FvH4_2g38330

Protein of unknown function (DUF615)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011405
Physical Location & Seq
Reverse (-)
27751317 .. 27753464
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38330.t1

Sequence Viewer

Length: 903 bp
ATGGCTCAACTGGGGAGACTGCTGGTAAGGCAATGCCCGGCGCTGCAGCACCTGTGCTGCTCCTGCGTCGCACTGAATCATCTCTTCTTCTCAACCTCGCTTCCACTGAGCACAAACCCTAATCCCCGCCGCGGTTCTTCTCCGGTAATCAACTTAGCTACTCGCGGCGTCCATAAACTAGTCCGCGGACTCCGACCGCGCGACGCTCCTCCGGCGCCGTCCGATCTCGACGGTAACAGCAGCGGCTCCGATTCGGAGTCGATTACGTATAAGAGCCGAAACGCGTTGAAGCGCGACGCTCGGCGCGCCGTGCGTTGGGCTATGGACCTCGCTTCGTTCTCTACGCCTCAGCTCAAATTGATTATCAGAGTGGCGTCTTTGGACGAAGATGTATTGGATGCTGTTATGTTAGTGAAGAGATTTGGGAATGATGTGCGAGAAGGAAAGCGAAGGCAGTACAATTATATTGCAAAACTGTTACGGGATGCGGACACCGAGCTGATGGATGCTTTGATTCGGGCTACAAAAGACAGTGATCAAAAGAAGCTGCAAGACTTATGTGGTTCGGAGGCGTTGAGCATTGATGATGAGGAAGAAGAAGAAGCTGAAGAATCTGATAACGAGGAAGAAGAGGGTTCTCACATTGAGGTAGCATCCAGATGGGTTGAGGGTATGATCAATAAGGATGTTGATATCACCAATGAAGTTTATTCAATTTCTGATGTTGAGTTTGATCGTCAGGAATTGCGTAAACTTGTCCGGAAAGTACATTTGGCTTTGGAACGCTCAAGCATTTCTGAGGAAAACAAGGAAAAGATTGATGAAGCGGTAGTGGGTGCCAAAAAATCACTCACCAGGTTCCTTCGTGCCCTTGCTAAAAGCAGCCTAATGATCAATCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF615

Protein Analysis

301

Amino Acids

33.66

Weight (kDa)

5.72

Isoelectric Point (pI)

57.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DarP PF04751 90 - 270 9.1e-26 Dual-action ribosomal maturation protein DarP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24175 AT4G24175
fragaria_vesca FvH4_2g38330
malus_domestica MD08G1011800.v1.1 MD08G1012000.v1.1
prunus_persica Prupe.1G364300_v2.0.a1
pyrus_communis pycom08g01020 pycom08g01050 pycom15g00990
rosa_chinensis RchiOBHm_Chr6g0303031
rosa_laevigata RLG00000011114
rosa_multiflora Rmu_ssc0000460.1_g000031
rosa_roxburghii Rroxscaffold_7G00165100
rosa_rugosa Rorug06G0318800.1 Rorug06G0318900.1
rosa_samantha Rh6AG432400 Rh6BG470200 Rh6CG444800 Rh6DG431500
rosa_wichuraiana Rw6G037480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 214, 836
AccII CGCG 8 cut(s) 132, 165, 186, 199, 201, 284, 294, 306
AccIII TCCGGA 1 cut(s) 759
AcuI CTGAAG 1 cut(s) 627
AcyI GRCGYC 3 cut(s) 168, 215, 374
AfaI GTAC 2 cut(s) 458, 768
AfiI CCNNNNNNNGG 2 cut(s) 131, 315
AflIII ACRYGT 1 cut(s) 282
AgsI TTSAA 2 cut(s) 289, 714
AhlI ACTAGT 1 cut(s) 178
AjnI CCWGG 1 cut(s) 854
AjuI GAANNNNNNNTTGG 2 cut(s) 755, 787
AluBI AGCT 5 cut(s) 158, 352, 499, 547, 605
AluI AGCT 5 cut(s) 158, 352, 499, 547, 605
Alw21I GWGCWC 1 cut(s) 113
Alw26I GTCTC 1 cut(s) 10
AlwNI CAGNNNCTG 1 cut(s) 52
Aor13HI TCCGGA 1 cut(s) 759
ApeKI GCWGC 6 cut(s) 43, 46, 57, 240, 547, 882
AscI GGCGCGCC 1 cut(s) 304
AspLEI GCGC 6 cut(s) 43, 201, 217, 294, 306, 308
AspS9I GGNCC 1 cut(s) 325
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 2 cut(s) 688, 844
AvaII GGWCC 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 871
BanI GGYRCC 2 cut(s) 214, 836
Bbv12I GWGCWC 1 cut(s) 113
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 6 cut(s) 30, 44, 58, 252, 534, 894
BccI CCATC 2 cut(s) 496, 654
BceAI ACGGC 2 cut(s) 202, 293
BciT130I CCWGG 1 cut(s) 856
BclI TGATCA 3 cut(s) 535, 675, 891
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 10
BcuI ACTAGT 1 cut(s) 178
BfaI CTAG 1 cut(s) 179
BfmI CTRYAG 1 cut(s) 44
BfoI RGCGCY 2 cut(s) 44, 218
BisI GCNGC 9 cut(s) 44, 47, 58, 130, 166, 241, 244, 548, 883
BlsI GCNGC 9 cut(s) 45, 48, 59, 131, 167, 242, 245, 549, 884
Bme1390I CCNGG 2 cut(s) 38, 856
Bme18I GGWCC 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 325
BmiI GGNNCC 4 cut(s) 216, 247, 838, 860
BmrFI CCNGG 2 cut(s) 38, 856
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 4 cut(s) 388, 475, 496, 662
BmuI ACTGGG 1 cut(s) 20
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 772
BpuMI CCSGG 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 267
BsaHI GRCGYC 3 cut(s) 168, 215, 374
BsaJI CCNNGG 2 cut(s) 130, 184
BsaWI WCCGGW 2 cut(s) 142, 759
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 315
Bse1I ACTGG 1 cut(s) 15
Bse3DI GCAATG 1 cut(s) 38
BseAI TCCGGA 1 cut(s) 759
BseBI CCWGG 1 cut(s) 856
BseDI CCNNGG 2 cut(s) 130, 184
BseGI GGATG 5 cut(s) 403, 490, 511, 653, 691
BseLI CCNNNNNNNGG 2 cut(s) 131, 315
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 3 cut(s) 98, 362, 789
BseNI ACTGG 1 cut(s) 15
BsePI GCGCGC 1 cut(s) 304
BseRI GAGGAG 1 cut(s) 198
BseSI GKGCMC 1 cut(s) 871
BseXI GCAGC 6 cut(s) 30, 44, 58, 252, 534, 894
Bsh1236I CGCG 8 cut(s) 132, 165, 186, 199, 201, 284, 294, 306
Bsh1285I CGRYCG 1 cut(s) 197
BshNI GGYRCC 2 cut(s) 214, 836
BsiEI CGRYCG 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 113
BsiSI CCGG 4 cut(s) 38, 143, 212, 760
BslI CCNNNNNNNGG 2 cut(s) 131, 315
BsmAI GTCTC 1 cut(s) 10
Bsp1286I GDGCHC 2 cut(s) 113, 871
Bsp13I TCCGGA 1 cut(s) 759
Bsp143I GATC 5 cut(s) 223, 535, 675, 733, 891
BspCNI CTCAG 3 cut(s) 99, 361, 790
BspEI TCCGGA 1 cut(s) 759
BspFNI CGCG 8 cut(s) 132, 165, 186, 199, 201, 284, 294, 306
BspLI GGNNCC 4 cut(s) 216, 247, 838, 860
BspMAI CTGCAG 1 cut(s) 48
BspT107I GGYRCC 2 cut(s) 214, 836
BsrDI GCAATG 1 cut(s) 38
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 2 cut(s) 130, 184
BssHII GCGCGC 1 cut(s) 304
BssMI GATC 5 cut(s) 223, 535, 675, 733, 891
BssNI GRCGYC 3 cut(s) 168, 215, 374
Bst2UI CCWGG 1 cut(s) 856
Bst4CI ACNGT 3 cut(s) 233, 477, 533
Bst6I CTCTTC 3 cut(s) 89, 410, 624
BstACI GRCGYC 3 cut(s) 168, 215, 374
BstBAI YACGTR 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 4 cut(s) 107, 154, 348, 798
BstDSI CCRYGG 2 cut(s) 130, 184
BstF5I GGATG 5 cut(s) 403, 490, 511, 653, 691
BstFNI CGCG 8 cut(s) 132, 165, 186, 199, 201, 284, 294, 306
BstH2I RGCGCY 2 cut(s) 44, 218
BstHHI GCGC 6 cut(s) 43, 201, 217, 294, 306, 308
BstKTI GATC 5 cut(s) 226, 538, 678, 736, 894
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 5 cut(s) 223, 535, 675, 733, 891
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 5 cut(s) 28, 63, 212, 305, 310
BstNI CCWGG 1 cut(s) 856
BstSCI CCNGG 2 cut(s) 36, 854
BstSFI CTRYAG 1 cut(s) 44
BstSLI GKGCMC 1 cut(s) 871
BstSNI TACGTA 1 cut(s) 267
BstUI CGCG 8 cut(s) 132, 165, 186, 199, 201, 284, 294, 306
BstV1I GCAGC 6 cut(s) 30, 44, 58, 252, 534, 894
BtgI CCRYGG 2 cut(s) 130, 184
BtsCI GGATG 5 cut(s) 403, 490, 511, 653, 691
BtsIMutI CAGTG 3 cut(s) 71, 104, 538
Cac8I GCNNGC 1 cut(s) 306
CaiI CAGNNNCTG 1 cut(s) 52
CfoI GCGC 6 cut(s) 43, 201, 217, 294, 306, 308
Cfr13I GGNCC 1 cut(s) 325
Cfr42I CCGCGG 2 cut(s) 133, 187
CseI GACGC 5 cut(s) 55, 157, 212, 305, 363
CsiI ACCWGGT 1 cut(s) 854
Csp6I GTAC 2 cut(s) 457, 767
CviQI GTAC 2 cut(s) 457, 767
DdeI CTNAG 4 cut(s) 107, 154, 348, 798
DinI GGCGCC 1 cut(s) 216
DpnI GATC 5 cut(s) 225, 537, 677, 735, 893
DpnII GATC 5 cut(s) 223, 535, 675, 733, 891
Eam1104I CTCTTC 3 cut(s) 89, 410, 624
EarI CTCTTC 3 cut(s) 89, 410, 624
Eco105I TACGTA 1 cut(s) 267
Eco32I GATATC 1 cut(s) 694
Eco47I GGWCC 1 cut(s) 325
Eco57I CTGAAG 1 cut(s) 627
EcoRII CCWGG 1 cut(s) 854
EcoRV GATATC 1 cut(s) 694
EgeI GGCGCC 1 cut(s) 216
EheI GGCGCC 1 cut(s) 216
FaiI YATR 8 cut(s) 174, 270, 323, 407, 465, 559, 674, 901
FauI CCCGC 1 cut(s) 134
FbaI TGATCA 3 cut(s) 535, 675, 891
Fnu4HI GCNGC 9 cut(s) 44, 47, 58, 130, 166, 241, 244, 548, 883
FokI GGATG 5 cut(s) 410, 497, 518, 640, 698
Fsp4HI GCNGC 9 cut(s) 44, 47, 58, 130, 166, 241, 244, 548, 883
FspBI CTAG 1 cut(s) 179
GlaI GCGC 6 cut(s) 42, 200, 216, 293, 305, 307
GluI GCNGC 9 cut(s) 44, 47, 58, 130, 166, 241, 244, 548, 883
HaeII RGCGCY 2 cut(s) 44, 218
HapII CCGG 4 cut(s) 38, 143, 212, 760
HgaI GACGC 5 cut(s) 55, 157, 212, 305, 363
HhaI GCGC 6 cut(s) 43, 201, 217, 294, 306, 308
Hin1I GRCGYC 3 cut(s) 168, 215, 374
Hin6I GCGC 6 cut(s) 41, 199, 215, 292, 304, 306
HinP1I GCGC 6 cut(s) 41, 199, 215, 292, 304, 306
HinfI GANTC 6 cut(s) 76, 189, 251, 257, 514, 611
HpaII CCGG 4 cut(s) 38, 143, 212, 760
HphI GGTGA 2 cut(s) 688, 844
Hpy166II GTNNAC 1 cut(s) 752
Hpy188I TCNGA 9 cut(s) 194, 223, 250, 256, 368, 568, 616, 721, 799
Hpy188III TCNNGA 4 cut(s) 227, 657, 740, 760
Hpy8I GTNNAC 1 cut(s) 752
Hpy99I CGWCG 4 cut(s) 71, 206, 233, 299
HpyAV CCTTC 3 cut(s) 434, 444, 872
HpyCH4III ACNGT 3 cut(s) 233, 477, 533
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 3 cut(s) 46, 470, 550
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 63, 212, 305, 310
HpyF3I CTNAG 4 cut(s) 107, 154, 348, 798
HpySE526I ACGT 1 cut(s) 266
Hsp92I GRCGYC 3 cut(s) 168, 215, 374
HspAI GCGC 6 cut(s) 41, 199, 215, 292, 304, 306
KasI GGCGCC 1 cut(s) 214
Kpn2I TCCGGA 1 cut(s) 759
Ksp22I TGATCA 3 cut(s) 535, 675, 891
KspI CCGCGG 2 cut(s) 133, 187
Kzo9I GATC 5 cut(s) 223, 535, 675, 733, 891
LmnI GCTCC 3 cut(s) 65, 211, 251
Lsp1109I GCAGC 6 cut(s) 30, 44, 58, 252, 534, 894
LweI GCATC 4 cut(s) 388, 475, 496, 662
MabI ACCWGGT 1 cut(s) 854
MaeI CTAG 1 cut(s) 179
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 2 cut(s) 233, 477
MalI GATC 5 cut(s) 225, 537, 677, 735, 893
MboI GATC 5 cut(s) 223, 535, 675, 733, 891
MhlI GDGCHC 2 cut(s) 113, 871
MluCI AATT 4 cut(s) 356, 460, 714, 743
MluI ACGCGT 1 cut(s) 282
Mly113I GGCGCC 1 cut(s) 215
MlyI GAGTC 2 cut(s) 183, 266
MmeI TCCRAC 1 cut(s) 217
MroI TCCGGA 1 cut(s) 759
MslI CAYNNNNRTG 1 cut(s) 658
MspA1I CMGCKG 3 cut(s) 132, 186, 243
MspI CCGG 4 cut(s) 38, 143, 212, 760
MspR9I CCNGG 2 cut(s) 38, 856
MvaI CCWGG 1 cut(s) 856
MvnI CGCG 8 cut(s) 132, 165, 186, 199, 201, 284, 294, 306
MwoI GCNNNNNNNGC 5 cut(s) 28, 63, 212, 305, 310
NarI GGCGCC 1 cut(s) 215
NciI CCSGG 1 cut(s) 38
NdeII GATC 5 cut(s) 223, 535, 675, 733, 891
NlaIV GGNNCC 4 cut(s) 216, 247, 838, 860
NmeAIII GCCGAG 1 cut(s) 280
PalAI GGCGCGCC 1 cut(s) 304
PauI GCGCGC 1 cut(s) 304
PfeI GAWTC 4 cut(s) 76, 251, 514, 611
PkrI GCNGC 9 cut(s) 45, 48, 59, 131, 167, 242, 245, 549, 884
PleI GAGTC 2 cut(s) 183, 265
PluTI GGCGCC 1 cut(s) 218
PpsI GAGTC 2 cut(s) 183, 265
Ppu21I YACGTR 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 854
PspGI CCWGG 1 cut(s) 854
PspN4I GGNNCC 4 cut(s) 216, 247, 838, 860
PspPI GGNCC 1 cut(s) 325
PstI CTGCAG 1 cut(s) 48
PstNI CAGNNNCTG 1 cut(s) 52
PteI GCGCGC 1 cut(s) 304
RsaI GTAC 2 cut(s) 458, 768
RsaNI GTAC 2 cut(s) 457, 767
RseI CAYNNNNRTG 1 cut(s) 658
SacII CCGCGG 2 cut(s) 133, 187
SatI GCNGC 9 cut(s) 44, 47, 58, 130, 166, 241, 244, 548, 883
Sau3AI GATC 5 cut(s) 223, 535, 675, 733, 891
Sau96I GGNCC 1 cut(s) 325
SchI GAGTC 2 cut(s) 183, 266
ScrFI CCNGG 2 cut(s) 38, 856
SduI GDGCHC 2 cut(s) 113, 871
SexAI ACCWGGT 1 cut(s) 854
SfaNI GCATC 4 cut(s) 388, 475, 496, 662
SfcI CTRYAG 1 cut(s) 44
SfoI GGCGCC 1 cut(s) 216
Sfr303I CCGCGG 2 cut(s) 133, 187
SgrBI CCGCGG 2 cut(s) 133, 187
SgsI GGCGCGCC 1 cut(s) 304
SinI GGWCC 1 cut(s) 325
SmiMI CAYNNNNRTG 1 cut(s) 658
SmlI CTYRAG 1 cut(s) 787
SmoI CTYRAG 1 cut(s) 787
SnaBI TACGTA 1 cut(s) 267
SpeI ACTAGT 1 cut(s) 178
Sse9I AATT 4 cut(s) 356, 460, 714, 743
SspDI GGCGCC 1 cut(s) 214
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 36, 854
TaaI ACNGT 3 cut(s) 233, 477, 533
TaiI ACGT 1 cut(s) 269
TaqI TCGA 2 cut(s) 228, 260
TasI AATT 4 cut(s) 356, 460, 714, 743
TatI WGTACW 2 cut(s) 456, 766
TauI GCSGC 3 cut(s) 132, 168, 246
TfiI GAWTC 4 cut(s) 76, 251, 514, 611
TscAI CASTG 3 cut(s) 78, 111, 538
TseI GCWGC 6 cut(s) 43, 46, 57, 240, 547, 882
TspDTI ATGAA 2 cut(s) 717, 837
TspRI CASTG 3 cut(s) 78, 111, 538
VpaK11BI GGWCC 1 cut(s) 325
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.