MD15G1110400.v1.1

Predicted integral membrane zinc-ribbon metal-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010402
Physical Location & Seq
Forward (+)
7718620 .. 7722295
3676 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1110400.v1.1.491

Sequence Viewer

Length: 1230 bp
ATGGCGGAGGATAAAGCCGTCGGAGGAGAAGGCGACAAGAAGGACGCTGCTGCGGCGGCTGCAGCGCCTGCGGTTGCGAAGAAGAAGCGCCAGGGAATTTTGTCCAGAATGTTCAGTGGGATCTTCCGACTACACGGCGACGATTTCGAGAAGCGGCTTCAGTACATTTCGAAAGAGGAAGCCACTGTCCTCGCTAGAATGCAGAGGCGGTCCATTACTTGGAGGCGCATGGCCAGAAATCTCATTCTCTTCTCCGTCTTCTTTGAGGTTATTGCAATTGCTTATGCTATAGTAACGACGAGAGCGACGGATTTAGATTGGAAGATGAGGACATTTCGTATTCTGCCCATGTTTCTTTTGCCTGCTATATCTTCTGTTACTTATTCAGCGTTTATGAGCTTCACAAAGATGCGAGATCGCAAGGACACCAAAACCCTGGAAAGGCTTCGGGCTGAAAGGCAAGCAAAAATTGATGAACTTAAAGAGAGGACGAATTATTACAGTACTCAACAACTGATTCAGAGATATGATCCTGACCCAGCAGCAAAGGCAGCTGCTGCAACTGTCCTGGCATCAAAGCTGGGTACAGATTCAGGCTTGAAGTTTTTTGTGGGAGATGAAACTAAGGGTAATGTCTCAACAGGGAAAAGCCATGATGTTGAACTTGTCCATTCTGGTGAACTTCGAAACCGTAAACCAGTACACACCAGATCTAATAGTACAGGGAGTGTTCCACTGAATCCTCTTGAAGAAGAGACACCTCGTTCTGCAAGGTCCGATGGTGCTCAGACTTCTGAGTATAATCAGCTAGTTGTTAGTCATCATAATCCTCAAGGATCATCTCCACAGGATGGAGGATGGATTGCTCGGATTGCAGCATTACTTGTGGGTGAGGATCCAACACAGTCTTATGCGCTCATATGTGGCAACTGTCATATGCACAATGGGCTTGCTAAGAAGGAAGATTTCCCTTACATCACTTATTTTTGCCCGCACTGTCACGCCTTAAATCAGCCAAAACATCTGGAAGGGAGTACTTCTGGTTCTAACACCCCTAGTTTGGGCAATTTGGGAGCAGGGGTCATCGGTGAGCCGACAAAGCAAGCAAGTGATTCCCTGGATGACAGTGTACTCACAAGTACCAGCCCGGTCAAAGCTGGTTCAGAGATTGAGGAAGTAACCGAAGGGCCTGCATTGGCCGATACAGTTCGTTTAGAAAAACATGAGTAG

Protein Analysis

410

Amino Acids

44.94

Weight (kDa)

8.5

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_10 PF10058 287 - 337 2e-19 ER junction formation factor lunapark, zinc ribbon finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 219, 851
AciI CCGC 7 cut(s) 5, 53, 56, 71, 154, 208, 992
AclWI GGATC 5 cut(s) 128, 524, 844, 890, 903
AcoI YGGCCR 2 cut(s) 231, 1197
AcsI RAATTY 1 cut(s) 96
AcuI CTGAAG 1 cut(s) 143
AfaI GTAC 8 cut(s) 164, 505, 586, 702, 721, 1036, 1131, 1141
AfiI CCNNNNNNNGG 5 cut(s) 219, 441, 851, 1060, 1061
AgsI TTSAA 3 cut(s) 601, 662, 749
AjnI CCWGG 4 cut(s) 90, 435, 567, 1116
AluBI AGCT 5 cut(s) 399, 554, 580, 808, 1157
AluI AGCT 5 cut(s) 399, 554, 580, 808, 1157
Alw21I GWGCWC 1 cut(s) 787
Alw26I GTCTC 2 cut(s) 640, 749
AlwI GGATC 5 cut(s) 128, 524, 844, 890, 903
AlwNI CAGNNNCTG 2 cut(s) 68, 557
AoxI GGCC 3 cut(s) 231, 1187, 1197
ApeKI GCWGC 9 cut(s) 47, 50, 59, 62, 542, 551, 554, 557, 875
ApoI RAATTY 1 cut(s) 96
Asp700I GAANNNNTTC 1 cut(s) 444
AspLEI GCGC 4 cut(s) 67, 90, 228, 916
AspS9I GGNCC 3 cut(s) 210, 774, 1187
AsuC2I CCSGG 1 cut(s) 1148
AsuHPI GGTGA 3 cut(s) 689, 902, 1100
AsuII TTCGAA 2 cut(s) 170, 685
AvaII GGWCC 2 cut(s) 210, 774
BalI TGGCCA 1 cut(s) 233
BamHI GGATCC 1 cut(s) 895
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 787
BbvI GCAGC 9 cut(s) 34, 37, 46, 74, 541, 544, 554, 563, 887
BccI CCATC 3 cut(s) 773, 845, 852
BceAI ACGGC 2 cut(s) 2, 151
BcgI CGANNNNNNTGC 2 cut(s) 1172, 1206
BciT130I CCWGG 4 cut(s) 92, 437, 569, 1118
BcnI CCSGG 1 cut(s) 1148
BcoDI GTCTC 2 cut(s) 640, 749
BfaI CTAG 3 cut(s) 195, 809, 1056
BfmI CTRYAG 2 cut(s) 60, 288
BfoI RGCGCY 2 cut(s) 68, 91
BglII AGATCT 1 cut(s) 710
BmcAI AGTACT 2 cut(s) 505, 1036
Bme1390I CCNGG 5 cut(s) 92, 437, 569, 1118, 1148
Bme18I GGWCC 2 cut(s) 210, 774
BmgT120I GGNCC 3 cut(s) 210, 774, 1187
BmiI GGNNCC 1 cut(s) 897
BmrFI CCNGG 5 cut(s) 92, 437, 569, 1118, 1148
BmsI GCATC 2 cut(s) 399, 581
BpiI GAAGAC 1 cut(s) 250
Bpu14I TTCGAA 2 cut(s) 170, 685
BpuEI CTTGAG 1 cut(s) 816
BpuMI CCSGG 1 cut(s) 1148
BsaJI CCNNGG 3 cut(s) 91, 435, 1116
BsaXI ACNNNNNCTCC 2 cut(s) 1065, 1095
Bsc4I CCNNNNNNNGG 5 cut(s) 219, 441, 851, 1060, 1061
Bse1I ACTGG 1 cut(s) 698
BseBI CCWGG 4 cut(s) 92, 437, 569, 1118
BseDI CCNNGG 3 cut(s) 91, 435, 1116
BseGI GGATG 3 cut(s) 856, 863, 1126
BseLI CCNNNNNNNGG 5 cut(s) 219, 441, 851, 1060, 1061
BseMII CTCAG 2 cut(s) 786, 800
BseNI ACTGG 1 cut(s) 698
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 9 cut(s) 34, 37, 46, 74, 541, 544, 554, 563, 887
BseYI CCCAGC 2 cut(s) 538, 580
BshFI GGCC 3 cut(s) 233, 1189, 1199
BsiHKAI GWGCWC 1 cut(s) 787
BsiSI CCGG 1 cut(s) 1148
BslI CCNNNNNNNGG 5 cut(s) 219, 441, 851, 1060, 1061
BsmAI GTCTC 2 cut(s) 640, 749
BsmI GAATGC 1 cut(s) 204
BsnI GGCC 3 cut(s) 233, 1189, 1199
Bsp119I TTCGAA 2 cut(s) 170, 685
Bsp1286I GDGCHC 1 cut(s) 787
Bsp143I GATC 6 cut(s) 120, 415, 529, 710, 836, 895
BspACI CCGC 7 cut(s) 5, 53, 56, 71, 154, 208, 992
BspANI GGCC 3 cut(s) 233, 1189, 1199
BspCNI CTCAG 2 cut(s) 787, 799
BspLI GGNNCC 1 cut(s) 897
BspMAI CTGCAG 1 cut(s) 64
BspPI GGATC 5 cut(s) 128, 524, 844, 890, 903
BspT104I TTCGAA 2 cut(s) 170, 685
BsrI ACTGG 1 cut(s) 698
BssECI CCNNGG 3 cut(s) 91, 435, 1116
BssMI GATC 6 cut(s) 120, 415, 529, 710, 836, 895
Bst2UI CCWGG 4 cut(s) 92, 437, 569, 1118
Bst4CI ACNGT 9 cut(s) 187, 503, 565, 692, 906, 932, 998, 1127, 1207
Bst6I CTCTTC 2 cut(s) 254, 747
BstAPI GCANNNNNTGC 2 cut(s) 68, 557
BstBI TTCGAA 2 cut(s) 170, 685
BstC8I GCNNGC 7 cut(s) 69, 363, 462, 951, 992, 1104, 1191
BstDEI CTNAG 4 cut(s) 624, 786, 795, 954
BstF5I GGATG 3 cut(s) 856, 863, 1126
BstH2I RGCGCY 2 cut(s) 68, 91
BstHHI GCGC 4 cut(s) 67, 90, 228, 916
BstKTI GATC 6 cut(s) 123, 418, 532, 713, 839, 898
BstMAI GTCTC 2 cut(s) 640, 749
BstMBI GATC 6 cut(s) 120, 415, 529, 710, 836, 895
BstNI CCWGG 4 cut(s) 92, 437, 569, 1118
BstSCI CCNGG 5 cut(s) 90, 435, 567, 1116, 1146
BstSFI CTRYAG 2 cut(s) 60, 288
BstV1I GCAGC 9 cut(s) 34, 37, 46, 74, 541, 544, 554, 563, 887
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 3 cut(s) 120, 710, 895
BstXI CCANNNNNNTGG 1 cut(s) 436
BstYI RGATCY 3 cut(s) 120, 710, 895
BsuRI GGCC 3 cut(s) 233, 1189, 1199
BtsCI GGATG 3 cut(s) 856, 863, 1126
BtsIMutI CAGTG 5 cut(s) 121, 183, 734, 994, 1132
Cac8I GCNNGC 7 cut(s) 69, 363, 462, 951, 992, 1104, 1191
CaiI CAGNNNCTG 2 cut(s) 68, 557
CfoI GCGC 4 cut(s) 67, 90, 228, 916
Cfr13I GGNCC 3 cut(s) 210, 774, 1187
CseI GACGC 1 cut(s) 53
Csp6I GTAC 8 cut(s) 163, 504, 585, 701, 720, 1035, 1130, 1140
CviAII CATG 4 cut(s) 229, 349, 653, 1223
CviQI GTAC 8 cut(s) 163, 504, 585, 701, 720, 1035, 1130, 1140
DdeI CTNAG 4 cut(s) 624, 786, 795, 954
DpnI GATC 6 cut(s) 122, 417, 531, 712, 838, 897
DpnII GATC 6 cut(s) 120, 415, 529, 710, 836, 895
EaeI YGGCCR 2 cut(s) 231, 1197
Eam1104I CTCTTC 2 cut(s) 254, 747
EarI CTCTTC 2 cut(s) 254, 747
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 2 cut(s) 210, 774
Eco57I CTGAAG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 1187
EcoRII CCWGG 4 cut(s) 90, 435, 567, 1116
FaeI CATG 4 cut(s) 232, 352, 656, 1226
FatI CATG 4 cut(s) 228, 348, 652, 1222
FauI CCCGC 1 cut(s) 999
FauNDI CATATG 2 cut(s) 920, 936
FokI GGATG 3 cut(s) 863, 870, 1133
FspBI CTAG 3 cut(s) 195, 809, 1056
GlaI GCGC 4 cut(s) 66, 89, 227, 915
GsaI CCCAGC 2 cut(s) 542, 584
HaeII RGCGCY 2 cut(s) 68, 91
HaeIII GGCC 3 cut(s) 233, 1189, 1199
HapII CCGG 1 cut(s) 1148
HgaI GACGC 1 cut(s) 53
HhaI GCGC 4 cut(s) 67, 90, 228, 916
Hin1II CATG 4 cut(s) 232, 352, 656, 1226
Hin6I GCGC 4 cut(s) 65, 88, 226, 914
HinP1I GCGC 4 cut(s) 65, 88, 226, 914
HinfI GANTC 4 cut(s) 517, 590, 739, 1112
HpaII CCGG 1 cut(s) 1148
HphI GGTGA 3 cut(s) 689, 902, 1100
Hpy166II GTNNAC 4 cut(s) 680, 695, 703, 1130
Hpy188I TCNGA 8 cut(s) 23, 128, 522, 778, 789, 796, 870, 1165
Hpy188III TCNNGA 5 cut(s) 105, 148, 533, 746, 1025
Hpy8I GTNNAC 4 cut(s) 680, 695, 703, 1130
Hpy99I CGWCG 4 cut(s) 23, 143, 301, 310
HpyAV CCTTC 5 cut(s) 23, 34, 952, 1022, 1178
HpyCH4III ACNGT 9 cut(s) 187, 503, 565, 692, 906, 932, 998, 1127, 1207
HpyCH4V TGCA 8 cut(s) 62, 202, 275, 560, 770, 875, 940, 1193
HpyF3I CTNAG 4 cut(s) 624, 786, 795, 954
Hsp92II CATG 4 cut(s) 232, 352, 656, 1226
HspAI GCGC 4 cut(s) 65, 88, 226, 914
Kzo9I GATC 6 cut(s) 120, 415, 529, 710, 836, 895
LmnI GCTCC 1 cut(s) 1073
Lsp1109I GCAGC 9 cut(s) 34, 37, 46, 74, 541, 544, 554, 563, 887
LweI GCATC 2 cut(s) 399, 581
MaeI CTAG 3 cut(s) 195, 809, 1056
MaeIII GTNAC 4 cut(s) 292, 376, 998, 1177
MalI GATC 6 cut(s) 122, 417, 531, 712, 838, 897
MboI GATC 6 cut(s) 120, 415, 529, 710, 836, 895
MfeI CAATTG 1 cut(s) 276
MflI RGATCY 3 cut(s) 120, 710, 895
MhlI GDGCHC 1 cut(s) 787
MlsI TGGCCA 1 cut(s) 233
MluCI AATT 5 cut(s) 96, 276, 468, 493, 1066
MluNI TGGCCA 1 cut(s) 233
MmeI TCCRAC 2 cut(s) 151, 923
Mox20I TGGCCA 1 cut(s) 233
MroXI GAANNNNTTC 1 cut(s) 444
MscI TGGCCA 1 cut(s) 233
MseI TTAA 2 cut(s) 480, 1007
MslI CAYNNNNRTG 2 cut(s) 407, 675
Msp20I TGGCCA 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 1 cut(s) 1148
MspR9I CCNGG 5 cut(s) 92, 437, 569, 1118, 1148
MunI CAATTG 1 cut(s) 276
Mva1269I GAATGC 1 cut(s) 204
MvaI CCWGG 4 cut(s) 92, 437, 569, 1118
NciI CCSGG 1 cut(s) 1148
NdeI CATATG 2 cut(s) 920, 936
NdeII GATC 6 cut(s) 120, 415, 529, 710, 836, 895
NlaIII CATG 4 cut(s) 232, 352, 656, 1226
NlaIV GGNNCC 1 cut(s) 897
NmuCI GTSAC 1 cut(s) 998
NspV TTCGAA 2 cut(s) 170, 685
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PctI GAATGC 1 cut(s) 204
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 4 cut(s) 517, 590, 739, 1112
PflMI CCANNNNNTGG 2 cut(s) 219, 851
Psp6I CCWGG 4 cut(s) 90, 435, 567, 1116
PspFI CCCAGC 2 cut(s) 538, 580
PspGI CCWGG 4 cut(s) 90, 435, 567, 1116
PspN4I GGNNCC 1 cut(s) 897
PspPI GGNCC 3 cut(s) 210, 774, 1187
PsrI GAACNNNNNNTAC 2 cut(s) 1027, 1059
PstI CTGCAG 1 cut(s) 64
PstNI CAGNNNCTG 2 cut(s) 68, 557
PsuI RGATCY 3 cut(s) 120, 710, 895
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 8 cut(s) 164, 505, 586, 702, 721, 1036, 1131, 1141
RsaNI GTAC 8 cut(s) 163, 504, 585, 701, 720, 1035, 1130, 1140
RseI CAYNNNNRTG 2 cut(s) 407, 675
SaqAI TTAA 2 cut(s) 480, 1007
Sau3AI GATC 6 cut(s) 120, 415, 529, 710, 836, 895
Sau96I GGNCC 3 cut(s) 210, 774, 1187
ScaI AGTACT 2 cut(s) 505, 1036
ScrFI CCNGG 5 cut(s) 92, 437, 569, 1118, 1148
SduI GDGCHC 1 cut(s) 787
SetI ASST 8 cut(s) 270, 401, 556, 582, 763, 776, 810, 1159
SfaNI GCATC 2 cut(s) 399, 581
SfcI CTRYAG 2 cut(s) 60, 288
SfuI TTCGAA 2 cut(s) 170, 685
SinI GGWCC 2 cut(s) 210, 774
SmiMI CAYNNNNRTG 2 cut(s) 407, 675
SmlI CTYRAG 1 cut(s) 831
SmoI CTYRAG 1 cut(s) 831
Sse9I AATT 5 cut(s) 96, 276, 468, 493, 1066
SsiI CCGC 7 cut(s) 5, 53, 56, 71, 154, 208, 992
SspMI CTAG 3 cut(s) 195, 809, 1056
StyD4I CCNGG 5 cut(s) 90, 435, 567, 1116, 1146
TaaI ACNGT 9 cut(s) 187, 503, 565, 692, 906, 932, 998, 1127, 1207
TaqI TCGA 3 cut(s) 147, 170, 685
TasI AATT 5 cut(s) 96, 276, 468, 493, 1066
TatI WGTACW 6 cut(s) 162, 503, 700, 719, 1034, 1129
TauI GCSGC 3 cut(s) 56, 59, 157
TfiI GAWTC 4 cut(s) 517, 590, 739, 1112
Tru1I TTAA 2 cut(s) 480, 1007
Tru9I TTAA 2 cut(s) 480, 1007
TscAI CASTG 5 cut(s) 121, 190, 741, 1001, 1132
TseFI GTSAC 1 cut(s) 998
TseI GCWGC 9 cut(s) 47, 50, 59, 62, 542, 551, 554, 557, 875
Tsp45I GTSAC 1 cut(s) 998
TspDTI ATGAA 2 cut(s) 489, 633
TspGWI ACGGA 2 cut(s) 244, 323
TspRI CASTG 5 cut(s) 121, 190, 741, 1001, 1132
Van91I CCANNNNNTGG 2 cut(s) 219, 851
VpaK11BI GGWCC 2 cut(s) 210, 774
XapI RAATTY 1 cut(s) 96
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 3 cut(s) 195, 809, 1056
ZrmI AGTACT 2 cut(s) 505, 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.