FvH4_2g34580

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002837
Physical Location & Seq
Reverse (-)
25650167 .. 25653468
3302 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34580.t4

Sequence Viewer

Length: 687 bp
ATGGCGAAACCCGAGACAGTATCATACGAGGAGAGTCGTAGGCAGAGGATGGAGGAAAACAAGAAGAGAATGGAGGCGCTCAACCTGCCTCAGCTTTCTCTTGCTCTTAAAACCTCTTCCTCTCCTAAACCCTCCCCTATGAAGCGGACCCCCAAGCCGAAGGCCGAGAAGCAGATGGTTGGGGTGAGGAGGTCCTCCCGCGTCGCAAATCTTCCTGCTGCTCCTGTCTACAAAGAAATGGATGTTGTTGAGCGAGTGGTGATACCGAGAAGGTATTCTGGTTCTACTTCTAAGCATAGGGATCTGTCGAATAGGGTGTATGCTTCGTATGAAGATAGAACGGATGCTATGGCGAAAGCTGAGAAATTGGAGTCGGATTTGGGGTCTGATTACCCGACCCTTGTGAAAACAATGCTCCAATCACATGTCACTGGTGGATTCTGGCTGGGTCTTCAAGTCCAATTCTGCAAGGATTATCTCTCAAAGAAAGATGAAATTATGACATTGGTAGACGAAGAGGGTAATGAGTACGAGACTATTTACTTGGCCCGGAAGACAGGACTAAGCGGTGGGTGGAAAGGATTTGCAGTAGCTCATGATTTGGTGGATGGGGATGCAGTGGTCTTTCAATTAATCAAGCCTACAACAATGAAGGTGTACATTATAAGGGTGGAAAGAAAGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

229

Amino Acids

25.85

Weight (kDa)

9.46

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 135 - 214 5.5e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 665
Acc36I ACCTGC 1 cut(s) 93
AccI GTMKAC 2 cut(s) 228, 510
AccII CGCG 1 cut(s) 201
AciI CCGC 3 cut(s) 145, 199, 567
AclWI GGATC 1 cut(s) 309
AfaI GTAC 2 cut(s) 530, 659
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 2 cut(s) 455, 629
AluBI AGCT 3 cut(s) 94, 359, 593
AluI AGCT 3 cut(s) 94, 359, 593
Alw26I GTCTC 2 cut(s) 8, 527
AlwI GGATC 1 cut(s) 309
Ama87I CYCGRG 1 cut(s) 11
AoxI GGCC 3 cut(s) 162, 546, 681
ApeKI GCWGC 1 cut(s) 218
AseI ATTAAT 1 cut(s) 632
Asp700I GAANNNNTTC 1 cut(s) 274
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 3 cut(s) 147, 192, 547
AsuC2I CCSGG 1 cut(s) 550
AsuHPI GGTGA 2 cut(s) 196, 271
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 2 cut(s) 147, 192
BbsI GAAGAC 2 cut(s) 443, 560
BbvCI CCTCAGC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 205
BccI CCATC 3 cut(s) 43, 169, 602
BcnI CCSGG 1 cut(s) 550
BcoDI GTCTC 2 cut(s) 8, 527
BfoI RGCGCY 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 93
BisI GCNGC 1 cut(s) 219
BlsI GCNGC 1 cut(s) 220
Bme1390I CCNGG 1 cut(s) 550
Bme18I GGWCC 2 cut(s) 147, 192
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 3 cut(s) 147, 192, 547
BmiI GGNNCC 1 cut(s) 149
BmrFI CCNGG 1 cut(s) 550
BmsI GCATC 2 cut(s) 334, 604
BpiI GAAGAC 2 cut(s) 443, 560
Bpu10I CCTNAGC 1 cut(s) 90
BpuMI CCSGG 1 cut(s) 550
Bse1I ACTGG 1 cut(s) 436
BseGI GGATG 5 cut(s) 54, 247, 349, 613, 619
BseMII CTCAG 2 cut(s) 104, 351
BseNI ACTGG 1 cut(s) 436
BseRI GAGGAG 2 cut(s) 44, 202
BseXI GCAGC 1 cut(s) 205
BseYI CCCAGC 1 cut(s) 445
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 3 cut(s) 164, 548, 683
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 1 cut(s) 550
BsmAI GTCTC 2 cut(s) 8, 527
BsnI GGCC 3 cut(s) 164, 548, 683
BsoBI CYCGRG 1 cut(s) 11
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 1 cut(s) 301
BspACI CCGC 3 cut(s) 145, 199, 567
BspANI GGCC 3 cut(s) 164, 548, 683
BspCNI CTCAG 2 cut(s) 103, 352
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 1 cut(s) 149
BspMI ACCTGC 1 cut(s) 93
BspPI GGATC 1 cut(s) 309
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 1 cut(s) 436
BssMI GATC 1 cut(s) 301
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 3 cut(s) 59, 121, 510
BstAUI TGTACA 1 cut(s) 657
BstDEI CTNAG 4 cut(s) 90, 291, 360, 563
BstF5I GGATG 5 cut(s) 54, 247, 349, 613, 619
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 1 cut(s) 304
BstMAI GTCTC 2 cut(s) 8, 527
BstMBI GATC 1 cut(s) 301
BstMWI GCNNNNNNNGC 1 cut(s) 85
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 1 cut(s) 548
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 1 cut(s) 205
BstV2I GAAGAC 2 cut(s) 443, 560
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BsuRI GGCC 3 cut(s) 164, 548, 683
BtsCI GGATG 5 cut(s) 54, 247, 349, 613, 619
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 2 cut(s) 429, 624
BveI ACCTGC 1 cut(s) 93
CciI TCATGA 1 cut(s) 595
CfoI GCGC 1 cut(s) 79
Cfr13I GGNCC 3 cut(s) 147, 192, 547
CseI GACGC 1 cut(s) 190
Csp6I GTAC 2 cut(s) 529, 658
CviAII CATG 2 cut(s) 425, 596
CviJI RGCY 9 cut(s) 94, 157, 164, 359, 445, 548, 593, 640, 683
CviKI_1 RGCY 9 cut(s) 94, 157, 164, 359, 445, 548, 593, 640, 683
CviQI GTAC 2 cut(s) 529, 658
DdeI CTNAG 4 cut(s) 90, 291, 360, 563
DpnI GATC 1 cut(s) 303
DpnII GATC 1 cut(s) 301
Eam1104I CTCTTC 3 cut(s) 59, 121, 510
EarI CTCTTC 3 cut(s) 59, 121, 510
Eco147I AGGCCT 1 cut(s) 683
Eco47I GGWCC 2 cut(s) 147, 192
Eco88I CYCGRG 1 cut(s) 11
EcoO109I RGGNCCY 1 cut(s) 192
FaeI CATG 2 cut(s) 428, 599
FatI CATG 2 cut(s) 424, 595
FauI CCCGC 1 cut(s) 206
FblI GTMKAC 2 cut(s) 228, 510
Fnu4HI GCNGC 1 cut(s) 219
FokI GGATG 5 cut(s) 61, 254, 356, 620, 626
Fsp4HI GCNGC 1 cut(s) 219
GlaI GCGC 1 cut(s) 78
GluI GCNGC 1 cut(s) 219
GsaI CCCAGC 1 cut(s) 449
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 3 cut(s) 164, 548, 683
HapII CCGG 1 cut(s) 550
HgaI GACGC 1 cut(s) 190
HhaI GCGC 1 cut(s) 79
Hin1II CATG 2 cut(s) 428, 599
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HinfI GANTC 3 cut(s) 34, 371, 438
HpaII CCGG 1 cut(s) 550
HphI GGTGA 2 cut(s) 196, 271
Hpy166II GTNNAC 3 cut(s) 229, 511, 658
Hpy188I TCNGA 2 cut(s) 376, 388
Hpy188III TCNNGA 1 cut(s) 596
Hpy8I GTNNAC 3 cut(s) 229, 511, 658
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 3 cut(s) 154, 264, 646
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4V TGCA 3 cut(s) 468, 587, 617
HpyF10VI GCNNNNNNNGC 1 cut(s) 85
HpyF3I CTNAG 4 cut(s) 90, 291, 360, 563
Hsp92II CATG 2 cut(s) 428, 599
HspAI GCGC 1 cut(s) 77
Kzo9I GATC 1 cut(s) 301
LmnI GCTCC 2 cut(s) 226, 420
LpnPI CCDG 9 cut(s) 98, 228, 237, 264, 417, 427, 431, 543, 563
Lsp1109I GCAGC 1 cut(s) 205
LweI GCATC 2 cut(s) 334, 604
MaeIII GTNAC 1 cut(s) 427
MalI GATC 1 cut(s) 303
MboI GATC 1 cut(s) 301
MboII GAAGA 7 cut(s) 76, 108, 203, 344, 443, 527, 565
MflI RGATCY 1 cut(s) 301
MluCI AATT 4 cut(s) 365, 461, 495, 629
MlyI GAGTC 2 cut(s) 43, 380
MmeI TCCRAC 1 cut(s) 354
MroXI GAANNNNTTC 1 cut(s) 274
MseI TTAA 2 cut(s) 108, 632
MspI CCGG 1 cut(s) 550
MspR9I CCNGG 1 cut(s) 550
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 1 cut(s) 85
NciI CCSGG 1 cut(s) 550
NdeII GATC 1 cut(s) 301
NlaIII CATG 2 cut(s) 428, 599
NlaIV GGNNCC 1 cut(s) 149
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 427
NspI RCATGY 1 cut(s) 428
PagI TCATGA 1 cut(s) 595
PceI AGGCCT 1 cut(s) 683
PciI ACATGT 1 cut(s) 424
PdmI GAANNNNTTC 1 cut(s) 274
PfeI GAWTC 1 cut(s) 438
PkrI GCNGC 1 cut(s) 220
PleI GAGTC 2 cut(s) 42, 379
PpsI GAGTC 2 cut(s) 42, 379
PpuMI RGGWCCY 1 cut(s) 192
PscI ACATGT 1 cut(s) 424
PshBI ATTAAT 1 cut(s) 632
PsiI TTATAA 1 cut(s) 665
Psp5II RGGWCCY 1 cut(s) 192
PspFI CCCAGC 1 cut(s) 445
PspN4I GGNNCC 1 cut(s) 149
PspPI GGNCC 3 cut(s) 147, 192, 547
PspPPI RGGWCCY 1 cut(s) 192
PsuI RGATCY 1 cut(s) 301
RsaI GTAC 2 cut(s) 530, 659
RsaNI GTAC 2 cut(s) 529, 658
SaqAI TTAA 2 cut(s) 108, 632
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 1 cut(s) 301
Sau96I GGNCC 3 cut(s) 147, 192, 547
SchI GAGTC 2 cut(s) 43, 380
ScrFI CCNGG 1 cut(s) 550
SetI ASST 8 cut(s) 87, 96, 116, 194, 275, 361, 595, 657
SfaNI GCATC 2 cut(s) 334, 604
SinI GGWCC 2 cut(s) 147, 192
Sse9I AATT 4 cut(s) 365, 461, 495, 629
SseBI AGGCCT 1 cut(s) 683
SsiI CCGC 3 cut(s) 145, 199, 567
StuI AGGCCT 1 cut(s) 683
StyD4I CCNGG 1 cut(s) 548
TaaI ACNGT 1 cut(s) 19
TaqI TCGA 1 cut(s) 308
TasI AATT 4 cut(s) 365, 461, 495, 629
TatI WGTACW 1 cut(s) 657
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 2 cut(s) 108, 632
Tru9I TTAA 2 cut(s) 108, 632
TscAI CASTG 2 cut(s) 436, 624
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 1 cut(s) 218
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 4 cut(s) 155, 345, 507, 665
TspGWI ACGGA 1 cut(s) 356
TspRI CASTG 2 cut(s) 436, 624
VpaK11BI GGWCC 2 cut(s) 147, 192
VspI ATTAAT 1 cut(s) 632
XceI RCATGY 1 cut(s) 428
XmiI GTMKAC 2 cut(s) 228, 510
XmnI GAANNNNTTC 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.