) 316
BsiSI CCGG 5 cut(s) 337, 393, 683, 910, 942
BslFI GGGAC 1 cut(s) 764
BslI CCNNNNNNNGG 3 cut(s) 458, 687, 1302
BsmAI GTCTC 2 cut(s) 383, 1280
BsmFI GGGAC 1 cut(s) 764
BsnI GGCC 4 cut(s) 199, 336, 914, 945
Bso31I GGTCTC 1 cut(s) 383
Bsp1286I GDGCHC 1 cut(s) 316
Bsp1407I TGTACA 1 cut(s) 901
Bsp143I GATC 5 cut(s) 410, 501, 514, 643, 1165
Bsp1720I GCTNAGC 1 cut(s) 315
BspACI CCGC 5 cut(s) 329, 465, 771, 855, 997
BspANI GGCC 4 cut(s) 199, 336, 914, 945
BspCNI CTCAG 2 cut(s) 328, 784
BspFNI CGCG 1 cut(s) 465
BspHI TCATGA 1 cut(s) 832
BspLI GGNNCC 4 cut(s) 412, 444, 550, 940
BspMI ACCTGC 1 cut(s) 702
BspPI GGATC 2 cut(s) 405, 418
BspQI GCTCTTC 1 cut(s) 386
BspTI CTTAAG 1 cut(s) 848
BspTNI GGTCTC 1 cut(s) 383
BsrBI CCGCTC 2 cut(s) 855, 997
BsrFI RCCGGY 2 cut(s) 336, 392
BsrGI TGTACA 1 cut(s) 901
BsrI ACTGG 3 cut(s) 352, 434, 1129
BssAI RCCGGY 2 cut(s) 336, 392
BssECI CCNNGG 3 cut(s) 194, 414, 681
BssMI GATC 5 cut(s) 410, 501, 514, 643, 1165
Bst2UI CCWGG 6 cut(s) 196, 333, 416, 480, 947, 1118
Bst4CI ACNGT 6 cut(s) 76, 156, 206, 259, 509, 739
Bst6I CTCTTC 2 cut(s) 386, 1045
BstAFI CTTAAG 1 cut(s) 848
BstAUI TGTACA 1 cut(s) 901
BstDEI CTNAG 3 cut(s) 11, 315, 792
BstF5I GGATG 5 cut(s) 67, 697, 868, 906, 1141
BstFNI CGCG 1 cut(s) 465
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 5 cut(s) 413, 504, 517, 646, 1168
BstMAI GTCTC 2 cut(s) 383, 1280
BstMBI GATC 5 cut(s) 410, 501, 514, 643, 1165
BstMWI GCNNNNNNNGC 1 cut(s) 752
BstNI CCWGG 6 cut(s) 196, 333, 416, 480, 947, 1118
BstNSI RCATGY 1 cut(s) 800
BstSCI CCNGG 8 cut(s) 194, 331, 414, 478, 681, 909, 945, 1116
BstSFI CTRYAG 1 cut(s) 1446
BstUI CGCG 1 cut(s) 465
BstX2I RGATCY 1 cut(s) 410
BstXI CCANNNNNNTGG 1 cut(s) 1124
BstYI RGATCY 1 cut(s) 410
BsuRI GGCC 4 cut(s) 199, 336, 914, 945
BtsCI GGATG 5 cut(s) 67, 697, 868, 906, 1141
BtsIMutI CAGTG 4 cut(s) 211, 345, 427, 1122
BveI ACCTGC 1 cut(s) 702
CciI TCATGA 1 cut(s) 832
CfoI GCGC 1 cut(s) 121
Cfr10I RCCGGY 2 cut(s) 336, 392
Cfr13I GGNCC 1 cut(s) 912
Csp6I GTAC 3 cut(s) 157, 902, 986
CspAI ACCGGT 1 cut(s) 392
CviAII CATG 7 cut(s) 743, 797, 833, 1204, 1297, 1320, 1371
CviQI GTAC 3 cut(s) 157, 902, 986
DdeI CTNAG 3 cut(s) 11, 315, 792
DpnI GATC 5 cut(s) 412, 503, 516, 645, 1167
DpnII GATC 5 cut(s) 410, 501, 514, 643, 1165
DraI TTTAAA 1 cut(s) 981
DraIII CACNNNGTG 3 cut(s) 206, 208, 526
DrdI GACNNNNNNGTC 1 cut(s) 1205
DseDI GACNNNNNNGTC 1 cut(s) 1205
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 2 cut(s) 386, 1045
EarI CTCTTC 2 cut(s) 386, 1045
EciI GGCGGA 1 cut(s) 318
Eco31I GGTCTC 1 cut(s) 383
Eco32I GATATC 1 cut(s) 491
EcoRI GAATTC 1 cut(s) 629
EcoRII CCWGG 6 cut(s) 194, 331, 414, 478, 945, 1116
EcoRV GATATC 1 cut(s) 491
FaeI CATG 7 cut(s) 746, 800, 836, 1207, 1300, 1323, 1374
FaqI GGGAC 1 cut(s) 764
FatI CATG 7 cut(s) 742, 796, 832, 1203, 1296, 1319, 1370
FauI CCCGC 1 cut(s) 458
FbaI TGATCA 3 cut(s) 501, 643, 1165
Fnu4HI GCNGC 1 cut(s) 998
FokI GGATG 5 cut(s) 54, 684, 875, 893, 1128
Fsp4HI GCNGC 1 cut(s) 998
FspBI CTAG 4 cut(s) 144, 768, 989, 1314
GlaI GCGC 1 cut(s) 120
GluI GCNGC 1 cut(s) 998
GsaI CCCAGC 1 cut(s) 1233
GsuI CTGGAG 2 cut(s) 990, 1347
HaeIII GGCC 4 cut(s) 199, 336, 914, 945
HapII CCGG 5 cut(s) 337, 393, 683, 910, 942
HhaI GCGC 1 cut(s) 121
Hin1II CATG 7 cut(s) 746, 800, 836, 1207, 1300, 1323, 1374
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HincII GTYRAC 1 cut(s) 919
HindII GTYRAC 1 cut(s) 919
HinfI GANTC 9 cut(s) 190, 661, 785, 873, 1188, 1245, 1253, 1307, 1388
HpaII CCGG 5 cut(s) 337, 393, 683, 910, 942
HphI GGTGA 6 cut(s) 355, 521, 541, 625, 1183, 1248
Hpy166II GTNNAC 3 cut(s) 902, 904, 919
Hpy188I TCNGA 6 cut(s) 236, 666, 793, 1131, 1333, 1378
Hpy188III TCNNGA 7 cut(s) 673, 833, 969, 1036, 1145, 1242, 1364
Hpy8I GTNNAC 3 cut(s) 902, 904, 919
HpyAV CCTTC 6 cut(s) 175, 458, 462, 932, 1333, 1348
HpyCH4III ACNGT 6 cut(s) 76, 156, 206, 259, 509, 739
HpyCH4IV ACGT 2 cut(s) 17, 984
HpyCH4V TGCA 9 cut(s) 56, 149, 398, 711, 755, 804, 816, 839, 1096
HpyF10VI GCNNNNNNNGC 1 cut(s) 752
HpyF3I CTNAG 3 cut(s) 11, 315, 792
HpySE526I ACGT 2 cut(s) 17, 984
Hsp92II CATG 7 cut(s) 746, 800, 836, 1207, 1300, 1323, 1374
HspAI GCGC 1 cut(s) 119
Ksp22I TGATCA 3 cut(s) 501, 643, 1165
Kzo9I GATC 5 cut(s) 410, 501, 514, 643, 1165
LguI GCTCTTC 1 cut(s) 386
LmnI GCTCC 3 cut(s) 351, 580, 944
LweI GCATC 3 cut(s) 76, 764, 826
MaeI CTAG 4 cut(s) 144, 768, 989, 1314
MaeII ACGT 2 cut(s) 17, 984
MaeIII GTNAC 1 cut(s) 1120
MalI GATC 5 cut(s) 412, 503, 516, 645, 1167
MbiI CCGCTC 2 cut(s) 855, 997
MboI GATC 5 cut(s) 410, 501, 514, 643, 1165
MboII GAAGA 6 cut(s) 16, 373, 780, 921, 1062, 1302
MfeI CAATTG 1 cut(s) 840
MflI RGATCY 1 cut(s) 410
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 8 cut(s) 435, 584, 604, 629, 840, 1234, 1300, 1438
MmeI TCCRAC 3 cut(s) 95, 214, 464
MnlI CCTC 9 cut(s) 371, 383, 433, 965, 1040, 1046, 1049, 1391, 1417
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 6 cut(s) 357, 734, 849, 980, 1182, 1398
MslI CAYNNNNRTG 2 cut(s) 824, 1146
MspCI CTTAAG 1 cut(s) 848
MspI CCGG 5 cut(s) 337, 393, 683, 910, 942
MspR9I CCNGG 8 cut(s) 196, 333, 416, 480, 683, 911, 947, 1118
MunI CAATTG 1 cut(s) 840
MvaI CCWGG 6 cut(s) 196, 333, 416, 480, 947, 1118
MvnI CGCG 1 cut(s) 465
MwoI GCNNNNNNNGC 1 cut(s) 752
NciI CCSGG 2 cut(s) 683, 911
NdeII GATC 5 cut(s) 410, 501, 514, 643, 1165
NlaIII CATG 7 cut(s) 746, 800, 836, 1207, 1300, 1323, 1374
NlaIV GGNNCC 4 cut(s) 412, 444, 550, 940
NmuCI GTSAC 1 cut(s) 1120
NspI RCATGY 1 cut(s) 800
PagI TCATGA 1 cut(s) 832
PciSI GCTCTTC 1 cut(s) 386
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 9 cut(s) 190, 661, 785, 873, 1188, 1245, 1253, 1307, 1388
PflMI CCANNNNNTGG 1 cut(s) 1302
PinAI ACCGGT 1 cut(s) 392
PkrI GCNGC 1 cut(s) 999
Psp6I CCWGG 6 cut(s) 194, 331, 414, 478, 945, 1116
PspFI CCCAGC 1 cut(s) 1229
PspGI CCWGG 6 cut(s) 194, 331, 414, 478, 945, 1116
PspN4I GGNNCC 4 cut(s) 412, 444, 550, 940
PspPI GGNCC 1 cut(s) 912
PsuI RGATCY 1 cut(s) 410
RsaI GTAC 3 cut(s) 158, 903, 987
RsaNI GTAC 3 cut(s) 157, 902, 986
RseI CAYNNNNRTG 2 cut(s) 824, 1146
SapI GCTCTTC 1 cut(s) 386
SaqAI TTAA 6 cut(s) 357, 734, 849, 980, 1182, 1398
SatI GCNGC 1 cut(s) 998
Sau3AI GATC 5 cut(s) 410, 501, 514, 643, 1165
Sau96I GGNCC 1 cut(s) 912
ScrFI CCNGG 8 cut(s) 196, 333, 416, 480, 683, 911, 947, 1118
SduI GDGCHC 1 cut(s) 316
SfaNI GCATC 3 cut(s) 76, 764, 826
SfcI CTRYAG 1 cut(s) 1446
SmiMI CAYNNNNRTG 2 cut(s) 824, 1146
SmlI CTYRAG 4 cut(s) 386, 848, 1034, 1382
SmoI CTYRAG 4 cut(s) 386, 848, 1034, 1382
SpeI ACTAGT 2 cut(s) 143, 988
Sse9I AATT 8 cut(s) 435, 584, 604, 629, 840, 1234, 1300, 1438
SsiI CCGC 5 cut(s) 329, 465, 771, 855, 997
SspI AATATT 1 cut(s) 1219
SspMI CTAG 4 cut(s) 144, 768, 989, 1314
StyD4I CCNGG 8 cut(s) 194, 331, 414, 478, 681, 909, 945, 1116
TaaI ACNGT 6 cut(s) 76, 156, 206, 259, 509, 739
TaiI ACGT 2 cut(s) 20, 987
TaqI TCGA 2 cut(s) 659, 1082
TasI AATT 8 cut(s) 435, 584, 604, 629, 840, 1234, 1300, 1438
TatI WGTACW 1 cut(s) 901
TauI GCSGC 1 cut(s) 1000
TfiI GAWTC 9 cut(s) 190, 661, 785, 873, 1188, 1245, 1253, 1307, 1388
Tru1I TTAA 6 cut(s) 357, 734, 849, 980, 1182, 1398
Tru9I TTAA 6 cut(s) 357, 734, 849, 980, 1182, 1398
TscAI CASTG 4 cut(s) 211, 352, 434, 1129
TseFI GTSAC 1 cut(s) 1120
Tsp45I GTSAC 1 cut(s) 1120
TspDTI ATGAA 6 cut(s) 17, 686, 731, 992, 1079, 1313
TspRI CASTG 4 cut(s) 211, 352, 434, 1129
Van91I CCANNNNNTGG 1 cut(s) 1302
Vha464I CTTAAG 1 cut(s) 848
XapI RAATTY 2 cut(s) 629, 1234
XceI RCATGY 1 cut(s) 800
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 4 cut(s) 144, 768, 989, 1314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.