MD16G1160800.v1.1

Major allergen Pru ar 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009655
Physical Location & Seq
Forward (+)
13114991 .. 13115874
884 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1160800.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGGTGTCTTCACTCGTACAGATGAGTATACTTCCCCAATCCCTCCGGACAGGTTGTTCAAGGCCTTGGTCCTTGATGCTCACATCCTGATCCCAGAGCTCATGCCAGAGGCTGTTAAGAGCATTGACACCCTCGAAGGTGACGGAAGAGCAGGAAGCATCAAGAAGATCAACTTCGCTGAAGGCATCCAGCTCAAATCTGTGATAAACAGGGTTGACGAAGTCGACGAAGAGAACTTTGTATATGCTTACACTTTGGTTGAAGGAGAACCATTAGTAGTGGAGAAACTTGAATACATCACTTATAAGGCTAAGTTTGAAGCTGCATCAGATGGGGGTAGTAAGAACCGGTTGGTCAGCAATTACTACACCAAGGGTGACATTGTGCTGAAAGAGGAAGAAATAAAAGCTGGCAGGGAAAAGGCCTTGGGGATGTACAGAGTTGTGGAAACCTATCTCCTCCAAAATCCTGATGCTTATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

162

Amino Acids

18.07

Weight (kDa)

4.8

Isoelectric Point (pI)

22.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 155 1.6e-16 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 306
AccI GTMKAC 2 cut(s) 29, 225
AccIII TCCGGA 1 cut(s) 46
AclWI GGATC 1 cut(s) 85
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 2 cut(s) 19, 437
AgeI ACCGGT 1 cut(s) 348
AgsI TTSAA 4 cut(s) 61, 263, 293, 320
AloI GAACNNNNNNTCC 2 cut(s) 41, 73
AluBI AGCT 4 cut(s) 100, 193, 323, 410
AluI AGCT 4 cut(s) 100, 193, 323, 410
Alw21I GWGCWC 1 cut(s) 102
AlwI GGATC 1 cut(s) 85
AlwNI CAGNNNCTG 1 cut(s) 113
Aor13HI TCCGGA 1 cut(s) 46
AoxI GGCC 2 cut(s) 63, 423
ApeKI GCWGC 1 cut(s) 323
AsiGI ACCGGT 1 cut(s) 348
AspS9I GGNCC 1 cut(s) 70
AsuHPI GGTGA 2 cut(s) 152, 389
AvaII GGWCC 1 cut(s) 70
BanII GRGCYC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 310
BccI CCATC 1 cut(s) 326
BisI GCNGC 1 cut(s) 324
BlsI GCNGC 1 cut(s) 325
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 70
BmsI GCATC 5 cut(s) 67, 168, 195, 335, 463
BsaJI CCNNGG 3 cut(s) 66, 372, 426
BsaWI WCCGGW 2 cut(s) 46, 348
Bse118I RCCGGY 1 cut(s) 348
BseAI TCCGGA 1 cut(s) 46
BseDI CCNNGG 3 cut(s) 66, 372, 426
BseGI GGATG 3 cut(s) 84, 186, 438
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 1 cut(s) 310
BshFI GGCC 2 cut(s) 65, 425
BshTI ACCGGT 1 cut(s) 348
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 2 cut(s) 47, 349
BsnI GGCC 2 cut(s) 65, 425
Bsp1286I GDGCHC 1 cut(s) 102
Bsp13I TCCGGA 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 2 cut(s) 90, 168
BspANI GGCC 2 cut(s) 65, 425
BspEI TCCGGA 1 cut(s) 46
BspPI GGATC 1 cut(s) 85
BspQI GCTCTTC 1 cut(s) 142
BsrFI RCCGGY 1 cut(s) 348
BsrGI TGTACA 1 cut(s) 435
BssAI RCCGGY 1 cut(s) 348
BssECI CCNNGG 3 cut(s) 66, 372, 426
BssMI GATC 2 cut(s) 90, 168
BssNAI GTATAC 1 cut(s) 30
BssT1I CCWWGG 3 cut(s) 66, 372, 426
Bst1107I GTATAC 1 cut(s) 30
Bst6I CTCTTC 2 cut(s) 142, 225
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 1 cut(s) 312
BstF5I GGATG 3 cut(s) 84, 186, 438
BstKTI GATC 2 cut(s) 93, 171
BstMBI GATC 2 cut(s) 90, 168
BstV1I GCAGC 1 cut(s) 310
BstZ17I GTATAC 1 cut(s) 30
BsuRI GGCC 2 cut(s) 65, 425
BtsCI GGATG 3 cut(s) 84, 186, 438
Cac8I GCNNGC 1 cut(s) 412
CaiI CAGNNNCTG 1 cut(s) 113
Cfr10I RCCGGY 1 cut(s) 348
Cfr13I GGNCC 1 cut(s) 70
Csp6I GTAC 2 cut(s) 18, 436
CspAI ACCGGT 1 cut(s) 348
CviAII CATG 1 cut(s) 103
CviJI RGCY 8 cut(s) 65, 100, 113, 193, 311, 323, 410, 425
CviKI_1 RGCY 8 cut(s) 65, 100, 113, 193, 311, 323, 410, 425
CviQI GTAC 2 cut(s) 18, 436
DdeI CTNAG 1 cut(s) 312
DpnI GATC 2 cut(s) 92, 170
DpnII GATC 2 cut(s) 90, 168
Eam1104I CTCTTC 2 cut(s) 142, 225
EarI CTCTTC 2 cut(s) 142, 225
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 3 cut(s) 66, 372, 426
Eco147I AGGCCT 2 cut(s) 65, 425
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 70
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 201
EcoICRI GAGCTC 1 cut(s) 100
EcoT14I CCWWGG 3 cut(s) 66, 372, 426
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 3 cut(s) 66, 372, 426
FaeI CATG 1 cut(s) 106
FaiI YATR 6 cut(s) 30, 104, 244, 246, 306, 480
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FatI CATG 1 cut(s) 102
FblI GTMKAC 2 cut(s) 29, 225
Fnu4HI GCNGC 1 cut(s) 324
FokI GGATG 3 cut(s) 71, 173, 445
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 1 cut(s) 324
GluI GCNGC 1 cut(s) 324
HaeIII GGCC 2 cut(s) 65, 425
HapII CCGG 2 cut(s) 47, 349
Hin1II CATG 1 cut(s) 106
HincII GTYRAC 2 cut(s) 217, 226
HindII GTYRAC 2 cut(s) 217, 226
HpaII CCGG 2 cut(s) 47, 349
HphI GGTGA 2 cut(s) 152, 389
Hpy166II GTNNAC 3 cut(s) 30, 217, 226
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 4 cut(s) 47, 88, 163, 470
Hpy8I GTNNAC 3 cut(s) 30, 217, 226
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 3 cut(s) 131, 176, 257
HpyCH4V TGCA 1 cut(s) 326
HpyF3I CTNAG 1 cut(s) 312
Hsp92II CATG 1 cut(s) 106
Kpn2I TCCGGA 1 cut(s) 46
Kzo9I GATC 2 cut(s) 90, 168
LguI GCTCTTC 1 cut(s) 142
Lsp1109I GCAGC 1 cut(s) 310
LweI GCATC 5 cut(s) 67, 168, 195, 335, 463
MaeIII GTNAC 2 cut(s) 140, 377
MalI GATC 2 cut(s) 92, 170
MboI GATC 2 cut(s) 90, 168
MboII GAAGA 4 cut(s) 159, 178, 242, 410
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 1 cut(s) 361
MnlI CCTC 5 cut(s) 54, 103, 143, 388, 470
MroI TCCGGA 1 cut(s) 46
MseI TTAA 2 cut(s) 117, 484
MspI CCGG 2 cut(s) 47, 349
NdeII GATC 2 cut(s) 90, 168
NlaIII CATG 1 cut(s) 106
NmuCI GTSAC 2 cut(s) 140, 377
PceI AGGCCT 2 cut(s) 65, 425
PciSI GCTCTTC 1 cut(s) 142
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PflFI GACNNNGTC 1 cut(s) 221
PinAI ACCGGT 1 cut(s) 348
PkrI GCNGC 1 cut(s) 325
PsiI TTATAA 1 cut(s) 306
Psp124BI GAGCTC 1 cut(s) 102
PspPI GGNCC 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 113
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 2 cut(s) 19, 437
RsaNI GTAC 2 cut(s) 18, 436
SacI GAGCTC 1 cut(s) 102
SalI GTCGAC 1 cut(s) 224
SapI GCTCTTC 1 cut(s) 142
SaqAI TTAA 2 cut(s) 117, 484
SatI GCNGC 1 cut(s) 324
Sau3AI GATC 2 cut(s) 90, 168
Sau96I GGNCC 1 cut(s) 70
SduI GDGCHC 1 cut(s) 102
SetI ASST 7 cut(s) 56, 102, 142, 195, 325, 412, 455
SfaNI GCATC 5 cut(s) 67, 168, 195, 335, 463
SinI GGWCC 1 cut(s) 70
Sse9I AATT 1 cut(s) 361
SseBI AGGCCT 2 cut(s) 65, 425
SstI GAGCTC 1 cut(s) 102
StuI AGGCCT 2 cut(s) 65, 425
StyI CCWWGG 3 cut(s) 66, 372, 426
TaqI TCGA 2 cut(s) 135, 225
TasI AATT 1 cut(s) 361
TatI WGTACW 1 cut(s) 435
Tru1I TTAA 2 cut(s) 117, 484
Tru9I TTAA 2 cut(s) 117, 484
TseFI GTSAC 2 cut(s) 140, 377
TseI GCWGC 1 cut(s) 323
Tsp45I GTSAC 2 cut(s) 140, 377
TspGWI ACGGA 1 cut(s) 159
Tth111I GACNNNGTC 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 70
XmiI GTMKAC 2 cut(s) 29, 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.