FvH4_2g34591

B3 domain-containing protein At3g19184-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002837
Physical Location & Seq
Reverse (-)
25654863 .. 25658106
3244 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34591.t1

Sequence Viewer

Length: 651 bp
ATGGCGAAGCCAAACACGTCCTACGAAGAAACCCGAAAGAAGAGGATGGAAGAAAACAGGAAGAGAATGGAAGCCCTCAATCTGCCTCAGCTGGCTCAGGCGGCTTTTCCCAAACCCTCTCCGATGAAGCGCCAGAAGCCTCGCAAAGTGGAGAAGCAGATTGTTGTGGTCAGGAGGTCCGCTCGCGTCGCCACCCAGCCTACCCCTGTTTACAGAGAAGTTGAGGTGAAGCTACCAAGAAGCTATGGGAGGGTTTCAAAGCATAGGGATTTTTCCAACCGGGTATATGCTGATGAGCACTCCAGAGCTGAGGCCACTGAGAAAGCTGAGGCATTTGAGTCGGGTTTGGGGTCCGATTACCCAACCTTTGTTAAACCAATGCTCCAATCCCATGTCACTGGTGGATTCTGGATGGGTCTTCCACTCTCTTTCTGCAGGAAGTACCTCCCTAAGCGTGATGCAACTGTGACGTTGGTAGACGAACAAGGCGAGCAGTACCCTACTGTATACTTGGAGAACAAAAAAGGACTTAGCGGTGGATGGAGAGGATTTGCGATTGCTCATGAATTGGTGGATGGGGATGCATTGGTATTTCAGCTGACTAGGCCTACAACATTCAAGGTGTACATCACAAGGGTGGAAAGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

217

Amino Acids

24.74

Weight (kDa)

9.84

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 123 - 212 1.1e-18 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 182
AccI GTMKAC 2 cut(s) 477, 507
AccII CGCG 1 cut(s) 186
AciI CCGC 3 cut(s) 101, 180, 534
AfaI GTAC 3 cut(s) 443, 497, 626
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 2 cut(s) 258, 619
AjiI CACGTC 1 cut(s) 18
AleI CACNNNNGTG 1 cut(s) 635
AluBI AGCT 7 cut(s) 91, 232, 243, 308, 326, 598, 645
AluI AGCT 7 cut(s) 91, 232, 243, 308, 326, 598, 645
Alw21I GWGCWC 1 cut(s) 300
AoxI GGCC 2 cut(s) 312, 605
AspLEI GCGC 1 cut(s) 132
AspS9I GGNCC 2 cut(s) 177, 351
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 1 cut(s) 238
AvaII GGWCC 2 cut(s) 177, 351
BbsI GAAGAC 1 cut(s) 410
Bbv12I GWGCWC 1 cut(s) 300
BbvCI CCTCAGC 3 cut(s) 87, 309, 327
BccI CCATC 4 cut(s) 40, 406, 534, 569
BcgI CGANNNNNNTGC 2 cut(s) 321, 355
BcnI CCSGG 1 cut(s) 281
BfaI CTAG 1 cut(s) 603
BfmI CTRYAG 1 cut(s) 433
BfoI RGCGCY 1 cut(s) 133
BisI GCNGC 1 cut(s) 102
BlsI GCNGC 1 cut(s) 103
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 2 cut(s) 177, 351
BmgBI CACGTC 1 cut(s) 18
BmgT120I GGNCC 2 cut(s) 177, 351
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 2 cut(s) 448, 571
BpiI GAAGAC 1 cut(s) 410
BplI GAGNNNNNCTC 2 cut(s) 166, 198
BpmI CTGGAG 1 cut(s) 286
Bpu10I CCTNAGC 5 cut(s) 87, 96, 309, 327, 450
BpuMI CCSGG 1 cut(s) 281
BsaXI ACNNNNNCTCC 2 cut(s) 366, 396
Bse1I ACTGG 1 cut(s) 403
BseGI GGATG 5 cut(s) 51, 417, 545, 580, 586
BseMII CTCAG 5 cut(s) 101, 110, 300, 309, 318
BseNI ACTGG 1 cut(s) 403
BseYI CCCAGC 1 cut(s) 195
Bsh1236I CGCG 1 cut(s) 186
BshFI GGCC 2 cut(s) 314, 607
BsiHKAI GWGCWC 1 cut(s) 300
BsiSI CCGG 1 cut(s) 280
BsnI GGCC 2 cut(s) 314, 607
Bsp1286I GDGCHC 1 cut(s) 300
Bsp1407I TGTACA 1 cut(s) 624
BspACI CCGC 3 cut(s) 101, 180, 534
BspANI GGCC 2 cut(s) 314, 607
BspCNI CTCAG 5 cut(s) 100, 109, 301, 310, 319
BspFNI CGCG 1 cut(s) 186
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 1 cut(s) 352
BspMAI CTGCAG 1 cut(s) 437
BsrBI CCGCTC 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 624
BsrI ACTGG 1 cut(s) 403
BssNAI GTATAC 1 cut(s) 508
Bst1107I GTATAC 1 cut(s) 508
Bst4CI ACNGT 2 cut(s) 466, 505
Bst6I CTCTTC 2 cut(s) 35, 56
BstAUI TGTACA 1 cut(s) 624
BstC8I GCNNGC 3 cut(s) 93, 184, 491
BstDEI CTNAG 7 cut(s) 87, 96, 309, 318, 327, 450, 530
BstF5I GGATG 5 cut(s) 51, 417, 545, 580, 586
BstFNI CGCG 1 cut(s) 186
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 1 cut(s) 132
BstMWI GCNNNNNNNGC 4 cut(s) 101, 136, 188, 604
BstSCI CCNGG 1 cut(s) 279
BstSFI CTRYAG 1 cut(s) 433
BstUI CGCG 1 cut(s) 186
BstV2I GAAGAC 1 cut(s) 410
BstXI CCANNNNNNTGG 1 cut(s) 398
BstZ17I GTATAC 1 cut(s) 508
BsuRI GGCC 2 cut(s) 314, 607
BtrI CACGTC 1 cut(s) 18
BtsCI GGATG 5 cut(s) 51, 417, 545, 580, 586
BtsIMutI CAGTG 2 cut(s) 315, 396
Cac8I GCNNGC 3 cut(s) 93, 184, 491
CciI TCATGA 1 cut(s) 562
CfoI GCGC 1 cut(s) 132
Cfr13I GGNCC 2 cut(s) 177, 351
CseI GACGC 1 cut(s) 175
Csp6I GTAC 3 cut(s) 442, 496, 625
CviAII CATG 2 cut(s) 392, 563
CviQI GTAC 3 cut(s) 442, 496, 625
DdeI CTNAG 7 cut(s) 87, 96, 309, 318, 327, 450, 530
Eam1104I CTCTTC 2 cut(s) 35, 56
EarI CTCTTC 2 cut(s) 35, 56
Eco147I AGGCCT 1 cut(s) 607
Eco47I GGWCC 2 cut(s) 177, 351
EcoT22I ATGCAT 1 cut(s) 586
FaeI CATG 2 cut(s) 395, 566
FaiI YATR 7 cut(s) 246, 264, 286, 288, 393, 508, 564
FatI CATG 2 cut(s) 391, 562
FblI GTMKAC 2 cut(s) 477, 507
Fnu4HI GCNGC 1 cut(s) 102
FokI GGATG 5 cut(s) 58, 424, 552, 587, 593
Fsp4HI GCNGC 1 cut(s) 102
FspBI CTAG 1 cut(s) 603
GlaI GCGC 1 cut(s) 131
GluI GCNGC 1 cut(s) 102
GsaI CCCAGC 1 cut(s) 199
GsuI CTGGAG 1 cut(s) 286
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 2 cut(s) 314, 607
HapII CCGG 1 cut(s) 280
HgaI GACGC 1 cut(s) 175
HhaI GCGC 1 cut(s) 132
Hin1II CATG 2 cut(s) 395, 566
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HinfI GANTC 2 cut(s) 338, 405
HpaII CCGG 1 cut(s) 280
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 4 cut(s) 211, 478, 508, 625
Hpy188I TCNGA 2 cut(s) 123, 355
Hpy188III TCNNGA 4 cut(s) 172, 303, 409, 563
Hpy8I GTNNAC 4 cut(s) 211, 478, 508, 625
Hpy99I CGWCG 1 cut(s) 191
HpyCH4III ACNGT 2 cut(s) 466, 505
HpyCH4IV ACGT 2 cut(s) 17, 470
HpyCH4V TGCA 3 cut(s) 435, 461, 584
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 136, 188, 604
HpyF3I CTNAG 7 cut(s) 87, 96, 309, 318, 327, 450, 530
HpySE526I ACGT 2 cut(s) 17, 470
Hsp92II CATG 2 cut(s) 395, 566
HspAI GCGC 1 cut(s) 130
LmnI GCTCC 1 cut(s) 387
LweI GCATC 2 cut(s) 448, 571
MaeI CTAG 1 cut(s) 603
MaeII ACGT 2 cut(s) 17, 470
MaeIII GTNAC 2 cut(s) 394, 466
MbiI CCGCTC 1 cut(s) 182
MboII GAAGA 5 cut(s) 38, 52, 62, 73, 410
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 1 cut(s) 566
MlyI GAGTC 1 cut(s) 347
MmeI TCCRAC 1 cut(s) 300
Mph1103I ATGCAT 1 cut(s) 586
MseI TTAA 2 cut(s) 372, 649
MslI CAYNNNNRTG 1 cut(s) 635
MspA1I CMGCKG 2 cut(s) 91, 598
MspI CCGG 1 cut(s) 280
MspR9I CCNGG 1 cut(s) 281
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 4 cut(s) 101, 136, 188, 604
NciI CCSGG 1 cut(s) 281
NlaIII CATG 2 cut(s) 395, 566
NlaIV GGNNCC 1 cut(s) 352
NmuCI GTSAC 2 cut(s) 394, 466
NsiI ATGCAT 1 cut(s) 586
OliI CACNNNNGTG 1 cut(s) 635
PagI TCATGA 1 cut(s) 562
PceI AGGCCT 1 cut(s) 607
PcsI WCGNNNNNNNCGW 1 cut(s) 486
PfeI GAWTC 1 cut(s) 405
PkrI GCNGC 1 cut(s) 103
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PspFI CCCAGC 1 cut(s) 195
PspN4I GGNNCC 1 cut(s) 352
PspPI GGNCC 2 cut(s) 177, 351
PstI CTGCAG 1 cut(s) 437
PvuII CAGCTG 2 cut(s) 91, 598
RsaI GTAC 3 cut(s) 443, 497, 626
RsaNI GTAC 3 cut(s) 442, 496, 625
RseI CAYNNNNRTG 1 cut(s) 635
SaqAI TTAA 2 cut(s) 372, 649
SatI GCNGC 1 cut(s) 102
Sau96I GGNCC 2 cut(s) 177, 351
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 300
SfaNI GCATC 2 cut(s) 448, 571
SfcI CTRYAG 1 cut(s) 433
SinI GGWCC 2 cut(s) 177, 351
SmiMI CAYNNNNRTG 1 cut(s) 635
Sse9I AATT 1 cut(s) 566
SseBI AGGCCT 1 cut(s) 607
SsiI CCGC 3 cut(s) 101, 180, 534
SspMI CTAG 1 cut(s) 603
StuI AGGCCT 1 cut(s) 607
StyD4I CCNGG 1 cut(s) 279
TaaI ACNGT 2 cut(s) 466, 505
TaiI ACGT 2 cut(s) 20, 473
TasI AATT 1 cut(s) 566
TatI WGTACW 1 cut(s) 624
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 1 cut(s) 405
Tru1I TTAA 2 cut(s) 372, 649
Tru9I TTAA 2 cut(s) 372, 649
TscAI CASTG 2 cut(s) 322, 403
TseFI GTSAC 2 cut(s) 394, 466
Tsp45I GTSAC 2 cut(s) 394, 466
TspDTI ATGAA 2 cut(s) 140, 579
TspRI CASTG 2 cut(s) 322, 403
VpaK11BI GGWCC 2 cut(s) 177, 351
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XmiI GTMKAC 2 cut(s) 477, 507
XspI CTAG 1 cut(s) 603
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.