Rh6AG358100

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0005900
Physical Location & Seq
Forward (+)
55688312 .. 55691410
3099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG358100.1

Sequence Viewer

Length: 1413 bp
ATGAAGAGGCTAGTAAGTAGGCATCCAACTGTGGTTCAACCTTTTCTTGTTGTTTTTTCTTATTTTCAGTTGGCGCTGGTTTTGGTTCTGGGATTTACTAGTGCATTACAGTACGATGGAGAATGTGAAAACAAGAAGGCATTGAATCCCAGGCCACACAGTGTGTCGCTTTTGGAGTTTGGGGCAGTCGGAGATGGGAAAACATTGAACACAGTAGCTTTCCAAAATGCCGTTTTCTATGTCAAGTCATTTGCTGACAAGGGTGGTGCTCAGCTCTATGTTCCGCCTGGCCGGTGGCTCACTGGGAGCTTTAACCTCACCAGCCACCTCACGCTCTTCCTTGAGACCGGTGCAACTATTCTTGGATCCCAGGACTACACTCACTGGGAAATTGAGGAACCCCTTCCTTCTTATGGTCGCGGGATTGATTTTCCAGGTTGGAGATATCGTAGCTTGATCAACGGTGATGATCTACACGATGTGGTGATAACAGGTGGTAATGGAACCATAGATGGACAGGGTGCTGTGTGGTGGGAGCAATTCAGCTCTCATTCGTTGAATTACAGCAGACCACATCTTGTGGAATTCACCAGATGTGATAATATCGTTATATCGAATCTGACTTTCTTGAACTCCCCGGCTTGGAACATTCATCCTACATATTGCAGGTATGTGAAAGTCCAAAACTTAACTGTTCATGCTTCTCCTGCATCTCCATATACTAGCGGAATAGTCCCAGATTCTTCTGAGCATGTTTGCATTGAGAACTGCAACATTAGTATGGGTCATGATGCAATTGTTCTTAAGAGCGGTTGGGATGAGTATGGAATCGCCTATGGCAAACCGACCAAAAGTGTACACATCCGGGCCGTCAACCTTCAATCTTCTTTAGGCTCCGGCCTGGCATTTGGGAGTGAGATGTCTGGAGGCATTTTAAACGTACTAGTAGAGCGGCTTCATCTGTCTAACTCATTCGTTGGCATTGAACTCAAGACAACCAGAGGAAGAGGAGGATATATGAAAGACATTGTTATATCGAATGTAGAAATGCACAACATTCACACAGCAATTCAGGTCACTGGTCAGAGTGGCTCTCATCCTGATGATAAGTTTGACCCTGATCAAGTTCCATTTGTTAAAGGAATCACCTTTGACAACATGGTCGGCAGTAATATTACATTTGCTGGGAATTTTTCTGGAATCTACGAATCACCTTTCACATCTATTTGTCTCTCAAATGTGTCCTTCTCCATGACTTGGGATTCTTCTAGCTCATGGTTCTGTTCCGATGTGAAGGGGTTTTCGCAGAAGGTTTCTCCAGAACCATGTTCAGACCTCAAGGAATCATCTTTTAATGCTTCCTCATCTTGCTTCTTTTCCTTATACCCTTACAATTATGCTACAGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

470

Amino Acids

51.73

Weight (kDa)

5.78

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 151 - 432 1.9e-32 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1160
Acc36I ACCTGC 1 cut(s) 657
AccB7I CCANNNNNTGG 1 cut(s) 1257
AccBSI CCGCTC 2 cut(s) 810, 952
AccII CGCG 1 cut(s) 420
AciI CCGC 5 cut(s) 284, 420, 726, 810, 952
AclWI GGATC 2 cut(s) 360, 373
AcoI YGGCCR 1 cut(s) 289
AcsI RAATTY 2 cut(s) 584, 1189
AdeI CACNNNGTG 3 cut(s) 161, 163, 481
AfaI GTAC 3 cut(s) 113, 858, 942
AfiI CCNNNNNNNGG 3 cut(s) 413, 642, 1257
AflII CTTAAG 1 cut(s) 803
AgeI ACCGGT 1 cut(s) 347
AgsI TTSAA 7 cut(s) 38, 145, 208, 559, 631, 881, 986
AhlI ACTAGT 2 cut(s) 98, 943
AjnI CCWGG 5 cut(s) 149, 286, 369, 433, 900
AluBI AGCT 6 cut(s) 218, 274, 309, 453, 546, 1272
AluI AGCT 6 cut(s) 218, 274, 309, 453, 546, 1272
Alw21I GWGCWC 1 cut(s) 271
Alw26I GTCTC 2 cut(s) 338, 1235
AlwI GGATC 2 cut(s) 360, 373
AoxI GGCC 4 cut(s) 152, 289, 867, 898
ApoI RAATTY 2 cut(s) 584, 1189
AsiGI ACCGGT 1 cut(s) 347
Asp700I GAANNNNTTC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 1 cut(s) 867
AsuC2I CCSGG 2 cut(s) 638, 866
AsuHPI GGTGA 6 cut(s) 310, 476, 496, 580, 1138, 1203
BamHI GGATCC 1 cut(s) 365
BarI GAAGNNNNNNTAC 2 cut(s) 939, 971
Bbv12I GWGCWC 1 cut(s) 271
BccI CCATC 3 cut(s) 110, 188, 506
BceAI ACGGC 2 cut(s) 215, 854
BciT130I CCWGG 5 cut(s) 151, 288, 371, 435, 902
BclI TGATCA 2 cut(s) 456, 1120
BcnI CCSGG 2 cut(s) 638, 866
BcoDI GTCTC 2 cut(s) 338, 1235
BcuI ACTAGT 2 cut(s) 98, 943
BfaI CTAG 5 cut(s) 11, 99, 723, 944, 1269
BfmI CTRYAG 1 cut(s) 1401
BfoI RGCGCY 1 cut(s) 77
BfrI CTTAAG 1 cut(s) 803
BfuAI ACCTGC 1 cut(s) 657
BisI GCNGC 1 cut(s) 953
BlpI GCTNAGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 954
Bme1390I CCNGG 7 cut(s) 151, 288, 371, 435, 638, 866, 902
BmgT120I GGNCC 1 cut(s) 867
BmiI GGNNCC 4 cut(s) 367, 399, 505, 895
BmrFI CCNGG 7 cut(s) 151, 288, 371, 435, 638, 866, 902
BmrI ACTGGG 2 cut(s) 312, 394
BmsI GCATC 3 cut(s) 31, 719, 781
BmuI ACTGGG 2 cut(s) 312, 394
BplI GAGNNNNNCTC 2 cut(s) 1078, 1110
BpmI CTGGAG 2 cut(s) 945, 1302
Bpu1102I GCTNAGC 1 cut(s) 270
BpuEI CTTGAG 3 cut(s) 362, 974, 1322
BpuMI CCSGG 2 cut(s) 638, 866
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 3 cut(s) 149, 369, 636
BsaWI WCCGGW 1 cut(s) 347
BsaXI ACNNNNNCTCC 2 cut(s) 433, 463
Bsc4I CCNNNNNNNGG 3 cut(s) 413, 642, 1257
Bse118I RCCGGY 2 cut(s) 291, 347
Bse1I ACTGG 3 cut(s) 307, 389, 1084
BseBI CCWGG 5 cut(s) 151, 288, 371, 435, 902
BseDI CCNNGG 3 cut(s) 149, 369, 636
BseGI GGATG 5 cut(s) 22, 652, 823, 861, 1096
BseLI CCNNNNNNNGG 3 cut(s) 413, 642, 1257
BseMII CTCAG 2 cut(s) 284, 738
BseNI ACTGG 3 cut(s) 307, 389, 1084
BseRI GAGGAG 1 cut(s) 1023
BseYI CCCAGC 1 cut(s) 1184
Bsh1236I CGCG 1 cut(s) 420
BshFI GGCC 4 cut(s) 154, 291, 869, 900
BshTI ACCGGT 1 cut(s) 347
BsiHKAI GWGCWC 1 cut(s) 271
BsiSI CCGG 5 cut(s) 292, 348, 638, 865, 897
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 3 cut(s) 413, 642, 1257
BsmAI GTCTC 2 cut(s) 338, 1235
BsmFI GGGAC 1 cut(s) 719
BsnI GGCC 4 cut(s) 154, 291, 869, 900
Bso31I GGTCTC 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 271
Bsp1407I TGTACA 1 cut(s) 856
Bsp143I GATC 4 cut(s) 365, 456, 469, 1120
Bsp1720I GCTNAGC 1 cut(s) 270
BspACI CCGC 5 cut(s) 284, 420, 726, 810, 952
BspANI GGCC 4 cut(s) 154, 291, 869, 900
BspCNI CTCAG 2 cut(s) 283, 739
BspFNI CGCG 1 cut(s) 420
BspHI TCATGA 1 cut(s) 787
BspLI GGNNCC 4 cut(s) 367, 399, 505, 895
BspMI ACCTGC 1 cut(s) 657
BspPI GGATC 2 cut(s) 360, 373
BspQI GCTCTTC 1 cut(s) 341
BspTI CTTAAG 1 cut(s) 803
BspTNI GGTCTC 1 cut(s) 338
BsrBI CCGCTC 2 cut(s) 810, 952
BsrFI RCCGGY 2 cut(s) 291, 347
BsrGI TGTACA 1 cut(s) 856
BsrI ACTGG 3 cut(s) 307, 389, 1084
BssAI RCCGGY 2 cut(s) 291, 347
BssECI CCNNGG 3 cut(s) 149, 369, 636
BssMI GATC 4 cut(s) 365, 456, 469, 1120
Bst2UI CCWGG 5 cut(s) 151, 288, 371, 435, 902
Bst4CI ACNGT 6 cut(s) 31, 111, 161, 214, 464, 694
Bst6I CTCTTC 2 cut(s) 341, 1000
BstAFI CTTAAG 1 cut(s) 803
BstAUI TGTACA 1 cut(s) 856
BstDEI CTNAG 2 cut(s) 270, 747
BstF5I GGATG 5 cut(s) 22, 652, 823, 861, 1096
BstFNI CGCG 1 cut(s) 420
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 4 cut(s) 368, 459, 472, 1123
BstMAI GTCTC 2 cut(s) 338, 1235
BstMBI GATC 4 cut(s) 365, 456, 469, 1120
BstMWI GCNNNNNNNGC 1 cut(s) 707
BstNI CCWGG 5 cut(s) 151, 288, 371, 435, 902
BstNSI RCATGY 1 cut(s) 755
BstSCI CCNGG 7 cut(s) 149, 286, 369, 433, 636, 864, 900
BstSFI CTRYAG 1 cut(s) 1401
BstUI CGCG 1 cut(s) 420
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
BsuRI GGCC 4 cut(s) 154, 291, 869, 900
BtsCI GGATG 5 cut(s) 22, 652, 823, 861, 1096
BtsIMutI CAGTG 4 cut(s) 166, 300, 382, 1077
BveI ACCTGC 1 cut(s) 657
CciI TCATGA 1 cut(s) 787
CfoI GCGC 1 cut(s) 76
Cfr10I RCCGGY 2 cut(s) 291, 347
Cfr13I GGNCC 1 cut(s) 867
Csp6I GTAC 3 cut(s) 112, 857, 941
CspAI ACCGGT 1 cut(s) 347
CviAII CATG 7 cut(s) 698, 752, 788, 1159, 1252, 1275, 1326
CviQI GTAC 3 cut(s) 112, 857, 941
DdeI CTNAG 2 cut(s) 270, 747
DpnI GATC 4 cut(s) 367, 458, 471, 1122
DpnII GATC 4 cut(s) 365, 456, 469, 1120
DraI TTTAAA 1 cut(s) 936
DraIII CACNNNGTG 3 cut(s) 161, 163, 481
DrdI GACNNNNNNGTC 1 cut(s) 1160
DseDI GACNNNNNNGTC 1 cut(s) 1160
EaeI YGGCCR 1 cut(s) 289
Eam1104I CTCTTC 2 cut(s) 341, 1000
EarI CTCTTC 2 cut(s) 341, 1000
EciI GGCGGA 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 338
Eco32I GATATC 1 cut(s) 446
EcoRI GAATTC 1 cut(s) 584
EcoRII CCWGG 5 cut(s) 149, 286, 369, 433, 900
EcoRV GATATC 1 cut(s) 446
FaeI CATG 7 cut(s) 701, 755, 791, 1162, 1255, 1278, 1329
FaqI GGGAC 1 cut(s) 719
FatI CATG 7 cut(s) 697, 751, 787, 1158, 1251, 1274, 1325
FauI CCCGC 1 cut(s) 413
FbaI TGATCA 2 cut(s) 456, 1120
Fnu4HI GCNGC 1 cut(s) 953
FokI GGATG 5 cut(s) 9, 639, 830, 848, 1083
Fsp4HI GCNGC 1 cut(s) 953
FspBI CTAG 5 cut(s) 11, 99, 723, 944, 1269
GlaI GCGC 1 cut(s) 75
GluI GCNGC 1 cut(s) 953
GsaI CCCAGC 1 cut(s) 1188
GsuI CTGGAG 2 cut(s) 945, 1302
HaeII RGCGCY 1 cut(s) 77
HaeIII GGCC 4 cut(s) 154, 291, 869, 900
HapII CCGG 5 cut(s) 292, 348, 638, 865, 897
HhaI GCGC 1 cut(s) 76
Hin1II CATG 7 cut(s) 701, 755, 791, 1162, 1255, 1278, 1329
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HincII GTYRAC 1 cut(s) 874
HindII GTYRAC 1 cut(s) 874
HinfI GANTC 9 cut(s) 145, 616, 740, 828, 1143, 1200, 1208, 1262, 1343
HpaII CCGG 5 cut(s) 292, 348, 638, 865, 897
HphI GGTGA 6 cut(s) 310, 476, 496, 580, 1138, 1203
Hpy166II GTNNAC 3 cut(s) 857, 859, 874
Hpy188I TCNGA 6 cut(s) 191, 621, 748, 1086, 1288, 1333
Hpy188III TCNNGA 7 cut(s) 628, 788, 924, 991, 1100, 1197, 1319
Hpy8I GTNNAC 3 cut(s) 857, 859, 874
HpyAV CCTTC 7 cut(s) 130, 413, 417, 887, 1255, 1288, 1303
HpyCH4III ACNGT 6 cut(s) 31, 111, 161, 214, 464, 694
HpyCH4IV ACGT 1 cut(s) 939
HpyCH4V TGCA 8 cut(s) 104, 353, 666, 710, 759, 771, 794, 1051
HpyF10VI GCNNNNNNNGC 1 cut(s) 707
HpyF3I CTNAG 2 cut(s) 270, 747
HpySE526I ACGT 1 cut(s) 939
Hsp92II CATG 7 cut(s) 701, 755, 791, 1162, 1255, 1278, 1329
HspAI GCGC 1 cut(s) 74
Ksp22I TGATCA 2 cut(s) 456, 1120
Kzo9I GATC 4 cut(s) 365, 456, 469, 1120
LguI GCTCTTC 1 cut(s) 341
LmnI GCTCC 3 cut(s) 306, 535, 899
LweI GCATC 3 cut(s) 31, 719, 781
MaeI CTAG 5 cut(s) 11, 99, 723, 944, 1269
MaeII ACGT 1 cut(s) 939
MaeIII GTNAC 1 cut(s) 1075
MalI GATC 4 cut(s) 367, 458, 471, 1122
MbiI CCGCTC 2 cut(s) 810, 952
MboI GATC 4 cut(s) 365, 456, 469, 1120
MboII GAAGA 6 cut(s) 16, 328, 735, 876, 1017, 1257
MfeI CAATTG 1 cut(s) 795
MflI RGATCY 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 271
MluCI AATT 8 cut(s) 390, 539, 559, 584, 795, 1068, 1189, 1393
MmeI TCCRAC 3 cut(s) 50, 169, 419
MnlI CCTC 9 cut(s) 326, 338, 388, 920, 995, 1001, 1004, 1346, 1372
MroXI GAANNNNTTC 1 cut(s) 402
MseI TTAA 6 cut(s) 312, 689, 804, 935, 1137, 1353
MslI CAYNNNNRTG 2 cut(s) 779, 1101
MspCI CTTAAG 1 cut(s) 803
MspI CCGG 5 cut(s) 292, 348, 638, 865, 897
MspR9I CCNGG 7 cut(s) 151, 288, 371, 435, 638, 866, 902
MunI CAATTG 1 cut(s) 795
MvaI CCWGG 5 cut(s) 151, 288, 371, 435, 902
MvnI CGCG 1 cut(s) 420
MwoI GCNNNNNNNGC 1 cut(s) 707
NciI CCSGG 2 cut(s) 638, 866
NdeII GATC 4 cut(s) 365, 456, 469, 1120
NlaIII CATG 7 cut(s) 701, 755, 791, 1162, 1255, 1278, 1329
NlaIV GGNNCC 4 cut(s) 367, 399, 505, 895
NmuCI GTSAC 1 cut(s) 1075
NspI RCATGY 1 cut(s) 755
PagI TCATGA 1 cut(s) 787
PciSI GCTCTTC 1 cut(s) 341
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 9 cut(s) 145, 616, 740, 828, 1143, 1200, 1208, 1262, 1343
PflMI CCANNNNNTGG 1 cut(s) 1257
PinAI ACCGGT 1 cut(s) 347
PkrI GCNGC 1 cut(s) 954
Psp6I CCWGG 5 cut(s) 149, 286, 369, 433, 900
PspFI CCCAGC 1 cut(s) 1184
PspGI CCWGG 5 cut(s) 149, 286, 369, 433, 900
PspN4I GGNNCC 4 cut(s) 367, 399, 505, 895
PspPI GGNCC 1 cut(s) 867
PsuI RGATCY 1 cut(s) 365
RsaI GTAC 3 cut(s) 113, 858, 942
RsaNI GTAC 3 cut(s) 112, 857, 941
RseI CAYNNNNRTG 2 cut(s) 779, 1101
SapI GCTCTTC 1 cut(s) 341
SaqAI TTAA 6 cut(s) 312, 689, 804, 935, 1137, 1353
SatI GCNGC 1 cut(s) 953
Sau3AI GATC 4 cut(s) 365, 456, 469, 1120
Sau96I GGNCC 1 cut(s) 867
ScrFI CCNGG 7 cut(s) 151, 288, 371, 435, 638, 866, 902
SduI GDGCHC 1 cut(s) 271
SfaNI GCATC 3 cut(s) 31, 719, 781
SfcI CTRYAG 1 cut(s) 1401
SmiMI CAYNNNNRTG 2 cut(s) 779, 1101
SmlI CTYRAG 4 cut(s) 341, 803, 989, 1337
SmoI CTYRAG 4 cut(s) 341, 803, 989, 1337
SpeI ACTAGT 2 cut(s) 98, 943
Sse9I AATT 8 cut(s) 390, 539, 559, 584, 795, 1068, 1189, 1393
SsiI CCGC 5 cut(s) 284, 420, 726, 810, 952
SspI AATATT 1 cut(s) 1174
SspMI CTAG 5 cut(s) 11, 99, 723, 944, 1269
StyD4I CCNGG 7 cut(s) 149, 286, 369, 433, 636, 864, 900
TaaI ACNGT 6 cut(s) 31, 111, 161, 214, 464, 694
TaiI ACGT 1 cut(s) 942
TaqI TCGA 2 cut(s) 614, 1037
TasI AATT 8 cut(s) 390, 539, 559, 584, 795, 1068, 1189, 1393
TatI WGTACW 1 cut(s) 856
TauI GCSGC 1 cut(s) 955
TfiI GAWTC 9 cut(s) 145, 616, 740, 828, 1143, 1200, 1208, 1262, 1343
Tru1I TTAA 6 cut(s) 312, 689, 804, 935, 1137, 1353
Tru9I TTAA 6 cut(s) 312, 689, 804, 935, 1137, 1353
TscAI CASTG 4 cut(s) 166, 307, 389, 1084
TseFI GTSAC 1 cut(s) 1075
Tsp45I GTSAC 1 cut(s) 1075
TspDTI ATGAA 5 cut(s) 17, 641, 686, 947, 1034
TspRI CASTG 4 cut(s) 166, 307, 389, 1084
Van91I CCANNNNNTGG 1 cut(s) 1257
Vha464I CTTAAG 1 cut(s) 803
XapI RAATTY 2 cut(s) 584, 1189
XceI RCATGY 1 cut(s) 755
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 5 cut(s) 11, 99, 723, 944, 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.