MD00G1140300.v1.1

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005900
Physical Location & Seq
Forward (+)
30707595 .. 30711043
3449 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1140300.v1.1.491

Sequence Viewer

Length: 1365 bp
ATGAAGAGCCTATTGGCTCTGCTTTTGATTCTGGCATTTACTAGTGCTGCTCAGTATGATGGAGAAGGGAAGGACGGAGAATGCGAAGGGAATAAGCTTTTCGATCCAAGACCTCACAGTGTGTCACTCTTGGAGTTTGGAGCAGTTGGAGACGGGAAAACATTGAACACGGTCGCGTTCCAAAATGCTATTTTCTATGTCAAGTCGTTTGCTGACAAGGGCGGTGCTCAGCTCTATGTTCCGCCCGGAAGGTGGCTTACCGGAAGTTTTAACCTCACCAGCCACCTCACGCTCTTCCTGGAAGCTGGCGCCATCATTCTTGGATCCCAGGATTACACTCACTGGGAAACTGTGGAACCCCTGCCTTCTTATGGTAGAGGGATTGACTTGCCGGGTTGGAGATATCGTAGTTTGATCAACGGAGACAATCTAACCGATGTGATGGTAACAGGTGATAATGGAACCATTGATGGACAGGGTTCCGTCTGGTGGGAACAATTCAGCTATCATGCCTTGAATTATAGCCGACCACATCTAGTGGAATTTACCGGATGTGAAAATGTTACTGTATCGAATTTAACCCTCTTGAACTCCCCTGCTTGGCACATTCATCCGACATACTGCAGCAATGTGCAAGTTCTAAACATAACTCTTCATGCTTTTCATGCATCTCCATATACTAGCGGTATAGTCCCAGATTCTTCAGAAAATGTTTGCATTGAGAACTGCAACATTAGTGTGGGGCATGACGCAATTGTTCTCAAGAGCGGTTGGGATGAGTATGGAATTGCCTTCGGAAAACCAACAAAAAATGTGCACATCCGTGGCGTTAACATTCAGTCTTCTTTAGGCTCTGGCCTGGCATTTGGCAGCGAGATGTCTGGAGGCATATCGAATGTACGTGCAGAGCAGCTTCATCTACATGACTCATTCATCGGCATTGCACTCAAGACAGCTATGGGAAGAGGAGGTTATATGAAAGACATAGTTATATCCAATGTCGAAATGGAGAACATTCACTTGGCAATTGAGGCTACTGGTCAGATCGGTTCGCATCCTGATGAAAAATATGATCCTGATGAACTACCAGTAGTTAAAGGAATCACCTTTGAGAATATGGTAGGTACTAATATTACATTAGTCGGAAACTTTTCTGGACTCAAAAAATCGCCTTTCACATCCATCTGTCTCTCGAATATCTCCTTCTCCCTCACTTGGAATTCGTCTACCTCATGGTTCTGTTCCAACGTGATCGGTTTTTCGCAGGAAGTTTCTCCCAAACCATGTCCAAATCTCCAAGTCTCGGCTTTTAACTCTTCCGCAGCTTGCTTCTTTCCCTTATATAATTATGCTTCAGTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

455

Amino Acids

49.28

Weight (kDa)

5.31

Isoelectric Point (pI)

38.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 137 - 417 4.7e-33 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 308
AccBSI CCGCTC 1 cut(s) 768
AccI GTMKAC 1 cut(s) 1226
AccII CGCG 1 cut(s) 176
AciI CCGC 5 cut(s) 222, 242, 684, 768, 1320
AclWI GGATC 4 cut(s) 98, 318, 331, 1067
AcsI RAATTY 3 cut(s) 542, 574, 1219
AcuI CTGAAG 2 cut(s) 687, 1338
AcyI GRCGYC 1 cut(s) 309
AdeI CACNNNGTG 1 cut(s) 121
AfaI GTAC 2 cut(s) 900, 1126
AfiI CCNNNNNNNGG 6 cut(s) 252, 371, 489, 600, 1215, 1303
AgsI TTSAA 3 cut(s) 166, 517, 589
AhlI ACTAGT 1 cut(s) 41
AjnI CCWGG 3 cut(s) 297, 327, 858
AjuI GAANNNNNNNTTGG 3 cut(s) 28, 1004, 1036
AleI CACNNNNGTG 1 cut(s) 822
AluBI AGCT 7 cut(s) 97, 232, 305, 504, 913, 956, 1325
AluI AGCT 7 cut(s) 97, 232, 305, 504, 913, 956, 1325
Alw21I GWGCWC 2 cut(s) 229, 819
Alw26I GTCTC 4 cut(s) 144, 417, 1193, 1306
Alw44I GTGCAC 1 cut(s) 815
AlwI GGATC 4 cut(s) 98, 318, 331, 1067
AoxI GGCC 1 cut(s) 856
ApaLI GTGCAC 1 cut(s) 815
ApeKI GCWGC 5 cut(s) 47, 624, 870, 910, 1322
ApoI RAATTY 3 cut(s) 542, 574, 1219
Asp700I GAANNNNTTC 1 cut(s) 1150
AspLEI GCGC 1 cut(s) 311
AsuC2I CCSGG 2 cut(s) 246, 393
AsuHPI GGTGA 3 cut(s) 268, 464, 1096
BaeGI GKGCMC 1 cut(s) 819
BamHI GGATCC 1 cut(s) 323
BanI GGYRCC 1 cut(s) 308
BarI GAAGNNNNNNTAC 2 cut(s) 241, 273
BbsI GAAGAC 1 cut(s) 834
Bbv12I GWGCWC 2 cut(s) 229, 819
BbvI GCAGC 5 cut(s) 34, 636, 882, 922, 1334
BccI CCATC 5 cut(s) 53, 320, 436, 464, 1190
BciT130I CCWGG 3 cut(s) 299, 329, 860
BclI TGATCA 1 cut(s) 414
BcnI CCSGG 2 cut(s) 246, 393
BcoDI GTCTC 4 cut(s) 144, 417, 1193, 1306
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 3 cut(s) 42, 536, 681
BfmI CTRYAG 1 cut(s) 622
BfoI RGCGCY 1 cut(s) 312
BisI GCNGC 5 cut(s) 48, 625, 871, 911, 1323
BlpI GCTNAGC 1 cut(s) 228
BlsI GCNGC 5 cut(s) 49, 626, 872, 912, 1324
Bme1390I CCNGG 5 cut(s) 246, 299, 329, 393, 860
BmiI GGNNCC 5 cut(s) 310, 325, 357, 463, 481
BmrFI CCNGG 5 cut(s) 246, 299, 329, 393, 860
BmrI ACTGGG 1 cut(s) 352
BmsI GCATC 2 cut(s) 677, 1063
BmuI ACTGGG 1 cut(s) 352
BpiI GAAGAC 1 cut(s) 834
BpmI CTGGAG 1 cut(s) 903
Bpu1102I GCTNAGC 1 cut(s) 228
BpuEI CTTGAG 2 cut(s) 746, 932
BpuMI CCSGG 2 cut(s) 246, 393
BsaAI YACGTR 1 cut(s) 902
BsaHI GRCGYC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 327, 823
BsaWI WCCGGW 2 cut(s) 260, 548
BsaXI ACNNNNNCTCC 2 cut(s) 391, 421
Bsc4I CCNNNNNNNGG 6 cut(s) 252, 371, 489, 600, 1215, 1303
Bse1I ACTGG 3 cut(s) 347, 1042, 1088
Bse3DI GCAATG 2 cut(s) 634, 939
BseBI CCWGG 3 cut(s) 299, 329, 860
BseDI CCNNGG 2 cut(s) 327, 823
BseGI GGATG 6 cut(s) 557, 610, 781, 819, 1054, 1178
BseLI CCNNNNNNNGG 6 cut(s) 252, 371, 489, 600, 1215, 1303
BseMI GCAATG 2 cut(s) 634, 939
BseMII CTCAG 2 cut(s) 65, 242
BseNI ACTGG 3 cut(s) 347, 1042, 1088
BseRI GAGGAG 1 cut(s) 981
BseSI GKGCMC 1 cut(s) 819
BseXI GCAGC 5 cut(s) 34, 636, 882, 922, 1334
BsgI GTGCAG 1 cut(s) 924
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 858
BshNI GGYRCC 1 cut(s) 308
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 2 cut(s) 229, 819
BsiSI CCGG 4 cut(s) 246, 261, 392, 549
BslFI GGGAC 1 cut(s) 677
BslI CCNNNNNNNGG 6 cut(s) 252, 371, 489, 600, 1215, 1303
BsmAI GTCTC 4 cut(s) 144, 417, 1193, 1306
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 677
BsmI GAATGC 1 cut(s) 86
BsnI GGCC 1 cut(s) 858
Bsp1286I GDGCHC 2 cut(s) 229, 819
Bsp143I GATC 6 cut(s) 103, 323, 414, 1044, 1072, 1251
Bsp1720I GCTNAGC 1 cut(s) 228
BspACI CCGC 5 cut(s) 222, 242, 684, 768, 1320
BspANI GGCC 1 cut(s) 858
BspCNI CTCAG 2 cut(s) 64, 241
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 5 cut(s) 310, 325, 357, 463, 481
BspMAI CTGCAG 1 cut(s) 626
BspPI GGATC 4 cut(s) 98, 318, 331, 1067
BspQI GCTCTTC 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 308
BsrBI CCGCTC 1 cut(s) 768
BsrDI GCAATG 2 cut(s) 634, 939
BsrI ACTGG 3 cut(s) 347, 1042, 1088
BssECI CCNNGG 2 cut(s) 327, 823
BssMI GATC 6 cut(s) 103, 323, 414, 1044, 1072, 1251
BssNI GRCGYC 1 cut(s) 309
Bst2UI CCWGG 3 cut(s) 299, 329, 860
Bst4CI ACNGT 4 cut(s) 119, 172, 352, 568
Bst6I CTCTTC 4 cut(s) 299, 657, 958, 1321
BstACI GRCGYC 1 cut(s) 309
BstBAI YACGTR 1 cut(s) 902
BstC8I GCNNGC 2 cut(s) 307, 1327
BstDEI CTNAG 2 cut(s) 51, 228
BstDSI CCRYGG 1 cut(s) 823
BstF5I GGATG 6 cut(s) 557, 610, 781, 819, 1054, 1178
BstFNI CGCG 1 cut(s) 176
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 6 cut(s) 106, 326, 417, 1047, 1075, 1254
BstMAI GTCTC 4 cut(s) 144, 417, 1193, 1306
BstMBI GATC 6 cut(s) 103, 323, 414, 1044, 1072, 1251
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 2 cut(s) 665, 1031
BstNI CCWGG 3 cut(s) 299, 329, 860
BstSCI CCNGG 5 cut(s) 244, 297, 327, 391, 858
BstSFI CTRYAG 1 cut(s) 622
BstSLI GKGCMC 1 cut(s) 819
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 5 cut(s) 34, 636, 882, 922, 1334
BstV2I GAAGAC 1 cut(s) 834
BstX2I RGATCY 1 cut(s) 323
BstYI RGATCY 1 cut(s) 323
BsuRI GGCC 1 cut(s) 858
BtgI CCRYGG 1 cut(s) 823
BtsCI GGATG 6 cut(s) 557, 610, 781, 819, 1054, 1178
BtsIMutI CAGTG 2 cut(s) 124, 340
Cac8I GCNNGC 2 cut(s) 307, 1327
CfoI GCGC 1 cut(s) 311
CseI GACGC 1 cut(s) 758
Csp6I GTAC 2 cut(s) 899, 1125
CviAII CATG 7 cut(s) 509, 656, 665, 746, 923, 1233, 1284
CviQI GTAC 2 cut(s) 899, 1125
DdeI CTNAG 2 cut(s) 51, 228
DinI GGCGCC 1 cut(s) 310
DpnI GATC 6 cut(s) 105, 325, 416, 1046, 1074, 1253
DpnII GATC 6 cut(s) 103, 323, 414, 1044, 1072, 1251
DraIII CACNNNGTG 1 cut(s) 121
Eam1104I CTCTTC 4 cut(s) 299, 657, 958, 1321
EarI CTCTTC 4 cut(s) 299, 657, 958, 1321
EciI GGCGGA 1 cut(s) 231
Eco32I GATATC 1 cut(s) 404
Eco57I CTGAAG 2 cut(s) 687, 1338
EcoRI GAATTC 1 cut(s) 1219
EcoRII CCWGG 3 cut(s) 297, 327, 858
EcoRV GATATC 1 cut(s) 404
EcoT22I ATGCAT 1 cut(s) 670
EgeI GGCGCC 1 cut(s) 310
EheI GGCGCC 1 cut(s) 310
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 7 cut(s) 512, 659, 668, 749, 926, 1236, 1287
FaqI GGGAC 1 cut(s) 677
FatI CATG 7 cut(s) 508, 655, 664, 745, 922, 1232, 1283
FbaI TGATCA 1 cut(s) 414
FblI GTMKAC 1 cut(s) 1226
Fnu4HI GCNGC 5 cut(s) 48, 625, 871, 911, 1323
FokI GGATG 6 cut(s) 564, 597, 788, 806, 1041, 1165
Fsp4HI GCNGC 5 cut(s) 48, 625, 871, 911, 1323
FspBI CTAG 3 cut(s) 42, 536, 681
GlaI GCGC 1 cut(s) 310
GluI GCNGC 5 cut(s) 48, 625, 871, 911, 1323
GsuI CTGGAG 1 cut(s) 903
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 1 cut(s) 858
HapII CCGG 4 cut(s) 246, 261, 392, 549
HgaI GACGC 1 cut(s) 758
HhaI GCGC 1 cut(s) 311
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 7 cut(s) 512, 659, 668, 749, 926, 1236, 1287
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 5 cut(s) 28, 698, 926, 1101, 1158
HpaI GTTAAC 1 cut(s) 832
HpaII CCGG 4 cut(s) 246, 261, 392, 549
HphI GGTGA 3 cut(s) 268, 464, 1096
Hpy166II GTNNAC 3 cut(s) 817, 832, 1227
Hpy188I TCNGA 5 cut(s) 615, 706, 797, 1044, 1145
Hpy188III TCNNGA 8 cut(s) 586, 763, 882, 949, 1058, 1076, 1155, 1192
Hpy8I GTNNAC 3 cut(s) 817, 832, 1227
HpyAV CCTTC 7 cut(s) 59, 64, 80, 243, 375, 802, 1213
HpyCH4III ACNGT 4 cut(s) 119, 172, 352, 568
HpyCH4IV ACGT 2 cut(s) 901, 1248
HpyCH4V TGCA 8 cut(s) 624, 634, 668, 717, 729, 817, 905, 944
HpyF10VI GCNNNNNNNGC 2 cut(s) 665, 1031
HpyF3I CTNAG 2 cut(s) 51, 228
HpySE526I ACGT 2 cut(s) 901, 1248
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 7 cut(s) 512, 659, 668, 749, 926, 1236, 1287
HspAI GCGC 1 cut(s) 309
KasI GGCGCC 1 cut(s) 308
Ksp22I TGATCA 1 cut(s) 414
KspAI GTTAAC 1 cut(s) 832
Kzo9I GATC 6 cut(s) 103, 323, 414, 1044, 1072, 1251
LguI GCTCTTC 1 cut(s) 299
LmnI GCTCC 1 cut(s) 140
Lsp1109I GCAGC 5 cut(s) 34, 636, 882, 922, 1334
LweI GCATC 2 cut(s) 677, 1063
MaeI CTAG 3 cut(s) 42, 536, 681
MaeII ACGT 2 cut(s) 901, 1248
MaeIII GTNAC 3 cut(s) 123, 445, 562
MalI GATC 6 cut(s) 105, 325, 416, 1046, 1074, 1253
MbiI CCGCTC 1 cut(s) 768
MboI GATC 6 cut(s) 103, 323, 414, 1044, 1072, 1251
MboII GAAGA 7 cut(s) 16, 286, 644, 693, 834, 975, 1308
MfeI CAATTG 2 cut(s) 753, 1026
MflI RGATCY 1 cut(s) 323
MhlI GDGCHC 2 cut(s) 229, 819
MluCI AATT 9 cut(s) 497, 517, 542, 574, 753, 786, 1026, 1219, 1345
Mly113I GGCGCC 1 cut(s) 309
MlyI GAGTC 2 cut(s) 920, 1152
MmeI TCCRAC 5 cut(s) 127, 377, 638, 1123, 1269
Mph1103I ATGCAT 1 cut(s) 670
MroXI GAANNNNTTC 1 cut(s) 1150
MseI TTAA 5 cut(s) 270, 578, 831, 1095, 1311
MslI CAYNNNNRTG 4 cut(s) 737, 822, 921, 1059
MspI CCGG 4 cut(s) 246, 261, 392, 549
MspR9I CCNGG 5 cut(s) 246, 299, 329, 393, 860
MunI CAATTG 2 cut(s) 753, 1026
Mva1269I GAATGC 1 cut(s) 86
MvaI CCWGG 3 cut(s) 299, 329, 860
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 665, 1031
NarI GGCGCC 1 cut(s) 309
NciI CCSGG 2 cut(s) 246, 393
NdeII GATC 6 cut(s) 103, 323, 414, 1044, 1072, 1251
NlaIII CATG 7 cut(s) 512, 659, 668, 749, 926, 1236, 1287
NlaIV GGNNCC 5 cut(s) 310, 325, 357, 463, 481
NmeAIII GCCGAG 1 cut(s) 1283
NmuCI GTSAC 1 cut(s) 123
NsiI ATGCAT 1 cut(s) 670
OliI CACNNNNGTG 1 cut(s) 822
PciSI GCTCTTC 1 cut(s) 299
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 1 cut(s) 86
PdmI GAANNNNTTC 1 cut(s) 1150
PfeI GAWTC 3 cut(s) 28, 698, 1101
PfoI TCCNGGA 1 cut(s) 297
PkrI GCNGC 5 cut(s) 49, 626, 872, 912, 1324
PleI GAGTC 2 cut(s) 920, 1152
PluTI GGCGCC 1 cut(s) 312
PpsI GAGTC 2 cut(s) 920, 1152
Ppu21I YACGTR 1 cut(s) 902
Psp6I CCWGG 3 cut(s) 297, 327, 858
PspGI CCWGG 3 cut(s) 297, 327, 858
PspN4I GGNNCC 5 cut(s) 310, 325, 357, 463, 481
PstI CTGCAG 1 cut(s) 626
PsuI RGATCY 1 cut(s) 323
RsaI GTAC 2 cut(s) 900, 1126
RsaNI GTAC 2 cut(s) 899, 1125
RseI CAYNNNNRTG 4 cut(s) 737, 822, 921, 1059
SapI GCTCTTC 1 cut(s) 299
SaqAI TTAA 5 cut(s) 270, 578, 831, 1095, 1311
SatI GCNGC 5 cut(s) 48, 625, 871, 911, 1323
Sau3AI GATC 6 cut(s) 103, 323, 414, 1044, 1072, 1251
SchI GAGTC 2 cut(s) 920, 1152
ScrFI CCNGG 5 cut(s) 246, 299, 329, 393, 860
SduI GDGCHC 2 cut(s) 229, 819
SfaNI GCATC 2 cut(s) 677, 1063
SfcI CTRYAG 1 cut(s) 622
SfoI GGCGCC 1 cut(s) 310
SmiMI CAYNNNNRTG 4 cut(s) 737, 822, 921, 1059
SmlI CTYRAG 2 cut(s) 761, 947
SmoI CTYRAG 2 cut(s) 761, 947
SpeI ACTAGT 1 cut(s) 41
Sse9I AATT 9 cut(s) 497, 517, 542, 574, 753, 786, 1026, 1219, 1345
SsiI CCGC 5 cut(s) 222, 242, 684, 768, 1320
SspDI GGCGCC 1 cut(s) 308
SspI AATATT 1 cut(s) 1132
SspMI CTAG 3 cut(s) 42, 536, 681
StyD4I CCNGG 5 cut(s) 244, 297, 327, 391, 858
TaaI ACNGT 4 cut(s) 119, 172, 352, 568
TaiI ACGT 2 cut(s) 904, 1251
TaqI TCGA 5 cut(s) 102, 572, 893, 1002, 1193
TasI AATT 9 cut(s) 497, 517, 542, 574, 753, 786, 1026, 1219, 1345
TfiI GAWTC 3 cut(s) 28, 698, 1101
Tru1I TTAA 5 cut(s) 270, 578, 831, 1095, 1311
Tru9I TTAA 5 cut(s) 270, 578, 831, 1095, 1311
TscAI CASTG 2 cut(s) 124, 347
TseFI GTSAC 1 cut(s) 123
TseI GCWGC 5 cut(s) 47, 624, 870, 910, 1322
Tsp45I GTSAC 1 cut(s) 123
TspDTI ATGAA 9 cut(s) 17, 599, 644, 653, 905, 922, 992, 1077, 1095
TspGWI ACGGA 4 cut(s) 90, 435, 472, 812
TspRI CASTG 2 cut(s) 124, 347
VneI GTGCAC 1 cut(s) 815
XapI RAATTY 3 cut(s) 542, 574, 1219
XcmI CCANNNNNNNNNTGG 1 cut(s) 1003
XmiI GTMKAC 1 cut(s) 1226
XmnI GAANNNNTTC 1 cut(s) 1150
XspI CTAG 3 cut(s) 42, 536, 681
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.