Rroxscaffold_7G00171240

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009190
Physical Location & Seq
Reverse (-)
11570791 .. 11573278
2488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00171240.1

Sequence Viewer

Length: 717 bp
ATGAGAGCCACCGCTCTCTTCCTCTCTCCGCCGCCGCCGCCGCTCTTCACCCGCAAACCTCTCTTCACACCCACCACACCTCTCCTCCTCCACTCTTCCCACCCCCTCCCTTTCCGCTCCCTCAAACCCAAACCCGCCCTCAACCGCCCCTCCTTCCTCGTCCGCGCCGACGACGGCGACGGCGACGGAGGCGGCCCCGACGACTACGACATGGACGTTGAGGAGCTCGAGGAGATGTATGTTACTCCTCCGGATACAGATGTTTATGCCATTCCTAGGGTTTTAGCTCCAATGCCTGACAAGAGTGAATTTAGCTGCTACAATGTGACGGAACCGCCCATCGATGCTCCTAGAGACCCATTGTACAAGACCGAGAGGGACGTTTTTAAGGTTTATTTGACAAAGCACTACAGGAACCGGCGCGTGGGAGACCCTGATTTTGTACTGGATTTTGAAGAGATTTACGTGATTGATTCCAAGACAAAGTCTATTAGCAGAGCAAAAGTGCTGGTCAGTGTTCCGGGGGGAAGAACCAGAGATAGAAGACACGATCTGCTTGTAATACGTGACAATGGCAACTCCTTCAGAATAGTTCATGCGAGTGAAAAAGATGATCCCACCACAGTGATAGAGAGGGAAGAGTGGAATAAGACCAGAAAGGACATGGAGAGACATCTTAGCAAGCTACGAGATTTCCCCGTTTCAAATTGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

27.42

Weight (kDa)

6.1

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 14, 43, 117
AccII CGCG 2 cut(s) 165, 423
AccIII TCCGGA 1 cut(s) 250
AclWI GGATC 1 cut(s) 608
AcsI RAATTY 1 cut(s) 308
AcuI CTGAAG 1 cut(s) 568
AfaI GTAC 2 cut(s) 365, 444
AfiI CCNNNNNNNGG 2 cut(s) 276, 424
AgsI TTSAA 2 cut(s) 455, 705
AleI CACNNNNGTG 1 cut(s) 623
AluBI AGCT 4 cut(s) 226, 287, 315, 685
AluI AGCT 4 cut(s) 226, 287, 315, 685
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 3 cut(s) 348, 423, 664
AlwI GGATC 1 cut(s) 608
Ama87I CYCGRG 1 cut(s) 227
Aor13HI TCCGGA 1 cut(s) 250
AoxI GGCC 1 cut(s) 193
ApeKI GCWGC 1 cut(s) 315
ApoI RAATTY 1 cut(s) 308
ArsI GACNNNNNNTTYG 2 cut(s) 422, 454
AspA2I CCTAGG 1 cut(s) 275
AspLEI GCGC 2 cut(s) 167, 423
AspS9I GGNCC 1 cut(s) 194
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 1 cut(s) 40
AvaI CYCGRG 1 cut(s) 227
AvrII CCTAGG 1 cut(s) 275
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
BanII GRGCYC 1 cut(s) 228
BbsI GAAGAC 1 cut(s) 550
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 302
BccI CCATC 1 cut(s) 347
BceAI ACGGC 2 cut(s) 190, 196
BciVI GTATCC 1 cut(s) 247
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 3 cut(s) 348, 423, 664
BfaI CTAG 3 cut(s) 276, 351, 715
BfmI CTRYAG 1 cut(s) 409
BfuI GTATCC 1 cut(s) 247
BisI GCNGC 6 cut(s) 32, 35, 38, 41, 193, 316
BlnI CCTAGG 1 cut(s) 275
BlsI GCNGC 6 cut(s) 33, 36, 39, 42, 194, 317
Bme1390I CCNGG 1 cut(s) 522
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 3 cut(s) 196, 333, 416
BmrFI CCNGG 1 cut(s) 522
BmsI GCATC 1 cut(s) 334
BpiI GAAGAC 1 cut(s) 550
BpuMI CCSGG 1 cut(s) 522
Bsa29I ATCGAT 1 cut(s) 342
BsaAI YACGTR 2 cut(s) 466, 566
BsaI GGTCTC 2 cut(s) 348, 423
BsaJI CCNNGG 2 cut(s) 275, 521
BsaWI WCCGGW 1 cut(s) 250
BsaXI ACNNNNNCTCC 4 cut(s) 69, 99, 134, 164
Bsc4I CCNNNNNNNGG 2 cut(s) 276, 424
Bse118I RCCGGY 1 cut(s) 417
Bse1I ACTGG 1 cut(s) 450
BseAI TCCGGA 1 cut(s) 250
BseCI ATCGAT 1 cut(s) 342
BseDI CCNNGG 2 cut(s) 275, 521
BseLI CCNNNNNNNGG 2 cut(s) 276, 424
BseNI ACTGG 1 cut(s) 450
BseRI GAGGAG 5 cut(s) 74, 77, 236, 237, 245
BseXI GCAGC 1 cut(s) 302
Bsh1236I CGCG 2 cut(s) 165, 423
BshFI GGCC 1 cut(s) 195
BshVI ATCGAT 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 3 cut(s) 251, 418, 521
BslFI GGGAC 1 cut(s) 392
BslI CCNNNNNNNGG 2 cut(s) 276, 424
BsmAI GTCTC 3 cut(s) 348, 423, 664
BsmFI GGGAC 1 cut(s) 392
BsnI GGCC 1 cut(s) 195
Bso31I GGTCTC 2 cut(s) 348, 423
BsoBI CYCGRG 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 228
Bsp13I TCCGGA 1 cut(s) 250
Bsp1407I TGTACA 1 cut(s) 363
Bsp143I GATC 2 cut(s) 550, 613
BspANI GGCC 1 cut(s) 195
BspDI ATCGAT 1 cut(s) 342
BspEI TCCGGA 1 cut(s) 250
BspFNI CGCG 2 cut(s) 165, 423
BspLI GGNNCC 3 cut(s) 196, 333, 416
BspPI GGATC 1 cut(s) 608
BspQI GCTCTTC 1 cut(s) 50
BspTNI GGTCTC 2 cut(s) 348, 423
BsrBI CCGCTC 3 cut(s) 14, 43, 117
BsrFI RCCGGY 1 cut(s) 417
BsrGI TGTACA 1 cut(s) 363
BsrI ACTGG 1 cut(s) 450
BssAI RCCGGY 1 cut(s) 417
BssECI CCNNGG 2 cut(s) 275, 521
BssMI GATC 2 cut(s) 550, 613
BssT1I CCWWGG 1 cut(s) 275
Bst4CI ACNGT 1 cut(s) 625
Bst6I CTCTTC 6 cut(s) 23, 50, 68, 100, 450, 633
BstAUI TGTACA 1 cut(s) 363
BstBAI YACGTR 2 cut(s) 466, 566
BstC8I GCNNGC 1 cut(s) 683
BstDEI CTNAG 1 cut(s) 677
BstFNI CGCG 2 cut(s) 165, 423
BstHHI GCGC 2 cut(s) 167, 423
BstKTI GATC 2 cut(s) 553, 616
BstMAI GTCTC 3 cut(s) 348, 423, 664
BstMBI GATC 2 cut(s) 550, 613
BstMWI GCNNNNNNNGC 3 cut(s) 37, 40, 189
BstSCI CCNGG 1 cut(s) 520
BstSFI CTRYAG 1 cut(s) 409
BstUI CGCG 2 cut(s) 165, 423
BstV1I GCAGC 1 cut(s) 302
BstV2I GAAGAC 1 cut(s) 550
Bsu15I ATCGAT 1 cut(s) 342
BsuI GTATCC 1 cut(s) 247
BsuRI GGCC 1 cut(s) 195
BsuTUI ATCGAT 1 cut(s) 342
BtsIMutI CAGTG 2 cut(s) 520, 630
Cac8I GCNNGC 1 cut(s) 683
CfoI GCGC 2 cut(s) 167, 423
Cfr10I RCCGGY 1 cut(s) 417
Cfr13I GGNCC 1 cut(s) 194
ClaI ATCGAT 1 cut(s) 342
Csp6I GTAC 2 cut(s) 364, 443
CviAII CATG 3 cut(s) 211, 596, 664
CviJI RGCY 6 cut(s) 8, 195, 226, 287, 315, 685
CviKI_1 RGCY 6 cut(s) 8, 195, 226, 287, 315, 685
CviQI GTAC 2 cut(s) 364, 443
DdeI CTNAG 1 cut(s) 677
DpnI GATC 2 cut(s) 552, 615
DpnII GATC 2 cut(s) 550, 613
Eam1104I CTCTTC 6 cut(s) 23, 50, 68, 100, 450, 633
EarI CTCTTC 6 cut(s) 23, 50, 68, 100, 450, 633
EciI GGCGGA 1 cut(s) 18
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 228
Eco31I GGTCTC 2 cut(s) 348, 423
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 568
Eco88I CYCGRG 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 275
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 275
FaeI CATG 3 cut(s) 214, 599, 667
FaiI YATR 5 cut(s) 212, 240, 267, 597, 665
FaqI GGGAC 1 cut(s) 392
FatI CATG 3 cut(s) 210, 595, 663
FauI CCCGC 2 cut(s) 59, 142
Fnu4HI GCNGC 6 cut(s) 32, 35, 38, 41, 193, 316
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 6 cut(s) 32, 35, 38, 41, 193, 316
FspBI CTAG 3 cut(s) 276, 351, 715
GlaI GCGC 2 cut(s) 166, 422
GluI GCNGC 6 cut(s) 32, 35, 38, 41, 193, 316
HaeIII GGCC 1 cut(s) 195
HapII CCGG 3 cut(s) 251, 418, 521
HhaI GCGC 2 cut(s) 167, 423
Hin1II CATG 3 cut(s) 214, 599, 667
Hin6I GCGC 2 cut(s) 165, 421
HinP1I GCGC 2 cut(s) 165, 421
HinfI GANTC 1 cut(s) 473
HpaII CCGG 3 cut(s) 251, 418, 521
HphI GGTGA 1 cut(s) 40
Hpy188I TCNGA 1 cut(s) 587
Hpy188III TCNNGA 1 cut(s) 251
Hpy99I CGWCG 5 cut(s) 173, 176, 182, 188, 203
HpyAV CCTTC 2 cut(s) 163, 592
HpyCH4III ACNGT 1 cut(s) 625
HpyCH4IV ACGT 4 cut(s) 216, 381, 465, 565
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 40, 189
HpyF3I CTNAG 1 cut(s) 677
HpySE526I ACGT 4 cut(s) 216, 381, 465, 565
Hsp92II CATG 3 cut(s) 214, 599, 667
HspAI GCGC 2 cut(s) 165, 421
Kpn2I TCCGGA 1 cut(s) 250
Kzo9I GATC 2 cut(s) 550, 613
LguI GCTCTTC 1 cut(s) 50
LmnI GCTCC 4 cut(s) 122, 223, 292, 352
Lsp1109I GCAGC 1 cut(s) 302
LweI GCATC 1 cut(s) 334
MaeI CTAG 3 cut(s) 276, 351, 715
MaeII ACGT 4 cut(s) 216, 381, 465, 565
MaeIII GTNAC 3 cut(s) 241, 325, 566
MalI GATC 2 cut(s) 552, 615
MbiI CCGCTC 3 cut(s) 14, 43, 117
MboI GATC 2 cut(s) 550, 613
MboII GAAGA 8 cut(s) 10, 37, 55, 87, 467, 540, 555, 650
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 2 cut(s) 308, 706
MroI TCCGGA 1 cut(s) 250
MseI TTAA 1 cut(s) 387
MslI CAYNNNNRTG 2 cut(s) 600, 623
MspI CCGG 3 cut(s) 251, 418, 521
MspR9I CCNGG 1 cut(s) 522
MvnI CGCG 2 cut(s) 165, 423
MwoI GCNNNNNNNGC 3 cut(s) 37, 40, 189
NciI CCSGG 1 cut(s) 522
NdeII GATC 2 cut(s) 550, 613
NlaIII CATG 3 cut(s) 214, 599, 667
NlaIV GGNNCC 3 cut(s) 196, 333, 416
NmuCI GTSAC 2 cut(s) 325, 566
OliI CACNNNNGTG 1 cut(s) 623
PaeR7I CTCGAG 1 cut(s) 227
PciSI GCTCTTC 1 cut(s) 50
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 180, 213
PfeI GAWTC 1 cut(s) 473
PflFI GACNNNGTC 1 cut(s) 484
PkrI GCNGC 6 cut(s) 33, 36, 39, 42, 194, 317
Ppu21I YACGTR 2 cut(s) 466, 566
Psp124BI GAGCTC 1 cut(s) 228
PspN4I GGNNCC 3 cut(s) 196, 333, 416
PspPI GGNCC 1 cut(s) 194
PspXI VCTCGAGB 1 cut(s) 227
PsyI GACNNNGTC 1 cut(s) 484
RsaI GTAC 2 cut(s) 365, 444
RsaNI GTAC 2 cut(s) 364, 443
RseI CAYNNNNRTG 2 cut(s) 600, 623
SacI GAGCTC 1 cut(s) 228
SapI GCTCTTC 1 cut(s) 50
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 6 cut(s) 32, 35, 38, 41, 193, 316
Sau3AI GATC 2 cut(s) 550, 613
Sau96I GGNCC 1 cut(s) 194
ScrFI CCNGG 1 cut(s) 522
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 409
Sfr274I CTCGAG 1 cut(s) 227
SlaI CTCGAG 1 cut(s) 227
SmiMI CAYNNNNRTG 2 cut(s) 600, 623
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 2 cut(s) 308, 706
SspMI CTAG 3 cut(s) 276, 351, 715
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 1 cut(s) 520
StyI CCWWGG 1 cut(s) 275
TaaI ACNGT 1 cut(s) 625
TaiI ACGT 4 cut(s) 219, 384, 468, 568
TaqI TCGA 2 cut(s) 228, 342
TaqII GACCGA 1 cut(s) 386
TasI AATT 2 cut(s) 308, 706
TatI WGTACW 2 cut(s) 363, 442
TauI GCSGC 5 cut(s) 34, 37, 40, 43, 195
TfiI GAWTC 1 cut(s) 473
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 2 cut(s) 520, 630
TseFI GTSAC 2 cut(s) 325, 566
TseI GCWGC 1 cut(s) 315
Tsp45I GTSAC 2 cut(s) 325, 566
TspDTI ATGAA 1 cut(s) 584
TspGWI ACGGA 2 cut(s) 201, 344
TspRI CASTG 2 cut(s) 520, 630
Tth111I GACNNNGTC 1 cut(s) 484
XapI RAATTY 1 cut(s) 308
XcmI CCANNNNNNNNNTGG 1 cut(s) 661
XhoI CTCGAG 1 cut(s) 227
XmaJI CCTAGG 1 cut(s) 275
XspI CTAG 3 cut(s) 276, 351, 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.