FvH4_4g18970

Major allergen Pru ar 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009655
Physical Location & Seq
Reverse (-)
22719273 .. 22721799
2527 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18970.t1

Sequence Viewer

Length: 639 bp
ATGGGTTGGATTATGCACATAAGGCCGAGATCTATTGGTTCTCACCAGTTGCCACATTCATGGCTAGTGCGGTTGGGGGACGGAATCTGGGAGTCTCACACAGACTTGGGTCTCGCCGTCTCGTCTCTTTCTCTGTCGAAGAAGAAGAAGAAAGGGAAGAAGAAGGAAGGTGAAGAAGATCGGTTTCTCACTATGGGGGTGACCACCTATACAGATGAGTACACTTCCCCAATCCCTCCATGCAGGTTGTTCAAGGCCTTAGTCCTTGATGCTGACATCCTTGTCCCAAAGCTCATGCCTGATGCCCTTAAGAGCATCGACACCATCCAAGGTGGGCAGATCATTTCTGTGCGAAATAGGGTTGATGAGGTGGATGATGAGAACTATGTGTATAATTACACCTTGATTGAAGGAGATGCTTCGGTAATGGAGAAAGTTGAGTTTATTTCTTATGAGGTTAAGTTTGAGGCCACACCAGAAGGTGGCAGTAAGAACAGGATGGTGAGCAAATACCACACCAAAGGTGACATTGTGCTCAATGACGAAGACATGATTTCTGGCAGAGAAAAGGCCTTGGGGATGTACAAAGTTGTGGAAGCCTACCTCCTCCAAAACCCAGATGTCTATAATGATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

213

Amino Acids

23.96

Weight (kDa)

5.44

Isoelectric Point (pI)

26.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 68 - 205 8.7e-08 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 281
Acc36I ACCTGC 1 cut(s) 234
AccB7I CCANNNNNTGG 1 cut(s) 482
AciI CCGC 1 cut(s) 70
AfaI GTAC 2 cut(s) 221, 584
AfiI CCNNNNNNNGG 1 cut(s) 482
AflII CTTAAG 1 cut(s) 308
AgsI TTSAA 2 cut(s) 253, 410
AluBI AGCT 1 cut(s) 292
AluI AGCT 1 cut(s) 292
Alw21I GWGCWC 1 cut(s) 537
Alw26I GTCTC 4 cut(s) 99, 116, 124, 129
AoxI GGCC 4 cut(s) 23, 255, 468, 570
AsuHPI GGTGA 5 cut(s) 35, 182, 211, 514, 536
BbsI GAAGAC 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 537
BccI CCATC 2 cut(s) 332, 493
BceAI ACGGC 1 cut(s) 101
BcoDI GTCTC 4 cut(s) 99, 116, 124, 129
BfaI CTAG 1 cut(s) 65
BfrI CTTAAG 1 cut(s) 308
BfuAI ACCTGC 1 cut(s) 234
BglII AGATCT 1 cut(s) 29
BmsI GCATC 4 cut(s) 259, 292, 324, 406
BoxI GACNNNNGTC 1 cut(s) 108
BpiI GAAGAC 1 cut(s) 552
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 328, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse1I ACTGG 1 cut(s) 46
BseDI CCNNGG 2 cut(s) 328, 573
BseGI GGATG 5 cut(s) 276, 324, 379, 504, 585
BseLI CCNNNNNNNGG 1 cut(s) 482
BseNI ACTGG 1 cut(s) 46
BseRI GAGGAG 1 cut(s) 596
BshFI GGCC 4 cut(s) 25, 257, 470, 572
BsiHKAI GWGCWC 1 cut(s) 537
BslFI GGGAC 2 cut(s) 92, 269
BslI CCNNNNNNNGG 1 cut(s) 482
BsmAI GTCTC 4 cut(s) 99, 116, 124, 129
BsmBI CGTCTC 2 cut(s) 124, 129
BsmFI GGGAC 2 cut(s) 92, 269
BsnI GGCC 4 cut(s) 25, 257, 470, 572
Bso31I GGTCTC 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 537
Bsp1407I TGTACA 1 cut(s) 582
Bsp143I GATC 3 cut(s) 29, 178, 339
BspACI CCGC 1 cut(s) 70
BspANI GGCC 4 cut(s) 25, 257, 470, 572
BspMI ACCTGC 1 cut(s) 234
BspTI CTTAAG 1 cut(s) 308
BspTNI GGTCTC 1 cut(s) 116
BsrGI TGTACA 1 cut(s) 582
BsrI ACTGG 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 328, 573
BssMI GATC 3 cut(s) 29, 178, 339
BssT1I CCWWGG 2 cut(s) 328, 573
BstAFI CTTAAG 1 cut(s) 308
BstAUI TGTACA 1 cut(s) 582
BstDEI CTNAG 1 cut(s) 259
BstEII GGTNACC 1 cut(s) 199
BstF5I GGATG 5 cut(s) 276, 324, 379, 504, 585
BstKTI GATC 3 cut(s) 32, 181, 342
BstMAI GTCTC 4 cut(s) 99, 116, 124, 129
BstMBI GATC 3 cut(s) 29, 178, 339
BstMWI GCNNNNNNNGC 1 cut(s) 22
BstPAI GACNNNNGTC 1 cut(s) 108
BstPI GGTNACC 1 cut(s) 199
BstV2I GAAGAC 1 cut(s) 552
BstX2I RGATCY 1 cut(s) 29
BstXI CCANNNNNNTGG 1 cut(s) 60
BstYI RGATCY 1 cut(s) 29
BsuRI GGCC 4 cut(s) 25, 257, 470, 572
BtsCI GGATG 5 cut(s) 276, 324, 379, 504, 585
BveI ACCTGC 1 cut(s) 234
Csp6I GTAC 2 cut(s) 220, 583
CviAII CATG 4 cut(s) 60, 240, 295, 550
CviJI RGCY 7 cut(s) 25, 64, 257, 292, 470, 572, 599
CviKI_1 RGCY 7 cut(s) 25, 64, 257, 292, 470, 572, 599
CviQI GTAC 2 cut(s) 220, 583
DdeI CTNAG 1 cut(s) 259
DpnI GATC 3 cut(s) 31, 180, 341
DpnII GATC 3 cut(s) 29, 178, 339
DrdI GACNNNNNNGTC 1 cut(s) 281
DseDI GACNNNNNNGTC 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 328, 573
Eco147I AGGCCT 2 cut(s) 257, 572
Eco31I GGTCTC 1 cut(s) 116
Eco91I GGTNACC 1 cut(s) 199
EcoO65I GGTNACC 1 cut(s) 199
EcoT14I CCWWGG 2 cut(s) 328, 573
ErhI CCWWGG 2 cut(s) 328, 573
Esp3I CGTCTC 2 cut(s) 124, 129
FaeI CATG 4 cut(s) 63, 243, 298, 553
FaqI GGGAC 2 cut(s) 92, 269
FatI CATG 4 cut(s) 59, 239, 294, 549
FokI GGATG 5 cut(s) 263, 311, 386, 511, 592
FspBI CTAG 1 cut(s) 65
HaeIII GGCC 4 cut(s) 25, 257, 470, 572
Hin1II CATG 4 cut(s) 63, 243, 298, 553
HinfI GANTC 2 cut(s) 84, 92
HphI GGTGA 5 cut(s) 35, 182, 211, 514, 536
Hpy166II GTNNAC 1 cut(s) 222
Hpy8I GTNNAC 1 cut(s) 222
HpyAV CCTTC 4 cut(s) 157, 161, 404, 473
HpyCH4V TGCA 2 cut(s) 16, 243
HpyF10VI GCNNNNNNNGC 1 cut(s) 22
HpyF3I CTNAG 1 cut(s) 259
Hsp92II CATG 4 cut(s) 63, 243, 298, 553
Kzo9I GATC 3 cut(s) 29, 178, 339
LpnPI CCDG 8 cut(s) 59, 73, 229, 312, 481, 489, 543, 630
LweI GCATC 4 cut(s) 259, 292, 324, 406
MaeI CTAG 1 cut(s) 65
MaeIII GTNAC 2 cut(s) 199, 524
MalI GATC 3 cut(s) 31, 180, 341
MboI GATC 3 cut(s) 29, 178, 339
MboII GAAGA 9 cut(s) 151, 154, 157, 160, 169, 172, 185, 188, 557
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 1 cut(s) 537
MluCI AATT 1 cut(s) 394
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 6 cut(s) 246, 361, 448, 460, 614, 617
MseI TTAA 2 cut(s) 309, 459
MslI CAYNNNNRTG 2 cut(s) 58, 347
MspCI CTTAAG 1 cut(s) 308
MwoI GCNNNNNNNGC 1 cut(s) 22
NdeII GATC 3 cut(s) 29, 178, 339
NlaIII CATG 4 cut(s) 63, 243, 298, 553
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 2 cut(s) 199, 524
PceI AGGCCT 2 cut(s) 257, 572
PfeI GAWTC 1 cut(s) 84
PflMI CCANNNNNTGG 1 cut(s) 482
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
PshAI GACNNNNGTC 1 cut(s) 108
PspEI GGTNACC 1 cut(s) 199
PsuI RGATCY 1 cut(s) 29
RsaI GTAC 2 cut(s) 221, 584
RsaNI GTAC 2 cut(s) 220, 583
RseI CAYNNNNRTG 2 cut(s) 58, 347
SaqAI TTAA 2 cut(s) 309, 459
Sau3AI GATC 3 cut(s) 29, 178, 339
SchI GAGTC 1 cut(s) 101
SduI GDGCHC 1 cut(s) 537
SfaNI GCATC 4 cut(s) 259, 292, 324, 406
SmiMI CAYNNNNRTG 2 cut(s) 58, 347
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 1 cut(s) 394
SseBI AGGCCT 2 cut(s) 257, 572
SsiI CCGC 1 cut(s) 70
SspMI CTAG 1 cut(s) 65
StuI AGGCCT 2 cut(s) 257, 572
StyI CCWWGG 2 cut(s) 328, 573
TaqI TCGA 2 cut(s) 137, 318
TasI AATT 1 cut(s) 394
TatI WGTACW 2 cut(s) 219, 582
TfiI GAWTC 1 cut(s) 84
Tru1I TTAA 2 cut(s) 309, 459
Tru9I TTAA 2 cut(s) 309, 459
TseFI GTSAC 2 cut(s) 199, 524
Tsp45I GTSAC 2 cut(s) 199, 524
TspDTI ATGAA 1 cut(s) 48
TspGWI ACGGA 1 cut(s) 96
Van91I CCANNNNNTGG 1 cut(s) 482
Vha464I CTTAAG 1 cut(s) 308
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.