MD08G1159400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009190
Physical Location & Seq
Forward (+)
18059627 .. 18063915
4289 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1159400.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGAGAGCAACCGCTCTCTTCCTCTCCCCACCACCACTACTCTCCACACCTACCCCTCTCCTCCTCCTCCACTCACTGCACCCACTCCCCTTCCGCTCCCTCAAACCCAATCCCCTCCGCAAACACCCCCTTACGCTATCCCCCTCCCCAACCACCCCCTCCTTCCTCGTCCGCGCCGACGACGGCGACGGCGACAGCGCAGGCCCCGACGACTACGACATGGACGACGAAGAGCTCGAGGAAGTCGACAACAAGAAGGACTTCGACATTCAGTACGATACCCTAGCTGCCGCTGCAGCTGCCGCCGCCGCCGCCGCCAATGGCGAAGAGGACATCGCCATGGTGCAGAGCAAGAGCTTCGTGTTCACGCAAGGTTGGGACTCGGAGATGGTGGTGGATTATCGGATTAACGAAGACGAATTTCACAAGATTAGCTTGCTGGACTGCGATTTCTTCATCAGAAAACCTCCCGATCCCGATAATGACGTCTATGACTTCAGAGAGATGTATGTTACTCCTCCGGACACAGATGTTTATGCGATTCCCAGGGTTCTTGCTCCAATGCCTCAGAAGTACATTCGATGTGCGAAGAGTGACTTTAGCTGCTACAATGTAACGGAACCACCCATTGATGCTCCTAGAGATCCATTGTACAAGTCTGAACGGGACGTGTTGAAGGTTTACTTGACAAAGCACTACAGGAACCGGAGGTTGGGGGACCCTGATTTTTTGCTGGATTTTGAGGAGATTTATGTAATTGACTCCAAAACAAAGTCCATTAGCAGAGCTAAAGTTTTGGTCAGTGTTCTGGGGGGAAGAAGCAGAGATAGAAGACATGATCTGCTTGTAATACGTGATAATGGAAACTCCTTCAGAATAGTTCACGGGAGTGAAAAAGATGATCCCACAACAGTGATAGAGAGGGAAGAGTGGAATAAGACCAGACAGGACATGGAGAGGCATCTTAGCAAGCTACGAGATTTCAATGTTTCCAATTGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

335

Amino Acids

38.27

Weight (kDa)

4.97

Isoelectric Point (pI)

43.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 489
AccBSI CCGCTC 2 cut(s) 14, 96
AccI GTMKAC 1 cut(s) 246
AccII CGCG 1 cut(s) 174
AccIII TCCGGA 1 cut(s) 520
AclWI GGATC 3 cut(s) 467, 638, 896
AcsI RAATTY 1 cut(s) 419
AcuI CTGAAG 2 cut(s) 481, 856
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 3 cut(s) 275, 575, 653
AfiI CCNNNNNNNGG 1 cut(s) 712
AflIII ACRYGT 1 cut(s) 669
AgsI TTSAA 2 cut(s) 676, 985
AjiI CACGTC 1 cut(s) 670
AjnI CCWGG 1 cut(s) 545
AjuI GAANNNNNNNTTGG 2 cut(s) 695, 727
AleI CACNNNNGTG 2 cut(s) 888, 911
AluBI AGCT 8 cut(s) 235, 287, 299, 357, 435, 603, 788, 973
AluI AGCT 8 cut(s) 235, 287, 299, 357, 435, 603, 788, 973
Alw21I GWGCWC 1 cut(s) 237
AlwI GGATC 3 cut(s) 467, 638, 896
Ama87I CYCGRG 1 cut(s) 236
Aor13HI TCCGGA 1 cut(s) 520
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 5 cut(s) 287, 293, 296, 299, 603
ApoI RAATTY 1 cut(s) 419
Asp700I GAANNNNTTC 1 cut(s) 260
AspLEI GCGC 2 cut(s) 176, 200
AspS9I GGNCC 2 cut(s) 203, 718
AvaI CYCGRG 1 cut(s) 236
AvaII GGWCC 1 cut(s) 718
BanII GRGCYC 1 cut(s) 237
BbsI GAAGAC 2 cut(s) 420, 838
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 5 cut(s) 274, 280, 286, 308, 590
BccI CCATC 1 cut(s) 382
BceAI ACGGC 2 cut(s) 199, 205
BcgI CGANNNNNNTGC 2 cut(s) 340, 374
BciT130I CCWGG 1 cut(s) 547
BfaI CTAG 3 cut(s) 284, 639, 1003
BfmI CTRYAG 2 cut(s) 294, 697
Bme1390I CCNGG 1 cut(s) 547
Bme18I GGWCC 1 cut(s) 718
BmeT110I CYCGRG 1 cut(s) 236
BmgBI CACGTC 1 cut(s) 670
BmgT120I GGNCC 2 cut(s) 203, 718
BmiI GGNNCC 5 cut(s) 205, 621, 704, 719, 720
BmrFI CCNGG 1 cut(s) 547
BmsI GCATC 2 cut(s) 622, 970
BpiI GAAGAC 2 cut(s) 420, 838
BsaAI YACGTR 1 cut(s) 854
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 3 cut(s) 339, 545, 546
BsaWI WCCGGW 2 cut(s) 520, 705
Bsc4I CCNNNNNNNGG 1 cut(s) 712
BseAI TCCGGA 1 cut(s) 520
BseBI CCWGG 1 cut(s) 547
BseDI CCNNGG 3 cut(s) 339, 545, 546
BseLI CCNNNNNNNGG 1 cut(s) 712
BseMII CTCAG 1 cut(s) 581
BseRI GAGGAG 5 cut(s) 50, 53, 56, 507, 758
BseXI GCAGC 5 cut(s) 274, 280, 286, 308, 590
BsgI GTGCAG 2 cut(s) 62, 365
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 2 cut(s) 521, 706
BslFI GGGAC 3 cut(s) 392, 680, 731
BslI CCNNNNNNNGG 1 cut(s) 712
BsmFI GGGAC 3 cut(s) 392, 680, 731
BsnI GGCC 1 cut(s) 204
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 237
Bsp13I TCCGGA 1 cut(s) 520
Bsp1407I TGTACA 1 cut(s) 651
Bsp143I GATC 4 cut(s) 472, 643, 838, 901
Bsp19I CCATGG 1 cut(s) 339
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 1 cut(s) 580
BspEI TCCGGA 1 cut(s) 520
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 5 cut(s) 205, 621, 704, 719, 720
BspMAI CTGCAG 1 cut(s) 298
BspPI GGATC 3 cut(s) 467, 638, 896
BspQI GCTCTTC 1 cut(s) 225
BsrBI CCGCTC 2 cut(s) 14, 96
BsrGI TGTACA 1 cut(s) 651
BssECI CCNNGG 3 cut(s) 339, 545, 546
BssMI GATC 4 cut(s) 472, 643, 838, 901
BssNI GRCGYC 1 cut(s) 486
BssT1I CCWWGG 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 547
Bst4CI ACNGT 1 cut(s) 913
Bst6I CTCTTC 5 cut(s) 23, 225, 321, 584, 921
BstACI GRCGYC 1 cut(s) 486
BstAUI TGTACA 1 cut(s) 651
BstBAI YACGTR 1 cut(s) 854
BstC8I GCNNGC 3 cut(s) 202, 437, 971
BstDEI CTNAG 2 cut(s) 567, 965
BstDSI CCRYGG 1 cut(s) 339
BstFNI CGCG 1 cut(s) 174
BstHHI GCGC 2 cut(s) 176, 200
BstKTI GATC 4 cut(s) 475, 646, 841, 904
BstMBI GATC 4 cut(s) 472, 643, 838, 901
BstMWI GCNNNNNNNGC 8 cut(s) 293, 296, 299, 302, 305, 308, 311, 314
BstNI CCWGG 1 cut(s) 547
BstSCI CCNGG 1 cut(s) 545
BstSFI CTRYAG 2 cut(s) 294, 697
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 5 cut(s) 274, 280, 286, 308, 590
BstV2I GAAGAC 2 cut(s) 420, 838
BstX2I RGATCY 1 cut(s) 643
BstYI RGATCY 1 cut(s) 643
BsuRI GGCC 1 cut(s) 204
BtgI CCRYGG 1 cut(s) 339
BtgZI GCGATG 1 cut(s) 319
BtrI CACGTC 1 cut(s) 670
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 3 cut(s) 74, 808, 918
Cac8I GCNNGC 3 cut(s) 202, 437, 971
CfoI GCGC 2 cut(s) 176, 200
Cfr13I GGNCC 2 cut(s) 203, 718
Csp6I GTAC 3 cut(s) 274, 574, 652
CviAII CATG 4 cut(s) 220, 340, 836, 952
CviJI RGCY 9 cut(s) 204, 235, 287, 299, 357, 435, 603, 788, 973
CviKI_1 RGCY 9 cut(s) 204, 235, 287, 299, 357, 435, 603, 788, 973
CviQI GTAC 3 cut(s) 274, 574, 652
DdeI CTNAG 2 cut(s) 567, 965
DpnI GATC 4 cut(s) 474, 645, 840, 903
DpnII GATC 4 cut(s) 472, 643, 838, 901
Eam1104I CTCTTC 5 cut(s) 23, 225, 321, 584, 921
EarI CTCTTC 5 cut(s) 23, 225, 321, 584, 921
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 339
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 718
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 481, 856
Eco88I CYCGRG 1 cut(s) 236
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 2 cut(s) 203, 718
EcoRII CCWGG 1 cut(s) 545
EcoT14I CCWWGG 1 cut(s) 339
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 4 cut(s) 223, 343, 839, 955
FaiI YATR 8 cut(s) 221, 341, 492, 510, 537, 753, 837, 953
FalI AAGNNNNNCTT 8 cut(s) 245, 277, 419, 451, 581, 613, 668, 700
FaqI GGGAC 3 cut(s) 392, 680, 731
FatI CATG 4 cut(s) 219, 339, 835, 951
FblI GTMKAC 1 cut(s) 246
FriOI GRGCYC 1 cut(s) 237
FspBI CTAG 3 cut(s) 284, 639, 1003
GlaI GCGC 2 cut(s) 175, 199
HaeIII GGCC 1 cut(s) 204
HapII CCGG 2 cut(s) 521, 706
HhaI GCGC 2 cut(s) 176, 200
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 4 cut(s) 223, 343, 839, 955
Hin6I GCGC 2 cut(s) 174, 198
HinP1I GCGC 2 cut(s) 174, 198
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 3 cut(s) 380, 541, 761
HpaII CCGG 2 cut(s) 521, 706
Hpy166II GTNNAC 4 cut(s) 247, 366, 682, 883
Hpy188I TCNGA 7 cut(s) 385, 405, 461, 500, 570, 661, 875
Hpy188III TCNNGA 3 cut(s) 470, 476, 521
Hpy8I GTNNAC 4 cut(s) 247, 366, 682, 883
Hpy99I CGWCG 5 cut(s) 182, 185, 191, 212, 230
HpyAV CCTTC 5 cut(s) 100, 172, 250, 670, 880
HpyCH4III ACNGT 1 cut(s) 913
HpyCH4IV ACGT 3 cut(s) 486, 669, 853
HpyCH4V TGCA 3 cut(s) 79, 296, 346
HpyF10VI GCNNNNNNNGC 8 cut(s) 293, 296, 299, 302, 305, 308, 311, 314
HpyF3I CTNAG 2 cut(s) 567, 965
HpySE526I ACGT 3 cut(s) 486, 669, 853
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 4 cut(s) 223, 343, 839, 955
HspAI GCGC 2 cut(s) 174, 198
KflI GGGWCCC 1 cut(s) 718
Kpn2I TCCGGA 1 cut(s) 520
Kzo9I GATC 4 cut(s) 472, 643, 838, 901
LguI GCTCTTC 1 cut(s) 225
LmnI GCTCC 3 cut(s) 101, 562, 640
Lsp1109I GCAGC 5 cut(s) 274, 280, 286, 308, 590
LweI GCATC 2 cut(s) 622, 970
MaeI CTAG 3 cut(s) 284, 639, 1003
MaeII ACGT 3 cut(s) 486, 669, 853
MaeIII GTNAC 3 cut(s) 511, 593, 613
MalI GATC 4 cut(s) 474, 645, 840, 903
MbiI CCGCTC 2 cut(s) 14, 96
MboI GATC 4 cut(s) 472, 643, 838, 901
MboII GAAGA 9 cut(s) 10, 242, 338, 425, 445, 601, 828, 843, 938
MfeI CAATTG 1 cut(s) 994
MflI RGATCY 1 cut(s) 643
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 3 cut(s) 419, 756, 994
MlyI GAGTC 2 cut(s) 374, 755
MroI TCCGGA 1 cut(s) 520
MroXI GAANNNNTTC 1 cut(s) 260
MseI TTAA 1 cut(s) 408
MslI CAYNNNNRTG 3 cut(s) 338, 888, 911
MspA1I CMGCKG 2 cut(s) 293, 299
MspI CCGG 2 cut(s) 521, 706
MspR9I CCNGG 1 cut(s) 547
MunI CAATTG 1 cut(s) 994
MvaI CCWGG 1 cut(s) 547
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 8 cut(s) 293, 296, 299, 302, 305, 308, 311, 314
NcoI CCATGG 1 cut(s) 339
NdeII GATC 4 cut(s) 472, 643, 838, 901
NlaIII CATG 4 cut(s) 223, 343, 839, 955
NlaIV GGNNCC 5 cut(s) 205, 621, 704, 719, 720
NmuCI GTSAC 1 cut(s) 593
OliI CACNNNNGTG 2 cut(s) 888, 911
PaeR7I CTCGAG 1 cut(s) 236
PasI CCCWGGG 1 cut(s) 546
PciSI GCTCTTC 1 cut(s) 225
PcsI WCGNNNNNNNCGW 5 cut(s) 174, 189, 222, 234, 243
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 1 cut(s) 541
PleI GAGTC 2 cut(s) 374, 755
PpsI GAGTC 2 cut(s) 374, 755
Ppu21I YACGTR 1 cut(s) 854
PpuMI RGGWCCY 1 cut(s) 718
Psp124BI GAGCTC 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 718
Psp6I CCWGG 1 cut(s) 545
PspGI CCWGG 1 cut(s) 545
PspN4I GGNNCC 5 cut(s) 205, 621, 704, 719, 720
PspPI GGNCC 2 cut(s) 203, 718
PspPPI RGGWCCY 1 cut(s) 718
PspXI VCTCGAGB 1 cut(s) 236
PstI CTGCAG 1 cut(s) 298
PsuI RGATCY 1 cut(s) 643
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 3 cut(s) 275, 575, 653
RsaNI GTAC 3 cut(s) 274, 574, 652
RseI CAYNNNNRTG 3 cut(s) 338, 888, 911
SacI GAGCTC 1 cut(s) 237
SalI GTCGAC 1 cut(s) 245
SapI GCTCTTC 1 cut(s) 225
SaqAI TTAA 1 cut(s) 408
Sau3AI GATC 4 cut(s) 472, 643, 838, 901
Sau96I GGNCC 2 cut(s) 203, 718
SchI GAGTC 2 cut(s) 374, 755
ScrFI CCNGG 1 cut(s) 547
SduI GDGCHC 1 cut(s) 237
SfaNI GCATC 2 cut(s) 622, 970
SfcI CTRYAG 2 cut(s) 294, 697
Sfr274I CTCGAG 1 cut(s) 236
SinI GGWCC 1 cut(s) 718
SlaI CTCGAG 1 cut(s) 236
SmiMI CAYNNNNRTG 3 cut(s) 338, 888, 911
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
Sse9I AATT 3 cut(s) 419, 756, 994
SspMI CTAG 3 cut(s) 284, 639, 1003
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 1 cut(s) 545
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 1 cut(s) 913
TaiI ACGT 3 cut(s) 489, 672, 856
TaqI TCGA 4 cut(s) 237, 246, 264, 580
TasI AATT 3 cut(s) 419, 756, 994
TatI WGTACW 2 cut(s) 573, 651
TauI GCSGC 6 cut(s) 293, 305, 308, 311, 314, 317
TfiI GAWTC 1 cut(s) 541
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 3 cut(s) 81, 808, 918
TseFI GTSAC 1 cut(s) 593
TseI GCWGC 5 cut(s) 287, 293, 296, 299, 603
Tsp45I GTSAC 1 cut(s) 593
TspDTI ATGAA 1 cut(s) 445
TspGWI ACGGA 1 cut(s) 632
TspRI CASTG 3 cut(s) 81, 808, 918
VpaK11BI GGWCC 1 cut(s) 718
XapI RAATTY 1 cut(s) 419
XcmI CCANNNNNNNNNTGG 1 cut(s) 949
XhoI CTCGAG 1 cut(s) 236
XmiI GTMKAC 1 cut(s) 246
XmnI GAANNNNTTC 1 cut(s) 260
XspI CTAG 3 cut(s) 284, 639, 1003
ZraI GACGTC 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.