MD15G1130200.v1.1

PHD-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004222
Physical Location & Seq
Forward (+)
9412620 .. 9423786
11167 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1130200.v1.1.491

Sequence Viewer

Length: 6231 bp
ATGGAGAGCTCGTGGAAGCCTAAATATAGTTCGACATTCCCGTCAATGGTTTCCTCGTCCTCTCAGGAGCTTCGGAATCAGATGGAGAGCAATGCAGGTTATTATTCTTATCCACATGGCTCACAAGATTTAAGACCCACATTGCTTGGTAGATTACAAGATCCTGTACTTTCGAGCATTCCGTATTCAGATTCCCAAAGATCAGAACATGCTTCGTTGGGAAATTCATTTCTAGCTCTCTTATCTGGGTCACAATCGGTATTCCAGTGTGATTTTCAAGGATTATCAAATCCAAAGCCTATGGGTACCTCTTGTAAGCGTCTGCCAGATAGTAATAATTTCATTGTGAATGGTACTGGAAGTGCCATTCCAGTCACATCCAGTGGGCTACTGCCAGAAAATATGAGTGGGCAACACCTGCAAGGCGGAGCAGGCTCTTTTCATAAGGTTTCATCGAAGTCAGTCCCAAGCTCCAGTTTTGGAAGCAAATCTGTTTTGTATGATCTGCAGAGTTCGGACCTGGCCAAGGTAGTCACACGTGACATGGTCTCTGGTAGTGAGAAAGTAAAGGGCTCTTTTTCCTTGAGCGGGGAATGGCATGGTGTTAGCGCTGCAGATACTAGGAAGGCAGCGAAGGCTTGTGGAGCAAATATTCAATCTTCAAAAACAAAGTCAATGGAAGCAAGCACCTCTATATCTAACAAAGCATTGGGTTTTATGAATGGTTGCCCTCGTGTTTTTTGTTCAACCACTAGTGGTTATCTACTTCTCAGCAATACAGGACTTGTTGGAATTGTTTGTTCATGCCATTGTCTCCACATGTCTGTCCTGAAATTTTGCGAGCATTCAGGGTTATATGATGTCAACCCGGGGGACATCGTTTGTATGGACAGTGGAGAGACCATTGCCCAGTGGTGCAAGCTCTACTTCCTGAATTCTGGGATTAGGGTTCCGGGAGATCGGAGTGAATGGGACTGGCCTGAAGGATTATCAGCAACTGCCTCTTTGGCGAAATCTAGTGTGACTATGCCCAACATATCCAATGACTTGTCTCATATGGTTCGCTCATCTGGAGGTTCAACAAATTCTCAGCAGTCTTTAGATGGTGCTGCGCTCTTAAAGAGCCTCCTTACAAACCAGAATTCTGCTGTTGGTTCTGTACAAAATAAACAACAAAGGAACATTCAGGATAGCAACAACATTTTTCTCAAGGGTTTGACTGGCACTTCACAGAACAATTTACATGGTATGGCTGATAACCTGATATTGGAGTGCACTGAGTCAATCTCAAATTTGGTTGGTTGTGGACTGCAAGATAGCTGTCAGTCTGCATCTGCTTACGTTGAGTCCATGAAGAATGGAAATTCATCCATTGCCTACCCCACCGTGAAGAATGGAAATTCATCCATTACACACCCATCTTTACAGAACCGAAGAATCATTGGTACAGAATCAAACGTTTGCAGAACTGTAAATGGGAAGGATGGTGCCTTCAGGGATGTTGCAATTTCAAATATTGAGCTGAGGCTTGGTCAACCATACCAGTTGGGTCAAACTCAAACGTCAGGAAACTCAAATTTATCAGCTGTGTGCCCACAGCTATTGGACACACATGCAAATCCACTGAAGTCTCTCTTTCCTGAACAGATGATTCCTAACACAGCCAACTGCAGTGAAGGGGTGAAATTTGGCCAATCTCTTCAGTTTTCTGCTGTTCCATATAATCCCAGCACAAAAAGAGATGGAAAGCAGTTAAACCGTGGAAATAATGCATTCGTAATCAGTAATGCGATAGATGCTGCGAGAGTAGAGAAAACCAATTACAACCTGGTCCAGGATTCTGTGATTTCATTTGTTACCAATCTCAATGCTCCACCTGAGGAAAATTCACGACCAAAAGCCAGTAATTATATATATAATGGAGGTGAGCATGCTATGCGAAGTACACAACATTATGAAACTCATTCTGCCAAGTCTGACCTCACTAGTGGCCCCAGGAATGGAGGTAATAATGTGCAAAGGCAATTGAACATGTCTCAGTTGGGTTCCTATAGACTAATTGACAAGGCTAAAGGGGCAAGTTTTGTCGCTAATGAGTCTCACCTTTCAAAGGATTCAGGGTTTAGAATTCACAAGGAAATTGAAATCTCAAACTCTTCCAATGGATTGAGTGGAAGCAGTGATCCTCGTTTCTTAACAGTACATAAAGACAGCTGCTACTCACACCAGTTGTCTGGTGTAGCACCCAATGGACCTGATTCTAGAAGATGTTCTAATTTTCCTGAGAAGGTTTGTTCTTTTAGTAATAGTGGGCAAGTTGGTCATGTTGATCTCCCACCCTTGACTTCATCGGTAGGATCTGGCTTAGTATTTTCATCAGAGGGAGTCTCAAAATGCATTCCTGTTAGTGCTTCAACATCTGTATCAGATCAGAGATATCATCCACCTGCTTTATCCAGAAAACAGTTAACTGGGGTAACCACTCACTTGCCCAATGATAATTCACGACTGCTTGCACTAAGGCAAATACTGGAGTTATCGAAGCAACACCAGGCATGGCCTTCCCTTCCCATGAACCGAGGGGAAGGAAGATTTGGTTGCTCTTCTTGTATGCAGAATTCTCTTGTTGACACTTCAGCATCCGAGAAGCAAGTCCAACAACTTAGTCATACCAGTAACCATGATGTCACCATTAAATCACACCAACCAGGTGCTAGCTGTAGAATTGGAACTGATGAAGGTTTTACTTCCATGACTGGTGTTAACACCTGCTGTCATTTGTCAACATTGAAACAAGGAACTGCATTACATTCTAAAGAAGTTGATATGAATCATCAAATTTCCTTTGTTCCTCTGTTTAATGAGCAACACTCATTGAGAAGTGGGAAGAACACCAATGAGCCTAGAGGGCATGAAAGATGTTACCACAAAGTTCCCTATGGTCATTTTCATGGCAGCTGCAATTGTGCAGCCAACATAAACTGTTTAGGAATGAATTTTGAAAGAGTTGGGGGTTTTCCTAATGCCTTTAACGAACAAATTGGAACAGTCAATGATGAATCCCCCACGACATTTACTCATAAATGTGCTAACAACCATATTGTTCCAAAGGATAAAACTGTTTCCTTAGATCATAGGGGAAAGTTGAATGGGAAATTACCCAAGAACATCTGTAATGCTTCACAGTGGAAAGATGTTCCGAGTAAGGTCAAAGGAGTTTCTGATGTGATGCGCGTGGACCGTTTATCTAATTTGTTTGATGTGAGAAGACGAGATAATGAGCAGCTTGCAGATACTAGTGTTAAACACTCCAATGGTACTTTGCAGATGGTGGAATCTTCGAAAGAGCATGAAATTTCTAATATTTCTTCTGGATGTTCTGCGCCGGCTGTTACCCAGGCATCAATTGAGGTTAACAAGATAGATTCTTCAACAGTTGATGGTGGGGATAATGGATGTGTAAGTAATCTTGTTTTTGACGAAGGGTCAGGCATTGATAAATGTGGGTCATCAGATGATGCACTTGAAAGTGTAAAAAGTGTGGAGTTCCTTGCCTCAACAAGCTTGAGAAAACTTGGGTCTTTTAAGAATTTAAATCATCAATCATCCGGTAGCCTTCTGGATGAGCTTAAGCTCCTAAACTCTTTAACATGGAGAAAAGGTCAACATAAAATCCCAGCTGGGCTTGACAAATATCCCCAGAACTCTGAGAGAGACTTGGAAACTGGGAAGAAAAAGAGAGAGATGGGTTCCAAGACATGTCCCACTTCAGGTCCTTATCCTGTACAAGAAGAAACCCCCAAATGTAATGACCCTGTTCAGTTTCCCTCTCATCCGTCAAAAAGTGTGAAGATGCTCATTCCATTGGGGCAATCCAAAACTTATACTTTTGGGGCCTGTGCAACTCAGTCCAGTTCCAAACCCAGACTTCGTATATCGTCTTCAGAAAAGAAGCTTTCACGTAAAAGAGATCTGCGCAAACTCTATGATGACAATGACAGAGAGGAAACTGATCTTTACCAGACAGAACCAAGTGGTGGTGCCAACTGTGAAACTGCCGAAGTTTCTGGGGGAAAGAAATGTAAGAGGGATTTCAATTCTAATGGTTCGAGGCATGTCCAAGTACAAGAATCAGGTGAAGAAGGCGCTAGAAAAAGAAAGCAAACATCTGTAGGTTGTTTGAAATCATGTTCTATTAAGCTGGTGAATACTTGTTATAGGAAGGCAAGACCAATAGTATGCGGAAAATATGGTGAACTAGCTAATGGAAGTTTGCCTGGAGACACGCCAAAACCAGCAAAAATTGTTCCCCTCAGCAAGGTTCTTAATTCTTCTAGAAGATGCAAGGTCCCGAAAAATTGTAATCCTAAATCACTTAAGAAAACAATCCCTGGTGGGGCTGTTGTTTGTGGTAAACTAAATGGTACTTCCATGGAAAAAACGAAGAAAGAGTGCTCCGTTGGAGAAAAGAAATGTTGCAATGAGTTGTCCTCATTGGAGAAATTGGGAGAAAATCGACGTGAGAAAGATCATAGTAAGCTAGGCGTTATTGCCCATGCTCAATTGAAGCTGAAGTCTAAGGAAATTCGCAAGCGTAGCATTTATGAATTAACCGAGAAAGGGAAGGACCCCAGCTTTGAAAGTGCTTCTGTTTCGAAGATTTCAAATTGTCTGCCAGCAAAGAGAGAAGGACAACTCCTAAAGAGTGGTGAAAATAAGCTTGGACTCTGCAAACTTAGTGCTAAAACTTCCACTCTAGAGCAACGGTGCCATTCTGATTTGGATTCAGATGCATTCTGCTGTGTTTGTGGTAGCTCAAACAATGATGAAATCAATTATTTACTGGAGTGTAGTCAATGTTCAATCAAGGTGCATCAGGCTTGTTATGGTGTCTCAAAACAACCTAAAGGCCATTGGTATTGCAGGCCATGCCGGACCAGTTCAAAAAATATTGTTTGTGTTCTGTGTGGTTATGGTGGTGGTGCCATGACTCAAGCGCTGCGAAGTCACTCAGTTGTGAAAAGCCTTTTGAAAGCTTGGAACACTGAAACAGAAAGCATGCCTAAGAATAAGTTTTCGTCGGTTAAAACTTTGCAAAAGGATTCAAGTGGGTGGCATTGCTTGACCTCAGCTGTCTGTTATATGGGTATATCCTACCAAGTTGATGTCGTACATAATAGCGTTACAGCGGGATTACTTGATTCGGCAACTAAGCAATGGGTTCATATGGTTTGTGGGCTTTGGACTCCTGGAACACGATGCCCAAATGTTGACACCATGAGTGCTTTTGATGTCTCTGGTGCACATCCTAGAGCAGATGTGGTTTGTTGTATATGTATGCGAGCAGGCGGTTCATGTATACAGTGCAGGATTGCAAATTGTTCTGCTCAGTTTCACCCTTGGTGTGCTCATCAGAAGGGTCTGTTGCAAAGTGAAGCTGAGGGTGTTGACAATGAAAATATTGGTTTTTATGGAAAATGTGTGCTACATGCTACACACCCAATGTGCGAATCAGGCCATGTCCCTGAAACTGGTTGCATTGAAAAACAGGAATTAACTTGCGCTAGAACTGAGGGTTACAAGGGCCGTAAACGTGATGGGTTCAGGCATAATAATTATGGTCAGTCAAAGGGCAATGGTGGATGCCTTGTTCCTCAGGAGCAGTTAAATGCTTGGGTTCATATTAATGGTCAGAAGTCATCCACTCAGGGGCTTCCAAAGCTGCCAGCCTCGGATTTAGAGCATGATTGTCGGAAAGAATATGCACGCTACAAGCAAGCAAAGTTTTGGAAGCATCTAGTGGTGTACAAGTCTGGCATACATGCCCTTGGCCTTTACACGTCTCGGTTCATTTCCCGCGGTGAAATGGTTGTTGAGTATATTGGAGAGATAGTGGGGCTGCGTGTTGCCGACAAAAGGGAAAATGAATACCAATCTGGTAAAAAGCTTCAGTACAAGAGCGCTTGCTACTTCTTCAAGATAGATAAAGAGCATATTATTGATGCCACATGCAAAGGAGGGATTGCTCGTTTTGTCAACCATTCCTGCTCGCCAAACTGCGTCGCCAAAGTGATGTCTGTGAGGAATGAAAAGAAGGTGGTATTTTTCGCAGAGCGGGACATATTCCCCGGAGAAGAGATTACGTATGACTACCACTTCAACCATGAGGATGAAGGTAAGAAGATTCCATGTTTTTGCAATTCAAAGAATTGCAGGCGCTACTTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD_2 SET zf-HC5HC2H_2

Protein Analysis

2077

Amino Acids

226.22

Weight (kDa)

8.89

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 261 - 307 1.7e-13 Tify domain binding domain
PHD_2 PF13831 1612 - 1644 1.5e-09 PHD-finger
zf-HC5HC2H_2 PF13832 1735 - 1811 4.5e-15 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 1742 - 1830 6.2e-11 PHD-like zinc-binding domain
SET PF00856 1943 - 2051 3.6e-25 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 426, 2455, 2777
AasI GACNNNNNNGTC 1 cut(s) 40
Acc16I TGCGCA 1 cut(s) 3977
Acc36I ACCTGC 4 cut(s) 86, 426, 2455, 2777
Acc65I GGTACC 1 cut(s) 305
AccB1I GGYRCC 5 cut(s) 305, 1487, 4040, 4767, 4982
AccB7I CCANNNNNTGG 1 cut(s) 4037
AccBSI CCGCTC 2 cut(s) 588, 6115
AccI GTMKAC 1 cut(s) 5359
AccII CGCG 2 cut(s) 3234, 5859
AciI CCGC 8 cut(s) 426, 588, 4242, 5189, 5349, 5857, 5859, 6115
AclI AACGTT 1 cut(s) 1458
AclWI GGATC 3 cut(s) 155, 2177, 2365
AcoI YGGCCR 2 cut(s) 522, 1690
AcuI CTGAAG 9 cut(s) 1002, 1477, 1646, 1685, 2619, 3753, 3927, 4592, 5933
AcvI CACGTG 1 cut(s) 539
AfeI AGCGCT 3 cut(s) 610, 4998, 5962
AflII CTTAAG 2 cut(s) 3629, 4376
AflIII ACRYGT 5 cut(s) 536, 819, 2031, 3758, 5838
AhdI GACNNNNNGTC 1 cut(s) 3484
AhlI ACTAGT 3 cut(s) 752, 1985, 3296
AjiI CACGTC 2 cut(s) 4520, 5841
AjuI GAANNNNNNNTTGG 4 cut(s) 2577, 2609, 4704, 4736
AloI GAACNNNNNNTCC 2 cut(s) 5634, 5666
Alw21I GWGCWC 5 cut(s) 11, 1277, 4457, 5305, 5410
Alw44I GTGCAC 2 cut(s) 1273, 5301
AlwI GGATC 3 cut(s) 155, 2177, 2365
AlwNI CAGNNNCTG 1 cut(s) 998
Ama87I CYCGRG 1 cut(s) 868
Aor51HI AGCGCT 3 cut(s) 610, 4998, 5962
ApaLI GTGCAC 2 cut(s) 1273, 5301
ArsI GACNNNNNNTTYG 4 cut(s) 656, 688, 4619, 4651
AseI ATTAAT 1 cut(s) 5685
Asp700I GAANNNNTTC 1 cut(s) 2269
Asp718I GGTACC 1 cut(s) 305
AsuC2I CCSGG 4 cut(s) 869, 870, 954, 6129
AsuII TTCGAA 2 cut(s) 3341, 4655
AsuNHI GCTAGC 1 cut(s) 2714
AvaI CYCGRG 1 cut(s) 868
AvaII GGWCC 8 cut(s) 517, 1831, 2252, 3238, 3773, 4348, 4627, 4935
AxyI CCTNAGG 2 cut(s) 1878, 5655
BaeGI GKGCMC 3 cut(s) 1277, 1595, 5305
BaeI ACNNNNGTAYC 4 cut(s) 2406, 2439, 3285, 3318
BalI TGGCCA 2 cut(s) 524, 1692
BanI GGYRCC 5 cut(s) 305, 1487, 4040, 4767, 4982
BanII GRGCYC 2 cut(s) 11, 575
BarI GAAGNNNNNNTAC 2 cut(s) 3925, 3957
BauI CACGAG 2 cut(s) 10, 732
BbrPI CACGTG 1 cut(s) 539
BbsI GAAGAC 2 cut(s) 3274, 3933
Bbv12I GWGCWC 5 cut(s) 11, 1277, 4457, 5305, 5410
BbvCI CCTCAGC 4 cut(s) 1523, 4313, 5128, 5439
BccI CCATC 9 cut(s) 76, 1097, 1426, 1478, 1736, 3322, 3434, 3739, 5591
BceAI ACGGC 1 cut(s) 5571
BcgI CGANNNNNNTGC 2 cut(s) 5732, 5766
BcnI CCSGG 4 cut(s) 869, 870, 954, 6129
BcuI ACTAGT 3 cut(s) 752, 1985, 3296
BfmI CTRYAG 6 cut(s) 506, 612, 1669, 2050, 2719, 4170
BfoI RGCGCY 5 cut(s) 612, 4149, 5000, 5964, 6220
BfrI CTTAAG 2 cut(s) 3629, 4376
BfuAI ACCTGC 4 cut(s) 86, 426, 2455, 2777
BglI GCCNNNNNGGC 2 cut(s) 1007, 2908
BglII AGATCT 1 cut(s) 3970
Bme18I GGWCC 8 cut(s) 517, 1831, 2252, 3238, 3773, 4348, 4627, 4935
BmeRI GACNNNNNGTC 1 cut(s) 3484
BmeT110I CYCGRG 1 cut(s) 868
BmgBI CACGTC 2 cut(s) 4520, 5841
BmrI ACTGGG 3 cut(s) 904, 2481, 3735
BmtI GCTAGC 1 cut(s) 2718
BmuI ACTGGG 3 cut(s) 904, 2481, 3735
BpiI GAAGAC 2 cut(s) 3274, 3933
BplI GAGNNNNNCTC 8 cut(s) 1271, 1303, 2372, 2404, 2855, 2887, 4475, 4507
BpmI CTGGAG 5 cut(s) 457, 1092, 2552, 4299, 4865
Bpu10I CCTNAGC 4 cut(s) 1523, 4313, 5128, 5439
Bpu14I TTCGAA 2 cut(s) 3341, 4655
BpuEI CTTGAG 4 cut(s) 604, 1193, 3586, 4977
BpuMI CCSGG 4 cut(s) 869, 870, 954, 6129
BsaAI YACGTR 3 cut(s) 539, 3962, 6144
BsaBI GATNNNNATC 1 cut(s) 2829
BsaI GGTCTC 2 cut(s) 553, 893
BsaWI WCCGGW 1 cut(s) 3608
BsaXI ACNNNNNCTCC 5 cut(s) 26, 3646, 3676, 5877, 5907
Bse118I RCCGGY 1 cut(s) 3385
Bse21I CCTNAGG 2 cut(s) 1878, 5655
Bse3DI GCAATG 8 cut(s) 97, 140, 903, 1371, 4486, 5116, 5222, 5641
Bse8I GATNNNNATC 1 cut(s) 2829
BseJI GATNNNNATC 1 cut(s) 2829
BseMI GCAATG 8 cut(s) 97, 140, 903, 1371, 4486, 5116, 5222, 5641
BseSI GKGCMC 3 cut(s) 1277, 1595, 5305
BseYI CCCAGC 4 cut(s) 1727, 3676, 3680, 4631
BsgI GTGCAG 2 cut(s) 2988, 5386
Bsh1236I CGCG 2 cut(s) 3234, 5859
BshNI GGYRCC 5 cut(s) 305, 1487, 4040, 4767, 4982
BsiHKAI GWGCWC 5 cut(s) 11, 1277, 4457, 5305, 5410
BsiHKCI CYCGRG 1 cut(s) 868
BsiSI CCGG 6 cut(s) 869, 953, 3386, 3609, 4933, 6129
BslFI GGGAC 7 cut(s) 449, 887, 986, 3747, 4334, 5507, 6131
BsmBI CGTCTC 1 cut(s) 5847
BsmFI GGGAC 7 cut(s) 449, 887, 986, 3747, 4334, 5507, 6131
BsmI GAATGC 5 cut(s) 177, 844, 1772, 2397, 4793
Bso31I GGTCTC 2 cut(s) 553, 893
BsoBI CYCGRG 1 cut(s) 868
Bsp119I TTCGAA 2 cut(s) 3341, 4655
Bsp1286I GDGCHC 7 cut(s) 11, 575, 1277, 1595, 4457, 5305, 5410
Bsp1407I TGTACA 3 cut(s) 1159, 3784, 5805
Bsp19I CCATGG 1 cut(s) 4431
BspACI CCGC 8 cut(s) 426, 588, 4242, 5189, 5349, 5857, 5859, 6115
BspFNI CGCG 2 cut(s) 3234, 5859
BspMAI CTGCAG 3 cut(s) 510, 616, 1673
BspMI ACCTGC 4 cut(s) 86, 426, 2455, 2777
BspOI GCTAGC 1 cut(s) 2718
BspPI GGATC 3 cut(s) 155, 2177, 2365
BspQI GCTCTTC 1 cut(s) 2608
BspT104I TTCGAA 2 cut(s) 3341, 4655
BspT107I GGYRCC 5 cut(s) 305, 1487, 4040, 4767, 4982
BspTI CTTAAG 2 cut(s) 3629, 4376
BspTNI GGTCTC 2 cut(s) 553, 893
BsrBI CCGCTC 2 cut(s) 588, 6115
BsrDI GCAATG 8 cut(s) 97, 140, 903, 1371, 4486, 5116, 5222, 5641
BsrFI RCCGGY 1 cut(s) 3385
BsrGI TGTACA 3 cut(s) 1159, 3784, 5805
BssAI RCCGGY 1 cut(s) 3385
BssNAI GTATAC 1 cut(s) 5360
BssSI CACGAG 2 cut(s) 10, 732
BssT1I CCWWGG 4 cut(s) 525, 4431, 5399, 5827
Bst1107I GTATAC 1 cut(s) 5360
Bst2BI CACGAG 2 cut(s) 10, 732
Bst6I CTCTTC 4 cut(s) 1704, 2161, 2608, 6129
BstAFI CTTAAG 2 cut(s) 3629, 4376
BstAPI GCANNNNNTGC 3 cut(s) 418, 1936, 4929
BstAUI TGTACA 3 cut(s) 1159, 3784, 5805
BstBAI YACGTR 3 cut(s) 539, 3962, 6144
BstBI TTCGAA 2 cut(s) 3341, 4655
BstDSI CCRYGG 3 cut(s) 1759, 4431, 5857
BstEII GGTNACC 1 cut(s) 2476
BstENI CCTNNNNNAGG 4 cut(s) 524, 1832, 2108, 4316
BstFNI CGCG 2 cut(s) 3234, 5859
BstH2I RGCGCY 5 cut(s) 612, 4149, 5000, 5964, 6220
BstPI GGTNACC 1 cut(s) 2476
BstSFI CTRYAG 6 cut(s) 506, 612, 1669, 2050, 2719, 4170
BstSLI GKGCMC 3 cut(s) 1277, 1595, 5305
BstSNI TACGTA 1 cut(s) 6144
BstUI CGCG 2 cut(s) 3234, 5859
BstV2I GAAGAC 2 cut(s) 3274, 3933
BstX2I RGATCY 3 cut(s) 160, 2357, 3970
BstXI CCANNNNNNTGG 1 cut(s) 2234
BstYI RGATCY 3 cut(s) 160, 2357, 3970
BstZ17I GTATAC 1 cut(s) 5360
Bsu36I CCTNAGG 2 cut(s) 1878, 5655
BtgI CCRYGG 3 cut(s) 1759, 4431, 5857
BtrI CACGTC 2 cut(s) 4520, 5841
BtsI GCAGTG 2 cut(s) 1678, 2185
BveI ACCTGC 4 cut(s) 86, 426, 2455, 2777
CaiI CAGNNNCTG 1 cut(s) 998
Cfr10I RCCGGY 1 cut(s) 3385
Cfr42I CCGCGG 1 cut(s) 5860
Cfr9I CCCGGG 1 cut(s) 868
CseI GACGC 2 cut(s) 308, 6049
CsiI ACCWGGT 2 cut(s) 1827, 2707
CspCI CAANNNNNGTGG 4 cut(s) 127, 162, 1584, 1619
DraI TTTAAA 1 cut(s) 3594
DrdI GACNNNNNNGTC 1 cut(s) 40
DriI GACNNNNNGTC 1 cut(s) 3484
DseDI GACNNNNNNGTC 1 cut(s) 40
EaeI YGGCCR 2 cut(s) 522, 1690
Eam1104I CTCTTC 4 cut(s) 1704, 2161, 2608, 6129
Eam1105I GACNNNNNGTC 1 cut(s) 3484
EarI CTCTTC 4 cut(s) 1704, 2161, 2608, 6129
EciI GGCGGA 1 cut(s) 441
Ecl136II GAGCTC 1 cut(s) 9
Eco105I TACGTA 1 cut(s) 6144
Eco130I CCWWGG 4 cut(s) 525, 4431, 5399, 5827
Eco24I GRGCYC 2 cut(s) 11, 575
Eco31I GGTCTC 2 cut(s) 553, 893
Eco32I GATATC 1 cut(s) 2438
Eco47I GGWCC 8 cut(s) 517, 1831, 2252, 3238, 3773, 4348, 4627, 4935
Eco47III AGCGCT 3 cut(s) 610, 4998, 5962
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 9 cut(s) 1002, 1477, 1646, 1685, 2619, 3753, 3927, 4592, 5933
Eco72I CACGTG 1 cut(s) 539
Eco81I CCTNAGG 2 cut(s) 1878, 5655
Eco88I CYCGRG 1 cut(s) 868
Eco91I GGTNACC 1 cut(s) 2476
EcoICRI GAGCTC 1 cut(s) 9
EcoNI CCTNNNNNAGG 4 cut(s) 524, 1832, 2108, 4316
EcoO109I RGGNCCY 4 cut(s) 3773, 3894, 4348, 4627
EcoO65I GGTNACC 1 cut(s) 2476
EcoRI GAATTC 4 cut(s) 934, 1141, 2127, 2617
EcoRV GATATC 1 cut(s) 2438
EcoT14I CCWWGG 4 cut(s) 525, 4431, 5399, 5827
EcoT22I ATGCAT 3 cut(s) 1774, 2399, 4795
EcoT38I GRGCYC 2 cut(s) 11, 575
ErhI CCWWGG 4 cut(s) 525, 4431, 5399, 5827
Esp3I CGTCTC 1 cut(s) 5847
FalI AAGNNNNNCTT 4 cut(s) 911, 943, 1619, 1651
FaqI GGGAC 7 cut(s) 449, 887, 986, 3747, 4334, 5507, 6131
FauI CCCGC 4 cut(s) 581, 5182, 5864, 6108
FauNDI CATATG 2 cut(s) 1056, 5226
FblI GTMKAC 1 cut(s) 5359
FriOI GRGCYC 2 cut(s) 11, 575
FspI TGCGCA 1 cut(s) 3977
GsaI CCCAGC 4 cut(s) 1731, 3680, 3684, 4635
GsuI CTGGAG 5 cut(s) 457, 1092, 2552, 4299, 4865
HaeII RGCGCY 5 cut(s) 612, 4149, 5000, 5964, 6220
HapII CCGG 6 cut(s) 869, 953, 3386, 3609, 4933, 6129
HgaI GACGC 2 cut(s) 308, 6049
HindIII AAGCTT 5 cut(s) 3562, 3953, 4718, 5034, 5945
HpaI GTTAAC 3 cut(s) 2469, 2764, 3415
HpaII CCGG 6 cut(s) 869, 953, 3386, 3609, 4933, 6129
Hpy99I CGWCG 3 cut(s) 4521, 5083, 6065
HpyCH4IV ACGT 9 cut(s) 538, 1341, 1458, 1562, 3961, 4519, 5593, 5840, 6143
HpySE526I ACGT 9 cut(s) 538, 1341, 1458, 1562, 3961, 4519, 5593, 5840, 6143
KpnI GGTACC 1 cut(s) 309
KroI GCCGGC 1 cut(s) 3385
KroNI GCCGGC 1 cut(s) 3387
KspAI GTTAAC 3 cut(s) 2469, 2764, 3415
KspI CCGCGG 1 cut(s) 5860
LguI GCTCTTC 1 cut(s) 2608
LmnI GCTCC 8 cut(s) 67, 428, 476, 644, 1876, 3639, 4460, 5659
MabI ACCWGGT 2 cut(s) 1827, 2707
MaeII ACGT 9 cut(s) 538, 1341, 1458, 1562, 3961, 4519, 5593, 5840, 6143
MbiI CCGCTC 2 cut(s) 588, 6115
MfeI CAATTG 4 cut(s) 2024, 2962, 3405, 4562
MflI RGATCY 3 cut(s) 160, 2357, 3970
MhlI GDGCHC 7 cut(s) 11, 575, 1277, 1595, 4457, 5305, 5410
MlsI TGGCCA 2 cut(s) 524, 1692
MluNI TGGCCA 2 cut(s) 524, 1692
MlyI GAGTC 7 cut(s) 1289, 1355, 2105, 2394, 4719, 4984, 5239
MmeI TCCRAC 4 cut(s) 769, 2680, 4441, 5732
Mox20I TGGCCA 2 cut(s) 524, 1692
Mph1103I ATGCAT 3 cut(s) 1774, 2399, 4795
MroNI GCCGGC 1 cut(s) 3385
MroXI GAANNNNTTC 1 cut(s) 2269
MscI TGGCCA 2 cut(s) 524, 1692
MslI CAYNNNNRTG 5 cut(s) 2949, 3084, 3312, 5685, 6168
Msp20I TGGCCA 2 cut(s) 524, 1692
MspA1I CMGCKG 7 cut(s) 1586, 2214, 2958, 3680, 5132, 5189, 5859
MspCI CTTAAG 2 cut(s) 3629, 4376
MspI CCGG 6 cut(s) 869, 953, 3386, 3609, 4933, 6129
MunI CAATTG 4 cut(s) 2024, 2962, 3405, 4562
Mva1269I GAATGC 5 cut(s) 177, 844, 1772, 2397, 4793
MvnI CGCG 2 cut(s) 3234, 5859
NaeI GCCGGC 1 cut(s) 3387
NciI CCSGG 4 cut(s) 869, 870, 954, 6129
NcoI CCATGG 1 cut(s) 4431
NdeI CATATG 2 cut(s) 1056, 5226
NgoMIV GCCGGC 1 cut(s) 3385
NheI GCTAGC 1 cut(s) 2714
NmuCI GTSAC 7 cut(s) 249, 373, 532, 539, 1021, 2686, 5006
NsbI TGCGCA 1 cut(s) 3977
NsiI ATGCAT 3 cut(s) 1774, 2399, 4795
NspV TTCGAA 2 cut(s) 3341, 4655
PaeI GCATGC 2 cut(s) 1934, 5062
PaqCI CACCTGC 3 cut(s) 426, 2455, 2777
PciI ACATGT 3 cut(s) 819, 2031, 3758
PciSI GCTCTTC 1 cut(s) 2608
PcsI WCGNNNNNNNCGW 2 cut(s) 38, 179
PctI GAATGC 5 cut(s) 177, 844, 1772, 2397, 4793
PdiI GCCGGC 1 cut(s) 3387
PdmI GAANNNNTTC 1 cut(s) 2269
PflFI GACNNNGTC 1 cut(s) 545
PflMI CCANNNNNTGG 1 cut(s) 4037
PfoI TCCNGGA 3 cut(s) 952, 1833, 5248
PleI GAGTC 7 cut(s) 1288, 1354, 2104, 2393, 4719, 4984, 5239
PmaCI CACGTG 1 cut(s) 539
PmlI CACGTG 1 cut(s) 539
PpsI GAGTC 7 cut(s) 1288, 1354, 2104, 2393, 4719, 4984, 5239
Ppu21I YACGTR 3 cut(s) 539, 3962, 6144
PpuMI RGGWCCY 3 cut(s) 3773, 4348, 4627
PscI ACATGT 3 cut(s) 819, 2031, 3758
PshBI ATTAAT 1 cut(s) 5685
Psp124BI GAGCTC 1 cut(s) 11
Psp1406I AACGTT 1 cut(s) 1458
Psp5II RGGWCCY 3 cut(s) 3773, 4348, 4627
PspCI CACGTG 1 cut(s) 539
PspEI GGTNACC 1 cut(s) 2476
PspFI CCCAGC 4 cut(s) 1727, 3676, 3680, 4631
PspPPI RGGWCCY 3 cut(s) 3773, 4348, 4627
PsrI GAACNNNNNNTAC 2 cut(s) 4843, 4875
PstI CTGCAG 3 cut(s) 510, 616, 1673
PstNI CAGNNNCTG 1 cut(s) 998
PsuI RGATCY 3 cut(s) 160, 2357, 3970
PsyI GACNNNGTC 1 cut(s) 545
PvuII CAGCTG 5 cut(s) 1586, 2214, 2958, 3680, 5132
RseI CAYNNNNRTG 5 cut(s) 2949, 3084, 3312, 5685, 6168
SacI GAGCTC 1 cut(s) 11
SacII CCGCGG 1 cut(s) 5860
SapI GCTCTTC 1 cut(s) 2608
SchI GAGTC 7 cut(s) 1289, 1355, 2105, 2394, 4719, 4984, 5239
SduI GDGCHC 7 cut(s) 11, 575, 1277, 1595, 4457, 5305, 5410
SexAI ACCWGGT 2 cut(s) 1827, 2707
SfcI CTRYAG 6 cut(s) 506, 612, 1669, 2050, 2719, 4170
Sfr303I CCGCGG 1 cut(s) 5860
SfuI TTCGAA 2 cut(s) 3341, 4655
SgrBI CCGCGG 1 cut(s) 5860
SinI GGWCC 8 cut(s) 517, 1831, 2252, 3238, 3773, 4348, 4627, 4935
SmaI CCCGGG 1 cut(s) 870
SmiI ATTTAAAT 1 cut(s) 3594
SmiMI CAYNNNNRTG 5 cut(s) 2949, 3084, 3312, 5685, 6168
SmlI CTYRAG 6 cut(s) 583, 1208, 3565, 3629, 4376, 4992
SmoI CTYRAG 6 cut(s) 583, 1208, 3565, 3629, 4376, 4992
SnaBI TACGTA 1 cut(s) 6144
SpeI ACTAGT 3 cut(s) 752, 1985, 3296
SphI GCATGC 2 cut(s) 1934, 5062
SsiI CCGC 8 cut(s) 426, 588, 4242, 5189, 5349, 5857, 5859, 6115
SspI AATATT 5 cut(s) 652, 1516, 3364, 4951, 5461
SstI GAGCTC 1 cut(s) 11
StyI CCWWGG 4 cut(s) 525, 4431, 5399, 5827
SwaI ATTTAAAT 1 cut(s) 3594
TaiI ACGT 9 cut(s) 541, 1344, 1461, 1565, 3964, 4522, 5596, 5843, 6146
TaqI TCGA 8 cut(s) 32, 173, 455, 2540, 3341, 4109, 4516, 4655
TatI WGTACW 8 cut(s) 166, 1159, 1943, 2200, 3784, 4123, 5805, 5952
TseFI GTSAC 7 cut(s) 249, 373, 532, 539, 1021, 2686, 5006
Tsp45I GTSAC 7 cut(s) 249, 373, 532, 539, 1021, 2686, 5006
TspGWI ACGGA 3 cut(s) 171, 3825, 4447
TspMI CCCGGG 1 cut(s) 868
Tth111I GACNNNGTC 1 cut(s) 545
Van91I CCANNNNNTGG 1 cut(s) 4037
Vha464I CTTAAG 2 cut(s) 3629, 4376
VneI GTGCAC 2 cut(s) 1273, 5301
VpaK11BI GGWCC 8 cut(s) 517, 1831, 2252, 3238, 3773, 4348, 4627, 4935
VspI ATTAAT 1 cut(s) 5685
XagI CCTNNNNNAGG 4 cut(s) 524, 1832, 2108, 4316
XbaI TCTAGA 3 cut(s) 2261, 4334, 4756
XcmI CCANNNNNNNNNTGG 1 cut(s) 1825
XmaI CCCGGG 1 cut(s) 868
XmiI GTMKAC 1 cut(s) 5359
XmnI GAANNNNTTC 1 cut(s) 2269
Zsp2I ATGCAT 3 cut(s) 1774, 2399, 4795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.