Rh6CG448900

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0013024
Physical Location & Seq
Forward (+)
62685774 .. 62686127
354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG448900.1

Sequence Viewer

Length: 354 bp
ATGAGCGCTGTGTTCGTGGTCTACATCTGTCTCTTATGGTTCGCCGCCAATACCCGGTCGTCGGCAGAGCCTCGGTTACCGGTCAAGCTGGTTTCCGAGAAGGGCTTGTCGGCCTCTGATCTCGACAAGTTGCCGAAAACTAGCGGCAAAGAGCTGACCAGGGGGACGGAGTGCGCGGTGTGCCTGGAGGAGATCGAGGCAGAGCAACCGGCTCGGGTGGTTCCAGGTTGTAACCACGGCTTCCATCTCCAATGCGCCGATACCTGGCTTTCTAAGCAGTCATTCTGTCCGGTTTGCAGAGCCAAACTCCAACCTCAGCAGTTTCTCACTTCCTCTTATGAAAATCCATGCTAG

Protein Analysis

117

Amino Acids

12.91

Weight (kDa)

6.7

Isoelectric Point (pI)

60.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 57 - 100 5.8e-14 Ring finger domain
zf-RING_11 PF17123 57 - 85 9.6e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 57 - 99 4.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 58 - 100 1.5e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 58 - 99 2.5e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42200
fragaria_vesca FvH4_2g38021
malus_domestica MD08G1008600.v1.1 MD15G1007400.v1.1
prunus_persica Prupe.1G360300_v2.0.a1
pyrus_communis pycom08g00680
rosa_chinensis RchiOBHm_Chr6g0303581
rosa_laevigata RLG00000011067 RLG00000011071
rosa_multiflora Rmu_ssc0000114.1_g000026
rosa_roxburghii Rroxscaffold_7G00164610
rosa_rugosa Rorug06G0322500
rosa_samantha Rh6BG465900 Rh6BG466400 Rh6CG448900 Rh6DG436100
rosa_wichuraiana Rw6G037820 Rw6G037860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 21
AccII CGCG 1 cut(s) 176
AciI CCGC 3 cut(s) 45, 144, 176
AfeI AGCGCT 1 cut(s) 7
AfiI CCNNNNNNNGG 3 cut(s) 54, 61, 264
AgeI ACCGGT 1 cut(s) 79
AjnI CCWGG 4 cut(s) 158, 183, 223, 263
AluBI AGCT 2 cut(s) 88, 154
AluI AGCT 2 cut(s) 88, 154
Alw26I GTCTC 1 cut(s) 35
Ama87I CYCGRG 1 cut(s) 213
Aor51HI AGCGCT 1 cut(s) 7
AoxI GGCC 1 cut(s) 111
AsiGI ACCGGT 1 cut(s) 79
AspLEI GCGC 3 cut(s) 8, 176, 257
AsuC2I CCSGG 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 213
BbvCI CCTCAGC 1 cut(s) 315
BccI CCATC 1 cut(s) 252
BceAI ACGGC 1 cut(s) 253
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciT130I CCWGG 4 cut(s) 160, 185, 225, 265
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 35
BfaI CTAG 2 cut(s) 141, 352
BfoI RGCGCY 1 cut(s) 9
BisI GCNGC 2 cut(s) 45, 145
BlsI GCNGC 2 cut(s) 46, 146
Bme1390I CCNGG 5 cut(s) 55, 160, 185, 225, 265
BmeT110I CYCGRG 1 cut(s) 213
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 5 cut(s) 55, 160, 185, 225, 265
BplI GAGNNNNNCTC 2 cut(s) 291, 323
BpmI CTGGAG 1 cut(s) 206
Bpu10I CCTNAGC 1 cut(s) 315
BpuMI CCSGG 1 cut(s) 55
BsaJI CCNNGG 3 cut(s) 71, 159, 235
BsaWI WCCGGW 2 cut(s) 79, 289
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 61, 264
Bse118I RCCGGY 2 cut(s) 79, 208
BseBI CCWGG 4 cut(s) 160, 185, 225, 265
BseDI CCNNGG 3 cut(s) 71, 159, 235
BseLI CCNNNNNNNGG 3 cut(s) 54, 61, 264
BseMII CTCAG 1 cut(s) 329
BseRI GAGGAG 1 cut(s) 203
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 59
BshFI GGCC 1 cut(s) 113
BshTI ACCGGT 1 cut(s) 79
BsiEI CGRYCG 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 213
BsiSI CCGG 4 cut(s) 55, 80, 209, 290
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 3 cut(s) 54, 61, 264
BsmAI GTCTC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 178
BsnI GGCC 1 cut(s) 113
BsoBI CYCGRG 1 cut(s) 213
Bsp143I GATC 2 cut(s) 118, 192
BspACI CCGC 3 cut(s) 45, 144, 176
BspANI GGCC 1 cut(s) 113
BspCNI CTCAG 1 cut(s) 328
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 1 cut(s) 222
BsrFI RCCGGY 2 cut(s) 79, 208
BssAI RCCGGY 2 cut(s) 79, 208
BssECI CCNNGG 3 cut(s) 71, 159, 235
BssMI GATC 2 cut(s) 118, 192
Bst2UI CCWGG 4 cut(s) 160, 185, 225, 265
BstDEI CTNAG 2 cut(s) 273, 315
BstDSI CCRYGG 1 cut(s) 235
BstEII GGTNACC 1 cut(s) 75
BstFNI CGCG 1 cut(s) 176
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 3 cut(s) 8, 176, 257
BstKTI GATC 2 cut(s) 121, 195
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 2 cut(s) 118, 192
BstMCI CGRYCG 1 cut(s) 59
BstMWI GCNNNNNNNGC 2 cut(s) 180, 274
BstNI CCWGG 4 cut(s) 160, 185, 225, 265
BstPI GGTNACC 1 cut(s) 75
BstSCI CCNGG 5 cut(s) 53, 158, 183, 223, 263
BstUI CGCG 1 cut(s) 176
BsuRI GGCC 1 cut(s) 113
BtgI CCRYGG 1 cut(s) 235
CfoI GCGC 3 cut(s) 8, 176, 257
Cfr10I RCCGGY 2 cut(s) 79, 208
CspAI ACCGGT 1 cut(s) 79
CviAII CATG 1 cut(s) 348
CviJI RGCY 9 cut(s) 70, 88, 105, 113, 154, 212, 240, 268, 302
CviKI_1 RGCY 9 cut(s) 70, 88, 105, 113, 154, 212, 240, 268, 302
DdeI CTNAG 2 cut(s) 273, 315
DpnI GATC 2 cut(s) 120, 194
DpnII GATC 2 cut(s) 118, 192
Eco47III AGCGCT 1 cut(s) 7
Eco88I CYCGRG 1 cut(s) 213
Eco91I GGTNACC 1 cut(s) 75
EcoO65I GGTNACC 1 cut(s) 75
EcoRII CCWGG 4 cut(s) 158, 183, 223, 263
FaeI CATG 1 cut(s) 351
FaiI YATR 3 cut(s) 37, 339, 349
FaqI GGGAC 1 cut(s) 178
FatI CATG 1 cut(s) 347
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 2 cut(s) 45, 145
Fsp4HI GCNGC 2 cut(s) 45, 145
FspBI CTAG 2 cut(s) 141, 352
GlaI GCGC 3 cut(s) 7, 175, 256
GluI GCNGC 2 cut(s) 45, 145
GsuI CTGGAG 1 cut(s) 206
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 1 cut(s) 113
HapII CCGG 4 cut(s) 55, 80, 209, 290
HhaI GCGC 3 cut(s) 8, 176, 257
Hin1II CATG 1 cut(s) 351
Hin6I GCGC 3 cut(s) 6, 174, 255
HinP1I GCGC 3 cut(s) 6, 174, 255
HpaII CCGG 4 cut(s) 55, 80, 209, 290
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 2 cut(s) 97, 118
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 1 cut(s) 22
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 1 cut(s) 94
HpyCH4V TGCA 1 cut(s) 297
HpyF10VI GCNNNNNNNGC 2 cut(s) 180, 274
HpyF3I CTNAG 2 cut(s) 273, 315
Hsp92II CATG 1 cut(s) 351
HspAI GCGC 3 cut(s) 6, 174, 255
Kzo9I GATC 2 cut(s) 118, 192
MaeI CTAG 2 cut(s) 141, 352
MaeIII GTNAC 2 cut(s) 75, 230
MalI GATC 2 cut(s) 120, 194
MboI GATC 2 cut(s) 118, 192
MmeI TCCRAC 1 cut(s) 334
MnlI CCTC 6 cut(s) 81, 124, 181, 190, 324, 343
MspI CCGG 4 cut(s) 55, 80, 209, 290
MspR9I CCNGG 5 cut(s) 55, 160, 185, 225, 265
MvaI CCWGG 4 cut(s) 160, 185, 225, 265
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 180, 274
NciI CCSGG 1 cut(s) 55
NdeII GATC 2 cut(s) 118, 192
NlaIII CATG 1 cut(s) 351
NlaIV GGNNCC 1 cut(s) 222
PinAI ACCGGT 1 cut(s) 79
PkrI GCNGC 2 cut(s) 46, 146
Psp6I CCWGG 4 cut(s) 158, 183, 223, 263
PspEI GGTNACC 1 cut(s) 75
PspGI CCWGG 4 cut(s) 158, 183, 223, 263
PspN4I GGNNCC 1 cut(s) 222
SatI GCNGC 2 cut(s) 45, 145
Sau3AI GATC 2 cut(s) 118, 192
ScrFI CCNGG 5 cut(s) 55, 160, 185, 225, 265
SetI ASST 5 cut(s) 90, 156, 229, 266, 316
SsiI CCGC 3 cut(s) 45, 144, 176
SspMI CTAG 2 cut(s) 141, 352
StyD4I CCNGG 5 cut(s) 53, 158, 183, 223, 263
TaqI TCGA 2 cut(s) 123, 195
TauI GCSGC 2 cut(s) 47, 147
TspDTI ATGAA 1 cut(s) 354
TspGWI ACGGA 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 21
XspI CTAG 2 cut(s) 141, 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.