FvH4_2g26151

Major allergen Pru ar

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009655
Physical Location & Seq
Reverse (-)
21026500 .. 21027722
1223 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26151.t1

Sequence Viewer

Length: 483 bp
ATGGCTGTCATCAATTTGAGCCAGGAGTTTACTAGTCCAGTAGATGCAGCAAGATTGTTCAAGGCCTTGATCATCGACTCCCACAACCTCATCCCAAAACTCATGCCTCAGGCCATTAAGAGCATCGAAATCATTGAGGGCGACGGAGGAGCTGGAACCATAAAAAAGATCAATTTTGCTGAGGGTACTCGTTTCAAGTACATGATTAACAGAATAGACACCTTGGATGTAGAAAACTTGATGTGCAAGTACACTCTGATTGAAGGCGATGTGTTGGGGGACAAGATTAATTCTATTGCTTACGAGGTGAAGTTCATACCTTCTGCAATTGGAGGATGTGTTTGTAAGATGATGAGCGAGTACCAAGCTGTTGGTGACTTTGAGATCAATGAGGAAGAGATCAGGGGTGGCAAGGAAAGAGCAATGGGCATGTACAAAGTAGTTGAAGCTTACCTCTTCGAAAGTCCTGGTGCTTACGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

161

Amino Acids

17.76

Weight (kDa)

4.85

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 3 - 153 3.3e-24 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 5 cut(s) 187, 200, 251, 362, 434
AgsI TTSAA 4 cut(s) 61, 196, 263, 446
AhlI ACTAGT 1 cut(s) 32
AjnI CCWGG 2 cut(s) 21, 466
AluBI AGCT 3 cut(s) 152, 368, 449
AluI AGCT 3 cut(s) 152, 368, 449
AoxI GGCC 2 cut(s) 63, 111
ApeKI GCWGC 1 cut(s) 47
AseI ATTAAT 1 cut(s) 288
AsuHPI GGTGA 2 cut(s) 319, 386
AsuII TTCGAA 1 cut(s) 459
AxyI CCTNAGG 1 cut(s) 108
BbvCI CCTCAGC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 59
BciT130I CCWGG 2 cut(s) 23, 468
BclI TGATCA 1 cut(s) 69
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 1 cut(s) 33
BisI GCNGC 1 cut(s) 48
BlsI GCNGC 1 cut(s) 49
Bme1390I CCNGG 2 cut(s) 23, 468
BmiI GGNNCC 1 cut(s) 157
BmrFI CCNGG 2 cut(s) 23, 468
BmsI GCATC 2 cut(s) 34, 132
Bpu10I CCTNAGC 1 cut(s) 180
Bpu14I TTCGAA 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 222
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bse1I ACTGG 1 cut(s) 38
Bse21I CCTNAGG 1 cut(s) 108
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 2 cut(s) 23, 468
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 3 cut(s) 90, 232, 341
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 2 cut(s) 122, 171
BseNI ACTGG 1 cut(s) 38
BseRI GAGGAG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 59
BshFI GGCC 2 cut(s) 65, 113
BslFI GGGAC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 293
BsnI GGCC 2 cut(s) 65, 113
Bsp119I TTCGAA 1 cut(s) 459
Bsp1407I TGTACA 1 cut(s) 432
Bsp143I GATC 4 cut(s) 69, 168, 384, 399
BspANI GGCC 2 cut(s) 65, 113
BspCNI CTCAG 2 cut(s) 121, 172
BspLI GGNNCC 1 cut(s) 157
BspT104I TTCGAA 1 cut(s) 459
BsrDI GCAATG 1 cut(s) 429
BsrGI TGTACA 1 cut(s) 432
BsrI ACTGG 1 cut(s) 38
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 4 cut(s) 69, 168, 384, 399
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 2 cut(s) 23, 468
Bst6I CTCTTC 2 cut(s) 390, 461
BstAUI TGTACA 1 cut(s) 432
BstBI TTCGAA 1 cut(s) 459
BstDEI CTNAG 2 cut(s) 108, 180
BstF5I GGATG 3 cut(s) 90, 232, 341
BstKTI GATC 4 cut(s) 72, 171, 387, 402
BstMBI GATC 4 cut(s) 69, 168, 384, 399
BstNI CCWGG 2 cut(s) 23, 468
BstNSI RCATGY 1 cut(s) 433
BstSCI CCNGG 2 cut(s) 21, 466
BstV1I GCAGC 1 cut(s) 59
BstXI CCANNNNNNTGG 1 cut(s) 371
Bsu36I CCTNAGG 1 cut(s) 108
BsuRI GGCC 2 cut(s) 65, 113
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 3 cut(s) 90, 232, 341
Csp6I GTAC 5 cut(s) 186, 199, 250, 361, 433
CviAII CATG 3 cut(s) 103, 202, 430
CviJI RGCY 7 cut(s) 5, 21, 65, 113, 152, 368, 449
CviKI_1 RGCY 7 cut(s) 5, 21, 65, 113, 152, 368, 449
CviQI GTAC 5 cut(s) 186, 199, 250, 361, 433
DdeI CTNAG 2 cut(s) 108, 180
DpnI GATC 4 cut(s) 71, 170, 386, 401
DpnII GATC 4 cut(s) 69, 168, 384, 399
Eam1104I CTCTTC 2 cut(s) 390, 461
EarI CTCTTC 2 cut(s) 390, 461
Eco130I CCWWGG 1 cut(s) 222
Eco147I AGGCCT 1 cut(s) 65
Eco81I CCTNAGG 1 cut(s) 108
EcoRII CCWGG 2 cut(s) 21, 466
EcoT14I CCWWGG 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 3 cut(s) 106, 205, 433
FaiI YATR 5 cut(s) 104, 161, 203, 317, 431
FaqI GGGAC 1 cut(s) 293
FatI CATG 3 cut(s) 102, 201, 429
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 48
FokI GGATG 3 cut(s) 77, 239, 348
Fsp4HI GCNGC 1 cut(s) 48
FspBI CTAG 1 cut(s) 33
GluI GCNGC 1 cut(s) 48
HaeIII GGCC 2 cut(s) 65, 113
Hin1II CATG 3 cut(s) 106, 205, 433
HindIII AAGCTT 1 cut(s) 447
HinfI GANTC 1 cut(s) 77
HphI GGTGA 2 cut(s) 319, 386
Hpy166II GTNNAC 2 cut(s) 30, 252
Hpy188I TCNGA 1 cut(s) 258
Hpy8I GTNNAC 2 cut(s) 30, 252
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 2 cut(s) 257, 330
HpyCH4V TGCA 3 cut(s) 47, 246, 326
HpyF3I CTNAG 2 cut(s) 108, 180
Hsp92II CATG 3 cut(s) 106, 205, 433
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 4 cut(s) 69, 168, 384, 399
LmnI GCTCC 1 cut(s) 149
LpnPI CCDG 7 cut(s) 8, 35, 51, 95, 138, 388, 453
Lsp1109I GCAGC 1 cut(s) 59
LweI GCATC 2 cut(s) 34, 132
MaeI CTAG 1 cut(s) 33
MaeIII GTNAC 1 cut(s) 374
MalI GATC 4 cut(s) 71, 170, 386, 401
MboI GATC 4 cut(s) 69, 168, 384, 399
MboII GAAGA 2 cut(s) 407, 448
MfeI CAATTG 1 cut(s) 327
MluCI AATT 4 cut(s) 13, 172, 289, 327
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 9 cut(s) 98, 117, 130, 140, 175, 298, 326, 385, 464
MseI TTAA 3 cut(s) 117, 207, 288
MspR9I CCNGG 2 cut(s) 23, 468
MunI CAATTG 1 cut(s) 327
MvaI CCWGG 2 cut(s) 23, 468
NdeII GATC 4 cut(s) 69, 168, 384, 399
NlaIII CATG 3 cut(s) 106, 205, 433
NlaIV GGNNCC 1 cut(s) 157
NmuCI GTSAC 1 cut(s) 374
NspI RCATGY 1 cut(s) 433
NspV TTCGAA 1 cut(s) 459
PceI AGGCCT 1 cut(s) 65
PkrI GCNGC 1 cut(s) 49
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PshBI ATTAAT 1 cut(s) 288
Psp6I CCWGG 2 cut(s) 21, 466
PspGI CCWGG 2 cut(s) 21, 466
PspN4I GGNNCC 1 cut(s) 157
RsaI GTAC 5 cut(s) 187, 200, 251, 362, 434
RsaNI GTAC 5 cut(s) 186, 199, 250, 361, 433
SaqAI TTAA 3 cut(s) 117, 207, 288
SatI GCNGC 1 cut(s) 48
Sau3AI GATC 4 cut(s) 69, 168, 384, 399
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 2 cut(s) 23, 468
SetI ASST 8 cut(s) 90, 154, 224, 309, 322, 370, 451, 456
SfaNI GCATC 2 cut(s) 34, 132
SfuI TTCGAA 1 cut(s) 459
SpeI ACTAGT 1 cut(s) 32
Sse9I AATT 4 cut(s) 13, 172, 289, 327
SseBI AGGCCT 1 cut(s) 65
SspMI CTAG 1 cut(s) 33
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 2 cut(s) 21, 466
StyI CCWWGG 1 cut(s) 222
TaqI TCGA 3 cut(s) 75, 126, 459
TasI AATT 4 cut(s) 13, 172, 289, 327
TatI WGTACW 3 cut(s) 198, 249, 432
Tru1I TTAA 3 cut(s) 117, 207, 288
Tru9I TTAA 3 cut(s) 117, 207, 288
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 1 cut(s) 47
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 1 cut(s) 304
TspGWI ACGGA 1 cut(s) 159
VspI ATTAAT 1 cut(s) 288
XceI RCATGY 1 cut(s) 433
XspI CTAG 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.