MD08G1102800.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002837
Physical Location & Seq
Reverse (-)
8724390 .. 8727196
2807 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1102800.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGTGATGGCCAAGTCTAAGTTGTCTTACGAGGAGTCTCGCCGGCAGAGAATGGAGGAAAACAAGAAGAGAATGGAAGCTCTCAATCTCCCTCTGCTTGCTCAAGCTCTTAAAAAAACCCCAAATTCTCCCAAACCTTCCCCTATGAAGCGCGGGGGGAAACCTCGCACGTACGAGGTGCAGATGGTGGAGGTGAGGAGGTCCACCCGGGTTGCAAATCGACCTGCACCCGATTACAAAGAAGTTGTGGCGGAGCGAGTAATGATTCCAAGAAGGTGCTACGCCACCAATCGGCATAGGGATATGGCGAACCGGGTGTATGCTTCCGACGAAGCCAGAGCCGCCGCGATGGTGAAAGCCGACGAATTGGAGCTCAGTTTGGGATCCGATCACCCGACATTTGTGAAGACAATGCTCCAATCACATGTCACTGGTGGATTCTGGCTGGGTCTTCAAGTCAGCTTCTGCAAGAACCACCTCCCAAAGGGTGAAGAAATTATGACATTGATCGATGAAGTTGGAGCCGAGTACCCGACTATATACTTGGCCCGGAAAACTGGACTCAGCGGCGGATGGAAAGGATTTGCAGTTGCTCATGATCTTGTGGATGGAGATGCATTGGTTTTTCAATTGATACGGCCAACAACATTCAAGGTGTACATTATAAGGGTGGAGAGACCTGGGCAGGCAAGCTCAGAAGAATCAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

237

Amino Acids

26.65

Weight (kDa)

9.62

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 134 - 223 3.7e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 665
Acc36I ACCTGC 1 cut(s) 232
AccII CGCG 2 cut(s) 153, 347
AciI CCGC 6 cut(s) 153, 251, 342, 345, 567, 570
AclWI GGATC 2 cut(s) 378, 391
AcoI YGGCCR 2 cut(s) 9, 638
AcsI RAATTY 1 cut(s) 124
AfaI GTAC 3 cut(s) 173, 530, 659
AfiI CCNNNNNNNGG 2 cut(s) 291, 484
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 3 cut(s) 455, 629, 652
AjnI CCWGG 1 cut(s) 679
AluBI AGCT 5 cut(s) 80, 107, 373, 462, 693
AluI AGCT 5 cut(s) 80, 107, 373, 462, 693
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 2 cut(s) 42, 670
AlwI GGATC 2 cut(s) 378, 391
AlwNI CAGNNNCTG 1 cut(s) 465
Ama87I CYCGRG 1 cut(s) 207
AoxI GGCC 3 cut(s) 9, 546, 638
ApoI RAATTY 1 cut(s) 124
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 2 cut(s) 201, 547
AsuC2I CCSGG 4 cut(s) 208, 209, 314, 550
AsuHPI GGTGA 5 cut(s) 16, 205, 364, 383, 500
AvaI CYCGRG 1 cut(s) 207
AvaII GGWCC 1 cut(s) 201
BalI TGGCCA 1 cut(s) 11
BamHI GGATCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 375
BbsI GAAGAC 2 cut(s) 413, 443
Bbv12I GWGCWC 1 cut(s) 375
BccI CCATC 4 cut(s) 178, 343, 567, 602
BceAI ACGGC 1 cut(s) 653
BciT130I CCWGG 1 cut(s) 681
BcnI CCSGG 4 cut(s) 208, 209, 314, 550
BcoDI GTCTC 2 cut(s) 42, 670
BfuAI ACCTGC 1 cut(s) 232
BisI GCNGC 3 cut(s) 342, 345, 568
BlsI GCNGC 3 cut(s) 343, 346, 569
Bme1390I CCNGG 5 cut(s) 208, 209, 314, 550, 681
Bme18I GGWCC 1 cut(s) 201
BmeT110I CYCGRG 1 cut(s) 207
BmgT120I GGNCC 2 cut(s) 201, 547
BmiI GGNNCC 2 cut(s) 385, 523
BmrFI CCNGG 5 cut(s) 208, 209, 314, 550, 681
BmsI GCATC 1 cut(s) 604
BpiI GAAGAC 2 cut(s) 413, 443
BpuEI CTTGAG 1 cut(s) 87
BpuMI CCSGG 4 cut(s) 208, 209, 314, 550
Bsa29I ATCGAT 1 cut(s) 510
BsaAI YACGTR 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 670
BsaJI CCNNGG 2 cut(s) 207, 680
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 484
Bse118I RCCGGY 1 cut(s) 42
Bse1I ACTGG 2 cut(s) 436, 562
BseBI CCWGG 1 cut(s) 681
BseCI ATCGAT 1 cut(s) 510
BseDI CCNNGG 2 cut(s) 207, 680
BseGI GGATG 2 cut(s) 578, 613
BseLI CCNNNNNNNGG 2 cut(s) 291, 484
BseMII CTCAG 3 cut(s) 388, 577, 708
BseNI ACTGG 2 cut(s) 436, 562
BseRI GAGGAG 2 cut(s) 47, 211
BseYI CCCAGC 1 cut(s) 445
BsgI GTGCAG 2 cut(s) 200, 210
Bsh1236I CGCG 2 cut(s) 153, 347
BshFI GGCC 3 cut(s) 11, 548, 640
BshVI ATCGAT 1 cut(s) 510
BsiHKAI GWGCWC 1 cut(s) 375
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 4 cut(s) 43, 208, 313, 550
BsiWI CGTACG 1 cut(s) 171
BslI CCNNNNNNNGG 2 cut(s) 291, 484
BsmAI GTCTC 2 cut(s) 42, 670
BsnI GGCC 3 cut(s) 11, 548, 640
Bso31I GGTCTC 1 cut(s) 670
BsoBI CYCGRG 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 375
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 4 cut(s) 383, 388, 507, 598
BspACI CCGC 6 cut(s) 153, 251, 342, 345, 567, 570
BspANI GGCC 3 cut(s) 11, 548, 640
BspCNI CTCAG 3 cut(s) 387, 576, 707
BspDI ATCGAT 1 cut(s) 510
BspFNI CGCG 2 cut(s) 153, 347
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 2 cut(s) 385, 523
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 2 cut(s) 378, 391
BspTNI GGTCTC 1 cut(s) 670
BsrFI RCCGGY 1 cut(s) 42
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 2 cut(s) 436, 562
BssAI RCCGGY 1 cut(s) 42
BssECI CCNNGG 2 cut(s) 207, 680
BssMI GATC 4 cut(s) 383, 388, 507, 598
Bst2UI CCWGG 1 cut(s) 681
Bst6I CTCTTC 1 cut(s) 62
BstAUI TGTACA 1 cut(s) 657
BstBAI YACGTR 1 cut(s) 171
BstC8I GCNNGC 4 cut(s) 44, 99, 687, 691
BstDEI CTNAG 5 cut(s) 18, 374, 563, 694, 708
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 2 cut(s) 578, 613
BstFNI CGCG 2 cut(s) 153, 347
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 4 cut(s) 386, 391, 510, 601
BstMAI GTCTC 2 cut(s) 42, 670
BstMBI GATC 4 cut(s) 383, 388, 507, 598
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstNI CCWGG 1 cut(s) 681
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 5 cut(s) 206, 207, 312, 548, 679
BstUI CGCG 2 cut(s) 153, 347
BstV2I GAAGAC 2 cut(s) 413, 443
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
Bsu15I ATCGAT 1 cut(s) 510
BsuRI GGCC 3 cut(s) 11, 548, 640
BsuTUI ATCGAT 1 cut(s) 510
BtgZI GCGATG 1 cut(s) 362
BtsCI GGATG 2 cut(s) 578, 613
BtsIMutI CAGTG 1 cut(s) 429
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 4 cut(s) 44, 99, 687, 691
CaiI CAGNNNCTG 1 cut(s) 465
CciI TCATGA 1 cut(s) 595
CfoI GCGC 1 cut(s) 153
Cfr10I RCCGGY 1 cut(s) 42
Cfr13I GGNCC 2 cut(s) 201, 547
Cfr9I CCCGGG 1 cut(s) 207
ClaI ATCGAT 1 cut(s) 510
Csp6I GTAC 3 cut(s) 172, 529, 658
CspCI CAANNNNNGTGG 2 cut(s) 193, 228
CviAII CATG 2 cut(s) 425, 596
CviQI GTAC 3 cut(s) 172, 529, 658
DdeI CTNAG 5 cut(s) 18, 374, 563, 694, 708
DpnI GATC 4 cut(s) 385, 390, 509, 600
DpnII GATC 4 cut(s) 383, 388, 507, 598
EaeI YGGCCR 2 cut(s) 9, 638
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
EciI GGCGGA 2 cut(s) 266, 585
Ecl136II GAGCTC 1 cut(s) 373
Eco24I GRGCYC 1 cut(s) 375
Eco31I GGTCTC 1 cut(s) 670
Eco47I GGWCC 1 cut(s) 201
Eco53kI GAGCTC 1 cut(s) 373
Eco88I CYCGRG 1 cut(s) 207
EcoICRI GAGCTC 1 cut(s) 373
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoRII CCWGG 1 cut(s) 679
EcoT22I ATGCAT 1 cut(s) 619
EcoT38I GRGCYC 1 cut(s) 375
FaeI CATG 2 cut(s) 428, 599
FatI CATG 2 cut(s) 424, 595
FauI CCCGC 1 cut(s) 146
Fnu4HI GCNGC 3 cut(s) 342, 345, 568
FokI GGATG 2 cut(s) 585, 620
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 3 cut(s) 342, 345, 568
GlaI GCGC 1 cut(s) 152
GluI GCNGC 3 cut(s) 342, 345, 568
GsaI CCCAGC 1 cut(s) 449
HaeIII GGCC 3 cut(s) 11, 548, 640
HapII CCGG 4 cut(s) 43, 208, 313, 550
HhaI GCGC 1 cut(s) 153
Hin1II CATG 2 cut(s) 428, 599
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HinfI GANTC 5 cut(s) 35, 265, 438, 561, 701
HpaII CCGG 4 cut(s) 43, 208, 313, 550
HphI GGTGA 5 cut(s) 16, 205, 364, 383, 500
Hpy166II GTNNAC 2 cut(s) 204, 658
Hpy188I TCNGA 3 cut(s) 328, 388, 697
Hpy188III TCNNGA 1 cut(s) 596
Hpy8I GTNNAC 2 cut(s) 204, 658
Hpy99I CGWCG 2 cut(s) 332, 365
HpyAV CCTTC 2 cut(s) 147, 267
HpyCH4IV ACGT 1 cut(s) 170
HpyCH4V TGCA 6 cut(s) 181, 215, 227, 468, 587, 617
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 5 cut(s) 18, 374, 563, 694, 708
HpySE526I ACGT 1 cut(s) 170
Hsp92II CATG 2 cut(s) 428, 599
HspAI GCGC 1 cut(s) 151
KroI GCCGGC 1 cut(s) 42
KroNI GCCGGC 1 cut(s) 44
Kzo9I GATC 4 cut(s) 383, 388, 507, 598
LmnI GCTCC 4 cut(s) 253, 370, 420, 521
LweI GCATC 1 cut(s) 604
MaeII ACGT 1 cut(s) 170
MaeIII GTNAC 1 cut(s) 427
MalI GATC 4 cut(s) 385, 390, 509, 600
MboI GATC 4 cut(s) 383, 388, 507, 598
MboII GAAGA 5 cut(s) 79, 418, 443, 503, 710
MfeI CAATTG 1 cut(s) 629
MflI RGATCY 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 375
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 4 cut(s) 124, 365, 495, 629
MluNI TGGCCA 1 cut(s) 11
MlyI GAGTC 2 cut(s) 44, 555
MmeI TCCRAC 2 cut(s) 351, 499
MnlI CCTC 9 cut(s) 25, 49, 102, 169, 174, 184, 189, 192, 488
Mox20I TGGCCA 1 cut(s) 11
Mph1103I ATGCAT 1 cut(s) 619
MroNI GCCGGC 1 cut(s) 42
MscI TGGCCA 1 cut(s) 11
MseI TTAA 1 cut(s) 111
Msp20I TGGCCA 1 cut(s) 11
MspA1I CMGCKG 1 cut(s) 567
MspI CCGG 4 cut(s) 43, 208, 313, 550
MspR9I CCNGG 5 cut(s) 208, 209, 314, 550, 681
MunI CAATTG 1 cut(s) 629
MvaI CCWGG 1 cut(s) 681
MvnI CGCG 2 cut(s) 153, 347
MwoI GCNNNNNNNGC 1 cut(s) 341
NaeI GCCGGC 1 cut(s) 44
NciI CCSGG 4 cut(s) 208, 209, 314, 550
NdeII GATC 4 cut(s) 383, 388, 507, 598
NgoMIV GCCGGC 1 cut(s) 42
NlaIII CATG 2 cut(s) 428, 599
NlaIV GGNNCC 2 cut(s) 385, 523
NmeAIII GCCGAG 1 cut(s) 550
NmuCI GTSAC 1 cut(s) 427
NsiI ATGCAT 1 cut(s) 619
NspI RCATGY 1 cut(s) 428
PagI TCATGA 1 cut(s) 595
PciI ACATGT 1 cut(s) 424
PdiI GCCGGC 1 cut(s) 44
PfeI GAWTC 3 cut(s) 265, 438, 701
Pfl23II CGTACG 1 cut(s) 171
PkrI GCNGC 3 cut(s) 343, 346, 569
PleI GAGTC 2 cut(s) 43, 555
PpsI GAGTC 2 cut(s) 43, 555
Ppu21I YACGTR 1 cut(s) 171
PscI ACATGT 1 cut(s) 424
PsiI TTATAA 1 cut(s) 665
Psp124BI GAGCTC 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 679
PspFI CCCAGC 1 cut(s) 445
PspGI CCWGG 1 cut(s) 679
PspLI CGTACG 1 cut(s) 171
PspN4I GGNNCC 2 cut(s) 385, 523
PspPI GGNCC 2 cut(s) 201, 547
PstNI CAGNNNCTG 1 cut(s) 465
PsuI RGATCY 1 cut(s) 383
RsaI GTAC 3 cut(s) 173, 530, 659
RsaNI GTAC 3 cut(s) 172, 529, 658
SacI GAGCTC 1 cut(s) 375
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 3 cut(s) 342, 345, 568
Sau3AI GATC 4 cut(s) 383, 388, 507, 598
Sau96I GGNCC 2 cut(s) 201, 547
SchI GAGTC 2 cut(s) 44, 555
ScrFI CCNGG 5 cut(s) 208, 209, 314, 550, 681
SduI GDGCHC 1 cut(s) 375
SfaNI GCATC 1 cut(s) 604
SinI GGWCC 1 cut(s) 201
SmaI CCCGGG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 102
SmoI CTYRAG 1 cut(s) 102
Sse9I AATT 4 cut(s) 124, 365, 495, 629
SsiI CCGC 6 cut(s) 153, 251, 342, 345, 567, 570
SstI GAGCTC 1 cut(s) 375
StyD4I CCNGG 5 cut(s) 206, 207, 312, 548, 679
TaiI ACGT 1 cut(s) 173
TaqI TCGA 2 cut(s) 220, 510
TasI AATT 4 cut(s) 124, 365, 495, 629
TatI WGTACW 1 cut(s) 657
TauI GCSGC 3 cut(s) 344, 347, 570
TfiI GAWTC 3 cut(s) 265, 438, 701
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 1 cut(s) 427
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 2 cut(s) 161, 528
TspMI CCCGGG 1 cut(s) 207
TspRI CASTG 1 cut(s) 436
VpaK11BI GGWCC 1 cut(s) 201
XagI CCTNNNNNAGG 1 cut(s) 482
XapI RAATTY 1 cut(s) 124
XceI RCATGY 1 cut(s) 428
XmaI CCCGGG 1 cut(s) 207
Zsp2I ATGCAT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.