MD03G1292500.v1.1

Belongs to the BetVI family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009655
Physical Location & Seq
Reverse (-)
36943314 .. 36945509
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1292500.v1.1.491

Sequence Viewer

Length: 483 bp
ATGAGTTCCATAAGTTTGAGCCAGGAATTTACAAGTCCAGTTGCAGCAGACAGGTTGTTCAAGGCCTTGATCATAGACTCCCACAATCTCATCCCCAAGCTCATGCCTCAGGCCATCAAGAGCATCGAAATCATCCACGGCGATGGCGGCGCAGGAAGCATCAAGCAGATCAACTTTGCTGAGGGTACGCGTTATAAGTACATGAAGAACAGAATAGACGCATTGGACGTGGAGAATTTGGTGTGCAAGTACACTCTGATTGAAGGAGATGTGCTGGCGGACAAGATTAAGTCCATTTCTTATGAGGTAAGCTTTCAAGCTTCCCCTAACGGCGGATGTACTTGTAAGATGATGAGTGAGTACCACGCCGCCGGTGACTTGGAGATCAATGAGGAAGAAATCAGGGGTGGCAAAGAAAGAGCAATGGGCATGTACAAAGTCGTTGAAGCTTACCTCTTCGAAAGTCCCGGTGCTTACGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

161

Amino Acids

17.67

Weight (kDa)

5.15

Isoelectric Point (pI)

50.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 3 - 153 4.1e-26 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 195
AccII CGCG 1 cut(s) 190
AciI CCGC 4 cut(s) 147, 278, 333, 369
AcsI RAATTY 2 cut(s) 26, 235
AfaI GTAC 6 cut(s) 187, 200, 251, 340, 362, 434
AfiI CCNNNNNNNGG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 188
AgsI TTSAA 4 cut(s) 61, 263, 317, 446
AjiI CACGTC 1 cut(s) 229
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 4 cut(s) 100, 312, 320, 449
AluI AGCT 4 cut(s) 100, 312, 320, 449
AoxI GGCC 2 cut(s) 63, 111
ApeKI GCWGC 1 cut(s) 44
ApoI RAATTY 2 cut(s) 26, 235
AspLEI GCGC 1 cut(s) 152
AsuC2I CCSGG 1 cut(s) 468
AsuHPI GGTGA 1 cut(s) 386
AsuII TTCGAA 1 cut(s) 459
AxyI CCTNAGG 1 cut(s) 108
BbvCI CCTCAGC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 56
BccI CCATC 2 cut(s) 122, 137
BceAI ACGGC 2 cut(s) 154, 346
BciT130I CCWGG 1 cut(s) 23
BclI TGATCA 1 cut(s) 69
BcnI CCSGG 1 cut(s) 468
BisI GCNGC 3 cut(s) 45, 148, 369
BlsI GCNGC 3 cut(s) 46, 149, 370
Bme1390I CCNGG 2 cut(s) 23, 468
BmgBI CACGTC 1 cut(s) 229
BmrFI CCNGG 2 cut(s) 23, 468
BmsI GCATC 2 cut(s) 132, 168
Bpu10I CCTNAGC 1 cut(s) 180
Bpu14I TTCGAA 1 cut(s) 459
BpuMI CCSGG 1 cut(s) 468
BsaJI CCNNGG 1 cut(s) 136
Bsc4I CCNNNNNNNGG 1 cut(s) 332
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 1 cut(s) 38
Bse21I CCTNAGG 1 cut(s) 108
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 3 cut(s) 90, 132, 341
BseLI CCNNNNNNNGG 1 cut(s) 332
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 2 cut(s) 122, 171
BseNI ACTGG 1 cut(s) 38
BseXI GCAGC 1 cut(s) 56
Bsh1236I CGCG 1 cut(s) 190
BshFI GGCC 2 cut(s) 65, 113
BsiSI CCGG 2 cut(s) 372, 468
BslFI GGGAC 1 cut(s) 450
BslI CCNNNNNNNGG 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 2 cut(s) 65, 113
Bsp119I TTCGAA 1 cut(s) 459
Bsp1407I TGTACA 1 cut(s) 432
Bsp143I GATC 3 cut(s) 69, 168, 384
BspACI CCGC 4 cut(s) 147, 278, 333, 369
BspANI GGCC 2 cut(s) 65, 113
BspCNI CTCAG 2 cut(s) 121, 172
BspFNI CGCG 1 cut(s) 190
BspT104I TTCGAA 1 cut(s) 459
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 1 cut(s) 371
BsrGI TGTACA 1 cut(s) 432
BsrI ACTGG 1 cut(s) 38
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 3 cut(s) 69, 168, 384
Bst2UI CCWGG 1 cut(s) 23
Bst6I CTCTTC 1 cut(s) 461
BstAUI TGTACA 1 cut(s) 432
BstBI TTCGAA 1 cut(s) 459
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 108, 180
BstDSI CCRYGG 1 cut(s) 136
BstF5I GGATG 3 cut(s) 90, 132, 341
BstFNI CGCG 1 cut(s) 190
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 3 cut(s) 72, 171, 387
BstMBI GATC 3 cut(s) 69, 168, 384
BstMWI GCNNNNNNNGC 2 cut(s) 147, 156
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 1 cut(s) 433
BstSCI CCNGG 2 cut(s) 21, 466
BstUI CGCG 1 cut(s) 190
BstV1I GCAGC 1 cut(s) 56
BstXI CCANNNNNNTGG 1 cut(s) 143
Bsu36I CCTNAGG 1 cut(s) 108
BsuRI GGCC 2 cut(s) 65, 113
BtgI CCRYGG 1 cut(s) 136
BtgZI GCGATG 1 cut(s) 156
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 3 cut(s) 90, 132, 341
Cac8I GCNNGC 1 cut(s) 276
CfoI GCGC 1 cut(s) 152
Cfr10I RCCGGY 1 cut(s) 371
CseI GACGC 1 cut(s) 227
Csp6I GTAC 6 cut(s) 186, 199, 250, 339, 361, 433
CviAII CATG 3 cut(s) 103, 202, 430
CviJI RGCY 7 cut(s) 21, 65, 100, 113, 312, 320, 449
CviKI_1 RGCY 7 cut(s) 21, 65, 100, 113, 312, 320, 449
CviQI GTAC 6 cut(s) 186, 199, 250, 339, 361, 433
DdeI CTNAG 2 cut(s) 108, 180
DpnI GATC 3 cut(s) 71, 170, 386
DpnII GATC 3 cut(s) 69, 168, 384
Eam1104I CTCTTC 1 cut(s) 461
EarI CTCTTC 1 cut(s) 461
EciI GGCGGA 2 cut(s) 293, 348
Eco147I AGGCCT 1 cut(s) 65
Eco81I CCTNAGG 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 21
FaeI CATG 3 cut(s) 106, 205, 433
FaiI YATR 7 cut(s) 11, 74, 104, 195, 203, 303, 431
FaqI GGGAC 1 cut(s) 450
FatI CATG 3 cut(s) 102, 201, 429
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 45, 148, 369
FokI GGATG 3 cut(s) 77, 119, 348
Fsp4HI GCNGC 3 cut(s) 45, 148, 369
GlaI GCGC 1 cut(s) 151
GluI GCNGC 3 cut(s) 45, 148, 369
HaeIII GGCC 2 cut(s) 65, 113
HapII CCGG 2 cut(s) 372, 468
HgaI GACGC 1 cut(s) 227
HhaI GCGC 1 cut(s) 152
Hin1II CATG 3 cut(s) 106, 205, 433
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HindIII AAGCTT 3 cut(s) 310, 318, 447
HinfI GANTC 1 cut(s) 77
HpaII CCGG 2 cut(s) 372, 468
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 1 cut(s) 252
Hpy188I TCNGA 1 cut(s) 258
Hpy188III TCNNGA 1 cut(s) 118
Hpy8I GTNNAC 1 cut(s) 252
HpyAV CCTTC 1 cut(s) 257
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 2 cut(s) 44, 246
HpyF10VI GCNNNNNNNGC 2 cut(s) 147, 156
HpyF3I CTNAG 2 cut(s) 108, 180
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 3 cut(s) 106, 205, 433
HspAI GCGC 1 cut(s) 150
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 3 cut(s) 69, 168, 384
LpnPI CCDG 9 cut(s) 8, 35, 37, 51, 95, 138, 260, 385, 388
Lsp1109I GCAGC 1 cut(s) 56
LweI GCATC 2 cut(s) 132, 168
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 1 cut(s) 374
MalI GATC 3 cut(s) 71, 170, 386
MboI GATC 3 cut(s) 69, 168, 384
MboII GAAGA 3 cut(s) 217, 407, 448
MluCI AATT 2 cut(s) 26, 235
MluI ACGCGT 1 cut(s) 188
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 5 cut(s) 117, 175, 298, 385, 464
MseI TTAA 1 cut(s) 288
MslI CAYNNNNRTG 1 cut(s) 141
MspI CCGG 2 cut(s) 372, 468
MspR9I CCNGG 2 cut(s) 23, 468
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 2 cut(s) 147, 156
NciI CCSGG 1 cut(s) 468
NdeII GATC 3 cut(s) 69, 168, 384
NlaIII CATG 3 cut(s) 106, 205, 433
NmuCI GTSAC 1 cut(s) 374
NspI RCATGY 1 cut(s) 433
NspV TTCGAA 1 cut(s) 459
PceI AGGCCT 1 cut(s) 65
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PkrI GCNGC 3 cut(s) 46, 149, 370
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PsiI TTATAA 1 cut(s) 195
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
RsaI GTAC 6 cut(s) 187, 200, 251, 340, 362, 434
RsaNI GTAC 6 cut(s) 186, 199, 250, 339, 361, 433
RseI CAYNNNNRTG 1 cut(s) 141
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 3 cut(s) 45, 148, 369
Sau3AI GATC 3 cut(s) 69, 168, 384
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 2 cut(s) 23, 468
SetI ASST 8 cut(s) 56, 102, 231, 309, 314, 322, 451, 456
SfaNI GCATC 2 cut(s) 132, 168
SfuI TTCGAA 1 cut(s) 459
SgrAI CRCCGGYG 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 141
Sse9I AATT 2 cut(s) 26, 235
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 4 cut(s) 147, 278, 333, 369
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 2 cut(s) 21, 466
TaiI ACGT 1 cut(s) 231
TaqI TCGA 2 cut(s) 126, 459
TasI AATT 2 cut(s) 26, 235
TatI WGTACW 4 cut(s) 198, 249, 338, 432
TauI GCSGC 2 cut(s) 150, 371
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 1 cut(s) 44
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 1 cut(s) 218
XapI RAATTY 2 cut(s) 26, 235
XceI RCATGY 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.