pycom08g11260

Predicted integral membrane zinc-ribbon metal-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010402
Physical Location & Seq
Reverse (-)
9553932 .. 9558195
4264 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g11260.1

Sequence Viewer

Length: 1635 bp
ATGTTTAAACCGCGGTTGGAGAGCTGGCGGGGGTGGGTCGTGCACTCCAACGAACACGGTCCCTGCGGTCCGAGTGGGTCCCACTCACCGCGTACTCCGGCTTCGCGTTGTCGTTGTTGCGAAAAAAAAACACAACGAAATAAAAAAAAGGAAAGAAATAAAAGAAAACCCCGATTTTTGAAGACCCCCTTTCTCTCCTACTTTTTCCCTCGACACTCTCACTCCATCGCCGATACTCTTTCTCTCTCTCCTCTCCCTCTCTCCGTCTCTCTCTTCATCGATCAGAGATTCAGAGCTCTGCGTTGCCCTCACGGACTTCTCTTCTTTTTGTCGATTTCCATCATTACCCCCAACATCCAGAAAGTTCGAATCGATTCAACCTTCTCTCGATTCGCCATCGCCATTTTCAAGAAAGAGATAGAGAACCGCCAAATTGGTGTAATGGCGGAGGATAAAGCCGTAGGAGGAGAAGGCGACAAGAAGGACGCCGCGGCGGCGGCAGCGCCTGCGGTTGCGAAGAAGAAGCGGCAGGGAATTTTCTCCAGAATGTTCAGTGGGATCTTCCGATTACACCGCGACGATTTCGAGAAGCGGCTTCAGTACATTTCGAAAGAGGAAGCCACTGTCCTCGCTAGAATGCAGAGGCGGACCATTACTTGGAGGCGCATGACCAGACATCTCATTCTCTTCTCCGTCTTCTTTGAGGTTATTGCAATTGTTTATGCTATAGTAACGACGAGAGCGACGGATTTAGATTGGAAGAGGAGGACATTTCGTATTCTGCCCATGTTTCTTTTGCCTGCTATATCTTCTGTTTCGTATTCAGCATTTGTGAGCTTCACAAAGATGCGTGATCGCAAGGACACCAAAACCCTGGAAAGGCTTCGAGCTGAAAGGCAAGCAAAAATTGATGAACTTAAAGAGAGGACAAACTATTACACTACTCAACAACTTATTCAGAGATATGATCCTGACCCAGCAGCAAAGGCAGCTGCTGCAACTGTCCTGGCATCTAAGTTGGGTACGGATTCAGGTATGAAATTTTATGTGGGAGATGAATCTAAGGGTAATGCCTCAACAGGGAAAAGCCATGATGTTGAACTTGTGCACTCTGGTGAACTTCGAAACCGTAAACCAGTACACACCAGATCGAATAGTACCGGGAGTGTCCCGCTGAATCCTCTTGAAGAAGAGACACCTCGTTCTGCAAGGTCTGACGGTCAGACTTCCGAGTATAATCAGCTAGTCGTTAGTCATCATAATCCTCAAGGACCATCTCCACAGGATGGAGGATGGATTGCTCGGATTGCAGCATTACTTGTGGGTGAGGATCCAACACAGTCTTATGCGCTCATATGCGGCAACTGTCATATGCACAACGGGCTTGCTAAGAAGGAAGATTTCCCTTACATCACTTATTTTTGCCCGCACTGCCACGCCTTAAATCAGCCAAAACATTTGGAAGGGCATACTTCTGGTTCTAACACCCCTATGACCCCAACAAAGCAAGCCAGTGATTCTCTGGATGACAGTGCAATCTCAAGTGTCAGCCGGGTCAGAGCTGGTTCAGAGATTGAGGAAGCAACTGAAAGGCCTGCATTGGCCGATACAATTAGTGTAGAAAAACATGAGTAG

Protein Analysis

545

Amino Acids

61.34

Weight (kDa)

9.73

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_10 PF10058 432 - 482 2.9e-19 ER junction formation factor lunapark, zinc ribbon finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 657, 1286
AccII CGCG 5 cut(s) 13, 91, 106, 491, 576
AclWI GGATC 4 cut(s) 566, 962, 1325, 1338
AcoI YGGCCR 1 cut(s) 1602
AcsI RAATTY 2 cut(s) 534, 1040
AcuI CTGAAG 1 cut(s) 581
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 5 cut(s) 94, 602, 1024, 1140, 1159
AfiI CCNNNNNNNGG 4 cut(s) 657, 879, 1080, 1286
AgsI TTSAA 5 cut(s) 181, 378, 409, 1100, 1187
AjnI CCWGG 2 cut(s) 873, 1005
AleI CACNNNNGTG 1 cut(s) 1113
AluBI AGCT 7 cut(s) 24, 296, 837, 890, 992, 1243, 1562
AluI AGCT 7 cut(s) 24, 296, 837, 890, 992, 1243, 1562
Alw21I GWGCWC 3 cut(s) 45, 298, 1110
Alw26I GTCTC 2 cut(s) 271, 1187
Alw44I GTGCAC 2 cut(s) 41, 1106
AlwI GGATC 4 cut(s) 566, 962, 1325, 1338
AlwNI CAGNNNCTG 2 cut(s) 506, 995
AoxI GGCC 2 cut(s) 1592, 1602
ApaLI GTGCAC 2 cut(s) 41, 1106
ApeKI GCWGC 6 cut(s) 500, 980, 989, 992, 995, 1310
ApoI RAATTY 2 cut(s) 534, 1040
Asp700I GAANNNNTTC 2 cut(s) 373, 882
AspLEI GCGC 3 cut(s) 505, 666, 1351
AspS9I GGNCC 5 cut(s) 59, 68, 78, 648, 1271
AsuC2I CCSGG 2 cut(s) 1162, 1553
AsuHPI GGTGA 3 cut(s) 78, 1127, 1337
AsuII TTCGAA 3 cut(s) 367, 608, 1123
AvaII GGWCC 5 cut(s) 59, 68, 78, 648, 1271
BaeGI GKGCMC 2 cut(s) 45, 1110
BamHI GGATCC 1 cut(s) 1330
BanII GRGCYC 1 cut(s) 298
BarI GAAGNNNNNNTAC 2 cut(s) 85, 117
BbsI GAAGAC 2 cut(s) 188, 688
Bbv12I GWGCWC 3 cut(s) 45, 298, 1110
BbvI GCAGC 6 cut(s) 512, 979, 982, 992, 1001, 1322
BccI CCATC 6 cut(s) 233, 347, 404, 1280, 1282, 1287
BceAI ACGGC 1 cut(s) 443
BciT130I CCWGG 2 cut(s) 875, 1007
BcnI CCSGG 2 cut(s) 1162, 1553
BcoDI GTCTC 2 cut(s) 271, 1187
BfaI CTAG 2 cut(s) 633, 1244
BfmI CTRYAG 1 cut(s) 726
BfoI RGCGCY 1 cut(s) 506
BglI GCCNNNNNGGC 1 cut(s) 494
Bme1390I CCNGG 4 cut(s) 875, 1007, 1162, 1553
Bme18I GGWCC 5 cut(s) 59, 68, 78, 648, 1271
BmgT120I GGNCC 5 cut(s) 59, 68, 78, 648, 1271
BmiI GGNNCC 4 cut(s) 61, 79, 80, 1332
BmrFI CCNGG 4 cut(s) 875, 1007, 1162, 1553
BmsI GCATC 2 cut(s) 837, 1019
BpiI GAAGAC 2 cut(s) 188, 688
BpmI CTGGAG 1 cut(s) 526
Bpu14I TTCGAA 3 cut(s) 367, 608, 1123
BpuEI CTTGAG 2 cut(s) 1251, 1525
BpuMI CCSGG 2 cut(s) 1162, 1553
Bsa29I ATCGAT 2 cut(s) 279, 372
BsaBI GATNNNNATC 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 3 cut(s) 11, 489, 873
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 4 cut(s) 657, 879, 1080, 1286
Bse1I ACTGG 2 cut(s) 1136, 1512
Bse8I GATNNNNATC 1 cut(s) 338
BseBI CCWGG 2 cut(s) 875, 1007
BseCI ATCGAT 2 cut(s) 279, 372
BseDI CCNNGG 3 cut(s) 11, 489, 873
BseGI GGATG 4 cut(s) 354, 1291, 1298, 1531
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 4 cut(s) 657, 879, 1080, 1286
BseNI ACTGG 2 cut(s) 1136, 1512
BseRI GAGGAG 3 cut(s) 240, 480, 778
BseSI GKGCMC 2 cut(s) 45, 1110
BseXI GCAGC 6 cut(s) 512, 979, 982, 992, 1001, 1322
BseYI CCCAGC 1 cut(s) 976
Bsh1236I CGCG 5 cut(s) 13, 91, 106, 491, 576
BshFI GGCC 2 cut(s) 1594, 1604
BshVI ATCGAT 2 cut(s) 279, 372
BsiHKAI GWGCWC 3 cut(s) 45, 298, 1110
BsiSI CCGG 3 cut(s) 98, 1161, 1552
BslFI GGGAC 3 cut(s) 45, 64, 1154
BslI CCNNNNNNNGG 4 cut(s) 657, 879, 1080, 1286
BsmAI GTCTC 2 cut(s) 271, 1187
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 3 cut(s) 45, 64, 1154
BsmI GAATGC 1 cut(s) 642
BsnI GGCC 2 cut(s) 1594, 1604
Bsp119I TTCGAA 3 cut(s) 367, 608, 1123
Bsp1286I GDGCHC 3 cut(s) 45, 298, 1110
Bsp143I GATC 6 cut(s) 280, 558, 853, 967, 1148, 1330
BspANI GGCC 2 cut(s) 1594, 1604
BspDI ATCGAT 2 cut(s) 279, 372
BspFNI CGCG 5 cut(s) 13, 91, 106, 491, 576
BspLI GGNNCC 4 cut(s) 61, 79, 80, 1332
BspPI GGATC 4 cut(s) 566, 962, 1325, 1338
BspT104I TTCGAA 3 cut(s) 367, 608, 1123
BsrI ACTGG 2 cut(s) 1136, 1512
BssECI CCNNGG 3 cut(s) 11, 489, 873
BssMI GATC 6 cut(s) 280, 558, 853, 967, 1148, 1330
BssNI GRCGYC 1 cut(s) 486
Bst2UI CCWGG 2 cut(s) 875, 1007
Bst4CI ACNGT 8 cut(s) 59, 625, 1003, 1130, 1220, 1341, 1367, 1532
Bst6I CTCTTC 5 cut(s) 278, 326, 692, 755, 1185
BstACI GRCGYC 1 cut(s) 486
BstAPI GCANNNNNTGC 2 cut(s) 506, 995
BstBI TTCGAA 3 cut(s) 367, 608, 1123
BstC8I GCNNGC 8 cut(s) 26, 507, 801, 900, 1386, 1427, 1509, 1596
BstDEI CTNAG 3 cut(s) 1014, 1062, 1389
BstDSI CCRYGG 2 cut(s) 11, 489
BstF5I GGATG 4 cut(s) 354, 1291, 1298, 1531
BstFNI CGCG 5 cut(s) 13, 91, 106, 491, 576
BstH2I RGCGCY 1 cut(s) 506
BstHHI GCGC 3 cut(s) 505, 666, 1351
BstKTI GATC 6 cut(s) 283, 561, 856, 970, 1151, 1333
BstMAI GTCTC 2 cut(s) 271, 1187
BstMBI GATC 6 cut(s) 280, 558, 853, 967, 1148, 1330
BstNI CCWGG 2 cut(s) 875, 1007
BstSCI CCNGG 4 cut(s) 873, 1005, 1160, 1551
BstSFI CTRYAG 1 cut(s) 726
BstSLI GKGCMC 2 cut(s) 45, 1110
BstUI CGCG 5 cut(s) 13, 91, 106, 491, 576
BstV1I GCAGC 6 cut(s) 512, 979, 982, 992, 1001, 1322
BstV2I GAAGAC 2 cut(s) 188, 688
BstX2I RGATCY 2 cut(s) 558, 1330
BstXI CCANNNNNNTGG 1 cut(s) 874
BstYI RGATCY 2 cut(s) 558, 1330
Bsu15I ATCGAT 2 cut(s) 279, 372
BsuRI GGCC 2 cut(s) 1594, 1604
BsuTUI ATCGAT 2 cut(s) 279, 372
BtgI CCRYGG 2 cut(s) 11, 489
BtgZI GCGATG 2 cut(s) 211, 382
BtsCI GGATG 4 cut(s) 354, 1291, 1298, 1531
BtsI GCAGTG 1 cut(s) 1429
BtsIMutI CAGTG 5 cut(s) 559, 621, 1429, 1519, 1537
Cac8I GCNNGC 8 cut(s) 26, 507, 801, 900, 1386, 1427, 1509, 1596
CaiI CAGNNNCTG 2 cut(s) 506, 995
CfoI GCGC 3 cut(s) 505, 666, 1351
Cfr13I GGNCC 5 cut(s) 59, 68, 78, 648, 1271
Cfr42I CCGCGG 2 cut(s) 14, 492
ClaI ATCGAT 2 cut(s) 279, 372
CpoI CGGWCCG 1 cut(s) 68
CseI GACGC 1 cut(s) 494
Csp6I GTAC 5 cut(s) 93, 601, 1023, 1139, 1158
CspI CGGWCCG 1 cut(s) 68
CviAII CATG 4 cut(s) 667, 787, 1091, 1628
CviQI GTAC 5 cut(s) 93, 601, 1023, 1139, 1158
DdeI CTNAG 3 cut(s) 1014, 1062, 1389
DpnI GATC 6 cut(s) 282, 560, 855, 969, 1150, 1332
DpnII GATC 6 cut(s) 280, 558, 853, 967, 1148, 1330
DraI TTTAAA 1 cut(s) 7
EaeI YGGCCR 1 cut(s) 1602
Eam1104I CTCTTC 5 cut(s) 278, 326, 692, 755, 1185
EarI CTCTTC 5 cut(s) 278, 326, 692, 755, 1185
EciI GGCGGA 2 cut(s) 461, 661
Ecl136II GAGCTC 1 cut(s) 296
Eco147I AGGCCT 1 cut(s) 1594
Eco24I GRGCYC 1 cut(s) 298
Eco47I GGWCC 5 cut(s) 59, 68, 78, 648, 1271
Eco53kI GAGCTC 1 cut(s) 296
Eco57I CTGAAG 1 cut(s) 581
EcoICRI GAGCTC 1 cut(s) 296
EcoO109I RGGNCCY 1 cut(s) 78
EcoRII CCWGG 2 cut(s) 873, 1005
EcoT38I GRGCYC 1 cut(s) 298
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 4 cut(s) 670, 790, 1094, 1631
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 3 cut(s) 45, 64, 1154
FatI CATG 4 cut(s) 666, 786, 1090, 1627
FauI CCCGC 3 cut(s) 21, 1179, 1434
FauNDI CATATG 2 cut(s) 1355, 1371
FokI GGATG 4 cut(s) 341, 1298, 1305, 1538
FriOI GRGCYC 1 cut(s) 298
FspBI CTAG 2 cut(s) 633, 1244
GlaI GCGC 3 cut(s) 504, 665, 1350
GsaI CCCAGC 1 cut(s) 980
GsuI CTGGAG 1 cut(s) 526
HaeII RGCGCY 1 cut(s) 506
HaeIII GGCC 2 cut(s) 1594, 1604
HapII CCGG 3 cut(s) 98, 1161, 1552
HgaI GACGC 1 cut(s) 494
HhaI GCGC 3 cut(s) 505, 666, 1351
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 4 cut(s) 670, 790, 1094, 1631
Hin6I GCGC 3 cut(s) 503, 664, 1349
HinP1I GCGC 3 cut(s) 503, 664, 1349
HinfI GANTC 8 cut(s) 288, 369, 374, 390, 1028, 1058, 1177, 1517
HpaII CCGG 3 cut(s) 98, 1161, 1552
HphI GGTGA 3 cut(s) 78, 1127, 1337
Hpy166II GTNNAC 5 cut(s) 43, 1108, 1118, 1133, 1141
Hpy188III TCNNGA 8 cut(s) 358, 387, 409, 543, 586, 971, 1184, 1523
Hpy8I GTNNAC 5 cut(s) 43, 1108, 1118, 1133, 1141
Hpy99I CGWCG 3 cut(s) 581, 739, 748
HpyAV CCTTC 5 cut(s) 391, 464, 475, 1387, 1457
HpyCH4III ACNGT 8 cut(s) 59, 625, 1003, 1130, 1220, 1341, 1367, 1532
HpyF3I CTNAG 3 cut(s) 1014, 1062, 1389
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 4 cut(s) 670, 790, 1094, 1631
HspAI GCGC 3 cut(s) 503, 664, 1349
KflI GGGWCCC 1 cut(s) 78
KspI CCGCGG 2 cut(s) 14, 492
Kzo9I GATC 6 cut(s) 280, 558, 853, 967, 1148, 1330
Lsp1109I GCAGC 6 cut(s) 512, 979, 982, 992, 1001, 1322
LweI GCATC 2 cut(s) 837, 1019
MaeI CTAG 2 cut(s) 633, 1244
MaeIII GTNAC 1 cut(s) 730
MalI GATC 6 cut(s) 282, 560, 855, 969, 1150, 1332
MboI GATC 6 cut(s) 280, 558, 853, 967, 1148, 1330
MfeI CAATTG 1 cut(s) 714
MflI RGATCY 2 cut(s) 558, 1330
MhlI GDGCHC 3 cut(s) 45, 298, 1110
MluCI AATT 6 cut(s) 432, 534, 714, 906, 1040, 1611
MmeI TCCRAC 2 cut(s) 72, 1358
MroXI GAANNNNTTC 2 cut(s) 373, 882
MseI TTAA 3 cut(s) 6, 918, 1442
MslI CAYNNNNRTG 3 cut(s) 845, 1113, 1490
MspA1I CMGCKG 4 cut(s) 13, 491, 992, 1174
MspI CCGG 3 cut(s) 98, 1161, 1552
MspR9I CCNGG 4 cut(s) 875, 1007, 1162, 1553
MssI GTTTAAAC 1 cut(s) 7
MunI CAATTG 1 cut(s) 714
Mva1269I GAATGC 1 cut(s) 642
MvaI CCWGG 2 cut(s) 875, 1007
MvnI CGCG 5 cut(s) 13, 91, 106, 491, 576
NciI CCSGG 2 cut(s) 1162, 1553
NdeI CATATG 2 cut(s) 1355, 1371
NdeII GATC 6 cut(s) 280, 558, 853, 967, 1148, 1330
NlaIII CATG 4 cut(s) 670, 790, 1094, 1631
NlaIV GGNNCC 4 cut(s) 61, 79, 80, 1332
NspV TTCGAA 3 cut(s) 367, 608, 1123
OliI CACNNNNGTG 1 cut(s) 1113
PceI AGGCCT 1 cut(s) 1594
PcsI WCGNNNNNNNCGW 1 cut(s) 740
PctI GAATGC 1 cut(s) 642
PdmI GAANNNNTTC 2 cut(s) 373, 882
PfeI GAWTC 8 cut(s) 288, 369, 374, 390, 1028, 1058, 1177, 1517
PflMI CCANNNNNTGG 2 cut(s) 657, 1286
PmeI GTTTAAAC 1 cut(s) 7
PpuMI RGGWCCY 1 cut(s) 78
Psp124BI GAGCTC 1 cut(s) 298
Psp5II RGGWCCY 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 873, 1005
PspFI CCCAGC 1 cut(s) 976
PspGI CCWGG 2 cut(s) 873, 1005
PspN4I GGNNCC 4 cut(s) 61, 79, 80, 1332
PspPI GGNCC 5 cut(s) 59, 68, 78, 648, 1271
PspPPI RGGWCCY 1 cut(s) 78
PstNI CAGNNNCTG 2 cut(s) 506, 995
PsuI RGATCY 2 cut(s) 558, 1330
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 5 cut(s) 94, 602, 1024, 1140, 1159
RsaNI GTAC 5 cut(s) 93, 601, 1023, 1139, 1158
RseI CAYNNNNRTG 3 cut(s) 845, 1113, 1490
Rsr2I CGGWCCG 1 cut(s) 68
RsrII CGGWCCG 1 cut(s) 68
SacI GAGCTC 1 cut(s) 298
SacII CCGCGG 2 cut(s) 14, 492
SaqAI TTAA 3 cut(s) 6, 918, 1442
Sau3AI GATC 6 cut(s) 280, 558, 853, 967, 1148, 1330
Sau96I GGNCC 5 cut(s) 59, 68, 78, 648, 1271
ScrFI CCNGG 4 cut(s) 875, 1007, 1162, 1553
SduI GDGCHC 3 cut(s) 45, 298, 1110
SfaNI GCATC 2 cut(s) 837, 1019
SfcI CTRYAG 1 cut(s) 726
Sfr303I CCGCGG 2 cut(s) 14, 492
SfuI TTCGAA 3 cut(s) 367, 608, 1123
SgrBI CCGCGG 2 cut(s) 14, 492
SinI GGWCC 5 cut(s) 59, 68, 78, 648, 1271
SmiMI CAYNNNNRTG 3 cut(s) 845, 1113, 1490
SmlI CTYRAG 2 cut(s) 1266, 1540
SmoI CTYRAG 2 cut(s) 1266, 1540
Sse9I AATT 6 cut(s) 432, 534, 714, 906, 1040, 1611
SseBI AGGCCT 1 cut(s) 1594
SspMI CTAG 2 cut(s) 633, 1244
SstI GAGCTC 1 cut(s) 298
StuI AGGCCT 1 cut(s) 1594
StyD4I CCNGG 4 cut(s) 873, 1005, 1160, 1551
TaaI ACNGT 8 cut(s) 59, 625, 1003, 1130, 1220, 1341, 1367, 1532
TasI AATT 6 cut(s) 432, 534, 714, 906, 1040, 1611
TatI WGTACW 2 cut(s) 600, 1138
TauI GCSGC 7 cut(s) 491, 494, 497, 500, 529, 595, 1362
TfiI GAWTC 8 cut(s) 288, 369, 374, 390, 1028, 1058, 1177, 1517
Tru1I TTAA 3 cut(s) 6, 918, 1442
Tru9I TTAA 3 cut(s) 6, 918, 1442
TscAI CASTG 5 cut(s) 559, 628, 1436, 1519, 1537
TseI GCWGC 6 cut(s) 500, 980, 989, 992, 995, 1310
TspDTI ATGAA 4 cut(s) 265, 927, 1052, 1071
TspGWI ACGGA 5 cut(s) 253, 327, 682, 761, 1040
TspRI CASTG 5 cut(s) 559, 628, 1436, 1519, 1537
Van91I CCANNNNNTGG 2 cut(s) 657, 1286
VneI GTGCAC 2 cut(s) 41, 1106
VpaK11BI GGWCC 5 cut(s) 59, 68, 78, 648, 1271
XapI RAATTY 2 cut(s) 534, 1040
XcmI CCANNNNNNNNNTGG 1 cut(s) 1519
XmnI GAANNNNTTC 2 cut(s) 373, 882
XspI CTAG 2 cut(s) 633, 1244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.