Rw4G021500

Major allergen Pru ar 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009655
Physical Location & Seq
Reverse (-)
46176484 .. 46177332
849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G021500.1

Sequence Viewer

Length: 441 bp
ATGGGGGTGACCACCTATACAGATGAGTACACTTCCCCAATCCCTCCATGCAGGTTGTTCAAGGCATTGGTCCTTGAGGCTGACATCCTCGTTCCAAAGCTCATGCCAGAGGCTCTTAAGAGCATCGACACCATCCAAGGCAGCAAGATCATTTCTGTGCGAAATCGGGTTGATGAGGTGGATGACGAGAACTACGTGTATAATTACACTTTGATTGATGGAGATATTTCAGTAATGGAGAAACTTGAGTTTATTTCTTATGAAGTTAAGTTTGAGGCTACACCAGAAGGTGGCAGTAAGAACAAGATGGTGAGCAAGTACCACACCAAAGGTGACATTGTGCTCAATGAGGAAGACATAAAGGCCGGCAGAGAAAAGGCCTTGGGGATGTACAAAGTTGTGGAAGCCTACCTCCTCCAAAACCCAGATATATATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

146

Amino Acids

16.54

Weight (kDa)

4.71

Isoelectric Point (pI)

20.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 5 - 141 3.8e-09 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 42
AccB7I CCANNNNNTGG 1 cut(s) 290
AfaI GTAC 3 cut(s) 29, 320, 392
AfiI CCNNNNNNNGG 1 cut(s) 290
AflII CTTAAG 1 cut(s) 116
AflIII ACRYGT 1 cut(s) 195
AgsI TTSAA 1 cut(s) 61
AluBI AGCT 1 cut(s) 100
AluI AGCT 1 cut(s) 100
Alw21I GWGCWC 1 cut(s) 345
AoxI GGCC 2 cut(s) 363, 378
ApeKI GCWGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 70
AsuHPI GGTGA 3 cut(s) 19, 322, 344
AvaII GGWCC 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 360
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 1 cut(s) 153
BccI CCATC 3 cut(s) 140, 212, 301
BfrI CTTAAG 1 cut(s) 116
BfuAI ACCTGC 1 cut(s) 42
BisI GCNGC 1 cut(s) 142
BlsI GCNGC 1 cut(s) 143
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 70
BmsI GCATC 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 360
BpuEI CTTGAG 2 cut(s) 95, 266
BsaAI YACGTR 1 cut(s) 196
BsaJI CCNNGG 2 cut(s) 136, 381
Bsc4I CCNNNNNNNGG 1 cut(s) 290
Bse118I RCCGGY 1 cut(s) 365
BseDI CCNNGG 2 cut(s) 136, 381
BseGI GGATG 4 cut(s) 84, 132, 187, 393
BseLI CCNNNNNNNGG 1 cut(s) 290
BseRI GAGGAG 1 cut(s) 404
BseXI GCAGC 1 cut(s) 153
BshFI GGCC 2 cut(s) 365, 380
BsiHKAI GWGCWC 1 cut(s) 345
BsiSI CCGG 1 cut(s) 366
BslI CCNNNNNNNGG 1 cut(s) 290
BsnI GGCC 2 cut(s) 365, 380
Bsp1286I GDGCHC 1 cut(s) 345
Bsp1407I TGTACA 1 cut(s) 390
Bsp143I GATC 1 cut(s) 147
BspANI GGCC 2 cut(s) 365, 380
BspMI ACCTGC 1 cut(s) 42
BspTI CTTAAG 1 cut(s) 116
BsrFI RCCGGY 1 cut(s) 365
BsrGI TGTACA 1 cut(s) 390
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 2 cut(s) 136, 381
BssMI GATC 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 136, 381
BstAFI CTTAAG 1 cut(s) 116
BstAUI TGTACA 1 cut(s) 390
BstBAI YACGTR 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 367
BstEII GGTNACC 1 cut(s) 7
BstF5I GGATG 4 cut(s) 84, 132, 187, 393
BstKTI GATC 1 cut(s) 150
BstMBI GATC 1 cut(s) 147
BstPI GGTNACC 1 cut(s) 7
BstV1I GCAGC 1 cut(s) 153
BstV2I GAAGAC 1 cut(s) 360
BsuRI GGCC 2 cut(s) 365, 380
BtsCI GGATG 4 cut(s) 84, 132, 187, 393
BveI ACCTGC 1 cut(s) 42
Cac8I GCNNGC 1 cut(s) 367
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 70
Csp6I GTAC 3 cut(s) 28, 319, 391
CviAII CATG 2 cut(s) 48, 103
CviJI RGCY 7 cut(s) 80, 100, 113, 278, 365, 380, 407
CviKI_1 RGCY 7 cut(s) 80, 100, 113, 278, 365, 380, 407
CviQI GTAC 3 cut(s) 28, 319, 391
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
Eco130I CCWWGG 2 cut(s) 136, 381
Eco147I AGGCCT 1 cut(s) 380
Eco47I GGWCC 1 cut(s) 70
Eco91I GGTNACC 1 cut(s) 7
EcoO65I GGTNACC 1 cut(s) 7
EcoT14I CCWWGG 2 cut(s) 136, 381
ErhI CCWWGG 2 cut(s) 136, 381
FaeI CATG 2 cut(s) 51, 106
FaiI YATR 9 cut(s) 18, 49, 104, 201, 261, 359, 431, 433, 435
FatI CATG 2 cut(s) 47, 102
Fnu4HI GCNGC 1 cut(s) 142
FokI GGATG 4 cut(s) 71, 119, 194, 400
Fsp4HI GCNGC 1 cut(s) 142
GluI GCNGC 1 cut(s) 142
HaeIII GGCC 2 cut(s) 365, 380
HapII CCGG 1 cut(s) 366
Hin1II CATG 2 cut(s) 51, 106
HpaII CCGG 1 cut(s) 366
HphI GGTGA 3 cut(s) 19, 322, 344
Hpy166II GTNNAC 1 cut(s) 30
Hpy8I GTNNAC 1 cut(s) 30
HpyAV CCTTC 1 cut(s) 281
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 1 cut(s) 51
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 2 cut(s) 51, 106
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Kzo9I GATC 1 cut(s) 147
LpnPI CCDG 4 cut(s) 37, 120, 297, 379
Lsp1109I GCAGC 1 cut(s) 153
LweI GCATC 1 cut(s) 132
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 2 cut(s) 7, 332
MalI GATC 1 cut(s) 149
MboI GATC 1 cut(s) 147
MboII GAAGA 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 345
MluCI AATT 1 cut(s) 202
MnlI CCTC 9 cut(s) 54, 70, 98, 103, 169, 268, 343, 422, 425
MroNI GCCGGC 1 cut(s) 365
MseI TTAA 2 cut(s) 117, 267
MslI CAYNNNNRTG 1 cut(s) 155
MspCI CTTAAG 1 cut(s) 116
MspI CCGG 1 cut(s) 366
NaeI GCCGGC 1 cut(s) 367
NdeII GATC 1 cut(s) 147
NgoMIV GCCGGC 1 cut(s) 365
NlaIII CATG 2 cut(s) 51, 106
NmuCI GTSAC 2 cut(s) 7, 332
PceI AGGCCT 1 cut(s) 380
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PdiI GCCGGC 1 cut(s) 367
PflMI CCANNNNNTGG 1 cut(s) 290
PkrI GCNGC 1 cut(s) 143
Ppu21I YACGTR 1 cut(s) 196
PspEI GGTNACC 1 cut(s) 7
PspPI GGNCC 1 cut(s) 70
RsaI GTAC 3 cut(s) 29, 320, 392
RsaNI GTAC 3 cut(s) 28, 319, 391
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 2 cut(s) 117, 267
SatI GCNGC 1 cut(s) 142
Sau3AI GATC 1 cut(s) 147
Sau96I GGNCC 1 cut(s) 70
SduI GDGCHC 1 cut(s) 345
SetI ASST 8 cut(s) 17, 56, 102, 180, 198, 292, 334, 414
SfaNI GCATC 1 cut(s) 132
SinI GGWCC 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 3 cut(s) 74, 116, 245
SmoI CTYRAG 3 cut(s) 74, 116, 245
Sse9I AATT 1 cut(s) 202
SseBI AGGCCT 1 cut(s) 380
StuI AGGCCT 1 cut(s) 380
StyI CCWWGG 2 cut(s) 136, 381
TaiI ACGT 1 cut(s) 198
TaqI TCGA 1 cut(s) 126
TasI AATT 1 cut(s) 202
TatI WGTACW 2 cut(s) 27, 390
Tru1I TTAA 2 cut(s) 117, 267
Tru9I TTAA 2 cut(s) 117, 267
TseFI GTSAC 2 cut(s) 7, 332
TseI GCWGC 1 cut(s) 141
Tsp45I GTSAC 2 cut(s) 7, 332
TspDTI ATGAA 1 cut(s) 276
Van91I CCANNNNNTGG 1 cut(s) 290
Vha464I CTTAAG 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.