FvH4_2g28320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011527
Physical Location & Seq
Reverse (-)
22270751 .. 22271452
702 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28320.t1

Sequence Viewer

Length: 702 bp
ATGATGAACGAATACGGAACCATATCGCCGCCGGGAAGCAACGCCAATCTGAGGGAGAAGCAGCGCCACCGATCGATGTGCCTCCCTGTGTGTTTCTCGAGCTCCACGCATCCTTTTGACGTCTTGGACGACGAGGACTACGTCCGGACGCCGCGGTCGCCGATGATGCCGGAGATCAGGGACCGCTGCCGGAATCTCATTTCCAAAATCGGAGGACACAGACGCCGGCGCGCGGCGTCGGCCGACTTCAGCTACGACGCTTACAACTACTCGCTCAATTTCGAGGACGAGACCACACGCGCCGACGATATTATCACCCGGTCGCGGCTGCCGCCCTCCGCCTCCGGGAGCCTGACACCGCCGGAGAAGTCGACCACGAGCCGGAATATTCTGGACGACGTTCTGGAGGAAGATGAGGACAGCCGGGGTTGGAGCTGGAGGAGGTCGGCGCCGGAGAATAATCCGAAGAGCCGGAACAACGTGTCGAGGATTGGACTACGGGGACGGAACCGCCACTCGTCCACGTCGGCCGATTTCAGCTACGAGCCGTCCAGCTACGCGCTCAATTTCGAAGATGACAGCAGCAAAGCGGACGAGCTTGGCCCTGTCCATGGATTCATGGCGCGTTTGCCGCACTCGCCGACGTGTAAGAGCTCGGCAACGCCACCGCCTGCCAAGTGTTCGATTGCGGCCTTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

234

Amino Acids

26.12

Weight (kDa)

7.01

Isoelectric Point (pI)

74.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 154
AatII GACGTC 1 cut(s) 123
AccB1I GGYRCC 1 cut(s) 448
AccI GTMKAC 1 cut(s) 371
AccII CGCG 7 cut(s) 154, 231, 233, 300, 325, 560, 625
AccIII TCCGGA 1 cut(s) 144
AcoI YGGCCR 2 cut(s) 240, 528
AcuI CTGAAG 1 cut(s) 232
AcyI GRCGYC 5 cut(s) 120, 149, 223, 236, 449
AfiI CCNNNNNNNGG 6 cut(s) 51, 232, 324, 345, 381, 611
AflIII ACRYGT 2 cut(s) 480, 644
AjiI CACGTC 2 cut(s) 525, 645
AluBI AGCT 8 cut(s) 102, 252, 435, 540, 555, 598, 654, 699
AluI AGCT 8 cut(s) 102, 252, 435, 540, 555, 598, 654, 699
Alw21I GWGCWC 2 cut(s) 104, 656
Alw26I GTCTC 1 cut(s) 284
Ama87I CYCGRG 1 cut(s) 97
Aor13HI TCCGGA 1 cut(s) 144
AoxI GGCC 4 cut(s) 240, 528, 601, 690
ApeKI GCWGC 4 cut(s) 61, 186, 328, 582
AspLEI GCGC 7 cut(s) 66, 231, 233, 302, 451, 562, 625
AspS9I GGNCC 2 cut(s) 181, 602
AsuC2I CCSGG 4 cut(s) 33, 319, 346, 425
AsuHPI GGTGA 1 cut(s) 307
AsuII TTCGAA 1 cut(s) 570
AvaI CYCGRG 1 cut(s) 97
AvaII GGWCC 1 cut(s) 181
BanI GGYRCC 1 cut(s) 448
BanII GRGCYC 2 cut(s) 104, 656
BauI CACGAG 1 cut(s) 376
Bbv12I GWGCWC 2 cut(s) 104, 656
BbvI GCAGC 4 cut(s) 73, 173, 315, 594
BceAI ACGGC 1 cut(s) 532
BcgI CGANNNNNNTGC 2 cut(s) 60, 94
BcnI CCSGG 4 cut(s) 33, 319, 346, 425
BcoDI GTCTC 1 cut(s) 284
BfoI RGCGCY 2 cut(s) 67, 452
Bme1390I CCNGG 4 cut(s) 33, 319, 346, 425
Bme18I GGWCC 1 cut(s) 181
BmeT110I CYCGRG 1 cut(s) 97
BmgBI CACGTC 2 cut(s) 525, 645
BmgT120I GGNCC 2 cut(s) 181, 602
BmiI GGNNCC 5 cut(s) 19, 182, 350, 450, 509
BmrFI CCNGG 4 cut(s) 33, 319, 346, 425
BmsI GCATC 2 cut(s) 118, 156
BpmI CTGGAG 2 cut(s) 425, 457
Bpu14I TTCGAA 1 cut(s) 570
BpuMI CCSGG 4 cut(s) 33, 319, 346, 425
Bsa29I ATCGAT 1 cut(s) 74
BsaHI GRCGYC 5 cut(s) 120, 149, 223, 236, 449
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 3 cut(s) 152, 424, 610
BsaWI WCCGGW 1 cut(s) 144
Bsc4I CCNNNNNNNGG 6 cut(s) 51, 232, 324, 345, 381, 611
Bse118I RCCGGY 1 cut(s) 225
BseAI TCCGGA 1 cut(s) 144
BseCI ATCGAT 1 cut(s) 74
BseDI CCNNGG 3 cut(s) 152, 424, 610
BseGI GGATG 1 cut(s) 109
BseLI CCNNNNNNNGG 6 cut(s) 51, 232, 324, 345, 381, 611
BseMII CTCAG 1 cut(s) 41
BsePI GCGCGC 1 cut(s) 229
BseRI GAGGAG 1 cut(s) 454
BseX3I CGGCCG 2 cut(s) 240, 528
BseXI GCAGC 4 cut(s) 73, 173, 315, 594
Bsh1236I CGCG 7 cut(s) 154, 231, 233, 300, 325, 560, 625
Bsh1285I CGRYCG 5 cut(s) 74, 158, 243, 323, 531
BshFI GGCC 4 cut(s) 242, 530, 603, 692
BshNI GGYRCC 1 cut(s) 448
BshVI ATCGAT 1 cut(s) 74
BsiEI CGRYCG 5 cut(s) 74, 158, 243, 323, 531
BsiHKAI GWGCWC 2 cut(s) 104, 656
BsiHKCI CYCGRG 1 cut(s) 97
BslFI GGGAC 2 cut(s) 194, 516
BslI CCNNNNNNNGG 6 cut(s) 51, 232, 324, 345, 381, 611
BsmAI GTCTC 1 cut(s) 284
BsmFI GGGAC 2 cut(s) 194, 516
BsnI GGCC 4 cut(s) 242, 530, 603, 692
Bso31I GGTCTC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 97
Bsp119I TTCGAA 1 cut(s) 570
Bsp1286I GDGCHC 2 cut(s) 104, 656
Bsp13I TCCGGA 1 cut(s) 144
Bsp143I GATC 2 cut(s) 71, 174
Bsp19I CCATGG 1 cut(s) 610
BspANI GGCC 4 cut(s) 242, 530, 603, 692
BspCNI CTCAG 1 cut(s) 42
BspDI ATCGAT 1 cut(s) 74
BspEI TCCGGA 1 cut(s) 144
BspFNI CGCG 7 cut(s) 154, 231, 233, 300, 325, 560, 625
BspLI GGNNCC 5 cut(s) 19, 182, 350, 450, 509
BspQI GCTCTTC 1 cut(s) 461
BspT104I TTCGAA 1 cut(s) 570
BspT107I GGYRCC 1 cut(s) 448
BspTNI GGTCTC 1 cut(s) 284
BsrFI RCCGGY 1 cut(s) 225
BssAI RCCGGY 1 cut(s) 225
BssECI CCNNGG 3 cut(s) 152, 424, 610
BssHII GCGCGC 1 cut(s) 229
BssMI GATC 2 cut(s) 71, 174
BssNI GRCGYC 5 cut(s) 120, 149, 223, 236, 449
BssSI CACGAG 1 cut(s) 376
BssT1I CCWWGG 1 cut(s) 610
Bst2BI CACGAG 1 cut(s) 376
Bst6I CTCTTC 1 cut(s) 461
BstACI GRCGYC 5 cut(s) 120, 149, 223, 236, 449
BstBI TTCGAA 1 cut(s) 570
BstC8I GCNNGC 3 cut(s) 227, 231, 672
BstDEI CTNAG 1 cut(s) 50
BstDSI CCRYGG 2 cut(s) 152, 610
BstF5I GGATG 1 cut(s) 109
BstFNI CGCG 7 cut(s) 154, 231, 233, 300, 325, 560, 625
BstH2I RGCGCY 2 cut(s) 67, 452
BstHHI GCGC 7 cut(s) 66, 231, 233, 302, 451, 562, 625
BstKTI GATC 2 cut(s) 74, 177
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 2 cut(s) 71, 174
BstMCI CGRYCG 5 cut(s) 74, 158, 243, 323, 531
BstMWI GCNNNNNNNGC 6 cut(s) 157, 166, 239, 331, 631, 637
BstSCI CCNGG 4 cut(s) 31, 317, 344, 423
BstUI CGCG 7 cut(s) 154, 231, 233, 300, 325, 560, 625
BstV1I GCAGC 4 cut(s) 73, 173, 315, 594
BstZI CGGCCG 2 cut(s) 240, 528
Bsu15I ATCGAT 1 cut(s) 74
BsuRI GGCC 4 cut(s) 242, 530, 603, 692
BsuTUI ATCGAT 1 cut(s) 74
BtgI CCRYGG 2 cut(s) 152, 610
BtrI CACGTC 2 cut(s) 525, 645
BtsCI GGATG 1 cut(s) 109
Cac8I GCNNGC 3 cut(s) 227, 231, 672
CfoI GCGC 7 cut(s) 66, 231, 233, 302, 451, 562, 625
Cfr10I RCCGGY 1 cut(s) 225
Cfr13I GGNCC 2 cut(s) 181, 602
Cfr42I CCGCGG 1 cut(s) 155
ClaI ATCGAT 1 cut(s) 74
CseI GACGC 4 cut(s) 157, 225, 231, 266
CviAII CATG 2 cut(s) 611, 619
DdeI CTNAG 1 cut(s) 50
DinI GGCGCC 1 cut(s) 450
DpnI GATC 2 cut(s) 73, 176
DpnII GATC 2 cut(s) 71, 174
DrdI GACNNNNNNGTC 1 cut(s) 154
DseDI GACNNNNNNGTC 1 cut(s) 154
EaeI YGGCCR 2 cut(s) 240, 528
EagI CGGCCG 2 cut(s) 240, 528
Eam1104I CTCTTC 1 cut(s) 461
EarI CTCTTC 1 cut(s) 461
EciI GGCGGA 1 cut(s) 328
Ecl136II GAGCTC 2 cut(s) 102, 654
EclXI CGGCCG 2 cut(s) 240, 528
Eco130I CCWWGG 1 cut(s) 610
Eco24I GRGCYC 2 cut(s) 104, 656
Eco31I GGTCTC 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 181
Eco52I CGGCCG 2 cut(s) 240, 528
Eco53kI GAGCTC 2 cut(s) 102, 654
Eco57I CTGAAG 1 cut(s) 232
Eco88I CYCGRG 1 cut(s) 97
EcoICRI GAGCTC 2 cut(s) 102, 654
EcoT14I CCWWGG 1 cut(s) 610
EcoT38I GRGCYC 2 cut(s) 104, 656
EgeI GGCGCC 1 cut(s) 450
EheI GGCGCC 1 cut(s) 450
ErhI CCWWGG 1 cut(s) 610
FaeI CATG 2 cut(s) 614, 622
FaiI YATR 3 cut(s) 23, 612, 620
FaqI GGGAC 2 cut(s) 194, 516
FatI CATG 2 cut(s) 610, 618
FblI GTMKAC 1 cut(s) 371
FokI GGATG 1 cut(s) 96
FriOI GRGCYC 2 cut(s) 104, 656
GlaI GCGC 7 cut(s) 65, 230, 232, 301, 450, 561, 624
GsuI CTGGAG 2 cut(s) 425, 457
HaeII RGCGCY 2 cut(s) 67, 452
HaeIII GGCC 4 cut(s) 242, 530, 603, 692
HgaI GACGC 4 cut(s) 157, 225, 231, 266
HhaI GCGC 7 cut(s) 66, 231, 233, 302, 451, 562, 625
Hin1I GRCGYC 5 cut(s) 120, 149, 223, 236, 449
Hin1II CATG 2 cut(s) 614, 622
Hin6I GCGC 7 cut(s) 64, 229, 231, 300, 449, 560, 623
HinP1I GCGC 7 cut(s) 64, 229, 231, 300, 449, 560, 623
HincII GTYRAC 1 cut(s) 372
HindII GTYRAC 1 cut(s) 372
HinfI GANTC 2 cut(s) 193, 615
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 2 cut(s) 372, 522
Hpy188I TCNGA 3 cut(s) 51, 212, 465
Hpy188III TCNNGA 4 cut(s) 97, 145, 392, 404
Hpy8I GTNNAC 2 cut(s) 372, 522
Hpy99I CGWCG 7 cut(s) 134, 241, 260, 308, 401, 529, 646
HpyCH4IV ACGT 6 cut(s) 120, 141, 399, 480, 524, 644
HpyF10VI GCNNNNNNNGC 6 cut(s) 157, 166, 239, 331, 631, 637
HpyF3I CTNAG 1 cut(s) 50
HpySE526I ACGT 6 cut(s) 120, 141, 399, 480, 524, 644
Hsp92I GRCGYC 5 cut(s) 120, 149, 223, 236, 449
Hsp92II CATG 2 cut(s) 614, 622
HspAI GCGC 7 cut(s) 64, 229, 231, 300, 449, 560, 623
KasI GGCGCC 1 cut(s) 448
Kpn2I TCCGGA 1 cut(s) 144
KroI GCCGGC 1 cut(s) 225
KroNI GCCGGC 1 cut(s) 227
KspI CCGCGG 1 cut(s) 155
Kzo9I GATC 2 cut(s) 71, 174
LguI GCTCTTC 1 cut(s) 461
LmnI GCTCC 3 cut(s) 107, 348, 432
Lsp1109I GCAGC 4 cut(s) 73, 173, 315, 594
LweI GCATC 2 cut(s) 118, 156
MaeII ACGT 6 cut(s) 120, 141, 399, 480, 524, 644
MalI GATC 2 cut(s) 73, 176
MboI GATC 2 cut(s) 71, 174
MboII GAAGA 3 cut(s) 422, 478, 584
MhlI GDGCHC 2 cut(s) 104, 656
MluCI AATT 2 cut(s) 277, 565
Mly113I GGCGCC 1 cut(s) 449
MmeI TCCRAC 1 cut(s) 410
MreI CGCCGGCG 1 cut(s) 225
MroI TCCGGA 1 cut(s) 144
MroNI GCCGGC 1 cut(s) 225
MspA1I CMGCKG 2 cut(s) 154, 186
MspR9I CCNGG 4 cut(s) 33, 319, 346, 425
MvnI CGCG 7 cut(s) 154, 231, 233, 300, 325, 560, 625
MwoI GCNNNNNNNGC 6 cut(s) 157, 166, 239, 331, 631, 637
NaeI GCCGGC 1 cut(s) 227
NarI GGCGCC 1 cut(s) 449
NciI CCSGG 4 cut(s) 33, 319, 346, 425
NcoI CCATGG 1 cut(s) 610
NdeII GATC 2 cut(s) 71, 174
NgoMIV GCCGGC 1 cut(s) 225
NlaIII CATG 2 cut(s) 614, 622
NlaIV GGNNCC 5 cut(s) 19, 182, 350, 450, 509
NmeAIII GCCGAG 1 cut(s) 635
NspV TTCGAA 1 cut(s) 570
PaeR7I CTCGAG 1 cut(s) 97
PauI GCGCGC 1 cut(s) 229
PciSI GCTCTTC 1 cut(s) 461
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 138
PdiI GCCGGC 1 cut(s) 227
PfeI GAWTC 2 cut(s) 193, 615
PflFI GACNNNGTC 1 cut(s) 140
PfoI TCCNGGA 1 cut(s) 344
Ple19I CGATCG 1 cut(s) 74
PluTI GGCGCC 1 cut(s) 452
Psp124BI GAGCTC 2 cut(s) 104, 656
PspN4I GGNNCC 5 cut(s) 19, 182, 350, 450, 509
PspPI GGNCC 2 cut(s) 181, 602
PsyI GACNNNGTC 1 cut(s) 140
PteI GCGCGC 1 cut(s) 229
PvuI CGATCG 1 cut(s) 74
SacI GAGCTC 2 cut(s) 104, 656
SacII CCGCGG 1 cut(s) 155
SalI GTCGAC 1 cut(s) 370
SapI GCTCTTC 1 cut(s) 461
Sau3AI GATC 2 cut(s) 71, 174
Sau96I GGNCC 2 cut(s) 181, 602
ScrFI CCNGG 4 cut(s) 33, 319, 346, 425
SduI GDGCHC 2 cut(s) 104, 656
SfaNI GCATC 2 cut(s) 118, 156
SfoI GGCGCC 1 cut(s) 450
Sfr274I CTCGAG 1 cut(s) 97
Sfr303I CCGCGG 1 cut(s) 155
SfuI TTCGAA 1 cut(s) 570
SgrAI CRCCGGYG 1 cut(s) 225
SgrBI CCGCGG 1 cut(s) 155
SinI GGWCC 1 cut(s) 181
SlaI CTCGAG 1 cut(s) 97
SmlI CTYRAG 1 cut(s) 97
SmoI CTYRAG 1 cut(s) 97
Sse9I AATT 2 cut(s) 277, 565
SspDI GGCGCC 1 cut(s) 448
SspI AATATT 1 cut(s) 388
SstI GAGCTC 2 cut(s) 104, 656
StyD4I CCNGG 4 cut(s) 31, 317, 344, 423
StyI CCWWGG 1 cut(s) 610
TaiI ACGT 6 cut(s) 123, 144, 402, 483, 527, 647
TaqI TCGA 7 cut(s) 74, 98, 282, 371, 485, 570, 683
TasI AATT 2 cut(s) 277, 565
TauI GCSGC 7 cut(s) 31, 154, 236, 328, 334, 634, 692
TfiI GAWTC 2 cut(s) 193, 615
TseI GCWGC 4 cut(s) 61, 186, 328, 582
TspDTI ATGAA 2 cut(s) 20, 607
TspGWI ACGGA 2 cut(s) 30, 520
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 181
XhoI CTCGAG 1 cut(s) 97
XmiI GTMKAC 1 cut(s) 371
ZraI GACGTC 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.