FvH4_2g38021

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013024
Physical Location & Seq
Reverse (-)
27563268 .. 27564001
734 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38021.t1

Sequence Viewer

Length: 429 bp
ATGCTCTTCTCCATCTTCCTAGCGCTCTTCCTACCCTGCGTAGGCATGAGCGCTGTGTTCGTGGTCTACATCTGTCTCTTATGGTTCGCCTCCAATTCCCGGTCGGCGGTAGCCCCTCAGTTACCGGTCAAGCACGTTTCCGAGAAGGGCTTATCGGCATCTGATCTCGAAAAGCTGCCGAAAACTAGCGGGAAAGAGCTGATCCGGGGGACGGAGTGCGCGGTGTGTCTGGACGAGATCGAGCCTGAGCAACCGGCCAGGGTGGTTCCCGGTTGTAACCACGGCTTCCATCTCCAATGCGCCGATACCTGGCTTTCTAAGAACTCGTGCTGTCCGGTTTGTCGAGCCAAACTCCAACCTCAGCAGATTTATGCTTCTTCTGATGACAATCCATACTGCGCAAGTACCAAAGCTGCCCCAATCAGTTAG

Protein Analysis

143

Amino Acids

15.41

Weight (kDa)

6.79

Isoelectric Point (pI)

57.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 72 - 115 5.4e-13 Ring finger domain
zf-RING_11 PF17123 72 - 100 2.3e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 72 - 114 1.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 73 - 114 8.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 86 - 115 1.1e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42200
fragaria_vesca FvH4_2g38021
malus_domestica MD08G1008600.v1.1 MD15G1007400.v1.1
prunus_persica Prupe.1G360300_v2.0.a1
pyrus_communis pycom08g00680
rosa_chinensis RchiOBHm_Chr6g0303581
rosa_laevigata RLG00000011067 RLG00000011071
rosa_multiflora Rmu_ssc0000114.1_g000026
rosa_roxburghii Rroxscaffold_7G00164610
rosa_rugosa Rorug06G0322500
rosa_samantha Rh6BG465900 Rh6BG466400 Rh6CG448900 Rh6DG436100
rosa_wichuraiana Rw6G037820 Rw6G037860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 400
AccI GTMKAC 1 cut(s) 66
AccII CGCG 1 cut(s) 221
AciI CCGC 3 cut(s) 107, 189, 221
AclWI GGATC 1 cut(s) 196
AcoI YGGCCR 1 cut(s) 255
AfaI GTAC 1 cut(s) 406
AfeI AGCGCT 2 cut(s) 24, 52
AfiI CCNNNNNNNGG 5 cut(s) 41, 99, 106, 211, 309
AgeI ACCGGT 1 cut(s) 124
AjnI CCWGG 2 cut(s) 257, 308
AluBI AGCT 3 cut(s) 175, 199, 413
AluI AGCT 3 cut(s) 175, 199, 413
Alw26I GTCTC 1 cut(s) 80
AlwI GGATC 1 cut(s) 196
Aor51HI AGCGCT 2 cut(s) 24, 52
AoxI GGCC 1 cut(s) 255
ApeKI GCWGC 2 cut(s) 175, 413
AsiGI ACCGGT 1 cut(s) 124
AspLEI GCGC 5 cut(s) 25, 53, 221, 302, 401
AsuC2I CCSGG 3 cut(s) 100, 206, 270
BauI CACGAG 1 cut(s) 325
BbvCI CCTCAGC 1 cut(s) 360
BbvI GCAGC 2 cut(s) 162, 400
BccI CCATC 2 cut(s) 20, 297
BceAI ACGGC 1 cut(s) 298
BciT130I CCWGG 2 cut(s) 259, 310
BcnI CCSGG 3 cut(s) 100, 206, 270
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 2 cut(s) 20, 186
BfoI RGCGCY 2 cut(s) 26, 54
BisI GCNGC 2 cut(s) 176, 414
BlsI GCNGC 2 cut(s) 177, 415
Bme1390I CCNGG 5 cut(s) 100, 206, 259, 270, 310
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 5 cut(s) 100, 206, 259, 270, 310
BmsI GCATC 1 cut(s) 167
BplI GAGNNNNNCTC 2 cut(s) 336, 368
Bpu10I CCTNAGC 2 cut(s) 246, 360
BpuMI CCSGG 3 cut(s) 100, 206, 270
BsaBI GATNNNNATC 1 cut(s) 387
BsaJI CCNNGG 3 cut(s) 205, 258, 280
BsaWI WCCGGW 2 cut(s) 124, 334
Bsc4I CCNNNNNNNGG 5 cut(s) 41, 99, 106, 211, 309
Bse118I RCCGGY 2 cut(s) 124, 253
Bse8I GATNNNNATC 1 cut(s) 387
BseBI CCWGG 2 cut(s) 259, 310
BseDI CCNNGG 3 cut(s) 205, 258, 280
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 5 cut(s) 41, 99, 106, 211, 309
BseMII CTCAG 3 cut(s) 131, 237, 374
BseXI GCAGC 2 cut(s) 162, 400
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 104
BshFI GGCC 1 cut(s) 257
BshTI ACCGGT 1 cut(s) 124
BsiEI CGRYCG 1 cut(s) 104
BsiSI CCGG 6 cut(s) 100, 125, 205, 254, 270, 335
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 5 cut(s) 41, 99, 106, 211, 309
BsmAI GTCTC 1 cut(s) 80
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 1 cut(s) 257
Bsp143I GATC 3 cut(s) 163, 201, 237
BspACI CCGC 3 cut(s) 107, 189, 221
BspANI GGCC 1 cut(s) 257
BspCNI CTCAG 3 cut(s) 130, 238, 373
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 1 cut(s) 267
BspPI GGATC 1 cut(s) 196
BspQI GCTCTTC 2 cut(s) 11, 32
BsrFI RCCGGY 2 cut(s) 124, 253
BssAI RCCGGY 2 cut(s) 124, 253
BssECI CCNNGG 3 cut(s) 205, 258, 280
BssMI GATC 3 cut(s) 163, 201, 237
BssSI CACGAG 1 cut(s) 325
Bst2BI CACGAG 1 cut(s) 325
Bst2UI CCWGG 2 cut(s) 259, 310
Bst6I CTCTTC 2 cut(s) 11, 32
BstDEI CTNAG 4 cut(s) 117, 246, 318, 360
BstDSI CCRYGG 1 cut(s) 280
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 2 cut(s) 26, 54
BstHHI GCGC 5 cut(s) 25, 53, 221, 302, 401
BstKTI GATC 3 cut(s) 166, 204, 240
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 3 cut(s) 163, 201, 237
BstMCI CGRYCG 1 cut(s) 104
BstNI CCWGG 2 cut(s) 259, 310
BstSCI CCNGG 5 cut(s) 98, 204, 257, 268, 308
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 2 cut(s) 162, 400
BsuRI GGCC 1 cut(s) 257
BtgI CCRYGG 1 cut(s) 280
CfoI GCGC 5 cut(s) 25, 53, 221, 302, 401
Cfr10I RCCGGY 2 cut(s) 124, 253
Csp6I GTAC 1 cut(s) 405
CspAI ACCGGT 1 cut(s) 124
CviAII CATG 1 cut(s) 46
CviQI GTAC 1 cut(s) 405
DdeI CTNAG 4 cut(s) 117, 246, 318, 360
DpnI GATC 3 cut(s) 165, 203, 239
DpnII GATC 3 cut(s) 163, 201, 237
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 2 cut(s) 11, 32
EarI CTCTTC 2 cut(s) 11, 32
Eco47III AGCGCT 2 cut(s) 24, 52
EcoRII CCWGG 2 cut(s) 257, 308
FaeI CATG 1 cut(s) 49
FaiI YATR 4 cut(s) 47, 82, 372, 394
FaqI GGGAC 1 cut(s) 223
FatI CATG 1 cut(s) 45
FauI CCCGC 1 cut(s) 182
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 176, 414
Fsp4HI GCNGC 2 cut(s) 176, 414
FspBI CTAG 2 cut(s) 20, 186
FspI TGCGCA 1 cut(s) 400
GlaI GCGC 5 cut(s) 24, 52, 220, 301, 400
GluI GCNGC 2 cut(s) 176, 414
HaeII RGCGCY 2 cut(s) 26, 54
HaeIII GGCC 1 cut(s) 257
HapII CCGG 6 cut(s) 100, 125, 205, 254, 270, 335
HhaI GCGC 5 cut(s) 25, 53, 221, 302, 401
Hin1II CATG 1 cut(s) 49
Hin6I GCGC 5 cut(s) 23, 51, 219, 300, 399
HinP1I GCGC 5 cut(s) 23, 51, 219, 300, 399
HpaII CCGG 6 cut(s) 100, 125, 205, 254, 270, 335
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 3 cut(s) 142, 163, 382
Hpy188III TCNNGA 2 cut(s) 167, 230
Hpy8I GTNNAC 1 cut(s) 67
HpyAV CCTTC 1 cut(s) 139
HpyCH4IV ACGT 1 cut(s) 135
HpyF3I CTNAG 4 cut(s) 117, 246, 318, 360
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 1 cut(s) 49
HspAI GCGC 5 cut(s) 23, 51, 219, 300, 399
Kzo9I GATC 3 cut(s) 163, 201, 237
LguI GCTCTTC 2 cut(s) 11, 32
Lsp1109I GCAGC 2 cut(s) 162, 400
LweI GCATC 1 cut(s) 167
MaeI CTAG 2 cut(s) 20, 186
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 2 cut(s) 120, 275
MalI GATC 3 cut(s) 165, 203, 239
MboI GATC 3 cut(s) 163, 201, 237
MboII GAAGA 3 cut(s) 7, 19, 369
MluCI AATT 1 cut(s) 94
MmeI TCCRAC 1 cut(s) 379
MnlI CCTC 3 cut(s) 100, 126, 369
MspI CCGG 6 cut(s) 100, 125, 205, 254, 270, 335
MspR9I CCNGG 5 cut(s) 100, 206, 259, 270, 310
MvaI CCWGG 2 cut(s) 259, 310
MvnI CGCG 1 cut(s) 221
NciI CCSGG 3 cut(s) 100, 206, 270
NdeII GATC 3 cut(s) 163, 201, 237
NlaIII CATG 1 cut(s) 49
NlaIV GGNNCC 1 cut(s) 267
NsbI TGCGCA 1 cut(s) 400
PciSI GCTCTTC 2 cut(s) 11, 32
PinAI ACCGGT 1 cut(s) 124
PkrI GCNGC 2 cut(s) 177, 415
Psp6I CCWGG 2 cut(s) 257, 308
PspGI CCWGG 2 cut(s) 257, 308
PspN4I GGNNCC 1 cut(s) 267
RsaI GTAC 1 cut(s) 406
RsaNI GTAC 1 cut(s) 405
SapI GCTCTTC 2 cut(s) 11, 32
SatI GCNGC 2 cut(s) 176, 414
Sau3AI GATC 3 cut(s) 163, 201, 237
ScrFI CCNGG 5 cut(s) 100, 206, 259, 270, 310
SetI ASST 6 cut(s) 138, 177, 201, 311, 361, 415
SfaNI GCATC 1 cut(s) 167
Sse9I AATT 1 cut(s) 94
SsiI CCGC 3 cut(s) 107, 189, 221
SspMI CTAG 2 cut(s) 20, 186
StyD4I CCNGG 5 cut(s) 98, 204, 257, 268, 308
TaiI ACGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 168, 240, 343
TasI AATT 1 cut(s) 94
TseI GCWGC 2 cut(s) 175, 413
TspGWI ACGGA 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 66
XspI CTAG 2 cut(s) 20, 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.