Rw6G037820

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0013024
Physical Location & Seq
Forward (+)
62091274 .. 62091672
399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G037820.1

Sequence Viewer

Length: 363 bp
ATGCTCTTCTCCATCTTCTTATTGCTTTTCCTACCCTGCGTAGGCATGAGCGCTGTGTTCGTGGTCTACATCTGTCTCTTATGGTTCGCCGCCAATACCCGGTCGTCGAAGGGCTTGTCGGCCTCTGATCTCGAAAAGTTGCCGAAAACTAGCGGCAAAGAGCTGACCAGGGGGACGGAGTGCGCGGTGTGCCTGGAGGAGATCGAGGCAGAGCAACCGGCTCGGGTGGTTCCAGGTTGTAACCACGGCTTCCATCTCCAATGCGCCGATACCTGGCTTTCTAAGCAGTCATTCTGTCCGGTTTGCAGAGCCAAACTCCAACCTCAGCAGTTTCTCACTTCCTCAGATGAAAATCCATGCTAG

Protein Analysis

120

Amino Acids

13.25

Weight (kDa)

6.07

Isoelectric Point (pI)

54.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 60 - 103 6.1e-14 Ring finger domain
zf-RING_11 PF17123 60 - 88 1e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 60 - 102 4.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 61 - 103 1.5e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 61 - 102 2.6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42200
fragaria_vesca FvH4_2g38021
malus_domestica MD08G1008600.v1.1 MD15G1007400.v1.1
prunus_persica Prupe.1G360300_v2.0.a1
pyrus_communis pycom08g00680
rosa_chinensis RchiOBHm_Chr6g0303581
rosa_laevigata RLG00000011067 RLG00000011071
rosa_multiflora Rmu_ssc0000114.1_g000026
rosa_roxburghii Rroxscaffold_7G00164610
rosa_rugosa Rorug06G0322500
rosa_samantha Rh6BG465900 Rh6BG466400 Rh6CG448900 Rh6DG436100
rosa_wichuraiana Rw6G037820 Rw6G037860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 66
AccII CGCG 1 cut(s) 185
AciI CCGC 3 cut(s) 90, 153, 185
AfeI AGCGCT 1 cut(s) 52
AfiI CCNNNNNNNGG 3 cut(s) 41, 99, 273
AjnI CCWGG 4 cut(s) 167, 192, 232, 272
AluBI AGCT 1 cut(s) 163
AluI AGCT 1 cut(s) 163
Alw26I GTCTC 1 cut(s) 80
Ama87I CYCGRG 1 cut(s) 222
Aor51HI AGCGCT 1 cut(s) 52
AoxI GGCC 1 cut(s) 120
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
AspLEI GCGC 3 cut(s) 53, 185, 266
AsuC2I CCSGG 1 cut(s) 100
AvaI CYCGRG 1 cut(s) 222
BbvCI CCTCAGC 1 cut(s) 324
BccI CCATC 2 cut(s) 20, 261
BceAI ACGGC 1 cut(s) 262
BcgI CGANNNNNNTGC 2 cut(s) 203, 237
BciT130I CCWGG 4 cut(s) 169, 194, 234, 274
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 2 cut(s) 150, 361
BfoI RGCGCY 1 cut(s) 54
BisI GCNGC 2 cut(s) 90, 154
BlsI GCNGC 2 cut(s) 91, 155
Bme1390I CCNGG 5 cut(s) 100, 169, 194, 234, 274
BmeT110I CYCGRG 1 cut(s) 222
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 5 cut(s) 100, 169, 194, 234, 274
BplI GAGNNNNNCTC 2 cut(s) 300, 332
BpmI CTGGAG 1 cut(s) 215
Bpu10I CCTNAGC 1 cut(s) 324
BpuMI CCSGG 1 cut(s) 100
BsaBI GATNNNNATC 1 cut(s) 351
BsaJI CCNNGG 2 cut(s) 168, 244
BsaWI WCCGGW 1 cut(s) 298
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 99, 273
Bse118I RCCGGY 1 cut(s) 217
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 4 cut(s) 169, 194, 234, 274
BseDI CCNNGG 2 cut(s) 168, 244
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 3 cut(s) 41, 99, 273
BseMII CTCAG 2 cut(s) 338, 357
BseRI GAGGAG 1 cut(s) 212
Bsh1236I CGCG 1 cut(s) 185
Bsh1285I CGRYCG 1 cut(s) 104
BshFI GGCC 1 cut(s) 122
BsiEI CGRYCG 1 cut(s) 104
BsiHKCI CYCGRG 1 cut(s) 222
BsiSI CCGG 3 cut(s) 100, 218, 299
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 3 cut(s) 41, 99, 273
BsmAI GTCTC 1 cut(s) 80
BsmFI GGGAC 1 cut(s) 187
BsnI GGCC 1 cut(s) 122
BsoBI CYCGRG 1 cut(s) 222
Bsp143I GATC 2 cut(s) 127, 201
BspACI CCGC 3 cut(s) 90, 153, 185
BspANI GGCC 1 cut(s) 122
BspCNI CTCAG 2 cut(s) 337, 356
BspFNI CGCG 1 cut(s) 185
BspLI GGNNCC 1 cut(s) 231
BspQI GCTCTTC 1 cut(s) 11
BsrFI RCCGGY 1 cut(s) 217
BssAI RCCGGY 1 cut(s) 217
BssECI CCNNGG 2 cut(s) 168, 244
BssMI GATC 2 cut(s) 127, 201
Bst2UI CCWGG 4 cut(s) 169, 194, 234, 274
Bst6I CTCTTC 1 cut(s) 11
BstDEI CTNAG 3 cut(s) 282, 324, 343
BstDSI CCRYGG 1 cut(s) 244
BstFNI CGCG 1 cut(s) 185
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 3 cut(s) 53, 185, 266
BstKTI GATC 2 cut(s) 130, 204
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 2 cut(s) 127, 201
BstMCI CGRYCG 1 cut(s) 104
BstMWI GCNNNNNNNGC 2 cut(s) 189, 283
BstNI CCWGG 4 cut(s) 169, 194, 234, 274
BstSCI CCNGG 5 cut(s) 98, 167, 192, 232, 272
BstUI CGCG 1 cut(s) 185
BsuRI GGCC 1 cut(s) 122
BtgI CCRYGG 1 cut(s) 244
CfoI GCGC 3 cut(s) 53, 185, 266
Cfr10I RCCGGY 1 cut(s) 217
CviAII CATG 2 cut(s) 46, 357
CviJI RGCY 7 cut(s) 114, 122, 163, 221, 249, 277, 311
CviKI_1 RGCY 7 cut(s) 114, 122, 163, 221, 249, 277, 311
DdeI CTNAG 3 cut(s) 282, 324, 343
DpnI GATC 2 cut(s) 129, 203
DpnII GATC 2 cut(s) 127, 201
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
Eco47III AGCGCT 1 cut(s) 52
Eco88I CYCGRG 1 cut(s) 222
EcoRII CCWGG 4 cut(s) 167, 192, 232, 272
FaeI CATG 2 cut(s) 49, 360
FaiI YATR 3 cut(s) 47, 82, 358
FaqI GGGAC 1 cut(s) 187
FatI CATG 2 cut(s) 45, 356
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 90, 154
Fsp4HI GCNGC 2 cut(s) 90, 154
FspBI CTAG 2 cut(s) 150, 361
GlaI GCGC 3 cut(s) 52, 184, 265
GluI GCNGC 2 cut(s) 90, 154
GsuI CTGGAG 1 cut(s) 215
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 122
HapII CCGG 3 cut(s) 100, 218, 299
HhaI GCGC 3 cut(s) 53, 185, 266
Hin1II CATG 2 cut(s) 49, 360
Hin6I GCGC 3 cut(s) 51, 183, 264
HinP1I GCGC 3 cut(s) 51, 183, 264
HpaII CCGG 3 cut(s) 100, 218, 299
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 2 cut(s) 127, 346
Hpy188III TCNNGA 1 cut(s) 131
Hpy8I GTNNAC 1 cut(s) 67
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 1 cut(s) 103
HpyCH4V TGCA 1 cut(s) 306
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 283
HpyF3I CTNAG 3 cut(s) 282, 324, 343
Hsp92II CATG 2 cut(s) 49, 360
HspAI GCGC 3 cut(s) 51, 183, 264
Kzo9I GATC 2 cut(s) 127, 201
LguI GCTCTTC 1 cut(s) 11
MaeI CTAG 2 cut(s) 150, 361
MaeIII GTNAC 1 cut(s) 239
MalI GATC 2 cut(s) 129, 203
MboI GATC 2 cut(s) 127, 201
MboII GAAGA 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 5 cut(s) 133, 190, 199, 333, 352
MspI CCGG 3 cut(s) 100, 218, 299
MspR9I CCNGG 5 cut(s) 100, 169, 194, 234, 274
MvaI CCWGG 4 cut(s) 169, 194, 234, 274
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 2 cut(s) 189, 283
NciI CCSGG 1 cut(s) 100
NdeII GATC 2 cut(s) 127, 201
NlaIII CATG 2 cut(s) 49, 360
NlaIV GGNNCC 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 11
PkrI GCNGC 2 cut(s) 91, 155
Psp6I CCWGG 4 cut(s) 167, 192, 232, 272
PspGI CCWGG 4 cut(s) 167, 192, 232, 272
PspN4I GGNNCC 1 cut(s) 231
SapI GCTCTTC 1 cut(s) 11
SatI GCNGC 2 cut(s) 90, 154
Sau3AI GATC 2 cut(s) 127, 201
ScrFI CCNGG 5 cut(s) 100, 169, 194, 234, 274
SetI ASST 4 cut(s) 165, 238, 275, 325
SsiI CCGC 3 cut(s) 90, 153, 185
SspMI CTAG 2 cut(s) 150, 361
StyD4I CCNGG 5 cut(s) 98, 167, 192, 232, 272
TaqI TCGA 3 cut(s) 107, 132, 204
TauI GCSGC 2 cut(s) 92, 156
TspDTI ATGAA 1 cut(s) 363
TspGWI ACGGA 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 66
XspI CTAG 2 cut(s) 150, 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.