MD08G1133800.v1.1

Predicted integral membrane zinc-ribbon metal-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010402
Physical Location & Seq
Reverse (-)
12699138 .. 12703800
4663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1133800.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGGCGGAGGATAAAGCCGTAGGAGGAGAAGGCGACAAGAAGGACGCCGCGGCGGCGGCAGCGCCTGCGGTTGCGAAGAAGAAGCGCCAGGGAATTTTCTCCAGAATGTTCAGTGGGATCTTCCGATTACACCGCGACGATTTCGAGAAGCGGCTTCAGTACATTTCGAAAGAGGAAGCCACTGTCCTCGCTAGAATGCAGAGGCGGACCATTACTTGGAGGCGCATGACCAGACATCTCATTCTCTTCTCCGTCTTCTTTGAGGTTATTGCAATTTTTTATGCTATAGTAACGACGAGAGCGACGGATTTAGATTGGAAGATGAGGACATTTCGTATTCTGCCCATGTTTCTTTTGCCTGCTATATCTTCTGTTTCGTATTCAGCATTTGCCAGCTTCACAAAGATGCGTGATCGCAAGGACACCAAAACCCTGGAAAGGCTTCGAGCTGAAAGGCAAGCAAAAATTGATGAACTTAAAGAGAGGACAAACTATTACACTACTCAACAACTTATTCAGAGATATGATCCTGACCCAGCAGCAAAGGCAGCTGCTGCAACTGTCCTGGCATCTAAGTTGAGTATGGATTCAGGTATGAAATTTTATGTGGGTGATGAATCTAAGGGTAATGCCTCAGCAGGGAAAAGCCATGATGTTGATCTTGTCCACTCTGGTGAACTTCGAAACCGTAAACCAGTACACACCAGATCGAATAGTACCGGGAGTGTCCCGCTGAATCCTCTTGAAGAAGAGACACCTCGTTCTGCAAGGTCTGATGGTCAGACTTCTGAGTATAATCAGCTAGTCGTTAGTCATCATAATCCTCAAGGACCATCTCCACAGGATGGAGGATGGATTGCTCGGATTGCAGCATTACTTGTGGGTGAGGATCCAACACAGTCTTATGCGCTCATATGCGGCAACTGTCATATGCACAACGGGCTTGCTAAGAAGGAAGATTTCCCTTACATCACTTATTTTTGCCCGCACTGCCACGCCTTAAATCAGCCAAAACATTTGGAAGGGCATACTTCTGGTTCTAACACCCCTATGACCCCTAGTTTGGACAATTCAAGAACAGGGGTCAGCAGTGGGCCAACAAAGCAAGCCAGTGATTCTCTGGACGACAGTGCAATCTCAAGTGCCAGCCCGGTCAGAGCTGGTTCAGAGATTGAGGAAGCAACTGAACGGCCTGCGTTGGCCGATACAATTAGTGAAGAAAAACGTGAGTAG

Protein Analysis

411

Amino Acids

45.51

Weight (kDa)

8.85

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_10 PF10058 285 - 335 2e-19 ER junction formation factor lunapark, zinc ribbon finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 216, 845
AccII CGCG 2 cut(s) 50, 135
AclWI GGATC 4 cut(s) 125, 521, 884, 897
AcoI YGGCCR 1 cut(s) 1200
AcsI RAATTY 2 cut(s) 93, 599
AcuI CTGAAG 1 cut(s) 140
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 3 cut(s) 161, 699, 718
AfiI CCNNNNNNNGG 5 cut(s) 216, 438, 639, 845, 1063
AgsI TTSAA 2 cut(s) 746, 1074
AjnI CCWGG 3 cut(s) 87, 432, 564
AleI CACNNNNGTG 1 cut(s) 672
AluBI AGCT 5 cut(s) 396, 449, 551, 802, 1160
AluI AGCT 5 cut(s) 396, 449, 551, 802, 1160
Alw26I GTCTC 1 cut(s) 746
AlwI GGATC 4 cut(s) 125, 521, 884, 897
AlwNI CAGNNNCTG 2 cut(s) 65, 554
AoxI GGCC 3 cut(s) 1094, 1190, 1200
ApeKI GCWGC 6 cut(s) 59, 539, 548, 551, 554, 869
ApoI RAATTY 2 cut(s) 93, 599
ArsI GACNNNNNNTTYG 2 cut(s) 1045, 1077
Asp700I GAANNNNTTC 1 cut(s) 441
AspLEI GCGC 4 cut(s) 64, 87, 225, 910
AspS9I GGNCC 3 cut(s) 207, 830, 1094
AsuC2I CCSGG 2 cut(s) 721, 1151
AsuHPI GGTGA 3 cut(s) 623, 686, 896
AsuII TTCGAA 2 cut(s) 167, 682
AvaII GGWCC 2 cut(s) 207, 830
BamHI GGATCC 1 cut(s) 889
BbsI GAAGAC 1 cut(s) 247
BbvCI CCTCAGC 1 cut(s) 634
BbvI GCAGC 6 cut(s) 71, 538, 541, 551, 560, 881
BccI CCATC 4 cut(s) 770, 839, 841, 846
BceAI ACGGC 2 cut(s) 2, 1205
BciT130I CCWGG 3 cut(s) 89, 434, 566
BcnI CCSGG 2 cut(s) 721, 1151
BcoDI GTCTC 1 cut(s) 746
BfaI CTAG 3 cut(s) 192, 803, 1059
BfmI CTRYAG 1 cut(s) 285
BfoI RGCGCY 2 cut(s) 65, 88
BglI GCCNNNNNGGC 1 cut(s) 53
Bme1390I CCNGG 5 cut(s) 89, 434, 566, 721, 1151
Bme18I GGWCC 2 cut(s) 207, 830
BmgT120I GGNCC 3 cut(s) 207, 830, 1094
BmiI GGNNCC 1 cut(s) 891
BmrFI CCNGG 5 cut(s) 89, 434, 566, 721, 1151
BmsI GCATC 2 cut(s) 396, 578
BpiI GAAGAC 1 cut(s) 247
BpmI CTGGAG 1 cut(s) 85
Bpu10I CCTNAGC 1 cut(s) 634
Bpu14I TTCGAA 2 cut(s) 167, 682
BpuEI CTTGAG 2 cut(s) 810, 1123
BpuMI CCSGG 2 cut(s) 721, 1151
BsaBI GATNNNNATC 1 cut(s) 657
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 3 cut(s) 48, 88, 432
Bsc4I CCNNNNNNNGG 5 cut(s) 216, 438, 639, 845, 1063
Bse1I ACTGG 2 cut(s) 695, 1110
Bse8I GATNNNNATC 1 cut(s) 657
BseBI CCWGG 3 cut(s) 89, 434, 566
BseDI CCNNGG 3 cut(s) 48, 88, 432
BseGI GGATG 2 cut(s) 850, 857
BseJI GATNNNNATC 1 cut(s) 657
BseLI CCNNNNNNNGG 5 cut(s) 216, 438, 639, 845, 1063
BseMII CTCAG 2 cut(s) 648, 780
BseNI ACTGG 2 cut(s) 695, 1110
BseRI GAGGAG 1 cut(s) 39
BseXI GCAGC 6 cut(s) 71, 538, 541, 551, 560, 881
BseYI CCCAGC 1 cut(s) 535
Bsh1236I CGCG 2 cut(s) 50, 135
BshFI GGCC 3 cut(s) 1096, 1192, 1202
BsiSI CCGG 2 cut(s) 720, 1151
BslFI GGGAC 1 cut(s) 713
BslI CCNNNNNNNGG 5 cut(s) 216, 438, 639, 845, 1063
BsmAI GTCTC 1 cut(s) 746
BsmFI GGGAC 1 cut(s) 713
BsmI GAATGC 1 cut(s) 201
BsnI GGCC 3 cut(s) 1096, 1192, 1202
Bsp119I TTCGAA 2 cut(s) 167, 682
Bsp143I GATC 6 cut(s) 117, 412, 526, 658, 707, 889
BspANI GGCC 3 cut(s) 1096, 1192, 1202
BspCNI CTCAG 2 cut(s) 647, 781
BspFNI CGCG 2 cut(s) 50, 135
BspLI GGNNCC 1 cut(s) 891
BspPI GGATC 4 cut(s) 125, 521, 884, 897
BspT104I TTCGAA 2 cut(s) 167, 682
BsrI ACTGG 2 cut(s) 695, 1110
BssECI CCNNGG 3 cut(s) 48, 88, 432
BssMI GATC 6 cut(s) 117, 412, 526, 658, 707, 889
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 3 cut(s) 89, 434, 566
Bst4CI ACNGT 6 cut(s) 184, 562, 689, 900, 926, 1130
Bst6I CTCTTC 2 cut(s) 251, 744
BstACI GRCGYC 1 cut(s) 45
BstAPI GCANNNNNTGC 2 cut(s) 65, 554
BstBI TTCGAA 2 cut(s) 167, 682
BstC8I GCNNGC 9 cut(s) 66, 360, 394, 459, 945, 986, 1107, 1147, 1194
BstDEI CTNAG 5 cut(s) 573, 621, 634, 789, 948
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 2 cut(s) 850, 857
BstFNI CGCG 2 cut(s) 50, 135
BstH2I RGCGCY 2 cut(s) 65, 88
BstHHI GCGC 4 cut(s) 64, 87, 225, 910
BstKTI GATC 6 cut(s) 120, 415, 529, 661, 710, 892
BstMAI GTCTC 1 cut(s) 746
BstMBI GATC 6 cut(s) 117, 412, 526, 658, 707, 889
BstNI CCWGG 3 cut(s) 89, 434, 566
BstSCI CCNGG 5 cut(s) 87, 432, 564, 719, 1149
BstSFI CTRYAG 1 cut(s) 285
BstUI CGCG 2 cut(s) 50, 135
BstV1I GCAGC 6 cut(s) 71, 538, 541, 551, 560, 881
BstV2I GAAGAC 1 cut(s) 247
BstX2I RGATCY 2 cut(s) 117, 889
BstXI CCANNNNNNTGG 1 cut(s) 433
BstYI RGATCY 2 cut(s) 117, 889
BsuRI GGCC 3 cut(s) 1096, 1192, 1202
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 2 cut(s) 850, 857
BtsI GCAGTG 2 cut(s) 988, 1096
BtsIMutI CAGTG 6 cut(s) 118, 180, 988, 1096, 1117, 1135
Cac8I GCNNGC 9 cut(s) 66, 360, 394, 459, 945, 986, 1107, 1147, 1194
CaiI CAGNNNCTG 2 cut(s) 65, 554
CfoI GCGC 4 cut(s) 64, 87, 225, 910
Cfr13I GGNCC 3 cut(s) 207, 830, 1094
Cfr42I CCGCGG 1 cut(s) 51
CseI GACGC 1 cut(s) 53
Csp6I GTAC 3 cut(s) 160, 698, 717
CviAII CATG 3 cut(s) 226, 346, 650
CviQI GTAC 3 cut(s) 160, 698, 717
DdeI CTNAG 5 cut(s) 573, 621, 634, 789, 948
DpnI GATC 6 cut(s) 119, 414, 528, 660, 709, 891
DpnII GATC 6 cut(s) 117, 412, 526, 658, 707, 889
EaeI YGGCCR 1 cut(s) 1200
Eam1104I CTCTTC 2 cut(s) 251, 744
EarI CTCTTC 2 cut(s) 251, 744
EciI GGCGGA 2 cut(s) 20, 220
Eco47I GGWCC 2 cut(s) 207, 830
Eco57I CTGAAG 1 cut(s) 140
EcoRII CCWGG 3 cut(s) 87, 432, 564
FaeI CATG 3 cut(s) 229, 349, 653
FaqI GGGAC 1 cut(s) 713
FatI CATG 3 cut(s) 225, 345, 649
FauI CCCGC 2 cut(s) 738, 993
FauNDI CATATG 2 cut(s) 914, 930
FokI GGATG 2 cut(s) 857, 864
FspBI CTAG 3 cut(s) 192, 803, 1059
GlaI GCGC 4 cut(s) 63, 86, 224, 909
GsaI CCCAGC 1 cut(s) 539
GsuI CTGGAG 1 cut(s) 85
HaeII RGCGCY 2 cut(s) 65, 88
HaeIII GGCC 3 cut(s) 1096, 1192, 1202
HapII CCGG 2 cut(s) 720, 1151
HgaI GACGC 1 cut(s) 53
HhaI GCGC 4 cut(s) 64, 87, 225, 910
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 3 cut(s) 229, 349, 653
Hin6I GCGC 4 cut(s) 62, 85, 223, 908
HinP1I GCGC 4 cut(s) 62, 85, 223, 908
HinfI GANTC 4 cut(s) 587, 617, 736, 1115
HpaII CCGG 2 cut(s) 720, 1151
HphI GGTGA 3 cut(s) 623, 686, 896
Hpy166II GTNNAC 4 cut(s) 667, 677, 692, 700
Hpy188I TCNGA 8 cut(s) 125, 519, 775, 783, 790, 864, 1157, 1168
Hpy188III TCNNGA 6 cut(s) 102, 145, 530, 743, 1074, 1121
Hpy8I GTNNAC 4 cut(s) 667, 677, 692, 700
Hpy99I CGWCG 3 cut(s) 140, 298, 307
HpyAV CCTTC 4 cut(s) 23, 34, 946, 1016
HpyCH4III ACNGT 6 cut(s) 184, 562, 689, 900, 926, 1130
HpyCH4IV ACGT 1 cut(s) 1225
HpyCH4V TGCA 7 cut(s) 199, 272, 557, 767, 869, 934, 1133
HpyF3I CTNAG 5 cut(s) 573, 621, 634, 789, 948
HpySE526I ACGT 1 cut(s) 1225
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 3 cut(s) 229, 349, 653
HspAI GCGC 4 cut(s) 62, 85, 223, 908
KspI CCGCGG 1 cut(s) 51
Kzo9I GATC 6 cut(s) 117, 412, 526, 658, 707, 889
Lsp1109I GCAGC 6 cut(s) 71, 538, 541, 551, 560, 881
LweI GCATC 2 cut(s) 396, 578
MaeI CTAG 3 cut(s) 192, 803, 1059
MaeII ACGT 1 cut(s) 1225
MaeIII GTNAC 1 cut(s) 289
MalI GATC 6 cut(s) 119, 414, 528, 660, 709, 891
MboI GATC 6 cut(s) 117, 412, 526, 658, 707, 889
MflI RGATCY 2 cut(s) 117, 889
MluCI AATT 6 cut(s) 93, 273, 465, 599, 1069, 1209
MmeI TCCRAC 1 cut(s) 917
MroXI GAANNNNTTC 1 cut(s) 441
MseI TTAA 2 cut(s) 477, 1001
MslI CAYNNNNRTG 3 cut(s) 404, 672, 1049
MspA1I CMGCKG 3 cut(s) 50, 551, 733
MspI CCGG 2 cut(s) 720, 1151
MspR9I CCNGG 5 cut(s) 89, 434, 566, 721, 1151
Mva1269I GAATGC 1 cut(s) 201
MvaI CCWGG 3 cut(s) 89, 434, 566
MvnI CGCG 2 cut(s) 50, 135
NciI CCSGG 2 cut(s) 721, 1151
NdeI CATATG 2 cut(s) 914, 930
NdeII GATC 6 cut(s) 117, 412, 526, 658, 707, 889
NlaIII CATG 3 cut(s) 229, 349, 653
NlaIV GGNNCC 1 cut(s) 891
NspV TTCGAA 2 cut(s) 167, 682
OliI CACNNNNGTG 1 cut(s) 672
PcsI WCGNNNNNNNCGW 1 cut(s) 299
PctI GAATGC 1 cut(s) 201
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 4 cut(s) 587, 617, 736, 1115
PflMI CCANNNNNTGG 2 cut(s) 216, 845
Psp6I CCWGG 3 cut(s) 87, 432, 564
PspFI CCCAGC 1 cut(s) 535
PspGI CCWGG 3 cut(s) 87, 432, 564
PspN4I GGNNCC 1 cut(s) 891
PspPI GGNCC 3 cut(s) 207, 830, 1094
PstNI CAGNNNCTG 2 cut(s) 65, 554
PsuI RGATCY 2 cut(s) 117, 889
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 3 cut(s) 161, 699, 718
RsaNI GTAC 3 cut(s) 160, 698, 717
RseI CAYNNNNRTG 3 cut(s) 404, 672, 1049
SacII CCGCGG 1 cut(s) 51
SaqAI TTAA 2 cut(s) 477, 1001
Sau3AI GATC 6 cut(s) 117, 412, 526, 658, 707, 889
Sau96I GGNCC 3 cut(s) 207, 830, 1094
ScrFI CCNGG 5 cut(s) 89, 434, 566, 721, 1151
SfaNI GCATC 2 cut(s) 396, 578
SfcI CTRYAG 1 cut(s) 285
Sfr303I CCGCGG 1 cut(s) 51
SfuI TTCGAA 2 cut(s) 167, 682
SgrBI CCGCGG 1 cut(s) 51
SinI GGWCC 2 cut(s) 207, 830
SmiMI CAYNNNNRTG 3 cut(s) 404, 672, 1049
SmlI CTYRAG 2 cut(s) 825, 1138
SmoI CTYRAG 2 cut(s) 825, 1138
Sse9I AATT 6 cut(s) 93, 273, 465, 599, 1069, 1209
SspMI CTAG 3 cut(s) 192, 803, 1059
StyD4I CCNGG 5 cut(s) 87, 432, 564, 719, 1149
TaaI ACNGT 6 cut(s) 184, 562, 689, 900, 926, 1130
TaiI ACGT 1 cut(s) 1228
TaqI TCGA 5 cut(s) 144, 167, 445, 682, 710
TasI AATT 6 cut(s) 93, 273, 465, 599, 1069, 1209
TatI WGTACW 2 cut(s) 159, 697
TauI GCSGC 6 cut(s) 50, 53, 56, 59, 154, 921
TfiI GAWTC 4 cut(s) 587, 617, 736, 1115
Tru1I TTAA 2 cut(s) 477, 1001
Tru9I TTAA 2 cut(s) 477, 1001
TscAI CASTG 6 cut(s) 118, 187, 995, 1096, 1117, 1135
TseI GCWGC 6 cut(s) 59, 539, 548, 551, 554, 869
TspDTI ATGAA 3 cut(s) 486, 611, 630
TspGWI ACGGA 2 cut(s) 241, 320
TspRI CASTG 6 cut(s) 118, 187, 995, 1096, 1117, 1135
Van91I CCANNNNNTGG 2 cut(s) 216, 845
VpaK11BI GGWCC 2 cut(s) 207, 830
XapI RAATTY 2 cut(s) 93, 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 1117
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 3 cut(s) 192, 803, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.