Rmu_sc0003369.1_g000002

Major allergen Pru ar 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003369.1
OG0009655
Physical Location & Seq
Forward (+)
3067 .. 5340
2274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003369.1_g000002.1.cds

Sequence Viewer

Length: 708 bp
atggatcccattcgcaccagagatctggaaataccgatcgaggaaactgttgttttgaagatttccaacgacccttcgtcgaaaattggggccaccgtgacggaggcatcaaggcggcgtcagtggcgccggcttagaagtcgacgggcgtggtgcgcagaatggaatggtaggctgtggtggcatgggagagggaggctggaagagagcagaggagaggaggagtggattcatgatgagtacacttccccaatccctccatgcaggttgttcaaggcattggtccttgaggctgacatcctcgttccaaagctcatgccggaggctcttaagagcatcgacaccatccaaggtgacggaagagcaggaagcatcaagcagatcaacttcgccgaaggcatgcatataaaccagatcatttctgtgcgaaatcgggttgatgaggtggatgacgagaactacgtgtataattacactttgattgatggagatatttcagtaatggagaaacttgagtttatttcttatgaagttaagtttgaggctacaccagaaggtggcagtaagaacaagatggtgagcaagtaccacaccaaaggtgacattgtgctcaatgaggaagacataaaggccggcagagaaaaggccttggggatgtacaaagttgtggaagcctacctcctccaaaacccagatatctatacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

235

Amino Acids

27.13

Weight (kDa)

5.44

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 157
Acc36I ACCTGC 1 cut(s) 255
AccB1I GGYRCC 1 cut(s) 126
AccB7I CCANNNNNTGG 1 cut(s) 557
AccI GTMKAC 1 cut(s) 142
AciI CCGC 1 cut(s) 115
AclWI GGATC 1 cut(s) 12
AcyI GRCGYC 2 cut(s) 118, 127
AfaI GTAC 3 cut(s) 242, 587, 659
AfiI CCNNNNNNNGG 1 cut(s) 557
AflII CTTAAG 1 cut(s) 329
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 2 cut(s) 58, 274
AhdI GACNNNNNGTC 1 cut(s) 76
AluBI AGCT 1 cut(s) 313
AluI AGCT 1 cut(s) 313
Alw21I GWGCWC 1 cut(s) 612
AlwI GGATC 1 cut(s) 12
AoxI GGCC 3 cut(s) 90, 630, 645
AspLEI GCGC 2 cut(s) 129, 158
AspS9I GGNCC 2 cut(s) 90, 283
AsuHPI GGTGA 3 cut(s) 365, 589, 611
AvaII GGWCC 1 cut(s) 283
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 627
Bbv12I GWGCWC 1 cut(s) 612
BccI CCATC 3 cut(s) 353, 479, 568
BfoI RGCGCY 1 cut(s) 130
BfrI CTTAAG 1 cut(s) 329
BfuAI ACCTGC 1 cut(s) 255
BglII AGATCT 1 cut(s) 22
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
Bme18I GGWCC 1 cut(s) 283
BmeRI GACNNNNNGTC 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 90, 283
BmiI GGNNCC 3 cut(s) 6, 91, 128
BmsI GCATC 3 cut(s) 116, 345, 381
BpiI GAAGAC 1 cut(s) 627
BpuEI CTTGAG 2 cut(s) 308, 533
BsaAI YACGTR 1 cut(s) 463
BsaHI GRCGYC 2 cut(s) 118, 127
BsaJI CCNNGG 2 cut(s) 349, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 557
Bse118I RCCGGY 2 cut(s) 129, 632
BseDI CCNNGG 2 cut(s) 349, 648
BseGI GGATG 4 cut(s) 297, 345, 454, 660
BseLI CCNNNNNNNGG 1 cut(s) 557
BseRI GAGGAG 4 cut(s) 228, 233, 236, 671
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 3 cut(s) 92, 632, 647
BshNI GGYRCC 1 cut(s) 126
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 3 cut(s) 130, 320, 633
BslI CCNNNNNNNGG 1 cut(s) 557
BsnI GGCC 3 cut(s) 92, 632, 647
Bsp1286I GDGCHC 1 cut(s) 612
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 5 cut(s) 4, 22, 36, 381, 414
BspACI CCGC 1 cut(s) 115
BspANI GGCC 3 cut(s) 92, 632, 647
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 3 cut(s) 6, 91, 128
BspMI ACCTGC 1 cut(s) 255
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 355
BspT107I GGYRCC 1 cut(s) 126
BspTI CTTAAG 1 cut(s) 329
BsrFI RCCGGY 2 cut(s) 129, 632
BsrGI TGTACA 1 cut(s) 657
BssAI RCCGGY 2 cut(s) 129, 632
BssECI CCNNGG 2 cut(s) 349, 648
BssMI GATC 5 cut(s) 4, 22, 36, 381, 414
BssNI GRCGYC 2 cut(s) 118, 127
BssT1I CCWWGG 2 cut(s) 349, 648
Bst4CI ACNGT 2 cut(s) 49, 97
Bst6I CTCTTC 2 cut(s) 198, 355
BstACI GRCGYC 2 cut(s) 118, 127
BstAFI CTTAAG 1 cut(s) 329
BstAUI TGTACA 1 cut(s) 657
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 3 cut(s) 131, 401, 634
BstDEI CTNAG 1 cut(s) 134
BstF5I GGATG 4 cut(s) 297, 345, 454, 660
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 2 cut(s) 129, 158
BstKTI GATC 5 cut(s) 7, 25, 39, 384, 417
BstMBI GATC 5 cut(s) 4, 22, 36, 381, 414
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 3 cut(s) 124, 155, 181
BstNSI RCATGY 1 cut(s) 403
BstV2I GAAGAC 1 cut(s) 627
BstX2I RGATCY 2 cut(s) 4, 22
BstXI CCANNNNNNTGG 1 cut(s) 25
BstYI RGATCY 2 cut(s) 4, 22
BsuRI GGCC 3 cut(s) 92, 632, 647
BtsCI GGATG 4 cut(s) 297, 345, 454, 660
BtsIMutI CAGTG 1 cut(s) 128
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 3 cut(s) 131, 401, 634
CciI TCATGA 1 cut(s) 232
CfoI GCGC 2 cut(s) 129, 158
Cfr10I RCCGGY 2 cut(s) 129, 632
Cfr13I GGNCC 2 cut(s) 90, 283
CseI GACGC 1 cut(s) 107
Csp6I GTAC 3 cut(s) 241, 586, 658
CviAII CATG 5 cut(s) 185, 233, 261, 316, 400
CviQI GTAC 3 cut(s) 241, 586, 658
DdeI CTNAG 1 cut(s) 134
DinI GGCGCC 1 cut(s) 128
DpnI GATC 5 cut(s) 6, 24, 38, 383, 416
DpnII GATC 5 cut(s) 4, 22, 36, 381, 414
DriI GACNNNNNGTC 1 cut(s) 76
Eam1104I CTCTTC 2 cut(s) 198, 355
Eam1105I GACNNNNNGTC 1 cut(s) 76
EarI CTCTTC 2 cut(s) 198, 355
Eco130I CCWWGG 2 cut(s) 349, 648
Eco147I AGGCCT 1 cut(s) 647
Eco32I GATATC 1 cut(s) 697
Eco47I GGWCC 1 cut(s) 283
EcoRV GATATC 1 cut(s) 697
EcoT14I CCWWGG 2 cut(s) 349, 648
EcoT22I ATGCAT 1 cut(s) 405
EgeI GGCGCC 1 cut(s) 128
EheI GGCGCC 1 cut(s) 128
ErhI CCWWGG 2 cut(s) 349, 648
FaeI CATG 5 cut(s) 188, 236, 264, 319, 403
FatI CATG 5 cut(s) 184, 232, 260, 315, 399
FblI GTMKAC 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 4 cut(s) 284, 332, 461, 667
Fsp4HI GCNGC 1 cut(s) 116
FspI TGCGCA 1 cut(s) 157
GlaI GCGC 2 cut(s) 128, 157
GluI GCNGC 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 3 cut(s) 92, 632, 647
HapII CCGG 3 cut(s) 130, 320, 633
HgaI GACGC 1 cut(s) 107
HhaI GCGC 2 cut(s) 129, 158
Hin1I GRCGYC 2 cut(s) 118, 127
Hin1II CATG 5 cut(s) 188, 236, 264, 319, 403
Hin6I GCGC 2 cut(s) 127, 156
HinP1I GCGC 2 cut(s) 127, 156
HincII GTYRAC 1 cut(s) 143
HindII GTYRAC 1 cut(s) 143
HinfI GANTC 1 cut(s) 229
HpaII CCGG 3 cut(s) 130, 320, 633
HphI GGTGA 3 cut(s) 365, 589, 611
Hpy166II GTNNAC 2 cut(s) 143, 243
Hpy188III TCNNGA 2 cut(s) 26, 233
Hpy8I GTNNAC 2 cut(s) 143, 243
Hpy99I CGWCG 2 cut(s) 82, 147
HpyAV CCTTC 3 cut(s) 84, 389, 548
HpyCH4III ACNGT 2 cut(s) 49, 97
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 2 cut(s) 264, 403
HpyF10VI GCNNNNNNNGC 3 cut(s) 124, 155, 181
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 1 cut(s) 462
Hsp92I GRCGYC 2 cut(s) 118, 127
Hsp92II CATG 5 cut(s) 188, 236, 264, 319, 403
HspAI GCGC 2 cut(s) 127, 156
KasI GGCGCC 1 cut(s) 126
KroI GCCGGC 2 cut(s) 129, 632
KroNI GCCGGC 2 cut(s) 131, 634
Kzo9I GATC 5 cut(s) 4, 22, 36, 381, 414
LguI GCTCTTC 1 cut(s) 355
LweI GCATC 3 cut(s) 116, 345, 381
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 3 cut(s) 97, 353, 599
MalI GATC 5 cut(s) 6, 24, 38, 383, 416
MboI GATC 5 cut(s) 4, 22, 36, 381, 414
MboII GAAGA 4 cut(s) 70, 215, 372, 632
MflI RGATCY 2 cut(s) 4, 22
MhlI GDGCHC 1 cut(s) 612
MluCI AATT 2 cut(s) 84, 469
Mly113I GGCGCC 1 cut(s) 127
MmeI TCCRAC 1 cut(s) 90
Mph1103I ATGCAT 1 cut(s) 405
MroNI GCCGGC 2 cut(s) 129, 632
MseI TTAA 2 cut(s) 330, 534
MslI CAYNNNNRTG 1 cut(s) 422
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 3 cut(s) 130, 320, 633
MwoI GCNNNNNNNGC 3 cut(s) 124, 155, 181
NaeI GCCGGC 2 cut(s) 131, 634
NarI GGCGCC 1 cut(s) 127
NdeII GATC 5 cut(s) 4, 22, 36, 381, 414
NgoMIV GCCGGC 2 cut(s) 129, 632
NlaIII CATG 5 cut(s) 188, 236, 264, 319, 403
NlaIV GGNNCC 3 cut(s) 6, 91, 128
NmuCI GTSAC 3 cut(s) 97, 353, 599
NsbI TGCGCA 1 cut(s) 157
NsiI ATGCAT 1 cut(s) 405
NspI RCATGY 1 cut(s) 403
PaeI GCATGC 1 cut(s) 403
PagI TCATGA 1 cut(s) 232
PceI AGGCCT 1 cut(s) 647
PciSI GCTCTTC 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PdiI GCCGGC 2 cut(s) 131, 634
PfeI GAWTC 1 cut(s) 229
PflMI CCANNNNNTGG 1 cut(s) 557
PkrI GCNGC 1 cut(s) 117
Ple19I CGATCG 1 cut(s) 39
PluTI GGCGCC 1 cut(s) 130
Ppu21I YACGTR 1 cut(s) 463
PspN4I GGNNCC 3 cut(s) 6, 91, 128
PspPI GGNCC 2 cut(s) 90, 283
PsuI RGATCY 2 cut(s) 4, 22
PvuI CGATCG 1 cut(s) 39
RsaI GTAC 3 cut(s) 242, 587, 659
RsaNI GTAC 3 cut(s) 241, 586, 658
RseI CAYNNNNRTG 1 cut(s) 422
SalI GTCGAC 1 cut(s) 141
SapI GCTCTTC 1 cut(s) 355
SaqAI TTAA 2 cut(s) 330, 534
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 5 cut(s) 4, 22, 36, 381, 414
Sau96I GGNCC 2 cut(s) 90, 283
SduI GDGCHC 1 cut(s) 612
SetI ASST 8 cut(s) 269, 315, 355, 447, 465, 559, 601, 681
SfaNI GCATC 3 cut(s) 116, 345, 381
SfoI GGCGCC 1 cut(s) 128
SinI GGWCC 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 3 cut(s) 287, 329, 512
SmoI CTYRAG 3 cut(s) 287, 329, 512
SphI GCATGC 1 cut(s) 403
Sse9I AATT 2 cut(s) 84, 469
SseBI AGGCCT 1 cut(s) 647
SsiI CCGC 1 cut(s) 115
SspDI GGCGCC 1 cut(s) 126
StuI AGGCCT 1 cut(s) 647
StyI CCWWGG 2 cut(s) 349, 648
TaaI ACNGT 2 cut(s) 49, 97
TaiI ACGT 1 cut(s) 465
TaqI TCGA 4 cut(s) 39, 80, 142, 339
TasI AATT 2 cut(s) 84, 469
TatI WGTACW 2 cut(s) 240, 657
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 1 cut(s) 229
Tru1I TTAA 2 cut(s) 330, 534
Tru9I TTAA 2 cut(s) 330, 534
TscAI CASTG 1 cut(s) 128
TseFI GTSAC 3 cut(s) 97, 353, 599
Tsp45I GTSAC 3 cut(s) 97, 353, 599
TspDTI ATGAA 2 cut(s) 221, 543
TspGWI ACGGA 2 cut(s) 116, 372
TspRI CASTG 1 cut(s) 128
Van91I CCANNNNNTGG 1 cut(s) 557
Vha464I CTTAAG 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 283
XceI RCATGY 1 cut(s) 403
XmiI GTMKAC 1 cut(s) 142
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.