MD03G1292400.v1.1

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005900
Physical Location & Seq
Forward (+)
36939589 .. 36943139
3551 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1292400.v1.1.491

Sequence Viewer

Length: 1362 bp
ATGAAGAGCCTACTGGCCCTGGTTTTGATTCTGGCATTTACTAGTGCCGAGTATGATGGAGAAGGAAAGGACAGAGAATGCGAAGGGAAGAAGCTTTTCGATCCCAGACCTCACAGTGTGTCACTCTTGGAGTTTGGGGCAGTTGGAGACGGGAAAACATTAAACACAGTCGCCTTCCAAAACGCTATCTTCTATGTCAAGTCATTTGCTGACAAGGGTGGTGCTCAGCTCTATGTTCCACCCGGCAGGTGGCTCACCGGGAGTTTTAACCTCACCAGCCACCTCACGCTCTTTTTGGAAGCCGGTGCCATCATTCTTGGATCCCAGGATTATGCTCACTGGGAAATTGTGGAACCCCTGCCTTCTTATGGTCGAGGGATTGACTTTCCGGGTTGGAGATATCGTAGCCTGATCAACGGAGACAATCTAACCGATGTGATGATAACAGGCGATAATGGAACCATTGATGGGCAGGGTTTCGTCTGGTGGGAGCAATTCAGCTATCATGCATTGAATTACAGCCGACCACATCTAGTCGAATTCACCAGATGCGAAAATGTCACTGTATCGAATCTAACCCTCTTGAACTCCCCTGCTTGGCACATTCATCCGACATACTGCAGGAATGTGCAAGTTCTGAACATAACTCTCCATGCTTCTCCTGCATCTCCATTTACTAGCGGTGTAGTCCCAGATTCTTCCGAACATGTTTGCATTGAGAACTGTAACATTAGTATGGGGCATGACGCAATTGTTCTCAAGAGCGGTTGGGATGAGTATGGAATTGCCTTTGGAAAACCAACCACAAATGTGCACATCCGTGGCGTTAACCTTCAGTCTTCTTTAGGCTCCGGCCTGGCATTTGGCAGCGAGATGTCTGGAGGCATATCAAATGTTCGTGTAGAGCAGCTTCGTCTACATGACTCATTCATCGGCATTGCACTCAAGACAGCTATGGGAAGAGGAGGTTATATGAAAGACATAGTTATATCCAATGTCGAAATGGAGAACATTCGCTTGGCAATTGAGGCTACTGGTCAGATCGGTTTGCATCCTGATGAAAAATATGATCCCGATGAACTACCAGTTGTTAAAGGTATCACCTTTGAGAATATGGTTGGCACTAATGTTACATTAGCTGGAAACTTTTCTGGACTCGAAAAATCGCCTTTCACTTCCATCTGTCTCTCAAATATCTCATTCGCCCTCACTTGGAATTCTTCTACCTCGTGGTTCTGTTCCAATGTGATCGGTTTTTCACAGGAAGTTTCTCCCAAACCGTGTTCAAACCTCCGGGAATCGGCTTTTAACTCTTCCTCAGCTTGCTTCTTTCCGTTATATAATTATGCTTCAGTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

454

Amino Acids

49.57

Weight (kDa)

5.37

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 136 - 416 8e-33 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 237
Acc36I ACCTGC 1 cut(s) 237
AccB1I GGYRCC 1 cut(s) 305
AccBSI CCGCTC 1 cut(s) 765
AccI GTMKAC 1 cut(s) 916
AciI CCGC 2 cut(s) 681, 765
AclWI GGATC 4 cut(s) 95, 315, 328, 1064
AcsI RAATTY 2 cut(s) 539, 1216
AcuI CTGAAG 2 cut(s) 818, 1335
AdeI CACNNNGTG 1 cut(s) 118
AfiI CCNNNNNNNGG 6 cut(s) 249, 368, 468, 597, 1212, 1300
AflIII ACRYGT 1 cut(s) 706
AgsI TTSAA 3 cut(s) 514, 586, 1287
AhlI ACTAGT 1 cut(s) 41
AjnI CCWGG 3 cut(s) 18, 324, 855
AjuI GAANNNNNNNTTGG 2 cut(s) 1001, 1033
AleI CACNNNNGTG 2 cut(s) 809, 819
AluBI AGCT 7 cut(s) 94, 229, 501, 910, 953, 1139, 1322
AluI AGCT 7 cut(s) 94, 229, 501, 910, 953, 1139, 1322
Alw21I GWGCWC 2 cut(s) 226, 816
Alw26I GTCTC 3 cut(s) 141, 414, 1190
Alw44I GTGCAC 1 cut(s) 812
AlwI GGATC 4 cut(s) 95, 315, 328, 1064
AoxI GGCC 2 cut(s) 15, 853
ApaLI GTGCAC 1 cut(s) 812
ApeKI GCWGC 2 cut(s) 867, 907
ApoI RAATTY 2 cut(s) 539, 1216
Asp700I GAANNNNTTC 2 cut(s) 95, 1147
AspS9I GGNCC 1 cut(s) 16
AsuC2I CCSGG 4 cut(s) 243, 259, 390, 1295
AsuHPI GGTGA 4 cut(s) 247, 265, 535, 1093
BaeGI GKGCMC 1 cut(s) 816
BamHI GGATCC 1 cut(s) 320
BanI GGYRCC 1 cut(s) 305
BarI GAAGNNNNNNTAC 2 cut(s) 894, 926
BauI CACGAG 1 cut(s) 1228
BbsI GAAGAC 1 cut(s) 831
Bbv12I GWGCWC 2 cut(s) 226, 816
BbvCI CCTCAGC 1 cut(s) 1318
BbvI GCAGC 2 cut(s) 879, 919
BccI CCATC 4 cut(s) 50, 317, 461, 1187
BciT130I CCWGG 3 cut(s) 20, 326, 857
BclI TGATCA 1 cut(s) 411
BcnI CCSGG 4 cut(s) 243, 259, 390, 1295
BcoDI GTCTC 3 cut(s) 141, 414, 1190
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 3 cut(s) 42, 533, 678
BfmI CTRYAG 1 cut(s) 619
BfuAI ACCTGC 1 cut(s) 237
BisI GCNGC 2 cut(s) 868, 908
BlpI GCTNAGC 1 cut(s) 225
BlsI GCNGC 2 cut(s) 869, 909
Bme1390I CCNGG 7 cut(s) 20, 243, 259, 326, 390, 857, 1295
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 5 cut(s) 307, 322, 354, 460, 850
BmrFI CCNGG 7 cut(s) 20, 243, 259, 326, 390, 857, 1295
BmrI ACTGGG 1 cut(s) 349
BmsI GCATC 3 cut(s) 539, 674, 1060
BmuI ACTGGG 1 cut(s) 349
BpiI GAAGAC 1 cut(s) 831
BpmI CTGGAG 1 cut(s) 900
Bpu10I CCTNAGC 1 cut(s) 1318
Bpu1102I GCTNAGC 1 cut(s) 225
BpuEI CTTGAG 2 cut(s) 743, 929
BpuMI CCSGG 4 cut(s) 243, 259, 390, 1295
BsaJI CCNNGG 3 cut(s) 18, 324, 820
BsaXI ACNNNNNCTCC 2 cut(s) 388, 418
Bsc4I CCNNNNNNNGG 6 cut(s) 249, 368, 468, 597, 1212, 1300
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 4 cut(s) 18, 344, 1039, 1085
Bse3DI GCAATG 1 cut(s) 936
BseBI CCWGG 3 cut(s) 20, 326, 857
BseDI CCNNGG 3 cut(s) 18, 324, 820
BseGI GGATG 4 cut(s) 607, 778, 816, 1051
BseLI CCNNNNNNNGG 6 cut(s) 249, 368, 468, 597, 1212, 1300
BseMI GCAATG 1 cut(s) 936
BseMII CTCAG 2 cut(s) 239, 1332
BseNI ACTGG 4 cut(s) 18, 344, 1039, 1085
BseRI GAGGAG 1 cut(s) 978
BseSI GKGCMC 1 cut(s) 816
BseXI GCAGC 2 cut(s) 879, 919
BshFI GGCC 2 cut(s) 17, 855
BshNI GGYRCC 1 cut(s) 305
BsiHKAI GWGCWC 2 cut(s) 226, 816
BsiSI CCGG 6 cut(s) 243, 258, 303, 389, 852, 1294
BslFI GGGAC 1 cut(s) 674
BslI CCNNNNNNNGG 6 cut(s) 249, 368, 468, 597, 1212, 1300
BsmAI GTCTC 3 cut(s) 141, 414, 1190
BsmBI CGTCTC 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 674
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 2 cut(s) 17, 855
Bsp1286I GDGCHC 2 cut(s) 226, 816
Bsp143I GATC 6 cut(s) 100, 320, 411, 1041, 1069, 1248
Bsp1720I GCTNAGC 1 cut(s) 225
BspACI CCGC 2 cut(s) 681, 765
BspANI GGCC 2 cut(s) 17, 855
BspCNI CTCAG 2 cut(s) 238, 1331
BspLI GGNNCC 5 cut(s) 307, 322, 354, 460, 850
BspMAI CTGCAG 1 cut(s) 623
BspMI ACCTGC 1 cut(s) 237
BspPI GGATC 4 cut(s) 95, 315, 328, 1064
BspT107I GGYRCC 1 cut(s) 305
BsrBI CCGCTC 1 cut(s) 765
BsrDI GCAATG 1 cut(s) 936
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 4 cut(s) 18, 344, 1039, 1085
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 3 cut(s) 18, 324, 820
BssMI GATC 6 cut(s) 100, 320, 411, 1041, 1069, 1248
BssSI CACGAG 1 cut(s) 1228
Bst2BI CACGAG 1 cut(s) 1228
Bst2UI CCWGG 3 cut(s) 20, 326, 857
Bst4CI ACNGT 5 cut(s) 116, 169, 565, 725, 1281
Bst6I CTCTTC 2 cut(s) 955, 1318
BstC8I GCNNGC 1 cut(s) 1324
BstDEI CTNAG 2 cut(s) 225, 1318
BstDSI CCRYGG 1 cut(s) 820
BstF5I GGATG 4 cut(s) 607, 778, 816, 1051
BstKTI GATC 6 cut(s) 103, 323, 414, 1044, 1072, 1251
BstMAI GTCTC 3 cut(s) 141, 414, 1190
BstMBI GATC 6 cut(s) 100, 320, 411, 1041, 1069, 1248
BstMWI GCNNNNNNNGC 2 cut(s) 662, 1028
BstNI CCWGG 3 cut(s) 20, 326, 857
BstNSI RCATGY 1 cut(s) 710
BstSCI CCNGG 7 cut(s) 18, 241, 257, 324, 388, 855, 1293
BstSFI CTRYAG 1 cut(s) 619
BstSLI GKGCMC 1 cut(s) 816
BstV1I GCAGC 2 cut(s) 879, 919
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 1 cut(s) 320
BstYI RGATCY 1 cut(s) 320
BsuRI GGCC 2 cut(s) 17, 855
BtgI CCRYGG 1 cut(s) 820
BtsCI GGATG 4 cut(s) 607, 778, 816, 1051
BtsIMutI CAGTG 3 cut(s) 121, 337, 561
BveI ACCTGC 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 1324
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 16
CseI GACGC 1 cut(s) 755
CviAII CATG 5 cut(s) 506, 653, 707, 743, 920
DdeI CTNAG 2 cut(s) 225, 1318
DpnI GATC 6 cut(s) 102, 322, 413, 1043, 1071, 1250
DpnII GATC 6 cut(s) 100, 320, 411, 1041, 1069, 1248
DraIII CACNNNGTG 1 cut(s) 118
Eam1104I CTCTTC 2 cut(s) 955, 1318
EarI CTCTTC 2 cut(s) 955, 1318
Eco32I GATATC 1 cut(s) 401
Eco57I CTGAAG 2 cut(s) 818, 1335
EcoRI GAATTC 2 cut(s) 539, 1216
EcoRII CCWGG 3 cut(s) 18, 324, 855
EcoRV GATATC 1 cut(s) 401
EcoT22I ATGCAT 1 cut(s) 511
Esp3I CGTCTC 1 cut(s) 141
FaeI CATG 5 cut(s) 509, 656, 710, 746, 923
FaqI GGGAC 1 cut(s) 674
FatI CATG 5 cut(s) 505, 652, 706, 742, 919
FbaI TGATCA 1 cut(s) 411
FblI GTMKAC 1 cut(s) 916
Fnu4HI GCNGC 2 cut(s) 868, 908
FokI GGATG 4 cut(s) 594, 785, 803, 1038
Fsp4HI GCNGC 2 cut(s) 868, 908
FspBI CTAG 3 cut(s) 42, 533, 678
GluI GCNGC 2 cut(s) 868, 908
GsuI CTGGAG 1 cut(s) 900
HaeIII GGCC 2 cut(s) 17, 855
HapII CCGG 6 cut(s) 243, 258, 303, 389, 852, 1294
HgaI GACGC 1 cut(s) 755
Hin1II CATG 5 cut(s) 509, 656, 710, 746, 923
HincII GTYRAC 1 cut(s) 829
HindII GTYRAC 1 cut(s) 829
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 6 cut(s) 28, 571, 695, 923, 1155, 1298
HpaI GTTAAC 1 cut(s) 829
HpaII CCGG 6 cut(s) 243, 258, 303, 389, 852, 1294
HphI GGTGA 4 cut(s) 247, 265, 535, 1093
Hpy166II GTNNAC 3 cut(s) 814, 829, 917
Hpy188I TCNGA 4 cut(s) 612, 639, 703, 1041
Hpy188III TCNNGA 7 cut(s) 583, 760, 879, 946, 1055, 1073, 1152
Hpy8I GTNNAC 3 cut(s) 814, 829, 917
HpyAV CCTTC 5 cut(s) 56, 77, 184, 372, 842
HpyCH4III ACNGT 5 cut(s) 116, 169, 565, 725, 1281
HpyCH4V TGCA 8 cut(s) 509, 621, 631, 665, 714, 814, 941, 1051
HpyF10VI GCNNNNNNNGC 2 cut(s) 662, 1028
HpyF3I CTNAG 2 cut(s) 225, 1318
Hsp92II CATG 5 cut(s) 509, 656, 710, 746, 923
Ksp22I TGATCA 1 cut(s) 411
KspAI GTTAAC 1 cut(s) 829
Kzo9I GATC 6 cut(s) 100, 320, 411, 1041, 1069, 1248
LmnI GCTCC 2 cut(s) 490, 854
Lsp1109I GCAGC 2 cut(s) 879, 919
LweI GCATC 3 cut(s) 539, 674, 1060
MaeI CTAG 3 cut(s) 42, 533, 678
MaeIII GTNAC 4 cut(s) 120, 559, 725, 1129
MalI GATC 6 cut(s) 102, 322, 413, 1043, 1071, 1250
MbiI CCGCTC 1 cut(s) 765
MboI GATC 6 cut(s) 100, 320, 411, 1041, 1069, 1248
MboII GAAGA 8 cut(s) 16, 100, 181, 690, 831, 972, 1212, 1305
MfeI CAATTG 2 cut(s) 750, 1023
MflI RGATCY 1 cut(s) 320
MhlI GDGCHC 2 cut(s) 226, 816
MluCI AATT 9 cut(s) 345, 494, 514, 539, 750, 783, 1023, 1216, 1342
MlyI GAGTC 2 cut(s) 917, 1149
MmeI TCCRAC 3 cut(s) 124, 374, 635
Mph1103I ATGCAT 1 cut(s) 511
MroXI GAANNNNTTC 2 cut(s) 95, 1147
MseI TTAA 5 cut(s) 161, 267, 828, 1092, 1308
MslI CAYNNNNRTG 4 cut(s) 734, 809, 819, 1056
MspI CCGG 6 cut(s) 243, 258, 303, 389, 852, 1294
MspR9I CCNGG 7 cut(s) 20, 243, 259, 326, 390, 857, 1295
MunI CAATTG 2 cut(s) 750, 1023
Mva1269I GAATGC 1 cut(s) 83
MvaI CCWGG 3 cut(s) 20, 326, 857
MwoI GCNNNNNNNGC 2 cut(s) 662, 1028
NciI CCSGG 4 cut(s) 243, 259, 390, 1295
NdeII GATC 6 cut(s) 100, 320, 411, 1041, 1069, 1248
NlaIII CATG 5 cut(s) 509, 656, 710, 746, 923
NlaIV GGNNCC 5 cut(s) 307, 322, 354, 460, 850
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 2 cut(s) 120, 559
NsiI ATGCAT 1 cut(s) 511
NspI RCATGY 1 cut(s) 710
OliI CACNNNNGTG 2 cut(s) 809, 819
PaqCI CACCTGC 1 cut(s) 237
PciI ACATGT 1 cut(s) 706
PctI GAATGC 1 cut(s) 83
PdmI GAANNNNTTC 2 cut(s) 95, 1147
PfeI GAWTC 4 cut(s) 28, 571, 695, 1298
PfoI TCCNGGA 1 cut(s) 1293
PkrI GCNGC 2 cut(s) 869, 909
PleI GAGTC 2 cut(s) 917, 1149
PpsI GAGTC 2 cut(s) 917, 1149
PscI ACATGT 1 cut(s) 706
Psp6I CCWGG 3 cut(s) 18, 324, 855
PspGI CCWGG 3 cut(s) 18, 324, 855
PspN4I GGNNCC 5 cut(s) 307, 322, 354, 460, 850
PspPI GGNCC 1 cut(s) 16
PstI CTGCAG 1 cut(s) 623
PsuI RGATCY 1 cut(s) 320
RseI CAYNNNNRTG 4 cut(s) 734, 809, 819, 1056
SaqAI TTAA 5 cut(s) 161, 267, 828, 1092, 1308
SatI GCNGC 2 cut(s) 868, 908
Sau3AI GATC 6 cut(s) 100, 320, 411, 1041, 1069, 1248
Sau96I GGNCC 1 cut(s) 16
SchI GAGTC 2 cut(s) 917, 1149
ScrFI CCNGG 7 cut(s) 20, 243, 259, 326, 390, 857, 1295
SduI GDGCHC 2 cut(s) 226, 816
SfaNI GCATC 3 cut(s) 539, 674, 1060
SfcI CTRYAG 1 cut(s) 619
SmiMI CAYNNNNRTG 4 cut(s) 734, 809, 819, 1056
SmlI CTYRAG 2 cut(s) 758, 944
SmoI CTYRAG 2 cut(s) 758, 944
SpeI ACTAGT 1 cut(s) 41
Sse9I AATT 9 cut(s) 345, 494, 514, 539, 750, 783, 1023, 1216, 1342
SsiI CCGC 2 cut(s) 681, 765
SspMI CTAG 3 cut(s) 42, 533, 678
StyD4I CCNGG 7 cut(s) 18, 241, 257, 324, 388, 855, 1293
TaaI ACNGT 5 cut(s) 116, 169, 565, 725, 1281
TaqI TCGA 6 cut(s) 99, 373, 537, 569, 999, 1158
TasI AATT 9 cut(s) 345, 494, 514, 539, 750, 783, 1023, 1216, 1342
TfiI GAWTC 4 cut(s) 28, 571, 695, 1298
Tru1I TTAA 5 cut(s) 161, 267, 828, 1092, 1308
Tru9I TTAA 5 cut(s) 161, 267, 828, 1092, 1308
TscAI CASTG 3 cut(s) 121, 344, 568
TseFI GTSAC 2 cut(s) 120, 559
TseI GCWGC 2 cut(s) 867, 907
Tsp45I GTSAC 2 cut(s) 120, 559
TspDTI ATGAA 6 cut(s) 17, 596, 919, 989, 1074, 1092
TspGWI ACGGA 3 cut(s) 432, 809, 1323
TspRI CASTG 3 cut(s) 121, 344, 568
VneI GTGCAC 1 cut(s) 812
XapI RAATTY 2 cut(s) 539, 1216
XceI RCATGY 1 cut(s) 710
XcmI CCANNNNNNNNNTGG 2 cut(s) 246, 1000
XmiI GTMKAC 1 cut(s) 916
XmnI GAANNNNTTC 2 cut(s) 95, 1147
XspI CTAG 3 cut(s) 42, 533, 678
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.