MD15G1084400.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002837
Physical Location & Seq
Reverse (-)
5833729 .. 5836526
2798 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1084400.v1.1.491

Sequence Viewer

Length: 681 bp
ATGGTGACGCCTAACTTATCATACGAGGAGTCTCGCCGGCAGCGAATGGAGGAAAACAAGAAGAGAATGGAAGCTCTGAATCTGCCTATGCTTGCTCAATCTCTTAAAAAAAACCCAAATTCTCCAAAACCCTCCCCTATGAAGCGCGCGAAGGCCCGCACGGTGGAGAAGCAGCTGGTGGAGGTGAGGAGGTCCTCCCGGGTGGCAAATCTTCCGACCCCAGTCTACAAAGAAGTGAAGGTTGTGGTGGATCGGGAGATGAGGCCCAGAAGGTATTCTACTAATAGACATAGGGATTTGTCGAACCGGGTTTATGCTTCGGATGAGGCCAGAGATGACGCCACTGCGAGAGCTGAGGCATTAGAGTCCGGTTTGGGATCCAGTTACCCGACCATGGTGAAACCGATGCTGCAATCCCATGTCACTGGTGGATTCTGGCTGGGTCTTCCAAAATATTTCTGCAGCAAGAACCTCCCGAAACGTGATGATATTGTGACTTTGATAGATGAAGATGAGGATGAGTGCAAGGTTATATACTTGGCTGAGAAAAGAGGACTCAGTGGTGGATGGAGAGGGTTCGCAATTGATCATGGTTTGGTGGATGGGGATGCATTGGTCTTTCAACTAATTCGGCCTAAAACACTCAAGGTGTACATTATAAGGGTGGAAAGAGCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

227

Amino Acids

25.9

Weight (kDa)

9.67

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 133 - 221 1.4e-15 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 659
AccI GTMKAC 1 cut(s) 225
AccII CGCG 2 cut(s) 147, 149
AciI CCGC 1 cut(s) 157
AclWI GGATC 3 cut(s) 258, 372, 385
AcsI RAATTY 1 cut(s) 118
AcyI GRCGYC 2 cut(s) 8, 339
AfaI GTAC 1 cut(s) 653
AfiI CCNNNNNNNGG 2 cut(s) 163, 394
AgsI TTSAA 1 cut(s) 623
AluBI AGCT 4 cut(s) 74, 175, 353, 674
AluI AGCT 4 cut(s) 74, 175, 353, 674
Alw26I GTCTC 1 cut(s) 36
AlwI GGATC 3 cut(s) 258, 372, 385
Ama87I CYCGRG 1 cut(s) 198
AoxI GGCC 4 cut(s) 153, 263, 327, 632
ApeKI GCWGC 4 cut(s) 40, 172, 409, 462
ApoI RAATTY 1 cut(s) 118
Asp700I GAANNNNTTC 1 cut(s) 274
AspLEI GCGC 2 cut(s) 147, 149
AspS9I GGNCC 3 cut(s) 154, 192, 264
AsuC2I CCSGG 3 cut(s) 199, 200, 308
AsuHPI GGTGA 3 cut(s) 16, 196, 409
AvaI CYCGRG 1 cut(s) 198
AvaII GGWCC 1 cut(s) 192
BamHI GGATCC 1 cut(s) 377
BarI GAAGNNNNNNTAC 2 cut(s) 262, 294
BbsI GAAGAC 1 cut(s) 437
BbvCI CCTCAGC 1 cut(s) 354
BbvI GCAGC 4 cut(s) 52, 184, 396, 474
BccI CCATC 2 cut(s) 561, 596
BclI TGATCA 1 cut(s) 586
BcnI CCSGG 3 cut(s) 199, 200, 308
BcoDI GTCTC 1 cut(s) 36
BfmI CTRYAG 1 cut(s) 460
BisI GCNGC 4 cut(s) 41, 173, 410, 463
BlsI GCNGC 4 cut(s) 42, 174, 411, 464
Bme1390I CCNGG 3 cut(s) 199, 200, 308
Bme18I GGWCC 1 cut(s) 192
BmeT110I CYCGRG 1 cut(s) 198
BmgT120I GGNCC 3 cut(s) 154, 192, 264
BmiI GGNNCC 1 cut(s) 379
BmrFI CCNGG 3 cut(s) 199, 200, 308
BmrI ACTGGG 1 cut(s) 215
BmsI GCATC 2 cut(s) 396, 598
BmuI ACTGGG 1 cut(s) 215
BoxI GACNNNNGTC 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 437
Bpu10I CCTNAGC 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 629
BpuMI CCSGG 3 cut(s) 199, 200, 308
BsaHI GRCGYC 2 cut(s) 8, 339
BsaJI CCNNGG 2 cut(s) 198, 393
BsaWI WCCGGW 1 cut(s) 368
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 394
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 3 cut(s) 221, 381, 430
BseDI CCNNGG 2 cut(s) 198, 393
BseGI GGATG 5 cut(s) 328, 523, 572, 607, 613
BseLI CCNNNNNNNGG 2 cut(s) 163, 394
BseMII CTCAG 3 cut(s) 345, 534, 571
BseNI ACTGG 3 cut(s) 221, 381, 430
BsePI GCGCGC 1 cut(s) 145
BseRI GAGGAG 2 cut(s) 41, 202
BseXI GCAGC 4 cut(s) 52, 184, 396, 474
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 2 cut(s) 147, 149
BshFI GGCC 4 cut(s) 155, 265, 329, 634
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 4 cut(s) 37, 199, 307, 369
BslI CCNNNNNNNGG 2 cut(s) 163, 394
BsmAI GTCTC 1 cut(s) 36
BsnI GGCC 4 cut(s) 155, 265, 329, 634
BsoBI CYCGRG 1 cut(s) 198
Bsp1407I TGTACA 1 cut(s) 651
Bsp143I GATC 3 cut(s) 250, 377, 586
Bsp19I CCATGG 1 cut(s) 393
BspACI CCGC 1 cut(s) 157
BspANI GGCC 4 cut(s) 155, 265, 329, 634
BspCNI CTCAG 3 cut(s) 346, 535, 570
BspFNI CGCG 2 cut(s) 147, 149
BspLI GGNNCC 1 cut(s) 379
BspMAI CTGCAG 1 cut(s) 464
BspPI GGATC 3 cut(s) 258, 372, 385
BsrFI RCCGGY 1 cut(s) 36
BsrGI TGTACA 1 cut(s) 651
BsrI ACTGG 3 cut(s) 221, 381, 430
BssAI RCCGGY 1 cut(s) 36
BssECI CCNNGG 2 cut(s) 198, 393
BssHII GCGCGC 1 cut(s) 145
BssMI GATC 3 cut(s) 250, 377, 586
BssNI GRCGYC 2 cut(s) 8, 339
BssT1I CCWWGG 1 cut(s) 393
Bst4CI ACNGT 1 cut(s) 163
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 2 cut(s) 8, 339
BstAUI TGTACA 1 cut(s) 651
BstC8I GCNNGC 4 cut(s) 38, 93, 147, 157
BstDEI CTNAG 3 cut(s) 354, 543, 557
BstDSI CCRYGG 1 cut(s) 393
BstF5I GGATG 5 cut(s) 328, 523, 572, 607, 613
BstFNI CGCG 2 cut(s) 147, 149
BstHHI GCGC 2 cut(s) 147, 149
BstKTI GATC 3 cut(s) 253, 380, 589
BstMAI GTCTC 1 cut(s) 36
BstMBI GATC 3 cut(s) 250, 377, 586
BstPAI GACNNNNGTC 1 cut(s) 221
BstSCI CCNGG 3 cut(s) 197, 198, 306
BstSFI CTRYAG 1 cut(s) 460
BstUI CGCG 2 cut(s) 147, 149
BstV1I GCAGC 4 cut(s) 52, 184, 396, 474
BstV2I GAAGAC 1 cut(s) 437
BstX2I RGATCY 1 cut(s) 377
BstXI CCANNNNNNTGG 1 cut(s) 425
BstYI RGATCY 1 cut(s) 377
BsuRI GGCC 4 cut(s) 155, 265, 329, 634
BtgI CCRYGG 1 cut(s) 393
BtsCI GGATG 5 cut(s) 328, 523, 572, 607, 613
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 3 cut(s) 342, 423, 565
Cac8I GCNNGC 4 cut(s) 38, 93, 147, 157
CfoI GCGC 2 cut(s) 147, 149
Cfr10I RCCGGY 1 cut(s) 36
Cfr13I GGNCC 3 cut(s) 154, 192, 264
Cfr9I CCCGGG 1 cut(s) 198
CseI GACGC 2 cut(s) 16, 347
Csp6I GTAC 1 cut(s) 652
CviAII CATG 3 cut(s) 394, 419, 590
CviQI GTAC 1 cut(s) 652
DdeI CTNAG 3 cut(s) 354, 543, 557
DpnI GATC 3 cut(s) 252, 379, 588
DpnII GATC 3 cut(s) 250, 377, 586
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 192
Eco88I CYCGRG 1 cut(s) 198
EcoO109I RGGNCCY 1 cut(s) 192
EcoT14I CCWWGG 1 cut(s) 393
EcoT22I ATGCAT 1 cut(s) 613
ErhI CCWWGG 1 cut(s) 393
FaeI CATG 3 cut(s) 397, 422, 593
FatI CATG 3 cut(s) 393, 418, 589
FauI CCCGC 1 cut(s) 164
FbaI TGATCA 1 cut(s) 586
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 4 cut(s) 41, 173, 410, 463
FokI GGATG 5 cut(s) 335, 530, 579, 614, 620
Fsp4HI GCNGC 4 cut(s) 41, 173, 410, 463
GlaI GCGC 2 cut(s) 146, 148
GluI GCNGC 4 cut(s) 41, 173, 410, 463
GsaI CCCAGC 1 cut(s) 443
HaeIII GGCC 4 cut(s) 155, 265, 329, 634
HapII CCGG 4 cut(s) 37, 199, 307, 369
HgaI GACGC 2 cut(s) 16, 347
HhaI GCGC 2 cut(s) 147, 149
Hin1I GRCGYC 2 cut(s) 8, 339
Hin1II CATG 3 cut(s) 397, 422, 593
Hin6I GCGC 2 cut(s) 145, 147
HinP1I GCGC 2 cut(s) 145, 147
HinfI GANTC 5 cut(s) 29, 79, 365, 432, 555
HpaII CCGG 4 cut(s) 37, 199, 307, 369
HphI GGTGA 3 cut(s) 16, 196, 409
Hpy166II GTNNAC 2 cut(s) 226, 652
Hpy188I TCNGA 3 cut(s) 78, 216, 322
Hpy188III TCNNGA 2 cut(s) 254, 475
Hpy8I GTNNAC 2 cut(s) 226, 652
HpyAV CCTTC 3 cut(s) 145, 232, 264
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4IV ACGT 1 cut(s) 481
HpyCH4V TGCA 4 cut(s) 412, 462, 525, 611
HpyF3I CTNAG 3 cut(s) 354, 543, 557
HpySE526I ACGT 1 cut(s) 481
Hsp92I GRCGYC 2 cut(s) 8, 339
Hsp92II CATG 3 cut(s) 397, 422, 593
HspAI GCGC 2 cut(s) 145, 147
KroI GCCGGC 1 cut(s) 36
KroNI GCCGGC 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 3 cut(s) 250, 377, 586
Lsp1109I GCAGC 4 cut(s) 52, 184, 396, 474
LweI GCATC 2 cut(s) 396, 598
MaeII ACGT 1 cut(s) 481
MaeIII GTNAC 4 cut(s) 4, 383, 421, 493
MalI GATC 3 cut(s) 252, 379, 588
MboI GATC 3 cut(s) 250, 377, 586
MboII GAAGA 4 cut(s) 73, 203, 437, 521
MfeI CAATTG 1 cut(s) 582
MflI RGATCY 1 cut(s) 377
MluCI AATT 3 cut(s) 118, 582, 627
MlyI GAGTC 3 cut(s) 38, 374, 549
MmeI TCCRAC 1 cut(s) 239
Mph1103I ATGCAT 1 cut(s) 613
MroNI GCCGGC 1 cut(s) 36
MroXI GAANNNNTTC 1 cut(s) 274
MseI TTAA 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 175
MspI CCGG 4 cut(s) 37, 199, 307, 369
MspR9I CCNGG 3 cut(s) 199, 200, 308
MunI CAATTG 1 cut(s) 582
MvnI CGCG 2 cut(s) 147, 149
NaeI GCCGGC 1 cut(s) 38
NciI CCSGG 3 cut(s) 199, 200, 308
NcoI CCATGG 1 cut(s) 393
NdeII GATC 3 cut(s) 250, 377, 586
NgoMIV GCCGGC 1 cut(s) 36
NlaIII CATG 3 cut(s) 397, 422, 593
NlaIV GGNNCC 1 cut(s) 379
NmuCI GTSAC 3 cut(s) 4, 421, 493
NsiI ATGCAT 1 cut(s) 613
PauI GCGCGC 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 40
PdiI GCCGGC 1 cut(s) 38
PdmI GAANNNNTTC 1 cut(s) 274
PfeI GAWTC 2 cut(s) 79, 432
PkrI GCNGC 4 cut(s) 42, 174, 411, 464
PleI GAGTC 3 cut(s) 37, 373, 549
PpsI GAGTC 3 cut(s) 37, 373, 549
PpuMI RGGWCCY 1 cut(s) 192
PshAI GACNNNNGTC 1 cut(s) 221
PsiI TTATAA 1 cut(s) 659
Psp5II RGGWCCY 1 cut(s) 192
PspFI CCCAGC 1 cut(s) 439
PspN4I GGNNCC 1 cut(s) 379
PspPI GGNCC 3 cut(s) 154, 192, 264
PspPPI RGGWCCY 1 cut(s) 192
PstI CTGCAG 1 cut(s) 464
PsuI RGATCY 1 cut(s) 377
PteI GCGCGC 1 cut(s) 145
PvuII CAGCTG 1 cut(s) 175
RsaI GTAC 1 cut(s) 653
RsaNI GTAC 1 cut(s) 652
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 4 cut(s) 41, 173, 410, 463
Sau3AI GATC 3 cut(s) 250, 377, 586
Sau96I GGNCC 3 cut(s) 154, 192, 264
SchI GAGTC 3 cut(s) 38, 374, 549
ScrFI CCNGG 3 cut(s) 199, 200, 308
SfaNI GCATC 2 cut(s) 396, 598
SfcI CTRYAG 1 cut(s) 460
SinI GGWCC 1 cut(s) 192
SmaI CCCGGG 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
Sse9I AATT 3 cut(s) 118, 582, 627
SsiI CCGC 1 cut(s) 157
SspI AATATT 1 cut(s) 455
StyD4I CCNGG 3 cut(s) 197, 198, 306
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 1 cut(s) 163
TaiI ACGT 1 cut(s) 484
TaqI TCGA 1 cut(s) 302
TasI AATT 3 cut(s) 118, 582, 627
TatI WGTACW 1 cut(s) 651
TfiI GAWTC 2 cut(s) 79, 432
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 3 cut(s) 349, 430, 565
TseFI GTSAC 3 cut(s) 4, 421, 493
TseI GCWGC 4 cut(s) 40, 172, 409, 462
Tsp45I GTSAC 3 cut(s) 4, 421, 493
TspDTI ATGAA 2 cut(s) 155, 522
TspMI CCCGGG 1 cut(s) 198
TspRI CASTG 3 cut(s) 349, 430, 565
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 1 cut(s) 118
XcmI CCANNNNNNNNNTGG 1 cut(s) 425
XmaI CCCGGG 1 cut(s) 198
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 274
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.