FvH4_2g30651

Predicted integral membrane zinc-ribbon metal-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010402
Physical Location & Seq
Reverse (-)
23703199 .. 23706910
3712 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g30651.t1

Sequence Viewer

Length: 1263 bp
ATGGCGGATGATAAGGACAAAGGCGCTGAAGGCGAGAAGAACGACGCCGTCCCGGCGGTGCCGAAGAAGAAGGGGAAGGGATTCTTCGCCAGAATGCTTACCGGAATGTTTCGGCACGGCGACGATTTCGAGAAGCGGCTGCAGTATCTCTCCAAGGAGGAGGTAACTGTGGTGAACAGAATCAACCGGAGGGCTCTCAATCGGAGAAGCTTCACCAGAAACGTTGTTGCCTTCTCTATCTTCTTCGAGGTTATTGCAATTGGTTATGCTATTGCCACAACGAGAACCACTGACTTGGACTGGAGGACGAGGACATTTCGAATTTTGCCCATGTTTCTCTTTCCTGCGTTGTCCAGTCTTACGTATTCAACATTTACGAACTACACAAGGATGCGTGATCGCAAGGATAATAAGACACTGGAAAGGCTCCGGGCTGAAAAGCAAGCAAAAATTGATGAACTTAAAGAGAAGACCAACTATTACACTACTCAACAGCTTATTCAGAAATATGATGCTGACCCAGCAGCAAAGGCAGCTGCTGCAACTGTCCTGGCATCTAAGTTGGGTGCTGATTCAGGCCTACGATTCCATGTAGGTGATGAATCTAGAGCGAACGCCCCATCTGGGAGAAGCAATGATGTTGAACTTGTACATCCTGGTGAACTTCGGAACCGAAAAACAGGACTGACCAGATCTACCAGTACAGGGAGTGCACATATGCTTTATCCTGAGCAAGAGACTCCTCAGTCTGTTGGGTCAGAGGGTCCCCAGACTGCTGAGCATGGTCAGCTGGTAGTTGCTCATCAGAATCCTCAATCATATGCTACACAAGATGGAAATTGGATTGCTCGTTTAGCGGCATTACTTGTGGGCGAGGATCCTACACAATCTTATGCCCTCATATGTGGCAATTGTCATATGCACAATGGGCTTGCTAAGAAGGAGGATTTCGCATACATCACTTATTATTGCCCACACTGTCATGCTCTAAATAGGCCAAAACATTTGGAAGGGGCCGGTACTGGTTCTAGCTCTCCTGATGCGGGATCTATGATAGCAGGGGACGGGAGTTCTGCAGCAAACCAAGCCAGTGGTTCTTTGAATGACAGTGTAGTTACAGGTACTAGCCCGGTCGGAGCGGGTACCAGCCCTGTCGCAGCAGGTACCAGCCCTGTTGCAGCAGGTCATGAGATTGAGCAAATAAATGAGGTGCTTGTGTCGGGTGATACAGATACAGTTAGTTTAGAAGAAAAACACGAGTAG

Protein Analysis

421

Amino Acids

45.69

Weight (kDa)

6.91

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_10 PF10058 281 - 331 4.2e-20 ER junction formation factor lunapark, zinc ribbon finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 745
Acc36I ACCTGC 2 cut(s) 1151, 1172
Acc65I GGTACC 2 cut(s) 1142, 1163
AccB1I GGYRCC 3 cut(s) 58, 1142, 1163
AccBSI CCGCTC 1 cut(s) 1139
AciI CCGC 6 cut(s) 5, 56, 136, 857, 1043, 1139
AclI AACGTT 1 cut(s) 222
AclWI GGATC 3 cut(s) 872, 885, 1054
AcsI RAATTY 1 cut(s) 321
AcuI CTGAAG 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 6 cut(s) 651, 703, 1021, 1123, 1144, 1165
AfiI CCNNNNNNNGG 3 cut(s) 624, 705, 1043
AgsI TTSAA 3 cut(s) 369, 644, 1102
AjnI CCWGG 2 cut(s) 549, 655
AluBI AGCT 5 cut(s) 210, 496, 536, 790, 1032
AluI AGCT 5 cut(s) 210, 496, 536, 790, 1032
Alw21I GWGCWC 1 cut(s) 715
Alw26I GTCTC 1 cut(s) 731
Alw44I GTGCAC 1 cut(s) 711
AlwI GGATC 3 cut(s) 872, 885, 1054
AlwNI CAGNNNCTG 1 cut(s) 539
AoxI GGCC 3 cut(s) 577, 995, 1014
ApaLI GTGCAC 1 cut(s) 711
ApeKI GCWGC 8 cut(s) 139, 524, 533, 536, 539, 1076, 1157, 1178
ApoI RAATTY 1 cut(s) 321
Asp700I GAANNNNTTC 1 cut(s) 80
Asp718I GGTACC 2 cut(s) 1142, 1163
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 2 cut(s) 764, 1014
AsuC2I CCSGG 3 cut(s) 53, 431, 1130
AsuHPI GGTGA 5 cut(s) 184, 205, 608, 671, 1235
AsuII TTCGAA 1 cut(s) 319
AvaII GGWCC 1 cut(s) 764
BaeGI GKGCMC 1 cut(s) 715
BamHI GGATCC 1 cut(s) 877
BanI GGYRCC 3 cut(s) 58, 1142, 1163
BanII GRGCYC 1 cut(s) 196
BauI CACGAG 1 cut(s) 1256
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 715
BbvI GCAGC 8 cut(s) 126, 523, 526, 536, 545, 1088, 1169, 1190
BccI CCATC 2 cut(s) 628, 827
BceAI ACGGC 2 cut(s) 32, 133
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BciT130I CCWGG 2 cut(s) 551, 657
BcnI CCSGG 3 cut(s) 53, 431, 1130
BcoDI GTCTC 1 cut(s) 731
BfaI CTAG 3 cut(s) 606, 1029, 1125
BfmI CTRYAG 2 cut(s) 140, 1074
BfoI RGCGCY 1 cut(s) 27
BfuAI ACCTGC 2 cut(s) 1151, 1172
BglI GCCNNNNNGGC 1 cut(s) 53
BglII AGATCT 1 cut(s) 692
BlpI GCTNAGC 1 cut(s) 777
Bme1390I CCNGG 5 cut(s) 53, 431, 551, 657, 1130
Bme18I GGWCC 1 cut(s) 764
BmgT120I GGNCC 2 cut(s) 764, 1014
BmiI GGNNCC 9 cut(s) 60, 428, 671, 765, 766, 879, 1015, 1144, 1165
BmrFI CCNGG 5 cut(s) 53, 431, 551, 657, 1130
BmsI GCATC 4 cut(s) 381, 502, 563, 1030
BpiI GAAGAC 1 cut(s) 476
BpmI CTGGAG 1 cut(s) 322
Bpu10I CCTNAGC 1 cut(s) 729
Bpu1102I GCTNAGC 1 cut(s) 777
Bpu14I TTCGAA 1 cut(s) 319
BpuMI CCSGG 3 cut(s) 53, 431, 1130
BsaAI YACGTR 1 cut(s) 363
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 1 cut(s) 153
BsaWI WCCGGW 2 cut(s) 101, 186
Bsc4I CCNNNNNNNGG 3 cut(s) 624, 705, 1043
Bse118I RCCGGY 1 cut(s) 1016
Bse1I ACTGG 6 cut(s) 305, 354, 423, 699, 1027, 1089
Bse3DI GCAATG 1 cut(s) 640
BseBI CCWGG 2 cut(s) 551, 657
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 3 cut(s) 13, 396, 652
BseLI CCNNNNNNNGG 3 cut(s) 624, 705, 1043
BseMI GCAATG 1 cut(s) 640
BseMII CTCAG 3 cut(s) 720, 758, 768
BseNI ACTGG 6 cut(s) 305, 354, 423, 699, 1027, 1089
BseRI GAGGAG 2 cut(s) 173, 732
BseSI GKGCMC 1 cut(s) 715
BseXI GCAGC 8 cut(s) 126, 523, 526, 536, 545, 1088, 1169, 1190
BseYI CCCAGC 1 cut(s) 520
Bsh1285I CGRYCG 1 cut(s) 1134
BshFI GGCC 3 cut(s) 579, 997, 1016
BshNI GGYRCC 3 cut(s) 58, 1142, 1163
BsiEI CGRYCG 1 cut(s) 1134
BsiHKAI GWGCWC 1 cut(s) 715
BsiSI CCGG 6 cut(s) 53, 102, 187, 430, 1017, 1130
BslFI GGGAC 3 cut(s) 35, 750, 1076
BslI CCNNNNNNNGG 3 cut(s) 624, 705, 1043
BsmAI GTCTC 1 cut(s) 731
BsmFI GGGAC 3 cut(s) 35, 750, 1076
BsmI GAATGC 1 cut(s) 99
BsnI GGCC 3 cut(s) 579, 997, 1016
Bsp119I TTCGAA 1 cut(s) 319
Bsp1286I GDGCHC 2 cut(s) 196, 715
Bsp1407I TGTACA 1 cut(s) 649
Bsp143I GATC 4 cut(s) 397, 692, 877, 1046
Bsp1720I GCTNAGC 1 cut(s) 777
BspACI CCGC 6 cut(s) 5, 56, 136, 857, 1043, 1139
BspANI GGCC 3 cut(s) 579, 997, 1016
BspCNI CTCAG 3 cut(s) 721, 757, 769
BspHI TCATGA 1 cut(s) 1186
BspLI GGNNCC 9 cut(s) 60, 428, 671, 765, 766, 879, 1015, 1144, 1165
BspMAI CTGCAG 2 cut(s) 144, 1078
BspMI ACCTGC 2 cut(s) 1151, 1172
BspPI GGATC 3 cut(s) 872, 885, 1054
BspT104I TTCGAA 1 cut(s) 319
BspT107I GGYRCC 3 cut(s) 58, 1142, 1163
BsrBI CCGCTC 1 cut(s) 1139
BsrDI GCAATG 1 cut(s) 640
BsrFI RCCGGY 1 cut(s) 1016
BsrGI TGTACA 1 cut(s) 649
BsrI ACTGG 6 cut(s) 305, 354, 423, 699, 1027, 1089
BssAI RCCGGY 1 cut(s) 1016
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 4 cut(s) 397, 692, 877, 1046
BssNI GRCGYC 1 cut(s) 45
BssSI CACGAG 1 cut(s) 1256
BssT1I CCWWGG 1 cut(s) 153
Bst2BI CACGAG 1 cut(s) 1256
Bst2UI CCWGG 2 cut(s) 551, 657
Bst4CI ACNGT 5 cut(s) 169, 547, 980, 1109, 1237
BstACI GRCGYC 1 cut(s) 45
BstAPI GCANNNNNTGC 1 cut(s) 539
BstAUI TGTACA 1 cut(s) 649
BstBAI YACGTR 1 cut(s) 363
BstBI TTCGAA 1 cut(s) 319
BstC8I GCNNGC 2 cut(s) 444, 933
BstDEI CTNAG 5 cut(s) 558, 729, 744, 777, 936
BstF5I GGATG 3 cut(s) 13, 396, 652
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 4 cut(s) 400, 695, 880, 1049
BstMAI GTCTC 1 cut(s) 731
BstMBI GATC 4 cut(s) 397, 692, 877, 1046
BstMCI CGRYCG 1 cut(s) 1134
BstNI CCWGG 2 cut(s) 551, 657
BstSCI CCNGG 5 cut(s) 51, 429, 549, 655, 1128
BstSFI CTRYAG 2 cut(s) 140, 1074
BstSLI GKGCMC 1 cut(s) 715
BstSNI TACGTA 1 cut(s) 363
BstV1I GCAGC 8 cut(s) 126, 523, 526, 536, 545, 1088, 1169, 1190
BstV2I GAAGAC 1 cut(s) 476
BstX2I RGATCY 3 cut(s) 692, 877, 1046
BstXI CCANNNNNNTGG 2 cut(s) 295, 1091
BstYI RGATCY 3 cut(s) 692, 877, 1046
BsuRI GGCC 3 cut(s) 579, 997, 1016
BtsCI GGATG 3 cut(s) 13, 396, 652
BtsIMutI CAGTG 5 cut(s) 288, 416, 976, 1096, 1114
BveI ACCTGC 2 cut(s) 1151, 1172
Cac8I GCNNGC 2 cut(s) 444, 933
CaiI CAGNNNCTG 1 cut(s) 539
CciI TCATGA 1 cut(s) 1186
CfoI GCGC 1 cut(s) 26
Cfr10I RCCGGY 1 cut(s) 1016
Cfr13I GGNCC 2 cut(s) 764, 1014
CseI GACGC 1 cut(s) 53
Csp6I GTAC 6 cut(s) 650, 702, 1020, 1122, 1143, 1164
CviAII CATG 5 cut(s) 331, 590, 782, 983, 1187
CviQI GTAC 6 cut(s) 650, 702, 1020, 1122, 1143, 1164
DdeI CTNAG 5 cut(s) 558, 729, 744, 777, 936
DpnI GATC 4 cut(s) 399, 694, 879, 1048
DpnII GATC 4 cut(s) 397, 692, 877, 1046
DrdI GACNNNNNNGTC 1 cut(s) 745
DseDI GACNNNNNNGTC 1 cut(s) 745
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 363
Eco130I CCWWGG 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 579
Eco24I GRGCYC 1 cut(s) 196
Eco47I GGWCC 1 cut(s) 764
Eco57I CTGAAG 1 cut(s) 48
EcoO109I RGGNCCY 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 549, 655
EcoT14I CCWWGG 1 cut(s) 153
EcoT38I GRGCYC 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 5 cut(s) 334, 593, 785, 986, 1190
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 3 cut(s) 35, 750, 1076
FatI CATG 5 cut(s) 330, 589, 781, 982, 1186
FauI CCCGC 2 cut(s) 1036, 1132
FauNDI CATATG 4 cut(s) 717, 820, 902, 918
FokI GGATG 3 cut(s) 20, 403, 639
FriOI GRGCYC 1 cut(s) 196
FspBI CTAG 3 cut(s) 606, 1029, 1125
GlaI GCGC 1 cut(s) 25
GsaI CCCAGC 1 cut(s) 524
GsuI CTGGAG 1 cut(s) 322
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 3 cut(s) 579, 997, 1016
HapII CCGG 6 cut(s) 53, 102, 187, 430, 1017, 1130
HgaI GACGC 1 cut(s) 53
HhaI GCGC 1 cut(s) 26
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 5 cut(s) 334, 593, 785, 986, 1190
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HindIII AAGCTT 1 cut(s) 208
HinfI GANTC 7 cut(s) 81, 180, 572, 585, 602, 739, 808
HpaII CCGG 6 cut(s) 53, 102, 187, 430, 1017, 1130
HphI GGTGA 5 cut(s) 184, 205, 608, 671, 1235
Hpy166II GTNNAC 3 cut(s) 175, 662, 713
Hpy188I TCNGA 6 cut(s) 204, 504, 669, 760, 807, 1136
Hpy188III TCNNGA 5 cut(s) 130, 606, 728, 1037, 1187
Hpy8I GTNNAC 3 cut(s) 175, 662, 713
Hpy99I CGWCG 2 cut(s) 47, 125
HpyAV CCTTC 6 cut(s) 23, 64, 70, 241, 934, 1004
HpyCH4III ACNGT 5 cut(s) 169, 547, 980, 1109, 1237
HpyCH4IV ACGT 2 cut(s) 222, 362
HpyCH4V TGCA 7 cut(s) 142, 257, 542, 713, 922, 1076, 1178
HpyF3I CTNAG 5 cut(s) 558, 729, 744, 777, 936
HpySE526I ACGT 2 cut(s) 222, 362
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 5 cut(s) 334, 593, 785, 986, 1190
HspAI GCGC 1 cut(s) 24
KflI GGGWCCC 1 cut(s) 764
KpnI GGTACC 2 cut(s) 1146, 1167
Kzo9I GATC 4 cut(s) 397, 692, 877, 1046
LmnI GCTCC 2 cut(s) 432, 1136
Lsp1109I GCAGC 8 cut(s) 126, 523, 526, 536, 545, 1088, 1169, 1190
LweI GCATC 4 cut(s) 381, 502, 563, 1030
MaeI CTAG 3 cut(s) 606, 1029, 1125
MaeII ACGT 2 cut(s) 222, 362
MaeIII GTNAC 2 cut(s) 163, 1114
MalI GATC 4 cut(s) 399, 694, 879, 1048
MbiI CCGCTC 1 cut(s) 1139
MboI GATC 4 cut(s) 397, 692, 877, 1046
MboII GAAGA 8 cut(s) 49, 76, 76, 79, 232, 235, 481, 1259
MfeI CAATTG 2 cut(s) 258, 910
MflI RGATCY 3 cut(s) 692, 877, 1046
MhlI GDGCHC 2 cut(s) 196, 715
MluCI AATT 5 cut(s) 258, 321, 450, 838, 910
MlyI GAGTC 1 cut(s) 733
MmeI TCCRAC 1 cut(s) 1114
MroXI GAANNNNTTC 1 cut(s) 80
MseI TTAA 1 cut(s) 462
MslI CAYNNNNRTG 4 cut(s) 389, 594, 657, 981
MspA1I CMGCKG 2 cut(s) 536, 790
MspI CCGG 6 cut(s) 53, 102, 187, 430, 1017, 1130
MspR9I CCNGG 5 cut(s) 53, 431, 551, 657, 1130
MunI CAATTG 2 cut(s) 258, 910
Mva1269I GAATGC 1 cut(s) 99
MvaI CCWGG 2 cut(s) 551, 657
NciI CCSGG 3 cut(s) 53, 431, 1130
NdeI CATATG 4 cut(s) 717, 820, 902, 918
NdeII GATC 4 cut(s) 397, 692, 877, 1046
NlaIII CATG 5 cut(s) 334, 593, 785, 986, 1190
NlaIV GGNNCC 9 cut(s) 60, 428, 671, 765, 766, 879, 1015, 1144, 1165
NspV TTCGAA 1 cut(s) 319
PagI TCATGA 1 cut(s) 1186
PceI AGGCCT 1 cut(s) 579
PctI GAATGC 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 6 cut(s) 81, 180, 572, 585, 602, 808
PflFI GACNNNGTC 1 cut(s) 47
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
Ppu21I YACGTR 1 cut(s) 363
PpuMI RGGWCCY 1 cut(s) 764
Psp1406I AACGTT 1 cut(s) 222
Psp5II RGGWCCY 1 cut(s) 764
Psp6I CCWGG 2 cut(s) 549, 655
PspFI CCCAGC 1 cut(s) 520
PspGI CCWGG 2 cut(s) 549, 655
PspN4I GGNNCC 9 cut(s) 60, 428, 671, 765, 766, 879, 1015, 1144, 1165
PspPI GGNCC 2 cut(s) 764, 1014
PspPPI RGGWCCY 1 cut(s) 764
PstI CTGCAG 2 cut(s) 144, 1078
PstNI CAGNNNCTG 1 cut(s) 539
PsuI RGATCY 3 cut(s) 692, 877, 1046
PsyI GACNNNGTC 1 cut(s) 47
PvuII CAGCTG 2 cut(s) 536, 790
RsaI GTAC 6 cut(s) 651, 703, 1021, 1123, 1144, 1165
RsaNI GTAC 6 cut(s) 650, 702, 1020, 1122, 1143, 1164
RseI CAYNNNNRTG 4 cut(s) 389, 594, 657, 981
SaqAI TTAA 1 cut(s) 462
Sau3AI GATC 4 cut(s) 397, 692, 877, 1046
Sau96I GGNCC 2 cut(s) 764, 1014
SchI GAGTC 1 cut(s) 733
ScrFI CCNGG 5 cut(s) 53, 431, 551, 657, 1130
SduI GDGCHC 2 cut(s) 196, 715
SfaNI GCATC 4 cut(s) 381, 502, 563, 1030
SfcI CTRYAG 2 cut(s) 140, 1074
SfuI TTCGAA 1 cut(s) 319
SinI GGWCC 1 cut(s) 764
SmiMI CAYNNNNRTG 4 cut(s) 389, 594, 657, 981
SnaBI TACGTA 1 cut(s) 363
Sse9I AATT 5 cut(s) 258, 321, 450, 838, 910
SseBI AGGCCT 1 cut(s) 579
SsiI CCGC 6 cut(s) 5, 56, 136, 857, 1043, 1139
SspMI CTAG 3 cut(s) 606, 1029, 1125
StuI AGGCCT 1 cut(s) 579
StyD4I CCNGG 5 cut(s) 51, 429, 549, 655, 1128
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 5 cut(s) 169, 547, 980, 1109, 1237
TaiI ACGT 2 cut(s) 225, 365
TaqI TCGA 3 cut(s) 129, 246, 319
TasI AATT 5 cut(s) 258, 321, 450, 838, 910
TatI WGTACW 2 cut(s) 649, 701
TauI GCSGC 2 cut(s) 139, 860
TfiI GAWTC 6 cut(s) 81, 180, 572, 585, 602, 808
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 5 cut(s) 295, 423, 983, 1096, 1114
TseI GCWGC 8 cut(s) 139, 524, 533, 536, 539, 1076, 1157, 1178
TspDTI ATGAA 2 cut(s) 471, 615
TspRI CASTG 5 cut(s) 295, 423, 983, 1096, 1114
Tth111I GACNNNGTC 1 cut(s) 47
VneI GTGCAC 1 cut(s) 711
VpaK11BI GGWCC 1 cut(s) 764
XapI RAATTY 1 cut(s) 321
XbaI TCTAGA 1 cut(s) 605
XmnI GAANNNNTTC 1 cut(s) 80
XspI CTAG 3 cut(s) 606, 1029, 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.