pycom348g00030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000348
OG0009190
Physical Location & Seq
Reverse (-)
22457 .. 24844
2388 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom348g00030.1

Sequence Viewer

Length: 1002 bp
ATGAGAGCAACCGCTCTTTTCCTCTCCCCACCACCACTACTCTCCACACCTACCCCTCTCCTCCTCCTCCACTCACTGCACCCACTCCCCTTCCGCTCCCTCAAACCCAATACCCTCCGCAAACACCCCCTTACCCTATCCCCGTCCCCTACCACCCGCTCCTTCCTCGTCCGCGCCGACGACGGCGACGGCGACAGCGCAGGCCCCGACGACTACGACATGGACGACGAAGAGCTCGAGGAAGTCGACAACAAGAAGGACTTCGACATCGAGTACGACACCCTAGCTGCTGCTGCAGCTGCCGCCGCCGCCGCCAATGGCGAAGAGGACATCGCCATGGTGCAGAGCAAGAGCTTCGTGTTCACGCAAGGTTGGGACTCGGAGATGGTGGTGGATTATCGGATTAACGAAGACGAATTTCACAAGATTAGCTTGCTGGACTGCGATTTCTTCATCAGGAAACCTCCCGATCCCGATAATGACGTCTATGATTTCAGAGAGATGTATGTTACTCCTCCGGACACAGATGTTTATGCGATTCCCAGGGTCCTTGCTCCAATGCCTCGGAAGTACATTCGATGTGCGAAGAGTGACTTTAGCTGCTACAATGTAACTGAACCACCCATTGATGCTCCTAGAGATCCATTGTACAAGTCTGAACGGGACGTGTTGAAGGTTTACTTGACAAAGCACTACAGGAACCGGAGGTTGGGGGACCCTGATTTTGTGCTGGATTTTGAGGAGATTTATGTACTTGACTCCAAAACAAAGTCCATTAGCAGAGCTAAAGTTTTGGTCAGTGTTCTGGGGGGAAGAAGCAGAGATAGGAGACACGACCTGCTTGTAATACGTGATAATGGAAACTCCTTCAGAATAGTTCACGGGAGTGAAAAAGATGATCCGACAACAGTGATAGAGAGGGAAGAGTGGAATAAGACCAGACAGGACATGGAAAGGCATCTTAGCAAGCTACGAGATTTCAATGTTTCCAATTGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

334

Amino Acids

38.28

Weight (kDa)

5.04

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 486
Acc36I ACCTGC 1 cut(s) 846
AccBSI CCGCTC 3 cut(s) 14, 96, 159
AccI GTMKAC 1 cut(s) 246
AccII CGCG 1 cut(s) 174
AccIII TCCGGA 1 cut(s) 517
AciI CCGC 9 cut(s) 12, 94, 118, 157, 172, 303, 306, 309, 312
AclWI GGATC 3 cut(s) 464, 635, 893
AcsI RAATTY 1 cut(s) 416
AcuI CTGAAG 1 cut(s) 853
AcyI GRCGYC 1 cut(s) 483
AfaI GTAC 4 cut(s) 275, 572, 650, 753
AfiI CCNNNNNNNGG 1 cut(s) 709
AflIII ACRYGT 1 cut(s) 666
AgsI TTSAA 2 cut(s) 673, 982
AjiI CACGTC 1 cut(s) 667
AjnI CCWGG 1 cut(s) 542
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AleI CACNNNNGTG 1 cut(s) 885
AluBI AGCT 8 cut(s) 235, 287, 299, 354, 432, 600, 785, 970
AluI AGCT 8 cut(s) 235, 287, 299, 354, 432, 600, 785, 970
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 823
AlwI GGATC 3 cut(s) 464, 635, 893
Ama87I CYCGRG 1 cut(s) 236
Aor13HI TCCGGA 1 cut(s) 517
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 6 cut(s) 287, 290, 293, 296, 299, 600
ApoI RAATTY 1 cut(s) 416
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
Asp700I GAANNNNTTC 1 cut(s) 260
AspLEI GCGC 2 cut(s) 176, 200
AspS9I GGNCC 3 cut(s) 203, 547, 715
AvaI CYCGRG 1 cut(s) 236
AvaII GGWCC 2 cut(s) 547, 715
BanII GRGCYC 1 cut(s) 237
BbsI GAAGAC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 6 cut(s) 274, 277, 280, 286, 308, 587
BccI CCATC 1 cut(s) 379
BceAI ACGGC 2 cut(s) 199, 205
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciT130I CCWGG 1 cut(s) 544
BcoDI GTCTC 1 cut(s) 823
BfaI CTAG 3 cut(s) 284, 636, 1000
BfmI CTRYAG 2 cut(s) 294, 694
BfuAI ACCTGC 1 cut(s) 846
Bme1390I CCNGG 1 cut(s) 544
Bme18I GGWCC 2 cut(s) 547, 715
BmeT110I CYCGRG 1 cut(s) 236
BmgBI CACGTC 1 cut(s) 667
BmgT120I GGNCC 3 cut(s) 203, 547, 715
BmiI GGNNCC 5 cut(s) 205, 548, 701, 716, 717
BmrFI CCNGG 1 cut(s) 544
BmsI GCATC 2 cut(s) 619, 967
BpiI GAAGAC 1 cut(s) 417
BsaAI YACGTR 1 cut(s) 851
BsaHI GRCGYC 1 cut(s) 483
BsaJI CCNNGG 4 cut(s) 336, 542, 543, 563
BsaWI WCCGGW 2 cut(s) 517, 702
Bsc4I CCNNNNNNNGG 1 cut(s) 709
BseAI TCCGGA 1 cut(s) 517
BseBI CCWGG 1 cut(s) 544
BseDI CCNNGG 4 cut(s) 336, 542, 543, 563
BseLI CCNNNNNNNGG 1 cut(s) 709
BseRI GAGGAG 5 cut(s) 50, 53, 56, 504, 755
BseXI GCAGC 6 cut(s) 274, 277, 280, 286, 308, 587
BsgI GTGCAG 2 cut(s) 62, 362
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 2 cut(s) 518, 703
BslFI GGGAC 4 cut(s) 130, 389, 677, 728
BslI CCNNNNNNNGG 1 cut(s) 709
BsmAI GTCTC 1 cut(s) 823
BsmFI GGGAC 4 cut(s) 130, 389, 677, 728
BsnI GGCC 1 cut(s) 204
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 237
Bsp13I TCCGGA 1 cut(s) 517
Bsp1407I TGTACA 1 cut(s) 648
Bsp143I GATC 3 cut(s) 469, 640, 898
Bsp19I CCATGG 1 cut(s) 336
BspACI CCGC 9 cut(s) 12, 94, 118, 157, 172, 303, 306, 309, 312
BspANI GGCC 1 cut(s) 204
BspEI TCCGGA 1 cut(s) 517
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 5 cut(s) 205, 548, 701, 716, 717
BspMAI CTGCAG 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 846
BspPI GGATC 3 cut(s) 464, 635, 893
BspQI GCTCTTC 1 cut(s) 225
BsrBI CCGCTC 3 cut(s) 14, 96, 159
BsrGI TGTACA 1 cut(s) 648
BssECI CCNNGG 4 cut(s) 336, 542, 543, 563
BssMI GATC 3 cut(s) 469, 640, 898
BssNI GRCGYC 1 cut(s) 483
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 544
Bst4CI ACNGT 1 cut(s) 910
Bst6I CTCTTC 4 cut(s) 225, 318, 581, 918
BstACI GRCGYC 1 cut(s) 483
BstAUI TGTACA 1 cut(s) 648
BstBAI YACGTR 1 cut(s) 851
BstC8I GCNNGC 3 cut(s) 202, 434, 968
BstDEI CTNAG 1 cut(s) 962
BstDSI CCRYGG 1 cut(s) 336
BstFNI CGCG 1 cut(s) 174
BstHHI GCGC 2 cut(s) 176, 200
BstKTI GATC 3 cut(s) 472, 643, 901
BstMAI GTCTC 1 cut(s) 823
BstMBI GATC 3 cut(s) 469, 640, 898
BstMWI GCNNNNNNNGC 7 cut(s) 293, 296, 299, 302, 305, 308, 311
BstNI CCWGG 1 cut(s) 544
BstSCI CCNGG 1 cut(s) 542
BstSFI CTRYAG 2 cut(s) 294, 694
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 6 cut(s) 274, 277, 280, 286, 308, 587
BstV2I GAAGAC 1 cut(s) 417
BstX2I RGATCY 1 cut(s) 640
BstYI RGATCY 1 cut(s) 640
BsuRI GGCC 1 cut(s) 204
BtgI CCRYGG 1 cut(s) 336
BtgZI GCGATG 1 cut(s) 316
BtrI CACGTC 1 cut(s) 667
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 3 cut(s) 74, 805, 915
BveI ACCTGC 1 cut(s) 846
Cac8I GCNNGC 3 cut(s) 202, 434, 968
CfoI GCGC 2 cut(s) 176, 200
Cfr13I GGNCC 3 cut(s) 203, 547, 715
Csp6I GTAC 4 cut(s) 274, 571, 649, 752
CviAII CATG 3 cut(s) 220, 337, 949
CviJI RGCY 9 cut(s) 204, 235, 287, 299, 354, 432, 600, 785, 970
CviKI_1 RGCY 9 cut(s) 204, 235, 287, 299, 354, 432, 600, 785, 970
CviQI GTAC 4 cut(s) 274, 571, 649, 752
DdeI CTNAG 1 cut(s) 962
DpnI GATC 3 cut(s) 471, 642, 900
DpnII GATC 3 cut(s) 469, 640, 898
Eam1104I CTCTTC 4 cut(s) 225, 318, 581, 918
EarI CTCTTC 4 cut(s) 225, 318, 581, 918
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 2 cut(s) 547, 715
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 853
Eco88I CYCGRG 1 cut(s) 236
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 3 cut(s) 203, 547, 715
EcoRII CCWGG 1 cut(s) 542
EcoT14I CCWWGG 1 cut(s) 336
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 3 cut(s) 223, 340, 952
FaiI YATR 7 cut(s) 221, 338, 489, 507, 534, 750, 950
FalI AAGNNNNNCTT 8 cut(s) 245, 277, 416, 448, 578, 610, 665, 697
FaqI GGGAC 4 cut(s) 130, 389, 677, 728
FatI CATG 3 cut(s) 219, 336, 948
FauI CCCGC 1 cut(s) 164
FblI GTMKAC 1 cut(s) 246
FriOI GRGCYC 1 cut(s) 237
FspBI CTAG 3 cut(s) 284, 636, 1000
GlaI GCGC 2 cut(s) 175, 199
HaeIII GGCC 1 cut(s) 204
HapII CCGG 2 cut(s) 518, 703
HhaI GCGC 2 cut(s) 176, 200
Hin1I GRCGYC 1 cut(s) 483
Hin1II CATG 3 cut(s) 223, 340, 952
Hin6I GCGC 2 cut(s) 174, 198
HinP1I GCGC 2 cut(s) 174, 198
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 3 cut(s) 377, 538, 758
HpaII CCGG 2 cut(s) 518, 703
Hpy166II GTNNAC 4 cut(s) 247, 363, 679, 880
Hpy188I TCNGA 7 cut(s) 382, 402, 497, 567, 658, 872, 903
Hpy188III TCNNGA 4 cut(s) 457, 467, 473, 518
Hpy8I GTNNAC 4 cut(s) 247, 363, 679, 880
Hpy99I CGWCG 5 cut(s) 182, 185, 191, 212, 230
HpyAV CCTTC 5 cut(s) 100, 172, 250, 667, 877
HpyCH4III ACNGT 1 cut(s) 910
HpyCH4IV ACGT 3 cut(s) 483, 666, 850
HpyCH4V TGCA 3 cut(s) 79, 296, 343
HpyF10VI GCNNNNNNNGC 7 cut(s) 293, 296, 299, 302, 305, 308, 311
HpyF3I CTNAG 1 cut(s) 962
HpySE526I ACGT 3 cut(s) 483, 666, 850
Hsp92I GRCGYC 1 cut(s) 483
Hsp92II CATG 3 cut(s) 223, 340, 952
HspAI GCGC 2 cut(s) 174, 198
KflI GGGWCCC 1 cut(s) 715
Kpn2I TCCGGA 1 cut(s) 517
Kzo9I GATC 3 cut(s) 469, 640, 898
LguI GCTCTTC 1 cut(s) 225
LmnI GCTCC 4 cut(s) 101, 164, 559, 637
Lsp1109I GCAGC 6 cut(s) 274, 277, 280, 286, 308, 587
LweI GCATC 2 cut(s) 619, 967
MaeI CTAG 3 cut(s) 284, 636, 1000
MaeII ACGT 3 cut(s) 483, 666, 850
MaeIII GTNAC 3 cut(s) 508, 590, 610
MalI GATC 3 cut(s) 471, 642, 900
MbiI CCGCTC 3 cut(s) 14, 96, 159
MboI GATC 3 cut(s) 469, 640, 898
MboII GAAGA 7 cut(s) 242, 335, 422, 442, 598, 825, 935
MfeI CAATTG 1 cut(s) 991
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 2 cut(s) 416, 991
MlyI GAGTC 2 cut(s) 371, 752
MmeI TCCRAC 1 cut(s) 926
MroI TCCGGA 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 260
MseI TTAA 1 cut(s) 405
MslI CAYNNNNRTG 2 cut(s) 335, 885
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 2 cut(s) 518, 703
MspR9I CCNGG 1 cut(s) 544
MunI CAATTG 1 cut(s) 991
MvaI CCWGG 1 cut(s) 544
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 7 cut(s) 293, 296, 299, 302, 305, 308, 311
NcoI CCATGG 1 cut(s) 336
NdeII GATC 3 cut(s) 469, 640, 898
NlaIII CATG 3 cut(s) 223, 340, 952
NlaIV GGNNCC 5 cut(s) 205, 548, 701, 716, 717
NmuCI GTSAC 1 cut(s) 590
OliI CACNNNNGTG 1 cut(s) 885
PaeR7I CTCGAG 1 cut(s) 236
PasI CCCWGGG 1 cut(s) 543
PciSI GCTCTTC 1 cut(s) 225
PcsI WCGNNNNNNNCGW 5 cut(s) 174, 189, 222, 234, 243
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 1 cut(s) 538
PleI GAGTC 2 cut(s) 371, 752
PpsI GAGTC 2 cut(s) 371, 752
Ppu21I YACGTR 1 cut(s) 851
PpuMI RGGWCCY 2 cut(s) 547, 715
Psp124BI GAGCTC 1 cut(s) 237
Psp5II RGGWCCY 2 cut(s) 547, 715
Psp6I CCWGG 1 cut(s) 542
PspGI CCWGG 1 cut(s) 542
PspN4I GGNNCC 5 cut(s) 205, 548, 701, 716, 717
PspPI GGNCC 3 cut(s) 203, 547, 715
PspPPI RGGWCCY 2 cut(s) 547, 715
PspXI VCTCGAGB 1 cut(s) 236
PstI CTGCAG 1 cut(s) 298
PsuI RGATCY 1 cut(s) 640
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 4 cut(s) 275, 572, 650, 753
RsaNI GTAC 4 cut(s) 274, 571, 649, 752
RseI CAYNNNNRTG 2 cut(s) 335, 885
SacI GAGCTC 1 cut(s) 237
SalI GTCGAC 1 cut(s) 245
SapI GCTCTTC 1 cut(s) 225
SaqAI TTAA 1 cut(s) 405
Sau3AI GATC 3 cut(s) 469, 640, 898
Sau96I GGNCC 3 cut(s) 203, 547, 715
SchI GAGTC 2 cut(s) 371, 752
ScrFI CCNGG 1 cut(s) 544
SduI GDGCHC 1 cut(s) 237
SfaNI GCATC 2 cut(s) 619, 967
SfcI CTRYAG 2 cut(s) 294, 694
Sfr274I CTCGAG 1 cut(s) 236
SinI GGWCC 2 cut(s) 547, 715
SlaI CTCGAG 1 cut(s) 236
SmiMI CAYNNNNRTG 2 cut(s) 335, 885
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
Sse9I AATT 2 cut(s) 416, 991
SsiI CCGC 9 cut(s) 12, 94, 118, 157, 172, 303, 306, 309, 312
SspMI CTAG 3 cut(s) 284, 636, 1000
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 1 cut(s) 542
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 1 cut(s) 910
TaiI ACGT 3 cut(s) 486, 669, 853
TaqI TCGA 5 cut(s) 237, 246, 264, 270, 577
TasI AATT 2 cut(s) 416, 991
TatI WGTACW 3 cut(s) 570, 648, 751
TauI GCSGC 4 cut(s) 305, 308, 311, 314
TfiI GAWTC 1 cut(s) 538
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TscAI CASTG 3 cut(s) 81, 805, 915
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 6 cut(s) 287, 290, 293, 296, 299, 600
Tsp45I GTSAC 1 cut(s) 590
TspDTI ATGAA 1 cut(s) 442
TspRI CASTG 3 cut(s) 81, 805, 915
VpaK11BI GGWCC 2 cut(s) 547, 715
XapI RAATTY 1 cut(s) 416
XcmI CCANNNNNNNNNTGG 1 cut(s) 946
XhoI CTCGAG 1 cut(s) 236
XmiI GTMKAC 1 cut(s) 246
XmnI GAANNNNTTC 1 cut(s) 260
XspI CTAG 3 cut(s) 284, 636, 1000
ZraI GACGTC 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.