Rh6AG376200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0009190
Physical Location & Seq
Forward (+)
57496472 .. 57499304
2833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG376200.1

Sequence Viewer

Length: 909 bp
ATGAGAGCCACCGCTCTCTTCCTCCCTCCGCCGCCGCCGCCATCCTTCACCCACAAACCTCTCTTCACACCCACCACACCTCTCCTCCTCCACTCTTCCCACCCCCTCCCTTTCCGCTCCCTCAAACCCAAACCCGCCCTCAACCGCCCCTCCTTCCTCGTCCGCGCCGACGACGGCGACGCCGACGGAGGCGGCCCCGACGACTACGACATGGACGTTGAGGAGCTGGAGGAGGTCGACAACAAGAAGGACTTCGACATCGAGTACGACCCCCTCGCCGGCGCCTCCGCCGCAGTCGACGCCGCCGACGGTGATGTCGACATCGCCATGGTGCCGAGCAAGAGCTTCGTGTTCACGCAGGGCTGGGACTCCGAGATGGTGGTGGATTATCGGATCAACGAGGACGAGTTTCACAAGATTAGTTTGCTCGATTGCGATTTCTTTATACGGAAACCGCCGGACCCCGATAACGACGTCTTCGATTTCAGAGAGATGTATGTTACTCCTCCGGATACAGATGTTTATGCCATTCCTAGGGTTTTAGCTCCAATGCCTGACAAGTACATTCGATGCGCCCAGAGTGAATTTAGCTGCTACAATGTGACGGAACCGCCCATTGATGCTCCTAGAGACCCGTTGTACAAGACCGAGAGGGACGTTTTTAAGGTTTATTTGACAAAGCACTACAGGAACCGGCGCTTGGGAGACCCTGATTTTGTACTGGATTTTGAAGAGATTTACGTGATTGATTCCAAGACAAAGTCTATTAGCAGAGCAAAAGTCCTGGTCAGTGTTCCGGGGGGAAGAACCAGAGATAGAAGACATGATCTGCTTGTAATACGTGACAATGGCAACTCCTTCAGAATAGTTCATGCGGTAACCCACTCTGATCCAACTCGATATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

302

Amino Acids

34.38

Weight (kDa)

4.9

Isoelectric Point (pI)

40.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 314
AatII GACGTC 1 cut(s) 477
AccB1I GGYRCC 2 cut(s) 281, 331
AccBSI CCGCTC 2 cut(s) 14, 117
AccI GTMKAC 3 cut(s) 237, 297, 318
AccII CGCG 1 cut(s) 165
AccIII TCCGGA 1 cut(s) 508
AclWI GGATC 2 cut(s) 401, 884
AcsI RAATTY 1 cut(s) 584
AcuI CTGAAG 1 cut(s) 844
AcyI GRCGYC 4 cut(s) 180, 282, 300, 474
AfaI GTAC 4 cut(s) 266, 563, 641, 720
AfiI CCNNNNNNNGG 3 cut(s) 278, 534, 700
AgsI TTSAA 1 cut(s) 731
AjnI CCWGG 1 cut(s) 783
AjuI GAANNNNNNNTTGG 2 cut(s) 683, 715
AluBI AGCT 4 cut(s) 226, 345, 545, 591
AluI AGCT 4 cut(s) 226, 345, 545, 591
Alw26I GTCTC 2 cut(s) 624, 699
AlwI GGATC 2 cut(s) 401, 884
Aor13HI TCCGGA 1 cut(s) 508
AoxI GGCC 1 cut(s) 193
ApeKI GCWGC 1 cut(s) 591
ApoI RAATTY 1 cut(s) 584
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
Asp700I GAANNNNTTC 1 cut(s) 251
AspA2I CCTAGG 1 cut(s) 533
AspLEI GCGC 4 cut(s) 167, 284, 575, 699
AspS9I GGNCC 2 cut(s) 194, 460
AsuC2I CCSGG 1 cut(s) 798
AsuHPI GGTGA 2 cut(s) 40, 323
AvaII GGWCC 1 cut(s) 460
AvrII CCTAGG 1 cut(s) 533
BaeI ACNNNNGTAYC 2 cut(s) 504, 537
BanI GGYRCC 2 cut(s) 281, 331
BbsI GAAGAC 2 cut(s) 469, 826
BbvI GCAGC 1 cut(s) 578
BccI CCATC 2 cut(s) 49, 370
BceAI ACGGC 1 cut(s) 190
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 1 cut(s) 785
BciVI GTATCC 1 cut(s) 505
BcnI CCSGG 1 cut(s) 798
BcoDI GTCTC 2 cut(s) 624, 699
BfaI CTAG 2 cut(s) 534, 627
BfmI CTRYAG 1 cut(s) 685
BfoI RGCGCY 2 cut(s) 285, 700
BfuI GTATCC 1 cut(s) 505
BisI GCNGC 7 cut(s) 32, 35, 38, 193, 291, 303, 592
BlnI CCTAGG 1 cut(s) 533
BlsI GCNGC 7 cut(s) 33, 36, 39, 194, 292, 304, 593
Bme1390I CCNGG 2 cut(s) 785, 798
Bme18I GGWCC 1 cut(s) 460
BmgT120I GGNCC 2 cut(s) 194, 460
BmiI GGNNCC 6 cut(s) 196, 283, 333, 462, 609, 692
BmrFI CCNGG 2 cut(s) 785, 798
BmsI GCATC 2 cut(s) 560, 610
BpiI GAAGAC 2 cut(s) 469, 826
BpmI CTGGAG 1 cut(s) 248
BpuMI CCSGG 1 cut(s) 798
BsaAI YACGTR 2 cut(s) 742, 842
BsaHI GRCGYC 4 cut(s) 180, 282, 300, 474
BsaI GGTCTC 2 cut(s) 624, 699
BsaJI CCNNGG 3 cut(s) 327, 533, 797
BsaWI WCCGGW 1 cut(s) 508
BsaXI ACNNNNNCTCC 4 cut(s) 69, 99, 134, 164
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 534, 700
Bse118I RCCGGY 2 cut(s) 278, 693
Bse1I ACTGG 1 cut(s) 726
BseAI TCCGGA 1 cut(s) 508
BseBI CCWGG 1 cut(s) 785
BseDI CCNNGG 3 cut(s) 327, 533, 797
BseGI GGATG 1 cut(s) 41
BseLI CCNNNNNNNGG 3 cut(s) 278, 534, 700
BseNI ACTGG 1 cut(s) 726
BseRI GAGGAG 5 cut(s) 74, 77, 236, 245, 495
BseXI GCAGC 1 cut(s) 578
BseYI CCCAGC 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 165
BshFI GGCC 1 cut(s) 195
BshNI GGYRCC 2 cut(s) 281, 331
BsiSI CCGG 5 cut(s) 279, 458, 509, 694, 797
BslFI GGGAC 2 cut(s) 380, 668
BslI CCNNNNNNNGG 3 cut(s) 278, 534, 700
BsmAI GTCTC 2 cut(s) 624, 699
BsmFI GGGAC 2 cut(s) 380, 668
BsnI GGCC 1 cut(s) 195
Bso31I GGTCTC 2 cut(s) 624, 699
Bsp13I TCCGGA 1 cut(s) 508
Bsp1407I TGTACA 1 cut(s) 639
Bsp143I GATC 3 cut(s) 393, 826, 889
Bsp19I CCATGG 1 cut(s) 327
BspANI GGCC 1 cut(s) 195
BspEI TCCGGA 1 cut(s) 508
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 6 cut(s) 196, 283, 333, 462, 609, 692
BspPI GGATC 2 cut(s) 401, 884
BspT107I GGYRCC 2 cut(s) 281, 331
BspTNI GGTCTC 2 cut(s) 624, 699
BsrBI CCGCTC 2 cut(s) 14, 117
BsrFI RCCGGY 2 cut(s) 278, 693
BsrGI TGTACA 1 cut(s) 639
BsrI ACTGG 1 cut(s) 726
BssAI RCCGGY 2 cut(s) 278, 693
BssECI CCNNGG 3 cut(s) 327, 533, 797
BssMI GATC 3 cut(s) 393, 826, 889
BssNI GRCGYC 4 cut(s) 180, 282, 300, 474
BssT1I CCWWGG 2 cut(s) 327, 533
Bst2UI CCWGG 1 cut(s) 785
Bst4CI ACNGT 1 cut(s) 311
Bst6I CTCTTC 4 cut(s) 23, 68, 100, 726
BstACI GRCGYC 4 cut(s) 180, 282, 300, 474
BstAUI TGTACA 1 cut(s) 639
BstBAI YACGTR 2 cut(s) 742, 842
BstC8I GCNNGC 1 cut(s) 280
BstDSI CCRYGG 1 cut(s) 327
BstEII GGTNACC 1 cut(s) 877
BstF5I GGATG 1 cut(s) 41
BstFNI CGCG 1 cut(s) 165
BstH2I RGCGCY 2 cut(s) 285, 700
BstHHI GCGC 4 cut(s) 167, 284, 575, 699
BstKTI GATC 3 cut(s) 396, 829, 892
BstMAI GTCTC 2 cut(s) 624, 699
BstMBI GATC 3 cut(s) 393, 826, 889
BstMWI GCNNNNNNNGC 3 cut(s) 37, 290, 299
BstNI CCWGG 1 cut(s) 785
BstPI GGTNACC 1 cut(s) 877
BstSCI CCNGG 2 cut(s) 783, 796
BstSFI CTRYAG 1 cut(s) 685
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 1 cut(s) 578
BstV2I GAAGAC 2 cut(s) 469, 826
BsuI GTATCC 1 cut(s) 505
BsuRI GGCC 1 cut(s) 195
BtgI CCRYGG 1 cut(s) 327
BtgZI GCGATG 1 cut(s) 307
BtsCI GGATG 1 cut(s) 41
BtsIMutI CAGTG 1 cut(s) 796
Cac8I GCNNGC 1 cut(s) 280
CfoI GCGC 4 cut(s) 167, 284, 575, 699
Cfr10I RCCGGY 2 cut(s) 278, 693
Cfr13I GGNCC 2 cut(s) 194, 460
CseI GACGC 2 cut(s) 188, 308
Csp6I GTAC 4 cut(s) 265, 562, 640, 719
CviAII CATG 4 cut(s) 211, 328, 824, 872
CviJI RGCY 7 cut(s) 8, 195, 226, 345, 363, 545, 591
CviKI_1 RGCY 7 cut(s) 8, 195, 226, 345, 363, 545, 591
CviQI GTAC 4 cut(s) 265, 562, 640, 719
DinI GGCGCC 1 cut(s) 283
DpnI GATC 3 cut(s) 395, 828, 891
DpnII GATC 3 cut(s) 393, 826, 889
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
Eam1104I CTCTTC 4 cut(s) 23, 68, 100, 726
EarI CTCTTC 4 cut(s) 23, 68, 100, 726
EciI GGCGGA 2 cut(s) 18, 277
Eco130I CCWWGG 2 cut(s) 327, 533
Eco31I GGTCTC 2 cut(s) 624, 699
Eco32I GATATC 1 cut(s) 902
Eco47I GGWCC 1 cut(s) 460
Eco57I CTGAAG 1 cut(s) 844
Eco91I GGTNACC 1 cut(s) 877
EcoO65I GGTNACC 1 cut(s) 877
EcoRII CCWGG 1 cut(s) 783
EcoRV GATATC 1 cut(s) 902
EcoT14I CCWWGG 2 cut(s) 327, 533
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
ErhI CCWWGG 2 cut(s) 327, 533
FaeI CATG 4 cut(s) 214, 331, 827, 875
FaiI YATR 7 cut(s) 212, 329, 446, 498, 525, 825, 873
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 2 cut(s) 380, 668
FatI CATG 4 cut(s) 210, 327, 823, 871
FauI CCCGC 1 cut(s) 142
FblI GTMKAC 3 cut(s) 237, 297, 318
Fnu4HI GCNGC 7 cut(s) 32, 35, 38, 193, 291, 303, 592
FokI GGATG 1 cut(s) 28
Fsp4HI GCNGC 7 cut(s) 32, 35, 38, 193, 291, 303, 592
FspBI CTAG 2 cut(s) 534, 627
GlaI GCGC 4 cut(s) 166, 283, 574, 698
GluI GCNGC 7 cut(s) 32, 35, 38, 193, 291, 303, 592
GsaI CCCAGC 1 cut(s) 367
GsuI CTGGAG 1 cut(s) 248
HaeII RGCGCY 2 cut(s) 285, 700
HaeIII GGCC 1 cut(s) 195
HapII CCGG 5 cut(s) 279, 458, 509, 694, 797
HgaI GACGC 2 cut(s) 188, 308
HhaI GCGC 4 cut(s) 167, 284, 575, 699
Hin1I GRCGYC 4 cut(s) 180, 282, 300, 474
Hin1II CATG 4 cut(s) 214, 331, 827, 875
Hin6I GCGC 4 cut(s) 165, 282, 573, 697
HinP1I GCGC 4 cut(s) 165, 282, 573, 697
HincII GTYRAC 3 cut(s) 238, 298, 319
HindII GTYRAC 3 cut(s) 238, 298, 319
HinfI GANTC 2 cut(s) 368, 749
HpaII CCGG 5 cut(s) 279, 458, 509, 694, 797
HphI GGTGA 2 cut(s) 40, 323
Hpy166II GTNNAC 4 cut(s) 238, 298, 319, 354
Hpy188I TCNGA 5 cut(s) 373, 393, 488, 863, 889
Hpy188III TCNNGA 1 cut(s) 509
Hpy8I GTNNAC 4 cut(s) 238, 298, 319, 354
Hpy99I CGWCG 8 cut(s) 173, 176, 182, 188, 203, 302, 311, 476
HpyAV CCTTC 4 cut(s) 55, 163, 241, 868
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4IV ACGT 5 cut(s) 216, 474, 657, 741, 841
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 290, 299
HpySE526I ACGT 5 cut(s) 216, 474, 657, 741, 841
Hsp92I GRCGYC 4 cut(s) 180, 282, 300, 474
Hsp92II CATG 4 cut(s) 214, 331, 827, 875
HspAI GCGC 4 cut(s) 165, 282, 573, 697
KasI GGCGCC 1 cut(s) 281
Kpn2I TCCGGA 1 cut(s) 508
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Kzo9I GATC 3 cut(s) 393, 826, 889
LmnI GCTCC 4 cut(s) 122, 223, 550, 628
Lsp1109I GCAGC 1 cut(s) 578
LweI GCATC 2 cut(s) 560, 610
MaeI CTAG 2 cut(s) 534, 627
MaeII ACGT 5 cut(s) 216, 474, 657, 741, 841
MaeIII GTNAC 4 cut(s) 499, 601, 842, 877
MalI GATC 3 cut(s) 395, 828, 891
MbiI CCGCTC 2 cut(s) 14, 117
MboI GATC 3 cut(s) 393, 826, 889
MboII GAAGA 7 cut(s) 10, 55, 87, 469, 743, 816, 831
MluCI AATT 1 cut(s) 584
Mly113I GGCGCC 1 cut(s) 282
MlyI GAGTC 1 cut(s) 362
MreI CGCCGGCG 1 cut(s) 278
MroI TCCGGA 1 cut(s) 508
MroNI GCCGGC 1 cut(s) 278
MroXI GAANNNNTTC 1 cut(s) 251
MseI TTAA 2 cut(s) 663, 907
MslI CAYNNNNRTG 1 cut(s) 326
MspI CCGG 5 cut(s) 279, 458, 509, 694, 797
MspR9I CCNGG 2 cut(s) 785, 798
MvaI CCWGG 1 cut(s) 785
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 3 cut(s) 37, 290, 299
NaeI GCCGGC 1 cut(s) 280
NarI GGCGCC 1 cut(s) 282
NciI CCSGG 1 cut(s) 798
NcoI CCATGG 1 cut(s) 327
NdeII GATC 3 cut(s) 393, 826, 889
NgoMIV GCCGGC 1 cut(s) 278
NlaIII CATG 4 cut(s) 214, 331, 827, 875
NlaIV GGNNCC 6 cut(s) 196, 283, 333, 462, 609, 692
NmeAIII GCCGAG 1 cut(s) 360
NmuCI GTSAC 2 cut(s) 601, 842
PcsI WCGNNNNNNNCGW 6 cut(s) 165, 180, 213, 303, 315, 477
PdiI GCCGGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 1 cut(s) 749
PflFI GACNNNGTC 1 cut(s) 760
PkrI GCNGC 7 cut(s) 33, 36, 39, 194, 292, 304, 593
PleI GAGTC 1 cut(s) 362
PluTI GGCGCC 1 cut(s) 285
PpsI GAGTC 1 cut(s) 362
Ppu21I YACGTR 2 cut(s) 742, 842
Psp6I CCWGG 1 cut(s) 783
PspEI GGTNACC 1 cut(s) 877
PspFI CCCAGC 1 cut(s) 363
PspGI CCWGG 1 cut(s) 783
PspN4I GGNNCC 6 cut(s) 196, 283, 333, 462, 609, 692
PspPI GGNCC 2 cut(s) 194, 460
PsyI GACNNNGTC 1 cut(s) 760
RsaI GTAC 4 cut(s) 266, 563, 641, 720
RsaNI GTAC 4 cut(s) 265, 562, 640, 719
RseI CAYNNNNRTG 1 cut(s) 326
SalI GTCGAC 3 cut(s) 236, 296, 317
SaqAI TTAA 2 cut(s) 663, 907
SatI GCNGC 7 cut(s) 32, 35, 38, 193, 291, 303, 592
Sau3AI GATC 3 cut(s) 393, 826, 889
Sau96I GGNCC 2 cut(s) 194, 460
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 2 cut(s) 785, 798
SfaNI GCATC 2 cut(s) 560, 610
SfcI CTRYAG 1 cut(s) 685
SfoI GGCGCC 1 cut(s) 283
SgrAI CRCCGGYG 1 cut(s) 278
SinI GGWCC 1 cut(s) 460
SmiMI CAYNNNNRTG 1 cut(s) 326
Sse9I AATT 1 cut(s) 584
SspDI GGCGCC 1 cut(s) 281
SspMI CTAG 2 cut(s) 534, 627
StyD4I CCNGG 2 cut(s) 783, 796
StyI CCWWGG 2 cut(s) 327, 533
TaaI ACNGT 1 cut(s) 311
TaiI ACGT 5 cut(s) 219, 477, 660, 744, 844
TaqI TCGA 9 cut(s) 237, 255, 261, 297, 318, 429, 480, 568, 898
TaqII GACCGA 1 cut(s) 662
TasI AATT 1 cut(s) 584
TatI WGTACW 3 cut(s) 561, 639, 718
TauI GCSGC 6 cut(s) 34, 37, 40, 195, 293, 305
TfiI GAWTC 1 cut(s) 749
Tru1I TTAA 2 cut(s) 663, 907
Tru9I TTAA 2 cut(s) 663, 907
TscAI CASTG 1 cut(s) 796
TseFI GTSAC 2 cut(s) 601, 842
TseI GCWGC 1 cut(s) 591
Tsp45I GTSAC 2 cut(s) 601, 842
TspDTI ATGAA 1 cut(s) 860
TspGWI ACGGA 3 cut(s) 201, 463, 620
TspRI CASTG 1 cut(s) 796
Tth111I GACNNNGTC 1 cut(s) 760
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 1 cut(s) 584
XmaJI CCTAGG 1 cut(s) 533
XmiI GTMKAC 3 cut(s) 237, 297, 318
XmnI GAANNNNTTC 1 cut(s) 251
XspI CTAG 2 cut(s) 534, 627
ZraI GACGTC 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.