FvH4_2g28350

PHD-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004222
Physical Location & Seq
Forward (+)
22282684 .. 22299015
16332 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28350.t1

Sequence Viewer

Length: 6459 bp
ATGGAGAACCGGTGGATGAACAAATGCAACTCGACATTGCCGCCTGCGGCTTCGTCGTCGTCTTCTCAGGAGCAACGGAATCAGATGGAGAATGCAGGCTATTTGTCTTGTCAGCGTGGCTCACAAGATTTGAGACCGGCGATGCTGGGTTGGTTGCAGGATCCTCTGCTTTCCAGCATCCAGAGTTCAGGTTCCCACAGATCAGAACATGTTAATTTGGGAAATTCTTTTCTGTCTCTCCTATCTGGGTCACCATCGCTATTGCAGCGTGGTTTTCAAGATTTCTCAAATTCCCAACCTATCTGTACCTCTGGTAAAATTTTACCAGTGGGTAATAATTCCATTCTAAACAGTACTCAAAGTACCATTCCATTGTCATCCACCGGGCTACCATCAGAAAAATTGAGTTGGACAAATCTGCAAAGTGGAACAGACTTCTGCCACAATGGTTCATCAAAGGTAGTGCCAAGTTCCATTTGTGCCAGCAATTCTGTATTGCATGATCTGCAGAGTTCAGACCTGGCCAAGGTAGTCATCTGTCACACGGGCCCTGTTAATGAGAAGCTAGAGAGTTCTTATGCCTTGAGTAGGGAATGGCACTGTGCTGGTCCTGCATCTAGAGCAAATATTCAAAATTCCGAAAGAATGCCACTGGAAGCAAATTCCTTCATATCTAATCAGGCATATAGAGTATGTCATAGTGCTAGTCCTGCAGATTCTTGGAAGGCTCGTAGAGAAAGCATTCATACTTCACAAACAATACCACTGGAAGCAAACTCTTTTATTTCTTATCATGCATCTAGTCTATGGCATGGCACCAATCCTGCAGATAATGGGAAGGCTTGTAGAGCTAACATTGAAACTTCACCAAAAATGCCCCAAGCATCCAGTTTTATGAATGGTTGCCCTCGTGTTTTCTGCTCGACTACTAGTGGCTATCTACTTTTTAGCAATACAGGATTTCTTGGTATTGTTTGCTCTTGCCATTCTTTTCGCATGTCTGCCTTTAAGTTTTGTGAGCATTCAGGATTATATGGTGTTAACCCGGGGGATGCCATTCGCATGGACAGTGGAGAGACCATTTCTCAATGGTGCAAGCTCTATCTCCCGAAGTTTGGGATTAGGATCCCTGGAGATAAGAGTGAATGGGACTGGCCTGAAGAATTATCAGCAACTGCCAGTTTGATGAAACGTAGTGTGCCTATGCCAAAGATATCTAATAGCTCGTCTGATTTGGTCTTCACACGTGGAGGTTCAGTAAGCTCTAAACAGTCTTTTGATGGTGTTCCGCTATCAAAGAATCTTATCACATGTCAGAGTTTGGTTATTAGTGCCGTATCAAATAAACCTGAAGGGAACAGTCAGGATAGTAACAACCCATTTCTCAAGGCTTTGACTGGTACTTCACAGAGTAATTTGCAAATGGCAGATAACATGACAATGGAGCGTGCCATGGCAACTTCAAAGCTAGTTGGAAATGGAGCAGAAGATAGTTGCCAGTTTATTTCTTCTTATACTGGATCTGTGCCCAATAGAACTTCCATTGCCCACCCACCTTTACAGGAACGAAGAATCAATGGTAAAGAATCAGACTTTCGCAGAATTGAAAACACAAGGGATGGTGCCTTCAGAGATGCTGCAATTTCAAATATTGAGCTTAGGCTTGGTCAACCGTACCAATTGGCTCAAACTTCAGGAAATACTGACCTGTCAGCTGTAGGGCCGCCACTCTTGGGCACGGTTGTAAATCCAATGAAATCACTCTTCCCTCAACAGATGAATGCTAGCAGAGCGAATTGCAGGGAAGAAGTGGAATTTATGCAATGTGATCGTCTTTCTGCCAACCCGTCCAATCCCAGCAGGAATAGAAATTGGAACCAGTTGAACCATGGCAATAATGCATTTGTAATCAGGAATGGCACAGATGATGAAAGAGCACAGAATTCTGTAATTTCACTTCTTACCAATCTGAAATCTCCATGTAAAGAAAATAAACCATCAAAAGCCAATAACTCTATGTTCAATGTCAGTGGGAATTCGATGAGAAATACACTACATTCTGAACCTCTATCTGACAAGAACGACCTCGCTACTGTCTGGCGGAGCGGAGGTAATAGTGAAAGGCAACTGGACATGTCGCACTTGGGTTCCTATAAACTAAATGACAATGATAAAGGGCTGAGTTCTGCTGCTCATGCCTCTCAACTTGCAAAGGATTTGGGGTTCAGAATACGCAAGGAGATGGAAGTGTCTAGTTCTTTCAATAGGTTGAGTGGTAATGGGGATCCTAATTTCTCAACAGCACATAGAAACAGCTGCTACTCCCATCAGTTGTCTGGTGTGCCACTGGGTACACCTGAATCCAAAATAATGTCCAATTATCCTGAGAAGGTTAATTCTTTGGCAAATAGTGGGCAAGTTGATCATGTCTATCTTCGACCCATGGCTTCATCTATGGGATCTGGGATTCCTACTCAGGCAGTCTCAAAAGGTATTCCTGTGAGTGCATCAACCTCTTTAGCAGATCTGATACCGCCTTTCTATAGGGAAGAGTTTGTTGGAGTTCACACTCATTTGCCTGATGATACATTACAAGTGCATGCAACAAGGCAAATGCAAGAGATATCGAAGTTGCCTTCCCCTTCCAAGAACCAAGGGGAAGGAAGAGTTGGTTGCTCTACTTATATGCAGCAGTCTCGCGTAGATACTTCAGCATCTGGGAAGCAAAGCCACAAACTTAGTCTTAGCGATAAACATGATGTTTCTGAAGCTGGGGTTAATCCACATCCATCTGATGTTACCTGTAGGATTGGAACTGATGAAGGTTTTGCTTCCCTGACAGGTGTTAACTGCTGTTGTCAATTCTCACAATATAAACAAGGAAATGCAATACATTTTAAAGAAGTTGGTCTGAAACATCAAACTTCAGTTGTTCCTCTTTGTAAGGAGCAGCCCTCACCAAGAAGTGAAAAGTCAAAGAATGTCCCTGAGCCTAGTGAGCATGAAAGGTGCTGCCACAAAGTGCCCTGTGGTAATTTTCGGGGCAGCTCCAGTCATGCAGCCTACAGAAATTGTTTAGAAATGAATTCTGAATCAAGAGTTGGAAGCTTTTCTGCGGTGTCTAAGGTACAAATGGGAACAGTCAACAGTGAAGCCTCTATGATACTTTCTCCACAATTTTCTAACAGCCATTTAATTCCGAAGGATAAAACTGTTTCCTTGGATCATAAGAGAAAGCTGAGTGGGGAAGTAACCAAGAACAATGCTTATCATACTTCTCAGTGGAGAGATGTGCCAAGTAAGGTCAAAGGGGTTTCTGATGTGACGCGTGTGGACCGCTTAGCTAATCTGTTTGATGCAACAAGAGAAGACAGAGAGAAACTTGGAGATACTTGTGTTAAATGCTTCAATGGGACTGTGCAGATAGCGGACTCCATGAAAGAGCATGAAGTGTCTAATATATCTTCTGGATGCTCTGCTCCTGTGGTTAGTCAGCCATCAATAGAGTTTAACAATATGGAATCTTCAACTAATGATCCTGGGGATCATGGATGTGGAAGCAATTTTGTTGTTGATGAAGGATCAGGCATTGACAAAGCGTGGTCATCAGATGATGCACTTGAAAGTGAAAGAAGTGCCAAGTTCCTTGCCTCTACTGGTTCTAGCCTTAAAAAAGTTGGGGCTCCCAAGAATTTAAATCATGAATCTTCATCTTGCCTTCTTGATGACCTAAAACTACTAAACTCTTTAACATGGCAGAAAGGTCGAGATCAAATCCCAGCTGGGCTTGCCCTTCGTGATAAAGACAAACATCTTCAGAACTTAGAACAAGGCTTGAAGATTGGGAAGAGAAAGAGAGAACTAGCATTGGAATTGAATGCTTCATGTTCCAATTCAGATTCTTCTCGAGTACGACAAGAGAATCACAATAGTAATGGTACTTCACAGTTCACCTCCCAACCATCAAAGAGTTTAATGATGCTTTCAACATCAAGGAAATCGGGAACTCATGTCACTGGAAATTGCATTACTCAATCTAGTTCTAAACCCAGACTACATATATCATCTTCAGCAAAGAAACTCTTGCTTAGAAGTGATTTACACAAACTGCATGATGACAAAGAGAGTGAGGTGAATAATGTTTTCCAGACAGAATTAAATGGTGGTGCTAACAATCATGAACTTCCTGAAGTTTCTGGTGGGAAGACATGTAAAAGAGATTGCAGTTCCAATGCTTTCAGGCAGTTCCAGATACAAGAATCTAGTAGAAAAGATACTAAAAGAACCAAGTACAATTCTGTAGATGGTTTCAAATCGACATGTAGTCAGCAAGTGAAGATTGGTCATAGGAAAGCAAGGCCAATAGTATGCGGAATATATGGTGAATTAACTGATGGAAGTTCGACTGGACGAATGTCAAAACCGGCAAAACTTGTTCCTCTCAGCAGGGTTCTTAATTCTTCCCGCAAGTGCATACTCCCCAAACTTTGCAACTCTAAATCAAGTTCCATGAGGAAGAAGAAACTTGGTGGGGCTGCCATATGCAATACATATGACTTGAAGACTGAAAAATACAAATGTCATGATGCTATGGTTAAAGTTAATGATACTTCCATGAGAAAAAAGAAGAAAGAATGCTCCCCTGGAGAGAGAGAGATTCATAAAGAGCTGTTCTCAATGGAGAAACAAGGAGATGTTCAAAGTGAAAAGGATCATCAAAAGCTAGACAGTATTACCCATACTCAATTGCAGATGAAGCCTAAGGAAATTCGGAAACGCAGCATATATGAATTTACAGAAAAAGGGGACGATACTGGCTTTAAAAGTTCTTCTGTATCAAAGATTTCAAATTTCCGGCCAGCAAACGACGGCAAACTTGTAAATACTGGTGAAGATAGTGGGTTATGCCAACATAGTGCTAAAAATTCTACTCAAGAGCACAGGTGCCATTGTAATTGCGACTCAGATCCAATTTGCTGCGTCTGTGGTAGCTCAAACCAAGATGAAATCAATATTTTATTGGAGTGTAGTCAATGTTCAGTCAGAGTGCATCAGGCTTGTTATGGTGTGTCCAAAGTACCTAAGGGCTGTTGGTCTTGCAGACCATGTCGGATGAGCTCAAAAGATATTGTATGCGTTCTTTGTGGTTATGGCGGTGGTGCCATGACTCAAGCACTACGGAGTCAGACAATTGCTGTAAGCATTTTAAGAGCTTGGAACATTGAAACGGAATGTGGGCCTAAGAATGAGCTATGTTCTATTAAAACTTTGCAAAAAGACTCAACTGGATTGCATTGCTCGGGCTATAGACATTCTGAAAGCTCTTCCCTTTTTGTGTCTCAACAAAGTGGCCAACCTTTGGCAGCAGCTCATTGTAAAAGGGGTATGTCATACAGAGTGGATGGTGTTGAGAATTCCCCTAGTGTTAGTAAGACAAAGGTACATAACAGCATTACCATGGGATTAGTTGATTCGGCTACTAAACAGTGGGTTCATATGGTTTGCGGGCTTTGGACTCCTGAAACACGATGCCCCAATGTTGACACCATGAGTGCTTTTGATGTCTCTTGTGTTCCCCTCTCCACCGATGATGCGGTTTGCTGTATGTGTAAGCGAGCGGGGGGCTCATGTATACAGTGCAGGGTTGAAAATTGTTCTGTTCGATTTCACCCATGGTGTGCTCATCAGAAGGGTCTGTTGCAAACTGAGGTTGAAGGTGTTGACAATGAGAATGTTGGCTTTTATGGAAGATGTGGACTACATGCTACACATCCCATATACAAATCAGAATACCCTGTTGACACTGAAGCTGGTTGTCTGGACGAAAAGAAATTAGTGTGCGCCAGAACTGAGGGTTACAAGGGCCGTAAACGTGATGGCTTCAGGCATAACTATTGTGATCGATCGAAGGGAAGTGATGGATGCCTTGTTCCTCAGGAGCAGTTGAATGCTTGGGCGTATATTAATGGGCAGAAGTCATGCACACAGGAGCTTCCTAAACTGGCAATATCTGAAATCGAGCATGACTCTCGGAAAGAATACACACGCTATAAGCAAGCAAAGTTGTGGAAGCATCTTGTAGTATACAAATCTGGCATACATGCCCTTGGTCTTTACACATCTCGGTTCATTTCCCGTGATGAAATGGTTGTCGAGTATGTTGGAGAAATTGTGGGGCAACGTGTTTCTGATAAGAGGGAAAATGAATATCAATCTGCAAAAAAACTTCAGTACAAGAGCGCGTGCTACTTTTTCAGGATAGACAAAGAGCATATCATTGATGCTACCTGCAAAGGAGGGATTGCTCGATTTGTGAACCATTCATGCTCGCCAAATTGCGTTGCCAAGGTGATATCAGTCAGGAATGAAAAAAAGGTTGTATTTCTTGCTGAGAGGGATATTTTCCCGGGAGAAGAGATAACATATGACTACCATTTTAACCATGAGGACGAAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD_2 SET zf-HC5HC2H_2

Protein Analysis

2153

Amino Acids

236.57

Weight (kDa)

8.69

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 322 - 366 5.2e-13 Tify domain binding domain
PHD_2 PF13831 1671 - 1703 1e-08 PHD-finger
zf-HC5HC2H_2 PF13832 1826 - 1908 5.5e-17 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 1832 - 1921 1.9e-12 PHD-like zinc-binding domain
SET PF00856 2036 - 2144 3.9e-24 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 6296
AccB1I GGYRCC 4 cut(s) 815, 1622, 4944, 5159
AccB7I CCANNNNNTGG 1 cut(s) 5359
AccBSI CCGCTC 2 cut(s) 2106, 5618
AccI GTMKAC 2 cut(s) 5632, 6084
AccII CGCG 3 cut(s) 2700, 3328, 6242
AcoI YGGCCR 3 cut(s) 522, 4856, 5350
AcvI CACGTG 1 cut(s) 1247
AdeI CACNNNGTG 1 cut(s) 3022
AfiI CCNNNNNNNGG 5 cut(s) 526, 588, 1115, 1733, 5359
AflIII ACRYGT 8 cut(s) 208, 1244, 1310, 2133, 3326, 4208, 4319, 6181
AgeI ACCGGT 1 cut(s) 9
AhdI GACNNNNNGTC 1 cut(s) 4323
AhlI ACTAGT 1 cut(s) 929
AjnI CCWGG 4 cut(s) 519, 1129, 3538, 4642
AjuI GAANNNNNNNTTGG 6 cut(s) 2541, 2573, 2652, 2684, 3084, 3116
AloI GAACNNNNNNTCC 4 cut(s) 2207, 2239, 5913, 5945
Alw21I GWGCWC 4 cut(s) 1939, 4941, 5120, 5683
Alw26I GTCTC 7 cut(s) 127, 240, 1070, 2488, 2700, 5343, 5569
AlwNI CAGNNNCTG 2 cut(s) 1175, 2717
Ama87I CYCGRG 4 cut(s) 1045, 3906, 5299, 6406
AoxI GGCC 9 cut(s) 522, 547, 1154, 1721, 4358, 4856, 5237, 5350, 5863
ApaI GGGCCC 1 cut(s) 551
AseI ATTAAT 1 cut(s) 5964
AsiGI ACCGGT 1 cut(s) 9
Asp700I GAANNNNTTC 2 cut(s) 741, 3109
AspLEI GCGC 2 cut(s) 5843, 6242
AspS9I GGNCC 7 cut(s) 547, 548, 608, 1721, 3334, 5237, 5863
AsuC2I CCSGG 5 cut(s) 385, 1046, 1047, 6407, 6408
AsuHPI GGTGA 9 cut(s) 243, 858, 2949, 3943, 4144, 4394, 4901, 5660, 6361
AsuNHI GCTAGC 1 cut(s) 1784
AvaI CYCGRG 4 cut(s) 1045, 3906, 5299, 6406
AvaII GGWCC 2 cut(s) 608, 3334
AxyI CCTNAGG 3 cut(s) 4761, 5082, 5934
BaeGI GKGCMC 4 cut(s) 551, 1530, 1739, 3027
BaeI ACNNNNGTAYC 2 cut(s) 3381, 3414
BalI TGGCCA 2 cut(s) 524, 5352
BamHI GGATCC 3 cut(s) 160, 1125, 2284
BanI GGYRCC 4 cut(s) 815, 1622, 4944, 5159
BanII GRGCYC 4 cut(s) 551, 3685, 5120, 5627
BarI GAAGNNNNNNTAC 4 cut(s) 1941, 1973, 3925, 3957
BauI CACGAG 1 cut(s) 909
BbrPI CACGTG 1 cut(s) 1247
BbsI GAAGAC 5 cut(s) 54, 1231, 3375, 4211, 4568
Bbv12I GWGCWC 4 cut(s) 1939, 4941, 5120, 5683
BceAI ACGGC 3 cut(s) 1319, 4885, 5850
BcgI CGANNNNNNTGC 8 cut(s) 1811, 1845, 2678, 2712, 3746, 3780, 6011, 6045
BciT130I CCWGG 4 cut(s) 521, 1131, 3540, 4644
BclI TGATCA 1 cut(s) 2422
BcnI CCSGG 5 cut(s) 385, 1046, 1047, 6407, 6408
BcoDI GTCTC 7 cut(s) 127, 240, 1070, 2488, 2700, 5343, 5569
BcuI ACTAGT 1 cut(s) 929
BfmI CTRYAG 9 cut(s) 506, 711, 825, 1716, 2542, 2803, 3064, 4299, 5305
BfuAI ACCTGC 1 cut(s) 6296
BglII AGATCT 1 cut(s) 2524
BlpI GCTNAGC 1 cut(s) 3340
BmcAI AGTACT 1 cut(s) 355
Bme1390I CCNGG 9 cut(s) 385, 521, 1046, 1047, 1131, 3540, 4644, 6407, 6408
Bme18I GGWCC 2 cut(s) 608, 3334
BmeRI GACNNNNNGTC 1 cut(s) 4323
BmeT110I CYCGRG 4 cut(s) 1045, 3906, 5299, 6406
BmgT120I GGNCC 7 cut(s) 547, 548, 608, 1721, 3334, 5237, 5863
BmrFI CCNGG 9 cut(s) 385, 521, 1046, 1047, 1131, 3540, 4644, 6407, 6408
BmrI ACTGGG 1 cut(s) 2357
BmtI GCTAGC 1 cut(s) 1788
BmuI ACTGGG 1 cut(s) 2357
BoxI GACNNNNGTC 1 cut(s) 4414
BpiI GAAGAC 5 cut(s) 54, 1231, 3375, 4211, 4568
BplI GAGNNNNNCTC 6 cut(s) 2939, 2971, 4658, 4690, 6011, 6043
BpmI CTGGAG 3 cut(s) 1152, 3034, 4665
Bpu10I CCTNAGC 2 cut(s) 1658, 2988
Bpu1102I GCTNAGC 1 cut(s) 3340
BpuEI CTTGAG 4 cut(s) 604, 1370, 4917, 5154
BpuMI CCSGG 5 cut(s) 385, 1046, 1047, 6407, 6408
Bsa29I ATCGAT 1 cut(s) 5902
BsaAI YACGTR 1 cut(s) 1247
BsaBI GATNNNNATC 1 cut(s) 1124
BsaI GGTCTC 2 cut(s) 127, 1070
BsaWI WCCGGW 1 cut(s) 9
BsaXI ACNNNNNCTCC 7 cut(s) 26, 1242, 1272, 2095, 2125, 6156, 6186
Bsc4I CCNNNNNNNGG 5 cut(s) 526, 588, 1115, 1733, 5359
Bse118I RCCGGY 3 cut(s) 9, 136, 4423
Bse21I CCTNAGG 3 cut(s) 4761, 5082, 5934
Bse3DI GCAATG 4 cut(s) 35, 1542, 1829, 5293
Bse8I GATNNNNATC 1 cut(s) 1124
BseBI CCWGG 4 cut(s) 521, 1131, 3540, 4644
BseCI ATCGAT 1 cut(s) 5902
BseJI GATNNNNATC 1 cut(s) 1124
BseLI CCNNNNNNNGG 5 cut(s) 526, 588, 1115, 1733, 5359
BseMI GCAATG 4 cut(s) 35, 1542, 1829, 5293
BseSI GKGCMC 4 cut(s) 551, 1530, 1739, 3027
BseYI CCCAGC 5 cut(s) 145, 1856, 2771, 3778, 3782
BsgI GTGCAG 2 cut(s) 3440, 5659
Bsh1236I CGCG 3 cut(s) 2700, 3328, 6242
Bsh1285I CGRYCG 1 cut(s) 5906
BshFI GGCC 9 cut(s) 524, 549, 1156, 1723, 4360, 4858, 5239, 5352, 5865
BshNI GGYRCC 4 cut(s) 815, 1622, 4944, 5159
BshTI ACCGGT 1 cut(s) 9
BshVI ATCGAT 1 cut(s) 5902
BsiEI CGRYCG 1 cut(s) 5906
BsiHKAI GWGCWC 4 cut(s) 1939, 4941, 5120, 5683
BsiHKCI CYCGRG 4 cut(s) 1045, 3906, 5299, 6406
BsiSI CCGG 7 cut(s) 10, 137, 384, 1046, 4424, 4855, 6407
BslFI GGGAC 4 cut(s) 1163, 2969, 3427, 4820
BslI CCNNNNNNNGG 5 cut(s) 526, 588, 1115, 1733, 5359
BsmAI GTCTC 7 cut(s) 127, 240, 1070, 2488, 2700, 5343, 5569
BsmFI GGGAC 4 cut(s) 1163, 2969, 3427, 4820
BsmI GAATGC 8 cut(s) 97, 651, 741, 1021, 1786, 3883, 4640, 5953
BsnI GGCC 9 cut(s) 524, 549, 1156, 1723, 4360, 4858, 5239, 5352, 5865
Bso31I GGTCTC 2 cut(s) 127, 1070
BsoBI CYCGRG 4 cut(s) 1045, 3906, 5299, 6406
Bsp120I GGGCCC 1 cut(s) 547
Bsp1720I GCTNAGC 1 cut(s) 3340
Bsp19I CCATGG 5 cut(s) 1452, 1888, 2442, 5457, 5672
BspANI GGCC 9 cut(s) 524, 549, 1156, 1723, 4360, 4858, 5239, 5352, 5865
BspDI ATCGAT 1 cut(s) 5902
BspFNI CGCG 3 cut(s) 2700, 3328, 6242
BspHI TCATGA 3 cut(s) 3700, 4177, 4582
BspMAI CTGCAG 3 cut(s) 510, 715, 829
BspMI ACCTGC 1 cut(s) 6296
BspOI GCTAGC 1 cut(s) 1788
BspQI GCTCTTC 1 cut(s) 5329
BspT107I GGYRCC 4 cut(s) 815, 1622, 4944, 5159
BspTNI GGTCTC 2 cut(s) 127, 1070
BsrBI CCGCTC 2 cut(s) 2106, 5618
BsrDI GCAATG 4 cut(s) 35, 1542, 1829, 5293
BsrFI RCCGGY 3 cut(s) 9, 136, 4423
BssAI RCCGGY 3 cut(s) 9, 136, 4423
BssNAI GTATAC 2 cut(s) 5633, 6085
BssSI CACGAG 1 cut(s) 909
Bst1107I GTATAC 2 cut(s) 5633, 6085
Bst2BI CACGAG 1 cut(s) 909
Bst2UI CCWGG 4 cut(s) 521, 1131, 3540, 4644
Bst6I CTCTTC 6 cut(s) 1769, 2544, 2659, 3842, 5329, 6408
BstAPI GCANNNNNTGC 1 cut(s) 505
BstBAI YACGTR 1 cut(s) 1247
BstDSI CCRYGG 5 cut(s) 1452, 1888, 2442, 5457, 5672
BstEII GGTNACC 1 cut(s) 249
BstENI CCTNNNNNAGG 2 cut(s) 524, 586
BstFNI CGCG 3 cut(s) 2700, 3328, 6242
BstHHI GCGC 2 cut(s) 5843, 6242
BstMAI GTCTC 7 cut(s) 127, 240, 1070, 2488, 2700, 5343, 5569
BstMCI CGRYCG 1 cut(s) 5906
BstNI CCWGG 4 cut(s) 521, 1131, 3540, 4644
BstNSI RCATGY 9 cut(s) 212, 1000, 1314, 2137, 2603, 4212, 4323, 5765, 6104
BstPAI GACNNNNGTC 1 cut(s) 4414
BstPI GGTNACC 1 cut(s) 249
BstSCI CCNGG 9 cut(s) 383, 519, 1044, 1045, 1129, 3538, 4642, 6405, 6406
BstSFI CTRYAG 9 cut(s) 506, 711, 825, 1716, 2542, 2803, 3064, 4299, 5305
BstSLI GKGCMC 4 cut(s) 551, 1530, 1739, 3027
BstUI CGCG 3 cut(s) 2700, 3328, 6242
BstV2I GAAGAC 5 cut(s) 54, 1231, 3375, 4211, 4568
BstX2I RGATCY 7 cut(s) 160, 1125, 1520, 2284, 2459, 2524, 4966
BstXI CCANNNNNNTGG 1 cut(s) 1063
BstYI RGATCY 7 cut(s) 160, 1125, 1520, 2284, 2459, 2524, 4966
BstZ17I GTATAC 2 cut(s) 5633, 6085
Bsu15I ATCGAT 1 cut(s) 5902
Bsu36I CCTNAGG 3 cut(s) 4761, 5082, 5934
BsuRI GGCC 9 cut(s) 524, 549, 1156, 1723, 4360, 4858, 5239, 5352, 5865
BsuTUI ATCGAT 1 cut(s) 5902
BtgI CCRYGG 5 cut(s) 1452, 1888, 2442, 5457, 5672
BtgZI GCGATG 2 cut(s) 155, 240
BveI ACCTGC 1 cut(s) 6296
CaiI CAGNNNCTG 2 cut(s) 1175, 2717
CciI TCATGA 3 cut(s) 3700, 4177, 4582
CfoI GCGC 2 cut(s) 5843, 6242
Cfr10I RCCGGY 3 cut(s) 9, 136, 4423
Cfr13I GGNCC 7 cut(s) 547, 548, 608, 1721, 3334, 5237, 5863
Cfr9I CCCGGG 2 cut(s) 1045, 6406
ClaI ATCGAT 1 cut(s) 5902
CseI GACGC 2 cut(s) 3334, 4969
CspAI ACCGGT 1 cut(s) 9
CspCI CAANNNNNGTGG 2 cut(s) 2011, 2046
DraI TTTAAA 3 cut(s) 2899, 3696, 4822
DraIII CACNNNGTG 1 cut(s) 3022
DriI GACNNNNNGTC 1 cut(s) 4323
EaeI YGGCCR 3 cut(s) 522, 4856, 5350
Eam1104I CTCTTC 6 cut(s) 1769, 2544, 2659, 3842, 5329, 6408
Eam1105I GACNNNNNGTC 1 cut(s) 4323
EarI CTCTTC 6 cut(s) 1769, 2544, 2659, 3842, 5329, 6408
EciI GGCGGA 1 cut(s) 2116
Ecl136II GAGCTC 1 cut(s) 5118
Eco24I GRGCYC 4 cut(s) 551, 3685, 5120, 5627
Eco31I GGTCTC 2 cut(s) 127, 1070
Eco32I GATATC 3 cut(s) 1215, 2625, 6354
Eco47I GGWCC 2 cut(s) 608, 3334
Eco53kI GAGCTC 1 cut(s) 5118
Eco72I CACGTG 1 cut(s) 1247
Eco81I CCTNAGG 3 cut(s) 4761, 5082, 5934
Eco88I CYCGRG 4 cut(s) 1045, 3906, 5299, 6406
Eco91I GGTNACC 1 cut(s) 249
EcoICRI GAGCTC 1 cut(s) 5118
EcoNI CCTNNNNNAGG 2 cut(s) 524, 586
EcoO109I RGGNCCY 1 cut(s) 548
EcoO65I GGTNACC 1 cut(s) 249
EcoRI GAATTC 4 cut(s) 1942, 2035, 3085, 5413
EcoRII CCWGG 4 cut(s) 519, 1129, 3538, 4642
EcoRV GATATC 3 cut(s) 1215, 2625, 6354
EcoT22I ATGCAT 2 cut(s) 799, 1903
EcoT38I GRGCYC 4 cut(s) 551, 3685, 5120, 5627
FalI AAGNNNNNCTT 2 cut(s) 4067, 4099
FaqI GGGAC 4 cut(s) 1163, 2969, 3427, 4820
FauI CCCGC 3 cut(s) 4472, 5498, 5611
FauNDI CATATG 4 cut(s) 4541, 4552, 5496, 6424
FbaI TGATCA 1 cut(s) 2422
FblI GTMKAC 2 cut(s) 5632, 6084
FriOI GRGCYC 4 cut(s) 551, 3685, 5120, 5627
GlaI GCGC 2 cut(s) 5842, 6241
GsaI CCCAGC 5 cut(s) 149, 1860, 2775, 3782, 3786
GsuI CTGGAG 3 cut(s) 1152, 3034, 4665
HaeIII GGCC 9 cut(s) 524, 549, 1156, 1723, 4360, 4858, 5239, 5352, 5865
HapII CCGG 7 cut(s) 10, 137, 384, 1046, 4424, 4855, 6407
HgaI GACGC 2 cut(s) 3334, 4969
HhaI GCGC 2 cut(s) 5843, 6242
Hin6I GCGC 2 cut(s) 5841, 6240
HinP1I GCGC 2 cut(s) 5841, 6240
HincII GTYRAC 7 cut(s) 1042, 1670, 2848, 3145, 5542, 5722, 5800
HindII GTYRAC 7 cut(s) 1042, 1670, 2848, 3145, 5542, 5722, 5800
HindIII AAGCTT 1 cut(s) 3106
HpaI GTTAAC 2 cut(s) 1042, 2848
HpaII CCGG 7 cut(s) 10, 137, 384, 1046, 4424, 4855, 6407
HphI GGTGA 9 cut(s) 243, 858, 2949, 3943, 4144, 4394, 4901, 5660, 6361
Hpy99I CGWCG 3 cut(s) 58, 61, 4871
HpyCH4IV ACGT 4 cut(s) 1192, 1246, 5872, 6181
HpySE526I ACGT 4 cut(s) 1192, 1246, 5872, 6181
HspAI GCGC 2 cut(s) 5841, 6240
Ksp22I TGATCA 1 cut(s) 2422
KspAI GTTAAC 2 cut(s) 1042, 2848
LguI GCTCTTC 1 cut(s) 5329
MaeII ACGT 4 cut(s) 1192, 1246, 5872, 6181
MaeIII GTNAC 8 cut(s) 249, 539, 1370, 2797, 3250, 3322, 4012, 5855
MbiI CCGCTC 2 cut(s) 2106, 5618
MfeI CAATTG 3 cut(s) 1679, 4745, 5190
MflI RGATCY 7 cut(s) 160, 1125, 1520, 2284, 2459, 2524, 4966
MlsI TGGCCA 2 cut(s) 524, 5352
MluI ACGCGT 1 cut(s) 3326
MluNI TGGCCA 2 cut(s) 524, 5352
MlyI GAGTC 7 cut(s) 3425, 4955, 5161, 5191, 5273, 5509, 6020
MmeI TCCRAC 6 cut(s) 389, 1453, 2539, 3082, 5090, 6142
Mox20I TGGCCA 2 cut(s) 524, 5352
Mph1103I ATGCAT 2 cut(s) 799, 1903
MroXI GAANNNNTTC 2 cut(s) 741, 3109
MscI TGGCCA 2 cut(s) 524, 5352
MslI CAYNNNNRTG 4 cut(s) 1061, 1439, 3552, 5456
Msp20I TGGCCA 2 cut(s) 524, 5352
MspA1I CMGCKG 3 cut(s) 1715, 2316, 3782
MspI CCGG 7 cut(s) 10, 137, 384, 1046, 4424, 4855, 6407
MspR9I CCNGG 9 cut(s) 385, 521, 1046, 1047, 1131, 3540, 4644, 6407, 6408
MunI CAATTG 3 cut(s) 1679, 4745, 5190
Mva1269I GAATGC 8 cut(s) 97, 651, 741, 1021, 1786, 3883, 4640, 5953
MvaI CCWGG 4 cut(s) 521, 1131, 3540, 4644
MvnI CGCG 3 cut(s) 2700, 3328, 6242
NciI CCSGG 5 cut(s) 385, 1046, 1047, 6407, 6408
NcoI CCATGG 5 cut(s) 1452, 1888, 2442, 5457, 5672
NdeI CATATG 4 cut(s) 4541, 4552, 5496, 6424
NheI GCTAGC 1 cut(s) 1784
NmuCI GTSAC 4 cut(s) 249, 539, 3322, 4012
NsiI ATGCAT 2 cut(s) 799, 1903
NspI RCATGY 9 cut(s) 212, 1000, 1314, 2137, 2603, 4212, 4323, 5765, 6104
PaeI GCATGC 1 cut(s) 2603
PaeR7I CTCGAG 1 cut(s) 3906
PagI TCATGA 3 cut(s) 3700, 4177, 4582
PciI ACATGT 5 cut(s) 208, 1310, 2133, 4208, 4319
PciSI GCTCTTC 1 cut(s) 5329
PctI GAATGC 8 cut(s) 97, 651, 741, 1021, 1786, 3883, 4640, 5953
PdmI GAANNNNTTC 2 cut(s) 741, 3109
PflFI GACNNNGTC 1 cut(s) 5106
PflMI CCANNNNNTGG 1 cut(s) 5359
PinAI ACCGGT 1 cut(s) 9
Ple19I CGATCG 1 cut(s) 5906
PleI GAGTC 7 cut(s) 3425, 4955, 5161, 5190, 5273, 5509, 6020
PmaCI CACGTG 1 cut(s) 1247
PmlI CACGTG 1 cut(s) 1247
PpsI GAGTC 7 cut(s) 3425, 4955, 5161, 5190, 5273, 5509, 6020
Ppu21I YACGTR 1 cut(s) 1247
PscI ACATGT 5 cut(s) 208, 1310, 2133, 4208, 4319
PshAI GACNNNNGTC 1 cut(s) 4414
PshBI ATTAAT 1 cut(s) 5964
Psp124BI GAGCTC 1 cut(s) 5120
Psp6I CCWGG 4 cut(s) 519, 1129, 3538, 4642
PspCI CACGTG 1 cut(s) 1247
PspEI GGTNACC 1 cut(s) 249
PspFI CCCAGC 5 cut(s) 145, 1856, 2771, 3778, 3782
PspGI CCWGG 4 cut(s) 519, 1129, 3538, 4642
PspOMI GGGCCC 1 cut(s) 547
PspPI GGNCC 7 cut(s) 547, 548, 608, 1721, 3334, 5237, 5863
PsrI GAACNNNNNNTAC 2 cut(s) 5020, 5052
PstI CTGCAG 3 cut(s) 510, 715, 829
PstNI CAGNNNCTG 2 cut(s) 1175, 2717
PsuI RGATCY 7 cut(s) 160, 1125, 1520, 2284, 2459, 2524, 4966
PsyI GACNNNGTC 1 cut(s) 5106
PvuI CGATCG 1 cut(s) 5906
PvuII CAGCTG 3 cut(s) 1715, 2316, 3782
RseI CAYNNNNRTG 4 cut(s) 1061, 1439, 3552, 5456
SacI GAGCTC 1 cut(s) 5120
SapI GCTCTTC 1 cut(s) 5329
Sau96I GGNCC 7 cut(s) 547, 548, 608, 1721, 3334, 5237, 5863
ScaI AGTACT 1 cut(s) 355
SchI GAGTC 7 cut(s) 3425, 4955, 5161, 5191, 5273, 5509, 6020
ScrFI CCNGG 9 cut(s) 385, 521, 1046, 1047, 1131, 3540, 4644, 6407, 6408
SfcI CTRYAG 9 cut(s) 506, 711, 825, 1716, 2542, 2803, 3064, 4299, 5305
Sfr274I CTCGAG 1 cut(s) 3906
SinI GGWCC 2 cut(s) 608, 3334
SlaI CTCGAG 1 cut(s) 3906
SmaI CCCGGG 2 cut(s) 1047, 6408
SmiI ATTTAAAT 1 cut(s) 3696
SmiMI CAYNNNNRTG 4 cut(s) 1061, 1439, 3552, 5456
SmlI CTYRAG 5 cut(s) 583, 1385, 3906, 4932, 5169
SmoI CTYRAG 5 cut(s) 583, 1385, 3906, 4932, 5169
SpeI ACTAGT 1 cut(s) 929
SphI GCATGC 1 cut(s) 2603
SspI AATATT 3 cut(s) 628, 1651, 5014
SstI GAGCTC 1 cut(s) 5120
StyD4I CCNGG 9 cut(s) 383, 519, 1044, 1045, 1129, 3538, 4642, 6405, 6406
SwaI ATTTAAAT 1 cut(s) 3696
TaiI ACGT 4 cut(s) 1195, 1249, 5875, 6184
TatI WGTACW 3 cut(s) 353, 4290, 6231
TauI GCSGC 3 cut(s) 43, 50, 1726
TseFI GTSAC 4 cut(s) 249, 539, 3322, 4012
Tsp45I GTSAC 4 cut(s) 249, 539, 3322, 4012
TspGWI ACGGA 3 cut(s) 91, 5194, 5243
TspMI CCCGGG 2 cut(s) 1045, 6406
Tth111I GACNNNGTC 1 cut(s) 5106
Van91I CCANNNNNTGG 1 cut(s) 5359
VpaK11BI GGWCC 2 cut(s) 608, 3334
VspI ATTAAT 1 cut(s) 5964
XagI CCTNNNNNAGG 2 cut(s) 524, 586
XbaI TCTAGA 1 cut(s) 617
XceI RCATGY 9 cut(s) 212, 1000, 1314, 2137, 2603, 4212, 4323, 5765, 6104
XcmI CCANNNNNNNNNTGG 2 cut(s) 1886, 2333
XhoI CTCGAG 1 cut(s) 3906
XmaI CCCGGG 2 cut(s) 1045, 6406
XmiI GTMKAC 2 cut(s) 5632, 6084
XmnI GAANNNNTTC 2 cut(s) 741, 3109
ZrmI AGTACT 1 cut(s) 355
Zsp2I ATGCAT 2 cut(s) 799, 1903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.