RLG00000011861

Major allergen Pru ar

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009655
Physical Location & Seq
Forward (+)
13388774 .. 13389346
573 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011861

Sequence Viewer

Length: 483 bp
ATGGGTGTCATCAATTTGAGCCAGGAGTTTACTAGTCCAGTAGATGCAGCAAGATTGTTCAAGGCCTTGATCATCGACTCCCACAACCTCATCCCAAGACTCATGCCTCAGGCCATTAAGAGCATCGAAATCATTCAGGGTGATGGAGGAGCAGGAACCATCAAACAGATCAATTTTGCTGAGGGTACTCGTTTCAAGTACATGAAGAACAGAATAGACACCCTGGATGTAGAAAACTTGATGTGCAGGTACACGCTGATTGAAGGCGATGTATTGGAGGACAAGATTAAGTCTATTGCTTATGAGGTGAGGTTTGTACCTTCTGCAAATGGAGGATGTGTTTGTAAGATGATGAGTGAGTACCAAGCTGTTGGTGACTTTGAGATCAATGAGGAAGAGATCAGGGGTGGCAAGGAAAGAGCAATGGGAATGTACAAAGTAGTTGAAGCTTACCTCTTCGAAAGTCCTGGTGCTTACGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

161

Amino Acids

17.92

Weight (kDa)

4.95

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 153 2.8e-25 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 237
AfaI GTAC 6 cut(s) 187, 200, 251, 318, 362, 434
AgsI TTSAA 4 cut(s) 61, 196, 263, 446
AhlI ACTAGT 1 cut(s) 32
AjnI CCWGG 3 cut(s) 21, 222, 466
AluBI AGCT 2 cut(s) 368, 449
AluI AGCT 2 cut(s) 368, 449
AoxI GGCC 2 cut(s) 63, 111
ApeKI GCWGC 1 cut(s) 47
Asp700I GAANNNNTTC 1 cut(s) 132
AsuHPI GGTGA 3 cut(s) 152, 319, 386
AsuII TTCGAA 1 cut(s) 459
AxyI CCTNAGG 1 cut(s) 108
BbvCI CCTCAGC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 59
BccI CCATC 2 cut(s) 137, 167
BciT130I CCWGG 3 cut(s) 23, 224, 468
BclI TGATCA 1 cut(s) 69
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 237
BisI GCNGC 1 cut(s) 48
BlsI GCNGC 1 cut(s) 49
Bme1390I CCNGG 3 cut(s) 23, 224, 468
BmiI GGNNCC 1 cut(s) 157
BmrFI CCNGG 3 cut(s) 23, 224, 468
BmsI GCATC 2 cut(s) 34, 132
Bpu10I CCTNAGC 1 cut(s) 180
Bpu14I TTCGAA 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 222
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bse1I ACTGG 1 cut(s) 38
Bse21I CCTNAGG 1 cut(s) 108
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 3 cut(s) 23, 224, 468
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 3 cut(s) 90, 232, 341
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 2 cut(s) 122, 171
BseNI ACTGG 1 cut(s) 38
BseRI GAGGAG 1 cut(s) 162
BseXI GCAGC 1 cut(s) 59
BsgI GTGCAG 1 cut(s) 265
BshFI GGCC 2 cut(s) 65, 113
BsnI GGCC 2 cut(s) 65, 113
Bsp119I TTCGAA 1 cut(s) 459
Bsp1407I TGTACA 1 cut(s) 432
Bsp143I GATC 4 cut(s) 69, 168, 384, 399
BspANI GGCC 2 cut(s) 65, 113
BspCNI CTCAG 2 cut(s) 121, 172
BspLI GGNNCC 1 cut(s) 157
BspMI ACCTGC 1 cut(s) 237
BspT104I TTCGAA 1 cut(s) 459
BsrDI GCAATG 1 cut(s) 429
BsrGI TGTACA 1 cut(s) 432
BsrI ACTGG 1 cut(s) 38
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 4 cut(s) 69, 168, 384, 399
Bst2UI CCWGG 3 cut(s) 23, 224, 468
Bst6I CTCTTC 2 cut(s) 390, 461
BstAUI TGTACA 1 cut(s) 432
BstBI TTCGAA 1 cut(s) 459
BstDEI CTNAG 2 cut(s) 108, 180
BstF5I GGATG 3 cut(s) 90, 232, 341
BstKTI GATC 4 cut(s) 72, 171, 387, 402
BstMBI GATC 4 cut(s) 69, 168, 384, 399
BstNI CCWGG 3 cut(s) 23, 224, 468
BstSCI CCNGG 3 cut(s) 21, 222, 466
BstV1I GCAGC 1 cut(s) 59
BstXI CCANNNNNNTGG 1 cut(s) 371
Bsu36I CCTNAGG 1 cut(s) 108
BsuRI GGCC 2 cut(s) 65, 113
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 3 cut(s) 90, 232, 341
BveI ACCTGC 1 cut(s) 237
Csp6I GTAC 6 cut(s) 186, 199, 250, 317, 361, 433
CviAII CATG 2 cut(s) 103, 202
CviJI RGCY 5 cut(s) 21, 65, 113, 368, 449
CviKI_1 RGCY 5 cut(s) 21, 65, 113, 368, 449
CviQI GTAC 6 cut(s) 186, 199, 250, 317, 361, 433
DdeI CTNAG 2 cut(s) 108, 180
DpnI GATC 4 cut(s) 71, 170, 386, 401
DpnII GATC 4 cut(s) 69, 168, 384, 399
Eam1104I CTCTTC 2 cut(s) 390, 461
EarI CTCTTC 2 cut(s) 390, 461
Eco147I AGGCCT 1 cut(s) 65
Eco81I CCTNAGG 1 cut(s) 108
EcoRII CCWGG 3 cut(s) 21, 222, 466
FaeI CATG 2 cut(s) 106, 205
FaiI YATR 3 cut(s) 104, 203, 303
FatI CATG 2 cut(s) 102, 201
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 48
FokI GGATG 3 cut(s) 77, 239, 348
Fsp4HI GCNGC 1 cut(s) 48
FspBI CTAG 1 cut(s) 33
GluI GCNGC 1 cut(s) 48
HaeIII GGCC 2 cut(s) 65, 113
Hin1II CATG 2 cut(s) 106, 205
HindIII AAGCTT 1 cut(s) 447
HinfI GANTC 2 cut(s) 77, 99
HphI GGTGA 3 cut(s) 152, 319, 386
Hpy166II GTNNAC 2 cut(s) 30, 252
Hpy8I GTNNAC 2 cut(s) 30, 252
HpyAV CCTTC 2 cut(s) 257, 330
HpyCH4V TGCA 3 cut(s) 47, 246, 326
HpyF3I CTNAG 2 cut(s) 108, 180
Hsp92II CATG 2 cut(s) 106, 205
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 4 cut(s) 69, 168, 384, 399
LmnI GCTCC 1 cut(s) 149
Lsp1109I GCAGC 1 cut(s) 59
LweI GCATC 2 cut(s) 34, 132
MaeI CTAG 1 cut(s) 33
MaeIII GTNAC 1 cut(s) 374
MalI GATC 4 cut(s) 71, 170, 386, 401
MboI GATC 4 cut(s) 69, 168, 384, 399
MboII GAAGA 3 cut(s) 217, 407, 448
MluCI AATT 2 cut(s) 13, 172
MlyI GAGTC 2 cut(s) 71, 93
MroXI GAANNNNTTC 1 cut(s) 132
MseI TTAA 2 cut(s) 117, 288
MspR9I CCNGG 3 cut(s) 23, 224, 468
MvaI CCWGG 3 cut(s) 23, 224, 468
NdeII GATC 4 cut(s) 69, 168, 384, 399
NlaIII CATG 2 cut(s) 106, 205
NlaIV GGNNCC 1 cut(s) 157
NmuCI GTSAC 1 cut(s) 374
NspV TTCGAA 1 cut(s) 459
PceI AGGCCT 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 132
PkrI GCNGC 1 cut(s) 49
PleI GAGTC 2 cut(s) 71, 93
PpsI GAGTC 2 cut(s) 71, 93
Psp6I CCWGG 3 cut(s) 21, 222, 466
PspGI CCWGG 3 cut(s) 21, 222, 466
PspN4I GGNNCC 1 cut(s) 157
RsaI GTAC 6 cut(s) 187, 200, 251, 318, 362, 434
RsaNI GTAC 6 cut(s) 186, 199, 250, 317, 361, 433
SaqAI TTAA 2 cut(s) 117, 288
SatI GCNGC 1 cut(s) 48
Sau3AI GATC 4 cut(s) 69, 168, 384, 399
SchI GAGTC 2 cut(s) 71, 93
ScrFI CCNGG 3 cut(s) 23, 224, 468
SetI ASST 8 cut(s) 90, 251, 309, 314, 322, 370, 451, 456
SfaNI GCATC 2 cut(s) 34, 132
SfuI TTCGAA 1 cut(s) 459
SpeI ACTAGT 1 cut(s) 32
Sse9I AATT 2 cut(s) 13, 172
SseBI AGGCCT 1 cut(s) 65
SspMI CTAG 1 cut(s) 33
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 3 cut(s) 21, 222, 466
TaqI TCGA 3 cut(s) 75, 126, 459
TasI AATT 2 cut(s) 13, 172
TatI WGTACW 2 cut(s) 198, 432
Tru1I TTAA 2 cut(s) 117, 288
Tru9I TTAA 2 cut(s) 117, 288
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 1 cut(s) 47
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 1 cut(s) 218
XmnI GAANNNNTTC 1 cut(s) 132
XspI CTAG 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.