Rh6DG377000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0009190
Physical Location & Seq
Forward (+)
57135566 .. 57138382
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG377000.1

Sequence Viewer

Length: 918 bp
ATGAGAGCCACCGCTCTCTTCCTCTCTCCGCCGCCGCCGCCGCCGCTCTTCACCCACAAACCTCTCTTCACACCCACCACACCTCTCCTCCTCCACTCTTCCCACCCCCTCCCTTTCCGCTCCCTCAAACCCAAACCCGCCCTCAACCGCCCCTCCTTCCTCGTCCGCGCCGACGACGGCGACGGCGACGGAGGCGGCCCCGACGACTACGACATGGACGTTGAGGAGCTCGAGGAGGTCGACAACAAGAAGGACTTCGACATCGAGTACGACCCCCTCGCCGGCGCCTCCGCCGCAGTCGACGCCGCCGCCGCCAACGGTGATGTCGACATCGCCATGGTGCCGAGCAAGAGCTTCGTGTTCACGCAGGGCTGGGACTCCGAGATGGTGGTGGATTATCGGATCAACGAGGACGAGTTTCACAAGATTAGTTTGCTCGATTGTGATTTCTTTATTCGGAAACCGCCGGACCCCGATAACGACGTCTTCGATTTCAGAGAGATGTATGTTACTCCTCCGGATACAGATGTTTATGCCATTCCTAGGGTTTTAGCTCCAATGCCTGACAAGTACATTCGATGCGCCCAGAGTGAATTTAGCTGCTACAATGTGACGGAACCGCCCATTGATGCTCCTAGAGACCCATTGTACAAGACCGAGAGGGACGTTTTTAAGGTTTATTTGACAAAGCACTACAGGAACCGGCGCTTGGGAGACCCTGATTTTGTACTGGATTTTGAAGAGATTTATGTGATTGATTCCAAGACAAAGTCTATTAGCAGAGCAAAAGTCCTGGTCAGTGTTCCGGGGGGAAGAACCAGAGATAGAAGACATGATCTGCTTGTAATACGTGACAATGGCAACTCCTTCAGAATAGTTCATGCGGTAACCCACTCTGATCCAACTCTATTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

305

Amino Acids

34.56

Weight (kDa)

4.89

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 486
AccB1I GGYRCC 2 cut(s) 284, 340
AccBSI CCGCTC 3 cut(s) 14, 46, 120
AccI GTMKAC 3 cut(s) 240, 300, 327
AccII CGCG 1 cut(s) 168
AccIII TCCGGA 1 cut(s) 517
AclWI GGATC 2 cut(s) 410, 893
AcsI RAATTY 1 cut(s) 593
AcuI CTGAAG 1 cut(s) 853
AcyI GRCGYC 3 cut(s) 285, 303, 483
AfaI GTAC 4 cut(s) 269, 572, 650, 729
AfiI CCNNNNNNNGG 3 cut(s) 281, 543, 709
AgsI TTSAA 1 cut(s) 740
AjnI CCWGG 1 cut(s) 792
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AluBI AGCT 4 cut(s) 229, 354, 554, 600
AluI AGCT 4 cut(s) 229, 354, 554, 600
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 2 cut(s) 633, 708
AlwI GGATC 2 cut(s) 410, 893
Ama87I CYCGRG 1 cut(s) 230
Aor13HI TCCGGA 1 cut(s) 517
AoxI GGCC 1 cut(s) 196
ApeKI GCWGC 1 cut(s) 600
ApoI RAATTY 1 cut(s) 593
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
Asp700I GAANNNNTTC 1 cut(s) 254
AspA2I CCTAGG 1 cut(s) 542
AspLEI GCGC 4 cut(s) 170, 287, 584, 708
AspS9I GGNCC 2 cut(s) 197, 469
AsuC2I CCSGG 1 cut(s) 807
AsuHPI GGTGA 2 cut(s) 43, 332
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 1 cut(s) 469
AvrII CCTAGG 1 cut(s) 542
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BanI GGYRCC 2 cut(s) 284, 340
BanII GRGCYC 1 cut(s) 231
BbsI GAAGAC 2 cut(s) 478, 835
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 1 cut(s) 587
BccI CCATC 1 cut(s) 379
BceAI ACGGC 2 cut(s) 193, 199
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciT130I CCWGG 1 cut(s) 794
BciVI GTATCC 1 cut(s) 514
BcnI CCSGG 1 cut(s) 807
BcoDI GTCTC 2 cut(s) 633, 708
BfaI CTAG 2 cut(s) 543, 636
BfmI CTRYAG 1 cut(s) 694
BfoI RGCGCY 2 cut(s) 288, 709
BfuI GTATCC 1 cut(s) 514
BlnI CCTAGG 1 cut(s) 542
Bme1390I CCNGG 2 cut(s) 794, 807
Bme18I GGWCC 1 cut(s) 469
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 2 cut(s) 197, 469
BmiI GGNNCC 6 cut(s) 199, 286, 342, 471, 618, 701
BmrFI CCNGG 2 cut(s) 794, 807
BmsI GCATC 2 cut(s) 569, 619
BpiI GAAGAC 2 cut(s) 478, 835
BpuMI CCSGG 1 cut(s) 807
BsaAI YACGTR 1 cut(s) 851
BsaHI GRCGYC 3 cut(s) 285, 303, 483
BsaI GGTCTC 2 cut(s) 633, 708
BsaJI CCNNGG 3 cut(s) 336, 542, 806
BsaWI WCCGGW 1 cut(s) 517
BsaXI ACNNNNNCTCC 4 cut(s) 72, 102, 137, 167
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 543, 709
Bse118I RCCGGY 2 cut(s) 281, 702
Bse1I ACTGG 1 cut(s) 735
BseAI TCCGGA 1 cut(s) 517
BseBI CCWGG 1 cut(s) 794
BseDI CCNNGG 3 cut(s) 336, 542, 806
BseLI CCNNNNNNNGG 3 cut(s) 281, 543, 709
BseNI ACTGG 1 cut(s) 735
BseRI GAGGAG 5 cut(s) 77, 80, 239, 248, 504
BseXI GCAGC 1 cut(s) 587
BseYI CCCAGC 1 cut(s) 372
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 1 cut(s) 198
BshNI GGYRCC 2 cut(s) 284, 340
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 5 cut(s) 282, 467, 518, 703, 806
BslFI GGGAC 2 cut(s) 389, 677
BslI CCNNNNNNNGG 3 cut(s) 281, 543, 709
BsmAI GTCTC 2 cut(s) 633, 708
BsmFI GGGAC 2 cut(s) 389, 677
BsnI GGCC 1 cut(s) 198
Bso31I GGTCTC 2 cut(s) 633, 708
BsoBI CYCGRG 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 231
Bsp13I TCCGGA 1 cut(s) 517
Bsp1407I TGTACA 1 cut(s) 648
Bsp143I GATC 3 cut(s) 402, 835, 898
Bsp19I CCATGG 1 cut(s) 336
BspANI GGCC 1 cut(s) 198
BspEI TCCGGA 1 cut(s) 517
BspFNI CGCG 1 cut(s) 168
BspLI GGNNCC 6 cut(s) 199, 286, 342, 471, 618, 701
BspPI GGATC 2 cut(s) 410, 893
BspQI GCTCTTC 1 cut(s) 53
BspT107I GGYRCC 2 cut(s) 284, 340
BspTNI GGTCTC 2 cut(s) 633, 708
BsrBI CCGCTC 3 cut(s) 14, 46, 120
BsrFI RCCGGY 2 cut(s) 281, 702
BsrGI TGTACA 1 cut(s) 648
BsrI ACTGG 1 cut(s) 735
BssAI RCCGGY 2 cut(s) 281, 702
BssECI CCNNGG 3 cut(s) 336, 542, 806
BssMI GATC 3 cut(s) 402, 835, 898
BssNI GRCGYC 3 cut(s) 285, 303, 483
BssT1I CCWWGG 2 cut(s) 336, 542
Bst2UI CCWGG 1 cut(s) 794
Bst4CI ACNGT 1 cut(s) 320
Bst6I CTCTTC 5 cut(s) 23, 53, 71, 103, 735
BstACI GRCGYC 3 cut(s) 285, 303, 483
BstAUI TGTACA 1 cut(s) 648
BstBAI YACGTR 1 cut(s) 851
BstC8I GCNNGC 1 cut(s) 283
BstDSI CCRYGG 1 cut(s) 336
BstEII GGTNACC 1 cut(s) 886
BstFNI CGCG 1 cut(s) 168
BstH2I RGCGCY 2 cut(s) 288, 709
BstHHI GCGC 4 cut(s) 170, 287, 584, 708
BstKTI GATC 3 cut(s) 405, 838, 901
BstMAI GTCTC 2 cut(s) 633, 708
BstMBI GATC 3 cut(s) 402, 835, 898
BstMWI GCNNNNNNNGC 7 cut(s) 37, 40, 43, 192, 293, 302, 311
BstNI CCWGG 1 cut(s) 794
BstPI GGTNACC 1 cut(s) 886
BstSCI CCNGG 2 cut(s) 792, 805
BstSFI CTRYAG 1 cut(s) 694
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 587
BstV2I GAAGAC 2 cut(s) 478, 835
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 336
BtgZI GCGATG 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 805
Cac8I GCNNGC 1 cut(s) 283
CfoI GCGC 4 cut(s) 170, 287, 584, 708
Cfr10I RCCGGY 2 cut(s) 281, 702
Cfr13I GGNCC 2 cut(s) 197, 469
CseI GACGC 1 cut(s) 311
Csp6I GTAC 4 cut(s) 268, 571, 649, 728
CviAII CATG 4 cut(s) 214, 337, 833, 881
CviJI RGCY 7 cut(s) 8, 198, 229, 354, 372, 554, 600
CviKI_1 RGCY 7 cut(s) 8, 198, 229, 354, 372, 554, 600
CviQI GTAC 4 cut(s) 268, 571, 649, 728
DinI GGCGCC 1 cut(s) 286
DpnI GATC 3 cut(s) 404, 837, 900
DpnII GATC 3 cut(s) 402, 835, 898
Eam1104I CTCTTC 5 cut(s) 23, 53, 71, 103, 735
EarI CTCTTC 5 cut(s) 23, 53, 71, 103, 735
EciI GGCGGA 2 cut(s) 18, 280
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 2 cut(s) 336, 542
Eco24I GRGCYC 1 cut(s) 231
Eco31I GGTCTC 2 cut(s) 633, 708
Eco47I GGWCC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 853
Eco88I CYCGRG 1 cut(s) 230
Eco91I GGTNACC 1 cut(s) 886
EcoICRI GAGCTC 1 cut(s) 229
EcoO65I GGTNACC 1 cut(s) 886
EcoRII CCWGG 1 cut(s) 792
EcoT14I CCWWGG 2 cut(s) 336, 542
EcoT38I GRGCYC 1 cut(s) 231
EgeI GGCGCC 1 cut(s) 286
EheI GGCGCC 1 cut(s) 286
ErhI CCWWGG 2 cut(s) 336, 542
FaeI CATG 4 cut(s) 217, 340, 836, 884
FaiI YATR 7 cut(s) 215, 338, 507, 534, 750, 834, 882
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 2 cut(s) 389, 677
FatI CATG 4 cut(s) 213, 336, 832, 880
FauI CCCGC 1 cut(s) 145
FblI GTMKAC 3 cut(s) 240, 300, 327
FriOI GRGCYC 1 cut(s) 231
FspBI CTAG 2 cut(s) 543, 636
GlaI GCGC 4 cut(s) 169, 286, 583, 707
GsaI CCCAGC 1 cut(s) 376
HaeII RGCGCY 2 cut(s) 288, 709
HaeIII GGCC 1 cut(s) 198
HapII CCGG 5 cut(s) 282, 467, 518, 703, 806
HgaI GACGC 1 cut(s) 311
HhaI GCGC 4 cut(s) 170, 287, 584, 708
Hin1I GRCGYC 3 cut(s) 285, 303, 483
Hin1II CATG 4 cut(s) 217, 340, 836, 884
Hin6I GCGC 4 cut(s) 168, 285, 582, 706
HinP1I GCGC 4 cut(s) 168, 285, 582, 706
HincII GTYRAC 3 cut(s) 241, 301, 328
HindII GTYRAC 3 cut(s) 241, 301, 328
HinfI GANTC 2 cut(s) 377, 758
HpaII CCGG 5 cut(s) 282, 467, 518, 703, 806
HphI GGTGA 2 cut(s) 43, 332
Hpy166II GTNNAC 4 cut(s) 241, 301, 328, 363
Hpy188I TCNGA 6 cut(s) 382, 402, 459, 497, 872, 898
Hpy188III TCNNGA 1 cut(s) 518
Hpy8I GTNNAC 4 cut(s) 241, 301, 328, 363
Hpy99I CGWCG 7 cut(s) 176, 179, 185, 191, 206, 305, 485
HpyAV CCTTC 3 cut(s) 166, 244, 877
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 4 cut(s) 219, 483, 666, 850
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 40, 43, 192, 293, 302, 311
HpySE526I ACGT 4 cut(s) 219, 483, 666, 850
Hsp92I GRCGYC 3 cut(s) 285, 303, 483
Hsp92II CATG 4 cut(s) 217, 340, 836, 884
HspAI GCGC 4 cut(s) 168, 285, 582, 706
KasI GGCGCC 1 cut(s) 284
Kpn2I TCCGGA 1 cut(s) 517
KroI GCCGGC 1 cut(s) 281
KroNI GCCGGC 1 cut(s) 283
Kzo9I GATC 3 cut(s) 402, 835, 898
LguI GCTCTTC 1 cut(s) 53
LmnI GCTCC 4 cut(s) 125, 226, 559, 637
Lsp1109I GCAGC 1 cut(s) 587
LweI GCATC 2 cut(s) 569, 619
MaeI CTAG 2 cut(s) 543, 636
MaeII ACGT 4 cut(s) 219, 483, 666, 850
MaeIII GTNAC 4 cut(s) 508, 610, 851, 886
MalI GATC 3 cut(s) 404, 837, 900
MbiI CCGCTC 3 cut(s) 14, 46, 120
MboI GATC 3 cut(s) 402, 835, 898
MboII GAAGA 8 cut(s) 10, 40, 58, 90, 478, 752, 825, 840
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 1 cut(s) 593
Mly113I GGCGCC 1 cut(s) 285
MlyI GAGTC 1 cut(s) 371
MreI CGCCGGCG 1 cut(s) 281
MroI TCCGGA 1 cut(s) 517
MroNI GCCGGC 1 cut(s) 281
MroXI GAANNNNTTC 1 cut(s) 254
MseI TTAA 2 cut(s) 672, 916
MslI CAYNNNNRTG 1 cut(s) 335
MspI CCGG 5 cut(s) 282, 467, 518, 703, 806
MspR9I CCNGG 2 cut(s) 794, 807
MvaI CCWGG 1 cut(s) 794
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 7 cut(s) 37, 40, 43, 192, 293, 302, 311
NaeI GCCGGC 1 cut(s) 283
NarI GGCGCC 1 cut(s) 285
NciI CCSGG 1 cut(s) 807
NcoI CCATGG 1 cut(s) 336
NdeII GATC 3 cut(s) 402, 835, 898
NgoMIV GCCGGC 1 cut(s) 281
NlaIII CATG 4 cut(s) 217, 340, 836, 884
NlaIV GGNNCC 6 cut(s) 199, 286, 342, 471, 618, 701
NmeAIII GCCGAG 1 cut(s) 369
NmuCI GTSAC 2 cut(s) 610, 851
PaeR7I CTCGAG 1 cut(s) 230
PciSI GCTCTTC 1 cut(s) 53
PcsI WCGNNNNNNNCGW 6 cut(s) 168, 183, 216, 237, 324, 486
PdiI GCCGGC 1 cut(s) 283
PdmI GAANNNNTTC 1 cut(s) 254
PfeI GAWTC 1 cut(s) 758
PflFI GACNNNGTC 1 cut(s) 769
PleI GAGTC 1 cut(s) 371
PluTI GGCGCC 1 cut(s) 288
PpsI GAGTC 1 cut(s) 371
Ppu21I YACGTR 1 cut(s) 851
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 792
PspEI GGTNACC 1 cut(s) 886
PspFI CCCAGC 1 cut(s) 372
PspGI CCWGG 1 cut(s) 792
PspN4I GGNNCC 6 cut(s) 199, 286, 342, 471, 618, 701
PspPI GGNCC 2 cut(s) 197, 469
PspXI VCTCGAGB 1 cut(s) 230
PsyI GACNNNGTC 1 cut(s) 769
RsaI GTAC 4 cut(s) 269, 572, 650, 729
RsaNI GTAC 4 cut(s) 268, 571, 649, 728
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 1 cut(s) 231
SalI GTCGAC 3 cut(s) 239, 299, 326
SapI GCTCTTC 1 cut(s) 53
SaqAI TTAA 2 cut(s) 672, 916
Sau3AI GATC 3 cut(s) 402, 835, 898
Sau96I GGNCC 2 cut(s) 197, 469
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 2 cut(s) 794, 807
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 2 cut(s) 569, 619
SfcI CTRYAG 1 cut(s) 694
SfoI GGCGCC 1 cut(s) 286
Sfr274I CTCGAG 1 cut(s) 230
SgrAI CRCCGGYG 1 cut(s) 281
SinI GGWCC 1 cut(s) 469
SlaI CTCGAG 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 1 cut(s) 593
SspDI GGCGCC 1 cut(s) 284
SspMI CTAG 2 cut(s) 543, 636
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 792, 805
StyI CCWWGG 2 cut(s) 336, 542
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 4 cut(s) 222, 486, 669, 853
TaqI TCGA 9 cut(s) 231, 240, 258, 264, 300, 327, 438, 489, 577
TaqII GACCGA 1 cut(s) 671
TasI AATT 1 cut(s) 593
TatI WGTACW 3 cut(s) 570, 648, 727
TfiI GAWTC 1 cut(s) 758
Tru1I TTAA 2 cut(s) 672, 916
Tru9I TTAA 2 cut(s) 672, 916
TscAI CASTG 1 cut(s) 805
TseFI GTSAC 2 cut(s) 610, 851
TseI GCWGC 1 cut(s) 600
Tsp45I GTSAC 2 cut(s) 610, 851
TspDTI ATGAA 1 cut(s) 869
TspGWI ACGGA 2 cut(s) 204, 629
TspRI CASTG 1 cut(s) 805
Tth111I GACNNNGTC 1 cut(s) 769
VpaK11BI GGWCC 1 cut(s) 469
XapI RAATTY 1 cut(s) 593
XhoI CTCGAG 1 cut(s) 230
XmaJI CCTAGG 1 cut(s) 542
XmiI GTMKAC 3 cut(s) 240, 300, 327
XmnI GAANNNNTTC 1 cut(s) 254
XspI CTAG 2 cut(s) 543, 636
ZraI GACGTC 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.