RchiOBHm_Chr6g0307731

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002837
Physical Location & Seq
Forward (+)
65964298 .. 65965691
1394 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27667

Sequence Viewer

Length: 756 bp
ATGGCTAAACCCAAGACAGTATCATACGAGGAGAGTCGCAAGCAGAGGTTGGAGGAAAACAGGAAGAGAATGGAGGCACTCAATCTGCCTCAGCTTTCTCTCGCTCTTAAAACCTCTTCCTCTCCCAAATCCTCCCCTATGAAGCGAACCCCCAAGCCCAAGGCCGAGAAGCAGATGGTTGTGGTGAGGAGGTCCTCTCGAGTCGCAAATCTACCTTCGCCTCCTGTCTACAAAGAAATGGAAGTTGTTGAGCGAGTTATGATACCGAGAAGGTATTCTGGTTCTACTTCTAAGCATAGGGATCTGTCGAATCGGGTGTATGCTTCTTATGAAGATAGAATGGATGCTATGGCGAAAGCTGAAAAATTGGAGTCGGATTTGGGGTCTGATTACCCGACTCTTGTGAAAACAATGCTCCAATCACATGTCACTGGTGGATTTTGGCTGGGTCTTCAAGTCCAGTTCTGCAAGGATTATCTTTCAAAGAAAGATGAAATTATGACATTGGTAGACGAAGATGGTAATGAGTACGAGACTATTTACTTGGCCCGGAAGACAGGACTAAGTGGTGGATGGAAAGGATTTGCAGTTGCTCATGATTTGGTGGATGGGGATGCAGTGGTCTTTCAATTAATCAAGCCTACAACAATGAAGGTGATTTATTATCACATTTTGATGATAGAGGATACACTAAAACCCTTTAAAGTACAAGACAGATATAGTATACTGGTGGATTATGATAAGGAGTCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

251

Amino Acids

28.81

Weight (kDa)

9.22

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 135 - 215 4.5e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 228, 510, 724
AclWI GGATC 1 cut(s) 309
AfaI GTAC 2 cut(s) 530, 708
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 3 cut(s) 455, 483, 629
AluBI AGCT 2 cut(s) 94, 359
AluI AGCT 2 cut(s) 94, 359
Alw26I GTCTC 1 cut(s) 527
AlwI GGATC 1 cut(s) 309
Ama87I CYCGRG 1 cut(s) 198
AoxI GGCC 2 cut(s) 162, 546
AseI ATTAAT 1 cut(s) 632
Asp700I GAANNNNTTC 1 cut(s) 274
AspS9I GGNCC 2 cut(s) 192, 547
AsuC2I CCSGG 1 cut(s) 550
AsuHPI GGTGA 2 cut(s) 196, 667
AvaI CYCGRG 1 cut(s) 198
AvaII GGWCC 1 cut(s) 192
BbsI GAAGAC 2 cut(s) 443, 560
BbvCI CCTCAGC 1 cut(s) 90
BccI CCATC 4 cut(s) 169, 512, 567, 602
BciVI GTATCC 1 cut(s) 679
BcnI CCSGG 1 cut(s) 550
BcoDI GTCTC 1 cut(s) 527
BfuI GTATCC 1 cut(s) 679
Bme1390I CCNGG 1 cut(s) 550
Bme18I GGWCC 1 cut(s) 192
BmeT110I CYCGRG 1 cut(s) 198
BmgT120I GGNCC 2 cut(s) 192, 547
BmrFI CCNGG 1 cut(s) 550
BmsI GCATC 2 cut(s) 334, 604
BpiI GAAGAC 2 cut(s) 443, 560
BplI GAGNNNNNCTC 2 cut(s) 181, 213
Bpu10I CCTNAGC 1 cut(s) 90
BpuMI CCSGG 1 cut(s) 550
BsaJI CCNNGG 1 cut(s) 159
Bse1I ACTGG 3 cut(s) 436, 460, 732
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 4 cut(s) 349, 578, 613, 619
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 3 cut(s) 436, 460, 732
BseRI GAGGAG 2 cut(s) 44, 202
BseYI CCCAGC 1 cut(s) 445
BshFI GGCC 2 cut(s) 164, 548
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 1 cut(s) 550
BsmAI GTCTC 1 cut(s) 527
BsnI GGCC 2 cut(s) 164, 548
BsoBI CYCGRG 1 cut(s) 198
Bsp143I GATC 1 cut(s) 301
BspANI GGCC 2 cut(s) 164, 548
BspCNI CTCAG 1 cut(s) 103
BspHI TCATGA 1 cut(s) 595
BspPI GGATC 1 cut(s) 309
BsrI ACTGG 3 cut(s) 436, 460, 732
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 1 cut(s) 301
BssNAI GTATAC 1 cut(s) 725
BssT1I CCWWGG 1 cut(s) 159
Bst1107I GTATAC 1 cut(s) 725
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 2 cut(s) 59, 121
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 3 cut(s) 90, 291, 563
BstF5I GGATG 4 cut(s) 349, 578, 613, 619
BstKTI GATC 1 cut(s) 304
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 1 cut(s) 301
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 1 cut(s) 548
BstV2I GAAGAC 2 cut(s) 443, 560
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BstZ17I GTATAC 1 cut(s) 725
BsuI GTATCC 1 cut(s) 679
BsuRI GGCC 2 cut(s) 164, 548
BtsCI GGATG 4 cut(s) 349, 578, 613, 619
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 2 cut(s) 429, 624
Cac8I GCNNGC 1 cut(s) 41
CciI TCATGA 1 cut(s) 595
Cfr13I GGNCC 2 cut(s) 192, 547
Csp6I GTAC 2 cut(s) 529, 707
CviAII CATG 2 cut(s) 425, 596
CviJI RGCY 8 cut(s) 5, 94, 157, 164, 359, 445, 548, 640
CviKI_1 RGCY 8 cut(s) 5, 94, 157, 164, 359, 445, 548, 640
CviQI GTAC 2 cut(s) 529, 707
DdeI CTNAG 3 cut(s) 90, 291, 563
DpnI GATC 1 cut(s) 303
DpnII GATC 1 cut(s) 301
DraI TTTAAA 1 cut(s) 703
Eam1104I CTCTTC 2 cut(s) 59, 121
EarI CTCTTC 2 cut(s) 59, 121
Eco130I CCWWGG 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 198
EcoO109I RGGNCCY 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 2 cut(s) 428, 599
FatI CATG 2 cut(s) 424, 595
FblI GTMKAC 3 cut(s) 228, 510, 724
FokI GGATG 4 cut(s) 356, 585, 620, 626
GsaI CCCAGC 1 cut(s) 449
HaeIII GGCC 2 cut(s) 164, 548
HapII CCGG 1 cut(s) 550
Hin1II CATG 2 cut(s) 428, 599
HinfI GANTC 6 cut(s) 34, 201, 310, 371, 397, 746
HpaII CCGG 1 cut(s) 550
HphI GGTGA 2 cut(s) 196, 667
Hpy166II GTNNAC 3 cut(s) 229, 511, 725
Hpy188I TCNGA 2 cut(s) 376, 388
Hpy188III TCNNGA 2 cut(s) 198, 596
Hpy8I GTNNAC 3 cut(s) 229, 511, 725
HpyAV CCTTC 3 cut(s) 225, 264, 646
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4V TGCA 3 cut(s) 468, 587, 617
HpyF3I CTNAG 3 cut(s) 90, 291, 563
Hsp92II CATG 2 cut(s) 428, 599
Kzo9I GATC 1 cut(s) 301
LmnI GCTCC 1 cut(s) 420
LpnPI CCDG 9 cut(s) 46, 237, 264, 417, 431, 473, 543, 563, 713
LweI GCATC 2 cut(s) 334, 604
MaeIII GTNAC 1 cut(s) 427
MalI GATC 1 cut(s) 303
MboI GATC 1 cut(s) 301
MboII GAAGA 6 cut(s) 76, 108, 344, 443, 527, 565
MflI RGATCY 1 cut(s) 301
MluCI AATT 3 cut(s) 365, 495, 629
MlyI GAGTC 5 cut(s) 43, 210, 380, 391, 755
MmeI TCCRAC 2 cut(s) 30, 354
MroXI GAANNNNTTC 1 cut(s) 274
MseI TTAA 3 cut(s) 108, 632, 702
MslI CAYNNNNRTG 1 cut(s) 674
MspI CCGG 1 cut(s) 550
MspR9I CCNGG 1 cut(s) 550
NciI CCSGG 1 cut(s) 550
NdeII GATC 1 cut(s) 301
NlaIII CATG 2 cut(s) 428, 599
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 427
NspI RCATGY 1 cut(s) 428
PaeR7I CTCGAG 1 cut(s) 198
PagI TCATGA 1 cut(s) 595
PciI ACATGT 1 cut(s) 424
PdmI GAANNNNTTC 1 cut(s) 274
PfeI GAWTC 1 cut(s) 310
PleI GAGTC 5 cut(s) 42, 209, 379, 391, 754
PpsI GAGTC 5 cut(s) 42, 209, 379, 391, 754
PpuMI RGGWCCY 1 cut(s) 192
PscI ACATGT 1 cut(s) 424
PshBI ATTAAT 1 cut(s) 632
Psp5II RGGWCCY 1 cut(s) 192
PspFI CCCAGC 1 cut(s) 445
PspPI GGNCC 2 cut(s) 192, 547
PspPPI RGGWCCY 1 cut(s) 192
PsuI RGATCY 1 cut(s) 301
RsaI GTAC 2 cut(s) 530, 708
RsaNI GTAC 2 cut(s) 529, 707
RseI CAYNNNNRTG 1 cut(s) 674
SaqAI TTAA 3 cut(s) 108, 632, 702
Sau3AI GATC 1 cut(s) 301
Sau96I GGNCC 2 cut(s) 192, 547
SchI GAGTC 5 cut(s) 43, 210, 380, 391, 755
ScrFI CCNGG 1 cut(s) 550
SetI ASST 8 cut(s) 50, 96, 116, 194, 217, 275, 361, 657
SfaNI GCATC 2 cut(s) 334, 604
Sfr274I CTCGAG 1 cut(s) 198
SinI GGWCC 1 cut(s) 192
SlaI CTCGAG 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 674
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 3 cut(s) 365, 495, 629
StyD4I CCNGG 1 cut(s) 548
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 1 cut(s) 19
TaqI TCGA 2 cut(s) 199, 308
TasI AATT 3 cut(s) 365, 495, 629
TatI WGTACW 1 cut(s) 706
TfiI GAWTC 1 cut(s) 310
Tru1I TTAA 3 cut(s) 108, 632, 702
Tru9I TTAA 3 cut(s) 108, 632, 702
TscAI CASTG 2 cut(s) 436, 624
TseFI GTSAC 1 cut(s) 427
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 4 cut(s) 155, 345, 507, 665
TspRI CASTG 2 cut(s) 436, 624
VpaK11BI GGWCC 1 cut(s) 192
VspI ATTAAT 1 cut(s) 632
XceI RCATGY 1 cut(s) 428
XhoI CTCGAG 1 cut(s) 198
XmiI GTMKAC 3 cut(s) 228, 510, 724
XmnI GAANNNNTTC 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.