FvH4_2g27850

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009190
Physical Location & Seq
Forward (+)
22001760 .. 22005068
3309 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27850.t2

Sequence Viewer

Length: 993 bp
ATGAGAGCCACCGCTCTCTTCCTCTCTCCGCCGCCGCTCTTCACCTCCCACAAGCCTCTCTCCTCACCCACCACCGCCCCTCTCCTCCTCCACTCTTCCCTCCCCCTCCCTCTCCGCTCCCTCAAACCCAACCCCACCCTCAGCCGCCCGTCCTTCCTCGTCCGCGCCGACGACGCCGACGCCGACGGAGGCGGCCCCGACGACTACGACATGGACGACGACGAGCTCGAGGAGGTCGACAACAAGAAGGACTTCGACATCGAATACGACCCCCTCGCCGCCGCGGCTGCCGCCGTCGCCGCCGCCAACGGCGATGTCGACATCGCCATGGTGCAGAGCAAGAGCTTCGTCCATACGCAGGGATGGGACTCCGAGATGGTGGTGGATTATCGGATCAACGAGGACGAGTTTCACAAGATAAGCTTGCTCGATTGTGATTTCTTTATCAGGAAACCGCCGGACCCCGATAACGACGTCTTCGATTTCCGAGAGATGTATGTTACTCCTCCGGATACAGATGTTTATGCCATTCCTAAGGTTTTAGCTCCAATGCCTGACAAGTACATTCGATGTGCCAAGAGTGAATATGCATCCTACAATGTGACTGAACCGCCGATTGATGCTCCTAGAGATCCATTGTACAAGACCGAGAGGGACATTCTGAAGGTTTATTTGACAAAGCACTACAGAAACCGGCGCTTGGGAGACCCTGATTTTGTACTGGATTTTGAAGAGATTTATGTAATTGATTCCAAGACAAAGTCTATTAGCAGAGCGAAAGTTCTGGTCACTGTTCCAGGGGGAAAAACAAGAGATAGAAGACATGATCTGCTTGTAATACGTGACAATGGCAACACCTTCAGAATAGTTCATGGGAGTGAAAAAGATGATCCCACCACAGTGATAGAGAGGGAAGAGTGGAATAAGACCAGAAAGGACATGGAGAGACATCTTAGCAAGCTACGAGATTTCCCGGTTTCAAATTGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

331

Amino Acids

37.66

Weight (kDa)

4.97

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 477
AccBSI CCGCTC 3 cut(s) 14, 37, 117
AccI GTMKAC 2 cut(s) 237, 318
AccII CGCG 2 cut(s) 165, 284
AccIII TCCGGA 1 cut(s) 508
AclWI GGATC 3 cut(s) 401, 626, 884
AcuI CTGAAG 2 cut(s) 683, 844
AcyI GRCGYC 3 cut(s) 174, 180, 474
AfaI GTAC 3 cut(s) 563, 641, 720
AfiI CCNNNNNNNGG 2 cut(s) 358, 700
AgsI TTSAA 2 cut(s) 731, 981
AjnI CCWGG 1 cut(s) 796
AleI CACNNNNGTG 1 cut(s) 899
AluBI AGCT 5 cut(s) 226, 345, 423, 545, 961
AluI AGCT 5 cut(s) 226, 345, 423, 545, 961
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 2 cut(s) 699, 940
AlwI GGATC 3 cut(s) 401, 626, 884
Ama87I CYCGRG 1 cut(s) 227
Aor13HI TCCGGA 1 cut(s) 508
AoxI GGCC 1 cut(s) 193
ApeKI GCWGC 1 cut(s) 287
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
Asp700I GAANNNNTTC 1 cut(s) 251
AspLEI GCGC 2 cut(s) 167, 699
AspS9I GGNCC 2 cut(s) 194, 460
AsuC2I CCSGG 1 cut(s) 974
AsuHPI GGTGA 2 cut(s) 34, 57
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 1 cut(s) 460
AxyI CCTNAGG 1 cut(s) 534
BaeI ACNNNNGTAYC 2 cut(s) 504, 537
BanII GRGCYC 1 cut(s) 228
BbsI GAAGAC 2 cut(s) 469, 826
Bbv12I GWGCWC 1 cut(s) 228
BbvCI CCTCAGC 1 cut(s) 140
BbvI GCAGC 1 cut(s) 274
BccI CCATC 2 cut(s) 357, 370
BceAI ACGGC 2 cut(s) 278, 325
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 1 cut(s) 798
BciVI GTATCC 1 cut(s) 505
BcnI CCSGG 1 cut(s) 974
BcoDI GTCTC 2 cut(s) 699, 940
BfaI CTAG 2 cut(s) 627, 991
BfmI CTRYAG 1 cut(s) 685
BfoI RGCGCY 1 cut(s) 700
BfuI GTATCC 1 cut(s) 505
BglI GCCNNNNNGGC 1 cut(s) 284
Bme1390I CCNGG 2 cut(s) 798, 974
Bme18I GGWCC 1 cut(s) 460
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 2 cut(s) 194, 460
BmiI GGNNCC 2 cut(s) 196, 462
BmrFI CCNGG 2 cut(s) 798, 974
BmsI GCATC 2 cut(s) 599, 610
BpiI GAAGAC 2 cut(s) 469, 826
Bpu10I CCTNAGC 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 974
BsaAI YACGTR 1 cut(s) 842
BsaHI GRCGYC 3 cut(s) 174, 180, 474
BsaI GGTCTC 1 cut(s) 699
BsaJI CCNNGG 3 cut(s) 282, 327, 797
BsaWI WCCGGW 1 cut(s) 508
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 700
Bse118I RCCGGY 1 cut(s) 693
Bse1I ACTGG 1 cut(s) 726
Bse21I CCTNAGG 1 cut(s) 534
BseAI TCCGGA 1 cut(s) 508
BseBI CCWGG 1 cut(s) 798
BseDI CCNNGG 3 cut(s) 282, 327, 797
BseGI GGATG 2 cut(s) 368, 590
BseLI CCNNNNNNNGG 2 cut(s) 358, 700
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 1 cut(s) 726
BseRI GAGGAG 5 cut(s) 52, 74, 77, 245, 495
BseXI GCAGC 1 cut(s) 274
BsgI GTGCAG 1 cut(s) 353
Bsh1236I CGCG 2 cut(s) 165, 284
BshFI GGCC 1 cut(s) 195
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 4 cut(s) 458, 509, 694, 974
BslFI GGGAC 2 cut(s) 380, 668
BslI CCNNNNNNNGG 2 cut(s) 358, 700
BsmAI GTCTC 2 cut(s) 699, 940
BsmFI GGGAC 2 cut(s) 380, 668
BsnI GGCC 1 cut(s) 195
Bso31I GGTCTC 1 cut(s) 699
BsoBI CYCGRG 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 228
Bsp13I TCCGGA 1 cut(s) 508
Bsp1407I TGTACA 1 cut(s) 639
Bsp143I GATC 4 cut(s) 393, 631, 826, 889
Bsp19I CCATGG 1 cut(s) 327
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 1 cut(s) 153
BspEI TCCGGA 1 cut(s) 508
BspFNI CGCG 2 cut(s) 165, 284
BspLI GGNNCC 2 cut(s) 196, 462
BspPI GGATC 3 cut(s) 401, 626, 884
BspQI GCTCTTC 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 699
BsrBI CCGCTC 3 cut(s) 14, 37, 117
BsrFI RCCGGY 1 cut(s) 693
BsrGI TGTACA 1 cut(s) 639
BsrI ACTGG 1 cut(s) 726
BssAI RCCGGY 1 cut(s) 693
BssECI CCNNGG 3 cut(s) 282, 327, 797
BssMI GATC 4 cut(s) 393, 631, 826, 889
BssNI GRCGYC 3 cut(s) 174, 180, 474
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 1 cut(s) 798
Bst4CI ACNGT 2 cut(s) 793, 901
Bst6I CTCTTC 5 cut(s) 23, 44, 100, 726, 909
BstACI GRCGYC 3 cut(s) 174, 180, 474
BstAUI TGTACA 1 cut(s) 639
BstBAI YACGTR 1 cut(s) 842
BstC8I GCNNGC 2 cut(s) 425, 959
BstDEI CTNAG 3 cut(s) 140, 534, 953
BstDSI CCRYGG 2 cut(s) 282, 327
BstF5I GGATG 2 cut(s) 368, 590
BstFNI CGCG 2 cut(s) 165, 284
BstH2I RGCGCY 1 cut(s) 700
BstHHI GCGC 2 cut(s) 167, 699
BstKTI GATC 4 cut(s) 396, 634, 829, 892
BstMAI GTCTC 2 cut(s) 699, 940
BstMBI GATC 4 cut(s) 393, 631, 826, 889
BstMWI GCNNNNNNNGC 6 cut(s) 173, 284, 287, 290, 296, 299
BstNI CCWGG 1 cut(s) 798
BstSCI CCNGG 2 cut(s) 796, 972
BstSFI CTRYAG 1 cut(s) 685
BstUI CGCG 2 cut(s) 165, 284
BstV1I GCAGC 1 cut(s) 274
BstV2I GAAGAC 2 cut(s) 469, 826
BstX2I RGATCY 1 cut(s) 631
BstYI RGATCY 1 cut(s) 631
Bsu36I CCTNAGG 1 cut(s) 534
BsuI GTATCC 1 cut(s) 505
BsuRI GGCC 1 cut(s) 195
BtgI CCRYGG 2 cut(s) 282, 327
BtgZI GCGATG 2 cut(s) 307, 327
BtsCI GGATG 2 cut(s) 368, 590
BtsIMutI CAGTG 2 cut(s) 789, 906
Cac8I GCNNGC 2 cut(s) 425, 959
CfoI GCGC 2 cut(s) 167, 699
Cfr10I RCCGGY 1 cut(s) 693
Cfr13I GGNCC 2 cut(s) 194, 460
Cfr42I CCGCGG 1 cut(s) 285
CseI GACGC 2 cut(s) 182, 188
Csp6I GTAC 3 cut(s) 562, 640, 719
CviAII CATG 5 cut(s) 211, 328, 824, 872, 940
CviQI GTAC 3 cut(s) 562, 640, 719
DdeI CTNAG 3 cut(s) 140, 534, 953
DpnI GATC 4 cut(s) 395, 633, 828, 891
DpnII GATC 4 cut(s) 393, 631, 826, 889
Eam1104I CTCTTC 5 cut(s) 23, 44, 100, 726, 909
EarI CTCTTC 5 cut(s) 23, 44, 100, 726, 909
EciI GGCGGA 1 cut(s) 18
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 699
Eco47I GGWCC 1 cut(s) 460
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 2 cut(s) 683, 844
Eco81I CCTNAGG 1 cut(s) 534
Eco88I CYCGRG 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 796
EcoT14I CCWWGG 1 cut(s) 327
EcoT22I ATGCAT 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 5 cut(s) 214, 331, 827, 875, 943
FalI AAGNNNNNCTT 4 cut(s) 236, 268, 407, 439
FaqI GGGAC 2 cut(s) 380, 668
FatI CATG 5 cut(s) 210, 327, 823, 871, 939
FblI GTMKAC 2 cut(s) 237, 318
FokI GGATG 2 cut(s) 375, 577
FriOI GRGCYC 1 cut(s) 228
FspBI CTAG 2 cut(s) 627, 991
GlaI GCGC 2 cut(s) 166, 698
HaeII RGCGCY 1 cut(s) 700
HaeIII GGCC 1 cut(s) 195
HapII CCGG 4 cut(s) 458, 509, 694, 974
HgaI GACGC 2 cut(s) 182, 188
HhaI GCGC 2 cut(s) 167, 699
Hin1I GRCGYC 3 cut(s) 174, 180, 474
Hin1II CATG 5 cut(s) 214, 331, 827, 875, 943
Hin6I GCGC 2 cut(s) 165, 697
HinP1I GCGC 2 cut(s) 165, 697
HincII GTYRAC 2 cut(s) 238, 319
HindII GTYRAC 2 cut(s) 238, 319
HindIII AAGCTT 1 cut(s) 421
HinfI GANTC 2 cut(s) 368, 749
HpaII CCGG 4 cut(s) 458, 509, 694, 974
HphI GGTGA 2 cut(s) 34, 57
Hpy166II GTNNAC 2 cut(s) 238, 319
Hpy188I TCNGA 5 cut(s) 373, 393, 488, 663, 863
Hpy188III TCNNGA 2 cut(s) 448, 509
Hpy8I GTNNAC 2 cut(s) 238, 319
Hpy99I CGWCG 9 cut(s) 173, 176, 182, 188, 203, 221, 224, 299, 476
HpyAV CCTTC 4 cut(s) 163, 241, 658, 868
HpyCH4III ACNGT 2 cut(s) 793, 901
HpyCH4IV ACGT 2 cut(s) 474, 841
HpyCH4V TGCA 2 cut(s) 334, 590
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 284, 287, 290, 296, 299
HpyF3I CTNAG 3 cut(s) 140, 534, 953
HpySE526I ACGT 2 cut(s) 474, 841
Hsp92I GRCGYC 3 cut(s) 174, 180, 474
Hsp92II CATG 5 cut(s) 214, 331, 827, 875, 943
HspAI GCGC 2 cut(s) 165, 697
Kpn2I TCCGGA 1 cut(s) 508
KspI CCGCGG 1 cut(s) 285
Kzo9I GATC 4 cut(s) 393, 631, 826, 889
LguI GCTCTTC 1 cut(s) 44
LmnI GCTCC 3 cut(s) 122, 550, 628
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 2 cut(s) 599, 610
MaeI CTAG 2 cut(s) 627, 991
MaeII ACGT 2 cut(s) 474, 841
MaeIII GTNAC 4 cut(s) 499, 601, 787, 842
MalI GATC 4 cut(s) 395, 633, 828, 891
MbiI CCGCTC 3 cut(s) 14, 37, 117
MboI GATC 4 cut(s) 393, 631, 826, 889
MboII GAAGA 7 cut(s) 10, 31, 87, 469, 743, 831, 926
MflI RGATCY 1 cut(s) 631
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 2 cut(s) 744, 982
MlyI GAGTC 1 cut(s) 362
Mph1103I ATGCAT 1 cut(s) 592
MroI TCCGGA 1 cut(s) 508
MroXI GAANNNNTTC 1 cut(s) 251
MslI CAYNNNNRTG 3 cut(s) 326, 876, 899
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 4 cut(s) 458, 509, 694, 974
MspR9I CCNGG 2 cut(s) 798, 974
MvaI CCWGG 1 cut(s) 798
MvnI CGCG 2 cut(s) 165, 284
MwoI GCNNNNNNNGC 6 cut(s) 173, 284, 287, 290, 296, 299
NciI CCSGG 1 cut(s) 974
NcoI CCATGG 1 cut(s) 327
NdeII GATC 4 cut(s) 393, 631, 826, 889
NlaIII CATG 5 cut(s) 214, 331, 827, 875, 943
NlaIV GGNNCC 2 cut(s) 196, 462
NmuCI GTSAC 3 cut(s) 601, 787, 842
NsiI ATGCAT 1 cut(s) 592
OliI CACNNNNGTG 1 cut(s) 899
PaeR7I CTCGAG 1 cut(s) 227
PciSI GCTCTTC 1 cut(s) 44
PcsI WCGNNNNNNNCGW 7 cut(s) 165, 180, 213, 225, 234, 315, 477
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 1 cut(s) 749
PflFI GACNNNGTC 1 cut(s) 760
PleI GAGTC 1 cut(s) 362
PpsI GAGTC 1 cut(s) 362
Ppu21I YACGTR 1 cut(s) 842
Psp124BI GAGCTC 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 796
PspGI CCWGG 1 cut(s) 796
PspN4I GGNNCC 2 cut(s) 196, 462
PspPI GGNCC 2 cut(s) 194, 460
PspXI VCTCGAGB 1 cut(s) 227
PsuI RGATCY 1 cut(s) 631
PsyI GACNNNGTC 1 cut(s) 760
RsaI GTAC 3 cut(s) 563, 641, 720
RsaNI GTAC 3 cut(s) 562, 640, 719
RseI CAYNNNNRTG 3 cut(s) 326, 876, 899
SacI GAGCTC 1 cut(s) 228
SacII CCGCGG 1 cut(s) 285
SalI GTCGAC 2 cut(s) 236, 317
SapI GCTCTTC 1 cut(s) 44
Sau3AI GATC 4 cut(s) 393, 631, 826, 889
Sau96I GGNCC 2 cut(s) 194, 460
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 2 cut(s) 798, 974
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 2 cut(s) 599, 610
SfcI CTRYAG 1 cut(s) 685
Sfr274I CTCGAG 1 cut(s) 227
Sfr303I CCGCGG 1 cut(s) 285
SgrBI CCGCGG 1 cut(s) 285
SinI GGWCC 1 cut(s) 460
SlaI CTCGAG 1 cut(s) 227
SmiMI CAYNNNNRTG 3 cut(s) 326, 876, 899
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 2 cut(s) 744, 982
SspMI CTAG 2 cut(s) 627, 991
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 2 cut(s) 796, 972
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 2 cut(s) 793, 901
TaiI ACGT 2 cut(s) 477, 844
TaqI TCGA 8 cut(s) 228, 237, 255, 261, 318, 429, 480, 568
TaqII GACCGA 1 cut(s) 662
TasI AATT 2 cut(s) 744, 982
TatI WGTACW 3 cut(s) 561, 639, 718
TfiI GAWTC 1 cut(s) 749
TscAI CASTG 2 cut(s) 796, 906
TseFI GTSAC 3 cut(s) 601, 787, 842
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 3 cut(s) 601, 787, 842
TspDTI ATGAA 1 cut(s) 860
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 2 cut(s) 796, 906
Tth111I GACNNNGTC 1 cut(s) 760
VpaK11BI GGWCC 1 cut(s) 460
XcmI CCANNNNNNNNNTGG 1 cut(s) 937
XhoI CTCGAG 1 cut(s) 227
XmiI GTMKAC 2 cut(s) 237, 318
XmnI GAANNNNTTC 1 cut(s) 251
XspI CTAG 2 cut(s) 627, 991
ZraI GACGTC 1 cut(s) 475
Zsp2I ATGCAT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.