Rorug06G0360000

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002837
Physical Location & Seq
Reverse (-)
50859339 .. 50863400
4062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0360000.1

Sequence Viewer

Length: 924 bp
ATGCCAAAACAACATCCAAATCAGAGTCAGTGTATGCAGGCGTTGGGAATAAAAAAAAAATACAAACCTGTGGAAAAAAATGAAGGCCTCCAATTTATATTGGTGGATGAAATGAATGATGCAATCCACGGATTCATAAGACAAAGGAACTGCGAACTACTGGCATCCAACATTGTCCAAGGAAATATATATGAAATCAGCCGCTTCTTTACAAGCTACTTCAACTACTTTCATATTGCAGACAAAATTGCACTATCAAACACAGGGAACACTTTCTTTTTCCTGAATCCGGATATGAAGGAGGCTGAGGACTACAAACAATACTTTGGCGAACCTCATCATGCTACACACATCTTATCAACCCGACCCAAACAACCAAGGACTCCGGCAGAGTTACAGGCTGTCAAACAGATGTCTGTCTCAGATTTAAACATCTGTGATCCAAACAACTATAAGAATAAAACTGTATATTGCACAGCGAAAATAGGCAAATTTGTGCCAGAAGAAAGGTGGTTCTATTTGGCTTGCCCAAAATGCTTCAAGGAAATGAGGAAAAAGCCACAAAGCCAATTACTAATCTGTGCAGAACATGATATGCAGCAACCTCTTTACAGATTCATAGTAACTGCATTGATAAATGATGAGACTGATGAGGCATCAGCCATCATAAAGGGGAAACAGGCGGAAGAACTATTTGGTACGACATGTGAGGACCTCATCCTCAACAAAAATCTTGACAATCCAAATATACTACCACCAGAAATTCTCCGAATAGAGGGCCAAATCAAACTCCTGGAACTTGAAATAGGCACTGCACAAAACGTGATTGTCAAGGGAATCCATGAAGATATTCGTGGACTGAATCCAACACAGAGAATTCAGCCCTCGAGCTCAACAGCCGACAACATCCAGCCCTCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

307

Amino Acids

35.14

Weight (kDa)

6.46

Isoelectric Point (pI)

54.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep_fac-A_C PF08646 164 - 244 4.8e-08 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 289
AciI CCGC 2 cut(s) 202, 683
AclWI GGATC 1 cut(s) 434
AcsI RAATTY 3 cut(s) 491, 762, 876
AfaI GTAC 1 cut(s) 700
AfiI CCNNNNNNNGG 2 cut(s) 289, 775
AflIII ACRYGT 1 cut(s) 704
AgsI TTSAA 3 cut(s) 223, 541, 803
AjnI CCWGG 1 cut(s) 792
AjuI GAANNNNNNNTTGG 2 cut(s) 678, 710
AluBI AGCT 3 cut(s) 216, 891, 921
AluI AGCT 3 cut(s) 216, 891, 921
Alw21I GWGCWC 1 cut(s) 893
Alw26I GTCTC 2 cut(s) 424, 638
AlwI GGATC 1 cut(s) 434
Ama87I CYCGRG 1 cut(s) 886
Aor13HI TCCGGA 1 cut(s) 289
AoxI GGCC 2 cut(s) 85, 778
ApeKI GCWGC 1 cut(s) 598
ApoI RAATTY 3 cut(s) 491, 762, 876
Asp700I GAANNNNTTC 2 cut(s) 272, 849
AspS9I GGNCC 2 cut(s) 712, 778
AvaI CYCGRG 1 cut(s) 886
AvaII GGWCC 1 cut(s) 712
BanII GRGCYC 1 cut(s) 893
Bbv12I GWGCWC 1 cut(s) 893
BbvCI CCTCAGC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 610
BccI CCATC 1 cut(s) 671
BciT130I CCWGG 1 cut(s) 794
BcoDI GTCTC 2 cut(s) 424, 638
BisI GCNGC 2 cut(s) 202, 599
BlsI GCNGC 2 cut(s) 203, 600
Bme1390I CCNGG 1 cut(s) 794
Bme18I GGWCC 1 cut(s) 712
BmeT110I CYCGRG 1 cut(s) 886
BmgT120I GGNCC 2 cut(s) 712, 778
BmrFI CCNGG 1 cut(s) 794
BmsI GCATC 3 cut(s) 109, 173, 665
Bpu10I CCTNAGC 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 901
BsaJI CCNNGG 3 cut(s) 127, 178, 377
BsaWI WCCGGW 1 cut(s) 289
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 775
Bse1I ACTGG 1 cut(s) 165
BseAI TCCGGA 1 cut(s) 289
BseBI CCWGG 1 cut(s) 794
BseDI CCNNGG 3 cut(s) 127, 178, 377
BseGI GGATG 5 cut(s) 13, 112, 164, 717, 906
BseLI CCNNNNNNNGG 2 cut(s) 289, 775
BseMII CTCAG 2 cut(s) 297, 435
BseNI ACTGG 1 cut(s) 165
BseXI GCAGC 1 cut(s) 610
BsgI GTGCAG 2 cut(s) 603, 798
BshFI GGCC 2 cut(s) 87, 780
BsiHKAI GWGCWC 1 cut(s) 893
BsiHKCI CYCGRG 1 cut(s) 886
BsiSI CCGG 2 cut(s) 290, 386
BslI CCNNNNNNNGG 2 cut(s) 289, 775
BsmAI GTCTC 2 cut(s) 424, 638
BsnI GGCC 2 cut(s) 87, 780
BsoBI CYCGRG 1 cut(s) 886
Bsp1286I GDGCHC 1 cut(s) 893
Bsp13I TCCGGA 1 cut(s) 289
Bsp143I GATC 1 cut(s) 439
BspACI CCGC 2 cut(s) 202, 683
BspANI GGCC 2 cut(s) 87, 780
BspCNI CTCAG 2 cut(s) 298, 434
BspEI TCCGGA 1 cut(s) 289
BspPI GGATC 1 cut(s) 434
BsrI ACTGG 1 cut(s) 165
BssECI CCNNGG 3 cut(s) 127, 178, 377
BssMI GATC 1 cut(s) 439
BssT1I CCWWGG 2 cut(s) 178, 377
Bst2UI CCWGG 1 cut(s) 794
Bst4CI ACNGT 1 cut(s) 466
BstC8I GCNNGC 2 cut(s) 39, 526
BstDEI CTNAG 2 cut(s) 306, 421
BstDSI CCRYGG 1 cut(s) 127
BstF5I GGATG 5 cut(s) 13, 112, 164, 717, 906
BstKTI GATC 1 cut(s) 442
BstMAI GTCTC 2 cut(s) 424, 638
BstMBI GATC 1 cut(s) 439
BstMWI GCNNNNNNNGC 1 cut(s) 534
BstNI CCWGG 1 cut(s) 794
BstNSI RCATGY 1 cut(s) 708
BstSCI CCNGG 1 cut(s) 792
BstV1I GCAGC 1 cut(s) 610
BsuRI GGCC 2 cut(s) 87, 780
BtgI CCRYGG 1 cut(s) 127
BtsCI GGATG 5 cut(s) 13, 112, 164, 717, 906
BtsI GCAGTG 1 cut(s) 810
BtsIMutI CAGTG 2 cut(s) 35, 810
Cac8I GCNNGC 2 cut(s) 39, 526
Cfr13I GGNCC 2 cut(s) 712, 778
Csp6I GTAC 1 cut(s) 699
CviAII CATG 4 cut(s) 341, 590, 705, 842
CviQI GTAC 1 cut(s) 699
DdeI CTNAG 2 cut(s) 306, 421
DpnI GATC 1 cut(s) 441
DpnII GATC 1 cut(s) 439
DraI TTTAAA 1 cut(s) 429
EciI GGCGGA 1 cut(s) 698
Ecl136II GAGCTC 1 cut(s) 891
Eco130I CCWWGG 2 cut(s) 178, 377
Eco147I AGGCCT 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 893
Eco47I GGWCC 1 cut(s) 712
Eco53kI GAGCTC 1 cut(s) 891
Eco88I CYCGRG 1 cut(s) 886
EcoICRI GAGCTC 1 cut(s) 891
EcoO109I RGGNCCY 1 cut(s) 712
EcoRI GAATTC 1 cut(s) 876
EcoRII CCWGG 1 cut(s) 792
EcoT14I CCWWGG 2 cut(s) 178, 377
EcoT38I GRGCYC 1 cut(s) 893
ErhI CCWWGG 2 cut(s) 178, 377
FaeI CATG 4 cut(s) 344, 593, 708, 845
FatI CATG 4 cut(s) 340, 589, 704, 841
Fnu4HI GCNGC 2 cut(s) 202, 599
FokI GGATG 4 cut(s) 119, 151, 704, 893
FriOI GRGCYC 1 cut(s) 893
Fsp4HI GCNGC 2 cut(s) 202, 599
GluI GCNGC 2 cut(s) 202, 599
HaeIII GGCC 2 cut(s) 87, 780
HapII CCGG 2 cut(s) 290, 386
Hin1II CATG 4 cut(s) 344, 593, 708, 845
HinfI GANTC 7 cut(s) 25, 132, 286, 382, 615, 837, 862
HpaII CCGG 2 cut(s) 290, 386
Hpy166II GTNNAC 1 cut(s) 857
Hpy188I TCNGA 3 cut(s) 24, 424, 770
Hpy188III TCNNGA 3 cut(s) 283, 290, 734
Hpy8I GTNNAC 1 cut(s) 857
HpyAV CCTTC 2 cut(s) 77, 292
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4IV ACGT 1 cut(s) 822
HpyCH4V TGCA 9 cut(s) 37, 122, 239, 251, 474, 584, 598, 629, 815
HpyF10VI GCNNNNNNNGC 1 cut(s) 534
HpyF3I CTNAG 2 cut(s) 306, 421
HpySE526I ACGT 1 cut(s) 822
Hsp92II CATG 4 cut(s) 344, 593, 708, 845
Kpn2I TCCGGA 1 cut(s) 289
Kzo9I GATC 1 cut(s) 439
Lsp1109I GCAGC 1 cut(s) 610
LweI GCATC 3 cut(s) 109, 173, 665
MaeII ACGT 1 cut(s) 822
MaeIII GTNAC 2 cut(s) 393, 622
MalI GATC 1 cut(s) 441
MboI GATC 1 cut(s) 439
MboII GAAGA 3 cut(s) 515, 698, 857
MhlI GDGCHC 1 cut(s) 893
MluCI AATT 6 cut(s) 92, 246, 491, 569, 762, 876
MlyI GAGTC 2 cut(s) 34, 376
MmeI TCCRAC 2 cut(s) 192, 890
MroI TCCGGA 1 cut(s) 289
MroXI GAANNNNTTC 2 cut(s) 272, 849
MseI TTAA 1 cut(s) 428
MspI CCGG 2 cut(s) 290, 386
MspR9I CCNGG 1 cut(s) 794
MvaI CCWGG 1 cut(s) 794
MwoI GCNNNNNNNGC 1 cut(s) 534
NdeII GATC 1 cut(s) 439
NlaIII CATG 4 cut(s) 344, 593, 708, 845
NspI RCATGY 1 cut(s) 708
PaeR7I CTCGAG 1 cut(s) 886
PceI AGGCCT 1 cut(s) 87
PciI ACATGT 1 cut(s) 704
PdmI GAANNNNTTC 2 cut(s) 272, 849
PfeI GAWTC 5 cut(s) 132, 286, 615, 837, 862
PfoI TCCNGGA 1 cut(s) 792
PkrI GCNGC 2 cut(s) 203, 600
PleI GAGTC 2 cut(s) 33, 376
PpsI GAGTC 2 cut(s) 33, 376
PpuMI RGGWCCY 1 cut(s) 712
PscI ACATGT 1 cut(s) 704
Psp124BI GAGCTC 1 cut(s) 893
Psp5II RGGWCCY 1 cut(s) 712
Psp6I CCWGG 1 cut(s) 792
PspGI CCWGG 1 cut(s) 792
PspPI GGNCC 2 cut(s) 712, 778
PspPPI RGGWCCY 1 cut(s) 712
PspXI VCTCGAGB 1 cut(s) 886
RsaI GTAC 1 cut(s) 700
RsaNI GTAC 1 cut(s) 699
SacI GAGCTC 1 cut(s) 893
SaqAI TTAA 1 cut(s) 428
SatI GCNGC 2 cut(s) 202, 599
Sau3AI GATC 1 cut(s) 439
Sau96I GGNCC 2 cut(s) 712, 778
SchI GAGTC 2 cut(s) 34, 376
ScrFI CCNGG 1 cut(s) 794
SduI GDGCHC 1 cut(s) 893
SetI ASST 9 cut(s) 70, 218, 337, 512, 607, 717, 825, 893, 923
SfaNI GCATC 3 cut(s) 109, 173, 665
Sfr274I CTCGAG 1 cut(s) 886
SinI GGWCC 1 cut(s) 712
SlaI CTCGAG 1 cut(s) 886
SmlI CTYRAG 2 cut(s) 886, 916
SmoI CTYRAG 2 cut(s) 886, 916
Sse9I AATT 6 cut(s) 92, 246, 491, 569, 762, 876
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 2 cut(s) 202, 683
SstI GAGCTC 1 cut(s) 893
StuI AGGCCT 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 792
StyI CCWWGG 2 cut(s) 178, 377
TaaI ACNGT 1 cut(s) 466
TaiI ACGT 1 cut(s) 825
TaqI TCGA 1 cut(s) 887
TasI AATT 6 cut(s) 92, 246, 491, 569, 762, 876
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 5 cut(s) 132, 286, 615, 837, 862
Tru1I TTAA 1 cut(s) 428
Tru9I TTAA 1 cut(s) 428
TscAI CASTG 2 cut(s) 35, 817
TseI GCWGC 1 cut(s) 598
TspDTI ATGAA 9 cut(s) 96, 123, 124, 128, 207, 221, 311, 607, 858
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 2 cut(s) 35, 817
VpaK11BI GGWCC 1 cut(s) 712
XapI RAATTY 3 cut(s) 491, 762, 876
XceI RCATGY 1 cut(s) 708
XcmI CCANNNNNNNNNTGG 1 cut(s) 507
XhoI CTCGAG 1 cut(s) 886
XmnI GAANNNNTTC 2 cut(s) 272, 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.