Rroxscaffold_7G00156910

Predicted integral membrane zinc-ribbon metal-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010402
Physical Location & Seq
Reverse (-)
809512 .. 813219
3708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00156910.1

Sequence Viewer

Length: 1239 bp
ATGGCGGATGACAAAGGCCTTGAAGGCGACAAGAACGAAGCCCCCGCCGCCGCCGCCGCCGCTACGCCTCCGGCGGTGCCGAAGAAGAATCGCAAGGGATTTTTTTCCAGAATGTTTACCGGCATGTTTCGGCACCGGGACGATTTCGAGAAACGGCTGCAGTATCTCTCTAAGGAGGAGATTACTGTGGTCAATAGAATCAACAGGAGGGCTCTGACTCGGAGAAGCTTCGCCAGAAACATCATTGCATTCTCTATTTTTTTCGAGGTCATCGCAATTGGTTATGCTATAGCAACTACGAGAACGACTGACTTGGACTGGAGGACGAGGACATTTCGAATTTTGCCAATGTTTCTGTTGCCTGCTTTGTCTTCTTTTACTTATTCAACATTTATGAACTTCACAAAGATGCGTGATCGCAAAGATAATAAGACCCTGGAAAGGCTCCGGGCTGAAAAGCAAGCAAAAATTGATGAACTTAAAGAGAAGACAAACTATTACTCTACTCAACAGCTTATTCAGAAATATGATGCTGACCCAGCAGCAAAGGCAGCTGCTGCAACTGTCCTGGCATCTAAGTTGGGTGCGGATTCAGGCCTAAGATTCCATGTAGGAGATGAATCTAGAGCTAATGCCCCATCTGGGAGAAGCAAGGATGTTGAACTTGTCCAGTCTGGTGAACTTCGAAACCGGAAAACAGGACTCACCAGATCTAATAGTACAGGGAGTACACAATTGCTTTTTTCTGAGGAAGAGACTCCTCGTTCTGTTGGGTCTGAGGGTCCCCAGACTGCTGAGCATGGTCAGCTGGTTGTTGCTCATCAGAATCCTCAAGCATATGCTGCACAAGATGGAAATTGGATTGCTCGTATTGCAGCATTACTTGTGGGTGAGGATCCAACACAATCTTATGCCCTCATATGTGGCAACTGTCATATGCACAATGGGCTTGCTAAGAAGGAGGATTTCGCATACATTACTTATTATTGCCCACACTGTCATGCTCTAAATAGGCCAAAGCATTTGGAAGGGGGTGTTACTGGTTCAGGCTCTCCTGATTCGGGATCTCTGACAGCAGGGGACAGGAGTGATGTAGCAAACCAGGCCAGTGATTCTTTGAATGACAGTGTCGTCACAGGTACCAGCTCAGTTGTAGCTGGTCCTGAGATTGAGCAAATAACTGAGAGGCTTGTGTCGGGTGATACAGATACAGTTAGTTTAGAAGAAAAACACGAGTAG

Protein Analysis

412

Amino Acids

45.35

Weight (kDa)

7.71

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_10 PF10058 287 - 337 3.9e-20 ER junction formation factor lunapark, zinc ribbon finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1130
Acc65I GGTACC 1 cut(s) 1139
AccB1I GGYRCC 3 cut(s) 76, 132, 1139
AciI CCGC 9 cut(s) 5, 45, 48, 51, 54, 57, 60, 74, 587
AclWI GGATC 3 cut(s) 890, 903, 1072
AcsI RAATTY 1 cut(s) 339
AfaI GTAC 3 cut(s) 721, 730, 1141
AfiI CCNNNNNNNGG 3 cut(s) 441, 642, 1061
AgsI TTSAA 4 cut(s) 23, 387, 662, 1120
AjnI CCWGG 3 cut(s) 435, 567, 1101
AluBI AGCT 7 cut(s) 228, 514, 554, 629, 808, 1146, 1157
AluI AGCT 7 cut(s) 228, 514, 554, 629, 808, 1146, 1157
Alw26I GTCTC 1 cut(s) 749
AlwI GGATC 3 cut(s) 890, 903, 1072
AlwNI CAGNNNCTG 1 cut(s) 557
AoxI GGCC 4 cut(s) 16, 595, 1013, 1104
ApeKI GCWGC 7 cut(s) 157, 542, 551, 554, 557, 842, 875
ApoI RAATTY 1 cut(s) 339
Asp718I GGTACC 1 cut(s) 1139
AspS9I GGNCC 2 cut(s) 782, 1160
AsuC2I CCSGG 2 cut(s) 137, 449
AsuHPI GGTGA 4 cut(s) 689, 697, 902, 1211
AsuII TTCGAA 2 cut(s) 337, 685
AvaII GGWCC 2 cut(s) 782, 1160
BamHI GGATCC 1 cut(s) 895
BanI GGYRCC 3 cut(s) 76, 132, 1139
BanII GRGCYC 1 cut(s) 214
BauI CACGAG 1 cut(s) 1232
BbsI GAAGAC 2 cut(s) 363, 494
BbvI GCAGC 7 cut(s) 144, 541, 544, 554, 563, 829, 887
BccI CCATC 2 cut(s) 646, 845
BceAI ACGGC 1 cut(s) 170
BciT130I CCWGG 3 cut(s) 437, 569, 1103
BcnI CCSGG 2 cut(s) 137, 449
BcoDI GTCTC 1 cut(s) 749
BfaI CTAG 1 cut(s) 624
BfmI CTRYAG 2 cut(s) 158, 288
BglI GCCNNNNNGGC 1 cut(s) 24
BglII AGATCT 1 cut(s) 710
BlpI GCTNAGC 1 cut(s) 795
Bme1390I CCNGG 5 cut(s) 137, 437, 449, 569, 1103
Bme18I GGWCC 2 cut(s) 782, 1160
BmgT120I GGNCC 2 cut(s) 782, 1160
BmiI GGNNCC 7 cut(s) 78, 134, 446, 783, 784, 897, 1141
BmrFI CCNGG 5 cut(s) 137, 437, 449, 569, 1103
BmsI GCATC 3 cut(s) 399, 520, 581
BpiI GAAGAC 2 cut(s) 363, 494
BpmI CTGGAG 1 cut(s) 340
Bpu1102I GCTNAGC 1 cut(s) 795
Bpu14I TTCGAA 2 cut(s) 337, 685
BpuEI CTTGAG 1 cut(s) 816
BpuMI CCSGG 2 cut(s) 137, 449
BsaJI CCNNGG 1 cut(s) 435
BsaWI WCCGGW 1 cut(s) 690
Bsc4I CCNNNNNNNGG 3 cut(s) 441, 642, 1061
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 4 cut(s) 323, 670, 1045, 1107
Bse3DI GCAATG 1 cut(s) 243
BseBI CCWGG 3 cut(s) 437, 569, 1103
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 2 cut(s) 13, 661
BseLI CCNNNNNNNGG 3 cut(s) 441, 642, 1061
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 6 cut(s) 738, 768, 786, 1155, 1161, 1173
BseNI ACTGG 4 cut(s) 323, 670, 1045, 1107
BseRI GAGGAG 2 cut(s) 191, 750
BseXI GCAGC 7 cut(s) 144, 541, 544, 554, 563, 829, 887
BseYI CCCAGC 1 cut(s) 538
BsgI GTGCAG 1 cut(s) 828
BshFI GGCC 4 cut(s) 18, 597, 1015, 1106
BshNI GGYRCC 3 cut(s) 76, 132, 1139
BsiSI CCGG 5 cut(s) 71, 120, 136, 448, 691
BslFI GGGAC 3 cut(s) 152, 768, 1094
BslI CCNNNNNNNGG 3 cut(s) 441, 642, 1061
BsmAI GTCTC 1 cut(s) 749
BsmFI GGGAC 3 cut(s) 152, 768, 1094
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 4 cut(s) 18, 597, 1015, 1106
Bsp119I TTCGAA 2 cut(s) 337, 685
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 4 cut(s) 415, 710, 895, 1064
Bsp1720I GCTNAGC 1 cut(s) 795
BspACI CCGC 9 cut(s) 5, 45, 48, 51, 54, 57, 60, 74, 587
BspANI GGCC 4 cut(s) 18, 597, 1015, 1106
BspCNI CTCAG 6 cut(s) 739, 769, 787, 1156, 1160, 1174
BspLI GGNNCC 7 cut(s) 78, 134, 446, 783, 784, 897, 1141
BspMAI CTGCAG 1 cut(s) 162
BspPI GGATC 3 cut(s) 890, 903, 1072
BspT104I TTCGAA 2 cut(s) 337, 685
BspT107I GGYRCC 3 cut(s) 76, 132, 1139
BsrDI GCAATG 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 4 cut(s) 323, 670, 1045, 1107
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 4 cut(s) 415, 710, 895, 1064
BssSI CACGAG 1 cut(s) 1232
Bst2BI CACGAG 1 cut(s) 1232
Bst2UI CCWGG 3 cut(s) 437, 569, 1103
Bst4CI ACNGT 6 cut(s) 187, 565, 932, 998, 1127, 1213
Bst6I CTCTTC 1 cut(s) 747
BstAPI GCANNNNNTGC 2 cut(s) 557, 842
BstBI TTCGAA 2 cut(s) 337, 685
BstC8I GCNNGC 3 cut(s) 363, 462, 951
BstF5I GGATG 2 cut(s) 13, 661
BstKTI GATC 4 cut(s) 418, 713, 898, 1067
BstMAI GTCTC 1 cut(s) 749
BstMBI GATC 4 cut(s) 415, 710, 895, 1064
BstNI CCWGG 3 cut(s) 437, 569, 1103
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 5 cut(s) 135, 435, 447, 567, 1101
BstSFI CTRYAG 2 cut(s) 158, 288
BstV1I GCAGC 7 cut(s) 144, 541, 544, 554, 563, 829, 887
BstV2I GAAGAC 2 cut(s) 363, 494
BstX2I RGATCY 3 cut(s) 710, 895, 1064
BstYI RGATCY 3 cut(s) 710, 895, 1064
BsuRI GGCC 4 cut(s) 18, 597, 1015, 1106
BtgZI GCGATG 1 cut(s) 256
BtsCI GGATG 2 cut(s) 13, 661
BtsIMutI CAGTG 3 cut(s) 994, 1114, 1132
Cac8I GCNNGC 3 cut(s) 363, 462, 951
CaiI CAGNNNCTG 1 cut(s) 557
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 782, 1160
Csp6I GTAC 3 cut(s) 720, 729, 1140
CviAII CATG 4 cut(s) 124, 608, 800, 1001
CviQI GTAC 3 cut(s) 720, 729, 1140
DpnI GATC 4 cut(s) 417, 712, 897, 1066
DpnII GATC 4 cut(s) 415, 710, 895, 1064
DrdI GACNNNNNNGTC 1 cut(s) 1130
DseDI GACNNNNNNGTC 1 cut(s) 1130
Eam1104I CTCTTC 1 cut(s) 747
EarI CTCTTC 1 cut(s) 747
EciI GGCGGA 1 cut(s) 20
Eco147I AGGCCT 2 cut(s) 18, 597
Eco24I GRGCYC 1 cut(s) 214
Eco47I GGWCC 2 cut(s) 782, 1160
EcoO109I RGGNCCY 1 cut(s) 782
EcoRII CCWGG 3 cut(s) 435, 567, 1101
EcoT38I GRGCYC 1 cut(s) 214
FaeI CATG 4 cut(s) 127, 611, 803, 1004
FaqI GGGAC 3 cut(s) 152, 768, 1094
FatI CATG 4 cut(s) 123, 607, 799, 1000
FauI CCCGC 1 cut(s) 52
FauNDI CATATG 3 cut(s) 838, 920, 936
FokI GGATG 2 cut(s) 20, 668
FriOI GRGCYC 1 cut(s) 214
FspBI CTAG 1 cut(s) 624
GsaI CCCAGC 1 cut(s) 542
GsuI CTGGAG 1 cut(s) 340
HaeIII GGCC 4 cut(s) 18, 597, 1015, 1106
HapII CCGG 5 cut(s) 71, 120, 136, 448, 691
Hin1II CATG 4 cut(s) 127, 611, 803, 1004
HindIII AAGCTT 1 cut(s) 226
HpaII CCGG 5 cut(s) 71, 120, 136, 448, 691
HphI GGTGA 4 cut(s) 689, 697, 902, 1211
Hpy166II GTNNAC 3 cut(s) 117, 680, 731
Hpy188I TCNGA 7 cut(s) 216, 222, 522, 748, 778, 825, 1071
Hpy188III TCNNGA 6 cut(s) 108, 148, 624, 1055, 1062, 1163
Hpy8I GTNNAC 3 cut(s) 117, 680, 731
HpyAV CCTTC 3 cut(s) 17, 952, 1022
HpyCH4III ACNGT 6 cut(s) 187, 565, 932, 998, 1127, 1213
HpyCH4V TGCA 6 cut(s) 160, 248, 560, 845, 875, 940
Hsp92II CATG 4 cut(s) 127, 611, 803, 1004
KflI GGGWCCC 1 cut(s) 782
KpnI GGTACC 1 cut(s) 1143
Kzo9I GATC 4 cut(s) 415, 710, 895, 1064
LmnI GCTCC 1 cut(s) 450
Lsp1109I GCAGC 7 cut(s) 144, 541, 544, 554, 563, 829, 887
LweI GCATC 3 cut(s) 399, 520, 581
MaeI CTAG 1 cut(s) 624
MaeIII GTNAC 2 cut(s) 1036, 1132
MalI GATC 4 cut(s) 417, 712, 897, 1066
MboI GATC 4 cut(s) 415, 710, 895, 1064
MboII GAAGA 6 cut(s) 94, 97, 363, 499, 764, 1235
MfeI CAATTG 2 cut(s) 276, 734
MflI RGATCY 3 cut(s) 710, 895, 1064
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 5 cut(s) 276, 339, 468, 734, 856
MlyI GAGTC 3 cut(s) 211, 696, 751
MmeI TCCRAC 1 cut(s) 923
MseI TTAA 1 cut(s) 480
MslI CAYNNNNRTG 2 cut(s) 407, 999
MspA1I CMGCKG 2 cut(s) 554, 808
MspI CCGG 5 cut(s) 71, 120, 136, 448, 691
MspR9I CCNGG 5 cut(s) 137, 437, 449, 569, 1103
MunI CAATTG 2 cut(s) 276, 734
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 3 cut(s) 437, 569, 1103
NciI CCSGG 2 cut(s) 137, 449
NdeI CATATG 3 cut(s) 838, 920, 936
NdeII GATC 4 cut(s) 415, 710, 895, 1064
NlaIII CATG 4 cut(s) 127, 611, 803, 1004
NlaIV GGNNCC 7 cut(s) 78, 134, 446, 783, 784, 897, 1141
NmuCI GTSAC 1 cut(s) 1132
NspI RCATGY 1 cut(s) 127
NspV TTCGAA 2 cut(s) 337, 685
PceI AGGCCT 2 cut(s) 18, 597
PctI GAATGC 1 cut(s) 248
PfeI GAWTC 8 cut(s) 88, 198, 590, 603, 620, 826, 1058, 1112
PflFI GACNNNGTC 1 cut(s) 1127
PleI GAGTC 3 cut(s) 211, 696, 751
PpsI GAGTC 3 cut(s) 211, 696, 751
PpuMI RGGWCCY 1 cut(s) 782
Psp5II RGGWCCY 1 cut(s) 782
Psp6I CCWGG 3 cut(s) 435, 567, 1101
PspFI CCCAGC 1 cut(s) 538
PspGI CCWGG 3 cut(s) 435, 567, 1101
PspN4I GGNNCC 7 cut(s) 78, 134, 446, 783, 784, 897, 1141
PspPI GGNCC 2 cut(s) 782, 1160
PspPPI RGGWCCY 1 cut(s) 782
PstI CTGCAG 1 cut(s) 162
PstNI CAGNNNCTG 1 cut(s) 557
PsuI RGATCY 3 cut(s) 710, 895, 1064
PsyI GACNNNGTC 1 cut(s) 1127
PvuII CAGCTG 2 cut(s) 554, 808
RsaI GTAC 3 cut(s) 721, 730, 1141
RsaNI GTAC 3 cut(s) 720, 729, 1140
RseI CAYNNNNRTG 2 cut(s) 407, 999
SaqAI TTAA 1 cut(s) 480
Sau3AI GATC 4 cut(s) 415, 710, 895, 1064
Sau96I GGNCC 2 cut(s) 782, 1160
SchI GAGTC 3 cut(s) 211, 696, 751
ScrFI CCNGG 5 cut(s) 137, 437, 449, 569, 1103
SduI GDGCHC 1 cut(s) 214
SetI ASST 9 cut(s) 230, 270, 516, 556, 631, 810, 1141, 1148, 1159
SfaNI GCATC 3 cut(s) 399, 520, 581
SfcI CTRYAG 2 cut(s) 158, 288
SfuI TTCGAA 2 cut(s) 337, 685
SinI GGWCC 2 cut(s) 782, 1160
SmiMI CAYNNNNRTG 2 cut(s) 407, 999
SmlI CTYRAG 1 cut(s) 831
SmoI CTYRAG 1 cut(s) 831
Sse9I AATT 5 cut(s) 276, 339, 468, 734, 856
SseBI AGGCCT 2 cut(s) 18, 597
SsiI CCGC 9 cut(s) 5, 45, 48, 51, 54, 57, 60, 74, 587
SspMI CTAG 1 cut(s) 624
StuI AGGCCT 2 cut(s) 18, 597
StyD4I CCNGG 5 cut(s) 135, 435, 447, 567, 1101
TaaI ACNGT 6 cut(s) 187, 565, 932, 998, 1127, 1213
TaqI TCGA 4 cut(s) 147, 264, 337, 685
TasI AATT 5 cut(s) 276, 339, 468, 734, 856
TatI WGTACW 2 cut(s) 719, 728
TauI GCSGC 5 cut(s) 50, 53, 56, 59, 62
TfiI GAWTC 8 cut(s) 88, 198, 590, 603, 620, 826, 1058, 1112
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 3 cut(s) 1001, 1114, 1132
TseFI GTSAC 1 cut(s) 1132
TseI GCWGC 7 cut(s) 157, 542, 551, 554, 557, 842, 875
Tsp45I GTSAC 1 cut(s) 1132
TspDTI ATGAA 3 cut(s) 410, 489, 633
TspRI CASTG 3 cut(s) 1001, 1114, 1132
Tth111I GACNNNGTC 1 cut(s) 1127
VpaK11BI GGWCC 2 cut(s) 782, 1160
XapI RAATTY 1 cut(s) 339
XbaI TCTAGA 1 cut(s) 623
XceI RCATGY 1 cut(s) 127
XspI CTAG 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.