MD15G1084300.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002837
Physical Location & Seq
Reverse (-)
5830884 .. 5833516
2633 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1084300.v1.1.491

Sequence Viewer

Length: 708 bp
ATGGTGACGGCAAAGTCTAAATTGTCGTACGAGGAGTCTCGCCAGCAGCGATTGGAGGAAAACAAAAAGAGAATGGAAGCTCTCAATCTGCCTATGCTTGCTCAAGCTCTTAACAAAACCCCAAATTCTCCCAAACCCTCCCCTATGAAGCGCGCCGGGAAGCCTCGCACGTATGAGGTGCAGATGGTGGAGGTGAGGAGGTCCACTCGGGTTGCAAATCGACCTGCACCCGATTACAAAGAAGTTGTGGCGGAGCGAGTAATGATTCCGAGAAGGAGCTACTCCACTAATCGGCATAGGGATGTGGCGAACCGGGTGTATGCTTCCGATGAAGCCAGAGCCGCCGCGATGGTGAAAGCCGACGAATTAGAGGCCAGTTTGGGATCCGATCACCCGACACTTGTGAAAACAATGCTGCAATCACATGTCACTGGTGGATTCTGGCTGGGTCTTCAAGTAAGCTTCTGCAAGAACCACCTCCCTAAGGGTGATGAAGTTATGACACTGATCGATGAAGATGGCGACGAGTACCCGACCATATACTTGGCCCGAAAAACAGGACTCAGCGGCGGATGGAAAGGATTTGCAGTTGCTCATGATCTGGTGGATGGTGATGCATTGGTTTTTCAATTGATCCGGCCTACGACGTGTACATTATACGGGTGGAAAGACCCGGACAGGCAAGCTCAGAAGGATCAACTTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

236

Amino Acids

26.54

Weight (kDa)

9.35

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 135 - 216 2.5e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 232
AccII CGCG 2 cut(s) 153, 347
AciI CCGC 5 cut(s) 251, 342, 345, 567, 570
AclWI GGATC 4 cut(s) 378, 391, 628, 702
AcsI RAATTY 1 cut(s) 124
AfaI GTAC 3 cut(s) 29, 530, 652
AfiI CCNNNNNNNGG 2 cut(s) 291, 484
AflIII ACRYGT 2 cut(s) 424, 647
AgsI TTSAA 2 cut(s) 455, 629
AjiI CACGTC 1 cut(s) 648
AluBI AGCT 5 cut(s) 80, 107, 279, 462, 686
AluI AGCT 5 cut(s) 80, 107, 279, 462, 686
Alw26I GTCTC 1 cut(s) 42
AlwI GGATC 4 cut(s) 378, 391, 628, 702
Ama87I CYCGRG 1 cut(s) 207
AoxI GGCC 3 cut(s) 372, 546, 638
ApeKI GCWGC 2 cut(s) 46, 415
ApoI RAATTY 1 cut(s) 124
AspLEI GCGC 2 cut(s) 153, 155
AspS9I GGNCC 2 cut(s) 201, 547
AsuC2I CCSGG 3 cut(s) 157, 314, 674
AsuHPI GGTGA 6 cut(s) 16, 205, 364, 383, 500, 623
AvaI CYCGRG 1 cut(s) 207
AvaII GGWCC 1 cut(s) 201
AxyI CCTNAGG 1 cut(s) 483
BamHI GGATCC 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 443
BbvI GCAGC 2 cut(s) 58, 402
BccI CCATC 5 cut(s) 178, 343, 512, 567, 602
BceAI ACGGC 1 cut(s) 24
BcnI CCSGG 3 cut(s) 157, 314, 674
BcoDI GTCTC 1 cut(s) 42
BfuAI ACCTGC 1 cut(s) 232
BisI GCNGC 5 cut(s) 47, 342, 345, 416, 568
BlsI GCNGC 5 cut(s) 48, 343, 346, 417, 569
Bme1390I CCNGG 3 cut(s) 157, 314, 674
Bme18I GGWCC 1 cut(s) 201
BmeT110I CYCGRG 1 cut(s) 207
BmgBI CACGTC 1 cut(s) 648
BmgT120I GGNCC 2 cut(s) 201, 547
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 3 cut(s) 157, 314, 674
BmsI GCATC 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 443
BplI GAGNNNNNCTC 2 cut(s) 190, 222
BpuEI CTTGAG 1 cut(s) 87
BpuMI CCSGG 3 cut(s) 157, 314, 674
Bsa29I ATCGAT 1 cut(s) 510
BsaAI YACGTR 1 cut(s) 171
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 484
Bse1I ACTGG 2 cut(s) 375, 436
Bse21I CCTNAGG 1 cut(s) 483
BseCI ATCGAT 1 cut(s) 510
BseGI GGATG 3 cut(s) 307, 578, 613
BseLI CCNNNNNNNGG 2 cut(s) 291, 484
BseMII CTCAG 2 cut(s) 577, 701
BseNI ACTGG 2 cut(s) 375, 436
BsePI GCGCGC 1 cut(s) 151
BseRI GAGGAG 2 cut(s) 47, 211
BseXI GCAGC 2 cut(s) 58, 402
BseYI CCCAGC 1 cut(s) 445
BsgI GTGCAG 2 cut(s) 200, 210
Bsh1236I CGCG 2 cut(s) 153, 347
BshFI GGCC 3 cut(s) 374, 548, 640
BshVI ATCGAT 1 cut(s) 510
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 4 cut(s) 156, 313, 637, 674
BsiWI CGTACG 1 cut(s) 27
BslI CCNNNNNNNGG 2 cut(s) 291, 484
BsmAI GTCTC 1 cut(s) 42
BsnI GGCC 3 cut(s) 374, 548, 640
BsoBI CYCGRG 1 cut(s) 207
Bsp1407I TGTACA 1 cut(s) 650
Bsp143I GATC 6 cut(s) 383, 388, 507, 598, 633, 694
BspACI CCGC 5 cut(s) 251, 342, 345, 567, 570
BspANI GGCC 3 cut(s) 374, 548, 640
BspCNI CTCAG 2 cut(s) 576, 700
BspDI ATCGAT 1 cut(s) 510
BspFNI CGCG 2 cut(s) 153, 347
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 1 cut(s) 385
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 4 cut(s) 378, 391, 628, 702
BsrGI TGTACA 1 cut(s) 650
BsrI ACTGG 2 cut(s) 375, 436
BssHII GCGCGC 1 cut(s) 151
BssMI GATC 6 cut(s) 383, 388, 507, 598, 633, 694
BstAUI TGTACA 1 cut(s) 650
BstBAI YACGTR 1 cut(s) 171
BstC8I GCNNGC 4 cut(s) 44, 99, 153, 684
BstDEI CTNAG 4 cut(s) 483, 563, 687, 701
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 3 cut(s) 307, 578, 613
BstFNI CGCG 2 cut(s) 153, 347
BstHHI GCGC 2 cut(s) 153, 155
BstKTI GATC 6 cut(s) 386, 391, 510, 601, 636, 697
BstMAI GTCTC 1 cut(s) 42
BstMBI GATC 6 cut(s) 383, 388, 507, 598, 633, 694
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 3 cut(s) 155, 312, 672
BstUI CGCG 2 cut(s) 153, 347
BstV1I GCAGC 2 cut(s) 58, 402
BstV2I GAAGAC 1 cut(s) 443
BstX2I RGATCY 1 cut(s) 383
BstXI CCANNNNNNTGG 1 cut(s) 544
BstYI RGATCY 1 cut(s) 383
Bsu15I ATCGAT 1 cut(s) 510
Bsu36I CCTNAGG 1 cut(s) 483
BsuRI GGCC 3 cut(s) 374, 548, 640
BsuTUI ATCGAT 1 cut(s) 510
BtgZI GCGATG 1 cut(s) 362
BtrI CACGTC 1 cut(s) 648
BtsCI GGATG 3 cut(s) 307, 578, 613
BtsIMutI CAGTG 2 cut(s) 429, 503
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 4 cut(s) 44, 99, 153, 684
CciI TCATGA 1 cut(s) 595
CfoI GCGC 2 cut(s) 153, 155
Cfr13I GGNCC 2 cut(s) 201, 547
ClaI ATCGAT 1 cut(s) 510
Csp6I GTAC 3 cut(s) 28, 529, 651
CspCI CAANNNNNGTGG 2 cut(s) 193, 228
CviAII CATG 2 cut(s) 425, 596
CviQI GTAC 3 cut(s) 28, 529, 651
DdeI CTNAG 4 cut(s) 483, 563, 687, 701
DpnI GATC 6 cut(s) 385, 390, 509, 600, 635, 696
DpnII GATC 6 cut(s) 383, 388, 507, 598, 633, 694
DrdI GACNNNNNNGTC 1 cut(s) 13
DseDI GACNNNNNNGTC 1 cut(s) 13
EciI GGCGGA 2 cut(s) 266, 585
Eco47I GGWCC 1 cut(s) 201
Eco81I CCTNAGG 1 cut(s) 483
Eco88I CYCGRG 1 cut(s) 207
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoT22I ATGCAT 1 cut(s) 619
FaeI CATG 2 cut(s) 428, 599
FatI CATG 2 cut(s) 424, 595
Fnu4HI GCNGC 5 cut(s) 47, 342, 345, 416, 568
FokI GGATG 3 cut(s) 314, 585, 620
Fsp4HI GCNGC 5 cut(s) 47, 342, 345, 416, 568
GlaI GCGC 2 cut(s) 152, 154
GluI GCNGC 5 cut(s) 47, 342, 345, 416, 568
GsaI CCCAGC 1 cut(s) 449
HaeIII GGCC 3 cut(s) 374, 548, 640
HapII CCGG 4 cut(s) 156, 313, 637, 674
HhaI GCGC 2 cut(s) 153, 155
Hin1II CATG 2 cut(s) 428, 599
Hin6I GCGC 2 cut(s) 151, 153
HinP1I GCGC 2 cut(s) 151, 153
HindIII AAGCTT 1 cut(s) 460
HinfI GANTC 4 cut(s) 35, 265, 438, 561
HpaII CCGG 4 cut(s) 156, 313, 637, 674
HphI GGTGA 6 cut(s) 16, 205, 364, 383, 500, 623
Hpy166II GTNNAC 2 cut(s) 204, 651
Hpy188I TCNGA 4 cut(s) 270, 328, 388, 690
Hpy188III TCNNGA 1 cut(s) 596
Hpy8I GTNNAC 2 cut(s) 204, 651
Hpy99I CGWCG 3 cut(s) 365, 527, 649
HpyAV CCTTC 2 cut(s) 267, 685
HpyCH4IV ACGT 2 cut(s) 170, 647
HpyCH4V TGCA 7 cut(s) 181, 215, 227, 418, 468, 587, 617
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 4 cut(s) 483, 563, 687, 701
HpySE526I ACGT 2 cut(s) 170, 647
Hsp92II CATG 2 cut(s) 428, 599
HspAI GCGC 2 cut(s) 151, 153
Kzo9I GATC 6 cut(s) 383, 388, 507, 598, 633, 694
LmnI GCTCC 2 cut(s) 253, 276
Lsp1109I GCAGC 2 cut(s) 58, 402
LweI GCATC 1 cut(s) 604
MaeII ACGT 2 cut(s) 170, 647
MaeIII GTNAC 2 cut(s) 4, 427
MalI GATC 6 cut(s) 385, 390, 509, 600, 635, 696
MboI GATC 6 cut(s) 383, 388, 507, 598, 633, 694
MboII GAAGA 2 cut(s) 443, 527
MfeI CAATTG 1 cut(s) 629
MflI RGATCY 1 cut(s) 383
MluCI AATT 4 cut(s) 20, 124, 365, 629
MlyI GAGTC 2 cut(s) 44, 555
Mph1103I ATGCAT 1 cut(s) 619
MseI TTAA 1 cut(s) 111
MslI CAYNNNNRTG 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 567
MspI CCGG 4 cut(s) 156, 313, 637, 674
MspR9I CCNGG 3 cut(s) 157, 314, 674
MunI CAATTG 1 cut(s) 629
MvnI CGCG 2 cut(s) 153, 347
MwoI GCNNNNNNNGC 1 cut(s) 341
NciI CCSGG 3 cut(s) 157, 314, 674
NdeII GATC 6 cut(s) 383, 388, 507, 598, 633, 694
NlaIII CATG 2 cut(s) 428, 599
NlaIV GGNNCC 1 cut(s) 385
NmuCI GTSAC 2 cut(s) 4, 427
NsiI ATGCAT 1 cut(s) 619
NspI RCATGY 1 cut(s) 428
PagI TCATGA 1 cut(s) 595
PauI GCGCGC 1 cut(s) 151
PciI ACATGT 1 cut(s) 424
PcsI WCGNNNNNNNCGW 1 cut(s) 46
PfeI GAWTC 2 cut(s) 265, 438
Pfl23II CGTACG 1 cut(s) 27
PkrI GCNGC 5 cut(s) 48, 343, 346, 417, 569
PleI GAGTC 2 cut(s) 43, 555
PpsI GAGTC 2 cut(s) 43, 555
Ppu21I YACGTR 1 cut(s) 171
PscI ACATGT 1 cut(s) 424
PspFI CCCAGC 1 cut(s) 445
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 385
PspPI GGNCC 2 cut(s) 201, 547
PsuI RGATCY 1 cut(s) 383
PteI GCGCGC 1 cut(s) 151
RsaI GTAC 3 cut(s) 29, 530, 652
RsaNI GTAC 3 cut(s) 28, 529, 651
RseI CAYNNNNRTG 1 cut(s) 300
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 5 cut(s) 47, 342, 345, 416, 568
Sau3AI GATC 6 cut(s) 383, 388, 507, 598, 633, 694
Sau96I GGNCC 2 cut(s) 201, 547
SchI GAGTC 2 cut(s) 44, 555
ScrFI CCNGG 3 cut(s) 157, 314, 674
SfaNI GCATC 1 cut(s) 604
SinI GGWCC 1 cut(s) 201
SmiMI CAYNNNNRTG 1 cut(s) 300
SmlI CTYRAG 1 cut(s) 102
SmoI CTYRAG 1 cut(s) 102
Sse9I AATT 4 cut(s) 20, 124, 365, 629
SsiI CCGC 5 cut(s) 251, 342, 345, 567, 570
StyD4I CCNGG 3 cut(s) 155, 312, 672
TaiI ACGT 2 cut(s) 173, 650
TaqI TCGA 2 cut(s) 220, 510
TasI AATT 4 cut(s) 20, 124, 365, 629
TatI WGTACW 1 cut(s) 650
TauI GCSGC 3 cut(s) 344, 347, 570
TfiI GAWTC 2 cut(s) 265, 438
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 2 cut(s) 436, 510
TseFI GTSAC 2 cut(s) 4, 427
TseI GCWGC 2 cut(s) 46, 415
Tsp45I GTSAC 2 cut(s) 4, 427
TspDTI ATGAA 4 cut(s) 161, 345, 507, 528
TspRI CASTG 2 cut(s) 436, 510
VpaK11BI GGWCC 1 cut(s) 201
XagI CCTNNNNNAGG 1 cut(s) 482
XapI RAATTY 1 cut(s) 124
XceI RCATGY 1 cut(s) 428
Zsp2I ATGCAT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.