MD08G1102900.v1.1

B3 domain-containing protein At3g19184-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002837
Physical Location & Seq
Reverse (-)
8727816 .. 8730241
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1102900.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGTGGCGCCCAAGTTATCGTACGAGGAGTCTCGCCGGCAGCGAATGGAGGAAAACAAAAAGAGAATGGAAGCTCTCAATCTGCCTATGCTTGCTCAAGCTCTTAAGAAAACCCCAAATTCCCCAAAACCCTCCCCTATGAAGCGCGCGAAGGCCCGCACGGTGGAGAAGCAGCTCGTGGAGGTGAGGAGGTCCTCCCGGGTCGCAAATCTTCCGACCCCGGTTTACAAAGAAGTGGCTGTGGTGGATCGGGAGATGAGGCCCAGAAGGTATTCTACTAATAGACATAGGGATTTGTCAAACCGGGTTTATGCTTCCGATGAAGCCAGAGATGACGCCACTGCGAGAGCTGAGGCATTTGAGTCCGATTTGGGATCCGATTACCCGACCATGGTGAAACCGATGCTGCAATCCCATGTCACTGGTGGATTCTGGCTGGGTCTTCCGAAATATTTCTGCAGTAAGAACCTCCCGAAGCGTGATGATATTGTGATTTTGATAGATGAAGATGGGAACGAGTGCGAGGTTATATACTTGGCTGAGAAAAGAGGACTCAGTGGTGGATGGAGAGGGTTTGCAATTGATCATGATTTGGTGGATGGGGATGCATTGGTCTTTCAACTAATTCGGCCTAAAACGCTCAAGGTGTACATTTTAAGGGTGGAAAGAGCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

226

Amino Acids

25.82

Weight (kDa)

9.45

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 132 - 220 5.9e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 7
AccII CGCG 2 cut(s) 147, 149
AciI CCGC 1 cut(s) 157
AclWI GGATC 3 cut(s) 255, 369, 382
AcsI RAATTY 1 cut(s) 118
AcyI GRCGYC 2 cut(s) 8, 336
AfaI GTAC 2 cut(s) 23, 650
AfiI CCNNNNNNNGG 2 cut(s) 163, 391
AflII CTTAAG 1 cut(s) 104
AgsI TTSAA 1 cut(s) 620
AluBI AGCT 5 cut(s) 74, 101, 175, 350, 671
AluI AGCT 5 cut(s) 74, 101, 175, 350, 671
Alw26I GTCTC 1 cut(s) 36
AlwI GGATC 3 cut(s) 255, 369, 382
Ama87I CYCGRG 1 cut(s) 198
AoxI GGCC 3 cut(s) 153, 260, 629
ApeKI GCWGC 3 cut(s) 40, 172, 406
ApoI RAATTY 1 cut(s) 118
Asp700I GAANNNNTTC 2 cut(s) 271, 452
AspLEI GCGC 3 cut(s) 10, 147, 149
AspS9I GGNCC 3 cut(s) 154, 192, 261
AsuC2I CCSGG 4 cut(s) 199, 200, 221, 305
AsuHPI GGTGA 2 cut(s) 196, 406
AvaI CYCGRG 1 cut(s) 198
AvaII GGWCC 1 cut(s) 192
BamHI GGATCC 1 cut(s) 374
BanI GGYRCC 1 cut(s) 7
BarI GAAGNNNNNNTAC 2 cut(s) 259, 291
BauI CACGAG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 434
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 3 cut(s) 52, 184, 393
BccI CCATC 3 cut(s) 503, 558, 593
BclI TGATCA 1 cut(s) 583
BcnI CCSGG 4 cut(s) 199, 200, 221, 305
BcoDI GTCTC 1 cut(s) 36
BfmI CTRYAG 1 cut(s) 457
BfoI RGCGCY 1 cut(s) 11
BfrI CTTAAG 1 cut(s) 104
BisI GCNGC 3 cut(s) 41, 173, 407
BlsI GCNGC 3 cut(s) 42, 174, 408
Bme1390I CCNGG 4 cut(s) 199, 200, 221, 305
Bme18I GGWCC 1 cut(s) 192
BmeT110I CYCGRG 1 cut(s) 198
BmgT120I GGNCC 3 cut(s) 154, 192, 261
BmiI GGNNCC 2 cut(s) 9, 376
BmrFI CCNGG 4 cut(s) 199, 200, 221, 305
BmsI GCATC 2 cut(s) 393, 595
BpiI GAAGAC 1 cut(s) 434
Bpu10I CCTNAGC 1 cut(s) 351
BpuEI CTTGAG 2 cut(s) 81, 626
BpuMI CCSGG 4 cut(s) 199, 200, 221, 305
BsaHI GRCGYC 2 cut(s) 8, 336
BsaJI CCNNGG 3 cut(s) 198, 219, 390
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 391
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 1 cut(s) 427
BseDI CCNNGG 3 cut(s) 198, 219, 390
BseGI GGATG 3 cut(s) 569, 604, 610
BseLI CCNNNNNNNGG 2 cut(s) 163, 391
BseMII CTCAG 3 cut(s) 342, 531, 568
BseNI ACTGG 1 cut(s) 427
BsePI GCGCGC 1 cut(s) 145
BseRI GAGGAG 2 cut(s) 41, 202
BseXI GCAGC 3 cut(s) 52, 184, 393
BseYI CCCAGC 1 cut(s) 436
Bsh1236I CGCG 2 cut(s) 147, 149
BshFI GGCC 3 cut(s) 155, 262, 631
BshNI GGYRCC 1 cut(s) 7
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 4 cut(s) 37, 199, 221, 304
BsiWI CGTACG 1 cut(s) 21
BslI CCNNNNNNNGG 2 cut(s) 163, 391
BsmAI GTCTC 1 cut(s) 36
BsnI GGCC 3 cut(s) 155, 262, 631
BsoBI CYCGRG 1 cut(s) 198
Bsp1407I TGTACA 1 cut(s) 648
Bsp143I GATC 3 cut(s) 247, 374, 583
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 1 cut(s) 157
BspANI GGCC 3 cut(s) 155, 262, 631
BspCNI CTCAG 3 cut(s) 343, 532, 567
BspFNI CGCG 2 cut(s) 147, 149
BspHI TCATGA 1 cut(s) 586
BspLI GGNNCC 2 cut(s) 9, 376
BspMAI CTGCAG 1 cut(s) 461
BspPI GGATC 3 cut(s) 255, 369, 382
BspT107I GGYRCC 1 cut(s) 7
BspTI CTTAAG 1 cut(s) 104
BsrFI RCCGGY 1 cut(s) 36
BsrGI TGTACA 1 cut(s) 648
BsrI ACTGG 1 cut(s) 427
BssAI RCCGGY 1 cut(s) 36
BssECI CCNNGG 3 cut(s) 198, 219, 390
BssHII GCGCGC 1 cut(s) 145
BssMI GATC 3 cut(s) 247, 374, 583
BssNI GRCGYC 2 cut(s) 8, 336
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 390
Bst2BI CACGAG 1 cut(s) 176
Bst4CI ACNGT 1 cut(s) 163
BstACI GRCGYC 2 cut(s) 8, 336
BstAFI CTTAAG 1 cut(s) 104
BstAUI TGTACA 1 cut(s) 648
BstC8I GCNNGC 4 cut(s) 38, 93, 147, 157
BstDEI CTNAG 3 cut(s) 351, 540, 554
BstDSI CCRYGG 1 cut(s) 390
BstF5I GGATG 3 cut(s) 569, 604, 610
BstFNI CGCG 2 cut(s) 147, 149
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 3 cut(s) 10, 147, 149
BstKTI GATC 3 cut(s) 250, 377, 586
BstMAI GTCTC 1 cut(s) 36
BstMBI GATC 3 cut(s) 247, 374, 583
BstMWI GCNNNNNNNGC 1 cut(s) 637
BstSCI CCNGG 4 cut(s) 197, 198, 219, 303
BstSFI CTRYAG 1 cut(s) 457
BstUI CGCG 2 cut(s) 147, 149
BstV1I GCAGC 3 cut(s) 52, 184, 393
BstV2I GAAGAC 1 cut(s) 434
BstX2I RGATCY 1 cut(s) 374
BstXI CCANNNNNNTGG 1 cut(s) 422
BstYI RGATCY 1 cut(s) 374
BsuRI GGCC 3 cut(s) 155, 262, 631
BtgI CCRYGG 1 cut(s) 390
BtsCI GGATG 3 cut(s) 569, 604, 610
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 3 cut(s) 339, 420, 562
Cac8I GCNNGC 4 cut(s) 38, 93, 147, 157
CciI TCATGA 1 cut(s) 586
CfoI GCGC 3 cut(s) 10, 147, 149
Cfr10I RCCGGY 1 cut(s) 36
Cfr13I GGNCC 3 cut(s) 154, 192, 261
Cfr9I CCCGGG 1 cut(s) 198
CseI GACGC 1 cut(s) 344
Csp6I GTAC 2 cut(s) 22, 649
CviAII CATG 3 cut(s) 391, 416, 587
CviQI GTAC 2 cut(s) 22, 649
DdeI CTNAG 3 cut(s) 351, 540, 554
DinI GGCGCC 1 cut(s) 9
DpnI GATC 3 cut(s) 249, 376, 585
DpnII GATC 3 cut(s) 247, 374, 583
Eco130I CCWWGG 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 198
EcoO109I RGGNCCY 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 390
EcoT22I ATGCAT 1 cut(s) 610
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 3 cut(s) 394, 419, 590
FaiI YATR 9 cut(s) 89, 140, 288, 312, 392, 417, 530, 532, 588
FatI CATG 3 cut(s) 390, 415, 586
FauI CCCGC 1 cut(s) 164
FbaI TGATCA 1 cut(s) 583
Fnu4HI GCNGC 3 cut(s) 41, 173, 407
FokI GGATG 3 cut(s) 576, 611, 617
Fsp4HI GCNGC 3 cut(s) 41, 173, 407
GlaI GCGC 3 cut(s) 9, 146, 148
GluI GCNGC 3 cut(s) 41, 173, 407
GsaI CCCAGC 1 cut(s) 440
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 3 cut(s) 155, 262, 631
HapII CCGG 4 cut(s) 37, 199, 221, 304
HgaI GACGC 1 cut(s) 344
HhaI GCGC 3 cut(s) 10, 147, 149
Hin1I GRCGYC 2 cut(s) 8, 336
Hin1II CATG 3 cut(s) 394, 419, 590
Hin6I GCGC 3 cut(s) 8, 145, 147
HinP1I GCGC 3 cut(s) 8, 145, 147
HinfI GANTC 4 cut(s) 29, 362, 429, 552
HpaII CCGG 4 cut(s) 37, 199, 221, 304
HphI GGTGA 2 cut(s) 196, 406
Hpy166II GTNNAC 2 cut(s) 226, 649
Hpy188I TCNGA 5 cut(s) 216, 319, 367, 379, 447
Hpy188III TCNNGA 3 cut(s) 251, 472, 587
Hpy8I GTNNAC 2 cut(s) 226, 649
HpyAV CCTTC 2 cut(s) 145, 261
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4V TGCA 4 cut(s) 409, 459, 578, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 637
HpyF3I CTNAG 3 cut(s) 351, 540, 554
Hsp92I GRCGYC 2 cut(s) 8, 336
Hsp92II CATG 3 cut(s) 394, 419, 590
HspAI GCGC 3 cut(s) 8, 145, 147
KasI GGCGCC 1 cut(s) 7
KroI GCCGGC 1 cut(s) 36
KroNI GCCGGC 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 3 cut(s) 247, 374, 583
LpnPI CCDG 9 cut(s) 50, 212, 234, 277, 317, 340, 408, 418, 422
Lsp1109I GCAGC 3 cut(s) 52, 184, 393
LweI GCATC 2 cut(s) 393, 595
MaeIII GTNAC 1 cut(s) 418
MalI GATC 3 cut(s) 249, 376, 585
MboI GATC 3 cut(s) 247, 374, 583
MboII GAAGA 3 cut(s) 203, 434, 518
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 1 cut(s) 374
MluCI AATT 3 cut(s) 118, 579, 624
Mly113I GGCGCC 1 cut(s) 8
MlyI GAGTC 3 cut(s) 38, 371, 546
MmeI TCCRAC 1 cut(s) 239
Mph1103I ATGCAT 1 cut(s) 610
MroNI GCCGGC 1 cut(s) 36
MroXI GAANNNNTTC 2 cut(s) 271, 452
MseI TTAA 2 cut(s) 105, 656
MspCI CTTAAG 1 cut(s) 104
MspI CCGG 4 cut(s) 37, 199, 221, 304
MspR9I CCNGG 4 cut(s) 199, 200, 221, 305
MunI CAATTG 1 cut(s) 579
MvnI CGCG 2 cut(s) 147, 149
MwoI GCNNNNNNNGC 1 cut(s) 637
NaeI GCCGGC 1 cut(s) 38
NarI GGCGCC 1 cut(s) 8
NciI CCSGG 4 cut(s) 199, 200, 221, 305
NcoI CCATGG 1 cut(s) 390
NdeII GATC 3 cut(s) 247, 374, 583
NgoMIV GCCGGC 1 cut(s) 36
NlaIII CATG 3 cut(s) 394, 419, 590
NlaIV GGNNCC 2 cut(s) 9, 376
NmuCI GTSAC 1 cut(s) 418
NsiI ATGCAT 1 cut(s) 610
PagI TCATGA 1 cut(s) 586
PauI GCGCGC 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 40
PdiI GCCGGC 1 cut(s) 38
PdmI GAANNNNTTC 2 cut(s) 271, 452
PfeI GAWTC 1 cut(s) 429
Pfl23II CGTACG 1 cut(s) 21
PkrI GCNGC 3 cut(s) 42, 174, 408
PleI GAGTC 3 cut(s) 37, 370, 546
PluTI GGCGCC 1 cut(s) 11
PpsI GAGTC 3 cut(s) 37, 370, 546
PpuMI RGGWCCY 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 192
PspFI CCCAGC 1 cut(s) 436
PspLI CGTACG 1 cut(s) 21
PspN4I GGNNCC 2 cut(s) 9, 376
PspPI GGNCC 3 cut(s) 154, 192, 261
PspPPI RGGWCCY 1 cut(s) 192
PstI CTGCAG 1 cut(s) 461
PsuI RGATCY 1 cut(s) 374
PteI GCGCGC 1 cut(s) 145
RsaI GTAC 2 cut(s) 23, 650
RsaNI GTAC 2 cut(s) 22, 649
SaqAI TTAA 2 cut(s) 105, 656
SatI GCNGC 3 cut(s) 41, 173, 407
Sau3AI GATC 3 cut(s) 247, 374, 583
Sau96I GGNCC 3 cut(s) 154, 192, 261
SchI GAGTC 3 cut(s) 38, 371, 546
ScrFI CCNGG 4 cut(s) 199, 200, 221, 305
SfaNI GCATC 2 cut(s) 393, 595
SfcI CTRYAG 1 cut(s) 457
SfoI GGCGCC 1 cut(s) 9
SinI GGWCC 1 cut(s) 192
SmaI CCCGGG 1 cut(s) 200
SmlI CTYRAG 3 cut(s) 96, 104, 641
SmoI CTYRAG 3 cut(s) 96, 104, 641
Sse9I AATT 3 cut(s) 118, 579, 624
SsiI CCGC 1 cut(s) 157
SspDI GGCGCC 1 cut(s) 7
SspI AATATT 1 cut(s) 452
StyD4I CCNGG 4 cut(s) 197, 198, 219, 303
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 1 cut(s) 163
TasI AATT 3 cut(s) 118, 579, 624
TatI WGTACW 1 cut(s) 648
TfiI GAWTC 1 cut(s) 429
Tru1I TTAA 2 cut(s) 105, 656
Tru9I TTAA 2 cut(s) 105, 656
TscAI CASTG 3 cut(s) 346, 427, 562
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 3 cut(s) 40, 172, 406
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 3 cut(s) 155, 336, 519
TspMI CCCGGG 1 cut(s) 198
TspRI CASTG 3 cut(s) 346, 427, 562
Vha464I CTTAAG 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 1 cut(s) 118
XcmI CCANNNNNNNNNTGG 1 cut(s) 422
XmaI CCCGGG 1 cut(s) 198
XmnI GAANNNNTTC 2 cut(s) 271, 452
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.